International Journal of Medical Laboratory最新文献_第5页

In Vitro Study of Hyaluronic Acid Based Scaffolds and Its Effect on Cartilage Regeneration 透明质酸支架的体外研究及其对软骨再生的影响

International Journal of Medical Laboratory Pub Date : 2022-04-03 DOI: 10.18502/ijml.v9i1.9081

A. Rajabi, M. Pourentezari, Hengameh Dortaj, A. Shahedi, M. Yadegari, Sepideh Izadi, Fatemeh Zakizadeh, Zeinolabedin Sharifian Dastjerdi

{"title":"In Vitro Study of Hyaluronic Acid Based Scaffolds and Its Effect on Cartilage Regeneration","authors":"A. Rajabi, M. Pourentezari, Hengameh Dortaj, A. Shahedi, M. Yadegari, Sepideh Izadi, Fatemeh Zakizadeh, Zeinolabedin Sharifian Dastjerdi","doi":"10.18502/ijml.v9i1.9081","DOIUrl":"https://doi.org/10.18502/ijml.v9i1.9081","url":null,"abstract":"Recently, it has been proven that cartilage healing is difficult. The most commonly used treatments are autogenously cartilage grafting and allogeneic bone grafting, but grafts cannot fully meet treatment goals because of source, price, safety, and other concerns. Thus, a combination of biological materials and tissue engineering technology has become a recent trend in studies. Among the studies performed on tissue engineering cartilage materials are hydrogels that exhibit biological activity, post-decomposition adsorption, flexibility, and easy preparation. Cell-containing hydrogels are often used in cartilage tissue engineering because of their biocompatibility, ease of use, and ability to adapt to different defects. Hydrogels are used to mimic extracellular matrices. Although multiple materials can configure and form hydrogels, hyaluronic acid and its derivatives are distinguished. Hyaluronic acid (HA) is an extracellular molecule with several physical and biological functions found in many tissues, including cartilage. HA is formed in several biomaterial systems and scaffolding. HA hydrogels have many interests, including increased adhesion, cell proliferation, and wound healing. In addition, they represent adequate biological acting for stimulating a microenvironment for the survival of cells. However, their disadvantages include a slow degradation rate and low mechanical properties. Here, HA-based hydrogels and their applications in cartilage tissue engineering are briefly reviewed.","PeriodicalId":183358,"journal":{"name":"International Journal of Medical Laboratory","volume":"82 2 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123812894","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Study on Bacterial Agents of Patients with Meningitis Referred to Afzalipour Hospital in Kerman, South East Iran 伊朗东南部克尔曼市Afzalipour医院转诊脑膜炎患者细菌性病原体的研究

International Journal of Medical Laboratory Pub Date : 2022-04-03 DOI: 10.18502/ijml.v9i1.9080

M. Farokhnia, A. Hosseininasab, Mohadeseh Kamali, B. Pourabbas, M. Taheri Sarvtin

引用次数: 0

CNV Analysis Using Multiplex Ligation-Dependent Probe Amplification in Iranian Families with Non-Syndromic Congenital Heart Defects: Early Diagnosis of Non-Syndromic Patients 使用多重结扎依赖探针扩增在伊朗非综合征性先天性心脏缺陷家庭中进行CNV分析:非综合征性患者的早期诊断

International Journal of Medical Laboratory Pub Date : 2022-04-03 DOI: 10.18502/ijml.v9i1.9087

Soheila Khaksari, Ehsan Aghaei Moghadam, Ahoura Nozari, Zahra Boroughani, S. Ghasemi Firouzabadi, F. Behjati

{"title":"CNV Analysis Using Multiplex Ligation-Dependent Probe Amplification in Iranian Families with Non-Syndromic Congenital Heart Defects: Early Diagnosis of Non-Syndromic Patients","authors":"Soheila Khaksari, Ehsan Aghaei Moghadam, Ahoura Nozari, Zahra Boroughani, S. Ghasemi Firouzabadi, F. Behjati","doi":"10.18502/ijml.v9i1.9087","DOIUrl":"https://doi.org/10.18502/ijml.v9i1.9087","url":null,"abstract":"Background and Aims: Congenital heart defects (CHD) are the most common type of congenital disability. Copy number variations (CNVs) have been found as one of the genetic etiology of non-syndromic CHD, and researchers have detected several pathogenic CNVs in patients with cardiac defects. \u0000Materials and Methods: In the present study, 70 patients with familial (20 patients) and sporadic (50 patients) non-syndromic CHD were evaluated to find whether CNVs in the GATA4, NKX2-5, TBX-5, CREL, BMP4 genes, and 22q11.2 region contribute to the pathogenesis of non-syndromic CHD. We have used the Multiplex Ligation-dependent Probe Amplification (MLPA) technique as a molecular method to identify CNVs in predefined loci. \u0000Results: Normal MLPA results were demonstrated for GATA4, NKX2-5, TBX-5, CRELD, and BMP4 genes for all sporadic and familial cases. However, we found three patients with imbalances for the 22q11.2 region. One patient with 22q11.2 deletion showed tetralogy of fallot, and the other had ventricular septal defects/ pulmonary atresia/ multiple aortopulmonary collateral arteries. A duplication of the 22q11.2 region was detected in one patient with patent ductus arteriosus. \u0000Conclusion: Identifying genomic imbalances in 6% of the non-syndromic sporadic patients indicates that recurrent CNVs could be associated with non-syndromic CHD. It seems that it is the first CNV analysis using MLPA carried out in Iranian patients with cardiac defects. We suggest that 22q11.2 imbalances should be considered in patients with cardiac lesions to provide an accurate diagnosis and appropriate genetic counseling in affected families.","PeriodicalId":183358,"journal":{"name":"International Journal of Medical Laboratory","volume":"5 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124215618","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Type of Bacterial Isolates and Antibiotic Resistance Patterns from Clinical Specimens in Yazd, Iran 伊朗亚兹德临床标本细菌分离类型和抗生素耐药模式

International Journal of Medical Laboratory Pub Date : 2022-04-03 DOI: 10.18502/ijml.v9i1.9083

A. Torki, M. Zahedi, N. Amirmozafari

{"title":"Type of Bacterial Isolates and Antibiotic Resistance Patterns from Clinical Specimens in Yazd, Iran","authors":"A. Torki, M. Zahedi, N. Amirmozafari","doi":"10.18502/ijml.v9i1.9083","DOIUrl":"https://doi.org/10.18502/ijml.v9i1.9083","url":null,"abstract":"Background and Aims: Due to a wide variety of causes, various bacteria can acquire resistance to antibiotics. This study investigated the antibiotic susceptibility patterns of the etiologic bacterial agents of various infections in patients referred to three hospitals in Yazd city, Iran. \u0000Materials and Methods: A total of 336 clinical specimens including wound discharge, sputum, blood, bronchial fluid, pleural fluid, ascitic fluid, synovial fluid, stool, and trachea secretions were collected in three hospitals. Microbiological culturing in order to grow and identify the causative bacteria were performed. Antibiotic susceptibility determinations were done by the Kirby Bauer disk diffusion method. \u0000Result: Among all organisms isolated, Escherchia coli, Staphylococcus. aureus, Staphylococcus. suprophyticus, Acinetobacter spp., Pseudomonas spp., Klebsiella spp., Staphylococcus. epidermidis and Entrococcus spp. were the most prevalent, respectively. The Escherchia coli isolates were the most antibiotic-resistant Gram-negative bacterium. It seems that the same organisms recovered from different hospital wards show different antibiotic susceptibility patterns. \u0000Conclusion: The increased prevalence of resistant organisms in different hospitals may reflect a lack of proper antibiotic usage policy, resulting in the prolonged and indiscriminate use of antimicrobial agents.","PeriodicalId":183358,"journal":{"name":"International Journal of Medical Laboratory","volume":"8 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126408194","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Isolation and Characterization of Lytic Bacteriophages from Wastewater against Listeria monocytogenes 废水中抗单核增生李斯特菌裂解噬菌体的分离与鉴定

International Journal of Medical Laboratory Pub Date : 2022-04-03 DOI: 10.18502/ijml.v9i1.9082

A. Marzban, Marjan Beiranvand, Samaneh Sedighi-Khavidak

{"title":"Isolation and Characterization of Lytic Bacteriophages from Wastewater against Listeria monocytogenes","authors":"A. Marzban, Marjan Beiranvand, Samaneh Sedighi-Khavidak","doi":"10.18502/ijml.v9i1.9082","DOIUrl":"https://doi.org/10.18502/ijml.v9i1.9082","url":null,"abstract":"Background and Aims: In the current study, two bacteriophages were isolated from the wastewater sources and characterized by lytic activity against the Listeria monocytogenes (L. monocytogenes) bacterium. \u0000Materials and Methods: 50 wastewater samples were collected from the disposal sites. The phages were isolated from the treated samples using the double agar overlay method with L. monocytogenes as the host bacterium. Plaque morphology, temperature, pH, titration of phage lysate, host range, and scanning electron microscopic morphology were used to characterize the isolated phages. \u0000Results: Bacteriophage was found in two samples taken from wastewater treatment plants. Phages were proliferated and released under optimal bacterial growth conditions at pH 7; 37 °C in 36-hour incubation. A 10:1 ratio of bacterial cells to phage produced the greatest lytic ability. The highest phage titrated under optimal conditions was estimated at 9×109 pfu/ml. The scanning electron microscopic image revealed a bacteriophage morphology corresponding to the head and tail group. The host range observations demonstrated that L. monocytogenes were unique to the isolated phage. The elimination of L. monocytogenes growth in milk and whey samples revealed that this bacteriophage targeted bacteria and resulted in a 75% reduction in bacterial contamination. \u0000Conclusion: The results suggest that the phages obtained from L. monocytogenes may be utilized to combat this pathogen in dairy, agri-food, wastes, and other associated infections.","PeriodicalId":183358,"journal":{"name":"International Journal of Medical Laboratory","volume":"56 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127705623","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Root Cause Analysis of Haemolysis Encountered in Leuco-filtration of Stored Packed Red Cells Units 储存红细胞白细胞滤过中溶血的根本原因分析

International Journal of Medical Laboratory Pub Date : 2022-04-03 DOI: 10.18502/ijml.v9i1.9086

Amita R, Arya AB PGDBBT, Sathyabhama S, Debasish Gupta

引用次数: 0

The Effect of Sitagliptin on Inflammatory Mediators in the Ovary of Rat with Polycystic Ovary Syndrome 西格列汀对多囊卵巢综合征大鼠卵巢炎症介质的影响

International Journal of Medical Laboratory Pub Date : 2022-04-03 DOI: 10.18502/ijml.v9i1.9085

A. Safaeian, M. Izadi, F. Zare Mehrjerdi, M. Yadegari, Mohammad Ebrahim Rezvani

{"title":"The Effect of Sitagliptin on Inflammatory Mediators in the Ovary of Rat with Polycystic Ovary Syndrome","authors":"A. Safaeian, M. Izadi, F. Zare Mehrjerdi, M. Yadegari, Mohammad Ebrahim Rezvani","doi":"10.18502/ijml.v9i1.9085","DOIUrl":"https://doi.org/10.18502/ijml.v9i1.9085","url":null,"abstract":"Background and Aims: Polycystic ovary syndrome (PCOS) is an endocrine and metabolic disease in females of reproductive age and is a significant infertility cause. Inflammation plays a crucial role in the pathogenesis of PCOS. The present study evaluated whether sitagliptin, dipeptidyl peptidase-4 inhibitor, attenuates inflammatory markers C-reactive protein (CRP), interleukin(IL)-6, tumor necrosis factor-α (TNF-α), IL-1β, and transforming growth factor-β (TGF-β) in a rat model of PCOS. Materials and Methods: Twenty-two female adult Wistar rats were randomly divided into four groups: control, PCOS model, PCOS+sitagliptin (25 mg/kg), and PCOS+sitagliptin (50 mg/kg). PCOS was induced by injection of estradiol valerate, intraperitoneally. Sitagliptin was gavaged daily for 30 days to both groups of animals. After the treatment period, blood and ovaries tissue were collected to analyze inflammatory parameters. Results: The mRNA levels of IL-6, IL-1β, TGF-β, and TNF-α in the PCOS model group were markedly elevated compared with the control group (p<0.01). These parameters' mRNA levels were reduced in the sitagliptin treatment groups compared with the PCOS group (p<0.01). Also, the serum concentration of CRP in the PCOS group was more than the control. This increase significantly decreased in groups treated with sitagliptin compared with the PCOS group. Conclusion: The presented study suggested that the protective effects of sitagliptin on PCOS may be due to its inhibitory effect on expression and inflammatory markers' levels.","PeriodicalId":183358,"journal":{"name":"International Journal of Medical Laboratory","volume":"91 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115175159","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Molecular and Clinical Characterization of 7 Iranian Patients with Severe Congenital Factor V Deficiency: Identification of 4 Novel Mutations 7例伊朗严重先天性因子V缺乏患者的分子和临床特征:4个新突变的鉴定

International Journal of Medical Laboratory Pub Date : 2022-04-03 DOI: 10.18502/ijml.v9i1.9084

Sedighe Satari, Mahmood Shams, S. Tabibian, F. Zaker, M. Rezvany

{"title":"Molecular and Clinical Characterization of 7 Iranian Patients with Severe Congenital Factor V Deficiency: Identification of 4 Novel Mutations","authors":"Sedighe Satari, Mahmood Shams, S. Tabibian, F. Zaker, M. Rezvany","doi":"10.18502/ijml.v9i1.9084","DOIUrl":"https://doi.org/10.18502/ijml.v9i1.9084","url":null,"abstract":"Background and Aims: Congenital factor V (FV) deficiency is a rare bleeding disorder with 1 in 1000000 persons in the general population. Individuals with FV activity <1% and very low FV antigen levels are characterized as severe FV deficient patients. Little data is available about the molecular basis of this bleeding disorder in Iran. \u0000Materials and Methods: We analyzed 7 unrelated Iranian FV deficient patients regarding clinical manifestation and genotype. The molecular dynamic simulation was carried out to analyze the effect of novel mutations on the FV structure. \u0000Results: All cases had recurrence epistaxis, oral cavity bleeding, and hematoma were frequent in our patients. The molecular analysis led to the identification of three already reported mutations (IVS 19+3 A>T, 4014-4017 del TCAG and p.P419R) and four novel mutations (IVS9-1 G>C, Y478D, L1844P, I1556T) in the FV gene of our patients. According to the molecular modeling results, it seems that in the two mutations Y478D and I1556T, an increased number of H-bonds in mutant proteins compared to natural ones reduces the flexibility and increases the stability of the mutant proteins. The results also show that in L1844P and I1556T mutations, the total solvent accessible surface area (both hydrophilic and hydrophobic) significantly decreases compared to the natural variants. \u0000Conclusion: Identifying the causative mutation in patients with FV deficiency helps to determine the molecular basis of this bleeding disorder and gain more insight into explaining the variable clinical manifestations of patients with FV deficiency.","PeriodicalId":183358,"journal":{"name":"International Journal of Medical Laboratory","volume":"40 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121522590","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Motivation and Intention to Future Donation among Blood Donors Attending a Tertiary Care Hospital Blood Bank in Kerala 喀拉拉邦三级医院血库献血者的献血动机和未来献血意向

International Journal of Medical Laboratory Pub Date : 2021-12-24 DOI: 10.18502/ijml.v8i4.8101

Vijayalakshmi Kuttath, Shyamily Mohan

引用次数: 2

The Effect of Autophagy Induction in Oncolytic Reovirus Replication in Mesenchymal Stem Cells 自噬诱导对间充质干细胞溶瘤性呼肠孤病毒复制的影响

International Journal of Medical Laboratory Pub Date : 2021-12-24 DOI: 10.18502/ijml.v8i4.8098

S. Maleki, H. Soleimanjahi, A. Abdoli, H. Karimi, R. S. Banijamali, T. Bamdad

{"title":"The Effect of Autophagy Induction in Oncolytic Reovirus Replication in Mesenchymal Stem Cells","authors":"S. Maleki, H. Soleimanjahi, A. Abdoli, H. Karimi, R. S. Banijamali, T. Bamdad","doi":"10.18502/ijml.v8i4.8098","DOIUrl":"https://doi.org/10.18502/ijml.v8i4.8098","url":null,"abstract":"Background and Aims: Oncolytic reoviruses can infect and kill malignant cells while sparing their normal counterparts. Reoviral infection can induce or activate autophagy, even though metformin can induce autophagy. Identifying and regulating the cellular pathways important for reovirus replication and oncolysis can improve targeted-biological therapies for cancer. Here, the autophagic process was triggered via metformin, and we investigated the effect of autophagy activation on oncolytic reovirus replication in mesenchymal stem cells as primary cells and L929 cell lines. \u0000Materials and Methods: Adipose derived mesenchymal stem cells (AD-MSCs) and L929 cells were treated with metformin and reovirus type-3 strain Dearing (T3D). Twenty-four hours after infection, the viability of AD-MSCs and L929 cells were examined by MTT assay. Also, the effect of metformin-induced autophagy in the reovirus replication in these cells was determined by real-time polymerase-chain-reaction. \u0000Results: Our results show that treatment with metformin and reovirus reduced the viability of the cells compared to treatment with metformin or reovirus alone in both cells. Also, coadministration of metformin and reovirus significantly decreased the relative expression level of the Beclin-1 gene compared to treatment with metformin in both cells. However, the expression level of the reovirus L3 gene after treatment with metformin and reovirus in L929 cells increased significantly compared to AD-MSCs. \u0000Conclusion: Our data suggest that metformin-induced autophagy enhances reoviral replication in AD-MSCs and L929 cells. These findings represent the role of autophagy induction in facilitating reovirus replication and contribute to a better understanding of reovirus-host interactions.","PeriodicalId":183358,"journal":{"name":"International Journal of Medical Laboratory","volume":"11 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130651773","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0