{"title":"Cell culture techniques for cancer research.","authors":"Preeti Jain, Nitika Joshi, Sadhna Aggarwal","doi":"10.1016/bs.mcb.2025.02.023","DOIUrl":null,"url":null,"abstract":"<p><p>Cancer is perceived as difficult to treat due to its capacity to manifest in different forms and lack of knowledge of mechanical details. Advances in the analytical techniques relevant to boosting cancer research require an hour. With the help of cell culture techniques, several significant advances in cancer research have been made in the recent past. The main difficulty associated with cell culture techniques of cancer research is to create an in vivo tumor microenvironment cost-effectively. Here, in this chapter, we have discussed the different protocols for utilizing cell culture techniques in cancer research. The 2D, 3D, scaffold and organoid based cell culture techniques have been covered in detail. In addition, we have presented a comparative analysis, including advantages and disadvantages of each type of cell culture technique. Moreover, the assays, which can be used for assessing the quality of cancer cell lines, have been listed in detail.</p>","PeriodicalId":18437,"journal":{"name":"Methods in cell biology","volume":"198 ","pages":"27-71"},"PeriodicalIF":0.0000,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Methods in cell biology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1016/bs.mcb.2025.02.023","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/3/18 0:00:00","PubModel":"Epub","JCR":"Q4","JCRName":"Biochemistry, Genetics and Molecular Biology","Score":null,"Total":0}
引用次数: 0
Abstract
Cancer is perceived as difficult to treat due to its capacity to manifest in different forms and lack of knowledge of mechanical details. Advances in the analytical techniques relevant to boosting cancer research require an hour. With the help of cell culture techniques, several significant advances in cancer research have been made in the recent past. The main difficulty associated with cell culture techniques of cancer research is to create an in vivo tumor microenvironment cost-effectively. Here, in this chapter, we have discussed the different protocols for utilizing cell culture techniques in cancer research. The 2D, 3D, scaffold and organoid based cell culture techniques have been covered in detail. In addition, we have presented a comparative analysis, including advantages and disadvantages of each type of cell culture technique. Moreover, the assays, which can be used for assessing the quality of cancer cell lines, have been listed in detail.
期刊介绍：
For over fifty years, Methods in Cell Biology has helped researchers answer the question "What method should I use to study this cell biology problem?" Edited by leaders in the field, each thematic volume provides proven, state-of-art techniques, along with relevant historical background and theory, to aid researchers in efficient design and effective implementation of experimental methodologies. Over its many years of publication, Methods in Cell Biology has built up a deep library of biological methods to study model developmental organisms, organelles and cell systems, as well as comprehensive coverage of microscopy and other analytical approaches.
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