Methods in cell biology最新文献_第10页

DNA damage and chromosomal instability. DNA 损伤和染色体不稳定性

4区生物学

Methods in cell biology Pub Date : 2024-01-01 DOI: 10.1016/S0091-679X(24)00029-3

Christian Zierhut

引用次数: 0

A new procedure to induce aortic aneurysms in mice. 诱发小鼠主动脉瘤的新程序。

4区生物学

Methods in cell biology Pub Date : 2024-01-01 Epub Date: 2024-05-31 DOI: 10.1016/bs.mcb.2024.03.005

Raquel Rodrigues-Díez, Antonio Tejera-Muñoz, Raúl R Rodrigues-Diez

{"title":"A new procedure to induce aortic aneurysms in mice.","authors":"Raquel Rodrigues-Díez, Antonio Tejera-Muñoz, Raúl R Rodrigues-Diez","doi":"10.1016/bs.mcb.2024.03.005","DOIUrl":"https://doi.org/10.1016/bs.mcb.2024.03.005","url":null,"abstract":"Aortic aneurysms (AAs) are a major public health challenge, featured by a progressive impairs in aortic wall integrity that drives to aortic dilation and, in end stage, to its rupture. Despite important advances in the surgical treatment of aortic aneurysms, there is currently no pharmacological intervention that prevents their development, reduces their expansion, or avoids their rupture. In addition to classic risk factors such age or gender, several heritable connective tissue disorders have been associated with AA developing, highlighting the role of extracellular matrix (ECM) genes alterations in the developing of AA. In this sense, we have recently demonstrated that global deletion of the cellular communicating network factor 2 (CCN2), previously known as connective tissue growth factor (CTGF) due to its role in the extracellular matrix formation, predisposes to early and lethal AAs development after Angiotensin II (Ang II) infusion in mice. Here, we detail the protocol to induce and detect AAs generation in inducible global CCN2 knockout mice after Ang II infusion which allow the characterization of CCN role in AA development and may help to the development of pharmacological target for AA treatment.","PeriodicalId":18437,"journal":{"name":"Methods in cell biology","volume":"188 ","pages":"61-71"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141331337","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Purification and analysis of kidney-infiltrating leukocytes in a mouse model of lupus nephritis. 狼疮肾炎小鼠模型中肾脏浸润白细胞的纯化与分析

4区生物学

Methods in cell biology Pub Date : 2024-01-01 Epub Date: 2024-05-01 DOI: 10.1016/bs.mcb.2024.03.007

Laura Amo, Hemanta K Kole, Bethany Scott, Francisco Borrego, Chen-Feng Qi, Hongsheng Wang, Silvia Bolland

{"title":"Purification and analysis of kidney-infiltrating leukocytes in a mouse model of lupus nephritis.","authors":"Laura Amo, Hemanta K Kole, Bethany Scott, Francisco Borrego, Chen-Feng Qi, Hongsheng Wang, Silvia Bolland","doi":"10.1016/bs.mcb.2024.03.007","DOIUrl":"https://doi.org/10.1016/bs.mcb.2024.03.007","url":null,"abstract":"Renal injury often occurs as a complication in autoimmune diseases such as systemic lupus erythematosus (SLE). It is estimated that a minimum of 20% SLE patients develop lupus nephritis, a condition that can be fatal when the pathology progresses to end-stage renal disease. Studies in animal models showed that incidence of immune cell infiltrates in the kidney was linked to pathological injury and correlated with severe lupus nephritis. Thus, preventing immune cell infiltration into the kidney is a potential approach to impede the progression to an end-stage disease. A requirement to investigate the role of kidney-infiltrating leukocytes is the development of reproducible and efficient protocols for purification and characterization of immune cells in kidney samples. This chapter describes a detailed methodology that discriminates tissue-resident leukocytes from blood-circulating cells that are found in kidney. Our protocol was designed to maximize cell viability and to reduce variability among samples, with a combination of intravascular staining and magnetic bead separation for leukocyte enrichment. Experiments included as example were performed with FcγRIIb[KO] mice, a well-characterized murine model of SLE. We identified T cells and macrophages as the primary leukocyte subsets infiltrating into the kidney during severe nephritis, and we extensively characterized them phenotypically by flow cytometry.","PeriodicalId":18437,"journal":{"name":"Methods in cell biology","volume":"188 ","pages":"131-152"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141331347","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Animal models of disease: Achievements and challenges. 疾病动物模型：成就与挑战。

4区生物学

Methods in cell biology Pub Date : 2024-01-01 DOI: 10.1016/S0091-679X(24)00164-X

José Manuel Bravo-San Pedro, Fernando Aranda, Aitziber Buqué, Lorenzo Galluzzi

引用次数: 0

A pharmacodynamic assay to monitor treatment with the hypomethylating cytosine analogs, decitabine and azacitidine. 用于监测低甲基化胞嘧啶类似物地西他滨和阿扎胞苷治疗的药效学试验。

4区生物学

Methods in cell biology Pub Date : 2024-01-01 Epub Date: 2024-03-21 DOI: 10.1016/bs.mcb.2024.02.016

James W Jacobberger, Philip G Woost

引用次数: 0

Spectral analysis and sorting of microbial organisms using a spectral sorter. 使用光谱分拣机对微生物进行光谱分析和分拣。

4区生物学

Methods in cell biology Pub Date : 2024-01-01 Epub Date: 2024-03-12 DOI: 10.1016/bs.mcb.2024.02.017

Sharath Narayana Iyengar, J Paul Robinson

引用次数: 0

Induction of chromosome-specific micronuclei and chromothripsis by centromere inactivation. 通过中心粒失活诱导染色体特异性微核和染色体三分裂。

4区生物学

Methods in cell biology Pub Date : 2024-01-01 Epub Date: 2022-11-28 DOI: 10.1016/bs.mcb.2022.10.009

Yu-Fen Lin, Qing Hu, Alison Guyer, Daniele Fachinetti, Peter Ly

{"title":"Induction of chromosome-specific micronuclei and chromothripsis by centromere inactivation.","authors":"Yu-Fen Lin, Qing Hu, Alison Guyer, Daniele Fachinetti, Peter Ly","doi":"10.1016/bs.mcb.2022.10.009","DOIUrl":"10.1016/bs.mcb.2022.10.009","url":null,"abstract":"Chromothripsis describes the catastrophic fragmentation of individual chromosomes followed by its haphazard reassembly into a derivative chromosome harboring complex rearrangements. This process can be initiated by mitotic cell division errors when one or more chromosomes aberrantly mis-segregate into micronuclei and acquire extensive DNA damage. Approaches to induce the formation of micronuclei encapsulating random chromosomes have been used; however, the eventual reincorporation of the micronucleated chromosome into daughter cell nuclei poses a challenge in tracking the chromosome for multiple cell cycles. Here we outline an approach to genetically engineer stable human cell lines capable of efficient chromosome-specific micronuclei induction. This strategy, which targets the CENP-B-deficient Y chromosome centromere for inactivation, allows the stepwise process of chromothripsis to be experimentally recapitulated, including the mechanisms and timing of chromosome fragmentation. Lastly, we describe the integration of a selection marker onto the micronucleated Y chromosome that enables the diverse genomic rearrangement landscape arising from micronuclei formation to be interrogated.","PeriodicalId":18437,"journal":{"name":"Methods in cell biology","volume":"182 ","pages":"1-20"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11008423/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139741406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Machine learning approach to assess brain metastatic burden in preclinical models. 在临床前模型中评估脑转移负荷的机器学习方法。

4区生物学

Methods in cell biology Pub Date : 2024-01-01 Epub Date: 2024-10-29 DOI: 10.1016/bs.mcb.2024.10.001

Jessica Rappaport, Quanyi Chen, Tomi McGuire, Amélie Daugherty-Lopès, Romina Goldszmid

引用次数: 0

Characterization of lysosomal acid lipase in Ly6G⁺ and CD11c⁺ myeloid-derived suppressor cells. Ly6G+和CD11c+髓源性抑制细胞溶酶体酸性脂肪酶的特征。

4区生物学

Methods in cell biology Pub Date : 2024-01-01 Epub Date: 2023-10-11 DOI: 10.1016/bs.mcb.2023.05.011

Ting Zhao, Hong Du, Cong Yan

引用次数: 0

Generation of glial cell-derived neurotrophic factor (gdnf) morphants in zebrafish larvae by cerebroventricular microinjection of vivo morpholino. 通过脑室显微注射 vivo morpholino 在斑马鱼幼体中生成胶质细胞源性神经营养因子 (gdnf) 形态体。

4区生物学

Methods in cell biology Pub Date : 2024-01-01 Epub Date: 2022-10-31 DOI: 10.1016/bs.mcb.2022.09.004

Suzita Mohd Noor, Chee Ern David Wong, Pooi-Fong Wong, Anwar Norazit

{"title":"Generation of glial cell-derived neurotrophic factor (gdnf) morphants in zebrafish larvae by cerebroventricular microinjection of vivo morpholino.","authors":"Suzita Mohd Noor, Chee Ern David Wong, Pooi-Fong Wong, Anwar Norazit","doi":"10.1016/bs.mcb.2022.09.004","DOIUrl":"10.1016/bs.mcb.2022.09.004","url":null,"abstract":"Dopaminergic neurons in the brain are an important source of dopamine, which is a crucial neurotransmitter for wellbeing, memory, reward, and motor control. Deficiency of dopamine due to advanced age and accumulative dopaminergic neuron defects can lead to movement disorders such as Parkinson's disease. Glial cell-derived neurotrophic factor (GDNF) is one of many factors involved in dopaminergic neuron development and/or survival. However, other endogenous GDNF functions in the brain await further investigation. Zebrafish is a well-established genetic model for neurodevelopment and neurodegeneration studies. Importantly, zebrafish shares approximately 70% functional orthologs with human genes including GDNF. To gain a better understanding on the precise functional role of gdnf in dopaminergic neurons, our laboratory devised a targeted knockdown of gdnf in the zebrafish larval brain using vivo morpholino. Here, detailed protocols on the generation of gdnf morphants using vivo morpholino are outlined. This method can be applied for targeting of genes in the brain to determine specific spatiotemporal gene function in situ.","PeriodicalId":18437,"journal":{"name":"Methods in cell biology","volume":"181 ","pages":"17-32"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139672172","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0