X-linked polymorphisms in TLR7 and TLR8 genes are associated with protection against Chikungunya fever.

IF 2.5 4区医学 Q2 PARASITOLOGY

Memorias do Instituto Oswaldo Cruz Pub Date : 2025-06-13 eCollection Date: 2025-01-01 DOI:10.1590/0074-02760230224

Wilker Jose Perez Gotay, Mariella Sousa Coêlho Maciel, Raphael de Oliveira Rodrigues, Cynthia Chester Cardoso, Caroline Nobre Oliveira, Artur Fontenelle Lima Montenegro, Juliana Navarro Ueda Yaochite

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引用次数: 0

Abstract

Background: Chikungunya virus (CHIKV) causes an infection that leads to the activation of the innate immune response, triggering receptor pathways such as toll-like receptors (TLRs).

Objective: The present study aimed to investigate the association of single nucleotide polymorphisms (SNPs) in genes encoding toll-like receptors 3, 7, and 8 and IRF5 in susceptibility to CHIKV infection and persistent joint pain.

Methods: A case-control study was carried out. The study included 121 symptomatic cases, 29 asymptomatic cases, and 182 healthy controls matched for age and sex. Polymorphisms were identified by TaqMan® SNP Genotyping assays.

Findings: The G allele of the TLR7 variant (rs3853839 G/C) and the G allele of TLR8 (rs3764879 G/C) were associated with protection against CHIKV infection [adjusted odd ratio (OR) = 0.64; p = 0.02 and adjusted OR = 0.54; p = 0.001, respectively]. Moreover, individuals who presented the G allele in the rs3764879 variant have a greater chance of developing the asymptomatic form (adjusted OR =2.88; p =0.004). The development of persistent joint pain was not associated with any investigated SNPs in positive anti-CHIKV IgG individuals.

Main conclusions: This study identified TLR7 and TLR8 gene polymorphisms as protective factors for Chikungunya infection.

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TLR7和TLR8基因的x连锁多态性与基孔肯雅热保护有关。

背景：基孔肯雅病毒（CHIKV）引起感染，导致先天免疫反应激活，触发toll样受体（TLRs）等受体途径。目的：本研究旨在探讨toll样受体3、7、8和IRF5编码基因的单核苷酸多态性（snp）与CHIKV感染易感性和持续性关节疼痛的关系。方法：采用病例-对照研究。该研究包括121例有症状病例，29例无症状病例和182例年龄和性别匹配的健康对照。通过TaqMan®SNP基因分型分析鉴定多态性。结果：TLR7变异的G等位基因（rs3853839 G/C）和TLR8变异的G等位基因（rs3764879 G/C）与预防CHIKV感染相关[校正奇比(OR) = 0.64；p = 0.02，调整后OR = 0.54；P = 0.001]。此外，在rs3764879变异中携带G等位基因的个体发展为无症状形式的机会更大(调整OR =2.88；p = 0.004)。在抗chikv IgG阳性个体中，持续关节疼痛的发展与任何研究的snp无关。主要结论：TLR7和TLR8基因多态性是基孔肯雅热感染的保护因子。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Memorias do Instituto Oswaldo Cruz 医学-寄生虫学

CiteScore

5.00

自引率

3.60%

发文量

审稿时长

3-8 weeks

期刊介绍： Memórias do Instituto Oswaldo Cruz is a journal specialized in microbes & their vectors causing human infections. This means that we accept manuscripts covering multidisciplinary approaches and findings in the basic aspects of infectious diseases, e.g. basic in research in prokariotes, eukaryotes, and/or virus. Articles must clearly show what is the main question to be answered, the hypothesis raised, and the contribution given by the study. Priority is given to manuscripts reporting novel mechanisms and general findings concerning the biology of human infectious prokariotes, eukariotes or virus. Papers reporting innovative methods for diagnostics or that advance the basic research with these infectious agents are also welcome. It is important to mention what we do not publish: veterinary infectious agents research, taxonomic analysis and re-description of species, epidemiological studies or surveys or case reports and data re-analysis. Manuscripts that fall in these cases or that are considered of low priority by the journal editorial board, will be returned to the author(s) for submission to another journal.