International Journal of Medical Laboratory最新文献_第3页

Genetic Variation in Mitochondrial COX1 and Ribosomal ITS2 Genes of Toxocara Canis in Stray Dogs in Zabol, Southeast Iran 伊朗东南部Zabol流浪狗犬弓形虫线粒体COX1和核糖体ITS2基因的遗传变异

International Journal of Medical Laboratory Pub Date : 2023-01-03 DOI: 10.18502/ijml.v9i4.11623

E. Sarani, Maryam Hataminejad, H. Azizi

{"title":"Genetic Variation in Mitochondrial COX1 and Ribosomal ITS2 Genes of Toxocara Canis in Stray Dogs in Zabol, Southeast Iran","authors":"E. Sarani, Maryam Hataminejad, H. Azizi","doi":"10.18502/ijml.v9i4.11623","DOIUrl":"https://doi.org/10.18502/ijml.v9i4.11623","url":null,"abstract":"Background and Aims: Toxocariasis is a zoonotic parasitic disease with worldwide distribution caused by the larval stage of ascaridoid nematodes of dogs (Toxocara canis) and cats (Toxocara cati). The study was accomplished to determine the sequence variation in ITS2 and COX1 genes within isolates of Toxocara canis. \u0000Materials and Methods: Two hundred Stool samples were collected randomly from dogs in public parks and streets from different regions of Zabol. Thirty samples containing eggs were isolated from the feces using Formalin ether 10% and centrifugal flotation. Genomic DNA was extracted, and COX1 and ITS2 were amplified by PCR-RFLP and sequenced. Sequence data were aligned using the BioEdit software and BLAST program and compared with published sequences in GenBank. The phylogenetic relationship between isolates of T. canis from Zabol city with other regions based on sequences obtained from COX1 and ITS2 genes and using MEGA7.0 software was investigated. \u0000Results: For all samples, amplicons of about 388 and 422 base pairs were produced by PCR for ITS2 and COX1, respectively. Drawing the phylogenic tree of ITS2 and COX1 sequences of isolates of T. canis showed that the identified genotypes are not only different from each other but also from other parts of the world. \u0000Conclusions: Our result showed that genetic variation among isolates of T. canis from Zabol is very low. For a deeper understanding of genetic diversity among populations of Toxocara, it is recommended to analyze more isolates from various geographical areas and variable genetic markers.","PeriodicalId":183358,"journal":{"name":"International Journal of Medical Laboratory","volume":"4 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125784260","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Induced Chondrogenic Differentiation of hESCs by hESC-Derived MSCs Conditioned Medium and Sequential 3D-2D Culture System hesc来源的MSCs条件培养基和顺序3D-2D培养系统诱导hesc成软骨分化

International Journal of Medical Laboratory Pub Date : 2023-01-03 DOI: 10.18502/ijml.v9i4.11620

Shayesteh Shahriary, Ehsan Farashahi-Yazd, Fatemeh Hajizadeh-Tafti, F. Akyash, B. Aflatoonian

{"title":"Induced Chondrogenic Differentiation of hESCs by hESC-Derived MSCs Conditioned Medium and Sequential 3D-2D Culture System","authors":"Shayesteh Shahriary, Ehsan Farashahi-Yazd, Fatemeh Hajizadeh-Tafti, F. Akyash, B. Aflatoonian","doi":"10.18502/ijml.v9i4.11620","DOIUrl":"https://doi.org/10.18502/ijml.v9i4.11620","url":null,"abstract":"Background and Aims: It has been proven that human mesenchymal stem cells (MSCs) conditioned medium (hMSCs-CM) can influence human embryonic stem cells (hESCs) chondrogenic differentiation. In this study, we hypothesized that conditioned medium (CM) from hESCs-derived MSCs in a sequential 3D-2D culture system could facilitate the induction of chondrogenesis in hESCs. \u0000Materials and Methods: CM was collected from Yazd2 (hESCs; 46, XY) derived MSCs confluent cultures and stored at -20 °C. Yazd4 hESC line (46, XX) was induced for differentiation using EB formation as 3D culture into SD (spontaneously differentiation) and CM groups (differentiation using conditioned medium) for four days. Cell culture continued in a 2D (monolayer) culture system for both groups till day 14. Ultimately, chondrogenic differentiation was assessed by Alcian blue and masson′s trichrome staining at 4 and 14 days of differentiation, and quantitative real-time polymerase chain reaction (PCR) for NANOG, MEOX1, SOX5, SOX6, SOX9, ACAN, COL2A1 and RUNX2 genes for SD and CM groups on days 0 and 4. \u0000Results: The gene expression profile for chondrogenic genes in the CM group was significantly more than the SD group (p< 0.05). Furthermore, chemical staining assessment illustrated a significant GAG and collagen II difference between the CM and SD groups at days 4 and 14 (p< 0.05). \u0000Conclusions: Our findings would pave the way for creating an in vitro human chondrogenesis model for further studies in the developmental biology of articular cartilage tissue, which lend itself to cell-based therapy to cure joint diseases such as osteoarthritis.","PeriodicalId":183358,"journal":{"name":"International Journal of Medical Laboratory","volume":"22 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125043475","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mutational Screening in Exon 6 of the PSEN2 Gene in Iranian Patients with Late-Onset Alzheimer's Disease 伊朗迟发性阿尔茨海默病患者PSEN2基因外显子6突变筛查

International Journal of Medical Laboratory Pub Date : 2023-01-03 DOI: 10.18502/ijml.v9i4.11625

S. Salari, S. Miresmaeili, Mahta Mazaheri Naeini

{"title":"Mutational Screening in Exon 6 of the PSEN2 Gene in Iranian Patients with Late-Onset Alzheimer's Disease","authors":"S. Salari, S. Miresmaeili, Mahta Mazaheri Naeini","doi":"10.18502/ijml.v9i4.11625","DOIUrl":"https://doi.org/10.18502/ijml.v9i4.11625","url":null,"abstract":"Background and Aims: One of the most important genes involved in Alzheimer's disease (AD) is the presenilin2 (PSEN2) gene, which is one of the main constituents of the gamma-secretase complex. Mutations in this gene promote the formation of amyloid plaques resulting in AD. The study aimed to evaluate the mutation variant in exon 6 of the PSEN2 gene in patients with Late-Onset Alzheimer's disease (LOAD). Due to the important role of the PSEN2 gene in the formation of beta-amyloid aggregates and the investigation involves an association between PSEN2 mutations and their pathogenicity in LOAD progression, we presented this exon as a more efficient alternative. \u0000Materials and Methods: The thirty patients with LOAD and 16 healthy subjects as a control group were involved in this experimental study. DNA was extracted from blood samples and purified. The desired gene fragment was propagated using polymerase chain reaction and the products were electrophoresed and the results were analyzed. \u0000Result: A novel mutation was found in PSEN2 IVS6 + 30 G → C at the intron region of exon 6 in 20 cases of patients suffering from LOAD and 12 subjects in control cohort. In this mutation a guanine base was substituted by cytosine base which this position was 30 nucleotides separated from coding region. \u0000Conclusions: The novel mutation was identified in both studied groups. These findings reveal no relationship between PSEN2 mutation and pathogenicity of LOAD disease. However, further studies are required to find the role of PSEN2 mutation in LOAD progression.","PeriodicalId":183358,"journal":{"name":"International Journal of Medical Laboratory","volume":"19 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115037422","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correlation of Tissue Distribution of HLA-G and HLA-E and Degree of Tumor Malignancy in Patients with Breast Cancer 乳腺癌患者HLA-G、HLA-E组织分布与肿瘤恶性程度的相关性研究

International Journal of Medical Laboratory Pub Date : 2023-01-03 DOI: 10.18502/ijml.v9i4.11626

Mahdieh Jaafari, H. Hadinedoushan, Mahmoud Akhavan Tafti

{"title":"Correlation of Tissue Distribution of HLA-G and HLA-E and Degree of Tumor Malignancy in Patients with Breast Cancer","authors":"Mahdieh Jaafari, H. Hadinedoushan, Mahmoud Akhavan Tafti","doi":"10.18502/ijml.v9i4.11626","DOIUrl":"https://doi.org/10.18502/ijml.v9i4.11626","url":null,"abstract":"Background and Aims: Human leukocyte antigen (HLA) molecules play a substantial role in T Lymphocyte-mediated adaptive immune response. Down-regulation of HLA expression may help the tumor to escape from immune surveillance. This study aimed to evaluate the correlation between HLA-G and HLA-E tissue distribution and the degree of tumor malignancy in human breast tumors. \u0000Materials and Methods: This cross-sectional study was conducted on tissue samples of 145 patients with breast cancer. The distribution of HLA-G and HLA-E expressions were measured by the immunohistochemistry method. \u0000Results: Among 145 patients, 51% of tumors did not express HLA-E, and +1 positive was seen in 36.6 % of patients. +2 positive in 8.3% and +3 positive in 4.1% of patients. Moreover, 79.3% of tumors did not express HLA-G, 17.9% expressed +1 positive, 2.8% expressed +2 positive, and no patients expressed +3 positive. Generally, 20.7% and 49% of patients showed expression of HLA-G and HLA-E, respectively. A significant correlation was seen between the grade of disease and expression of HLA-E (p = 0.03) and HLA-G (p = 0.001). Moreover, a significant correlation was seen between the simultaneous expression of HLA-G and HLA-E with grade (p = 0.03, r = 0.176). \u0000Conclusion: Significant correlation was seen between the distribution of HLA-G and HLA-E expression with a degree of malignancy. Therefore, these biomarkers’ expression may contribute to the prognosis and progression of breast cancer.","PeriodicalId":183358,"journal":{"name":"International Journal of Medical Laboratory","volume":"25 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133012550","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antiviral Activity of Arnebia Euchroma and Amniotic Membrane against HSV-1, Rotavirus, and Influenza Virus 紫草和羊膜对HSV-1、轮状病毒和流感病毒的抗病毒活性

International Journal of Medical Laboratory Pub Date : 2022-10-22 DOI: 10.18502/ijml.v9i3.10910

M. Saghafi, A. Tavakoli, S. Ahmadi, S. Monavari

{"title":"Antiviral Activity of Arnebia Euchroma and Amniotic Membrane against HSV-1, Rotavirus, and Influenza Virus","authors":"M. Saghafi, A. Tavakoli, S. Ahmadi, S. Monavari","doi":"10.18502/ijml.v9i3.10910","DOIUrl":"https://doi.org/10.18502/ijml.v9i3.10910","url":null,"abstract":"Background and Aims: Currently, there are antiviral chemicals used to treat viral infections accompanied by limitations such as high levels of toxicity and adverse effects in humans, the emergence of drug-resistant viral strains, low numbers, and limited diversity. Therefore, it is necessary to evaluate new photochemical to obtain new therapeutic methods. The present study was conducted to evaluate the antiviral activity of Arnebia Euchroma (A. Euchroma) extract, amniotic membrane, a mixture of A. euchroma extract and amniotic membrane and its carrier (comprised of Sesam and ostrich oil) against three different viruses, including Herpes simplex virus type 1 (HSV-1), influenza A, and rotavirus in-vitro. \u0000Materials and Methods: A methyl thiazolyl tetrazolium (MTT) assay was performed to evaluate the cytotoxic effect of the above compounds on Vero, MDCK, and MA-104 cell lines. After the determination of non-toxic concentrations, Tissue culture infection dose 50 (TCID50) test was performed to determine antiviral activity. \u0000Results: Among all the above-mentioned compounds, the combination of A. euchroma extract and amniotic membrane at the highest non-toxic concentration for the cells had the highest antiviral activity against all three viruses, leading to 1 log10 TCID50 reduction in influenza A and HSV-1 titers and 0.6 log10 TCID50 reductions in rotavirus titer when compared to the virus control. \u0000Conclusions: The combination of A. euchroma and amniotic membrane can be considered a new antiviral agent to treat viral infections.","PeriodicalId":183358,"journal":{"name":"International Journal of Medical Laboratory","volume":"4 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"117142921","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Missense Mutation in the HMNT Gene Responsible for Autosomal Recessive Intellectual Disability in an Iranian Family with Consanguineous Marriage 一个伊朗近亲婚姻家庭中导致常染色体隐性智力残疾的HMNT基因错义突变

International Journal of Medical Laboratory Pub Date : 2022-10-18 DOI: 10.18502/ijml.v9i3.10907

Seyed Hamidreza Mirabutalebi, M. Dehghani, N. Ghasemi, Mohammad yahya Vahidi Mehjardi, Mojtaba Movahedinia, S. Kalantar

{"title":"A Missense Mutation in the HMNT Gene Responsible for Autosomal Recessive Intellectual Disability in an Iranian Family with Consanguineous Marriage","authors":"Seyed Hamidreza Mirabutalebi, M. Dehghani, N. Ghasemi, Mohammad yahya Vahidi Mehjardi, Mojtaba Movahedinia, S. Kalantar","doi":"10.18502/ijml.v9i3.10907","DOIUrl":"https://doi.org/10.18502/ijml.v9i3.10907","url":null,"abstract":"Background and Aims: One of the neurotransmitters in the brain is Histamine which acts as several biological mechanism regulators like inflammation, gastric acid secretion, and neuromodulation. Inactivation of Histamine occurs by histamine N-methyltransferase (HNMT) enzyme. The HNMT transfers a methyl group from S-adenosyl-L-methionine to Histamine and is the main process for the termination of neurotransmission actions of Histamine in the mammalian central nervous system. \u0000Materials and Methods: In this case, a family was referred to the genetic clinic to diagnose the cause of their disorder. The clinical form, pedigree, and questionnaire were completed for the family, and the parents gave their written consent for all tests and photographs publication. Both siblings have moderate learning and intellectual disability. Whole exome sequencing was performed and Sanger sequencing for co-segregation was used. \u0000Results: Bioinformatics analysis revealed a homozygous missense variant in HNMT (c.623T>C p.Leu208Pro) which causes non-syndromic autosomal recessive intellectual disability in this consanguineous family. Analysis of segregation confirmed this mutation. P.Leu208Pro mutation reduces the stability of the protein, which reduces the inactivation of Histamine. \u0000Conclusion: HNMT should be considered an important gene in the genetic evaluation of consanguineous families with intellectual disability.","PeriodicalId":183358,"journal":{"name":"International Journal of Medical Laboratory","volume":"34 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124468804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Soil Actinomycetes-derived Secondary Metabolites-Induced Apoptosis in Human Lung Cancer Cells 土壤放线菌衍生的次生代谢物诱导人肺癌细胞凋亡

International Journal of Medical Laboratory Pub Date : 2022-10-18 DOI: 10.18502/ijml.v9i3.10908

Rahil Norbakhsh, Tohid Moradi Gardeshi, Sina Dalvand, Zahra Boroughani

{"title":"Soil Actinomycetes-derived Secondary Metabolites-Induced Apoptosis in Human Lung Cancer Cells","authors":"Rahil Norbakhsh, Tohid Moradi Gardeshi, Sina Dalvand, Zahra Boroughani","doi":"10.18502/ijml.v9i3.10908","DOIUrl":"https://doi.org/10.18502/ijml.v9i3.10908","url":null,"abstract":"Background and Aims: Natural compounds derived from animal, plant, and microbial sources participate in treating various types of cancers, including lung cancer. This survey attempted to explore the anticancer activity of two novel metabolites extracted from soil-derived actinomycetes in the human lung cancer A549 cells. \u0000Materials and Methods: The crude extracts of UTMC 638 and UTMC 877 secondary metabolites were obtained from the University of Tehran Microorganisms Collection (UTMC). When doxorubicin was applied as a positive control, cell viability, apoptosis detection, and mRNA expression were assessed by MTT assay, flow cytometry, and reverse transcription-quantitative polymerase chain reaction (RT-qPCR) technique. \u0000Results: The results of the MTT assay showed that UTMC 638, UTMC 877, and doxorubicin reduce A549 cell viability in a concentration-dependent manner. Cell treatment with UTMC 877, UTMC 638, and doxorubicin could promote apoptosis in the A549 cell line. However, the effect of UTMC 638 on apoptotic induction was more than doxorubicin or UTMC 877. The q-RT-PCR results highlighted that the gene expression associated with apoptosis was augmented in the treated group compared to the untreated group. \u0000Conclusion: Our findings provide evidence that the crude extract of UTMC 676 could promote apoptosis in A549 cells and can be a very promising source for designing a potent antitumor agent against lung cancer cells. ","PeriodicalId":183358,"journal":{"name":"International Journal of Medical Laboratory","volume":"47 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114075769","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Potential Therapeutic Effect of TLR4-Primed Mesenchymal Stem Cells in Lessening Kidney Damages in Rat Model of Diabetic Nephropathy tlr4诱导间充质干细胞减轻糖尿病肾病大鼠肾损伤的潜在治疗作用

International Journal of Medical Laboratory Pub Date : 2022-10-18 DOI: 10.18502/ijml.v9i3.10903

Mohammad Tollabi, N. Ghasemzadeh, Ali Dehghani Firoozabadi

{"title":"Potential Therapeutic Effect of TLR4-Primed Mesenchymal Stem Cells in Lessening Kidney Damages in Rat Model of Diabetic Nephropathy","authors":"Mohammad Tollabi, N. Ghasemzadeh, Ali Dehghani Firoozabadi","doi":"10.18502/ijml.v9i3.10903","DOIUrl":"https://doi.org/10.18502/ijml.v9i3.10903","url":null,"abstract":"Background and Aims: Substantial damage to the kidney tissue and diabetic nephropathy (DN) can be caused by chronic hyperglycemic conditions and exposure to a high level of blood glucose. In the current study, we explored the capability of adipose-derived mesenchymal stem cells (ADSCs) and Toll-like receptor-4-primed mesenchymal stem cells (TLR4-primed MSCs) on kidney regeneration, resolution of inflammation, and alleviation of diabetic nephropathy in DN in the rats. \u0000Materials and Methods: STZ-induced diabetic rat models were divided into 5 subgroups, including 1) DN group, 2) DN group received insulin, 3) DN group received human foreskin fibroblast (DN-HFF), 4) DN group received single pulse of 1×106 cells of MSCs and 5) DN group received single pulse of TLR4-primed MScs. Thereafter, biochemical and histological analysis was performed on DN-model groups. Biochemical analysis exhibited a blood urea nitrogen level recovery in both MSCs and TLR4-primed MSCs-treated groups. Administration of MSCs also up-regulated mRNA expression of Bcl-xL, while the expression of Bax was significantly down-regulated. \u0000Results: Histological and molecular results showed that TLR4-primed MSCs have an anti-inflammatory and anti-apoptotic effect on inflamed kidneys and effectively reduced DN indicators in the TLR4-primed MSCs group compared to the unprimed MSCs group. \u0000Conclusion: Priming with LPS improves the therapeutic effects of MSCs in the rat model of DN, consequently lessening the symptoms of DN rats. This study proposes that primed MSCs can be served as a potential therapeutic approach in diabetes mellitus and DN management.","PeriodicalId":183358,"journal":{"name":"International Journal of Medical Laboratory","volume":"16 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125009703","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of First Patient With Rh Null Phenotype in Southeast Iran 伊朗东南部首例Rh空表型患者的鉴定

International Journal of Medical Laboratory Pub Date : 2022-10-18 DOI: 10.18502/ijml.v9i3.10902

H. Bahramian, Jasem Hashemzehi, Mahmood Nayebzadeh, S. Khosravi

{"title":"Identification of First Patient With Rh Null Phenotype in Southeast Iran","authors":"H. Bahramian, Jasem Hashemzehi, Mahmood Nayebzadeh, S. Khosravi","doi":"10.18502/ijml.v9i3.10902","DOIUrl":"https://doi.org/10.18502/ijml.v9i3.10902","url":null,"abstract":"Background and Aims: Rhnull, with an estimated incidence of one per 6,000,000 individuals, is an extremely rare disorder with an autosomal recessive pattern of inheritance that is more common in societies with a high rate of consanguinity. \u0000Materials and Methods: In this study, we report the first case with Rhnull, a blood group phenotype in southeast Iran, which was diagnosed during pretransfusion testing. \u0000Results and Conclusions: A 21-year-old woman with a positive parents' consanguineous marriage was found to have an unusual reaction with all packed red blood cell units during routine pretransfusion cross-match testing in the hospital. The patient's serum was reacted with all screening and identification panel cells, suspected to have an alloantibody against a common antigen or multiple alloantibodies against her absence antigens. Further studies revealed negative results for C, c, E, and e, which are highly suspected of Rhnull phenotype. Confirmatory assessments were performed, including adsorption and elution studies and Rh phenotyping of patients, along with known positive and negative controls. Due to the blood requirement of the patient, we performed serological studies on the patient's family members and found that her sister also has a Rhnull phenotype. Blood transfusion from her sister's donated units was performed, and the pregnancy was ended without any complications. Finally, due to the rarity of the Rhnull phenotype, early identification of individuals and autologous or compatible allogeneic blood transfusion should be planned prior to selective or emergency surgeries.","PeriodicalId":183358,"journal":{"name":"International Journal of Medical Laboratory","volume":"31 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125753199","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Effects of Atorvastatin Consumption on Biochemical Variables in Patients with Type 2 Diabetes Mellitus and Pre-diabetes 阿托伐他汀对2型糖尿病及糖尿病前期患者生化指标的影响

International Journal of Medical Laboratory Pub Date : 2022-10-18 DOI: 10.18502/ijml.v9i3.10905

Ali Nosrati Andevari, S. Moein, D. Qujeq, Z. Moazezi, K. Hajian-Tilaki

{"title":"The Effects of Atorvastatin Consumption on Biochemical Variables in Patients with Type 2 Diabetes Mellitus and Pre-diabetes","authors":"Ali Nosrati Andevari, S. Moein, D. Qujeq, Z. Moazezi, K. Hajian-Tilaki","doi":"10.18502/ijml.v9i3.10905","DOIUrl":"https://doi.org/10.18502/ijml.v9i3.10905","url":null,"abstract":"Background and Aims: Atorvastatin may alter glycemic traits and lipid profiles. This study aimed to evaluate the effects of atorvastatin on biochemical variables in patients with type 2 diabetes and pre-diabetes (borderline diabetes). \u0000Materials and Methods: This study included 80 individuals divided into five groups. Patients with type 2 diabetes mellitus and pre-diabetes used atorvastatin 20 mg/day for three months. After three months, variables such as serum fasting blood glucose (FBS), cholesterol, triglyceride, low-density lipoprotein-cholesterol (LDL-C), high-density lipoprotein-cholesterol (HDL-C), and glycosylated hemoglobin (HbA1c) levels were measured to assess the status of diabetes and pre-diabetes condition. Linear regression was applied to determine the association between atorvastatin uses and alters of biochemical variables levels. \u0000Results: The serum FBS and HbA1c levels in patients with diabetes and pre-diabetes who use atorvastatin were significantly lower than in patients with diabetes and pre-diabetes who did not use atorvastatin (p=0.001). Serum cholesterol and LDL-C levels decreased in diabetic and pre-diabetic patients who used atorvastatin in comparison with diabetic and pre-diabetic patients who did not use atorvastatin (p=0.001). In patients with pre-diabetes, the use of atorvastatin slightly increased serum HDL-C levels. However, in patients with diabetes, the use of atorvastatin slightly decreased serum HDL-C level (p= 0.001). Diabetic and pre-diabetic patients who use atorvastatin significantly decreased serum triglyceride levels (p=0.016), while in diabetic and pre-diabetic patients, using atorvastatin slightly increased the serum insulin level (p= 0.003). \u0000Conclusions: Atorvastatin using alters fat and sugar indices in diabetic and pre-diabetic patients.","PeriodicalId":183358,"journal":{"name":"International Journal of Medical Laboratory","volume":"40 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114081278","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0