Memorias do Instituto Oswaldo Cruz最新文献_第5页

The present and future research agenda of sporotrichosis on the silver anniversary of zoonotic sporotrichosis in Rio de Janeiro, Brazil. 在巴西里约热内卢人畜共患病孢子丝菌病银周年之际，探讨孢子丝菌病目前和未来的研究议程。

IF 2.8 4区医学

Memorias do Instituto Oswaldo Cruz Pub Date : 2024-02-12 eCollection Date: 2024-01-01 DOI: 10.1590/0074-02760230208

Rodrigo Almeida-Paes, Antonio Carlos Francesconi do Valle, Dayvison Francis Saraiva Freitas, Priscila Marques de Macedo, Rosely Maria Zancopé-Oliveira, Maria Clara Gutierrez-Galhardo

{"title":"The present and future research agenda of sporotrichosis on the silver anniversary of zoonotic sporotrichosis in Rio de Janeiro, Brazil.","authors":"Rodrigo Almeida-Paes, Antonio Carlos Francesconi do Valle, Dayvison Francis Saraiva Freitas, Priscila Marques de Macedo, Rosely Maria Zancopé-Oliveira, Maria Clara Gutierrez-Galhardo","doi":"10.1590/0074-02760230208","DOIUrl":"10.1590/0074-02760230208","url":null,"abstract":"Twenty-five years have passed since the initial observation of endemic zoonotic sporotrichosis in Rio de Janeiro, Brazil. Since then, this disease has spread throughout South America. Accompanying the emergence of this mycosis, some progress has been made, including the expansion of a research network in this field and higher visibility of sporotrichosis within government authorities and funding agencies. However, there are still some challenges to curbing the expansion of this disease in the coming years. These include the development of rapid and accurate diagnostic tests, new antifungal drugs, particularly for the treatment of extracutaneous manifestations of sporotrichosis, and more comprehensive care for cats with sporotrichosis. Including these actions in the sporotrichosis research agenda is required so as to change the development of this disease in the years to come.","PeriodicalId":18469,"journal":{"name":"Memorias do Instituto Oswaldo Cruz","volume":"119 ","pages":"e230208"},"PeriodicalIF":2.8,"publicationDate":"2024-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10868377/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139741420","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Simple and promising paper-based electrochemical platform for serological detection of American tegumentary leishmaniasis. 用于美洲利什曼病血清学检测的纸质电化学平台操作简单、前景广阔。

IF 2.8 4区医学

Memorias do Instituto Oswaldo Cruz Pub Date : 2024-02-12 eCollection Date: 2024-01-01 DOI: 10.1590/0074-02760230149

Daniela E Barraza, Paula I Nanni, María E Bracamonte, Roberto E Chaile, Carla B Goy, Leonardo Acuña, Jorge D Marco, Rossana E Madrid

{"title":"Simple and promising paper-based electrochemical platform for serological detection of American tegumentary leishmaniasis.","authors":"Daniela E Barraza, Paula I Nanni, María E Bracamonte, Roberto E Chaile, Carla B Goy, Leonardo Acuña, Jorge D Marco, Rossana E Madrid","doi":"10.1590/0074-02760230149","DOIUrl":"10.1590/0074-02760230149","url":null,"abstract":"Background: American tegumentary leishmaniasis (ATL) is an endemic neglected tropical disease (NTD), its conventional treatment is toxic, slow, and invasive. Rapid diagnosis is crucial for the clinical management of suspected patients, so the development and use of low-cost, miniaturised and portable devices could be the key.Objectives: This work aimed to develop a simple paper-based electrochemical platform for the serological detection of ATL.Methods: Platform was fabricated in Whatman N°1 paper, contains a hydrophobic zone generated by wax printing, two pencil graphite electrodes, and uses specific crude extracts (CA) antigens for ATL immuno-determination. The platform performance was analysed by measuring the relative impedance change for different antigen-antibody combinations. Then, 10 serum human samples previously diagnosed by the gold standard (five positive ATL cases and five non-ATL cases) were evaluated.Findings: The platform presented a linear response for the charge transfer resistance (ΔRct) and the interface reactance (ΔXc). Also, optimal working conditions were established (1/60 serum dilution and 180 µg/mL CA concentration). Then, the platform permits to distinguish between ATL and non-ATL (p < 0.05) human serum samples.Main conclusions: Our platform could allow the diagnosis, management, and monitoring of leishmaniasis while being an extremely simple and environmentally friendly technology.","PeriodicalId":18469,"journal":{"name":"Memorias do Instituto Oswaldo Cruz","volume":"119 ","pages":"e230149"},"PeriodicalIF":2.8,"publicationDate":"2024-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10868376/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139741419","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterisation of an area of coexistent visceral and cutaneous leishmaniasis transmission in the State of Piauí, Brazil. 巴西皮奥伊州内脏利什曼病和皮肤利什曼病共存地区的特征。

IF 2.8 4区医学

Memorias do Instituto Oswaldo Cruz Pub Date : 2024-02-05 eCollection Date: 2024-01-01 DOI: 10.1590/0074-02760230181

Silvia Alcântara Vasconcelos, Raimundo Leoberto Torres de Sousa, Enéas Costa Junior, João Paulo Diniz E Souza, Diane Cavalcante, Antônio Carlos Lima da Silva, Ivete Lopes de Mendonça, Jacenir Mallet, Clarissa Romero Teixeira, Guilherme Loureiro Werneck, Thais Araújo-Pereira, Daniela Pita-Pereira, Constança Britto, Maurício Luiz Vilela, Regis Gomes

{"title":"Characterisation of an area of coexistent visceral and cutaneous leishmaniasis transmission in the State of Piauí, Brazil.","authors":"Silvia Alcântara Vasconcelos, Raimundo Leoberto Torres de Sousa, Enéas Costa Junior, João Paulo Diniz E Souza, Diane Cavalcante, Antônio Carlos Lima da Silva, Ivete Lopes de Mendonça, Jacenir Mallet, Clarissa Romero Teixeira, Guilherme Loureiro Werneck, Thais Araújo-Pereira, Daniela Pita-Pereira, Constança Britto, Maurício Luiz Vilela, Regis Gomes","doi":"10.1590/0074-02760230181","DOIUrl":"10.1590/0074-02760230181","url":null,"abstract":"Background: In Brazil, transmission of visceral and cutaneous leishmaniasis has expanded geographically over the last decades, with both clinical forms occurring simultaneously in the same area.Objectives: This study characterised the clinical, spatial, and temporal distribution, and performed entomological surveillance and natural infection analysis of a leishmaniasis-endemic area.Methods: In order to characterise the risk of leishmaniasis transmission in Altos, Piauí, we described the clinical and socio-demographic variables and the spatial and temporal distribution of cases of American visceral leishmaniasis (AVL) and American cutaneous leishmaniasis (ACL) cases and identified potential phlebotomine vectors.Findings: The urban area concentrated almost 54% of ACL and 86.8% of AVL cases. The temporal and spatial distribution of AVL and ACL cases in Altos show a reduction in the number of risk areas, but the presence of permanent disease transmission foci is observed especially in the urban area. 3,808 phlebotomine specimens were captured, with Lutzomyia longipalpis as the most frequent species (98.45%). Of the 35 females assessed for natural infection, one specimen of Lu. longipalpis tested positive for the presence of Leishmania infantum and Leishmania braziliensis DNA.Main conclusion: Our results indicate the presence of risk areas for ACL and AVL in the municipality of Altos and highlight the importance of entomological surveillance to further understand a possible role of Lu. longipalpis in ACL transmission.","PeriodicalId":18469,"journal":{"name":"Memorias do Instituto Oswaldo Cruz","volume":"119 ","pages":"e230181"},"PeriodicalIF":2.8,"publicationDate":"2024-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10841424/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139702890","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The absence of eosinophils is associated with early metastatic lesions in Leishmania amazonensis-infected mice. 嗜酸性粒细胞的缺失与亚马逊利什曼原虫感染小鼠的早期转移性病变有关。

IF 2.8 4区医学

Memorias do Instituto Oswaldo Cruz Pub Date : 2024-01-08 eCollection Date: 2024-01-01 DOI: 10.1590/0074-02760220242

Gregório Guilherme Almeida, Tassiane Assíria Martins Luehring, Pierre Henrique de Menezes Paixão, Rodrigo Pedro Soares, André Luís Branco de Barros, Rubens Lima do Monte-Neto, Wagner Luiz Tafuri, Deborah Aparecida Negrão-Corrêa, Ricardo Gonçalves

{"title":"The absence of eosinophils is associated with early metastatic lesions in Leishmania amazonensis-infected mice.","authors":"Gregório Guilherme Almeida, Tassiane Assíria Martins Luehring, Pierre Henrique de Menezes Paixão, Rodrigo Pedro Soares, André Luís Branco de Barros, Rubens Lima do Monte-Neto, Wagner Luiz Tafuri, Deborah Aparecida Negrão-Corrêa, Ricardo Gonçalves","doi":"10.1590/0074-02760220242","DOIUrl":"10.1590/0074-02760220242","url":null,"abstract":"Background: Eosinophils are granulocytes that rapidly increase frequency in the bloodstream during helminthic infections and allergic responses. They are found in tissue infected by Leishmania during early disease, but their role during infection is not entirely understood.Objectives: We aim to compare the disease due to Leishmania amazonensis in BALB/c and Δdbl-GATA1 mice, which lack eosinophils.Methods: BALB/c and Δdbl-GATA1 mice infected with L. amazonensis were observed for several weeks. The parasite load and dissemination pattern were assessed.Findings: The Δdbl-GATA1 mice developed an anticipated dissemination of L. amazonensis and a worsening disease. No differences were found in the lesion development or the parasite load in the footpad among Δdbl-GATA1 mice and BALB/c eight weeks after infection. However, nine weeks after infection, massive growth of metastatic lesions appeared in several parts of the skin in Δdbl-GATA1 mice, weeks earlier than BALB/c. We observed increased parasites in the bloodstream, probably an essential dissemination route. Thirteen weeks after infection, metastatic lesions were found in all Δdbl-GATA1 mice.Main conclusion: These results suggest a protective role of eosinophils in delaying the disease caused by L. amazonensis, although several limitations of this mice strain must be considered.","PeriodicalId":18469,"journal":{"name":"Memorias do Instituto Oswaldo Cruz","volume":"119 ","pages":"e220242"},"PeriodicalIF":2.8,"publicationDate":"2024-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10777375/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139417386","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Histone deacetylase (HDAC) inhibitors- based drugs are effective to control Mycobacterium tuberculosis infection and promote the sensibility for rifampicin in MDR strain. 以组蛋白去乙酰化酶（HDAC）抑制剂为基础的药物可有效控制结核分枝杆菌感染，并提高耐药菌株对利福平的敏感性。

IF 2.8 4区医学

Memorias do Instituto Oswaldo Cruz Pub Date : 2023-12-22 eCollection Date: 2023-01-01 DOI: 10.1590/0074-02760230143

Adrián Rodríguez-Carlos, Yolanda Jacobo-Delgado, Alan Orlando Santos-Mena, Mariana H García-Hernández, Luis Adrian De Jesus-Gonzalez, Edgar E Lara-Ramirez, Bruno Rivas-Santiago

{"title":"Histone deacetylase (HDAC) inhibitors- based drugs are effective to control Mycobacterium tuberculosis infection and promote the sensibility for rifampicin in MDR strain.","authors":"Adrián Rodríguez-Carlos, Yolanda Jacobo-Delgado, Alan Orlando Santos-Mena, Mariana H García-Hernández, Luis Adrian De Jesus-Gonzalez, Edgar E Lara-Ramirez, Bruno Rivas-Santiago","doi":"10.1590/0074-02760230143","DOIUrl":"10.1590/0074-02760230143","url":null,"abstract":"Background: Tuberculosis (TB) is a major public health problem, which has been aggravated by the alarming growth of drug-resistant tuberculosis. Therefore, the development of a safer and more effective treatment is needed.Objectives: The aim of this work was repositioning and evaluate histone deacetylases (HDAC) inhibitors- based drugs with potential antimycobacterial activity.Methods: Using an in silico pharmacological repositioning strategy, three molecules that bind to the catalytic site of histone deacetylase were selected. Pneumocytes type II and macrophages were infected with Mycobacterium tuberculosis and treated with pre-selected HDAC inhibitors (HDACi). Subsequently, the ability of each of these molecules to directly promote the elimination of M. tuberculosis was evaluated by colony-forming unit (CFU)/mL. We assessed the expression of antimicrobial peptides and respiratory burst using reverse transcription-quantitative polymerase chain reaction (RT-qPCR).Findings: Aminoacetanilide (ACE), N-Boc-1,2-phenylenediamine (N-BOC), 1,3-Diphenylurea (DFU), reduce bacillary loads in macrophages and increase the production of β-defensin-2, LL-37, superoxide dismutase (SOD) 3 and inducible nitric oxide synthase (iNOS). While only the use of ACE in type II pneumocytes decreases the bacterial load through increasing LL-37 expression. Furthermore, the use of ACE and rifampicin inhibited the survival of intracellular multi-drug resistance M. tuberculosis.Main conclusions: Our data support the usefulness of in silico approaches for drug repositioning to provide a potential adjunctive therapy for TB.","PeriodicalId":18469,"journal":{"name":"Memorias do Instituto Oswaldo Cruz","volume":"118 ","pages":"e230143"},"PeriodicalIF":2.8,"publicationDate":"2023-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10740574/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138830409","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Predictors of Trypanosoma cruzi PCR positivity in patients with chronic Chagas disease. 慢性恰加斯病患者体内克氏锥虫 PCR 阳性的预测因素。

IF 2.8 4区医学

Memorias do Instituto Oswaldo Cruz Pub Date : 2023-12-15 eCollection Date: 2023-01-01 DOI: 10.1590/0074-02760230115

Ana Carolina Bastos de Lima, Veronica Gonçalves Mendes, Roberto Rodrigues Ferreira, Lindice Mitie Nisimura, Samuel Iwao Maia Horita, Henrique H Veloso, Andréa R Costa, Gilberto Marcelo S da Silva, Luiz Henrique C Sangenis, Marcelo T Holanda, Lorena Rimolo, Ademir B Cunha, Luciana Ribeiro Garzoni, Alejandro Marcel Hasslocher-Moreno, Mauro Felippe F Mediano, Otacílio da Cruz Moreira, Constança Britto, Roberto M Saraiva

{"title":"Predictors of Trypanosoma cruzi PCR positivity in patients with chronic Chagas disease.","authors":"Ana Carolina Bastos de Lima, Veronica Gonçalves Mendes, Roberto Rodrigues Ferreira, Lindice Mitie Nisimura, Samuel Iwao Maia Horita, Henrique H Veloso, Andréa R Costa, Gilberto Marcelo S da Silva, Luiz Henrique C Sangenis, Marcelo T Holanda, Lorena Rimolo, Ademir B Cunha, Luciana Ribeiro Garzoni, Alejandro Marcel Hasslocher-Moreno, Mauro Felippe F Mediano, Otacílio da Cruz Moreira, Constança Britto, Roberto M Saraiva","doi":"10.1590/0074-02760230115","DOIUrl":"10.1590/0074-02760230115","url":null,"abstract":"Background: A positive Trypanosoma cruzi polymerase chain reaction (PCR) is associated with a worse prognosis in patients with chronic Chagas disease (CD).Objectives: To study the association of clinical, electrocardiographic, and echocardiographic characteristics and biomarker blood levels with positive T. cruzi PCR in chronic CD.Methods: This is a single-centre observational cross-sectional study. Positive T. cruzi PCR association with clinical, electrocardiographic, and echocardiographic characteristics, and biomarker blood levels were studied by logistic regression analysis. p values < 0.05 were considered significant.Findings: Among 333 patients with chronic CD (56.4% men; 62 ± 10 years), T. cruzi PCR was positive in 41.1%. Stepwise multivariate logistic regression showed an independent association between positive T. cruzi PCR and diabetes mellitus {odds ratio (OR) 0.53 [95% confidence interval (CI) 0.30-0.93]; p = 0.03}, right bundle branch block [OR 1.78 (95% CI 1.09-2.89); p = 0.02], and history of trypanocidal treatment [OR 0.13 (95% CI 0.04-0.38); p = 0.0002]. Among patients with a history of trypanocidal treatment (n = 39), only four (10%) patients had a positive T. cruzi PCR.Main conclusions: Among several studied parameters, only diabetes mellitus, right bundle branch block, and history of trypanocidal treatment showed an independent association with positive T. cruzi PCR. History of trypanocidal treatment was a strong protective factor against a positive T. cruzi PCR.","PeriodicalId":18469,"journal":{"name":"Memorias do Instituto Oswaldo Cruz","volume":"118 ","pages":"e230115"},"PeriodicalIF":2.8,"publicationDate":"2023-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10727046/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138830410","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antileishmanial effects of γCdcPLI, a phospholipase A2 inhibitor from Crotalus durissus collilineatus snake serum, on Leishmania (Leishmania) amazonensis. 从Crotalus durissus collilineatus蛇血清中提取的磷脂酶A2抑制剂γCdcPLI对亚马孙利什曼原虫的抗利什曼原虫作用。

IF 2.8 4区医学

Memorias do Instituto Oswaldo Cruz Pub Date : 2023-11-27 eCollection Date: 2023-01-01 DOI: 10.1590/0074-02760220225

Marina Neves Gonçalves, Daiana Silva Lopes, Samuel Cota Teixeira, Thaise Lara Teixeira, Vitor de Freitas, Tássia Rafaella Costa, Sarah Natalie Cirilo Gimenes, Isabella Mitie de Camargo, Guilherme de Souza, Marcelo Santos da Silva, Fernanda Van Petten de Vasconcelos Azevedo, Kathleen Fernandes Grego, Luísa Carregosa Santos, Vinícius Queiroz Oliveira, Claudio Vieira da Silva, Renata Santos Rodrigues, Kelly Aparecida Geraldo Yoneyama, Patricia Bianca Clissa, Veridiana de Melo Rodrigues

{"title":"Antileishmanial effects of γCdcPLI, a phospholipase A2 inhibitor from Crotalus durissus collilineatus snake serum, on Leishmania (Leishmania) amazonensis.","authors":"Marina Neves Gonçalves, Daiana Silva Lopes, Samuel Cota Teixeira, Thaise Lara Teixeira, Vitor de Freitas, Tássia Rafaella Costa, Sarah Natalie Cirilo Gimenes, Isabella Mitie de Camargo, Guilherme de Souza, Marcelo Santos da Silva, Fernanda Van Petten de Vasconcelos Azevedo, Kathleen Fernandes Grego, Luísa Carregosa Santos, Vinícius Queiroz Oliveira, Claudio Vieira da Silva, Renata Santos Rodrigues, Kelly Aparecida Geraldo Yoneyama, Patricia Bianca Clissa, Veridiana de Melo Rodrigues","doi":"10.1590/0074-02760220225","DOIUrl":"10.1590/0074-02760220225","url":null,"abstract":"Background: Leishmaniasis, a neglected disease caused by the parasite Leishmania, is treated with drugs associated with high toxicity and limited efficacy, in addition to constant reports of the emergence of resistant parasites. In this context, snake serums emerge as good candidates since they are natural sources with the potential to yield novel drugs.Objectives: We aimed to show the antileishmanial effects of γCdcPLI, a phospholipase A2 inhibitor from Crotalus durissus collilineatus snake serum, against Leishmania (Leishmania) amazonensis.Methods: Promastigotes forms were exposed to γCdcPLI, and we assessed the parasite viability and cell cycle, as well as invasion and proliferation assays.Findings: Despite the low cytotoxicity effect on macrophages, our data indicate that γCdcPLI has a direct effect on parasites promoting an arrest in the G1 phase and reduction in the G2/M phase at the highest dose tested. Moreover, this PLA2 inhibitor reduced the parasite infectivity when promastigotes were pre-treated. Also, we demonstrated that the γCdcPLI treatment modulated the host cell environment impairing early and late steps of the parasitism.Main conclusions: γCdcPLI is an interesting tool for the discovery of new essential targets on the parasite, as well as an alternative compound to improve the effectiveness of the leishmaniasis treatment.","PeriodicalId":18469,"journal":{"name":"Memorias do Instituto Oswaldo Cruz","volume":"118 ","pages":"e220225"},"PeriodicalIF":2.8,"publicationDate":"2023-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10690931/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138451879","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unique synapomorphies and high diversity in South American Raji-related Epstein-Barr virus genomes. 南美Raji相关Epstein-Barr病毒基因组中独特的突触形态和高度多样性。

IF 2.8 4区医学

Memorias do Instituto Oswaldo Cruz Pub Date : 2023-11-03 eCollection Date: 2023-01-01 DOI: 10.1590/0074-02760230122

Paula Alves, Vanessa Emmel, Gustavo Stefanoff, Flavia Krsticevic, Joaquín Ezpeleta, Javier Murillo, Elizabeth Tapia, Edson Delatorre, Eliana Abdelhay, Rocio Hassan

{"title":"Unique synapomorphies and high diversity in South American Raji-related Epstein-Barr virus genomes.","authors":"Paula Alves, Vanessa Emmel, Gustavo Stefanoff, Flavia Krsticevic, Joaquín Ezpeleta, Javier Murillo, Elizabeth Tapia, Edson Delatorre, Eliana Abdelhay, Rocio Hassan","doi":"10.1590/0074-02760230122","DOIUrl":"10.1590/0074-02760230122","url":null,"abstract":"Background: Epstein-Barr virus (EBV) is a human gammaherpesvirus etiologically linked to several benign and malignant diseases. EBV-associated malignancies exhibit an unusual global distribution that might be partly attributed to virus and host genetic backgrounds.Objectives: To assemble a new genome of EBV (CEMO3) from a paediatric Burkitt's lymphoma from Rio de Janeiro State (Southeast Brazil). In addition, to perform global phylogenetic analysis using complete EBV genomes, including CEMO3, and investigate the genetic relationship of some South American (SA) genomes through EBV subgenomic targets.Methods: CEMO3 was sequenced through next generation sequencing and its coverage and gaps were corrected through the Sanger method. CEMO3 and 67 EBV genomes representing diverse geographic regions were evaluated through maximum likelihood phylogenetic analysis. Further, the polymorphism of subgenomic regions of some SA EBV genomes were assessed.Findings: The whole bulk tumour sequencing yielded 23,217 reads related to EBV, which 172,713 base pairs of the newly EBV genome CEMO3 was assembled. The CEMO3 and most SA EBV genomes clustered within the SA subclade closely related to the African Raji strain, forming the South American/Raji clade. Notably, these Raji-related genomes exhibit significant genetic diversity, characterised by distinctive synapomorphies at some gene levels absent in the original Raji strain.Conclusion: The CEMO3 represents a new South American EBV genome assembled. Albeit the majority of EBV genomes from SA are Raji-related, it harbours a high diversity different from the original Raji strain.","PeriodicalId":18469,"journal":{"name":"Memorias do Instituto Oswaldo Cruz","volume":"118 ","pages":"e230122"},"PeriodicalIF":2.8,"publicationDate":"2023-11-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10629697/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71483359","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unveiling the genome of a high-risk pandrug-resistant Klebsiella pneumoniae emerging in the Brazilian Amazon Region, 2022. 2022年，公布巴西亚马逊地区出现的高风险耐泛药肺炎克雷伯菌的基因组。

IF 2.8 4区医学

Memorias do Instituto Oswaldo Cruz Pub Date : 2023-10-27 eCollection Date: 2023-01-01 DOI: 10.1590/0074-02760230081

Érica Lourenço Fonseca, Sérgio M Morgado, Fernanda S Freitas, Nathalia S Bighi, Rosângela Cipriano, Ana Carolina Paulo Vicente

{"title":"Unveiling the genome of a high-risk pandrug-resistant Klebsiella pneumoniae emerging in the Brazilian Amazon Region, 2022.","authors":"Érica Lourenço Fonseca, Sérgio M Morgado, Fernanda S Freitas, Nathalia S Bighi, Rosângela Cipriano, Ana Carolina Paulo Vicente","doi":"10.1590/0074-02760230081","DOIUrl":"10.1590/0074-02760230081","url":null,"abstract":"Background: Pandrug-resistant (PDR) Klebsiella pneumoniae has been reported sporadically in many countries and remains rare in Brazil.Objectives: This study unravelled the genetic determinants involved with the PDR background of a clinical ST11 K. pneumoniae recovered in the Brazilian Amazon Region, where K. pneumoniae genomic and epidemiological information is scarce.Methods: Kp196 was submitted to the antimicrobial susceptibility test by the disk-diffusion method and minimum inhibitory concentration (MIC) determination. The whole genome sequencing was obtained and the sequence type was determined by core genome multilocus sequence typing (cgMLST). Its intrinsic and acquired resistome was assessed by Comprehensive Antibiotic Resistance Database (CARD) and comparison with wild-type genes.Findings: The analyses revealed that Kp196 belonged to the pandemic ST11 and presented the PDR phenotype. Its acquired resistome was composed of a huge set of clinically relevant resistance determinants, including bla CTX-M-15 and bla NDM-1, all found in the vicinity of mobile platforms. Considering its intrinsic resistome, the multidrug resistance, especially to colistin, tigecycline and fluoroquinolones, was multifactorial and attributed to modifications (indels, missense mutations, and gene disruption) in several housekeeping genes (arnT/phoQ/mgrB/ramR/acrB/gyrA/parC/ompK35-36-37). The Kp196 intrinsic resistome was also observed in a ST11 environmental strain, although harbouring distinct acquired resistomes.Conclusions: An accumulation of different resistance mechanisms regarding the intrinsic resistome accounts for a more stable resistome, strongly contributing to the Kp196 PDR phenotype.","PeriodicalId":18469,"journal":{"name":"Memorias do Instituto Oswaldo Cruz","volume":"118 ","pages":"e230081"},"PeriodicalIF":2.8,"publicationDate":"2023-10-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10626633/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71424881","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Benznidazole treatment decreases IL-6 levels in Trypanosoma cruzi-infected human adipocytes differentiated from adipose tissue-derived stem cells. 苯硝唑治疗降低了从脂肪组织衍生的干细胞分化的感染克氏锥虫的人类脂肪细胞中的IL-6水平。

IF 2.8 4区医学

Memorias do Instituto Oswaldo Cruz Pub Date : 2023-10-23 eCollection Date: 2023-01-01 DOI: 10.1590/0074-02760220295

Leyllane Rafael Moreira, Ana Carla Silva, Cíntia Nascimento da Costa Oliveira, Claudeir Dias da Silva Júnior, Amanda Vasconcelos Nascimento, Kamila Kássia Dos Santos Oliveira, Ana Karine de Araújo Soares, Karina Lidianne Alcântara Saraiva, Milena de Paiva Cavalcanti, Virginia Maria Barros de Lorena

{"title":"Benznidazole treatment decreases IL-6 levels in Trypanosoma cruzi-infected human adipocytes differentiated from adipose tissue-derived stem cells.","authors":"Leyllane Rafael Moreira, Ana Carla Silva, Cíntia Nascimento da Costa Oliveira, Claudeir Dias da Silva Júnior, Amanda Vasconcelos Nascimento, Kamila Kássia Dos Santos Oliveira, Ana Karine de Araújo Soares, Karina Lidianne Alcântara Saraiva, Milena de Paiva Cavalcanti, Virginia Maria Barros de Lorena","doi":"10.1590/0074-02760220295","DOIUrl":"10.1590/0074-02760220295","url":null,"abstract":"Background: Trypanosoma cruzi, which causes Chagas disease (CD), is a versatile haemoparasite that uses several strategies to evade the host's immune response, including adipose tissue (AT), used as a reservoir of infection. As it is an effective barrier to parasite evasion, the effectiveness of the drug recommended for treating CD, Benznidazole (BZ), may be questionable.Objective: To this end, we evaluated the parasite load and immunomodulation caused by BZ treatment in the culture of adipocytes differentiated from human adipose tissue-derived stem cells (ADSC) infected with T. cruzi.Methods: The ADSC were subjected to adipogenic differentiation. We then carried out four cultures in which we infected the differentiated AT with trypomastigote forms of the Y strain of T. cruzi and treated them with BZ. After the incubation, the infected AT was subjected to quantitative polymerase chain reaction (qPCR) to quantify the parasite load and transmission electron microscopy (TEM) to verify the infection. The supernatant was collected to measure cytokines, chemokines, and adipokines.Findings: We found elevated secretion of IL-6, CXCL-10/IP-10, CCL2/MCP-1, CCL5/RANTES, and leptin in infected fat cells. However, treatment with BZ promoted a decrease in IL-6.Main conclusion: Therefore, we believe that BZ has a beneficial role as it reduces inflammation in infected fat cells.","PeriodicalId":18469,"journal":{"name":"Memorias do Instituto Oswaldo Cruz","volume":"118 ","pages":"e220295"},"PeriodicalIF":2.8,"publicationDate":"2023-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10599316/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50162134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0