Microbial Cell最新文献_第3页

VapA/Scs2 sustains polarized growth in Aspergillus nidulans by maintaining AP-2-mediated apical endocytosis. VapA/Scs2通过维持ap -2介导的根尖内吞作用来维持中性曲霉的极化生长。

IF 4.1 3区生物学

Microbial Cell Pub Date : 2026-02-04 eCollection Date: 2026-01-01 DOI: 10.15698/mic2026.02.868

Xenia Georgiou, Sofia Politi, Sotiris Amillis, George Diallinas

{"title":"VapA/Scs2 sustains polarized growth in Aspergillus nidulans by maintaining AP-2-mediated apical endocytosis.","authors":"Xenia Georgiou, Sofia Politi, Sotiris Amillis, George Diallinas","doi":"10.15698/mic2026.02.868","DOIUrl":"10.15698/mic2026.02.868","url":null,"abstract":"Growth of filamentous fungi is highly polarized requiring the coordinated apical delivery of cell wall components and plasma membrane (PM) material, primarily lipids and proteins, to hyphal tips via conventional vesicular secretion. Fungal growth also requires the tight coordination of exocytosis (secretion) with endocytosis and recycling of proteins and lipids, which occurs in a defined region behind the growing tip known as the endocytic collar. Here, we genetically characterized proteins tentatively implicated in the formation of endoplasmic reticulum-plasma membrane (ER-PM) contact sites, including Scs2/VAP, tricalbins and Ist2 homologues, in Aspergillus nidulans. We showed that among these proteins, only the single Scs2/VapA homologue is essential for normal fungal growth, and this requirement is due to the critical role of VapA in maintaining the polarized localization of apical cargoes, such as the lipid flippases DnfA and DnfB or the SNARE protein SynA. In <math><mi>Δ</mi></math> vapA mutants, these cargoes lose their polarized localization, a phenotype that correlates with the mislocalization of the AP-2 cargo adaptor complex, which is essential for the endocytosis and recycling of apical membrane components. Further analysis provides evidence linking the defect in apical cargo endocytosis observed in <math><mi>Δ</mi></math> vapA mutants to altered membrane lipid partitioning, suggesting that VapA contributes to lipid domain organization critical for cargo recycling. Strikingly, deletion of VapA does not impair the localization or endocytosis of non-polarized (subapical) plasma membrane transporters, indicating that the trafficking and biogenesis of polarized (apical) versus non-polarized (subapical) cargoes are differentially dependent on membrane lipid composition and domain-specific organization.","PeriodicalId":18397,"journal":{"name":"Microbial Cell","volume":"13 ","pages":"63-85"},"PeriodicalIF":4.1,"publicationDate":"2026-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12925633/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147276448","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genetic make-up and regulation of the L-lysine biosynthesis pathway in Vibrio natriegens. 营养弧菌l -赖氨酸生物合成途径的基因组成和调控。

IF 4.1 3区生物学

Microbial Cell Pub Date : 2026-02-03 eCollection Date: 2026-01-01 DOI: 10.15698/mic2026.02.867

Elly Straube, Johannes Radde, Thi Van Anh Tran, Negin Keihani Yazdi, Rubén Crespo Blanco, Ha Thanh Le, Cláudio J R Frazão, Thomas Walther

{"title":"Genetic make-up and regulation of the L-lysine biosynthesis pathway in Vibrio natriegens.","authors":"Elly Straube, Johannes Radde, Thi Van Anh Tran, Negin Keihani Yazdi, Rubén Crespo Blanco, Ha Thanh Le, Cláudio J R Frazão, Thomas Walther","doi":"10.15698/mic2026.02.867","DOIUrl":"10.15698/mic2026.02.867","url":null,"abstract":"Vibrio natriegens, the fastest growing non-pathogenic microorganism known to date, has emerged as a highly promising chassis strain for synthetic biology and biotechnology applications. This study analysed the make-up and regulation of the biosynthetic pathway for L-lysine and related L-aspartate family amino acids (AFAAs) in V. natriegens DSM759 to provide a comprehensive basis for future metabolic engineering endeavours aiming at developing this strain into an amino acid overproducer. The compilation of automatically annotated genome sequencing data revealed the presence of gene duplicates encoding putative isozymes for multiple enzymatic reactions within these pathways. The physiological role of these isozymes was analysed via growth phenotyping of corresponding gene deletion mutants as well as enzymatic assays. We verified the presence of a previously unknown mono-functional aspartate kinase isozyme, here termed Vn.LysC2, which was shown to be insensitive to allosteric inhibition by any AFAA. In addition, functional duplicates of L-aspartate semialdehyde dehydrogenase and dihydrodipicolinate synthase enzymes were identified. RNA sequencing experiments were used to elucidate the transcriptional regulation mediated by AFAAs on both their corresponding biosynthetic pathways as well as on the global metabolism. The presence of L-lysine, L-threonine, L-isoleucine and L-methionine resulted in the transcriptional repression of their respective biosynthetic pathways. A global analysis of the transcriptional response revealed that the transcriptional response to L-lysine and L-isoleucine was characterised by a high degree of specificity (four and seven differentially expressed genes, respectively), while L-methionine and L-threonine supplementation affected the expression of a larger number of genes (37 and 60 differentially expressed genes).","PeriodicalId":18397,"journal":{"name":"Microbial Cell","volume":"13 ","pages":"44-62"},"PeriodicalIF":4.1,"publicationDate":"2026-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12925634/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147276507","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Regulation of extracellular vesicles for protein secretion in Aspergillus nidulans. 细粒曲霉细胞外囊泡对蛋白质分泌的调控。

IF 3.9 3区生物学

Microbial Cell Pub Date : 2026-01-28 eCollection Date: 2026-01-01 DOI: 10.15698/mic2026.01.866

Rebekkah E Pope, Patrick Ballmann, Lisa Whitworth, Rolf A Prade

{"title":"Regulation of extracellular vesicles for protein secretion in Aspergillus nidulans.","authors":"Rebekkah E Pope, Patrick Ballmann, Lisa Whitworth, Rolf A Prade","doi":"10.15698/mic2026.01.866","DOIUrl":"10.15698/mic2026.01.866","url":null,"abstract":"Fungi were among the first eukaryotes to transition from aquatic to terrestrial life, developing multicellular hyphae, polar growth, and expanded secretomes for nutrient processing, defense, and symbiosis. We present a reliable method for purifying and characterizing extracellular vesicles (EVs) from Aspergillus nidulans and demonstrate that the induction of xylanase C is associated with increased EV release and EV-associated enzymatic activity. Using a mCherry reporter replacing xylanase C, we generalized this effect, showing that reporter induction increases EV production and reporter loading into EVs. This phenomenon primarily depends on the signal peptide (SP), suggesting that the induction of endoplasmic reticulum (ER)- trafficked proteins has a pronounced effect on EV production and cargo loading. We speculate that EV biogenesis may originate at the ER, where ER-translated proteins could be selectively loaded into vesicles and subsequently trafficked directly to the plasma membrane or through multivesicular bodies (MVBs). EV secretion is minimal in the first 24-48 hours but increases later in growth, coinciding with biofilm formation. This timing allows A. nidulans to modify the secretome, adapting it to new nutrient sources.","PeriodicalId":18397,"journal":{"name":"Microbial Cell","volume":"13 ","pages":"28-43"},"PeriodicalIF":3.9,"publicationDate":"2026-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12867487/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146119444","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transcriptomic response to different heme sources in Trypanosoma cruzi epimastigotes. 克氏锥虫对不同血红素来源的转录组学响应。

IF 3.9 3区生物学

Microbial Cell Pub Date : 2026-01-23 eCollection Date: 2026-01-01 DOI: 10.15698/mic2026.01.865

Evelyn Tevere, María G Mediavilla, Cecilia B Di Capua, Marcelo L Merli, Carlos Robello, Luisa Berná, Julia A Cricco

{"title":"Transcriptomic response to different heme sources in Trypanosoma cruzi epimastigotes.","authors":"Evelyn Tevere, María G Mediavilla, Cecilia B Di Capua, Marcelo L Merli, Carlos Robello, Luisa Berná, Julia A Cricco","doi":"10.15698/mic2026.01.865","DOIUrl":"10.15698/mic2026.01.865","url":null,"abstract":"Heme is an essential molecule for most organisms, yet some parasites, like Trypanosoma cruzi, the causative agent of Chagas disease, cannot synthesize it. These parasites must acquire heme from their hosts, making this process critical for their survival. In the midgut of the insect vector, T. cruzi epimastigotes are exposed to both hemoglobin (Hb) and free heme resulting from its degradation. Despite the importance of this nutrient, how different heme sources influence parasite gene expression remains poorly understood. Here, we showed that heme restitution either as hemin or Hb to heme-starved parasites induces an early and distinct transcriptional response in T. cruzi epimastigotes. Using RNA sequencing at 4- and 24-hours post-supplementation, we identified gene subsets commonly or uniquely regulated by each heme source, including genes putatively linked to heme acquisition and metabolism. The study includes the first focused characterization of CRAL/TRIO domain-containing protein (TcCRAL/TRIO), a novel heme-responsive hemoprotein. Our results provide a more detailed picture of T. cruzi biology and highlights heme acquisition as a promising point of vulnerability to control parasite proliferation.","PeriodicalId":18397,"journal":{"name":"Microbial Cell","volume":"13 ","pages":"13-27"},"PeriodicalIF":3.9,"publicationDate":"2026-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12834515/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146064564","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sir2 regulates selective autophagy in stationary-phase yeast cells. Sir2调控静止期酵母细胞的选择性自噬。

IF 3.9 3区生物学

Microbial Cell Pub Date : 2026-01-19 eCollection Date: 2026-01-01 DOI: 10.15698/mic2026.01.864

Ji-In Ryu, Juhye Jung, Jeong-Yoon Kim

{"title":"Sir2 regulates selective autophagy in stationary-phase yeast cells.","authors":"Ji-In Ryu, Juhye Jung, Jeong-Yoon Kim","doi":"10.15698/mic2026.01.864","DOIUrl":"10.15698/mic2026.01.864","url":null,"abstract":"Autophagy contributes to cellular homeostasis by degrading and recycling intracellular components, especially under nutrient-limited conditions. While autophagy is well characterized under acute starvation in synthetic media in Saccharomyces cerevisiae, its regulation during the stationary phase of prolonged growth in nutrient-rich complex media, when cells experience gradual metabolic shifts and sustained stress, remains poorly understood. In this study, we identified Sir2, an NAD <math><msup><mrow></mrow> <mrow><mo>+</mo></mrow> </msup> </math> -dependent histone deacetylase, as a key suppressor of autophagy during the stationary phase in YPD complex medium. Using GFP-Atg8 processing as a readout of autophagic flux, we demonstrated that SIR2 deletion led to sustained autophagy activation. Notably, Sir2 selectively inhibited mitophagy, pexophagy, and the Cvt pathway, while non-selective autophagy remained largely unaffected. Transcriptomic analysis revealed that Sir2 facilitates a coordinated entry into quiescence, in part by regulating ribosome biogenesis and nutrient-responsive pathways during the stationary phase. Mechanistically, Sir2 stabilized Ume6, a repressor of ATG8 transcription, thereby limiting autophagic activity. Deletion of SIR2 drastically increased the phosphorylation and stabilization of the mitochondrial receptor Atg32 during the stationary phase, leading to enhanced mitophagy. Additionally, we found that ROS generated by mitophagy enhanced autophagy through a positive feedback loop. Collectively, our findings establish Sir2 as a previously unrecognized regulator of selective autophagy during the stationary phase in complex medium and highlight how cells dynamically control organelle degradation to maintain viability under extended metabolic stress.","PeriodicalId":18397,"journal":{"name":"Microbial Cell","volume":"13 ","pages":"1-12"},"PeriodicalIF":3.9,"publicationDate":"2026-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12826798/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146052957","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Luminal acetylation of microtubules is not essential for Plasmodium berghei and Toxoplasma gondii survival. 微管的腔内乙酰化对伯氏疟原虫和刚地弓形虫的存活并不是必需的。

IF 3.9 3区生物学

Microbial Cell Pub Date : 2025-12-17 eCollection Date: 2025-01-01 DOI: 10.15698/mic2025.12.863

Thrishla Kumar, Katharina Röver, Johannes F Stortz, Annika M Binder, Benjamin Spreng, Madlen Konert, Markus Meissner, Friedrich Frischknecht, Elena Jimenez-Ruiz

{"title":"Luminal acetylation of microtubules is not essential for Plasmodium berghei and Toxoplasma gondii survival.","authors":"Thrishla Kumar, Katharina Röver, Johannes F Stortz, Annika M Binder, Benjamin Spreng, Madlen Konert, Markus Meissner, Friedrich Frischknecht, Elena Jimenez-Ruiz","doi":"10.15698/mic2025.12.863","DOIUrl":"10.15698/mic2025.12.863","url":null,"abstract":"Post-translational modifications of microtubules regulate their stability and dynamics. Acetylation of <math><mi>α</mi></math> tubulin at lysine 40 (K40) by <math><mi>α</mi></math> -acetyltransferase ( <math><mi>α</mi></math> TAT) occurs on the luminal side of microtubules, stabilizes their structure, and plays essential roles in various cellular processes across eukaryotes. Apicomplexan parasites include the malaria-causing Plasmodium species and Toxoplasma gondii, both of which possess unusually stable subpellicular microtubules, a set of cytoskeletal filaments underlying the parasite's inner membrane complex. Interestingly, while Toxoplasma gondii and human-infecting Plasmodium species retain both K40 and <math><mi>α</mi></math> TAT, rodent-infecting Plasmodium species have lost <math><mi>α</mi></math> TAT, and K40 has been replaced by glutamine (Q40), a residue that can mimic acetylated lysine. Here, we investigate the role of microtubule acetylation in apicomplexan parasites by generating and characterizing genetic mutants in Plasmodium berghei and Toxoplasma gondii. In Plasmodium berghei, introduction of a Q40K mutation in <math><mi>α</mi></math> 1 tubulin did not affect parasite development or infectivity, suggesting that the absence of K40 acetylation is not detrimental. In Toxoplasma gondii, we confirmed that <math><mi>α</mi></math> TAT is responsible for microtubule acetylation but, contrary to previous reports, its deletion had no impact on parasite growth in vitro. Together, these results indicate that luminal K40 acetylation is not essential for microtubule function in either species, pointing to functional redundancy and highlighting the plasticity of cytoskeletal regulation in apicomplexan parasites.","PeriodicalId":18397,"journal":{"name":"Microbial Cell","volume":"12 ","pages":"299-313"},"PeriodicalIF":3.9,"publicationDate":"2025-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12795560/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145966342","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The dual-site agonist for human M2 muscarinic receptors Iper-8-naphtalimide induces mitochondrial dysfunction in Saccharomyces cerevisiae. 人M2毒蕈碱受体双位点激动剂iper -8-萘酰亚胺诱导酿酒酵母线粒体功能障碍。

IF 3.9 3区生物学

Microbial Cell Pub Date : 2025-12-12 eCollection Date: 2025-01-01 DOI: 10.15698/mic2025.12.862

Angela Cirigliano, Antonia Amelina, Elena Passarini, Alessandra Ricelli, Nicole Balasco, Mattia Mori, Bruno Botta, Maria Egle De Stefano, Claudio Papotto, Claudia Guerriero, Ada Maria Tata, Teresa Rinaldi

{"title":"The dual-site agonist for human M2 muscarinic receptors Iper-8-naphtalimide induces mitochondrial dysfunction in Saccharomyces cerevisiae.","authors":"Angela Cirigliano, Antonia Amelina, Elena Passarini, Alessandra Ricelli, Nicole Balasco, Mattia Mori, Bruno Botta, Maria Egle De Stefano, Claudio Papotto, Claudia Guerriero, Ada Maria Tata, Teresa Rinaldi","doi":"10.15698/mic2025.12.862","DOIUrl":"10.15698/mic2025.12.862","url":null,"abstract":"Glioblastoma is a malignant astrocytic tumor of the brain. A significantly decrease of glioblastoma cell proliferation and survival can be achieved by activating the M2 muscarinic acetylcholine receptor (a G protein-coupled receptor, or GPCR) with two agonist molecules, the orthosteric agonist Arecaidine Propargyl Ester (APE) and the dual-steric agonist Iper-8-naphthalimide (N-8-Iper). In glioblastoma cells, these agonists caused mitochondrial damage and an altered lipid profile. To characterize the mitochondrial dysfunction induced by the muscarinic agonists, we tested APE and N-8-Iper in S. cerevisiae, a yeast model system specifically suitable to study the activity of molecules of pharmaceutical interest on mitochondria. N-8-Iper, but not APE, induced mitochondrial dysfunction in S. cerevisiae cells in a time- and concentration-dependent manner. These results suggest that the agonist N-8-Iper on glioblastoma cell cultures has a direct effect on mitochondrial function. Moreover, since GPCRs are evolutionarily conserved from yeast to humans, these results confirm that the yeast system is a suitable model for studying human GPCRs.","PeriodicalId":18397,"journal":{"name":"Microbial Cell","volume":"12 ","pages":"290-298"},"PeriodicalIF":3.9,"publicationDate":"2025-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12713414/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145804961","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The core genetic drivers of chronological aging in yeast are universal regulators of longevity. 酵母中按时间顺序衰老的核心遗传驱动因素是长寿的普遍调节因子。

IF 3.9 3区生物学

Microbial Cell Pub Date : 2025-10-31 eCollection Date: 2025-01-01 DOI: 10.15698/mic2025.10.861

Erika Cruz-Bonilla, Sergio E Campos, Soledad Funes, Cei Abreu-Goodger, Alexander DeLuna

{"title":"The core genetic drivers of chronological aging in yeast are universal regulators of longevity.","authors":"Erika Cruz-Bonilla, Sergio E Campos, Soledad Funes, Cei Abreu-Goodger, Alexander DeLuna","doi":"10.15698/mic2025.10.861","DOIUrl":"10.15698/mic2025.10.861","url":null,"abstract":"The chronological lifespan of Saccharomyces cerevisiae has significantly contributed to our understanding of aging in eukaryotic cells. However, gaining a genome-wide perspective of this trait remains challenging due to substantial discrepancies observed across genome-wide gene-deletion screens. In this study, we systematically compiled nine chronological-lifespan datasets and evaluated how shared experimental variables influenced screen variability. Furthermore, we performed a meta-analysis to compile a ranked catalog of key processes and regulators driving chronological longevity in yeast, ensuring their robustness across diverse experimental setups. These consistent chronological aging factors were enriched in genes associated with yeast replicative lifespan and orthologs implicated in aging across other model organisms. Functional analysis revealed that the downstream cellular mechanisms underlying chronological longevity in yeast align with well-established, universal hallmarks of aging. Importantly, we identified transcriptional regulators associated with these consistent genetic factors, uncovering potential global and local modulators of chronological aging. Our findings provide an integrated view of the core genetic landscape underlying aging in yeast, highlighting the value of the chronological lifespan paradigm for investigating conserved mechanisms of aging.","PeriodicalId":18397,"journal":{"name":"Microbial Cell","volume":"12 ","pages":"274-289"},"PeriodicalIF":3.9,"publicationDate":"2025-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12632094/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145588157","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Organelle activity organized by the endoplasmic reticulum-mitochondria encounter structure -ERMES- is essential for Podospora anserina development. 由内质网-线粒体相遇结构（ermes）组织的细胞器活动对鹅足孢的发育至关重要。

IF 3.9 3区生物学

Microbial Cell Pub Date : 2025-09-12 eCollection Date: 2025-01-01 DOI: 10.15698/mic2025.09.860

Melisa Álvarez-Sánchez, Matías Ramírez-Noguez, Beatriz Aguirre-López, Leonardo Peraza-Reyes

{"title":"Organelle activity organized by the endoplasmic reticulum-mitochondria encounter structure -ERMES- is essential for Podospora anserina development.","authors":"Melisa Álvarez-Sánchez, Matías Ramírez-Noguez, Beatriz Aguirre-López, Leonardo Peraza-Reyes","doi":"10.15698/mic2025.09.860","DOIUrl":"10.15698/mic2025.09.860","url":null,"abstract":"Eucaryotic cell functioning and development depend on the concerted activity of its organelles. In the model fungus Podospora anserina, sexual development involves a dynamic regulation of mitochondria, peroxisomes and the endoplasmic reticulum (ER), suggesting that their activity during this process is coordinated. The ER-Mitochondria Encounter Structure (ERMES) is a tether complex composed of the ER protein Mmm1 and the mitochondrial proteins Mdm10, Mdm12 and Mdm34, which mediates membrane contact-site formation between these organelles. This complex also mediates interactions between mitochondria and peroxisomes. Here we analyzed the role of the ERMES complex during P. anserina development. By studying a thermosensitive MDM10 mutant, we show that MDM10 is required for mitochondrial morphology and distribution, as well as for peroxisome membrane-remodeling and motility. We discovered that lipid droplets exhibit a subapical hyphal localization, which depends on MDM10. MDM10 is also required for ER shaping and dynamics, notably of the apical ER domains of the polarized-growing hyphal region, in a process that involves the activity of the protein YOP1. We also show that apical ER shaping involves a Spitzenkörper-associated membrane traffic, which implicates MDM10, and that the mycelial growth defect of mdm10 mutants is exacerbated when the ER-shaping proteins YOP1 or RTN1 are loss. Finaly, we show that MMM1 is strictly required for mycelial growth and sexual development, suggesting that its activity is essential. Our results show that the activity of distinct organelles depends on the ERMES complex, and that the function of this complex is critical for P. anserina growth and development.","PeriodicalId":18397,"journal":{"name":"Microbial Cell","volume":"12 ","pages":"255-273"},"PeriodicalIF":3.9,"publicationDate":"2025-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12427119/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145065123","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The molecular mechanisms and physiological roles of mitochondria dynamics in Saccharomyces cerevisiae. 酿酒酵母线粒体动力学的分子机制及其生理作用。

IF 3.9 3区生物学

Microbial Cell Pub Date : 2025-08-27 eCollection Date: 2025-01-01 DOI: 10.15698/mic2025.08.859

Chang-Lin Chen, Wei-Ling Huang, Alexander Rapoport, Rimantas Daugelavičius, Chuang-Rung Chang

{"title":"The molecular mechanisms and physiological roles of mitochondria dynamics in Saccharomyces cerevisiae.","authors":"Chang-Lin Chen, Wei-Ling Huang, Alexander Rapoport, Rimantas Daugelavičius, Chuang-Rung Chang","doi":"10.15698/mic2025.08.859","DOIUrl":"10.15698/mic2025.08.859","url":null,"abstract":"Mitochondria are essential organelles that form a dynamic network within cells. The fusion, fission, and transport processes among mitochondria must reach a balance, which is achieved through complex regulatory mechanisms. These dynamic processes and regulatory pathways are highly conserved across species and are coordinated to help cells respond to environmental stress. The budding yeast Saccharomyces cerevisiae has become an important model organism for studying mitochondria dynamics due to its genetic tractability and the conservation of key mitochondrial regulators. Previous research on mitochondria dynamics in yeast has provided valuable insights into the regulatory pathways in eukaryotic cells. It has helped to elucidate the mechanisms related to diseases associated with disrupted mitochondria dynamics. This review explores the molecular mechanisms underlying mitochondria dynamics and their physiological roles in Saccharomyces cerevisiae. The knowledge we learned from the primary eukaryotic yeast cell will aid us in advancing future research on the regulatory mechanisms of mitochondria in both health and disease.","PeriodicalId":18397,"journal":{"name":"Microbial Cell","volume":"12 ","pages":"242-254"},"PeriodicalIF":3.9,"publicationDate":"2025-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12407545/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145000919","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0