[Serological and molecular methods in diagnostics of primary acute hepatitis caused with human herpesvirus type 6 - a case report].

Medycyna doswiadczalna i mikrobiologia Pub Date : 2017-01-01

Marta Rybka, Maciej Przybylski, Paulina Machura, Tomasz Dzieciątkowski, Rafal Krenke', Marta Wrólewska

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Abstract

Introduction: HHV-6 has been identified as the etiologic agent of exanthema subitum in infants and acute febrile illness in young children, although primary HHV-6 infection in immunocompetent adolescents and adults is very rare. We report the case of acute hepatitis in an 18-year-old man without any immunological dysfunctions.

Material and methods: Full virological examination of sera samples was performed. Specimens were tested for the presence of specific anti-EBV and anti-HHV-6 antibodies and also for the presence of DNA of other herpesviruses, using qPCR assays.

Results: Obtained results demonstrated the presence of HHV-6 DNA in all examined sera samples and IgM-class antibodies against HHV-6 in the second specimen. No other etiologic agents were found that are known to induce hepatitis. Hence, patient was diagnosed as having acute hepatitis triggered by HHV-6.

Conclusions: We found that qPCR is a very useful tool for the detection of different herpesvirus infections, especially with low-copy viraemia in clinical specimens.

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[用血清学和分子方法诊断6型人疱疹病毒引起的原发性急性肝炎1例报告]。

简介:HHV-6已被确定为婴儿麻疹和幼儿急性发热性疾病的病原，尽管在免疫功能正常的青少年和成人中原发性HHV-6感染非常罕见。我们报告一例急性肝炎在一个18岁的男子没有任何免疫功能障碍。材料和方法:对血清样本进行全面病毒学检查。使用qPCR法检测标本是否存在特异性抗ebv和抗hhv -6抗体，以及是否存在其他疱疹病毒的DNA。结果:获得的结果表明，所有检测的血清样本中都存在HHV-6 DNA，第二个样本中存在抗HHV-6的igm类抗体。没有发现其他已知的病原体可诱发肝炎。因此，患者被诊断为由HHV-6引发的急性肝炎。结论:我们发现qPCR是一种非常有用的工具，用于检测不同的疱疹病毒感染，特别是临床标本中的低拷贝病毒血症。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Medycyna doswiadczalna i mikrobiologia

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