Methods in cell biology最新文献_第3页

Development of an in vivo ovarian cancer peritoneal carcinomatosis model for radioimmunotherapy testing. 用于放射免疫治疗试验的卵巢癌腹膜癌模型的建立。

4区生物学

Methods in cell biology Pub Date : 2025-01-01 Epub Date: 2024-06-17 DOI: 10.1016/bs.mcb.2024.05.013

Clara Diaz Garcia-Prada, Salima Atis, Jean-Pierre Pouget, Julie Constanzo

{"title":"Development of an in vivo ovarian cancer peritoneal carcinomatosis model for radioimmunotherapy testing.","authors":"Clara Diaz Garcia-Prada, Salima Atis, Jean-Pierre Pouget, Julie Constanzo","doi":"10.1016/bs.mcb.2024.05.013","DOIUrl":"10.1016/bs.mcb.2024.05.013","url":null,"abstract":"Currently, Ovarian Cancer (OC) is the most lethal gynecological malignancy. In most patients, it progresses without clinical signs or symptoms, leading to a late diagnosis when it has already spread in the peritoneal cavity as peritoneal carcinomatosis (PC). To date, OC PC management is based on cytoreductive surgery to remove the macroscopic disease, followed by chemotherapy. Many patients respond to this treatment, but disease recurs in 70-90% of them. Therefore, new therapeutic approaches are needed. The field of targeted radionuclide therapy (TRT) has witnessed considerable progress and several radiopharmaceuticals have been approved in the last decade. In TRT, radiolabeled molecules are injected to specifically recognize, irradiate, and kill tumor cells. TRT is a multisite radiotherapy that delivers dose to all malignant lesions. Therefore, TRT could be an alternative approach for OC PC because conventional external beam radiotherapy cannot be used at curative dose due to toxicity to healthy tissues. Here, we describe an OC PC model based on grafting human SK-OV-3 OC cells in the peritoneal cavity of immunodeficient mice. We also explain how to label trastuzumab with lutetium-177 to specifically target and irradiate SK-OV-3 cell nodules in these mice, and how to monitor the response to this TRT in vivo. With minor variations, the same technique can be conveniently applied to a variety of human (or mouse) tumors.","PeriodicalId":18437,"journal":{"name":"Methods in cell biology","volume":"192 ","pages":"131-157"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143039848","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The current models unravel the molecular mechanisms underlying the intricate pathophysiology of Alzheimer's disease using zebrafish. 目前的模型揭示了斑马鱼阿尔茨海默病复杂病理生理的分子机制。

4区生物学

Methods in cell biology Pub Date : 2025-01-01 Epub Date: 2024-04-18 DOI: 10.1016/bs.mcb.2024.03.009

Baban Thawkar, Ginpreet Kaur

{"title":"The current models unravel the molecular mechanisms underlying the intricate pathophysiology of Alzheimer's disease using zebrafish.","authors":"Baban Thawkar, Ginpreet Kaur","doi":"10.1016/bs.mcb.2024.03.009","DOIUrl":"10.1016/bs.mcb.2024.03.009","url":null,"abstract":"The foremost cause of dementia is Alzheimer's disease (AD). The vital pathological hallmarks of AD are amyloid beta (Aβ) peptide and hyperphosphorylated tau (p-tau) protein. The current animal models used in AD research do not precisely replicate disease pathophysiology, making it difficult for researchers to quickly and effectively gather data or screen potential therapy possibilities. Several transgenic animals are used as models for AD; however, they have cost and time concerns. Zebrafish (Danio rerio) has become a suitable model organism for high-throughput pharmacological screening of neuroactive substances and neurodegenerative research. The past few decades have seen a significant increase in research on AD. The fight against amyloidosis has, however, been unexpectedly unsuccessful. It may be due to a need for more relevant in vivo models for high throughput screening, which emphasizes the need to find other anti-AD models. Alternative animal models, including zebrafish, have developed into a potentially useful research tool that must be employed for AD research to be effective. Only a few comprehensive zebrafish models exhibiting AD-like pathogenesis have been reported in the literature, and this book chapter describes these models.","PeriodicalId":18437,"journal":{"name":"Methods in cell biology","volume":"192 ","pages":"17-31"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143039913","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Methodology and evaluation of the induction of experimental autoimmune encephalomyelitis, a murine preclinical model of multiple sclerosis. 实验性自身免疫性脑脊髓炎（多发性硬化症小鼠临床前模型）的诱导方法和评价。

4区生物学

Methods in cell biology Pub Date : 2025-01-01 Epub Date: 2025-05-31 DOI: 10.1016/bs.mcb.2025.03.024

Hugo Calvo, Mónica Macías, Inmaculada Simón, María Isabel Cabero, Yolanda Álvarez, Juliana Carvalho-Tavares, Marita Hernández, María Luisa Nieto

{"title":"Methodology and evaluation of the induction of experimental autoimmune encephalomyelitis, a murine preclinical model of multiple sclerosis.","authors":"Hugo Calvo, Mónica Macías, Inmaculada Simón, María Isabel Cabero, Yolanda Álvarez, Juliana Carvalho-Tavares, Marita Hernández, María Luisa Nieto","doi":"10.1016/bs.mcb.2025.03.024","DOIUrl":"https://doi.org/10.1016/bs.mcb.2025.03.024","url":null,"abstract":"Multiple sclerosis (MS) is a chronic inflammatory disease of the central nervous system (CNS) that is characterized by a severe and progressive demyelinating process. It is considered a neurodegenerative autoimmune disorder driven by immune cell infiltration, overproduction of cytokines and reactive oxygen species (ROS) accumulation that leads to axonal and neuronal injury. Experimental autoimmune encephalomyelitis (EAE) is the most commonly used pre-clinical model of multiple sclerosis (MS), since it resembles many aspects of the human disease. EAE can be induced in a variety of species and strains (rodents and monkeys), providing models of acute monophasic, relapsing-remitting and chronic progressive CNS inflammation. Thus, the pathology of the lesions varies according to the animal model used. We herein describe in detail a protocol for induction of EAE in C57BL/6 mice by immunization with MOG35-55 in CFA, which induces a monophasic, chronic and sustained form of EAE. In addition, we also describe approaches to evaluate disease induction and a technique for pathological examination of CNS tissues to assess ROS accumulation. This animal model could be useful for acute and chronic studies and to assess the effectiveness of different treatments.","PeriodicalId":18437,"journal":{"name":"Methods in cell biology","volume":"197 ","pages":"109-120"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145033790","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Combined in vitro differentiation and cell sorting-based isolation of highly pure mouse bone marrow-derived basophils. 结合体外分化和细胞分选分离高纯度小鼠骨髓源性嗜碱性粒细胞。

4区生物学

Methods in cell biology Pub Date : 2025-01-01 Epub Date: 2025-02-09 DOI: 10.1016/bs.mcb.2025.01.009

Adriana Rosa Gambardella, Valentina Tirelli, Sara Andreone, Jacopo Mancini, Fabrizio Mattei, Giovanna Schiavoni

{"title":"Combined in vitro differentiation and cell sorting-based isolation of highly pure mouse bone marrow-derived basophils.","authors":"Adriana Rosa Gambardella, Valentina Tirelli, Sara Andreone, Jacopo Mancini, Fabrizio Mattei, Giovanna Schiavoni","doi":"10.1016/bs.mcb.2025.01.009","DOIUrl":"https://doi.org/10.1016/bs.mcb.2025.01.009","url":null,"abstract":"Basophils constitute a rare population of granulocytes with key functions in allergies, immunodeficiencies and cancer. The scarcity of basophils in human blood and tissues constitutes a considerable limit for the study of these cells. Interleukin-3 (IL-3) stimulates both the differentiation and the expansion of basophils from bone marrow (BM) precursors by positively regulating the expression of the IL3Ra receptor. We have standardized an in vitro differentiation protocol of mouse basophils (mBaso) from BM precursors through culture in presence of IL-3 for 10 days followed by cell sorting. At the end of the 10-day differentiation, a considerable number of mBaso can be obtained and cell sorting procedures further improved the isolation of an extraordinarily pure (>98 %) and vital FcεR1+ CD11c- c-kit- mBaso population. Phenotypic analysis of terminally differentiated (day 10) unsorted mBaso cultured for 24 h showed a decrease in basophilic lineage (c-kit-) and an increase of mastocytic lineage (c-kit+) and reduced the expression of basophil markers FcεRI, CD49b and CD200R either in absence of stimuli or following activation with the alarmin IL-33, indicating cell dedifferentiation. In contrast, terminally differentiated and FcεR1+ CD11c- c-kit- sorted mBaso do not dedifferentiate in mast cells when placed in culture, and responded to IL-33 stimulation by up-regulating the activation marker CD63 without down-modulation of FcεRI and CD200R3. These evidences highlight that in vitro differentiation followed by cell sorting is a useful method to obtain elevated numbers of highly pure mBaso that preserve their lineage markers and thus are suitable for conducting the desired functional studies.","PeriodicalId":18437,"journal":{"name":"Methods in cell biology","volume":"196 ","pages":"193-208"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144667963","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cannulation of mesenteric lymphatic vessels in a murine model. 小鼠模型肠系膜淋巴管插管。

4区生物学

Methods in cell biology Pub Date : 2025-01-01 Epub Date: 2025-05-27 DOI: 10.1016/bs.mcb.2025.05.001

Zohaib Nisar Khan

引用次数: 0

Assessing mitochondrial number and morphology in a C. elegans model of human tauopathy. 评估秀丽隐杆线虫模型人类tau病的线粒体数量和形态。

4区生物学

Methods in cell biology Pub Date : 2025-01-01 Epub Date: 2025-05-29 DOI: 10.1016/bs.mcb.2025.05.002

Eleni Tsakiri, Giorgos Niforos-Garcia, Brian D Ackley, Konstantinos Palikaras

{"title":"Assessing mitochondrial number and morphology in a C. elegans model of human tauopathy.","authors":"Eleni Tsakiri, Giorgos Niforos-Garcia, Brian D Ackley, Konstantinos Palikaras","doi":"10.1016/bs.mcb.2025.05.002","DOIUrl":"https://doi.org/10.1016/bs.mcb.2025.05.002","url":null,"abstract":"Mitochondrial dysfunction is a shared hallmark of neurodegenerative disorders, including Alzheimer's disease (AD) and tauopathies among others. Pathological alterations of the microtubule-associated protein Tau can disrupt mitochondrial dynamics, transport, and function, ultimately leading to neuronal toxicity and synaptic deficits. Understanding these processes is crucial for developing therapeutic interventions. The nematode Caenorhabditis elegans serves as a powerful model to study mitochondrial morphology and Tau-induced neurotoxicity due to its well-characterized nervous system and genetic tractability. Here, we describe a robust methodology for assessing mitochondrial morphology, Tau aggregation, and neuronal integrity in a nematode model of tauopathy. By combining confocal laser scanning microscopy and motility assays, we provide a comprehensive framework for investigating mitochondrial deficits. This approach offers valuable insights into the interplay between Tau pathology and mitochondrial dysfunction, thereby advancing our understanding of neurodegenerative mechanisms and potential therapeutic targets.","PeriodicalId":18437,"journal":{"name":"Methods in cell biology","volume":"197 ","pages":"275-290"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145033770","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cell culture techniques for cancer research. 用于癌症研究的细胞培养技术。

4区生物学

Methods in cell biology Pub Date : 2025-01-01 Epub Date: 2025-03-18 DOI: 10.1016/bs.mcb.2025.02.023

Preeti Jain, Nitika Joshi, Sadhna Aggarwal

引用次数: 0

Liquid biopsy and circulating tumor cell analysis. 液体活检和循环肿瘤细胞分析。

4区生物学

Methods in cell biology Pub Date : 2025-01-01 Epub Date: 2025-03-11 DOI: 10.1016/bs.mcb.2025.02.019

Wasiur Rahman Choudhury, Rama Rao Damerla, K Devaraja

引用次数: 0

Ex vivo assessment of human neutrophil motility and migration. 人中性粒细胞运动和迁移的离体评估。

4区生物学

Methods in cell biology Pub Date : 2025-01-01 Epub Date: 2024-11-19 DOI: 10.1016/bs.mcb.2024.10.008

Noor A M Bakker, Claudia Burrello, Karin E de Visser

引用次数: 0

In vitro ILC differentiation from human HSCs. 人造血干细胞的体外ILC分化。

4区生物学

Methods in cell biology Pub Date : 2025-01-01 Epub Date: 2024-11-19 DOI: 10.1016/bs.mcb.2024.10.004

Silvia Santopolo, Cecilia Ciancaglini, Francesca Romana Mariotti, Lorenzo Moretta, Linda Quatrini

{"title":"In vitro ILC differentiation from human HSCs.","authors":"Silvia Santopolo, Cecilia Ciancaglini, Francesca Romana Mariotti, Lorenzo Moretta, Linda Quatrini","doi":"10.1016/bs.mcb.2024.10.004","DOIUrl":"10.1016/bs.mcb.2024.10.004","url":null,"abstract":"The Innate Lymphoid Cells (ILCs) are a family of innate immune cells composed by the Natural Killer (NK) cells and the helper ILCs (hILCs) (ILC1, ILC2, ILC3), both developing from a common ILC precursor (ILCP) derived from hematopoietic stem cells (HSCs). A correct ILC reconstitution is crucial, particularly in patients receiving HSC transplantation (HSCT), the only therapeutic option for many adult and pediatric high-risk hematological malignancies. Indeed, mainly thanks to their cytotoxic activity, NK cells have a strong Graft-versus-Leukemia (GvL) effect. On the other hand, hILCs, that are mainly tissue resident, are involved in tissue repair and homeostasis, Graft-versus-Host Disease (GvHD) prevention and immune response to infections. Unlike NK cell development, hILC-poiesis is still poorly characterized in humans. Here, we provide a protocol for the in vitro ILC differentiation from healthy donor peripheral blood-derived CD34+ HSCs. This could represent a useful model to dissect the molecular mechanisms by which the distinct ILC subsets are generated from ILCP leading to the development of novel strategies to improve the HSCT clinical outcome.","PeriodicalId":18437,"journal":{"name":"Methods in cell biology","volume":"191 ","pages":"41-57"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143008130","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0