International Journal of Medical Laboratory最新文献_第2页

Effects of Ubiquinone on Oxidant and Antioxidant Status in Hepatocellular Carcinoma Cell Line 泛醌对肝癌细胞氧化和抗氧化状态的影响

International Journal of Medical Laboratory Pub Date : 2023-04-09 DOI: 10.18502/ijml.v10i1.12428

Nafiseh Heidari-Kalvani, Sudabeh Fallah, Fereshte Barjasteh, E. Bahreini

{"title":"Effects of Ubiquinone on Oxidant and Antioxidant Status in Hepatocellular Carcinoma Cell Line","authors":"Nafiseh Heidari-Kalvani, Sudabeh Fallah, Fereshte Barjasteh, E. Bahreini","doi":"10.18502/ijml.v10i1.12428","DOIUrl":"https://doi.org/10.18502/ijml.v10i1.12428","url":null,"abstract":"Background and Aims: The concomitant use of antioxidants during chemotherapy is controversial. It is unknown whether antioxidants increase or decrease the effectiveness of anticancer drugs. Therefore, the present study aimed to investigate ubiquinone's cytotoxic and antioxidant effects on the HepG2 cell line. \u0000Materials and Methods: The HepG2 cell line was chosen as an experimental model for hepatocellular carcinoma in this study. The cytotoxic effect of ubiquinone was assessed as a function of time and concentration using the colorimetric MTT assay. The half-maximal inhibitory concentration (IC50) was determined to assess the cytotoxic effects of different ubiquinone concentrations. The protective impacts of ubiquinone on HepG2 cells were evaluated by assessing the oxidative stress profile. \u0000Results: The MTT showed that the IC50 after treatment with ubiquinone was 350 and 335 μM at 24 and 48 hours, respectively. Evaluation of redox homeostasis in HepG2 cells using three doses of ubiquinone, including IC50 and one dose higher and one dose lower than IC50, showed a decrease in oxidative stress and an increase in the antioxidant capacity of HepG2 cells in a dose-dependent manner (p < 0.01). An increase in redox hemostasis decreased the viability of HepG2 cells. \u0000Conclusion: Our results showed ubiquinone could reduce cancer cell survival by interfering in redox oxidative-redox status. Therefore, ubiquinone can be used as an antioxidant supplement along with chemotherapy drugs.","PeriodicalId":183358,"journal":{"name":"International Journal of Medical Laboratory","volume":"136 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131545215","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Hematological and Biochemical Parameters of β-Thalassemia Major Patients in Bushehr City: A Comparative Analysis 布什尔市β-地中海贫血重症患者血液学和生化指标的比较分析

International Journal of Medical Laboratory Pub Date : 2023-04-09 DOI: 10.18502/ijml.v10i1.12426

{"title":"Hematological and Biochemical Parameters of β-Thalassemia Major Patients in Bushehr City: A Comparative Analysis","authors":"","doi":"10.18502/ijml.v10i1.12426","DOIUrl":"https://doi.org/10.18502/ijml.v10i1.12426","url":null,"abstract":"Background and Aims: β-thalassemia is the most common genetic disorder worldwide. β-thalassemia major results in severe anemia and serious complications. So, this study aims to evaluate the hematological and biochemical markers in β-thalassemia major patients in Bushehr city. \u0000Materials and Methods: Our study included 94 transfusion-dependent β-thalassemia major were compared with 94 normal healthy subjects as controls. Hematological assessments included complete blood count indices. The biochemical evaluations included liver, kidney, and thyroid function tests, lipid profile, sodium, potassium, calcium, phosphorus, fasting blood sugar, and serum ferritin. \u0000Results: All hematological parameters in patients, such as hemoglobin (p < 0.01), hematocrit (p < 0.01), mean corpuscular volume (p < 0.05), and mean corpuscular hemoglobin (p <0.05), were significantly reduced compared to the control group except mean corpuscular hemoglobin concentration which was insignificant (p > 0.05). Higher levels of triglyceride, phosphorus, fasting blood sugar, aspartate aminotransferase, alanine transaminase, alkaline phosphatase, uric acid, total and direct bilirubin, and lower levels of cholesterol, high-density lipoprotein, and low-density lipoprotein were observed in patients in comparison to the control group (p < 0.05). Serum sodium, potassium, calcium, and albumin was not significantly different from the control group (p > 0.05). The prevalence of primary hypothyroidism (thyroid stimulating hormone > 4.5 mIU/l and T4 < 5.6 μg/dl) was reported at 5.31%. \u0000Conclusions: This study emphasized the necessity for regular follow-up and evaluation of β-thalassemia, which could be used to improve treatment protocols.","PeriodicalId":183358,"journal":{"name":"International Journal of Medical Laboratory","volume":"118 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133427196","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

MiR-1 Variations in Colorectal Cancer: Possible Implementation as a Potential Accessory Biomarker MiR-1在结直肠癌中的变异:可能作为潜在的辅助生物标志物

International Journal of Medical Laboratory Pub Date : 2023-04-09 DOI: 10.18502/ijml.v10i1.12423

Elahe Pirzadeh, S. H. Aghaee-Bakhtiari, N. Yaghoubi, Ali Mahmoudi, L. Jarahi, Abbas Abdollahi, S. Hashemy, Seyed Mahdi Hasanian, Farnaz Zahedi Avval

{"title":"MiR-1 Variations in Colorectal Cancer: Possible Implementation as a Potential Accessory Biomarker","authors":"Elahe Pirzadeh, S. H. Aghaee-Bakhtiari, N. Yaghoubi, Ali Mahmoudi, L. Jarahi, Abbas Abdollahi, S. Hashemy, Seyed Mahdi Hasanian, Farnaz Zahedi Avval","doi":"10.18502/ijml.v10i1.12423","DOIUrl":"https://doi.org/10.18502/ijml.v10i1.12423","url":null,"abstract":"Background and Aims: Colorectal cancer (CRC) is one of the most common human cancers. Currently, carcinoembryonic antigen (CEA) is used as the main standard biomarker of CRC, though this biomarker is not specifically made for CRC and, in a minority of cases, shows inadequate sensitivity. Therefore, searching for novel accessory biomarkers may fill these gaps in clinical management. miRNAs physiologically regulate various metabolic processes and are misregulated in various cancers. Therefore, the present investigation was conducted to evaluate miR-1 levels in CRC samples. \u0000Materials and Methods: The CRC and adjacent tissue samples were obtained from 24 patients. In addition, sera were collected from the patient group and 24 healthy controls. Total RNA was extracted from tissue samples, and cDNA was synthesized. Real-time PCR determined the expression of miR-1. Serum levels of CEA were also measured using a Monobind ELISA assay kit. \u0000Results: The level of miR-1 in CRC tumors was significantly down-regulated. Moreover, patients with metastasis showed lower expression of miR-1 compared to cases without metastasis; however, this difference was not statistically significant. The ROC curve for miR-1 showed an AUC of 0.69. In addition, ROC analysis revealed a sensitivity of 70.27% and a specificity of 62.96% for miR-1. \u0000Conclusion: There is still a need for new upcoming markers in addition to the main CRC biomarker, CEA. The levels of miR-1 in colorectal cancer tissue samples may provide additional information for the management and follow-up of CRC patients; though, the clinical application needs further studies.","PeriodicalId":183358,"journal":{"name":"International Journal of Medical Laboratory","volume":"48 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115080292","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Neutrophil Extracellular Traps in Inflammatory and Autoimmune Diseases and Cancer 炎症、自身免疫性疾病和癌症中的中性粒细胞胞外陷阱

International Journal of Medical Laboratory Pub Date : 2023-04-09 DOI: 10.18502/ijml.v10i1.12421

Kaveh Tari, Ayat Abbasi-Shahkouh, Seyedeh Fatemeh Azghadi, Kimiya Rashidan, Z. Mousavi, Mobarakeh Ajam-Hosseini, Reyhaneh Abriyan, A. Atashi

{"title":"Neutrophil Extracellular Traps in Inflammatory and Autoimmune Diseases and Cancer","authors":"Kaveh Tari, Ayat Abbasi-Shahkouh, Seyedeh Fatemeh Azghadi, Kimiya Rashidan, Z. Mousavi, Mobarakeh Ajam-Hosseini, Reyhaneh Abriyan, A. Atashi","doi":"10.18502/ijml.v10i1.12421","DOIUrl":"https://doi.org/10.18502/ijml.v10i1.12421","url":null,"abstract":"Neutrophils are innate immune system phagocytes that play a central role in immunity defense. They are equipped with effective antimicrobial that is mainly stored in specialized granules. Considering that, it can also damage host tissue. Neutrophil deployment is heavily regulated through various strategies, including phagocytosis, reactive oxygen species, production degranulation, and the formation of neutrophil extracellular traps (NET). This review article will discuss its role in inflammatory, autoimmune diseases, and cancer and place it as a therapeutic target. It depicts that NET formation includes a suicide program morphologically different from other types of cell death, such as apoptosis and necrosis. Besides, NETs have unique DNA and antimicrobial peptide structures, and antimicrobial activity is among the functions of neutrophils as the first response to inflammation. So, it plays a pivotal role in the pathophysiology of various diseases, especially inflammatory and autoimmune diseases such as systemic lupus erythematosus and rheumatoid arthritis. As a result, it can be effective in the pathogenesis of many diseases, and its pathogenic role can be used as a therapeutic target.","PeriodicalId":183358,"journal":{"name":"International Journal of Medical Laboratory","volume":"21 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131455682","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of DNA Damage and Repair In In Vitro Expanded Cord Blood CD 34 Positive Hematopoietic Stem Cell 体外扩增脐带血cd34阳性造血干细胞DNA损伤与修复的评价

International Journal of Medical Laboratory Pub Date : 2023-04-09 DOI: 10.18502/ijml.v10i1.12424

Mohammad Shokouhian, H. Mozdarani, M. Soleimani, M. Safa, M. Rezvany

{"title":"Evaluation of DNA Damage and Repair In In Vitro Expanded Cord Blood CD 34 Positive Hematopoietic Stem Cell","authors":"Mohammad Shokouhian, H. Mozdarani, M. Soleimani, M. Safa, M. Rezvany","doi":"10.18502/ijml.v10i1.12424","DOIUrl":"https://doi.org/10.18502/ijml.v10i1.12424","url":null,"abstract":"Background and Aims: The occurrence of single and double-strand breaks of DNA damage is the major obstacle for proliferation under various environmental factors and, if not repaired, can result in many consequences, including mutation, cell death, and others. So, the present study was conducted to evaluate the damage of DNA and the expression status of DNA repair system genes before and after stem cell proliferation. \u0000Materials and Methods: The MACS method isolated the umbilical cord blood hematopoietic stem cells (UCB-HSCs). In order to investigate cell death, the study of Annexin V/PI was done by flow cytometry. Comet assay made observation and identification of DNA breaks, and the expression of genes normally involved in the repair of DNA breaks was evaluated by real-time polymerase chain reaction. \u0000Results: The average number of stem cells increased by 1.9-fold after three days of proliferation. The apoptotic percentage of cells was negligible (less than 0.2%), and the purity of the CD34+ cells was reduced by about one-third in three days (67%). By examining the expression of DNA repair genes, including KU70, KU80, RAD51, and XRCC1, their increased fold change was not significant. In a microscopic examination of stem cells in the comet assay, there was no significant difference between DNA damage before (1.33% ± 0.31) and after (2.08% ± 0.92) replication. \u0000Conclusion: In our investigation, neither DNA damage nor changes in the DNA break repair were observed. However, further studies are required to clarify the DNA break repair by recruiting more UCB-HSCs samples.","PeriodicalId":183358,"journal":{"name":"International Journal of Medical Laboratory","volume":"78 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127416831","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Inhibitory Effects of Lactobacillus Agilis Against Pseudomonas Aeruginosa Biofilm Formation and Evaluation of PpyR (PA2663) and algD Gene Expression 敏捷乳杆菌对铜绿假单胞菌生物膜形成的抑制作用及PpyR (PA2663)和algD基因表达的评价

International Journal of Medical Laboratory Pub Date : 2023-04-09 DOI: 10.18502/ijml.v10i1.12422

Yasaman Issazadeh, Mohadeseh Farnaghizad, S. Falsafi, H. Mazaheri, Shokoofeh Ghazi, Ava Behrouzi

{"title":"The Inhibitory Effects of Lactobacillus Agilis Against Pseudomonas Aeruginosa Biofilm Formation and Evaluation of PpyR (PA2663) and algD Gene Expression","authors":"Yasaman Issazadeh, Mohadeseh Farnaghizad, S. Falsafi, H. Mazaheri, Shokoofeh Ghazi, Ava Behrouzi","doi":"10.18502/ijml.v10i1.12422","DOIUrl":"https://doi.org/10.18502/ijml.v10i1.12422","url":null,"abstract":"Background and Aims: Hospital infections and their antibiotic resistance have become a global concern recently. One of the most prominent factors in hospital infections is Pseudomonas aeruginosa (P. aeruginosa), which can become resistant to many antibiotics due to its ability to form biofilms. Recently, scientists have tried to replace antibiotic therapy with alternative therapies such as probiotics which can reduce or eliminate the pathogenic bacteria's ability to form biofilms. Therefore, the present study revealed that some genes, such as algD and PpyR, were involved in biofilm formation in P. aeruginosa. Furthermore, the inhibitory effect of the supernatant of lactobacillus agilis on the biofilm formation of P. aeruginosa was evaluated in the current study. \u0000Materials and Methods: In this study, the effect of the supernatant of probiotic Lactobacillus agilis on the biofilm formation of P. aeruginosa and also the expression of two genes effective in biofilm formation (algD and PpyR) were investigated. Antibiograms were performed to detect the most resistant bacteria since there is a link between biofilm formation and antibiotic resistance. Further, the effects of probiotics on the expression of PpyR and algD genes were discussed. \u0000Results: Results showed that the biofilm formation of P. aeruginosa was significantly reduced in the presence of lactobacillus agilis. \u0000Conclusions: According to the current study, it could be concluded that because of antibiotics resistance and their associated mechanisms, probiotics could be used as a replacement for antibiotics in many treatments.","PeriodicalId":183358,"journal":{"name":"International Journal of Medical Laboratory","volume":"19 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124014026","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification and Quantification of Waddlia Chondrophila in Women with Recurrent Pregnancy Loss 复发性流产妇女Waddlia软骨病的鉴定和定量

International Journal of Medical Laboratory Pub Date : 2023-01-03 DOI: 10.18502/ijml.v9i4.11622

Saeed Harirzadeh, F. Kafilzadeh, H. Zandi, M. Kargar

{"title":"Identification and Quantification of Waddlia Chondrophila in Women with Recurrent Pregnancy Loss","authors":"Saeed Harirzadeh, F. Kafilzadeh, H. Zandi, M. Kargar","doi":"10.18502/ijml.v9i4.11622","DOIUrl":"https://doi.org/10.18502/ijml.v9i4.11622","url":null,"abstract":"Background and Aims: Recurrent pregnancy loss (RPL) is one of the important complications of pregnancy. Only 50% of cases have a known cause of RPL. One of the causes of RPL is Waddlia Chondrophila (W. Chondrophila), but no information is available regarding its in Iran. This study aimed to develop a Taqman Real-Time polymerase chain reaction assay to detect recA Gene of W. Chondrophilain biological samples from women with RPL in Yazd city. \u0000Materials and Methods: Clinical samples of women with a history of RPL, normal childbirth, and standard strain WSU 86–1044 were provided. Primers and probes of W. Chondrophila were designed. Positive control was provided based on the PUC57 vector. Technical performance was examined using a control plasmid. \u0000Results: Analytical sensitivity was 5 ng/µl of control plasmid DNA. No cross-amplification was observed when testing other pathogens that can detect in human vaginitis. The Taqman Real-Time PCR assay exhibited good reproducibility. This Real-Time PCR was then applied to 100 vaginal swabs. No samples were revealed to be Waddlia positive. \u0000Conclusions: This new TaqMan Real-Time PCR assay represents a diagnostic tool that may be used to further study the prevalence of W. Chondrophila infection in clinical specimens.","PeriodicalId":183358,"journal":{"name":"International Journal of Medical Laboratory","volume":"485 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133732391","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Study on the Prevalence and Susceptibility Pattern of MRSA in a Tertiary Care Hospital of Haroti Region 哈罗蒂地区某三级医院耐甲氧西林金黄色葡萄球菌流行及易感模式的研究

International Journal of Medical Laboratory Pub Date : 2023-01-03 DOI: 10.18502/ijml.v9i4.11621

A. Garg, L. Agarwal, Mamta Gupta, M. Agarwal

{"title":"A Study on the Prevalence and Susceptibility Pattern of MRSA in a Tertiary Care Hospital of Haroti Region","authors":"A. Garg, L. Agarwal, Mamta Gupta, M. Agarwal","doi":"10.18502/ijml.v9i4.11621","DOIUrl":"https://doi.org/10.18502/ijml.v9i4.11621","url":null,"abstract":"Background and Aims: Emerging antimicrobial resistance is one of the major public health threats worldwide. It can result in increased morbidity and mortality rates along with increased treatment costs and hospitalization stays. Methicillin-resistant Staphylococcus aureus (MRSA) has become a global challenge. It is a major cause of nosocomial and community-acquired infections. Its prevalence varies with country and within hospitals within a country. The current study estimates the prevalence of MRSA. \u0000Materials and Methods: A total of 162 Staphylococcus aureus strains were isolated from various clinical specimens, and antibiotic susceptibility tests and identification by cefoxitin disc (30 μg) were performed as per Clinical and Laboratory Standards Institute guidelines. \u0000Results: Among 162 Staphylococcus aureus 92 isolates were found to be MRSA. The highest rate of resistance was detected for penicillin (100), followed by erythromycin (97%), clindamycin (57%), tetracycline (13%), sulfamethoxazole-trimethoprim (SXT) (55%), ciprofloxacin (23%), and gentamicin (2%). All of the isolates were susceptible to linezolid and vancomycin. \u0000Conclusions: Treatment of multi-drug resistant MRSA is still problematic because of the limited choice of antibiotic. This analysis will assist clinicians to choose an appropriate course of action for MRSA infections.","PeriodicalId":183358,"journal":{"name":"International Journal of Medical Laboratory","volume":"38 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130445037","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Compound Heterozygous HPD Mutation in an Iranian Patient with Hypertyrosinemia Type III 伊朗III型高酪氨酸血症患者的复合杂合HPD突变

International Journal of Medical Laboratory Pub Date : 2023-01-03 DOI: 10.18502/ijml.v9i4.11619

F. Sarkargar, Seyed Ali Madani Manshadi, Ehsan Zare Mehrjardi, Hosein Khodaei, S. Kalantar, Seyed Ahmad Mohamamdi

{"title":"A Compound Heterozygous HPD Mutation in an Iranian Patient with Hypertyrosinemia Type III","authors":"F. Sarkargar, Seyed Ali Madani Manshadi, Ehsan Zare Mehrjardi, Hosein Khodaei, S. Kalantar, Seyed Ahmad Mohamamdi","doi":"10.18502/ijml.v9i4.11619","DOIUrl":"https://doi.org/10.18502/ijml.v9i4.11619","url":null,"abstract":"Background and Aims: Hypertyrosinemia type 3 (HT3) is an inherited error in tyrosine metabolism caused by a mutation in the 4-hydroxyphenylpyruvate dioxygenase (HPD) gene. Here we report a one and half-year-old girl infant who was diagnosed based on increased serum tyrosine levels and increased urinary excretion of p-hydroxyphenyl derivatives. \u0000Materials and Methods: The proband was one and half-year-old Iranian girl who was diagnosed based on increased serum tyrosine levels and increased urinary excretion of p-hydroxyphenyl derivatives. In this study, we used Whole-Exome Sequencing to identify the genetic reason for the disease and the funded mutation confirmed by Sanger sequencing. \u0000Results and Conclusion: Through whole-exome sequencing screening of heterozygotes c.413C>T (p.T138M) and c.75G.A (p.W25Ter) in the HPD gene and genetically confirmed by Sanger sequencing. There were heterozygous conditions c.413C>T (p.T138M) and c.75G.A (p.W25Ter) in father and mother respectively. This mutation in her parents was also confirmed by Sanger sequencing.","PeriodicalId":183358,"journal":{"name":"International Journal of Medical Laboratory","volume":"188 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131592654","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Differential Expression of Circulating miRNA-103 in Women with and without Polycystic Ovary Syndrome 多囊卵巢综合征女性与非多囊卵巢综合征女性循环miRNA-103的差异表达

International Journal of Medical Laboratory Pub Date : 2023-01-03 DOI: 10.18502/ijml.v9i4.11624

Mohammad Reza Naseri, S. Kalantar, N. Nikoonahad, H. Shafienia, F. Montazeri

{"title":"Differential Expression of Circulating miRNA-103 in Women with and without Polycystic Ovary Syndrome","authors":"Mohammad Reza Naseri, S. Kalantar, N. Nikoonahad, H. Shafienia, F. Montazeri","doi":"10.18502/ijml.v9i4.11624","DOIUrl":"https://doi.org/10.18502/ijml.v9i4.11624","url":null,"abstract":"Background and Aims: Polycystic ovary syndrome (PCOS) has a largely unknown etiology and is a common heterogeneous endocrine disorder in women of reproductive age, characterized by polycystic ovaries, hyperandrogenism, insulin resistance, and chronic anovulation. MicroRNAs (miRNAs) are small, noncoding RNA sequences that negatively regulate gene expression at the post-transcriptional level. miRNA-103 has been associated with metabolic disorders such as obesity and diabetes, which are also associated with PCOS. This study aims to describe the different expressions of circulating miR-103 in the plasma of PCOS women. \u0000Materials and Methods: This case-control study includes 25 women with PCOS and 25 healthy women. Plasma total RNA was isolated and, after polyadenylation, converted to total cDNA. Then, the expression level of microRNA-103 was analyzed by quantitative reverse transcription-polymerase chain reaction, and SNORD miRNA was used as the reference gene. \u0000Results: Our results indicate that the expression level of miRNA-103 significantly decreased in the PCOS group compared to healthy women, which can be related to the etiology and progression of PCOS (p < 0.05). \u0000Conclusions: Our findings recommend further studies in the larger statistical population and more accurate techniques to confirm microRNA-103 as a noninvasive diagnosis biomarker.","PeriodicalId":183358,"journal":{"name":"International Journal of Medical Laboratory","volume":"150 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121041158","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0