International Journal of Medical Laboratory最新文献

The Importance of Antioxidant/Anti-inflammatory Vitamins during the SARS-CoV-2 Infection 抗氧化/抗炎维生素在SARS-CoV-2感染中的重要性

International Journal of Medical Laboratory Pub Date : 2023-06-20 DOI: 10.18502/ijml.v10i2.12945

M. Nabi-Afjadi, H. Karami, K. Goudarzi, Zahra Abasian, Nafiseh Heidari-Kalvani, Fereshte Barjasteh, E. Bahreini

引用次数: 0

Investigating the Prevalence of Glucose-6-Phosphate Dehydrogenase (G6PD) Deficiency among Patients with Favism Symptoms in Kerman City, Southern Iran 调查伊朗南部克尔曼市蚕豆病症状患者中葡萄糖-6-磷酸脱氢酶(G6PD)缺乏症的患病率

International Journal of Medical Laboratory Pub Date : 2023-06-20 DOI: 10.18502/ijml.v10i2.12950

Mohadeseh Kamali, M. Taheri Sarvtin

{"title":"Investigating the Prevalence of Glucose-6-Phosphate Dehydrogenase (G6PD) Deficiency among Patients with Favism Symptoms in Kerman City, Southern Iran","authors":"Mohadeseh Kamali, M. Taheri Sarvtin","doi":"10.18502/ijml.v10i2.12950","DOIUrl":"https://doi.org/10.18502/ijml.v10i2.12950","url":null,"abstract":"Background and Aims: Glucose-6-phosphate dehydrogenase (G6PD) deficiency is humans’ most common erythrocyte enzyme defect. About 400 million people are estimated to be affected by this disorder worldwide. Antimalarial drugs, especially primaquine, and other oxidative stress, can cause hemolytic complications in G6PD deficient individuals. This study aimed to evaluate the prevalence of G6PD deficiency in Kerman City in southern Iran. \u0000Materials and Methods: This descriptive cross-sectional study was conducted from 2016 to 2021. Blood samples were taken from all patients with symptoms of G6PD deficiency who were referred to a general hospital in Kerman City in southern Iran. The G6PD enzyme activity was measured qualitatively by fluorescent spot test. \u0000Results: A total of 6369 patients were included in this study. G6PD deficiency was seen in 424 (6.7%) subjects. Of 424 patients, 359 (84.7%) were severely G6PD deficient, and 65 (15.3%) patients exhibited partial deficiency. G6PD deficiency was seen in 324 (9.3%) males and 100 (3.4%) females (p < 0.001). \u0000Conclusion: The results of our study confirmed the existence of G6PD deficiency in a significant percentage of patients in Kerman City. Therefore, many people in this city are exposed to hemolytic complications if they use antimalarial drugs and other oxidative substances. According to our results, testing G6PD deficiency and monitoring the potential primaquine toxicity in patients who receive primaquine are highly recommended.","PeriodicalId":183358,"journal":{"name":"International Journal of Medical Laboratory","volume":"9 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127991539","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bone Marrow Karyotype, Flow Cytometry, and FISH Analysis: Essential Tests to Improve the Initial Diagnosis of Patients with Myeloid Malignancies 骨髓核型、流式细胞术和FISH分析:提高髓系恶性肿瘤患者初始诊断的基本试验

International Journal of Medical Laboratory Pub Date : 2023-06-20 DOI: 10.18502/ijml.v10i2.12947

F. Naseri, Fatemeh Safari, H. Khoshnevis, Maryam Pilechian Langroudi, S. Hesami, Nazanin Taheri, Abbas Shakoori Farahani

{"title":"Bone Marrow Karyotype, Flow Cytometry, and FISH Analysis: Essential Tests to Improve the Initial Diagnosis of Patients with Myeloid Malignancies","authors":"F. Naseri, Fatemeh Safari, H. Khoshnevis, Maryam Pilechian Langroudi, S. Hesami, Nazanin Taheri, Abbas Shakoori Farahani","doi":"10.18502/ijml.v10i2.12947","DOIUrl":"https://doi.org/10.18502/ijml.v10i2.12947","url":null,"abstract":"Background and Aims: Diagnosing hematologic malignancies requires implementing several tests. This study aims to evaluate the chromosomal changes in patients with myeloid disorders and compare the results of flow cytometry and cytogenetics with the initial diagnosis performed by the oncologist. \u0000Materials and Methods: 115 patients with myeloid disorders, 57.2% males and 42.8% females with a mean age of 50.3 years, previously diagnosed by an oncologist based on the clinical features, complete blood count, and peripheral blood smear interpretations, were considered. Moreover, flow cytometry and cytogenetic analysis were implemented on the bone marrow samples. \u0000Results: Cytogenetic results showed that 30% of patients with myeloid disorders had abnormal karyotypes. 77% of patients with myelodysplastic syndromes, 65% of acute myeloid leukemia, and 30.7% of chronic myeloid leukaemia indices showed normal karyotypes, and the others resulted in common and uncommon abnormalities, including the translocation (13;17), 92, XXYY, and del (4q). Considering the flow cytometry and karyotype results, the improved diagnoses were made for 41 patients who had not been diagnosed initially. \u0000Conclusion: This study showed that, in some cases, an initial diagnosis is inconsistent with the flow cytometry and karyotype analysis results. Also, the flow cytometry results may differ from the karyotype depending on the case. Therefore, combining the results obtained by the cytogenetic investigation, flow cytometry, fluorescence in situ hybridization, and molecular testing is preferable to provide a comprehensive report for the appropriate disease diagnosis and prognosis.","PeriodicalId":183358,"journal":{"name":"International Journal of Medical Laboratory","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116559751","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Study on Prevalence, Morphology and Morphometric of Gongylonema pulchrum in Sheep Slaughtered in Sari, Northern Iran 伊朗北部萨里地区屠宰绵羊中弓形线虫的流行、形态和形态计量学研究

International Journal of Medical Laboratory Pub Date : 2023-06-20 DOI: 10.18502/ijml.v10i2.12949

Tahereh Mikaeili Galeh, M. Nakhaei, A. Daryani, S. Sarvi, S. Hosseini, S. Gholami

{"title":"A Study on Prevalence, Morphology and Morphometric of Gongylonema pulchrum in Sheep Slaughtered in Sari, Northern Iran","authors":"Tahereh Mikaeili Galeh, M. Nakhaei, A. Daryani, S. Sarvi, S. Hosseini, S. Gholami","doi":"10.18502/ijml.v10i2.12949","DOIUrl":"https://doi.org/10.18502/ijml.v10i2.12949","url":null,"abstract":"Background and Aims: The genus Gongylonema infects the upper digestive tract of birds, mammals, and humans. Livestock parasites, especially in the gastrointestinal tract, have caused irreparable damage, including death, reduced products, and economic losses. The present study aimed to reveal the prevalence and morphological and morphometric study of Gongylonema spp. isolated from the esophagus of slaughtered sheep in Sari, northern Iran. \u0000Materials and Methods: Esophagi of 340 sheep were collected from Sari's industrial slaughterhouse during the summer and autumn of 2021 (summer=115, autumn= 225). After longitudinal cutting, the surfaces of the esophageal mucosa were carefully checked under the light. The prevalence of infection was estimated, and the morphometric and morphological characteristics of the isolated worms were investigated using a calibrated light microscope and camera lucida. \u0000Results: The prevalence of infection was 7.6% (26 isolates), which was estimated for summer and autumn 12 out of 115 (10.4%) and 14 out of 225 (6.2%), respectively. A comparison of morphological features of nematodes in this study with Gongylonema morphological criteria showed that all of them were Gongylonema pulchrum. \u0000Conclusions: In the present study, a moderate gongylonemiasis infection was estimated in the sheep from Sari's industrial slaughterhouse. Morphological examination of the retrieved worms showed characteristic morphologies of Gongylonema pulchrum. To clarify the status of the infection, several morphological and molecular studies should be performed on different types of intermediate and final hosts of the parasite in different parts of the country.","PeriodicalId":183358,"journal":{"name":"International Journal of Medical Laboratory","volume":"13 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127505250","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Insights into Candida Albicans: A New Perspective on Pathogenic Factors and Regulatory Mechanisms 白色念珠菌:致病因素和调控机制的新视角

International Journal of Medical Laboratory Pub Date : 2023-06-20 DOI: 10.18502/ijml.v10i2.12943

Mohadeseh Kamali, M. T. Sarvtin

{"title":"Insights into Candida Albicans: A New Perspective on Pathogenic Factors and Regulatory Mechanisms","authors":"Mohadeseh Kamali, M. T. Sarvtin","doi":"10.18502/ijml.v10i2.12943","DOIUrl":"https://doi.org/10.18502/ijml.v10i2.12943","url":null,"abstract":"Candida albicans (C. albicans) is a polymorphic fungus that exists as a natural flora in the skin and mucosal surfaces of the body. However, under certain conditions, such as immunodeficiency, mucosal damage, antibiotic use, and cancer, this fungus can cause superficial and systemic infections. C. albicans is the most common opportunistic pathogenic fungus in humans and causes 60% of mucosal infections and 40% of candidemia cases. Several pathogenic factors have been identified that contribute to the pathogenic potential of this fungus. Among these factors, we can mention: hypha production, attachment, and biofilm formation, secretion of hydrolase enzymes, acquisition of micronutrients, adaptation to oxygen and nitrogen deficiency conditions, and growth at temperatures above 37 °C. This review article will investigate the pathogenic factors of C. albicans and their regulatory factors. For this purpose, articles published in national and international scientific databases, including PubMed/MEDLINE, Google Scholar, Elsevier databases, IranMedex, Scopus, SID, and Science Direct, were used. Keywords such as: “Candida,” “Fungi,” “Pathogenesis,” and “Virulence” were used to find the articles.","PeriodicalId":183358,"journal":{"name":"International Journal of Medical Laboratory","volume":"24 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124862849","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of the Phytocompound Combination against Dysbiosis Induced by AGE-Rich High-fat Diet in Mice 植物复合制剂对高脂饮食所致小鼠生态失调的影响

International Journal of Medical Laboratory Pub Date : 2023-06-20 DOI: 10.18502/ijml.v10i2.12948

F. Ghanbari, Navid Abedpoor, M. Peymani, S. Asadi-Yousefabad, Marzieh Lotfi, M. Sheikhha, Mahta Mazaheri-Naeini

{"title":"Effects of the Phytocompound Combination against Dysbiosis Induced by AGE-Rich High-fat Diet in Mice","authors":"F. Ghanbari, Navid Abedpoor, M. Peymani, S. Asadi-Yousefabad, Marzieh Lotfi, M. Sheikhha, Mahta Mazaheri-Naeini","doi":"10.18502/ijml.v10i2.12948","DOIUrl":"https://doi.org/10.18502/ijml.v10i2.12948","url":null,"abstract":"Background and Aims: The composition of the Microbiota can be influenced by various lifestyle and environmental factors, including diet. We designed a study to investigate the improving effects of plant extract, the combination of turmeric, ginger, boswellia, and cat’s claw on the abundance of key members of the gut microbiota, Bacteroidetes, Akkermansia muciniphila (A. muciniphila), Faecalibacterium prausnitzii (F. prausnitzii), Firmicutes and Bifidobacterium in mice treated with advanced glycation end products (AGE) rich high-fat diet (HFD). \u0000Materials and Methods: Eighteen 2-month age male C57BL/6 mice were adopted to a regular diet for 1 week and then fed with an HFD or regular diet. After 8 weeks of diet, animals received plant extract concurrently with HFD for 8 weeks. Stools were taken, DNA of stool samples was extracted, and qPCR of 16s rDNA universal primers was performed. Then the effect of plant extract on dysbiosis induced by AGE-rich HFD was accessed. \u0000Results: Our results revealed the frequencies of Bacteroidetes (p=0.001), A. muciniphila (p= 0.0005), F. prausnitzii (p<0.0001), and Bifidobacterium (p= 0.0008) were reduced, whereas the frequency of Firmicute was increased in the AGE-rich HFD group. A significant increase in the F/B ratio was observed in the HFD group than in the regular diet group (p= 0.0003). plant extract reduced the F/B ratio and improved gut microbiota homeostasis. \u0000Conclusion: Plant extract restored gut microbiota patterns in HFD-treated mice. It seems more studies are required to prove the application of plant extracts for modulating the gut microbiota as a promising new biomarker with potential for therapeutic applications.","PeriodicalId":183358,"journal":{"name":"International Journal of Medical Laboratory","volume":"317 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134483065","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Identification of Diagnostic SNPs in Exon 28 of the Von Willebrand Factor Gene for Diagnosis of VWD Type 2B in Patients Referred to IBTO Coagulation Lab: In-silico Approach 用于IBTO凝血实验室诊断VWD 2B型患者的Von Willebrand因子基因28外显子诊断snp的鉴定:计算机方法

International Journal of Medical Laboratory Pub Date : 2023-06-20 DOI: 10.18502/ijml.v10i2.12946

Roshanak Shamriz, K. Atarodi, M. Ahmadinejad, N. Ghasemi

{"title":"Identification of Diagnostic SNPs in Exon 28 of the Von Willebrand Factor Gene for Diagnosis of VWD Type 2B in Patients Referred to IBTO Coagulation Lab: In-silico Approach","authors":"Roshanak Shamriz, K. Atarodi, M. Ahmadinejad, N. Ghasemi","doi":"10.18502/ijml.v10i2.12946","DOIUrl":"https://doi.org/10.18502/ijml.v10i2.12946","url":null,"abstract":"Background and Aims: von Willebrand disease (VWD) is brought on by hereditary impairments in the von Willebrand factor (VWF). It has been determined that most of the molecular abnormalities are present in exon 28 of the VWF gene qualitative variations. In this regard, investigating mutations in exon 28 of the VWF coding gene can help identify the VWD type and can be used to manage patients by using appropriate strategies. \u0000Materials and Methods: 10 suspected VWD type 2B patients were investigated for the genotype of exon 28. Routine coagulation tests were performed for the patients. Molecular sequencing was also used for the evaluation of mutations in exon 28. Determining the tertiary structure of VWF can aid in understanding its functional residues and interactions. The nsSNVs’ pathogenicity was examined utilizing potent bioinformatics methods. Step-by-step testing of the high-risk mutations was done using SIFT, PolyPhen-2, I-Mutant 2.0, PHD-SNPg, and SNPs&GO. Then, the secondary structure, amino acid conservation, and feature of amino acids were investigated via Protparam, Cofactor, Interprosurf, ConSurf, NetSurfP-2.0, and HOPE. \u0000Results: N1231T, V1229G, V1316M, and P1266Q missense mutations in VWF were detected. 3D structure of VWF predicted and evaluated. Putative conserved domains were identified. P1266Q and V1316M amino acid substitutions are predicted as “Not tolerated substitution” damaging and Disease, while V1229G and N1231T amino acid substitutions are predicted as “tolerated substitution” benign and Neutral. \u0000Conclusion: V1316M and P1266Q amino acid substitutions were determined as high-risk mutations using powerful bioinformatics tools in VWD patients.","PeriodicalId":183358,"journal":{"name":"International Journal of Medical Laboratory","volume":"282 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123310402","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ferroptosis in Autoimmune Disorders, Viral, and Bacterial Infections 自身免疫性疾病、病毒和细菌感染中的铁下垂

International Journal of Medical Laboratory Pub Date : 2023-06-20 DOI: 10.18502/ijml.v10i2.12944

A. Zafarani, Ali Amini

引用次数: 0

A Mouse Monoclonal Antibody Against Human IFN-γ and its Characters 小鼠抗人IFN-γ单克隆抗体及其性质研究

International Journal of Medical Laboratory Pub Date : 2023-04-09 DOI: 10.18502/ijml.v10i1.12427

{"title":"A Mouse Monoclonal Antibody Against Human IFN-γ and its Characters","authors":"","doi":"10.18502/ijml.v10i1.12427","DOIUrl":"https://doi.org/10.18502/ijml.v10i1.12427","url":null,"abstract":"Background and Aims: A monoclonal antibody (mAb) can unambiguously identify, quantify, and purify an antigen or particular epitope at a large scale. The superiority of these antibodies lies in their specificity for the antigenic determinant. So, this study aims to prepare mouse mAb-secreting hybridoma against human gamma interferon (IFN-γ) and determine the produced antibody's characters. \u0000Materials and Methods: Mouse splenic B lymphocytes immunized with recombinant human IFN-γ were fused with mouse SP2/0 cells. The hybridized cells were selected by hypoxanthine-aminopterin-thymidine and hypoxanthine-thymidine media to obtain monoclonal antibody-producing hybridoma cells. Finally, indirect enzyme-linked immunosorbent assay (ELISA), sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), and western blot were used to confirm the creation of antibody-secreting hybridoma cells. \u0000Results: mAb against IFN-γ were produced by fusing SP2/0 mouse non-secretory myeloma cell line with the spleen cells of immunized mice. This antibody's indirect ELISA optical density was 2.055 on average, and the desired antibody bands were confirmed in SDS-PAGE compared to Septicol® (commercial antibody). Also, in the western blot, the desired antibody could bind to the antigen. IFN-γ transferred on nitrocellulose membrane. In ELISA and western blot tests, anti-mouse IgG conjugated antibodies were used; therefore, the mAb IgG isotype was taken into consideration. \u0000Conclusion: In this study, a mouse mAb was obtained by immunization of Balb/C mice and fusion of spleen cells of these mice with the SP2/0 cells, which can specifically bind to recombinant human IFN-γ and can be used to detect IFN-γ secretion in all types of intracellular infections, including latent tuberculosis.","PeriodicalId":183358,"journal":{"name":"International Journal of Medical Laboratory","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"120986445","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characteristics of Diminutive Colorectal Polyps in Patients Undergoing Colonoscopy in an Educational, Therapeutic Hospital in Western Iran 伊朗西部一家教育治疗医院接受结肠镜检查患者的小结肠息肉特征

International Journal of Medical Laboratory Pub Date : 2023-04-09 DOI: 10.18502/ijml.v10i1.12420

H. Bashiri, Mahsa Madani, Lotfollah Asgari, A. Bozorgomid

{"title":"Characteristics of Diminutive Colorectal Polyps in Patients Undergoing Colonoscopy in an Educational, Therapeutic Hospital in Western Iran","authors":"H. Bashiri, Mahsa Madani, Lotfollah Asgari, A. Bozorgomid","doi":"10.18502/ijml.v10i1.12420","DOIUrl":"https://doi.org/10.18502/ijml.v10i1.12420","url":null,"abstract":"Background and Aims: Limited information is available on the frequency of advanced adenomas in diminutive colon polyps. Thus, this study aimed to investigate the pathological characteristics and the frequency of high-grade dysplasia in diminutive colorectal polyps in individuals referred to colonoscopy examination in Kermanshah, Iran. \u0000Materials and Methods: Demographics characteristics, location and diameter of the polyp, histological assessment of the polyps, grade, and others were retrieved from colonoscopy reports. \u0000Results and Conclusions: During the study period, 250 diminutive colorectal polyps were detected. The histological assessment showed that 36.4% were adenomatous, 32.8% were hyperplastic, and 30.8% were inflammatory polyps. Only two diminutive polyps (0.8%) had high-grade dysplasia, and the frequency of adenocarcinoma in our study was 0.4%. Besides, the frequency of adenomatous polyps was higher in the proximal versus the distal colon. These findings emphasize the urgent need for a colorectal cancer screening plan in the Iranian population to improve therapeutic outcomes.","PeriodicalId":183358,"journal":{"name":"International Journal of Medical Laboratory","volume":"25 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133605597","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0