International Journal of Medical Laboratory最新文献_第6页

The Relationship Between Salivary Candida Albicans Colony Count and Blood Group Antigens in Dentistry Students 牙科学生唾液白色念珠菌菌落计数与血型抗原的关系

International Journal of Medical Laboratory Pub Date : 2021-12-24 DOI: 10.18502/ijml.v8i4.8099

Samira Hajimaghsoodi, Abbasali Jafari Nodoushan, Mohamad Hassan Akhavan Karbassi, Yasaman Yazdanparast

{"title":"The Relationship Between Salivary Candida Albicans Colony Count and Blood Group Antigens in Dentistry Students","authors":"Samira Hajimaghsoodi, Abbasali Jafari Nodoushan, Mohamad Hassan Akhavan Karbassi, Yasaman Yazdanparast","doi":"10.18502/ijml.v8i4.8099","DOIUrl":"https://doi.org/10.18502/ijml.v8i4.8099","url":null,"abstract":"Background and Aims: Candida albicans is the most prevalent opportunistic fungal species in the oral cavity. To date, several studies have been investigated the various factors associated with oral candidiasis. On the other hand, it has been proven that blood types antigens lead to some infectious factors. This study aimed to evaluate Candida albicans colonies in the saliva of dentistry students based on their blood type to detect a relationship between blood group and incidence of oral candidiasis. \u0000Materials and Methods: In this descriptive cross-sectional study, 200 dentistry students were selected by a simple sampling method, including 100 individuals with blood type O and 100 with other blood types. The unstimulated salivary samples of all the participants were collected by spitting, cultured on Sabouraud medium, and then the isolated Candida albicans colonies were enumerated and recorded. \u0000Results: In the present study, samples comprised 77 males and 123 females, of whom 15.5% (31 individuals) carried colony-forming units > 40. The mean of Candida albicans colonies in the individuals' saliva with blood type O was 21.55, and it was 10.68 in the other groups. Besides, the differences were statistically significant (p = 0.024). There was no significant difference in Candida albicans colony count between O positive and O negative blood groups. \u0000Conclusions: The result of this study showed a significant relationship between the number of Candida albicans colonies of saliva and the individual’s blood type.","PeriodicalId":183358,"journal":{"name":"International Journal of Medical Laboratory","volume":"59 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126135997","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nonlinear Optical Investigation of Biochemical Analytes in Blood Serum via Z-Scan Technique 用z扫描技术对血清生化分析物的非线性光学研究

International Journal of Medical Laboratory Pub Date : 2021-12-24 DOI: 10.18502/ijml.v8i4.8100

Samane Raji, M. A. Haddad, S. M. Moshtaghioun, Z. Dehghan

{"title":"Nonlinear Optical Investigation of Biochemical Analytes in Blood Serum via Z-Scan Technique","authors":"Samane Raji, M. A. Haddad, S. M. Moshtaghioun, Z. Dehghan","doi":"10.18502/ijml.v8i4.8100","DOIUrl":"https://doi.org/10.18502/ijml.v8i4.8100","url":null,"abstract":"Background and Aims: Biomolecules' optical and nonlinear optical properties are widely used for different medical diagnoses and applications in biophotonic devices. These properties are essential in studying biological processes in living tissues. Aside from biomolecules' linear optical characteristics, their nonlinear optical characteristics have lately been considered. \u0000Materials and Methods: In this paper, the nonlinear optical responses of three biochemical analytes in blood serum, including glucose, triglycerides, and cholesterol are examined using the laser-based Z-Scan technique. Hence, different laboratory samples of blood serum with various concentrations of biochemical analytes are taken from patients for this purpose. \u0000Results: The normalized transmission curves were taken and analyzed in a closed aperture configuration of the Z-Scan technique and led to evaluating the nonlinear refractive index (n2) as an indicator of nonlinear responses of samples. The results show significant nonlinear optical behaviors of the samples under a strong electrical field of the laser, which leads to the measurement of the nonlinear refractive index (n2) of the samples with an error of less than ̴ 0.5 × 10-8 cm2/w. It is found that the values of nonlinear refractive index (n2) vary proportionally as a function of their concentration in blood serum. \u0000Conclusion: The findings of this study imply that the above sample's nonlinear optical response and, probable, those of other biological tissues might be used in medical diagnoses. In addition, the Z-Scan technique might be considered as a complementary method with other conventional diagnostic procedures due to its simplicity and quick experimental approach.","PeriodicalId":183358,"journal":{"name":"International Journal of Medical Laboratory","volume":"23 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132247555","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Sensitization to Food and Aeroallergens in Patients with Asthma, Allergic Rhinitis, Eczema and Urticaria 哮喘、变应性鼻炎、湿疹和荨麻疹患者对食物和空气过敏原的致敏

International Journal of Medical Laboratory Pub Date : 2021-12-24 DOI: 10.18502/ijml.v8i4.8097

Abbas Dabbaghzadeh, J. Ghaffari, J. Yazdani-charati, Marziyeh Mohammadi Kordkheyli, Fatemeh Pouresmaeil

{"title":"Sensitization to Food and Aeroallergens in Patients with Asthma, Allergic Rhinitis, Eczema and Urticaria","authors":"Abbas Dabbaghzadeh, J. Ghaffari, J. Yazdani-charati, Marziyeh Mohammadi Kordkheyli, Fatemeh Pouresmaeil","doi":"10.18502/ijml.v8i4.8097","DOIUrl":"https://doi.org/10.18502/ijml.v8i4.8097","url":null,"abstract":"Background and Aims: Allergic disorders such as asthma, urticaria, eczema, and allergic rhinitis are common worldwide, and allergens are the most common etiology and exacerbating factors. So, This study aimed to find the allergens in these patients with skin prick tests. \u0000Materials and Methods: All the allergic cases referred to an allergic clinic in the north of Iran were visited by an allergist and clinical immunologist. Based on patient history, physical examination, and diagnosis, we select food allergens and aeroallergens. A standard skin prick test was performed on all patients, and all data was then analyzed SPSS 20. \u0000Results: Two thousand one hundred and twenty-eight cases entered the study with a mean age of 27.65 ± 15.52 years old. 1235 (58.04%) females and 893 males (41.96%) participated in this study. Prevalence of allergic rhinitis, asthma, urticaria, and eczema were 717 (33.7%), 611 (28.8%), 550 (25.8%), and 250 (11.7%), respectively. The most common aeroallergens were Dermatophagoides farinae (75.9%), Dermatophagoides pteronyssinus (65.4%), feather (56.1%), and Candida (51.1%), respectively, in all patients. The most common food allergens were egg white (31.1%), cacao (29.7%), and egg yolk (28.9%), respectively, in all cases. \u0000Conclusion: Both aeroallergens and food allergens were the most common in all allergens by skin prick test. Therefore, it is important to find the relationship between sensitization and allergy.","PeriodicalId":183358,"journal":{"name":"International Journal of Medical Laboratory","volume":"18 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115152734","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

TNF-α, iNOS Augmentation Due to Macrophages and Neutrophils Activity in Samples from Patients in Intensive Care Unit with COVID-19 Infection 重症监护病房感染COVID-19患者标本中巨噬细胞和中性粒细胞活性导致TNF-α、iNOS升高

International Journal of Medical Laboratory Pub Date : 2021-12-22 DOI: 10.18502/ijml.v8i4.8092

Z. Zangeneh, A. Andalib, Gholamreza Khamisipour, Hamid Saadabadimotlagh, Sareh Zangeneh, N. Motamed

{"title":"TNF-α, iNOS Augmentation Due to Macrophages and Neutrophils Activity in Samples from Patients in Intensive Care Unit with COVID-19 Infection","authors":"Z. Zangeneh, A. Andalib, Gholamreza Khamisipour, Hamid Saadabadimotlagh, Sareh Zangeneh, N. Motamed","doi":"10.18502/ijml.v8i4.8092","DOIUrl":"https://doi.org/10.18502/ijml.v8i4.8092","url":null,"abstract":"Background and Aims: Cells and secreted molecules by the innate immune system are the essential factors in the pathogenesis and determining the severity of inflammation in COVID-19 patients. Severe inflammation results from increased activity of neutrophils, macrophages, and other cells with their products. Inflammatory cytokines such as tumor necrosis factor-a (TNF-a) increases the severity and pathogenesis of the disease caused by the virus. Phagocytes are armed with inducible nitric oxide synthase (iNOS), that upon stimulation by proinflammatory cytokines augment an immune response against pathogens. \u0000Materials and Methods: Two groups of patients were included with COVID-19 infection from the intensive care unit (ICU, n=52) and (non-ICU-care, n=54). Blood samples were collected to measure cells and serum parameters, including lymphocytes, neutrophils, platelet counts, accompanied with C-reactive protein, lactate dehydrogenase, TNF-a and iNOS levels. \u0000Results: In the ICU group, increased white blood cells (p=0.048), decreased lymphocytes (p=0.0007), increased neutrophils (p=0.001), decreased platelets, increase serum levels for lactate dehydrogenase (p =0.0001), c-reactive protein (p=0.003), TNF-a (p=0.018), and iNOS (p=0.008) were statistically obtained. Positive correlations were calculated between TNF-a and iNOS (r=0.65, p=0.0002) and with c-reactive protein (r=0.52, p=0.003) and with lactate dehydrogenase (r=0.68, p=0.0001). \u0000Conclusion: Inflammation due to macrophages and neutrophils activity in COVID-19 patients and increased mediators correlate with disease progression. It seems that control of the cell activity and their inflammatory cytokines would be considered for therapeutic goals. Changing the polarization of inflammatory macrophages to anti-inflammatory macrophages with therapeutic applications could prevent the severity of the provocative course of the disease.","PeriodicalId":183358,"journal":{"name":"International Journal of Medical Laboratory","volume":"8 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127836497","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

The Comparison of Susceptibility to SARS-CoV-2 Infection between Pediatric and Adults 儿童与成人对SARS-CoV-2感染易感性的比较

International Journal of Medical Laboratory Pub Date : 2021-12-22 DOI: 10.18502/ijml.v8i4.8083

Milad Zandi, S. Soltani, M. Fani, Haniye Shafipour, S. Abbasi

引用次数: 0

Clinical Presentations of Iranian Confirmed COVID-19 Patients at the Admission Time: A Preliminary Retrospective Study 伊朗新冠肺炎确诊患者入院时临床表现的初步回顾性研究

International Journal of Medical Laboratory Pub Date : 2021-12-22 DOI: 10.18502/ijml.v8i4.8095

Alireza Tabibzade, M. Abbasi, Sajad Karampoor, M. H. Karbalaie Niya, Maryam Esghaei, S. Mousavi, H. Zahednasab, Parastoo Yousefi, H. Keyvani

{"title":"Clinical Presentations of Iranian Confirmed COVID-19 Patients at the Admission Time: A Preliminary Retrospective Study","authors":"Alireza Tabibzade, M. Abbasi, Sajad Karampoor, M. H. Karbalaie Niya, Maryam Esghaei, S. Mousavi, H. Zahednasab, Parastoo Yousefi, H. Keyvani","doi":"10.18502/ijml.v8i4.8095","DOIUrl":"https://doi.org/10.18502/ijml.v8i4.8095","url":null,"abstract":"Background and Aims: The current preliminary study aimed to assess the clinical symptoms of the 67 Iranian COVID-19 patients and investigate the possible beneficial effects of the naproxen compared to the standard therapeutic regimen. \u0000Materials and Methods: We assessed 67 COVID-19 patients. All COVID-19 cases were confirmed by computed tomography (CT) and real time-polymerase chain reaction tests. We evaluated the clinical symptoms of the patients at the admission time. Also, a group of 28 patients received naproxen besides their standard treatment. Clinical presentations, radiographic features, white blood cells (WBC) in peripheral blood, hemoglobin, platelets, C-reactive protein, erythrocyte sedimentation rate, blood urea nitrogen, lactate dehydrogenase, Albumin, and Creatine Phosphokinase were evaluated. \u0000Results: The patients' clinical symptoms show that cough (89.6%) was the most repeated signed at the admission time, followed by fever at 78.7%, fatigue at 70%, and myalgia at least 64.2%. Unilateral slight ground-glass opacity was the most abundant presentation by 64.1% in CT. The laboratory assessment in patients indicates that mean WBC was 6193 ± 3258 (x106/L), and mean lymphocyte was 27.8 ± 12%. The survival rate and the hospitalization days for patients with or without the Naproxen regimen were not statistically significant. \u0000Conclusion: The most common clinical symptoms in Iranian patients with COVID-19 at the admission time include cough, fever, fatigue, and myalgia. Based on the current study results, the survival rate and the hospitalization days for patients with or without Naproxen usage were not statistically significant. The laboratory parameters could not show any particular statistically significant differences.","PeriodicalId":183358,"journal":{"name":"International Journal of Medical Laboratory","volume":"18 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134604478","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of Thymol on the Level of Bcl-2 Family Transcript in the Hypertrophied Heart of Rats 百里香酚对肥大大鼠心脏Bcl-2家族转录物水平的影响

International Journal of Medical Laboratory Pub Date : 2021-08-10 DOI: 10.18502/ijml.v8i3.7329

F. Safari, Reza Atarodi, H. Jamshidi

{"title":"Effect of Thymol on the Level of Bcl-2 Family Transcript in the Hypertrophied Heart of Rats","authors":"F. Safari, Reza Atarodi, H. Jamshidi","doi":"10.18502/ijml.v8i3.7329","DOIUrl":"https://doi.org/10.18502/ijml.v8i3.7329","url":null,"abstract":"Background and Aims: Long-term surge of heart loads causes cell hypertrophy. Left ventricular hypertrophy is an adaptive response of the heart to pathological stimuli such as hypertension. B-cell lymphoma 2 (Bcl-2) family members play an essential role in this process regulation. This study aimed to evaluate the effect of thymol on the transcription level of Bcl-2 family factors in the rat model of left ventricular hypertrophy. \u0000Materials and Methods: Male Wistar rats were divided into four groups: 1- Control 2-Untreated hypertrophy (H), 3 and 4 groups which received 25 and 50 mg/kg/day of thymol (H + Tym25 and H + Tym50 groups, respectively). Hypertrophy was induced by abdominal aortic banding, and the real time polymerase chain reaction technique was used for gene expression. \u0000Results: Data showed that in the H group, the mRNA level of the BAD was increased significantly (p ˂ 0.001). However, the transcription level of BAX was increased in the H and H+Tym25 compared with the control group. In the H + Tym50 group, BAX mRNA level decreased significantly compared to the H group (p ˂ 0.05). \u0000Conclusions: Our findings demonstrated that the expression rates of the antiapoptotic factor, Bcl-2, was significantly increased in the H group (p < 0.01) and thymol-treated hypertrophy groups (p < 0.001). Interestingly, the upregulation of Bcl-2 mRNA was statistically significant in the H+Tym50 group compared with H and H + Tym25 groups (p < 0.01). The results showed that thymol could protect heart hypertrophied by increasing the expression of anti-apoptotic factors.","PeriodicalId":183358,"journal":{"name":"International Journal of Medical Laboratory","volume":"69 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123376501","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Neuroinflammatory State of Multiple Sclerosis and Strategies for Biotherapeutics Development 多发性硬化症的神经炎症状态和生物治疗发展策略

International Journal of Medical Laboratory Pub Date : 2021-08-10 DOI: 10.18502/ijml.v8i3.7323

S. Soltanmoradi, F. Kouhkan, Iman Rad

{"title":"Neuroinflammatory State of Multiple Sclerosis and Strategies for Biotherapeutics Development","authors":"S. Soltanmoradi, F. Kouhkan, Iman Rad","doi":"10.18502/ijml.v8i3.7323","DOIUrl":"https://doi.org/10.18502/ijml.v8i3.7323","url":null,"abstract":"Multiple Sclerosis (MS) is the most prevalent neurological disability in young adults. The pathogenesis of MS is characterized by demyelination and neurodegeneration in the central nervous system (CNS) as the ruinous result of chronic activation of the immune system. All clinical forms of MS, including relapsing-remitting multiple sclerosis (RRMS), secondary progressive multiple sclerosis (SPMS), and the primary progressive MS (PPMS), demonstrate inflammation as a common symptom. In various autoimmune diseases like MS, the ability of the immune system to set a balance between pro-inflammatory and anti-inflammatory responses is lost. In this review, the imbalance between pro-inflammatory and anti-inflammatory responses of immune cells and their role in MS progression is discussed. Disturbing the balance of Th1/Th2 and Th17/Treg cells and M1/M2 phenotypes of macrophages and microglial plays a key role in the development and progression of MS. In this review, we first depict an outline of regulatory immune cells involved in inflammation. Second, we discuss shreds of evidence that confirm how B cells play both pathogenic and protective roles in MS disease. Third, we point out the pros and cons of B cell/T cell-targeted therapies in clinical trials.","PeriodicalId":183358,"journal":{"name":"International Journal of Medical Laboratory","volume":"19 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133862733","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Cutaneous Leishmaniasis in Suspected Patients Referred to the Health Centers of Abarkouh, Ardakan, Bafgh and Khatam, Yazd Province, Iran 疑似患者皮肤利什曼病转诊至伊朗亚兹德省阿巴尔库赫、阿达坎、巴夫赫和哈塔姆卫生中心

International Journal of Medical Laboratory Pub Date : 2021-08-10 DOI: 10.18502/ijml.v8i3.7332

S. Ahmadi, G. Eslami, M. Vakili, K. Barzegar., Ali - Fattahi Bafghi

{"title":"Cutaneous Leishmaniasis in Suspected Patients Referred to the Health Centers of Abarkouh, Ardakan, Bafgh and Khatam, Yazd Province, Iran","authors":"S. Ahmadi, G. Eslami, M. Vakili, K. Barzegar., Ali - Fattahi Bafghi","doi":"10.18502/ijml.v8i3.7332","DOIUrl":"https://doi.org/10.18502/ijml.v8i3.7332","url":null,"abstract":"Background and Aims: Cutaneous Leishmaniasis is a significant health problem in many parts of Iran. Management of the disease and its treatment is a global dilemma. In this study, the status and the proportions of Cutaneous Leishmaniasis induced by Leishmania major and tropica among suspected patients referred to the Health Centers of Abarkouh, Ardakan, Bafgh, and Khatam cities, Yazd Province, Iran were investigated. \u0000Materials and Methods: The lesion was diagnosed using direct smear microscopy and conventional polymerase chain reaction. \u0000Results: A total of 90 samples were prepared of which 64 (71.1%) were male and 26 (27.9%) were female. Also, 30 (33.3%) samples came from Ardakan, 29 (32.2%) samples from Bafgh, 21 (23.3%) samples from Abarkouh, and 10 (11.1%) samples from Khatam city. Ninety samples with 112 lesions were recruited and parasitologically examined. The results showed that, in macroscopic examination, 90 of the patients had 59 lesions: patients with lesion(s) induced by Leishmania major = 52 (M=35, 67.3%, F=17, 32.7%), lesion(s) induced by Leishmania tropica =4 (M=2, 50.0%, F=2, 50.0%), and lesion(s) induced by false positive =33 (36.4%). Basides, in microscopic method, 90 of the patients had 59 lesions: patients with lesion(s) induced by Leishmania major =44 (M=29, 65.9%, F=15, 34.09%), lesion(s) induced by Leishmania tropica =9 (M=8, 88.8%, F=1, 11.2%), and lesion(s) induced by false positive =37 (M=28, 75.7%, F=9, 24.3%). \u0000Conclusion: Although travel history to an endemic area is important for diagnosis, parasitological confirmation is necessary to initiate treatment.","PeriodicalId":183358,"journal":{"name":"International Journal of Medical Laboratory","volume":"5 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127531884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Detection of blaVIM and blaIMP Carbapenemase-Producing Pseudomonas aeruginosa from Clinical Samples in Iran 伊朗临床样品中blaim和blaIMP产碳青霉烯酶假单胞菌的检测

International Journal of Medical Laboratory Pub Date : 2021-08-10 DOI: 10.18502/ijml.v8i3.7324

M. Zolfaghari

{"title":"Detection of blaVIM and blaIMP Carbapenemase-Producing Pseudomonas aeruginosa from Clinical Samples in Iran","authors":"M. Zolfaghari","doi":"10.18502/ijml.v8i3.7324","DOIUrl":"https://doi.org/10.18502/ijml.v8i3.7324","url":null,"abstract":"Background and Aims: The prevalence of carbapenemase-producing Pseudomonas aeruginosa (P. aeruginosa) strains has been recently reported worldwide. Therefore, accurate and rapid detection of carbapenemase-producing isolates is essential. So, this study aimed to detect blaVIM and blaIMP carbapenemase-producing strains using the modified Hodge test (MHT) and reverse transcription-polymerase chain reaction (RT-PCR). \u0000Materials and Methods: In this cross-sectional study, P. aeruginosa strains were collected from clinical samples (blood, urine, wound, and other liquids body) in Firoozgar and Shahid Motahari Hospitals in Tehran and Velayat Hospital in Rasht Province, from May to December 2018. After identifying the isolates using the standard microbial tests, carbapenemase-producing strains were isolated by the modified hodge test. After that, the detection of blaVIM and blaIMP genes was performed by RT-PCR technique. \u0000Results: One hundred P. aeruginosa were isolated from different clinical samples. Among these, 74 (74%) isolates were considered as carbapenemase positive using MHT. The frequencies of blaVIM and blaIMP genes were obtained as 83% and 11%, respectively. \u0000Conclusions: The results of this study indicate a high level of resistance to most of the antibiotics tested and a high prevalence of blaVIM gene in P. aeruginosa strains.","PeriodicalId":183358,"journal":{"name":"International Journal of Medical Laboratory","volume":"9 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134580398","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0