{"title":"[Analysis of the genetic background and mechanisms of dissemination of resistance to tetracycline of the Polish population of Campylobacter strains isolated in Poland in years 2007-2008].","authors":"Tomasz Wołkowicz, Jolanta Szych, Sebastian Wardak","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>In Poland, constant rise of number of Campylobacter strains resistant to tetracycline is observed in Poland. Analysis of the resistant strains showed their strong diversity, including both the different levels of resistance to this drug, large differences in the sequence of the resistant gene tetO, and diverse phylogenetic origin. The study also confirmed the important role of horizontal spread of resistance which, in the event of such a large diversity of resistant strains, can cause further very rapid escalation of resistance of Campylobacter to tetracycline.</p>","PeriodicalId":18521,"journal":{"name":"Medycyna doswiadczalna i mikrobiologia","volume":"63 4","pages":"305-14"},"PeriodicalIF":0.0,"publicationDate":"2011-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30502209","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"[Sensitivity assessment of thyme and lavender essential oils against clinical strains of Escherichia coli for their resistance].","authors":"Monika Sienkiewicz, Danuta Kalemba, Małgorzata Wasiela","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Strong antiseptic activity of plant essential oils and extracts has been known for a long time. The antibacterial activity of thyme and lavender essential oils were tested against 30 clinical bacterial strains of Escherichia coli from patients with different clinical conditions. The agar diffusion method was used for microbial growth inhibition at various concentrations of the oils from Thymus vulgaris and Lavandula angustifolia. Susceptibility testing to antibiotics and chemotherapeutics was carried out using disc-diffusion method. The results of experiments showed that the both oils, from T. vulgaris and L. angustifolia were active against all of the clinical strains, but thyme oil demonstrated the highest activity. Thyme and lavender essential oils were active against multi drug resistant clinical strains of Escherichia coli genera. The results of experiments justify a study related to activity other essential oils against different genus of bacteria.</p>","PeriodicalId":18521,"journal":{"name":"Medycyna doswiadczalna i mikrobiologia","volume":"63 3","pages":"273-81"},"PeriodicalIF":0.0,"publicationDate":"2011-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30337321","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"[Susceptibility of Klebsiella pneumoniae isolated from urinary tract infections to quinolones].","authors":"Alicja Sekowska, Justyna Pluta, Kamila Buzała, Eugenia Gospodarek","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Urinary Tract Infections (UTIs) are the most prevalent infections worldwide both in males and females. K. pneumoniae is one of the major pathogens causing UTIs. Fluoroquinolones are effective drugs for treating infections caused by Klebsiella spp. The aim of this study was to evaluated the susceptibility to three fluoroquinolones of K. pneumoniae strains isolated from urine. The MICs of ciprofloxacin was determined by agar dilution method and the MICs of norfloxacin and gatifloxacin by E-test. Among analysed K. pneumoniae strains 86.7% was susceptible to gatifloxacine, 76.7% to norfloxacin and 51.2% to ciprofloxacine.</p>","PeriodicalId":18521,"journal":{"name":"Medycyna doswiadczalna i mikrobiologia","volume":"63 1","pages":"53-7"},"PeriodicalIF":0.0,"publicationDate":"2011-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30338075","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Barbara Rózalska, Beata Sadowska, Marzena Wieckowska-Szakiel, Aleksandra Budzyńska
{"title":"[The synergism of antifungals and essential oils against Candida spp. evaluated by a modified gradient-diffusion method].","authors":"Barbara Rózalska, Beata Sadowska, Marzena Wieckowska-Szakiel, Aleksandra Budzyńska","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The usefulness of modified method of MIC Test Strip, for determining the synergistic effect of essential oils in the liquid or volatile phase with fluconazole and voriconazole, was evaluated. Geranium oil used against C. albicans in agar dilution test, at a concentration of 1/2 MIC caused a drop in the value of fluconazole MIC from 12.0 mg/l to 0.064 mg/l and voriconazole from 0.125 mg/l to 0.006 mg/l. A similar effect of drug combinations with essential oils was obtained in the case of C. glabrata study. Volatile Clove oil and cytronelal, applied in subMIC concentrations, also caused a reduction offluconazole and voriconazole MICs. Thus, utility of this simple methods developed by us for testing the effectiveness of combinations of known drugs and new compounds with antifungal activity, has been confirmed.</p>","PeriodicalId":18521,"journal":{"name":"Medycyna doswiadczalna i mikrobiologia","volume":"63 2","pages":"163-9"},"PeriodicalIF":0.0,"publicationDate":"2011-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30337913","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"[Susceptibility to antifungal agents of Candida sp. and biofilm formation].","authors":"Emilia Ciok-Pater, Agata Białucha, Eugenia Gospodarek, Agnieszka Ostafin","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>In recent years the increase in frequency of fungal infections with Candida sp. was noticed. These infections are connected with ability of Candida sp. to form biofilm on surfaces of biomaterials used in medicine. Furthermore fungal infections make serious therapeutic problems because ofbiofilm resistance to antifungal agents actually. The aim of the study was to evaluate the susceptibility to antifungal agents of Candida sp. and their ability to form biofilm on different biomaterials. 50 strains of Candida sp. isolated from patients of University Hospital No. 1 of dr A. Jurasz in Bydgoszcz were examined. API Candida (bioMérieux) tests were used to identify Candida sp. strains. The susceptibility of the yeast strains to antifungal agents was evaluated by ATB FUNGUS 2 INT (bioMérieux) tests. The susceptibility of examined strains to voriconazole, posaconazole, caspofungin and anidulafungin was assessed by means ofEtests (AB BIODISK) method employing drug concentrations from 0,002 to 32 microg/ml. All analysed strains were susceptible to amphotericin B and caspofungin. Biofilm formation on different biomaterials (silicon, latex, polychloride vinyl, polypropylene, nylon) was measured after 72 hour incubation at 37 degrees C. All examined yeasts formed biofilm on all analysed biomaterials. The highest number of strains formed biofilm on surface of polychloride vinyl: 23 (92,0%) by C. albicans strains and 24 (96,0%) Candida non-albicans strains. The lowest number of the strains formed biofilm on the surface of nylon: 12 (48,0%) of C. albicans strains and 9 (36,0%) of Candida non-albicans strains. The studied strains resistant to azoles and anidulafungin display stronger ability to form biofilm on surfaces of all analysed biomaterials.</p>","PeriodicalId":18521,"journal":{"name":"Medycyna doswiadczalna i mikrobiologia","volume":"63 2","pages":"171-87"},"PeriodicalIF":0.0,"publicationDate":"2011-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30337914","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"[The evaluation of lipolytic activity of strains of Staphylococcus epidermidis and Staphylococcus haemolyticus].","authors":"Joanna Wróblewska, Alicja Sekowska, Patrycja Zalas-Wiecek, Eugenia Gospodarek","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>A total of 103 isolates of CNS (66 strains of S. epidermidis and 37 strains of S. haemolyticus) were investigated. Lipolytic activity of staphylococcal strains was determined by Tryptic Soy Agar containing Tween 20 or Tween 60. The 95.4% strains of staphylococci demonstrated the lipolytic activity on Tween 20 agar and the 89.4% of strains of staphylococci degradation ester of fatty acids on Tweens 60 agar. We detected that S. epidermidis strains (respectively 95,4%, 89,4%) produced lipases more frequently than S. haemolyticus strains (respectively 72,9%, 59,4%). Studies suggest that source of isolation from clinical materials (blood, wound and pus) does not have an influence on the ability hydrolysis esters.</p>","PeriodicalId":18521,"journal":{"name":"Medycyna doswiadczalna i mikrobiologia","volume":"63 2","pages":"99-103"},"PeriodicalIF":0.0,"publicationDate":"2011-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30338022","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Sylwia Kozuszko, Agata Białucha, Tomasz Bogiel, Eugenia Gospodarek
{"title":"[High level of aminoglycoside resistance among Enterococcus faecalis and Enterococcus faecium strains].","authors":"Sylwia Kozuszko, Agata Białucha, Tomasz Bogiel, Eugenia Gospodarek","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Enterococcus sp. strains are believed as important reason of serious nosocomial infections currently. These infections are cured by using combination of beta-lactams and aminoglycosides for their treatment. Enterococcus sp. resistant to high-level doses of aminoglycosides, beta-lactams and vancomycin are responsible for therapeutic failure. The aim of our study was to evaluate the incidence of isolation and susceptibility to antibiotics of HLAR Enterococcus sp. strains isolated between 2007 and 2010 from the patients of University Hospital No. 1 of dr A. Jurasz Collegium Medicum of L. Rydygier in Bydgoszcz Nicolaus Copernicus University in Toruń. Amongst 6137 Enterococcus sp. strains 1124 (18,3%) presented HLAR phenotype; 53,1% of them was identified as E. faecalis and 46,9% as E. faecium. The highest percentage of all examined strains was isolated from the patients of different surgery clinics, Intensive Care Units, and Pediatrics, Hematology and Oncology Clinic. HLAR and HLSR phenotypes were noted in E. faecalis, for 45,7% and 27,5% strains, in E. faecium - 29,8% and 9,5%, respectively. HLGR phenotype was presented twice more often in E. faecium than E. faecalis. Highest percentages of E. faecium resistant to glycopeptides and rifampicin were observed when compared with E. faecalis. The highest percentages of strains intermediate, resistant to vancomycin and resistant to glycopeptides were noted for E. faecium strains with phenotypes HLAR, HLGR and HLSR.</p>","PeriodicalId":18521,"journal":{"name":"Medycyna doswiadczalna i mikrobiologia","volume":"63 2","pages":"105-13"},"PeriodicalIF":0.0,"publicationDate":"2011-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30338023","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Joanna Kwiecińska-Piróg, Tomasz Bogiel, Eugenia Gospodarek, Mariusz Kasprzak, Leszek Jerzak, Piotr Kamiński
{"title":"[The susceptibility of Proteus mirabilis strains isolated from white stork (Ciconia ciconia)].","authors":"Joanna Kwiecińska-Piróg, Tomasz Bogiel, Eugenia Gospodarek, Mariusz Kasprzak, Leszek Jerzak, Piotr Kamiński","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Proteus sp. rods are ubiquitous bacteria, widespread in the environment and classified also as opportunistic human pathogens. The aim of our study was to evaluate susceptibility of Proteus mirabilis strains isolated from white stork (Ciconia ciconia) regarding as his natural bacterial flora, compare and discuss their results with data obtained from scientific literature for clinical strains of the same species. Susceptibility of 59 P. mirabilis strains was estimated for 27 antimicrobials using disc-diffusion method and the ability to produce extended spectrum beta-lactamases was evaluated by double disc synergy test. Environmental P. mirabilis strains isolated from white stork were assessed as more susceptible to most of the examined antimicrobials and production of extended spectrum beta-lactamases was not noted amongst them.</p>","PeriodicalId":18521,"journal":{"name":"Medycyna doswiadczalna i mikrobiologia","volume":"63 2","pages":"139-44"},"PeriodicalIF":0.0,"publicationDate":"2011-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30338027","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Monika Brzychczy-Włoch, Tomasz Gosiewski, Małgorzata Bodaszewska, Katarzyna Talaga, Joanna Natkaniec, Paweł Adamski, Piotr B Heczko
{"title":"[Occurrence of surface protein genes from alpha-like protein (Alp) family in Streptococcus agalactiae isolates].","authors":"Monika Brzychczy-Włoch, Tomasz Gosiewski, Małgorzata Bodaszewska, Katarzyna Talaga, Joanna Natkaniec, Paweł Adamski, Piotr B Heczko","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Distribution of serotypes and alpha-like surface protein (Alp) of Streptococcus agalactiae (Group B Streptococci - GBS) vary with geographical region, ethnic origin and the virulence of clinical isolates. Demonstration of different genotypes based on surface protein genes improves the potential of GBS subtyping, is essential in research on new vaccines against invasive neonatal infections and may be useful in epidemiological studies. The molecular characterization of protein gene profile of GBS isolates was the main aim of this study. We evaluated the applicability of multiplex PCR for the identification of GBS protein genes from Alp family, such as: epsilon, bca, rib, alp2, alp3, alp4 and evaluated presence of these genes in the group of GBS isolates originating from vaginal or rectal carriage in pregnant women. For statistical analysis the G2 (Likelihood ratio) test was used. P values of < 0.05 were considered significant. The surface protein genes were found in all investigated strains. The epsilon gene dominated (27%) in GBS isolates originating from healthy pregnant women. The other genes were detected with the following frequency: rib (21%), alp2 (21%), bca (17%) and alp3 (14%). In the analyzed population, GBS strains with alp4 gene were not found. A statistically significant relationship between surface protein genes and capsular polysaccharides was demonstrated (p < 0.0001). The results of our study show immense diagnostic usefulness of multiplex PCR for identification of genes encoding GBS surface proteins from Alp family.</p>","PeriodicalId":18521,"journal":{"name":"Medycyna doswiadczalna i mikrobiologia","volume":"63 1","pages":"5-14"},"PeriodicalIF":0.0,"publicationDate":"2011-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30338173","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ewa Szczuka, Jolanta Prawda-Zołotar, Maryla Nowakiewicz, Adam Kaznowski
{"title":"[Antimicrobial sensivity and ability to slime production of koagulaze-negative staphylococci].","authors":"Ewa Szczuka, Jolanta Prawda-Zołotar, Maryla Nowakiewicz, Adam Kaznowski","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The aim of this study was to assess the ability of slime production ofcoagulase-negative staphylococci (CONS) and evaluate the susceptibility of bacteria to antibiotics. Strains were isolated from clinical specimens obtained from hospitalized patients. The most frequently isolated species were S. epidermidis (51%), S. hominis (18%), S. haemolyticus (13%). The result of this study shows that 61% of S.epidermidis produce slime on CRA (Congo red agar), whereas none of the tested S. haemolyticus strains has this ability. All examined strains were susceptible to vancomycin, linezolid and quinupristin/ dalfopristin. The majority of strains were susceptible to minocycline, fusid acid, nitrofurantoin and rifampicin. Sixty six percent of isolates were determined as methicillin-resistant coagulase-negative staphylococci.</p>","PeriodicalId":18521,"journal":{"name":"Medycyna doswiadczalna i mikrobiologia","volume":"63 1","pages":"15-9"},"PeriodicalIF":0.0,"publicationDate":"2011-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30338174","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}