Microbial Cell最新文献_第2页

Unresolved mystery of cyclic nucleotide second messengers, periplasmic acid phosphatases and bacterial natural competence. 环核苷酸第二信使、质周酸性磷酸酶和细菌自然能力的未解之谜。

IF 4.1 3区生物学

Microbial Cell Pub Date : 2024-07-18 eCollection Date: 2024-01-01 DOI: 10.15698/mic2024.07.828

Kristina Kronborg, Yong Everett Zhang

{"title":"Unresolved mystery of cyclic nucleotide second messengers, periplasmic acid phosphatases and bacterial natural competence.","authors":"Kristina Kronborg, Yong Everett Zhang","doi":"10.15698/mic2024.07.828","DOIUrl":"10.15698/mic2024.07.828","url":null,"abstract":"We recently characterized the competitive inhibition of cyclic AMP (cAMP) on three periplasmic acid phosphatases, AphAHi, NadNHi, and eP4 (HelHi), in Haemophilus influenzae Rd KW20. This inhibitory effect is vital for orchestrating the nutritional growth and competence development in KW20. Initially discovered in Escherichia coli, the function of AphA remains however obscure. This study investigates the regulation of E. coli aphA expression under nutrient starvation conditions. Using transcriptional reporters with truncated aphA promoter sequences, we found that starvations of carbon and phosphate, but not amino acid, stimulated aphA expression through distinct promoter regions. Deletions of crp or cyaA abolished aphA expression, confirming their crucial roles. Conversely, CytR deletion increased aphA expression, suggesting CytR's role as a repressor of aphA expression. Additionally, we extended the study of three other second messengers, i.e., cyclic GMP, cyclic UMP, and cyclic CMP, each sharing structural similarities with cAMP. Notably, cGMP competitively inhibits AphAHi's acid phosphatase activity akin to cAMP. In contrast, both cUMP and cCMP stimulate AphAHi's phosphatase activity in a concentration dependent manner. Collectively, these data imply a complicated connection between nucleotide metabolism, AphA, cyclic purine and pyrimidine nucleotides in bacterial nutrient uptake and natural competence.","PeriodicalId":18397,"journal":{"name":"Microbial Cell","volume":"11 ","pages":"235-241"},"PeriodicalIF":4.1,"publicationDate":"2024-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11261661/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141748547","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterising glycosaminoglycans in human breastmilk and their potential role in infant health. 人类母乳中糖胺聚糖的特征及其对婴儿健康的潜在作用。

IF 4.1 3区生物学

Microbial Cell Pub Date : 2024-07-04 eCollection Date: 2024-01-01 DOI: 10.15698/mic2024.07.827

Melissa Greenwood, Patricia Murciano-Martínez, Janet Berrington, Sabine L Flitsch, Sean Austin, Christopher Stewart

{"title":"Characterising glycosaminoglycans in human breastmilk and their potential role in infant health.","authors":"Melissa Greenwood, Patricia Murciano-Martínez, Janet Berrington, Sabine L Flitsch, Sean Austin, Christopher Stewart","doi":"10.15698/mic2024.07.827","DOIUrl":"10.15698/mic2024.07.827","url":null,"abstract":"Human breastmilk is composed of many well researched bioactive components crucial for infant nutrition and priming of the neonatal microbiome and immune system. Understanding these components gives us crucial insight to the health and wellbeing of infants. Research surrounding glycosaminoglycans (GAGs) previously focused on those produced endogenously; however, recent efforts have shifted to understanding GAGs in human breastmilk. The structural complexity of GAGs makes detection and analysis complicated therefore, research is time consuming and limited to highly specialised teams experienced in carbohydrate analysis. In breastmilk, GAGs are present in varying quantities in four forms; chondroitin sulphate, heparin/heparan sulphate, dermatan sulphate and hyaluronic acid, and are hypothesised to behave similar to other bioactive components with suspected roles in pathogen defense and proliferation of beneficial gut bacteria. Chondroitin sulphate and heparin, being the most abundant, are expected to have the most impact on infant health. Their decreasing concentration over lactation further indicates their role and potential importance during early life.","PeriodicalId":18397,"journal":{"name":"Microbial Cell","volume":"11 ","pages":"221-234"},"PeriodicalIF":4.1,"publicationDate":"2024-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11224681/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141556481","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Neutralizing the threat: harnessing broadly neutralizing antibodies against HIV-1 for treatment and prevention. 中和威胁：利用针对 HIV-1 的广泛中和抗体进行治疗和预防。

IF 4.1 3区生物学

Microbial Cell Pub Date : 2024-07-03 eCollection Date: 2024-01-01 DOI: 10.15698/mic2024.07.826

Juan C Becerra, Lauren Hitchcock, Khoa Vu, Johannes S Gach

{"title":"Neutralizing the threat: harnessing broadly neutralizing antibodies against HIV-1 for treatment and prevention.","authors":"Juan C Becerra, Lauren Hitchcock, Khoa Vu, Johannes S Gach","doi":"10.15698/mic2024.07.826","DOIUrl":"10.15698/mic2024.07.826","url":null,"abstract":"Broadly neutralizing antibodies (bnAbs) targeting the human immunodeficiency virus-1 (HIV-1) have played a crucial role in elucidating and characterizing neutralization-sensitive sites on the HIV-1 envelope spike and in informing vaccine development. Continual advancements in identifying more potent bnAbs, along with their capacity to trigger antibody-mediated effector functions, coupled with modifications to extend their half-life, position them as promising candidates for both HIV-1 treatment and prevention. While current pharmacological interventions have made significant progress in managing HIV-1 infection and enhancing quality of life, no definitive cure or vaccines have been developed thus far. Standard treatments involve daily oral anti-retroviral therapy, which, despite its efficacy, can lead to notable long-term side effects. Recent clinical trial data have demonstrated encouraging therapeutic and preventive potential for bnAb therapies in both HIV-1-infected individuals and those without the infection. This review provides an overview of the advancements in HIV-1-specific bnAbs and discusses the insights gathered from recent clinical trials regarding their application in treating and preventing HIV-1 infection.","PeriodicalId":18397,"journal":{"name":"Microbial Cell","volume":"11 ","pages":"207-220"},"PeriodicalIF":4.1,"publicationDate":"2024-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11224682/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141555216","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expansion of metabolically labelled endocytic organelles and cytoskeletal cell structures in Giardia lamblia using optimised U-ExM protocols. 使用优化的 U-ExM 方案扩增蓝氏贾第鞭毛虫体内的代谢标记内细胞器和细胞骨架结构。

IF 4.1 3区生物学

Microbial Cell Pub Date : 2024-06-21 eCollection Date: 2024-01-01 DOI: 10.15698/mic2024.06.825

Clirim Jetishi, Erina A Balmer, Bianca M Berger, Carmen Faso, Torsten Ochsenreiter

引用次数: 0

From microbes to medicine: harnessing the gut microbiota to combat prostate cancer. 从微生物到药物：利用肠道微生物群防治前列腺癌。

IF 4.6 3区生物学

Microbial Cell Pub Date : 2024-05-23 eCollection Date: 2024-01-01 DOI: 10.15698/mic2024.05.824

Anjali Yadav, Meenakshi Kaushik, Prabhakar Tiwari, Rima Dada

{"title":"From microbes to medicine: harnessing the gut microbiota to combat prostate cancer.","authors":"Anjali Yadav, Meenakshi Kaushik, Prabhakar Tiwari, Rima Dada","doi":"10.15698/mic2024.05.824","DOIUrl":"10.15698/mic2024.05.824","url":null,"abstract":"The gut microbiome (GM) has been identified as a crucial factor in the development and progression of various diseases, including cancer. In the case of prostate cancer, commensal bacteria and other microbes are found to be associated with its development. Recent studies have demonstrated that the human GM, including Bacteroides, Streptococcus, Bacteroides massiliensis, Faecalibacterium prausnitzii, Eubacterium rectale, and Mycoplasma genitalium, are involved in prostate cancer development through both direct and indirect interactions. However, the pathogenic mechanisms of these interactions are yet to be fully understood. Moreover, the microbiota influences systemic hormone levels and contributes to prostate cancer pathogenesis. Currently, it has been shown that supplementation of prebiotics or probiotics can modify the composition of GM and prevent the onset of prostate cancer. The microbiota can also affect drug metabolism and toxicity, which may improve the response to cancer treatment. The composition of the microbiome is crucial for therapeutic efficacy and a potential target for modulating treatment response. However, their clinical application is still limited. Additionally, GM-based cancer therapies face limitations due to the complexity and diversity of microbial composition, and the lack of standardized protocols for manipulating gut microbiota, such as optimal probiotic selection, treatment duration, and administration timing, hindering widespread use. Therefore, this review provides a comprehensive exploration of the GM's involvement in prostate cancer pathogenesis. We delve into the underlying mechanisms and discuss their potential implications for both therapeutic and diagnostic approaches in managing prostate cancer. Through this analysis, we offer valuable insights into the pivotal role of the microbiome in prostate cancer and its promising application in future clinical settings.","PeriodicalId":18397,"journal":{"name":"Microbial Cell","volume":"11 ","pages":"187-197"},"PeriodicalIF":4.6,"publicationDate":"2024-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11129862/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141158652","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Polyadenylated versions of small non-coding RNAs in Saccharomyces cerevisiae are degraded by Rrp6p/Rrp47p independent of the core nuclear exosome. 酿酒酵母中的多腺苷酸化小非编码 RNA 可由 Rrp6p/Rrp47p 降解，与核心核外泌体无关。

IF 4.6 3区生物学

Microbial Cell Pub Date : 2024-05-22 eCollection Date: 2024-01-01 DOI: 10.15698/mic2024.05.823

Anusha Chaudhuri, Soumita Paul, Mayukh Banerjea, Biswadip Das

{"title":"Polyadenylated versions of small non-coding RNAs in Saccharomyces cerevisiae are degraded by Rrp6p/Rrp47p independent of the core nuclear exosome.","authors":"Anusha Chaudhuri, Soumita Paul, Mayukh Banerjea, Biswadip Das","doi":"10.15698/mic2024.05.823","DOIUrl":"10.15698/mic2024.05.823","url":null,"abstract":"In Saccharomyces cerevisiae, polyadenylated forms of mature (and not precursor) small non-coding RNAs (sncRNAs) those fail to undergo proper 3'-end maturation are subject to an active degradation by Rrp6p and Rrp47p, which does not require the involvement of core exosome and TRAMP components. In agreement with this finding, Rrp6p/Rrp47p is demonstrated to exist as an exosome-independent complex, which preferentially associates with mature polyadenylated forms of these sncRNAs. Consistent with this observation, a C-terminally truncated version of Rrp6p (Rrp6p-ΔC2) lacking physical association with the core nuclear exosome supports their decay just like its full-length version. Polyadenylation is catalyzed by both the canonical and non-canonical poly(A) polymerases, Pap1p and Trf4p. Analysis of the polyadenylation profiles in WT and rrp6-Δ strains revealed that the majority of the polyadenylation sites correspond to either one to three nucleotides upstream or downstream of their mature ends and their poly(A) tails ranges from 10-15 adenylate residues. Most interestingly, the accumulated polyadenylated snRNAs are functional in the rrp6-Δ strain and are assembled into spliceosomes. Thus, Rrp6p-Rrp47p defines a core nuclear exosome-independent novel RNA turnover system in baker's yeast targeting imperfectly processed polyadenylated sncRNAs that accumulate in the absence of Rrp6p.","PeriodicalId":18397,"journal":{"name":"Microbial Cell","volume":"11 ","pages":"155-186"},"PeriodicalIF":4.6,"publicationDate":"2024-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11115967/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141087944","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring carbon source related localization and phosphorylation in the Snf1/Mig1 network using population and single cell-based approaches. 利用基于群体和单细胞的方法探索 Snf1/Mig1 网络中与碳源相关的定位和磷酸化。

IF 4.6 3区生物学

Microbial Cell Pub Date : 2024-05-16 eCollection Date: 2024-01-01 DOI: 10.15698/mic2024.05.822

Svenja Braam, Farida Tripodi, Linnea Österberg, Sebastian Persson, Niek Welkenhuysen, Paola Coccetti, Marija Cvijovic

{"title":"Exploring carbon source related localization and phosphorylation in the Snf1/Mig1 network using population and single cell-based approaches.","authors":"Svenja Braam, Farida Tripodi, Linnea Österberg, Sebastian Persson, Niek Welkenhuysen, Paola Coccetti, Marija Cvijovic","doi":"10.15698/mic2024.05.822","DOIUrl":"https://doi.org/10.15698/mic2024.05.822","url":null,"abstract":"The AMPK/SNF1 pathway governs energy balance in eukaryotic cells, notably influencing glucose de-repression. In S. cerevisiae, Snf1 is phosphorylated and hence activated upon glucose depletion. This activation is required but is not sufficient for mediating glucose de-repression, indicating further glucose-dependent regulation mechanisms. Employing fluorescence recovery after photobleaching (FRAP) in conjunction with non-linear mixed effects modelling, we explore the spatial dynamics of Snf1 as well as the relationship between Snf1 phosphorylation and its target Mig1 controlled by hexose sugars. Our results suggest that inactivation of Snf1 modulates Mig1 localization and that the kinetic of Snf1 localization to the nucleus is modulated by the presence of non-fermentable carbon sources. Our data offer insight into the true complexity of regulation of this central signaling pathway in orchestrating cellular responses to fluctuating environmental cues. These insights not only expand our understanding of glucose homeostasis but also pave the way for further studies evaluating the importance of Snf1 localization in relation to its phosphorylation state and regulation of downstream targets.","PeriodicalId":18397,"journal":{"name":"Microbial Cell","volume":"11 ","pages":"143-154"},"PeriodicalIF":4.6,"publicationDate":"2024-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11097897/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140958443","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Modular Cloning Toolkit for the production of recombinant proteins in Leishmania tarentolae. 用于生产重组蛋白的模块化克隆工具包。

IF 4.6 3区生物学

Microbial Cell Pub Date : 2024-04-30 eCollection Date: 2024-01-01 DOI: 10.15698/mic2024.04.821

Katrin Hieronimus, Tabea Donauer, Jonas Klein, Bastian Hinkel, Julia Vanessa Spänle, Anna Probst, Justus Niemeyer, Salina Kibrom, Anna Maria Kiefer, Luzia Schneider, Britta Husemann, Eileen Bischoff, Sophie Möhring, Nicolas Bayer, Dorothée Klein, Adrian Engels, Benjamin Gustav Ziehmer, Julian Stieβ, Pavlo Moroka, Michael Schroda, Marcel Deponte

{"title":"A Modular Cloning Toolkit for the production of recombinant proteins in Leishmania tarentolae.","authors":"Katrin Hieronimus, Tabea Donauer, Jonas Klein, Bastian Hinkel, Julia Vanessa Spänle, Anna Probst, Justus Niemeyer, Salina Kibrom, Anna Maria Kiefer, Luzia Schneider, Britta Husemann, Eileen Bischoff, Sophie Möhring, Nicolas Bayer, Dorothée Klein, Adrian Engels, Benjamin Gustav Ziehmer, Julian Stieβ, Pavlo Moroka, Michael Schroda, Marcel Deponte","doi":"10.15698/mic2024.04.821","DOIUrl":"10.15698/mic2024.04.821","url":null,"abstract":"Modular Cloning (MoClo) is based on libraries of standardized genetic parts that can be directionally assembled via Golden Gate cloning in one-pot reactions into transcription units and multigene constructs. Here, a team of bachelor students established a MoClo toolkit for the protist Leishmania tarentolae in the frame of the international Genetically Engineered Machine (iGEM) competition. Our modular toolkit is based on a domesticated version of a commercial LEXSY expression vector and comprises 34 genetic parts encoding various affinity tags, targeting signals as well as fluorescent and luminescent proteins. We demonstrated the utility of our kit by the successful production of 16 different tagged versions of the receptor binding domain (RBD) of the SARS-CoV-2 spike protein in L. tarentolae liquid cultures. While highest yields of secreted recombinant RBD were obtained for GST-tagged fusion proteins 48 h post induction, C-terminal peptide tags were often degraded and resulted in lower yields of secreted RBD. Fusing secreted RBD to a synthetic O-glycosylation SP20 module resulted in an apparent molecular mass shift around 10 kDa. No disadvantage regarding the production of RBD was detected when the three antibiotics of the LEXSY system were omitted during the 48-h induction phase. Furthermore, the successful purification of secreted RBD from the supernatant of L. tarentolae liquid cultures was demonstrated in pilot experiments. In summary, we established a MoClo toolkit and exemplified its application for the production of recombinant proteins in L. tarentolae.","PeriodicalId":18397,"journal":{"name":"Microbial Cell","volume":"11 ","pages":"128-142"},"PeriodicalIF":4.6,"publicationDate":"2024-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11121976/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141155366","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A metagenomic approach to unveil the association between fecal gut microbiota and short-chain fatty acids in diarrhea caused by diarrheagenic Escherichia coli in children. 用元基因组学方法揭示儿童腹泻性大肠埃希氏菌引起的腹泻中粪便肠道微生物群与短链脂肪酸之间的关系。

IF 4.1 3区生物学

Microbial Cell Pub Date : 2024-04-30 eCollection Date: 2024-01-01 DOI: 10.15698/mic2024.04.820

Pablo Gallardo, Mariana Izquierdo, Tomeu Viver, Esteban Bustos-Caparros, Dana Piras, Roberto M Vidal, Hermie M J Harmsen, Mauricio J Farfan

{"title":"A metagenomic approach to unveil the association between fecal gut microbiota and short-chain fatty acids in diarrhea caused by diarrheagenic Escherichia coli in children.","authors":"Pablo Gallardo, Mariana Izquierdo, Tomeu Viver, Esteban Bustos-Caparros, Dana Piras, Roberto M Vidal, Hermie M J Harmsen, Mauricio J Farfan","doi":"10.15698/mic2024.04.820","DOIUrl":"10.15698/mic2024.04.820","url":null,"abstract":"Diarrheagenic Escherichia coli (DEC) is the main cause of diarrhea in children under five years old. The virulence of DEC is tightly regulated by environmental signals influenced by the gut microbiota and its metabolites. Short-chain fatty acids (SCFAs) are the main metabolic product of anaerobic fermentation in the gut, but their role in DEC diarrhea has not yet been established. In this study, we determine the levels of acetate, propionate, and butyrate in stool samples from children with diarrhea caused by DEC, and we identify bacteria from the fecal gut microbiota associated with the production of SCFAs. The microbiota and SCFAs levels in stool samples obtained from 40 children with diarrhea and 43 healthy children were determined by 16S rRNA gene sequencing and HPLC, respectively. Additionally, shotgun metagenomics was used to identify metagenome-assembled genomes (MAGs) in a subgroup of samples. The results showed significantly higher levels of all SCFAs tested in diarrheal samples than in healthy controls. The abundance of Streptococcus sp., Limosilactobacillus, Blautia, Escherichia, Bacteroides, Megamonas, and Roseburia was higher in the DEC group than in healthy individuals. Functional analysis of bacteria and their main metabolic pathways made it possible to identify species MAGs that could be responsible for the detected SCFAs levels in DEC-positive diarrhea. In conclusion, based on our results and published data, we suggest that SCFAs may be important in the crosstalk between the microbiota and DEC pathogens in the gut.","PeriodicalId":18397,"journal":{"name":"Microbial Cell","volume":"11 ","pages":"116-127"},"PeriodicalIF":4.1,"publicationDate":"2024-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11122282/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141155358","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The effect of multiple sclerosis therapy on gut microbiota dysbiosis: a longitudinal prospective study. 多发性硬化症治疗对肠道微生物群失调的影响：一项纵向前瞻性研究。

IF 4.6 3区生物学

Microbial Cell Pub Date : 2024-04-04 eCollection Date: 2024-01-01 DOI: 10.15698/mic2024.03.819

Andreea-Cristina Paraschiv, Vitalie Vacaras, Cristina Nistor, Cristiana Vacaras, Stefan Strilciuc, Dafin F Muresanu

{"title":"The effect of multiple sclerosis therapy on gut microbiota dysbiosis: a longitudinal prospective study.","authors":"Andreea-Cristina Paraschiv, Vitalie Vacaras, Cristina Nistor, Cristiana Vacaras, Stefan Strilciuc, Dafin F Muresanu","doi":"10.15698/mic2024.03.819","DOIUrl":"https://doi.org/10.15698/mic2024.03.819","url":null,"abstract":"Gut microbiota has complex immune functions, related to different pathologies, including multiple sclerosis (MS).This study evaluated the influence of treatments on gut microbiota in people with MS (PwMS). The research comprised 60 participants, including 39 PwMS and 21 healthy controls (HC). Among the PwMS, 20 were prescribed a disease-modifying therapy (DMT), either interferon beta1a or teriflunomide, while 19 received a combination of classical DMT and an immunoglobulin Y (IgY) supplement. For each participant, two sets of gut samples were collected: one at the study's outset and another after two months. Alpha and beta diversity analyses revealed no significant differences between groups. In comparison to the HC, the MS group exhibited an increase in Prevotella stercorea and a decrease in Faecalibacterium prausnitzii. Following treatment, individuals with MS showed enrichment in Lachnospiraceae and Streptococcus. The second sample, compared to the first one, demonstrated an increase in Bifidobacterium angulatum and a decrease in Oscillospira for individuals with MS. Gut microbiota diversity in PwMS is not significantly different to HC.However, specific taxonomic changes indicate the presence of a dysbiosis state. The use of DMTs and immunoglobulin Y supplements may contribute to alterations in microbial composition, potentially leading to the restoration of a healthier microbiome.","PeriodicalId":18397,"journal":{"name":"Microbial Cell","volume":"11 ","pages":"106-115"},"PeriodicalIF":4.6,"publicationDate":"2024-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11026063/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140858506","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0