Soil Actinomycetes-derived Secondary Metabolites-Induced Apoptosis in Human Lung Cancer Cells

Rahil Norbakhsh, Tohid Moradi Gardeshi, Sina Dalvand, Zahra Boroughani
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Abstract

Background and Aims: Natural compounds derived from animal, plant, and microbial sources participate in treating various types of cancers, including lung cancer. This survey attempted to explore the anticancer activity of two novel metabolites extracted from soil-derived actinomycetes in the human lung cancer A549 cells. Materials and Methods: The crude extracts of UTMC 638 and UTMC 877 secondary metabolites were obtained from the University of Tehran Microorganisms Collection (UTMC). When doxorubicin was applied as a positive control, cell viability, apoptosis detection, and mRNA expression were assessed by MTT assay, flow cytometry, and reverse transcription-quantitative polymerase chain reaction (RT-qPCR) technique. Results: The results of the MTT assay showed that UTMC 638, UTMC 877, and doxorubicin reduce A549 cell viability in a concentration-dependent manner. Cell treatment with UTMC 877, UTMC 638, and doxorubicin could promote apoptosis in the A549 cell line. However, the effect of UTMC 638 on apoptotic induction was more than doxorubicin or UTMC 877. The q-RT-PCR results highlighted that the gene expression associated with apoptosis was augmented in the treated group compared to the untreated group. Conclusion: Our findings provide evidence that the crude extract of UTMC 676 could promote apoptosis in A549 cells and can be a very promising source for designing a potent antitumor agent against lung cancer cells.   
土壤放线菌衍生的次生代谢物诱导人肺癌细胞凋亡
背景和目的:从动物、植物和微生物中提取的天然化合物参与治疗各种类型的癌症,包括肺癌。本研究旨在探讨从土壤来源的放线菌中提取的两种新型代谢物在人肺癌A549细胞中的抗癌活性。材料与方法:UTMC 638和UTMC 877次生代谢产物粗提物来源于德黑兰大学微生物收集(UTMC)。当阿霉素作为阳性对照时,采用MTT法、流式细胞术和逆转录-定量聚合酶链反应(RT-qPCR)技术评估细胞活力、凋亡检测和mRNA表达。结果:MTT实验结果显示,UTMC 638、UTMC 877和阿霉素均能降低A549细胞活力,且呈浓度依赖性。UTMC 877、UTMC 638和阿霉素处理细胞可促进A549细胞凋亡。但UTMC 638诱导凋亡的作用强于阿霉素和UTMC 877。q-RT-PCR结果显示,与未治疗组相比,治疗组与凋亡相关的基因表达增加。结论:utmc676粗提物具有促进A549细胞凋亡的作用,为设计一种有效的抗肺癌药物提供了依据。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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