IF 2.2 3区工程技术

Micron Pub Date : 2026-02-01 Epub Date: 2025-11-14 DOI: 10.1016/j.micron.2025.103957

Yifan Wang , Mai Tan , Carlos Fernandez-Granda , Peter A. Crozier

{"title":"Revealing information from weak signals in electron energy-loss spectroscopy with a a deep denoiser","authors":"Yifan Wang , Mai Tan , Carlos Fernandez-Granda , Peter A. Crozier","doi":"10.1016/j.micron.2025.103957","DOIUrl":"10.1016/j.micron.2025.103957","url":null,"abstract":"<div><div>Electron energy-loss spectroscopy (EELS) coupled with scanning transmission electron microscopy (STEM) is a powerful technique to determine materials composition and bonding with high spatial resolution. Noise is often a limitation especially with the increasing sophistication of EELS experiments. The signal characteristics from direct electron detectors provide a new opportunity to design superior denoisers. We have developed a CNN based denoiser, the unsupervised deep video denoiser (UDVD), which can be applied to EELS datasets acquired with direct electron detectors. We described UDVD and explained how to adapt the denoiser to energy-loss spectral series. To benchmark the performance of the denoiser on EELS datasets, we generated and denoised a set of simulated spectra. We demonstrate the charge spreading effect associated with pixel interfaces on direct electron detectors, which leads to artifacts after denoising. To suppress such artifacts, we propose some adjustments. We demonstrate the effectiveness of the denoiser using two challenging real data examples: mapping Gd dopants in CeO₂ nanoparticles and characterizing vibrational modes in hexagonal boron nitride (h-BN) with atomic resolution.</div></div>","PeriodicalId":18501,"journal":{"name":"Micron","volume":"201 ","pages":"Article 103957"},"PeriodicalIF":2.2,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145596813","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phase imaging and analysis of the annihilation of a dislocation at a crystal surface 晶体表面位错湮灭的相位成像与分析。

IF 2.2 3区工程技术

Micron Pub Date : 2026-02-01 Epub Date: 2025-11-03 DOI: 10.1016/j.micron.2025.103945

Rodney A. Herring

引用次数: 0

Morphology of the male reproductive system and sperm of Discodon minutum Pic, 1928 (Coleoptera: Cantharidae) 1928年斑点蝶（Discodon minutum Pic）雄性生殖系统和精子的形态（鞘翅目：斑蝥科）

IF 2.2 3区工程技术

Micron Pub Date : 2026-02-01 Epub Date: 2025-11-22 DOI: 10.1016/j.micron.2025.103962

Francisco Witallo S. Nascimento , Maurício S. Paulo , Dayvson A. Costa , Paulo H. Rezende , Glenda Dias , José Lino-Neto

{"title":"Morphology of the male reproductive system and sperm of Discodon minutum Pic, 1928 (Coleoptera: Cantharidae)","authors":"Francisco Witallo S. Nascimento , Maurício S. Paulo , Dayvson A. Costa , Paulo H. Rezende , Glenda Dias , José Lino-Neto","doi":"10.1016/j.micron.2025.103962","DOIUrl":"10.1016/j.micron.2025.103962","url":null,"abstract":"<div><div>The morphology of the male reproductive system and sperm of adults of <em>Discodon minutum</em> Pic, 1928 (Elateriformia: Cantharidae) was described using light microscopy (LM) and transmission electron microscopy (TEM). The morphology of the reproductive system and sperm provides information of taxonomic value and reveals aspects of reproductive biology, although such morphological characteristics are poorly understood in Cantharidae. The reproductive system of <em>D. minutum</em> consists of two free testes, each with 28–30 follicles, two vasa deferentia, and three pairs of accessory glands, as well as an ejaculatory duct with its median portion functioning as an ejaculatory pump. It also has two atypical seminal vesicles formed by evaginations at the base of the vasa deferentia, which serve as sperm storage and secretion. The follicles contain developed cyst cells and mature sperm, grouped in a spermatodesma-like cystic bundle. However, sperm are found free and immersed in secretions within the seminal vesicles, whose wall folds indicate an intense exchange of substances, potentially involved in sperm maintenance, with the hemolymph. The sperm ultrastructure resembles that observed in other Elateriformia, characterized by a nucleus positioned laterally to the flagellar components and a similar arrangement of the mitochondrial derivatives and axoneme. However, they differ in the asymmetrical position of the dense bodies and the triangular cross-sectional shape of the dense bodies and the nucleus. Histological confirmation of an ejaculatory pump in <em>D. minutum</em> is the first report of this structure in Elateroidea. Previous descriptions in Elateridae may have misidentified the ejaculatory pumps as “accessory glands of the ejaculatory duct”.</div></div>","PeriodicalId":18501,"journal":{"name":"Micron","volume":"201 ","pages":"Article 103962"},"PeriodicalIF":2.2,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145620882","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Application of position-averaged convergent beam electron diffraction to determine the thickness of ultra-thin materials 位置平均会聚束电子衍射在超薄材料厚度测定中的应用

IF 2.2 3区工程技术

Micron Pub Date : 2026-02-01 Epub Date: 2025-11-22 DOI: 10.1016/j.micron.2025.103963

Johannes Biskupek , Philipp Eltgen , Ute Kaiser

{"title":"Application of position-averaged convergent beam electron diffraction to determine the thickness of ultra-thin materials","authors":"Johannes Biskupek , Philipp Eltgen , Ute Kaiser","doi":"10.1016/j.micron.2025.103963","DOIUrl":"10.1016/j.micron.2025.103963","url":null,"abstract":"<div><div>The precise thickness determination of free-standing two-dimensional (2D) materials—such as few-layer graphene and molybdenum disulfide (MoS₂)—is achieved using position-averaged convergent beam electron diffraction (PACBED). Experimental PACBED patterns are quantitatively compared with simulated patterns using two distinct evaluation methods: (1) a difference value method (DVM) based on pixel-wise contrast comparison, and (2) a convolutional neural network (CNN) trained on simulated data to predict layer thickness. Optical contrast measurements serve as an independent reference for validating the PACBED-based thickness determination in the few-layer regime. For samples ranging from monolayers up to three layers, both methods demonstrate exact agreement, and up to 10 layers, deviations were not exceeding a single layer. In the further extended thickness range of up to approximately 50 layers, consistent results are obtained, with deviations of no more than two layers between methods. These results confirm that PACBED, combined with either DVM or CNN-based evaluation, offers a reliable, accurate, and non-destructive method for quantifying the thickness of 2D inorganic materials in transmission electron microscopy.</div></div>","PeriodicalId":18501,"journal":{"name":"Micron","volume":"201 ","pages":"Article 103963"},"PeriodicalIF":2.2,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145620127","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Quantitative reconstruction of the refractive index in photosensitive materials via DIC phase imaging 基于DIC相位成像的光敏材料折射率定量重建。

IF 2.2 3区工程技术

Micron Pub Date : 2026-02-01 Epub Date: 2025-11-15 DOI: 10.1016/j.micron.2025.103959

Chen Ye , Guangbiao Wang , Zizheng Cao , Yan Zhou , Jianjun Yu

{"title":"Quantitative reconstruction of the refractive index in photosensitive materials via DIC phase imaging","authors":"Chen Ye , Guangbiao Wang , Zizheng Cao , Yan Zhou , Jianjun Yu","doi":"10.1016/j.micron.2025.103959","DOIUrl":"10.1016/j.micron.2025.103959","url":null,"abstract":"<div><div>Photosensitive materials are widely used in micro-/nanofabrication and photonic device development, where their refractive index (RI) distributions play a critical role in determining optical performances. To enable precise measurements of the RI distribution in photosensitive materials — particularly in the microstructures of photoresists — this study proposes a quantitative reconstruction method based on differential interference contrast (DIC) microscopy. Unlike conventional approaches requiring intermediate phase retrieval or interferometric systems, the proposed method directly reconstructs RI from intensity images acquired at multiple polarization angles and axial planes. A global optimization framework based on the first Born approximation enables high spatial resolution, rapid computation (<span><math><mo>∼</mo></math></span>30 s for images of 250 <span><math><mo>×</mo></math></span> 250 pixels), and high measurement accuracy. Experimental validation on periodic photoresist patterns shows a clear RI contrast between bleached and unbleached regions, with absolute relative errors of the RI reconstruction below 0.4% and mean absolute relative error of 0.17%. This technique offers a compact and efficient solution for quantitative RI characterization, with promising applicability in micro-/nanofabrication, photonic device inspection, and process monitoring.</div></div>","PeriodicalId":18501,"journal":{"name":"Micron","volume":"201 ","pages":"Article 103959"},"PeriodicalIF":2.2,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145541400","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigation of electron beam resistance of mAzamiGreen using an electron photon hybrid microscope 用电子光子混合显微镜研究mazamiggreen的电子束电阻。

IF 2.2 3区工程技术

Micron Pub Date : 2026-02-01 Epub Date: 2025-10-28 DOI: 10.1016/j.micron.2025.103944

Mihiro Suzuki, Toshiki Shimizu, Hiroki Minoda

引用次数: 0

Morphological and phagocytosis involvement of hemocytes from the freshwater crab Barytelphusa cunicularis (Westwood in Sykes, 1836) 淡水蟹Barytelphusa culcularis血细胞的形态学和吞噬作用（Westwood in Sykes, 1836）

IF 2.2 3区工程技术

Micron Pub Date : 2026-02-01 Epub Date: 2025-11-04 DOI: 10.1016/j.micron.2025.103941

Sundararajan Sowmiya , Rangasamy Shanthi , Mouvery Athira , Mullaivanam Ramasamy Sivakumar

{"title":"Morphological and phagocytosis involvement of hemocytes from the freshwater crab Barytelphusa cunicularis (Westwood in Sykes, 1836)","authors":"Sundararajan Sowmiya , Rangasamy Shanthi , Mouvery Athira , Mullaivanam Ramasamy Sivakumar","doi":"10.1016/j.micron.2025.103941","DOIUrl":"10.1016/j.micron.2025.103941","url":null,"abstract":"<div><div>This study investigates the cellular immune responses of hemocytes against experimental targets (buffalo RBCs and yeast cells) in the freshwater crab <em>Barytelphusa cunicularis</em>. Total hemocyte count (THC), differential hemocyte count (DHC), viability of hemocytes using trypan blue exclusion test, inherent phagocytic ability and enhancement in phagocytic ability up on pre-treating with serum agglutinins was evaluated. The THC was determined to be 1.12 × 107 cells/ml. Further, transmission electron microscope (TEM) images showed three distinct cell types based on their morphological features and the presence of other cellular organelles. Additionally, the hemocyte count of <em>B. cunicularis</em> revealed 24 % hyaline cells with no granularity, 60 % semi-granular cells exhibiting variable cytoplasmic granularity and 16 % granular cells containing numerous large granules. Similarly, viability assays using trypan blue exclusion demonstrated a decline from 99 % to 92 % over a 120 min period. Moreover, the inherent phagocytic ability of hemocytes and enhancement in phagocytosis up on pre-treating with serum agglutinins was assessed using buffalo RBCs and yeast cells as targets. In addition, the inherent phagocytic ability was found to be 25 % and showed an enhancement to 39 % in buffalo RBCs for opsono-phagocytosis and for yeast cells it showed an enhancement from 26 % to 32 %. This study revealed the unusual occurrence of binucleate granulocyte devoid of nucleus. Understanding these cellular defense mechanisms contributes to elucidating the innate immunity of <em>B. cunicularis</em>.</div></div>","PeriodicalId":18501,"journal":{"name":"Micron","volume":"201 ","pages":"Article 103941"},"PeriodicalIF":2.2,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145475478","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

GeckoDrive-enabled STM for stable atomic resolution imaging in cryogen free superconducting magnet 支持geckodrive的STM在无冷超导磁体中实现稳定的原子分辨率成像。

IF 2.2 3区工程技术

Micron Pub Date : 2026-02-01 Epub Date: 2025-11-15 DOI: 10.1016/j.micron.2025.103958

Muhammad Touqeer , Syed Asad Maqbool , Behnam Esmaeilzadeh , Yubin Hou , Qingyou Lu

{"title":"GeckoDrive-enabled STM for stable atomic resolution imaging in cryogen free superconducting magnet","authors":"Muhammad Touqeer , Syed Asad Maqbool , Behnam Esmaeilzadeh , Yubin Hou , Qingyou Lu","doi":"10.1016/j.micron.2025.103958","DOIUrl":"10.1016/j.micron.2025.103958","url":null,"abstract":"<div><div>We present the design and performance of a novel scanning tunnelling microscope (STM) that demonstrate compatibility with a cryogen-free superconducting magnet operation. The STM features an isolated scanning unit housing a tightly clamped piezoelectric tube scanner (PTS) and a zirconia shaft collectively forming the PTS unit supported by a zirconia frame. To minimize magnetic field interference, all components within the tip-sample mechanical loop are made of sapphire and zirconia. The PTS unit are clamped firmly to suppress vibrations induced by the magnet. For coarse approach, we employ an improved GeckoDrive motor capable of delivering an output force exceeding 2.2 N to actuate the tightly held PTS unit. After tunneling current detection, the tip-sample mechanical loop is mechanically decoupled from the piezo motor to suppress vibration transfer, while high-voltage signal interference is mitigated through proper shielding and grounding, ensuring stable imaging. The devices exceptional performance was validated through atomic-resolution imaging of graphite. The system demonstrates excellent stability, maintaining low drift rates in both the X-Y plane and Z direction at room and cryogenic temperatures, ensuring precise tip-sample alignment. Furthermore, we obtained atomic-resolution images of HOPG by varying the magnetic field from 0 T to 9 T at 300 K and acquired <span><math><mrow><mtext>dI</mtext><mo>/</mo><mtext>dV</mtext></mrow></math></span> spectra of an NbSe₂ sample at 3 K. These results indicated that our compact device is highly suitable for STM imaging and STS spectroscopy studies in high magnetic fields and low temperatures. Its high output force and stable performance make it well-suited for exploring magnetic field effects and low-temperature electronic properties in quantum and strongly correlated materials.</div></div>","PeriodicalId":18501,"journal":{"name":"Micron","volume":"201 ","pages":"Article 103958"},"PeriodicalIF":2.2,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145557225","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Electron energy loss spectroscopy of oriented targets and magnetic transitions 定向目标和磁跃迁的电子能量损失谱。

IF 2.2 3区工程技术

Micron Pub Date : 2026-02-01 Epub Date: 2025-10-31 DOI: 10.1016/j.micron.2025.103929

Ioannis Iatrakis , Valerii Brudanin

引用次数: 0

High-throughput analysis of dislocation loops in irradiated metals using Mask R-CNN 利用掩膜R-CNN高通量分析辐照金属中的位错环。

IF 2.2 3区工程技术

Micron Pub Date : 2026-02-01 Epub Date: 2025-10-22 DOI: 10.1016/j.micron.2025.103927

Camilo A.F. Salvador, Thomas Bilyk, Antoine Dartois, Lisa Vessier–Alary, Estelle Meslin, Mihai-Cosmin Marinica

{"title":"High-throughput analysis of dislocation loops in irradiated metals using Mask R-CNN","authors":"Camilo A.F. Salvador, Thomas Bilyk, Antoine Dartois, Lisa Vessier–Alary, Estelle Meslin, Mihai-Cosmin Marinica","doi":"10.1016/j.micron.2025.103927","DOIUrl":"10.1016/j.micron.2025.103927","url":null,"abstract":"<div><div>Advances in transmission electron microscopy under extreme conditions have enabled in situ experiments to capture vast amounts of data on defect evolution. On the other hand, computer vision models such as Mask R-CNN have become popular in the last few years, enabling fast and accurate segmentation of images of different natures. In the present work, we propose a workflow to label, segment, and analyze irradiation-induced defects in TEM images using Mask R-CNN. The work focuses on interpreting bright-field (BF) videos recorded during the irradiation of three different metallic materials. After establishing a baseline dataset based on austenitic stainless steel 316L, we tested small and large models as the backbone of Mask R-CNN and different hyperparameters for training them. Our best model predicts the areal density of defects in 316L with 83.6 % accuracy, measured by a custom metric which assesses cumulative defect area using an ellipsoidal approximation. We tested the generalization limits of the trained model to ensure accurate estimations of key physical metrics, including the foreground fraction occupied by defects, the number of detected particles, and their relative sizes — all of which exhibit relative errors below 5%. At last, the model helps interpret videos concerning two similar irradiation experiments: one with the 16Cr-37Fe-13Mn-34Ni (at. %) alloy, and another with pure Cr. The model’s segmentation clearly captures the different nature of defect evolution between different materials, as expected. Moreover, the proposed workflow not only enables consistent, real-time analysis of small defect loops during in situ TEM experiments but also generates the quantitative data needed to refine mesoscale models.</div></div>","PeriodicalId":18501,"journal":{"name":"Micron","volume":"201 ","pages":"Article 103927"},"PeriodicalIF":2.2,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145409483","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

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