Medical Molecular Morphology最新文献_第8页

Expression of CILP-2 and DDR2 and ultrastructural changes in the articular cartilage of patients with knee osteoarthritis undergoing total knee arthroplasty: a pilot morphological study. 全膝关节置换术后膝关节骨性关节炎患者关节软骨中CILP-2和DDR2的表达及超微结构变化:一项初步形态学研究

IF 1.8 4区医学

Medical Molecular Morphology Pub Date : 2023-03-01 DOI: 10.1007/s00795-022-00339-4

Taavi Torga, Siim Suutre, Kalle Kisand, Marina Aunapuu, Andres Arend

{"title":"Expression of CILP-2 and DDR2 and ultrastructural changes in the articular cartilage of patients with knee osteoarthritis undergoing total knee arthroplasty: a pilot morphological study.","authors":"Taavi Torga, Siim Suutre, Kalle Kisand, Marina Aunapuu, Andres Arend","doi":"10.1007/s00795-022-00339-4","DOIUrl":"https://doi.org/10.1007/s00795-022-00339-4","url":null,"abstract":"The aim of the study was to correlate the immunohistochemical expression of cartilage intermediate layer protein 2 (CILP-2) and discoidin domain receptor 2 (DDR2), and the ultrastructural changes in the cartilage with the degree of articular cartilage damage in osteoarthritis (OA) patients. Cartilage samples were obtained from twenty patients aged from 46 to 68 years undergoing total knee arthroplasty. In each patient, medial and lateral tibial plateau samples were analysed applying OARSI histopathology grading. Positive correlation was noted between the extent of CILP-2 staining intensity and OARSI grades. Abundant staining for CILP-2 was found in the superficial and middle layers and in the pericellular matrix (PCM) of the deep zone. Transmission electron microscopy studies demonstrated strong damage of chondrocytes, the organelles were often diminished or focally aggregated. As a characteristic finding, PCM was frequently expanded, which may reflect a pathogenic step in OA progression. In conclusion, CILP-2 may potentially be a relevant marker of OA progression as its expression correlated better with cartilage damage than the known marker of articular cartilage damage, DDR2.","PeriodicalId":18338,"journal":{"name":"Medical Molecular Morphology","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10757301","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Atypical spindle cell/pleomorphic lipomatous tumor with a sarcomatous component showing high mitotic activity and Ki-67 labeling index: report of a unique case mimicking dedifferentiated liposarcoma. 具有高有丝分裂活性和Ki-67标记指数的肉瘤成分的非典型梭形细胞/多形性脂肪瘤:报告一例模拟去分化脂肪肉瘤的独特病例。

IF 1.8 4区医学

Medical Molecular Morphology Pub Date : 2022-12-01 Epub Date: 2022-07-02 DOI: 10.1007/s00795-022-00327-8

Shintaro Sugita, Taro Sugawara, Makoto Emori, Tomoyuki Aoyama, Michiko Hosaka, Keiko Segawa, Hiromi Fujita, Tadashi Hasegawa

{"title":"Atypical spindle cell/pleomorphic lipomatous tumor with a sarcomatous component showing high mitotic activity and Ki-67 labeling index: report of a unique case mimicking dedifferentiated liposarcoma.","authors":"Shintaro Sugita, Taro Sugawara, Makoto Emori, Tomoyuki Aoyama, Michiko Hosaka, Keiko Segawa, Hiromi Fujita, Tadashi Hasegawa","doi":"10.1007/s00795-022-00327-8","DOIUrl":"https://doi.org/10.1007/s00795-022-00327-8","url":null,"abstract":"Atypical spindle cell/pleomorphic lipomatous tumor (ASPLT) is a new entity of benign adipocytic tumor that spans a wide spectrum of histology from adipocytic to spindle cell/pleomorphic tumors. The latter non-adipocytic component rarely shows sarcomatous features although ASPLTs are not thought to dedifferentiate. A 78-year-old woman with ASPLT in the left thigh had a sarcomatous component with high mitotic activity and Ki-67 labeling index (LI) mimicking dedifferentiated liposarcoma. The adipocytic component consisted of various-sized adipocytic cells with few lipoblasts. The sarcomatous component consisted of a fascicular proliferation of atypical spindle cells with scattered large bizarre and multinucleated giant cells. Mitotic figures including atypical mitoses were frequently observed. Immunohistochemically, the tumor cells were positive for cluster of differentiation 34 but not mouse double minute 2 homolog (MDM2), cyclin-dependent kinase 4 (CDK4), or retinoblastoma (Rb) protein. Ki-67 LI in the sarcomatous component reached 40%. MDM2 and CDK4 genes were not amplified and 13q14 including the RB1 locus was deleted according to fluorescence in situ hybridization. The patient is alive with no evidence of local recurrence or distant metastasis 3.5 years after surgery. As ASPLT may exhibit morphological variation, it is important to rule out dedifferentiated liposarcoma with careful pathological examination.","PeriodicalId":18338,"journal":{"name":"Medical Molecular Morphology","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40472668","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

A symptomatic intercalated duct lesion of the parotid gland: a case report with immunohistochemical and genetic analyses. 腮腺有症状的插层导管病变:附免疫组织化学及基因分析1例。

IF 1.8 4区医学

Medical Molecular Morphology Pub Date : 2022-12-01 Epub Date: 2022-07-05 DOI: 10.1007/s00795-022-00328-7

Kimihide Kusafuka, Satoshi Baba, Yoshiharu Kitani, Kazuki Hirata, Akinori Murakami, Aya Muramatsu, Kazumori Arai, Makoto Suzuki

{"title":"A symptomatic intercalated duct lesion of the parotid gland: a case report with immunohistochemical and genetic analyses.","authors":"Kimihide Kusafuka, Satoshi Baba, Yoshiharu Kitani, Kazuki Hirata, Akinori Murakami, Aya Muramatsu, Kazumori Arai, Makoto Suzuki","doi":"10.1007/s00795-022-00328-7","DOIUrl":"https://doi.org/10.1007/s00795-022-00328-7","url":null,"abstract":"Intercalated duct lesions (IDLs) are usually asymptomatic. We report a case of IDL, in which a palpable mass formed. The patient was a 45-year-old Japanese male, who noticed a mass in the left parotid region. The nodular lesion was well-circumscribed, but did not have a fibrous capsule or exhibit infiltrative growth. It contained a small cystic space and consisted of basaloid cells arranged in a cribriform pattern and inner ductal cells. It had some solid areas of nest-like proliferation displaying mild cellular atypia. Immunohistochemically, the luminal cells were positive for cytokeratin (CK)7 and epithelial membrane antigen, and the abluminal cells were positive for CK5/6, p63, and DOG1. S-100 protein-positive stromal cells were also seen. The lesion's cells were all positive for SOX10, and the nuclei of some basaloid cells were positive for β-catenin. The Ki-67 labeling index was 3.8%. The ductal cells contained diastase-digestion-resistant, Periodic acid Schiff-positive zymogen granules. Genetically, the lesion harbored a missense mutation in the CTNNB1 gene. We diagnosed the lesion as an IDL. As IDLs are usually small non-neoplastic lesions, symptomatic cases are rare. Based on its common immunohistochemical and genetic features, IDL may be a precursor of basal cell adenoma/adenocarcinoma, such as intercalated duct adenoma.","PeriodicalId":18338,"journal":{"name":"Medical Molecular Morphology","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40584740","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Iris-derived induced pluripotent stem cells that express GFP in all somatic cells of mice and differentiate into functional retinal neurons. 在小鼠所有体细胞中表达GFP并分化为功能性视网膜神经元的虹膜来源诱导多能干细胞。

IF 1.8 4区医学

Medical Molecular Morphology Pub Date : 2022-12-01 Epub Date: 2022-08-06 DOI: 10.1007/s00795-022-00330-z

Noriko Hiramatsu, Naoki Yamamoto, Mahito Ohkuma, Noriaki Nagai, Ei-Ichi Miyachi, Kumiko Yamatsuta, Kazuyoshi Imaizumi

引用次数: 0

Hepatic expression of sodium-glucose cotransporter 2 (SGLT2) in patients with chronic liver disease. 慢性肝病患者钠-葡萄糖共转运蛋白2 (SGLT2)在肝脏中的表达

IF 1.8 4区医学

Medical Molecular Morphology Pub Date : 2022-12-01 Epub Date: 2022-09-21 DOI: 10.1007/s00795-022-00334-9

Dan Nakano, Jun Akiba, Tsubasa Tsutsumi, Machiko Kawaguchi, Takafumi Yoshida, Hironori Koga, Takumi Kawaguchi

{"title":"Hepatic expression of sodium-glucose cotransporter 2 (SGLT2) in patients with chronic liver disease.","authors":"Dan Nakano, Jun Akiba, Tsubasa Tsutsumi, Machiko Kawaguchi, Takafumi Yoshida, Hironori Koga, Takumi Kawaguchi","doi":"10.1007/s00795-022-00334-9","DOIUrl":"https://doi.org/10.1007/s00795-022-00334-9","url":null,"abstract":"Sodium-glucose cotransporter 2 (SGLT2) occurs in the proximal renal tubule cells. We investigate the hepatic expression of SGLT2 and its related factors in patients with chronic liver disease. This is a retrospective human study. The liver tissues were biopsied from patients with chronic liver disease (n = 30). The expression levels of SGLT2 were evaluated by immunostaining. Furthermore, the undirected graphical model was used to identify factors associated with hepatic expression levels of SGLT2. The SGLT2 expression was observed in not only the kidney, but also the liver in immunostaining (SGLT2 intensity: kidney 165.8 ± 15.6, liver 114.4 ± 49.0 arbitrary units, P < 0.01) and immunoblotting. There was no significant difference in hepatic expression of SGLT2 in the stratified analysis according to age, sex, BMI, and the severity of the liver disease. In the undirected graphical model, SGLT2 directly interacted with various factors such as sex, fatty change, neutrophil-to-lymphocyte ratio, triglyceride, hemoglobin A1c, creatinine, and albumin (partial correlation coefficient 0.4-0.6 for sex and 0.2-0.4 for others). The expression of SGLT2 was observed in the hepatocytes of patients with chronic liver disease. The undirected graphical model demonstrated the complex interaction of hepatic expression levels of SGLT2 with gender, inflammation, renal function, and lipid/glucose/protein metabolisms.","PeriodicalId":18338,"journal":{"name":"Medical Molecular Morphology","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9606064/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40374689","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Pathological classification of desmoplastic reaction is prognostic factor in cervical adenocarcinoma. 结缔组织增生反应的病理分型是影响子宫颈腺癌预后的因素。

IF 1.8 4区医学

Medical Molecular Morphology Pub Date : 2022-12-01 Epub Date: 2022-07-25 DOI: 10.1007/s00795-022-00329-6

Taishi Akimoto, Akira Takasawa, Kumi Takasawa, Tomoyuki Aoyama, Motoki Matsuura, Masato Tamate, Masahiro Iwasaki, Shutaro Habata, Taro Murakami, Makoto Osanai, Tsuyoshi Saito

{"title":"Pathological classification of desmoplastic reaction is prognostic factor in cervical adenocarcinoma.","authors":"Taishi Akimoto, Akira Takasawa, Kumi Takasawa, Tomoyuki Aoyama, Motoki Matsuura, Masato Tamate, Masahiro Iwasaki, Shutaro Habata, Taro Murakami, Makoto Osanai, Tsuyoshi Saito","doi":"10.1007/s00795-022-00329-6","DOIUrl":"https://doi.org/10.1007/s00795-022-00329-6","url":null,"abstract":"Desmoplastic reaction (DR) and inflammation are significant pathological manifestations of tumorigenesis in several cancers. However, the correlation between these stromal reactions and cervical adenocarcinoma has been poorly documented. This investigation elucidated whether DR is a prognostic indicator in early cervical adenocarcinoma patients. Fifty-nine patients with early stage cervical adenocarcinoma (stages I/II) were included in the study. DR was divided into three groups, mature, intermediate, and immature, based on the presence of myxoid stroma and hyalinized keloid-like collagen. Inflammatory cell responses were classified as mild, moderate, and severe. Those stromal reactions were separately evaluated in the invasion front stroma and intratumoral stroma. In both the intratumor and invasion front stroma, intermediate/immature DR was correlated with tumor size, T stage, N stage, lymphovascular invasion, and parametrial infiltration (p < 0.001 to p < 0.05). In addition, in the intratumoral stroma, intermediate/immature DR led to short relapse-free survival and overall survival (p < 0.001). In the invasion front stroma, inflammatory cell responses were associated with DR immaturity and FIGO stage (p < 0.01). These results suggest that the classification of DR maturity is a potential prognostic biomarker in early stage cervical adenocarcinoma patients. DR can be evaluated by routine H&E staining without immunohistochemistry, making it convenient and economical in clinical practice.","PeriodicalId":18338,"journal":{"name":"Medical Molecular Morphology","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40623926","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Expression and clinicopathological significance of glucocorticoid receptor, SGK1, and NDRG1 in hormone-naïve prostate carcinoma. 糖皮质激素受体SGK1、NDRG1在hormone-naïve前列腺癌中的表达及临床病理意义。

IF 1.8 4区医学

Medical Molecular Morphology Pub Date : 2022-12-01 Epub Date: 2022-07-21 DOI: 10.1007/s00795-022-00332-x

Shuko Hata, Hiroki Shimada, Naomi Sato, Mayu Koshiishi, Kazue Ise, Tomoaki Ogata, Shinichi Yamashita, Akihiro Ito, Hironobu Sasano, Yasuhiro Nakamura

{"title":"Expression and clinicopathological significance of glucocorticoid receptor, SGK1, and NDRG1 in hormone-naïve prostate carcinoma.","authors":"Shuko Hata, Hiroki Shimada, Naomi Sato, Mayu Koshiishi, Kazue Ise, Tomoaki Ogata, Shinichi Yamashita, Akihiro Ito, Hironobu Sasano, Yasuhiro Nakamura","doi":"10.1007/s00795-022-00332-x","DOIUrl":"https://doi.org/10.1007/s00795-022-00332-x","url":null,"abstract":"Glucocorticoid receptor (GR) has been implicated in prostate carcinoma growth and progression. Glucocorticoid receptor beta (GRβ) acts as an inhibitor of GR; however, its function is not well understood. Serum- and glucocorticoid-regulated kinase 1 (SGK1) is a GR-responsive gene that phosphorylates N-myc downstream-regulated gene 1 (NDRG1) and is involved in cancer growth and invasion. However, the expression of GR, GRβ, SGK1, and NDRG1 in prostate cancer and their relationship with clinicopathological and functional significance remain unknown. The association between the status of GR, GRβ, SGK1, and NDRG1 immunoreactivity and clinicopathological variables was analyzed in patients with prostate carcinoma to explore their clinical significance. In prostate carcinoma cases, the relative abundance of GR and NDRG1 immunoreactivity was inversely and significantly associated with the primary tumor stage (pT), while GR immunoreactivity was inversely and significantly associated with the Ki-67 score. The relative expression status of NDRG1 was significantly associated with that of GR. However, no significant correlation was observed between any of the clinicopathological parameters and GRβ and SGK1 expression. Our findings indicate that GR and NDRG1 expression status is correlated with clinicopathological features in patients with prostate cancer.","PeriodicalId":18338,"journal":{"name":"Medical Molecular Morphology","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40542095","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Sputum characteristics of patients with severe COVID-19: report of two cases with immunocytochemical detection of SARS-CoV-2 spike protein. 重症COVID-19患者痰液特征:2例SARS-CoV-2刺突蛋白免疫细胞化学检测报告

IF 1.8 4区医学

Medical Molecular Morphology Pub Date : 2022-12-01 Epub Date: 2022-06-18 DOI: 10.1007/s00795-022-00326-9

Daichi Fujimoto, Minako Fukuya, Sachie Terao, Isao Irei, Takashi Akiyama, Anna Watanabe, Yuri Yasuda, Daisuke Yoshioka, Kazuhide Takada, Satoshi Hayakawa, Takuya Moriya

{"title":"Sputum characteristics of patients with severe COVID-19: report of two cases with immunocytochemical detection of SARS-CoV-2 spike protein.","authors":"Daichi Fujimoto, Minako Fukuya, Sachie Terao, Isao Irei, Takashi Akiyama, Anna Watanabe, Yuri Yasuda, Daisuke Yoshioka, Kazuhide Takada, Satoshi Hayakawa, Takuya Moriya","doi":"10.1007/s00795-022-00326-9","DOIUrl":"https://doi.org/10.1007/s00795-022-00326-9","url":null,"abstract":"Patients with SARS-CoV-2 infection and with severe COVID-19 often have multiple coinfections, and their treatment is challenging. Here, we performed cytology analysis on sputum samples from two patients with severe COVID-19. The specimens were prepared using the rubbing method and stained with Papanicolaou stain. In both cases, several cells with frosted nuclei were observed, and the cytological findings per 100 cells were evaluated. The infected cells were mononuclear to multinuclear, showing chromatin aggregation at the nuclear margins, intranuclear inclusion bodies, eosinophilic cytoplasmic inclusion bodies, and mutual pressure exclusion of the nuclei. Immunocytochemical staining revealed that the cells were positive for AE1/AE3 and negative for CD68 expression, indicating their epithelial origin. Furthermore, infected cells with frosted nuclei were positive for surfactant protein A (SP-A) in Case 2, suggesting infection of type II alveolar pneumocytes or Clara cells. Moreover, in Case 2, the infected cells were positive for herpes simplex virus (HSV) I + II and SARS-CoV-2 spike protein, confirming double infection in these cells. In conclusion, sputum cytology is an important tool for determining the diversity of viral infection, and additional immunocytochemistry can be used for definitive diagnosis.","PeriodicalId":18338,"journal":{"name":"Medical Molecular Morphology","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9206128/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39986487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Pancreatic cancer cell-derived exosomes induce epithelial-mesenchymal transition in human pancreatic cancer cells themselves partially via transforming growth factor β1. 胰腺癌细胞衍生的外泌体部分通过转化生长因子β1诱导人胰腺癌细胞的上皮-间质转化。

IF 1.8 4区医学

Medical Molecular Morphology Pub Date : 2022-09-01 Epub Date: 2022-04-27 DOI: 10.1007/s00795-022-00321-0

Fumiya Nakayama, Makoto Miyoshi, Ai Kimoto, Akari Kawano, Kumiko Miyashita, Shingo Kamoshida, Kazuya Shimizu, Yuichi Hori

{"title":"Pancreatic cancer cell-derived exosomes induce epithelial-mesenchymal transition in human pancreatic cancer cells themselves partially via transforming growth factor β1.","authors":"Fumiya Nakayama, Makoto Miyoshi, Ai Kimoto, Akari Kawano, Kumiko Miyashita, Shingo Kamoshida, Kazuya Shimizu, Yuichi Hori","doi":"10.1007/s00795-022-00321-0","DOIUrl":"10.1007/s00795-022-00321-0","url":null,"abstract":"Distant metastasis is a dismal prognostic factor of pancreatic cancer. Metastasis is established in several steps, but the mechanism underlying the very early stages remains unclear. Epithelial-mesenchymal transition (EMT) is involved in these stages. Although signaling molecules have been reported to induce EMT, the mechanism underlying their origin is unclear. In this study, we hypothesized that pancreatic cancer cell-derived exosomes induce EMT in cancer cells themselves, a notion we entertained because we found EMT in in vitro three-dimensional colonies of cancer cells, with vimentin-positive cells observed in some of the budding pancreatic cancer cells and in single cells outside the colony as well. First, we clarified that pancreatic cancer cell-derived exosomes induce EMT in cancer cells themselves. Next, we examined the involvement of transforming growth factor-β1 (TGF-β1), and TGF-β1 knock-down in pancreatic cancer cells with TGF-β1 siRNA significantly suppressed TGF-β1 gene expression in cancer cells, and exosomal TGF-β1 was significantly reduced in the secretory exosomes. Exosomes from TGF-β1 knock-down cells suppressed EMT induction in cancer cells themselves and TGF-β1 protein expression in target cells. Taken together, these findings suggest that TGF-β1 is involved in EMT induction via exosomes, results that may support the production of effective metastasis inhibitors.","PeriodicalId":18338,"journal":{"name":"Medical Molecular Morphology","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9043512/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81716479","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Significance of the association between early embryonic development and endocytosis. 早期胚胎发育与内吞作用关系的意义。

IF 1.8 4区医学

Medical Molecular Morphology Pub Date : 2022-09-01 Epub Date: 2022-07-14 DOI: 10.1007/s00795-022-00331-y

Akihito Morita, Yuhkoh Satouh, Ken Sato, Akira Iwase

{"title":"Significance of the association between early embryonic development and endocytosis.","authors":"Akihito Morita, Yuhkoh Satouh, Ken Sato, Akira Iwase","doi":"10.1007/s00795-022-00331-y","DOIUrl":"https://doi.org/10.1007/s00795-022-00331-y","url":null,"abstract":"Fertilization triggers a process called maternal-to-zygotic transition, in which the oocyte undergoes oocyte-to-embryo transition, leading to massive intracellular remodeling toward early embryogenesis. This transition requires the degradation of oocyte-derived components; however, the significance and mechanism of degradation of cell surface components remain unknown. In this review, we focused on the dynamics of plasma membrane proteins and investigated the relationship between embryonic development and endocytosis. Our survey of the extant literature on the topic led to the conclusion that clathrin-mediated endocytosis is essential for the progression of early embryogenesis and selective degradation of oocyte-derived plasma membrane proteins in mouse embryos, as reported by studies analyzing maternal cellular surface proteins, including a glycine transporter, GlyT1a. Evaluation of such endocytic activity in individual embryos may allow the selection of embryos with higher viability in assisted reproductive technologies, and it is important to select viable embryos to increase the rates of successful pregnancy and live birth. Although the early embryonic developmental abnormalities are mainly accompanied with chromosomal aneuploidy, other causes and mechanisms remain unclear. This review summarizes molecular biological approaches to early embryonic developmental abnormalities and their future prospects.","PeriodicalId":18338,"journal":{"name":"Medical Molecular Morphology","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40602923","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1