Microbial Cell最新文献

{"title":"Prohibitins: emerging host targets of bacteria and viruses at the plasma membrane, mitochondria, and cytoplasm.","authors":"Grecia O Rivera-Palomino, Arianne L Theiss","doi":"10.15698/mic2026.06.878","DOIUrl":"10.15698/mic2026.06.878","url":null,"abstract":"<p><p>Ubiquitously expressed Prohibitin-1 (PHB1) and Prohibitin-2 (PHB2) serve pleiotropic functions in cellular processes, including signal transduction, mitochondrial metabolism and dynamics, and lipid raft formation. Located in phospholipid-rich subcellular sites such as the plasma membrane and mitochondrial inner membrane, PHB1 and PHB2 are emerging as important host targets for bacteria and viruses, influencing infection and host responses by these microbes. Here, we present the current understanding of PHB1 and PHB2 in bacterial and viral infection based on the cellular localization of PHBs at the plasma membrane, mitochondria, and cytoplasm. We also discuss the potential of targeting PHBs as therapeutics for bacterial and viral infections.</p>","PeriodicalId":18397,"journal":{"name":"Microbial Cell","volume":"13 ","pages":"218-236"},"PeriodicalIF":4.1,"publicationDate":"2026-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13270817/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148272099","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Outer membrane vesicles in <i>Vibrio</i> species: Roles in biofilm formation and pathogenesis.","authors":"Kumari Shambhavi, Durg Vijai Singh","doi":"10.15698/mic2026.06.877","DOIUrl":"10.15698/mic2026.06.877","url":null,"abstract":"<p><p>Outer membrane vesicles (OMVs) have been increasingly recognized as common mediators of bacterial physiology in Gram-negative bacteria, including <i>Vibrio</i> species. The degree and function of OMV production can differ among strains and even within a single species. The secretion of OMVs is a prevalent trait among many <i>Vibrio</i> species, particularly in pathogenic organisms such as <i>Vibrio cholerae</i>, <i>Vibrio vulnificus</i>, and <i>Vibrio parahaemolyticus</i>. The OMVs released by these organisms are often associated with infection, transport of virulence factors into host cells, defense against stress, biofilm formation, flagella rotation, transportation of active enzymes, signaling molecules in the surrounding environment, and facilitating bacterial translocation. All of these are advantageous to the bacteria. These OMVs also possess immunogenic properties that regulate the innate and adaptive immune responses, which are beneficial to host cells. Few species, such as <i>Vibrio ordalii</i>, <i>Vibrio coralliilyticus</i>, <i>Vibrio natriegens Vibrio alginolyticus, and Vibrio europaeus,</i> have been recently studied for the first time that secrete OMVs; future research is necessary to determine any other activities that these vesicles may possess beyond those that are now documented.</p>","PeriodicalId":18397,"journal":{"name":"Microbial Cell","volume":"13 ","pages":"198-217"},"PeriodicalIF":4.1,"publicationDate":"2026-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13270818/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148272151","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genomic epidemiology of carbapenemase-producing <i>Klebsiella pneumoniae</i> circulating in a Chilean tertiary-care hospital (2021-2022): Molecular characterization, resistance-virulence convergence, and clinical associations.","authors":"Gustavo Araya, Alhejandra Álvarez, Carolina Arellano, Rodrigo Bravo, Abraham Gajardo, Boris Barrera, Francisco Silva, Roberto M Vidal","doi":"10.15698/mic2026.05.876","DOIUrl":"10.15698/mic2026.05.876","url":null,"abstract":"<p><p><i>Klebsiella pneumoniae</i> (Kp) is a Gram-negative bacillus responsible for approximately 10% of nosocomial bacterial infections and one-third of Gram-negative bacterial infections in hospitalized patients. The rise of multidrug-resistant and hypervirulent strains makes it a significant public health issue. This study characterized carbapenem-resistant Kp (CR-Kp) strains isolated at the Hospital Clínico Universidad de Chile (HCUCH) 2021-2022 and explored associations with clinical characteristics. 45 CR-Kp strains from 29 patients in critical care units were analyzed. Mass spectrometry was used for species identification, and antimicrobial susceptibility was assessed by Kirby-Bauer disk diffusion. Clonality was determined using pulsed-field gel electrophoresis (PFGE), and multiplex PCR detected resistance and virulence genes. Clonal strains underwent whole-genome sequencing. PCR revealed the high prevalence of carbapenemase genes and extended-spectrum <math><mi>β</mi></math> -lactamases. PFGE identified nine clones, corresponding to sequence types ST25, ST45, ST307, and ST1161. Frequent virulence factors included siderophores and adhesins, while the capsular serotype K2 was present in 44% of isolates. No classical hypervirulence markers were detected. The presence of <i>blaKPC</i> correlated with more extended hospitalization. These findings reveal convergence between multidrug resistance and adaptive virulence traits rather than classical hypervirulence, highlighting evolving pathogenic strategies in high-risk CR-Kp clones circulating in Chile and emphasizing the need for enhanced molecular surveillance and infection protocols in critical care settings.</p>","PeriodicalId":18397,"journal":{"name":"Microbial Cell","volume":"13 ","pages":"186-197"},"PeriodicalIF":4.1,"publicationDate":"2026-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13270816/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148272103","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The mechanism of Tat-dependent protein translocation.","authors":"Thomas Brüser, Carsten Sanders","doi":"10.15698/mic2026.05.875","DOIUrl":"10.15698/mic2026.05.875","url":null,"abstract":"<p><p>The twin-arginine translocation (Tat) system is the only general pathway for the transport of folded proteins across energized biological membranes. It is found in the bacterial or archaeal cytoplasmic membrane, the plant thylakoid membrane or the inner membrane of plant mitochondria. The biological importance of this translocation system can be exemplified by the fact that all bacterial or plant photosynthesis and photosynthetic oxygen evolution on earth requires this system. Despite many biochemical and biophysical studies, the Tat mechanism has been puzzling since the system was discovered in the 1990ies. Important characteristics of the Tat system could not be explained, and also recent high-resolution structures of the Tat system's core with bound substrate has not led to a general transport mechanism yet. In this integrative review, we attempted to answer the key open questions relevant to the Tat mechanism and thereby developed an in its molecular detail new comprehensive explanation of how folded proteins are translocated across membranes by the Tat system.</p>","PeriodicalId":18397,"journal":{"name":"Microbial Cell","volume":"13 ","pages":"169-185"},"PeriodicalIF":4.1,"publicationDate":"2026-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13186574/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147982107","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sugar-induced cell death (SICD) in <i>Saccharomyces cerevisiae</i>: insights into nitrogen-mediated rescue and apoptotic cell death pathways.","authors":"Raveena Parbhudayal, Hai-Ping Cheng","doi":"10.15698/mic2026.05.874","DOIUrl":"10.15698/mic2026.05.874","url":null,"abstract":"<p><p>Sugar-induced cell death (SICD) is a phenomenon observed in <i>Saccharomyces cerevisiae</i> whereby cells rapidly lose viability in glucose-only solutions. One theory suggests that SICD occurs due to an imbalance in nitrogen and carbon, however, limited studies are available to support this. When stationary phase cells are transferred to glucose-only solutions, cell death resembles that of apoptosis, while exponential phase cells show hallmarks of primary necrosis. Apoptosis in stationary phase cells is independent of the yeast metacaspase, <i>YCA1</i>, however, it remains unknown if SICD occurs through a caspase-independent pathway. Using stationary phase <i>S. cerevisiae</i> BY4741, we showed that SICD can be induced to the same degree with 10 mM or 110 mM glucose. Interestingly, SICD induced by 10 mM of glucose can be protected by supplementation with low concentrations of highly preferred organic nitrogen sources, namely glutamate, glutamine, and arginine, as well as high concentrations of non-preferred organic nitrogen sources. Additionally, cell death can be rescued by deletion of <i>YCA1</i> and genes involved in caspase-independent apoptosis-<i>STE20, NMA111, AIF1,</i> or <i>NUC1</i>. On the other hand, when <i>S. cerevisiae</i> BY4741 is challenged with 110 mM glucose, SICD can only be rescued by supplementation with the same preferred organic nitrogen sources or deletion of <i>AIF1</i> or <i>NMA111.</i> In all cases, protection is associated with a decrease in intracellular ROS and preservation of membrane integrity. Taken together, 110 mM glucose results in a catastrophic cell death phenotype that is more difficult to rescue, and nuclear localization of Aif1p and Nma111p is important for cell death in response to glucose.</p>","PeriodicalId":18397,"journal":{"name":"Microbial Cell","volume":"13 ","pages":"155-168"},"PeriodicalIF":4.1,"publicationDate":"2026-05-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13186538/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147982152","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"From the gut to the lungs: The role of gut microbiota in chronic obstructive pulmonary disease and related research progress.","authors":"Simin Yang, Shuting Zeng, Yongan Deng, Xiaodong Duan, Chengkai Chen, Luyun Sun, Yongkang Qiao, Zunpeng Shu","doi":"10.15698/mic2026.04.873","DOIUrl":"10.15698/mic2026.04.873","url":null,"abstract":"<p><p>Chronic Obstructive Pulmonary Disease (COPD) is a progressive respiratory disease with high morbidity and mortality. Existing treatment methods are difficult to effectively curb disease progression, highlighting the urgency to explore new pathogenesis mechanisms and therapeutic targets. With the development of microbiomics, the proposal of the \"gut-lung axis\" concept has provided a brand-new perspective for understanding the pathological mechanisms of COPD, revealing that the gut and lungs maintain a close connection through pathways such as immune regulation and metabolic interaction. This article systematically elaborates on the association between gut microbiota and COPD: First, it deeply analyzes the pathological interaction between the gut and lungs from the perspective of the gut-lung axis. On this basis, it examines the characteristic changes in gut microbiota and their metabolites in COPD patients, explores the key influencing factors driving such microbiota dysbiosis, and further systematically explains the core mechanisms by which gut microbiota contribute to the occurrence and progression of COPD. Finally, it focuses on strategies for the prevention and treatment of COPD based on gut microbiota regulation, and prospects their clinical application potential. The purpose of this article is to provide new ideas and directions for the basic research and clinical practice of COPD by comprehensively sorting out the association between gut microbiota and COPD, thereby helping to improve the current status of COPD prevention and treatment.</p>","PeriodicalId":18397,"journal":{"name":"Microbial Cell","volume":"13 ","pages":"138-154"},"PeriodicalIF":4.1,"publicationDate":"2026-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13093826/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147775120","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"TOR-dependent regulation of the yeast homolog of the juvenile Batten Disease-associated gene <i>CLN3</i>.","authors":"Vijaykumar Pillalamarri, Samuel W M Gatesy, Amanda E Grassel, Lucienna Wolf, Justin P Whalley, David M Mueller","doi":"10.15698/mic2026.03.872","DOIUrl":"10.15698/mic2026.03.872","url":null,"abstract":"<p><p>The Juvenile form of Batten disease is a neurodegenerative disease with symptoms starting in the first decade and ending in death in the third decade of life. The gene defective in this form of Batten disease, <i>CLN3</i>, is conserved in eukaryotes, suggesting that the gene product serves a basic function in the cell, though the function is unknown. We have investigated the expression and regulation of the yeast homolog BTN1. Reanalysis of publicly available gene expression data suggests that transcription of BTN1 increases in response to oxidative stress, treatment with rapamycin or arsenate, amino acid starvation, and sporulation conditions. Similar to GCN4, there are upstream open reading frames (uORF) in front of BTN1, suggesting translational regulation. We developed reporter strains in which the HIS3 open reading frame replaced that of the BTN1 gene, with and without the uORFs. These reporters show that one or more of the uORFs decrease the expression of the HIS3 reporter. When expressed in the reporter strain using a high copy vector, GCN3, tRNA <math><msup><mrow></mrow> <mrow><mi>Arg</mi></mrow> </msup> </math> , and tRNA <math><msup><mrow></mrow> <mrow><mi>Leu</mi></mrow> </msup> </math> , increase expression, suggesting the involvement of the TORC1 pathway. BIT61 abuts BTN1 but is encoded on the opposite strand; 3' RACE analysis indicates that the mRNA of BIT61 overlaps with that of BTN1. BIT61 is involved in the TORC2 pathway, which interacts with the TORC1 pathway, suggesting a possible cis-acting mechanism of co-regulation. Lastly, we demonstrate that a yeast strain with a null mutation in BTN1 is sensitive to selective amino acid starvation, further supporting the association of BTN1 with TORC1.</p>","PeriodicalId":18397,"journal":{"name":"Microbial Cell","volume":"13 ","pages":"131-147"},"PeriodicalIF":4.1,"publicationDate":"2026-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13093828/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147775092","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metagenomic and microbiological analyses of historical manuscripts for bacterial community profiling and bacteria-related biodeterioration assessment.","authors":"Esra Keles, Ozge Celik","doi":"10.15698/mic2026.03.871","DOIUrl":"10.15698/mic2026.03.871","url":null,"abstract":"<p><p>Bacteria are important agents in the biodeterioration of cultural heritage objects, including historical manuscripts. Characterizing bacterial communities and generating robust microbiological data has therefore become crucial for conservation and restoration strategies. In this study, we investigated the bacterial communities associated with biodeterioration in six historical manuscripts using both culture-dependent and culture-independent (Illumina MiSeq) approaches. Culture-dependent methods yielded only 16 viable and culturable isolates, highlighting the limitations of traditional techniques. In contrast, metagenomic analysis revealed a far richer and more diverse bacterial community, capturing both living and non-living microbial traces accumulated over centuries. Bacterial genera with known cellulolytic and/or proteolytic activities, such as <i>Bacillus, Stenotrophomonas, Pseudomonas</i> and <i>Acinetobacter</i>, were identified as part of a core microbiome commonly associated with paper deterioration. High abundances of gut-associated bacteria (<i>Prevotella, Faecalibacterium, Bacteroides, Porphyromonas</i>) and human-related taxa (<i>Staphylococcus, Streptococcus, Cutibacterium</i>) indicated extensive historical human handling. A notable finding was the detection of <i>Pseudonocardia broussonetiae</i>, an endophytic bacterium associated with paper mulberry (<i>Broussonetia papyrifera</i>), suggesting the possible use of this plant as a papermaking material in one manuscript. This represents an important contribution to understanding Islamic paper production. Overall, our results demonstrate that effective conservation strategies require a detailed understanding of each manuscript's microbial ecology, together with evidence of past environmental conditions, handling history, and production materials.</p>","PeriodicalId":18397,"journal":{"name":"Microbial Cell","volume":"13 ","pages":"117-130"},"PeriodicalIF":4.1,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12958588/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147365825","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Overcoming phagocytosis resistance of hypervirulent <i>Klebsiella pneumoniae</i> by directly targeting capsules.","authors":"Shogo Tsubaki, Touya Toyomoto, Rika Tanaka, Jin Imai, Juntaro Matsuzaki, Katsuto Hozumi, Hitoshi Tsugawa","doi":"10.15698/mic2026.02.870","DOIUrl":"10.15698/mic2026.02.870","url":null,"abstract":"<p><p>Capsular polysaccharides (CPS) are key virulence factors in <i>Klebsiella pneumoniae</i> and are closely associated with the K1 and K2 hypervirulent serotypes. Herein, we demonstrate that introducing nonspecific RNA (sgRNA) into <i>K. pneumoniae</i> ATCC43816 (Kp-pET-sgRNA), a K2 serotype strain classified as hypervirulent (hvKp), results in marked capsule loss and reduced hypermucoviscosity. Capsule loss and reduced hypermucoviscosity in Kp-pET-sgRNA were confirmed by comparison with the wild-type strain (Kp-WT) using transmission electron microscopy, hypermucoviscosity assays, and string tests. Mechanistically, we found that overexpression of sgRNA by introducing the pET-sgRNA plasmid led to gene deletion in the <i>rmpADC</i> operon, a key virulence determinant located on mobile chromosomal elements. Additionally, the mRNA expression of <i>manC</i>, which are chromosomal <i>cps</i>-related genes, was significantly repressed. In contrast, introduction of pET-sgRNA did not alter the mRNA expression of <i>galF</i> or <i>wzi</i>. The results revealed that capsule loss and reduced hypermucoviscosity in Kp-pET-sgRNA resulted from synergistic downregulation of both the <i>rmpADC</i> operon and <i>manC</i>. Loss of capsule synthesis and reduction of hypermucoviscosity in <i>K. pneumoniae</i> caused by sgRNA overexpression significantly decreased resistance to phagocytosis by macrophages but did not influence susceptibility to meropenem or colistin. The findings reveal an unexpected consequence of plasmid-mediated sgRNA introduction, where overexpression of sgRNA abolishes phagocytic resistance by disrupting capsule biosynthesis and reducing hypermucoviscosity in <i>K. pneumoniae</i>. This study highlights a promising strategy for disarming hypervirulent <i>K. pneumoniae</i> by directly targeting its key virulence factors and provides novel insights into antibacterial therapeutic approaches against this clinically significant pathogen.</p>","PeriodicalId":18397,"journal":{"name":"Microbial Cell","volume":"13 ","pages":"103-116"},"PeriodicalIF":4.1,"publicationDate":"2026-02-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12925632/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147276485","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protein arginine methyltransferases in protozoan parasites: a new path for antiparasitic chemotherapy?","authors":"Gustavo D Campagnaro, Sébastien Pomel","doi":"10.15698/mic2026.02.869","DOIUrl":"10.15698/mic2026.02.869","url":null,"abstract":"<p><p>Protein arginine methyltransferases (PRMTs) catalyse the transference of methyl groups from S-adenosylmethionine to arginine residues in substrate proteins, a post-translational modification widespread among eukaryotes. The change in size and hydrophobicity of the methylated arginine residue impacts on how a protein interacts with other macromolecules and affects several cellular processes, including intracellular signaling, DNA replication and repair, and control of gene expression. As a result, PRMTs became attractive targets for chemotherapy, and several PRMT inhibitors are going through clinical trials for cancer treatment. In protozoan parasites, PRMTs play fundamental roles during development, stage differentiation and infection processes. We here review the activity and the relevance of PRMTs for the survival of pathogenic kinetoplastids, apicomplexans and amoebas, highlight differences observed between PRMTs expressed in these organisms and their mammalian orthologues, and suggest that these enzymes can be exploited to combat parasitic infections. We propose that the arsenal of inhibitors developed to target mammalian PRMTs could be reassigned to allow the identification of new scaffolds to be explored as antiparasitic agents, either as sole chemotherapy or by improving the effectiveness of current antiparasitic drugs.</p>","PeriodicalId":18397,"journal":{"name":"Microbial Cell","volume":"13 ","pages":"86-102"},"PeriodicalIF":4.1,"publicationDate":"2026-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12925635/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147276453","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}