Memorias do Instituto Oswaldo Cruz最新文献

{"title":"First comparative genomics analysis of Corynebacterium auriscanis.","authors":"Ana Lua de Oliveira Vinhal, Max Roberto Batista de Araújo, Evandro Bento Rodrigues, Diogo Luiz de Carvalho Castro, Carine Rodrigues Pereira, Dircéia Aparecida Costa Custódio, Elaine Maria Seles Dorneles, Flávia Figueira Aburjaile, Bertram Brenig, Vasco Azevedo, Marcus Vinicius Canário Viana","doi":"10.1590/0074-02760240156","DOIUrl":"10.1590/0074-02760240156","url":null,"abstract":"<p><strong>Background: </strong>Corynebacterium auriscanis is a bacterial species frequently isolated from dogs with external otitis or dermatitis and a zoonotic pathogen transmitted by dog bite. It is considered an opportunistic pathogen, but its pathogenicity mechanisms are poorly studied. Comparative genomics can identify virulence and niche factors that could contribute to understanding its lifestyle.</p><p><strong>Objectives: </strong>The objectives of this project was to compare genomes of C. auriscanis to identify genes related to its virulence and lifestyle.</p><p><strong>Methods: </strong>The genome of strain 32 was sequenced using Illumina HiSeq 2500 (Illumina, CA, USA) and assembled using Unicycler. The two other non-redundant genomes from the same species available in GenBank were included in the analysis. All genomes were annotated and checked for taxonomy, assembly quality, mobile elements, CRISPR-Cas systems, and virulence and antimicrobial resistance genes. The virulence genes in the three genomes were compared to the ones from other pathogens commonly isolated with C. auriscanis.</p><p><strong>Findings: </strong>The species has 42 virulence factors that can be classified as niche factors, due to the absence of true virulence factors found in primary pathogens. The gene rbpA could confer basal levels of resistance to rifampin.</p><p><strong>Main conclusions: </strong>The absence of true virulence factors in the three genomes suggests C. auriscanis has an opportunistic pathogen lifestyle.</p>","PeriodicalId":18469,"journal":{"name":"Memorias do Instituto Oswaldo Cruz","volume":"119 ","pages":"e240156"},"PeriodicalIF":2.5,"publicationDate":"2024-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11508509/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142546245","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Detached epithelial cell plugs from the upper respiratory tract favour distal lung injury in Golden Syrian hamsters (Mesocricetus auratus) when experimentally infected with the A.2 Brazilian SARS-CoV-2 strain.","authors":"Marcelo Pelajo-Machado, Alexandre Dos Santos da Silva, Daniela Del Rosario Flores Rodrigues, Milla Bezerra Paiva, Rodrigo Muller, Luciana Jesus da Costa, Pedro Paulo Abreu Manso, João Paulo Rodrigues Dos Santos, Emanuelle de Souza Ramalho Ferreira da Silva, Arthur Daniel Rocha Alves, Jaqueline Mendes Oliveira, Marcelo Alves Pinto","doi":"10.1590/0074-02760240100","DOIUrl":"https://doi.org/10.1590/0074-02760240100","url":null,"abstract":"<p><strong>Background: </strong>The Golden Syrian hamster (Mesocricetus auratus), Ferrets (Mustela putorius furo), and macaques have been described as useful laboratory animals naturally susceptible to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection.</p><p><strong>Objectives: </strong>To study the mechanism of lung injury, we describe the histopathological features of SARS-CoV-2 infection in Golden Syrian hamsters inoculated intranasally with the A.2 Brazilian strain.</p><p><strong>Methods: </strong>Hamsters were intranasally inoculated with the A.2 variant and euthanised at 3-, 5-, 10- and 15-days post-inoculation. The physical examination and body weight were recorded daily. Neutralising antibodies and viral RNA load of the respiratory tract were assessed during necropsies.</p><p><strong>Findings: </strong>The coronavirus disease 2019 (COVID-19) model presented body weight loss, high levels of respiratory viral RNA load, severe segmentary pneumonitis, and bronchial fistula besides lymphatic trapping and infiltration, like the human SARS-COV-2 pathogenesis. The presence of subepithelial lymphoeosinophilic infiltrate was highlighted in our results; it contributed to the detachment of SARS-CoV-2 nucleocapsid-positive epithelial cells resulting in the infectious cell plugs.</p><p><strong>Main conclusions: </strong>The SARS-CoV-2 caused segmentary pneumonia and vascular damage. In our comprehension, the infectious cell plugs, as being aspirated from the upper respiratory tract into the terminal bronchial lumen, work as a \"Trojan horse\", thus contributing to the dissemination of the SARS-CoV-2 infection into specific regions of the deep lung parenchyma.</p>","PeriodicalId":18469,"journal":{"name":"Memorias do Instituto Oswaldo Cruz","volume":"119 ","pages":"e240100"},"PeriodicalIF":2.5,"publicationDate":"2024-10-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11493349/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142503496","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First concrete documentation for presence of Aedes (Stegomyia) albopictus in Bolivia: dispelling previous anecdotes.","authors":"Frédéric Lardeux, Philippe Boussès, Rosenka Tejerina-Lardeux, Audric Berger, Christian Barnabé, Lineth Garcia","doi":"10.1590/0074-02760240063","DOIUrl":"https://doi.org/10.1590/0074-02760240063","url":null,"abstract":"<p><strong>Background: </strong>The presence of Aedes albopictus in Bolivia has been a subject of controversy, with a lack of concrete documentation.</p><p><strong>Objectives: </strong>This study aimed to provide evidence of Ae. albopictus presence in Bolivia.</p><p><strong>Methods: </strong>Larval habitats were sampled in Rosario del Yata and San Agustín, Guayaramerín Municipality, Beni Department, northern Bolivia. Collected mosquito larvae were reared to the L4 and adult stages for morphological identification, with some specimens sequenced for confirmation.</p><p><strong>Findings: </strong>Aedes albopictus was identified in multiple larval habitats within peridomestic areas, such as buckets, canisters, and cut plastic bottles used as flower vases in both localities, confirming its establishment in the area. This represents the first concrete documentation of the species in Bolivia. The collections (larvae and adults) have been deposited in the Medical Entomology Laboratory of the Universidad Mayor de San Simón in Cochabamba, Bolivia, and the Laboratory of Entomology of the Instituto Nacional de Laboratorios de Salud of the Ministry of Health in La Paz, Bolivia.</p><p><strong>Main conclusion: </strong>Given its role as a vector for arboviruses such as dengue and Chikungunya, Ae. albopictus should be incorporated into the Bolivian National Programme of Vector Control for monitoring.</p>","PeriodicalId":18469,"journal":{"name":"Memorias do Instituto Oswaldo Cruz","volume":"119 ","pages":"e240063"},"PeriodicalIF":2.5,"publicationDate":"2024-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11486332/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142469627","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rapid on-site detection of echinococcosis and schistosomiasis based on RPA.","authors":"Lvbo Tian, Ying Shi, Yu Yang, Yuchen Wang","doi":"10.1590/0074-02760230244","DOIUrl":"https://doi.org/10.1590/0074-02760230244","url":null,"abstract":"<p><strong>Background: </strong>Echinococcosis and schistosomiasis, caused by parasitic worms, pose significant threats to millions of people in the world. Rapid and effective pathogen detection and epidemic control by public health authorities are urgently needed.</p><p><strong>Objectives: </strong>In this study, we aimed to develop rapid on-site detection method to detect echinococcosis and schistosomiasis.</p><p><strong>Methods: </strong>Recombinase polymerase amplification (RPA) was utilised to examine its efficacy of detection of echinococcosis and schistosomiasis.</p><p><strong>Findings: </strong>The detection probes for RPA were created through comparing parasitic genomes from international genomic data and the sequences generated by our group. We established an optimised RPA on-site testing platform, which significantly reduces the detection time (less than 30 min) and simplifies the operation (free of expensive equipment) as compared to traditional polymerase chain reaction (PCR) method.</p><p><strong>Main conclusions: </strong>This RPA detection platform in our study for identifying echinococcosis or schistosomiasis pathogens would be greatly applicable for epidemic investigation, border screening, and early clinical diagnosis.</p>","PeriodicalId":18469,"journal":{"name":"Memorias do Instituto Oswaldo Cruz","volume":"119 ","pages":"e230244"},"PeriodicalIF":2.5,"publicationDate":"2024-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11469317/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142469628","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of larval habitats of Anopheles (Nyssorhynchus) darlingi and associated species in malaria areas in western Brazilian Amazon.","authors":"Fátima Dos Santos, Mingrui Xu, Lelys Bravo de Guenni, Ricardo Lourenço-de-Oliveira, Yasmin Rubio-Palis","doi":"10.1590/0074-02760240116","DOIUrl":"10.1590/0074-02760240116","url":null,"abstract":"<p><strong>Background: </strong>Anopheles darlingi is the most efficient vector of malaria parasites in the Neotropics. Nevertheless, the specificities of its larval habitats are still poorly known.</p><p><strong>Objectives: </strong>Characterize permanent larval habitats, and population dynamics of An. darlingi and other potential vectors in relation to climate, physicochemical variables, insect fauna and malaria cases.</p><p><strong>Methods: </strong>A 14-month longitudinal study was conducted in Porto Velho, Rondônia, western Brazilian Amazon. Monthly, 21 permanent water bodies were sampled. Immature anophelines and associated fauna were collected, physicochemical characteristics, and climate variables were recorded and analyzed.</p><p><strong>Findings: </strong>Five types of habitats were identified: lagoon, stream, stream combined with lagoon, stream combined with dam, and fishpond. A total of 60,927 anophelines were collected. The most abundant species in all habitats were Anopheles braziliensis and An. darlingi. The highest density was found in the lagoon, while streams had the highest species richness. Abundance was higher during the transition period wet-dry season. There was a lag of respectively four and five months between the peak of rainfall and the Madeira River level and the highest abundance of An. darlingi larvae, which were positively correlated with habitats partially shaded, pH close to neutrality, increase dissolved oxygen and sulphates.</p><p><strong>Main conclusions: </strong>The present study provides data on key factors defining permanent larval habitats for the surveillance of An. darlingi and other potential vectors as well as a log-linear Negative Binomial model based on immature mosquito abundance and climate variables to predict the increase in the number of malaria cases.</p>","PeriodicalId":18469,"journal":{"name":"Memorias do Instituto Oswaldo Cruz","volume":"119 ","pages":"e240116"},"PeriodicalIF":2.5,"publicationDate":"2024-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11458182/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142391702","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advances in the development of new vaccines for tuberculosis and Brazil's role in the effort forward the end TB strategy.","authors":"Ana Paula Junqueira-Kipnis, Luciana Cesar de Cerqueira Leite, Júlio Croda, Erica Chimara, Anna Cristina C Carvalho, Ricardo Alexandre Arcêncio","doi":"10.1590/0074-02760240093","DOIUrl":"10.1590/0074-02760240093","url":null,"abstract":"<p><p>Tuberculosis (TB) continues to be the world's leading killer of infectious diseases. Despite global efforts to gradually reduce the number of annual deaths and the incidence of this disease, the coronavirus disease 19 (COVID-19) pandemic caused decreased in TB detection and affected the prompt treatment TB which led to a setback to the 2019 rates. However, the development and testing of new TB vaccines has not stopped and now presents the possibility of implanting in the next five years a new vaccine that is affordable and might be used in the various key vulnerable populations affected by TB. Then, this assay aimed to discuss the main vaccines developed against TB that shortly could be selected and used worldwide, and additionally, evidence the Brazilian potential candidates' vaccines in developing in Brazil that could be considered among those in level advanced to TB end.</p>","PeriodicalId":18469,"journal":{"name":"Memorias do Instituto Oswaldo Cruz","volume":"119 ","pages":"e240093"},"PeriodicalIF":2.5,"publicationDate":"2024-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11452070/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142391701","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Point-of-care testing for COVID-19: a simple two-step molecular diagnostic development and validation during the SARS-CoV-2 pandemic.","authors":"Andre Akira Gonzaga Yoshikawa, Sabrina Fernandes Cardoso, Lívia Budziarek Eslabão, Iara Carolini Pinheiro, Priscila Valverde, Gisele Caminha, Oscar Bruna Romero, Leandro Medeiros, Luísa Damazio Pitaluga Rona, André Nóbrega Pitaluga","doi":"10.1590/0074-02760230236","DOIUrl":"10.1590/0074-02760230236","url":null,"abstract":"<p><strong>Background: </strong>During the coronavirus disease 19 (COVID-19) pandemic, diagnostic testing of the general population proved challenging due to limitations of the gold-standard diagnostic procedure using reverse transcription real-time polymerase chain reaction (RT-qPCR) for large-scale testing on the centralised model, especially in low-resource areas.</p><p><strong>Objectives: </strong>To address this, a point-of-care (PoC) diagnostic protocol for COVID-19 was developed, providing fast, reliable, and affordable testing, particularly for low-mid develop areas.</p><p><strong>Methods: </strong>The PoC diagnostic process combines a simple paper-based RNA extraction method housed within a 3D-printed plastic device with a colorimetric reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay. Nasopharyngeal/oropharyngeal swabs (NOS) and saliva samples were tested between 2020 and 2021, with the assistance of Santa Catarina's State Health Secretary, Brazil.</p><p><strong>Findings: </strong>The developed diagnostic protocol showed a limit of detection of 9,900 copies and an overall diagnostic specificity of 98% for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) from 1,348 clinical analysed samples. The diagnostic sensitivity was 95% for NOS samples, 85% for early morning saliva, and 69% for indiscriminate saliva.</p><p><strong>Main conclusions: </strong>In conclusion, the developed device successfully extracted SARS-CoV-2 viral RNA from swabs and saliva clinical samples. When combined with colorimetric RT-LAMP, it provides results within 45 min using minimal resources, thus delivering a diagnostic kit protocol that is applicable in large-scale sampling.</p>","PeriodicalId":18469,"journal":{"name":"Memorias do Instituto Oswaldo Cruz","volume":"119 ","pages":"e230236"},"PeriodicalIF":2.5,"publicationDate":"2024-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11452069/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142391713","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Overview of extracellular vesicles in pathogens with special focus on human extracellular protozoan parasites.","authors":"Johan Alvarado-Ocampo, Elizabeth Abrahams-Sandí, Lissette Retana-Moreira","doi":"10.1590/0074-02760240073","DOIUrl":"https://doi.org/10.1590/0074-02760240073","url":null,"abstract":"<p><p>Extracellular vesicles (EVs) are lipid-bilayered membrane-delimited particles secreted by almost any cell type, involved in different functions according to the cell of origin and its state. From these, cell to cell communication, pathogen-host interactions and modulation of the immune response have been widely studied. Moreover, these vesicles could be employed for diagnostic and therapeutic purposes, including infections produced by pathogens of diverse types; regarding parasites, the secretion, characterisation, and roles of EVs have been studied in particular cases. Moreover, the heterogeneity of EVs presents challenges at every stage of studies, which motivates research in this area. In this review, we summarise some aspects related to the secretion and roles of EVs from several groups of pathogens, with special focus on the most recent research regarding EVs secreted by extracellular protozoan parasites.</p>","PeriodicalId":18469,"journal":{"name":"Memorias do Instituto Oswaldo Cruz","volume":"119 ","pages":"e240073"},"PeriodicalIF":2.5,"publicationDate":"2024-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11421424/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142349766","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Description of the new HIV-1 intersubtype B/C circulating recombinant form (CRF146_BC) detected in Brazil.","authors":"Rodrigo Cunha Oliveira, Darren Martin, Juliana Sacramento Mota de Souza, Luiz Carlos Júnior Alcântara, Monick Lindenmeyer Guimarães, Carlos Brites, Joana Paixão Monteiro-Cunha","doi":"10.1590/0074-02760230214","DOIUrl":"https://doi.org/10.1590/0074-02760230214","url":null,"abstract":"<p><strong>Background: </strong>The human immunodeficiency virus 1 (HIV-1) infections in Brazil are predominantly caused by two subtypes, B and C.</p><p><strong>Objectives: </strong>Here we present the characterisation of a novel HIV-1 recombinant form, indicating a new Brazilian CRF_BC, named CRF146_BC.</p><p><strong>Methods: </strong>RDP, JphMM and Simplot recombination tools were used to evaluate the mosaic pattern.</p><p><strong>Findings: </strong>In this work, we identified three HIV-1 nucleotide sequences previously classified as unique recombinant forms (URFs), plus one new partial genome sharing the same BC recombination pattern. The mosaic genome is almost entirely represented by the subtype C sequence, with a small subtype B recombination region in the pol gene, at the Integrase level. The phylogenetic analyses strongly indicate a common origin between the strains, which were isolated in Rio Grande do Sul, Rio de Janeiro and Bahia states.</p><p><strong>Main conclusions: </strong>Thus, the new HIV-1 CRF146_BC is circulating in three different Brazilian regions: South, Southeast and Northeast.</p>","PeriodicalId":18469,"journal":{"name":"Memorias do Instituto Oswaldo Cruz","volume":"119 ","pages":"e230214"},"PeriodicalIF":2.5,"publicationDate":"2024-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11421422/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142349765","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Climate change-induced degradation of expert range maps drawn for kissing bugs (Hemiptera: Reduviidae) and long-standing current and future sampling gaps across the Americas.","authors":"Vaughn Shirey, Jorge Rabinovich","doi":"10.1590/0074-02760230100","DOIUrl":"https://doi.org/10.1590/0074-02760230100","url":null,"abstract":"<p><strong>Background: </strong>Kissing bugs are the vectors of Trypanosoma cruzi, the etiological agent of Chagas disease (CD). Despite their epidemiological relevance, kissing bug species are under sampled in terms of their diversity and it is unclear what biases exist in available kissing bug data. Under climate change, range maps for kissing bugs may become less accurate as species shift their ranges to track climatic tolerance.</p><p><strong>Objectives: </strong>Quantify inventory completeness in available kissing bug data. Assess how well range maps are at conveying information about current distributions and potential future distributions subject to shift under climate change. Intersect forecasted changes in kissing bug distributions with contemporary sampling gaps to identify regions for future sampling of the group. Identify whether a phylogenetic signal is present in expert range knowledge as more closely related species may be similarly well or lesser understood.</p><p><strong>Methods: </strong>We used species distribution models (SDM), specifically constructed from Bayesian additive regression trees, with Bioclim variables, to forecast kissing bug distributions into 2100 and intersect these with current sampling gaps to identify priority regions for sampling. Expert range maps were assessed by the agreement between the expert map and SDM generated occurrence probability. We used classical hypothesis testing methods as well as tests of phylogenetic signal to meet our objectives.</p><p><strong>Findings: </strong>Expert range maps vary in their quality of depicting current kissing bug distributions. Most expert range maps decline in their ability to convey information about kissing bug occurrence over time, especially in under sampled areas. We found limited evidence for a phylogenetic signal in expert range map performance.</p><p><strong>Main conclusions: </strong>Expert range maps are not a perfect account of species distributions and may degrade in their ability to accurately convey distribution knowledge under future climates. We identify regions where future sampling of kissing bugs will be crucial for completing biodiversity inventories.</p>","PeriodicalId":18469,"journal":{"name":"Memorias do Instituto Oswaldo Cruz","volume":"119 ","pages":"e230100"},"PeriodicalIF":2.5,"publicationDate":"2024-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11421423/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142349764","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}