Cell culture techniques for cancer research.

Cancer is perceived as difficult to treat due to its capacity to manifest in different forms and lack of knowledge of mechanical details. Advances in the analytical techniques relevant to boosting cancer research require an hour. With the help of cell culture techniques, several significant advances in cancer research have been made in the recent past. The main difficulty associated with cell culture techniques of cancer research is to create an in vivo tumor microenvironment cost-effectively. Here, in this chapter, we have discussed the different protocols for utilizing cell culture techniques in cancer research. The 2D, 3D, scaffold and organoid based cell culture techniques have been covered in detail. In addition, we have presented a comparative analysis, including advantages and disadvantages of each type of cell culture technique. Moreover, the assays, which can be used for assessing the quality of cancer cell lines, have been listed in detail.