Microbial Cell最新文献

An adenine model of inborn metabolism errors alters TDP-43 aggregation and reduces its toxicity in yeast revealing insights into protein misfolding diseases. 先天性代谢错误的腺嘌呤模型改变了TDP-43聚集并降低了其在酵母中的毒性，揭示了蛋白质错误折叠疾病的见解。

IF 4.1 3区生物学

Microbial Cell Pub Date : 2025-05-22 eCollection Date: 2025-01-01 DOI: 10.15698/mic2025.05.850

Sangeun Park, Sei-Kyoung Park, Peter Blair, Susan W Liebman

{"title":"An adenine model of inborn metabolism errors alters TDP-43 aggregation and reduces its toxicity in yeast revealing insights into protein misfolding diseases.","authors":"Sangeun Park, Sei-Kyoung Park, Peter Blair, Susan W Liebman","doi":"10.15698/mic2025.05.850","DOIUrl":"10.15698/mic2025.05.850","url":null,"abstract":"TDP-43 is linked to human diseases such as amyotrophic lateral sclerosis (ALS) and frontotemporal degeneration (FTD). Expression of TDP-43 in yeast is known to be toxic, cause cells to elongate, form liquid-like aggregates, and inhibit autophagy and TOROID formation. Here, we used the apt1∆ aah1∆ yeast model of inborn errors of metabolism, previously shown to lead to intracellular adenine accumulation and adenine amyloid-like fiber formation, to explore interactions with TDP-43. Results show that the double deletion shifts the TDP-43 aggregates from liquid-like droplets toward a more amyloid-like state. At the same time the deletions reduce TDP-43's effects on toxicity, cell morphology, autophagy, and TOROID formation without affecting the level of TDP-43. This suggests that the liquid-like droplets rather than amyloid-like TDP-43 aggregates are responsible for the deleterious effects in yeast. How the apt1∆ aah1∆ deletions alter TDP-43 aggregate formation is not clear. Possibly, it results from adenine and TDP-43 fiber interactions as seen for other heterologous fibers. This work offers new insights into the potential interactions between metabolite-based amyloids and pathological protein aggregates, with broad implications for understanding protein misfolding diseases.","PeriodicalId":18397,"journal":{"name":"Microbial Cell","volume":"12 ","pages":"119-130"},"PeriodicalIF":4.1,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12105045/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144150937","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advancements in vaginal microbiota, Trichomonas vaginalis, and vaginal cell interactions: Insights from co-culture assays. 阴道微生物群、阴道毛滴虫和阴道细胞相互作用的进展：来自共培养试验的见解。

IF 4.1 3区生物学

Microbial Cell Pub Date : 2025-05-15 eCollection Date: 2025-01-01 DOI: 10.15698/mic2025.05.849

Fernanda Gomes Cardoso, Tiana Tasca

{"title":"Advancements in vaginal microbiota, Trichomonas vaginalis, and vaginal cell interactions: Insights from co-culture assays.","authors":"Fernanda Gomes Cardoso, Tiana Tasca","doi":"10.15698/mic2025.05.849","DOIUrl":"10.15698/mic2025.05.849","url":null,"abstract":"Vaginal microbiota involves seven communities-state types (CST), four dominated by Lactobacillus. L. crispatus, particularly, offers enhanced protection against infections. Recurrent vulvovaginal candidiasis and trichomoniasis affect millions of people annually, often asymptomatically, facilitating infection spread and leading complications. Co-culture, the technique of cultivating different microbial populations together to mimic real-life conditions, enables the study of microorganism interactions, including inhibitory or promotive effects on pathogens. This review compiles data on co-culture techniques to analyze interactions among Lactobacillus spp., Candida spp., and Trichomonas vaginalis. PubMed was searched using medical subject headings (MESH) terms, 'co-culture', 'coculture,' 'cocultivation,' 'co-incubation,' and 'Trichomonas vaginalis', 'Candida spp.', 'Lactobacillus spp.'. Articles were selected based on relevance to vaginal health, English language, availability, and use of co-culture or co-incubation techniques in the past 24 years. Co-culture and co-incubation studies over the past 24 years have advanced our understanding of microbiota-host, pathogen-host, and pathogen-host-microbiota interactions. These studies reveal that microbiota composition impacts infections, with the microbiota producing substances against pathogens and pathogens developing stress tolerance mechanisms. They elucidate pathogen virulence factors, interactions with immune cells, and how ecological relationships between microorganisms can enhance pathogenicity.","PeriodicalId":18397,"journal":{"name":"Microbial Cell","volume":"12 ","pages":"109-118"},"PeriodicalIF":4.1,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12094111/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144120064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Influence of cervicovaginal microbiota on Chlamydia trachomatis infection dynamics. 宫颈阴道微生物群对沙眼衣原体感染动态的影响。

IF 4.1 3区生物学

Microbial Cell Pub Date : 2025-04-15 eCollection Date: 2025-01-01 DOI: 10.15698/mic2025.04.848

Emily Hand, Indriati Hood-Pishchany, Toni Darville, Catherine M O'Connell

{"title":"Influence of cervicovaginal microbiota on Chlamydia trachomatis infection dynamics.","authors":"Emily Hand, Indriati Hood-Pishchany, Toni Darville, Catherine M O'Connell","doi":"10.15698/mic2025.04.848","DOIUrl":"https://doi.org/10.15698/mic2025.04.848","url":null,"abstract":"The cervicovaginal microbiome (CVM) is increasingly being considered as an important aspect of women's health, particularly in relation to the risk and progression of sexually transmitted infections (STIs). CVM composition varies significantly between individuals and is shaped by factors including diet, age, environmental exposures, and lifestyle. Understanding these influences may shed light on how microbial imbalances contribute to infection susceptibility and the development of reproductive health disorders. Five distinct community state types (CSTs) classify common CVM compositions. Most CSTs (I, II, III, V) are characterized by a dominant Lactobacillus species and are associated with better or neutral reproductive health, including reduced coincident detection of STIs such as Chlamydia trachomatis. In contrast, CST IV is composed of diverse, predominantly anaerobic, microbial species and is associated with CVM dysbiosis, bacterial vaginosis, and a heightened risk of STI acquisition. This review examines the complex interplay between the CVM, C. trachomatis infection, and host immune responses, highlighting the role of metabolites such as short-chain and long-chain fatty acids, indole, and iron in modulating pathogen survival and host defenses. Additionally, the impacts of CVM composition on C. trachomatis persistence, ascension, and clearance are discussed, alongside co-infection dynamics with pathogens like Neisseria gonorrhoeae and Mycoplasma genitalium.","PeriodicalId":18397,"journal":{"name":"Microbial Cell","volume":"12 ","pages":"93-108"},"PeriodicalIF":4.1,"publicationDate":"2025-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12042374/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144016402","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microbiota and metabolome dynamics induced by Shiga toxin-producing E. coli in an in vitro model of an infant's colon. 产志贺毒素大肠杆菌在婴儿结肠体外模型中诱导的微生物群和代谢动力学。

IF 4.1 3区生物学

Microbial Cell Pub Date : 2025-04-14 eCollection Date: 2025-01-01 DOI: 10.15698/mic2025.04.847

Mariana Izquierdo, Deborah O'Sullivan, Ophélie Uriot, Morgane Brun, Claude Durif, Sylvain Denis, Pablo Gallardo, Cormac G M Gahan, Lucie Etienne-Mesmin, Stéphanie Blanquet-Diot, Mauricio J Farfan

{"title":"Microbiota and metabolome dynamics induced by Shiga toxin-producing E. coli in an in vitro model of an infant's colon.","authors":"Mariana Izquierdo, Deborah O'Sullivan, Ophélie Uriot, Morgane Brun, Claude Durif, Sylvain Denis, Pablo Gallardo, Cormac G M Gahan, Lucie Etienne-Mesmin, Stéphanie Blanquet-Diot, Mauricio J Farfan","doi":"10.15698/mic2025.04.847","DOIUrl":"https://doi.org/10.15698/mic2025.04.847","url":null,"abstract":"Shiga toxin-producing Escherichia coli (STEC) is a major food-borne pathogen causing human diseases ranging from diarrhea to life-threatening complications, mainly in young children. Colonization, virulence, and interactions of STEC strains with human gut microbiota are pivotal during infection but remain poorly described, particularly in children, the most affected population. In this work, we evaluated changes in the microbiota and metabolome composition in the in vitro gut model: Toddler ARtificial COLon (T-ARCOL) infected with EHEC O157:H7 strain EDL 933. Stool samples collected from children with STEC-positive diarrhea and stool from the same children after recovery from the diarrheal episode (n=5) were used to inoculate the T-ARCOL model. STEC colonization was progressively reduced throughout fermentation in T-ARCOL with diarrhea or recovery fecal samples. Beta diversity showed that the diarrhea-associated microbiota was significantly distinct from the recovery microbiota and exhibited a lower α-diversity. In contrast to recovery conditions, diarrheal conditions were characterized by an increased abundance of potential pathobionts such as members of the Clostridiaceae family and higher acetate, succinate, and N-acetylneuraminic acid levels. Our results provide new evidence of the impact of EHEC in the microbiota and metabolome dynamics in an in vitro gut model that could be useful in understanding their physiopathology in this at-risk population, considering inter-individual variabilities in gut microbiota.","PeriodicalId":18397,"journal":{"name":"Microbial Cell","volume":"12 ","pages":"76-92"},"PeriodicalIF":4.1,"publicationDate":"2025-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12042126/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144025164","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unveiling the molecular architecture of the mitochondrial respiratory chain of Acanthamoeba castellanii. 揭示棘阿米巴线粒体呼吸链的分子结构。

IF 4.1 3区生物学

Microbial Cell Pub Date : 2025-03-31 eCollection Date: 2025-01-01 DOI: 10.15698/mic2025.03.846

Christian Q Scheckhuber, Sutherland K Maciver, Alvaro de Obeso Fernandez Del Valle

{"title":"Unveiling the molecular architecture of the mitochondrial respiratory chain of Acanthamoeba castellanii.","authors":"Christian Q Scheckhuber, Sutherland K Maciver, Alvaro de Obeso Fernandez Del Valle","doi":"10.15698/mic2025.03.846","DOIUrl":"https://doi.org/10.15698/mic2025.03.846","url":null,"abstract":"Acanthamoeba castellanii is a ubiquitous free-living amoeba that can cause severe infections in humans. Unlike most other organisms, A. castellanii possesses a \"complete\" mitochondrial respiratory chain, meaning it con-tains several additional enzymes that contribute to its metabolic versa-tility and survival in diverse environments. This review provides a com-prehensive overview of the mitochondrial respiratory chain in A. castellanii, focusing on the key alternative components in-volved in oxidative phosphorylation and their roles in energy metabo-lism, stress response, and adaptation to various conditions. The func-tional characterization of the alternative oxidase (AOX), uncoupling pro-tein (UCP), and alternative NAD(P)H dehydrogenases, highlight their roles in reducing oxidative stress, modulating proton gradients, and adapting to changes in temperature and nutrient availability. These pro-teins and systems serve a role in the survival of A. castel-lanii under stressful conditions such as starvation and cold con-ditions. Further knowledge of the respiratory chain of the amoeba has potential implications for understanding the evolution of mitochondrial respiration and developing new therapies for treating Acanthamoeba infections.","PeriodicalId":18397,"journal":{"name":"Microbial Cell","volume":"12 ","pages":"65-75"},"PeriodicalIF":4.1,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12040293/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143970475","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ampicillin treatment in persister cell studies may cause non-physiological artifacts. 在持久性细胞研究中氨苄西林治疗可能引起非生理性伪影。

IF 4.1 3区生物学

Microbial Cell Pub Date : 2025-03-20 eCollection Date: 2025-01-01 DOI: 10.15698/mic2025.03.845

Michel Fasnacht, Hena Comic, Isabella Moll

{"title":"Ampicillin treatment in persister cell studies may cause non-physiological artifacts.","authors":"Michel Fasnacht, Hena Comic, Isabella Moll","doi":"10.15698/mic2025.03.845","DOIUrl":"https://doi.org/10.15698/mic2025.03.845","url":null,"abstract":"Persister cells are a clinically relevant sub-population of an isogenic bacterial culture that is tolerant to bactericidal antibiotics. With the aim to investigate the ribosomal protein content of persister cells, we employed the bacteriolytic properties of ampicillin to separate persister from sensitive cells. Thereby, we observed processing of several ribosomal proteins. Promisingly, we detected a variant of the large subunit protein uL2 that lacks the last 59 amino acids from its C-terminus (tL2) and which previously has been described as an inhibitor of DNA replication in vitro. Considering the increasing number of moonlighting functions described for ribosomal proteins, we investigated a potential regulatory role of tL2 in persister cells after ampicillin treatment. In contrast to our assumption, our findings show that the generation of tL2 after ampicillin treatment must be attributed to proteolysis upon cell lysis. Ultimately, no tL2 was detected intracellularly of purified persister cells isolated by an improved protocol employing proteinase K treatment. We therefore exclude the possibility of tL2 regulating DNA replication in ampicillin tolerant E. coli cells. Nevertheless, this study clearly highlights the necessity of further purification steps in addition to ampicillin treatment for the study of persister cells and invites for the careful re-examination of previously published results.","PeriodicalId":18397,"journal":{"name":"Microbial Cell","volume":"12 ","pages":"53-64"},"PeriodicalIF":4.1,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12039935/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144017846","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Clostridium scindens promotes gallstone formation by inducing intrahepatic neutrophil extracellular traps through CXCL1 produced by colonic epithelial cells. scindens梭状芽胞杆菌通过结肠上皮细胞产生的CXCL1诱导肝内中性粒细胞胞外陷阱，促进胆结石的形成。

IF 4.1 3区生物学

Microbial Cell Pub Date : 2025-03-19 eCollection Date: 2025-01-01 DOI: 10.15698/mic2025.03.844

Wenchao Yao, Yuanhang He, Zhihong Xie, Qiang Wang, Yang Chen, Jingjing Yu, Xuxu Liu, Dongbo Xue Xue, Wang Liyi, Chenjun Hao

{"title":"Clostridium scindens promotes gallstone formation by inducing intrahepatic neutrophil extracellular traps through CXCL1 produced by colonic epithelial cells.","authors":"Wenchao Yao, Yuanhang He, Zhihong Xie, Qiang Wang, Yang Chen, Jingjing Yu, Xuxu Liu, Dongbo Xue Xue, Wang Liyi, Chenjun Hao","doi":"10.15698/mic2025.03.844","DOIUrl":"https://doi.org/10.15698/mic2025.03.844","url":null,"abstract":"Cholelithiasis is one of the most common diseases of the biliary system. Neutrophil extracellular traps (NETs) in the liver play an important role in accelerating the formation of gallstones. The upstream mechanism of NETs formation remains unclear. In this study, 16S rRNA sequencing was used to screen the differential gut microbiota in mice with gallstones. Transcriptome sequencing was used to screen the differentially expressed core genes and signalling pathways of Clostridium scindens that acted on human colonic epithelial cells. Western blotting was used to verify the protein expression of TLR2 and the NF-κB pathway. RT-PCR was used to verify the mRNA expression of TLR2, CXCL1 and the NF-κB pathway. ELISA was used to verify CXCL1 expression in the supernatant or portal vein blood of mice. Immunofluorescence was used to detect NETs formation in cocultured neutrophils in vitro or in mouse livers. Clostridium scindens was the key differential strain in the formation of gallstones in mice. After treatment with Clostridium scindens, both in vitro and in vivo, the expression of TLR2 was upregulated, the secretion of CXCL1 was increased by regulating the NF-κB pathway. Finally, the formation of NETs and stones was significantly increased. This study reveals a new mechanism of the gut-liver immune axis in the formation of gallstones. Clostridium scindens acts on colonic epithelial cells through TLR2 to regulate the NF-κB pathway and increase the secretion of CXCL1. CXCL1 enters the liver via the portal vein and increases the formation of NETs in the liver, thereby accelerating gallstone formation.","PeriodicalId":18397,"journal":{"name":"Microbial Cell","volume":"12 ","pages":"37-52"},"PeriodicalIF":4.1,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12041793/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144003374","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

It takes four to tango: the cooperative adventure of scientific publishing. 探戈需要四个人：科学出版的合作冒险。

IF 4.1 3区生物学

Microbial Cell Pub Date : 2025-02-21 eCollection Date: 2025-01-01 DOI: 10.15698/mic2025.02.843

Didac Carmona-Gutierrez, Katharina Kainz, Frank Madeo

引用次数: 0

Paving the way for new antimicrobial peptides through molecular de-extinction. 通过分子去灭绝为新的抗菌肽铺平道路。

IF 4.1 3区生物学

Microbial Cell Pub Date : 2025-02-20 eCollection Date: 2025-01-01 DOI: 10.15698/mic2025.02.841

Karen O Osiro, Abel Gil-Ley, Fabiano C Fernandes, Kamila B S de Oliveira, Cesar de la Fuente-Nunez, Octavio L Franco

引用次数: 0

Integrative Omics reveals changes in the cellular landscape of peroxisome-deficient pex3 yeast cells. 整合组学揭示了过氧化物酶体缺陷酵母细胞的细胞景观变化。

IF 4.1 3区生物学

Microbial Cell Pub Date : 2025-02-20 eCollection Date: 2025-01-01 DOI: 10.15698/mic2025.02.842

Tjasa Kosir, Hirak Das, Marc Pilegaard Pedersen, Ann-Kathrin Richard, Marco Anteghini, Vitor Martins Dos Santos, Silke Oeljeklaus, Ida J van der Klei, Bettina Warscheid

{"title":"Integrative Omics reveals changes in the cellular landscape of peroxisome-deficient pex3 yeast cells.","authors":"Tjasa Kosir, Hirak Das, Marc Pilegaard Pedersen, Ann-Kathrin Richard, Marco Anteghini, Vitor Martins Dos Santos, Silke Oeljeklaus, Ida J van der Klei, Bettina Warscheid","doi":"10.15698/mic2025.02.842","DOIUrl":"10.15698/mic2025.02.842","url":null,"abstract":"Peroxisomes are organelles that are crucial for cellular metabolism, but they also play important roles in non-metabolic processes such as signalling, stress response or antiviral defense. To uncover the consequences of peroxisome deficiency, we compared Saccharomyces cerevisiae wild-type with pex3 cells, which lack peroxisomes, employing quantitative proteomics and transcriptomics technologies. Cells were grown on acetate, a carbon source that requires peroxisomal enzymes of the glyoxylate cycle to generate energy and essential carbohydrates, and that does not repress the expression of peroxisomal genes. Our integrative omics analysis reveals that the absence of peroxisomes induces distinct responses at the level of the transcriptome and proteome. Transcripts of genes and corresponding proteins that are associated with peroxisomal β-oxidation were mostly increased in pex3 cells. In contrast, levels of peroxins were regulated at protein but not at transcript level. Membrane-bound peroxins were reduced, whereas the soluble receptors Pex5 and Pex7 were increased in abundance in pex3 cells. Interestingly, we found several non-peroxisomal transcript and proteins regulated in pex3 cells including mitochondrial proteins involved in respiration or import processes, which led to the identification of the mitochondrial pyruvate carrier Mpc1/3 as so far unnoticed transporter present in the peroxisomal membrane. Our results reveal the impact of the absence of peroxisomes in pex3 yeast cells and represent a rich resource of genes/proteins for follow-up studies to obtain a deeper understanding of peroxisome biology in a cellular context.","PeriodicalId":18397,"journal":{"name":"Microbial Cell","volume":"12 ","pages":"9-33"},"PeriodicalIF":4.1,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11862644/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143516106","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0