Medical Molecular Morphology最新文献_第8页

Suppressed distribution of protein A on the surface of Staphylococcus aureus as a morphological characteristic of erythromycin-resistant strain 金黄色葡萄球菌表面蛋白 A 的分布抑制是耐红霉素菌株的形态特征之一

IF 1.8 4区医学

Medical Molecular Morphology Pub Date : 2024-02-22 DOI: 10.1007/s00795-023-00379-4

{"title":"Suppressed distribution of protein A on the surface of Staphylococcus aureus as a morphological characteristic of erythromycin-resistant strain","authors":"","doi":"10.1007/s00795-023-00379-4","DOIUrl":"https://doi.org/10.1007/s00795-023-00379-4","url":null,"abstract":"<h3>Abstract</h3> To identify a new morphological phenotype of erythromycin (EM)-resistant Staphylococcus aureus (S. aureus) were isolated in vitro from EM-sensitive parent strain, and the distribution of staphylococcus specific protein A (SpA) on the surface of these strains was examined morphologically by using applied immunoelectron microscopy. The isolated EM-resistant strains had thickened cell walls, and the distribution of SpA on the surfaces of these strains was demonstrated to be lower than that of the parent strain. The SpA suppression was confirmed by enzyme-linked immunosorbent assay (ELISA) using fixed EM-resistant cells. Moreover, the spa gene of EM-resistant cells was detected by polymerase chain reaction (PCR) and confirmed by quantitative real-time PCR assay, showing that the expression of SpA was repressed at the transcriptional level in these strains. Furthermore, ELISA assay showed that whole EM-resistant cell SpA content was significantly decreased. Therefore, it was considered that the suppression of surface SpA on the EM-resistant strain was due to regulated SpA production, and not dependent on the conformational change in SpA molecule expression through cell wall thickening. These results strongly suggest that suppressed SpA distribution on the EM-resistant S. aureus is a phenotypical characteristic in these strains.","PeriodicalId":18338,"journal":{"name":"Medical Molecular Morphology","volume":"30 1","pages":""},"PeriodicalIF":1.8,"publicationDate":"2024-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139928499","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transmission electron microscopic study of the surface layer of surgical resected disc specimens in human temporomandibular joint 人体颞下颌关节手术切除椎间盘标本表层的透射电子显微镜研究

IF 1.8 4区医学

Medical Molecular Morphology Pub Date : 2023-12-09 DOI: 10.1007/s00795-023-00376-7

Hiroaki Yoshida, Hiroki Ishikawa, Akio Himejima, Hayato Ikeda, Mitsuru Tani, Ryoji Taniguchi, T. Iseki, Yutaka Tsutsumi

引用次数: 0

Immunohistological evaluation of patients treated with intra-arterial chemoradiotherapy and surgery for oral cancer. 口腔癌动脉内放化疗及手术治疗患者的免疫组织学评价。

IF 1.8 4区医学

Medical Molecular Morphology Pub Date : 2023-12-01 Epub Date: 2023-07-29 DOI: 10.1007/s00795-023-00367-8

Yutaro Ikeuchi, Masanori Someya, Tomokazu Hasegawa, Masato Saito, Shoh Mafune, Takaaki Tsuchiya, Mio Kitagawa, Toshio Gocho, Hironari Dehari, Kazuhiro Ogi, Takanori Sasaki, Yoshihiko Hirohashi, Toshihiko Torigoe, Naoki Hirokawa, Akihiro Miyazaki, Koh-Ichi Sakata

{"title":"Immunohistological evaluation of patients treated with intra-arterial chemoradiotherapy and surgery for oral cancer.","authors":"Yutaro Ikeuchi, Masanori Someya, Tomokazu Hasegawa, Masato Saito, Shoh Mafune, Takaaki Tsuchiya, Mio Kitagawa, Toshio Gocho, Hironari Dehari, Kazuhiro Ogi, Takanori Sasaki, Yoshihiko Hirohashi, Toshihiko Torigoe, Naoki Hirokawa, Akihiro Miyazaki, Koh-Ichi Sakata","doi":"10.1007/s00795-023-00367-8","DOIUrl":"10.1007/s00795-023-00367-8","url":null,"abstract":"Preoperative intra-arterial chemoradiotherapy (IACRT) can improve the outcome and reduce the extent of surgery in patients with advanced oral cancer. However, the response to this regimen varies among patients, which may be related to the immune status of the tumor. We investigated the effects of proteins involved in tumor immunity on the outcomes of combined IACRT and surgery for oral cancer. We examined CD8 + and FoxP3 + tumor-infiltrating lymphocytes (TILs) and programmed death ligand 1 (PD-L1) expression on immune cells and tumor cells in pretreatment biopsy samples from 69 patients diagnosed with oral cancer treated with IACRT at our institution during 2000-2020. Patients with abundant CD8 + TILs had significantly better 5-year disease-specific survival (DSS) compared to that of patients with less infiltration of these cells (P = 0.016). Patients with higher FoxP3 + T-cells invasion had significantly better DSS compared to that of less FoxP3 (P = 0.005). Patients with high PD-L1 expression in tumor cells and immune cells had significantly better DSS than that of patients with low PD-L1 expression in these cells (P = 0.009 and P = 0.025, respectively). Collectively, these results suggest that the tumor immune microenvironment could affect outcomes of IACRT treatment in oral cancer.","PeriodicalId":18338,"journal":{"name":"Medical Molecular Morphology","volume":" ","pages":"288-296"},"PeriodicalIF":1.8,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9890935","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

PD-L2 overexpression on tumor-associated macrophages is one of the predictors for better prognosis in lung adenocarcinoma. 肿瘤相关巨噬细胞上PD-L2过表达是肺腺癌预后较好的预测因素之一。

IF 1.8 4区医学

Medical Molecular Morphology Pub Date : 2023-12-01 Epub Date: 2023-07-04 DOI: 10.1007/s00795-023-00361-0

Eri Matsubara, Yusuke Shinchi, Yoshihiro Komohara, Hiromu Yano, Cheng Pan, Yukio Fujiwara, Koei Ikeda, Makoto Suzuki

{"title":"PD-L2 overexpression on tumor-associated macrophages is one of the predictors for better prognosis in lung adenocarcinoma.","authors":"Eri Matsubara, Yusuke Shinchi, Yoshihiro Komohara, Hiromu Yano, Cheng Pan, Yukio Fujiwara, Koei Ikeda, Makoto Suzuki","doi":"10.1007/s00795-023-00361-0","DOIUrl":"10.1007/s00795-023-00361-0","url":null,"abstract":"Immunotherapies that target programmed cell death protein 1 (PD-1) signals are standard therapies for advanced-stage lung cancer, and the expression of programmed death-ligand 1 (PD-L1) in cancer tissue predicts immunotherapy efficacy. Although programmed death-ligand 2 (PD-L2) is expressed in cancer cells and macrophages, similar to PD-L1, its significance in lung cancer is unclear. Double immunohistochemistry analyses using anti-PD-L2 and anti-PU.1 antibodies were carried out on tissue array sections from 231 cases of lung adenocarcinoma, and PD-L2 expression in macrophages was evaluated. High PD-L2 expression in macrophages was associated with longer progression-free survival (PFS) and cancer-specific survival (CSS) and observed more often in females, non-heavy smokers, and patients with epidermal growth factor receptor (EGFR) mutations and those at a lower disease stage. Significant correlations were found more frequently in patients with EGFR mutations. Cell culture studies revealed that cancer cell-derived soluble factors induced PD-L2 overexpression in macrophages, suggesting the involvement of the JAK-STAT signaling pathway. The present findings suggest that PD-L2 expression in macrophages predicts PFS and CSS in lung adenocarcinoma without immunotherapy.","PeriodicalId":18338,"journal":{"name":"Medical Molecular Morphology","volume":" ","pages":"250-256"},"PeriodicalIF":1.8,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9803183","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expression analyses of WAC, a responsible gene for neurodevelopmental disorders, during mouse brain development. 小鼠脑发育过程中神经发育障碍相关基因WAC的表达分析。

IF 1.2 4区医学

Medical Molecular Morphology Pub Date : 2023-12-01 Epub Date: 2023-07-04 DOI: 10.1007/s00795-023-00364-x

Masashi Nishikawa, Tohru Matsuki, Nanako Hamada, Atsuo Nakayama, Hidenori Ito, Koh-Ichi Nagata

{"title":"Expression analyses of WAC, a responsible gene for neurodevelopmental disorders, during mouse brain development.","authors":"Masashi Nishikawa, Tohru Matsuki, Nanako Hamada, Atsuo Nakayama, Hidenori Ito, Koh-Ichi Nagata","doi":"10.1007/s00795-023-00364-x","DOIUrl":"10.1007/s00795-023-00364-x","url":null,"abstract":"WAC is an adaptor protein involved in gene transcription, protein ubiquitination, and autophagy. Accumulating evidence indicates that WAC gene abnormalities are responsible for neurodevelopmental disorders. In this study, we prepared anti-WAC antibody, and performed biochemical and morphological characterization focusing on mouse brain development. Western blotting analyses revealed that WAC is expressed in a developmental stage-dependent manner. In immunohistochemical analyses, while WAC was visualized mainly in the perinuclear region of cortical neurons at embryonic day 14, nuclear expression was detected in some cells. WAC then came to be enriched in the nucleus of cortical neurons after birth. When hippocampal sections were stained, nuclear localization of WAC was observed in Cornu ammonis 1 - 3 and dentate gyrus. In cerebellum, WAC was detected in the nucleus of Purkinje cells and granule cells, and possibly interneurons in the molecular layer. In primary cultured hippocampal neurons, WAC was distributed mainly in the nucleus throughout the developing process while it was also localized at perinuclear region at 3 and 7 days in vitro. Notably, WAC was visualized in Tau-1-positive axons and MAP2-positive dendrites in a time-dependent manner. Taken together, results obtained here suggest that WAC plays a crucial role during brain development.","PeriodicalId":18338,"journal":{"name":"Medical Molecular Morphology","volume":" ","pages":"266-273"},"PeriodicalIF":1.2,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9803184","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Aquaporins contribute to vacuoles formation in Nile grass type II diabetic rats. 水通道蛋白促进尼罗草II型糖尿病大鼠液泡形成。

IF 1.8 4区医学

Medical Molecular Morphology Pub Date : 2023-12-01 Epub Date: 2023-07-26 DOI: 10.1007/s00795-023-00365-w

Kana Aihara, Yosuke Nakazawa, Shun Takeda, Natsuko Hatsusaka, Takanori Onouchi, Noriko Hiramatsu, Mayumi Nagata, Noriaki Nagai, Megumi Funakoshi-Tago, Naoki Yamamoto, Hiroshi Sasaki

{"title":"Aquaporins contribute to vacuoles formation in Nile grass type II diabetic rats.","authors":"Kana Aihara, Yosuke Nakazawa, Shun Takeda, Natsuko Hatsusaka, Takanori Onouchi, Noriko Hiramatsu, Mayumi Nagata, Noriaki Nagai, Megumi Funakoshi-Tago, Naoki Yamamoto, Hiroshi Sasaki","doi":"10.1007/s00795-023-00365-w","DOIUrl":"10.1007/s00795-023-00365-w","url":null,"abstract":"Regulation of ion and water microcirculation within the lens is tightly controlled through aquaporin channels and connexin junctions. However, cataracts can occur when the lens becomes cloudy. Various factors can induce cataracts, including diabetes which is a well-known cause. The most common phenotype of diabetic cataracts is a cortical and/or posterior subcapsular opacity. In addition to the three main types and two subtypes of cataracts, a vacuole formation is frequently observed; however, their origin remains unclear. In this study, we focused on the aquaporins and connexins involved in diabetes-induced cataracts and vacuoles in Nile grass type II diabetes. The results showed that the expression of aquaporin 0 and aquaporin 5 increased, and that of connexin 43 decreased in diabetic rat lenses. Additionally, aquaporin 0 and 5 were strongly localized in peripheral of vacuoles, suggesting that aquaporins are involved in vacuoles formation. Transillumination photography revealed large vacuoles at the tip of the Y-suture in the anterior capsule of the diabetic lens, and several small vacuoles were observed in the posterior capsule. Within the vacuoles, cytoplasmic degradation and aggregation of fibrous material were observed. Our findings suggest that aquaporins are potential candidate proteins for preventing vacuole formation.","PeriodicalId":18338,"journal":{"name":"Medical Molecular Morphology","volume":" ","pages":"274-287"},"PeriodicalIF":1.8,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10229190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Micromorphological observation of HLE cells under knockdown of Fascin using LV-SEM. fassin敲除后HLE细胞的微观形态学观察。

IF 1.8 4区医学

Medical Molecular Morphology Pub Date : 2023-12-01 Epub Date: 2023-08-01 DOI: 10.1007/s00795-023-00362-z

Yoshihiro Hayashi, Yumiko Yamamoto, Ichiro Murakami

{"title":"Micromorphological observation of HLE cells under knockdown of Fascin using LV-SEM.","authors":"Yoshihiro Hayashi, Yumiko Yamamoto, Ichiro Murakami","doi":"10.1007/s00795-023-00362-z","DOIUrl":"10.1007/s00795-023-00362-z","url":null,"abstract":"Liver cancer is one of the most prevalent cancers in Japan with hepatocellular carcinoma (HCC) as the major histological subtype. Successful novel treatments for HCC have been reported; however, recurrences or metastasis may occur, which results in poor prognoses and high mortality of HCC patients. Fascin, an actin-bundling protein, regulates cell adhesion, migration, and invasion. Its overexpression positively correlates with poor prognosis of malignant tumors, and Fascin is considered as one of the tumor biomarkers and therapeutic target proteins. In this study, we attempted to reveal the relationship between Fascin and HCC using HLE, one of the human HCC cell lines. We performed the study with classical immunocytochemistry and recently developed techniques, such as wound-healing assay, spheroid cultivation, and low-vacuum scanning electron microscopy (LV-SEM). Non-Fascin-knockdown (FKD) cell spheroid had a regular spherical appearance with tight cell-cell connections, while FKD cell spheroid had an irregular shape with loose cell-cell connections. Cells of non-FKD spheroid presented fibrous protrusions on the cell surface, contrarily, cells of FKD spheroids showed bulbous-shaped protrusions. Morphological observation of FKD and non-FKD HLE spheroids were performed using LV-SEM. Our study may help to reveal the roles of Fascin in the process of HCC formation and its malignancy.","PeriodicalId":18338,"journal":{"name":"Medical Molecular Morphology","volume":" ","pages":"257-265"},"PeriodicalIF":1.8,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9912111","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A novel approach to diagnosing crystal-storing histiocytosis: utility of scanning electron microscopy for formalin-fixed paraffin-embedded tissue specimens. 一种诊断晶体储存组织细胞增多症的新方法:扫描电子显微镜对福尔马林固定石蜡包埋组织标本的应用。

IF 1.8 4区医学

Medical Molecular Morphology Pub Date : 2023-12-01 Epub Date: 2023-07-03 DOI: 10.1007/s00795-023-00363-y

Kazufumi Magara, Akira Takasawa, Keisuke Kikuchi, Taro Sugawara, Taro Murakami, Daisuke Kyuno, Yusuke Ono, Kumi Takasawa, Yasunao Numata, Shigeru Sasaki, Hiroshi Nakase, Tadashi Hasegawa, Makoto Osanai

{"title":"A novel approach to diagnosing crystal-storing histiocytosis: utility of scanning electron microscopy for formalin-fixed paraffin-embedded tissue specimens.","authors":"Kazufumi Magara, Akira Takasawa, Keisuke Kikuchi, Taro Sugawara, Taro Murakami, Daisuke Kyuno, Yusuke Ono, Kumi Takasawa, Yasunao Numata, Shigeru Sasaki, Hiroshi Nakase, Tadashi Hasegawa, Makoto Osanai","doi":"10.1007/s00795-023-00363-y","DOIUrl":"10.1007/s00795-023-00363-y","url":null,"abstract":"Crystal-storing histiocytosis (CSH) is a rare disorder that shows infiltration of histiocytes with an aberrant cytoplasmic accumulation of crystalline structures and is often accompanied by lymphoproliferative-plasma cell disorders (LP-PCD) as background diseases. The diagnosis of CSH requires identification of crystalline structures that accumulate in the infiltrating histiocytes, which may be challenging by optical microscopy alone. In this case report, we describe an atypical course of systemic CSH with multifocal fibrosclerosis of an unknown background disease that was diagnosed by ultrastructural observation, including transmission electron microscopy (TEM) and scanning electron microscopy (SEM), in pathological autopsy. In addition, crystalline structures were successfully identified by scanning electron microscopic observations using formalin-fixed and paraffin-embedded (FFPE) tissue from biopsy specimens taken before death. Since CSH was identified by SEM in a tiny biopsy specimen, observation of histiocytic infiltrative lesions by SEM using FFPE tissue may lead to early detection of and initiation of treatment for CSH.","PeriodicalId":18338,"journal":{"name":"Medical Molecular Morphology","volume":" ","pages":"297-302"},"PeriodicalIF":1.8,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9738149","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fast-track preparation of lung specimens for electron microscope observations of the pulmonary endothelial glycocalyx. 快速制备肺标本，用于电镜观察肺内皮糖萼。

IF 1.8 4区医学

Medical Molecular Morphology Pub Date : 2023-12-01 Epub Date: 2023-07-05 DOI: 10.1007/s00795-023-00360-1

Mone Wakatsuki, Takashi Takaki, Akira Ushiyama, Kazuho Honda, Takehiko Iijima

{"title":"Fast-track preparation of lung specimens for electron microscope observations of the pulmonary endothelial glycocalyx.","authors":"Mone Wakatsuki, Takashi Takaki, Akira Ushiyama, Kazuho Honda, Takehiko Iijima","doi":"10.1007/s00795-023-00360-1","DOIUrl":"10.1007/s00795-023-00360-1","url":null,"abstract":"The glycocalyx (GCX) covers the luminal surface of blood vessels and regulates vascular permeability. As GCX degradation predicts various types of vasculopathy, confirming the presence of this structure is useful for diagnosis. Since the GCX layer is very fragile, careful fixation is necessary to preserve its structure. We explored appropriate and feasible methodologies for visualizing the GCX layer using lung tissue specimens excised from anesthetized mice. Each specimen was degassed and immersed in Alcian blue (ALB) fixative solution, and then observed using electron microscopy. Specimens from septic mice were prepared as negative GCX controls. Using these immersion-fixed specimens, the GCX layer was successfully observed using both transmission and scanning electron microscopy; these observations were similar to those obtained using the conventional method of lanthanum perfusion fixation. Spherical aggregates of GCX were observed in the septic mouse specimens, and the GCX density was lower in the septic specimens than in the non-septic specimens. Of note, the presently reported methodology reduced the specimen preparation time from 6 to 2 days. We, therefore, concluded that our novel method could be applied to human lung specimens and could potentially contribute to the further elucidation of vasculopathies.","PeriodicalId":18338,"journal":{"name":"Medical Molecular Morphology","volume":" ","pages":"239-249"},"PeriodicalIF":1.8,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9808015","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction: A case of coexistent poorly differentiated adenosquamous carcinoma (glassy cell carcinoma), usual-type adenocarcinoma, and squamous cell carcinoma in situ of the cervix. 更正:1例宫颈低分化腺鳞癌(玻璃状细胞癌)、普通型腺癌和鳞状细胞原位癌共存。

IF 1.8 4区医学

Medical Molecular Morphology Pub Date : 2023-09-01 DOI: 10.1007/s00795-023-00358-9

Kouki Habara, Asami Nishikori, Jin Kiyama, Manami Nakashima, Masanori Koda, Kenji Sasaki, Tomohisa Sakashita, Norifumi Tanaka, Shuji Yonehara

引用次数: 0