Medical mycology journal最新文献_第2页

A Case of Tinea Faciei due to Trichophyton indotineae with Steroid Rosacea Related to Topical Over-The-Counter Drugs Purchased Outside of Japan. 一例由 indotineae 毛癣菌引起的面部癣菌伴有类固醇红斑痤疮，与在日本境外购买的外用非处方药有关。

IF 1

Medical mycology journal Pub Date : 2024-01-01 DOI: 10.3314/mmj.23-00014

Norihito Fukada, Hisashi Kobayashi, Manabu Nakazono, Keita Ohyachi, Akinori Takeda, Takashi Yaguchi, Miyuki Okada, Tomotaka Sato

引用次数: 0

[Aspergillus Cell Surface Structural Analysis and Its Applications to Industrial and Medical Use]. [曲霉细胞表面结构分析及其在工业和医疗中的应用]。

IF 1.4

Medical mycology journal Pub Date : 2024-01-01 DOI: 10.3314/mmj.24.007

Ken Miyazawa, Takashi Umeyama, Akira Yoshimi, Keietsu Abe, Yoshitsugu Miyazaki

{"title":"[Aspergillus Cell Surface Structural Analysis and Its Applications to Industrial and Medical Use].","authors":"Ken Miyazawa, Takashi Umeyama, Akira Yoshimi, Keietsu Abe, Yoshitsugu Miyazaki","doi":"10.3314/mmj.24.007","DOIUrl":"https://doi.org/10.3314/mmj.24.007","url":null,"abstract":"The hyphal surface of cells of filamentous fungi is covered with cell wall, which is mainly composed of polysaccharides. Since the cell wall is the first structure to come in contact with the infection host, the environment, and the fungus itself, the elucidation of the cell wall structure and biogenesis is essential for understanding fungal ecology. Among filamentous fungi, the genus Aspergillus is an important group in the industrial, food, and medical fields. It is known that Aspergillus species form hyphal pellets in shake liquid culture. The authors previously found the role of α-1,3-glucan in hyphal aggregation in Aspergillus species. In addition, extracellular polysaccharide galactosaminogalactan contributed to hyphal aggregation as well, and dual disruption of biosynthesis genes of α-1,3-glucan and galactosaminogalactan resulted in complete hyphal dispersion in shake liquid culture. The characteristic of mycelia to form pellets under liquid culture conditions was the main reason why the growth measurement methods used for unicellular organisms could not be applied. We reported that hyphal growth of the dual disruption mutant could be measured by optical density. A real-time plate reader could be used to determine the growth curve of the mycelial growth of the dual disruption mutant. This measurement approach not only provides basic microbiological insights in filamentous fungi, but also has the potential to be applied to high-throughput screening of anti-Aspergillus drugs.","PeriodicalId":18325,"journal":{"name":"Medical mycology journal","volume":null,"pages":null},"PeriodicalIF":1.4,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142109193","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative Study of Three Commercial Blood (1→3)-β-D-glucan Detection Reagents for Invasive Mycosis. 三种商品血(1→3)-β- d -葡聚糖检测试剂对侵袭性真菌病的比较研究。

IF 1

Medical mycology journal Pub Date : 2023-01-01 DOI: 10.3314/mmj.22-00016

Yuki Miyajima, Kentaro Nagaoka, Yusuke Takegoshi, Makito Kaneda, Yushi Murai, Kou Kimoto, Takayuki Kawamura, Akitoshi Ueno, Hitoshi Kawasuji, Koyomi Kawago, Yasutaka Fukui, Ippei Sakamaki, Yoshihiro Yamamoto

{"title":"Comparative Study of Three Commercial Blood (1→3)-β-D-glucan Detection Reagents for Invasive Mycosis.","authors":"Yuki Miyajima, Kentaro Nagaoka, Yusuke Takegoshi, Makito Kaneda, Yushi Murai, Kou Kimoto, Takayuki Kawamura, Akitoshi Ueno, Hitoshi Kawasuji, Koyomi Kawago, Yasutaka Fukui, Ippei Sakamaki, Yoshihiro Yamamoto","doi":"10.3314/mmj.22-00016","DOIUrl":"https://doi.org/10.3314/mmj.22-00016","url":null,"abstract":"Background: β-D-glucan detection is a useful diagnostic indicator of invasive mycosis. However, the differences among the commercial β-D-glucan assays are unclear. Herein, we explored the diagnostic value of various β-D-glucan assay reagents.Methods: This prospective observational study involved 175 eligible patients suspected to have fungal infections. For all participants, culture examinations were conducted with specimens obtained from the infected site (or blood culture), and β-D-glucan was measured using three commercial kits: Wako β-glucan test (Wako), Fungitech G-test MKII \"Nissui\" (MKII), and Fungitech G-test ES \"Nissui\" (ES).Results: A total of 163 participants were included. Among them, 32 cases of invasive mycosis, 34 cases with mycotic colonization infection, and 97 cases with non-fungal infections were confirmed. Regarding the diagnostic value of the commercial kits for invasive mycosis, the areas under the receiver operating characteristic curves were > 0.8 for all the agents. However, on the basis of the cut-off value set by the manufacturer, the sensitivity and specificity of the three kits for definitive invasive infection were 80.0% and 90.6% with Wako, 80.0% and 80.7% with MKII, and 86.7% and 71.8% with ES, respectively. Moreover, the rate of false-positive β-D-glucan elevation detection in patients with negative fungal culture was 9.3% with Wako, 18.6% with MKII, and 23.7% with ES.Conclusion: Despite the high diagnostic value of β-D-glucan detection in invasive fungal infections, caution should be exercised in interpreting the value of the assay reagents.","PeriodicalId":18325,"journal":{"name":"Medical mycology journal","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10829216","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Culture Supernatant from an Actinomycete sp. Affects Biofilm Formation and Virulence Expression of Candida auris. 放线菌培养上清液对金黄色念珠菌生物膜形成和毒力表达的影响。

IF 1

Medical mycology journal Pub Date : 2023-01-01 DOI: 10.3314/mmj.22-00026

Kenshi Yamane, Mamiko Niki, Taishi Tsubouchi, Tetsuya Watanabe, Kazuhisa Asai, Ken-Ichi Oinuma, Arata Sakiyama, Chaogetu Saren, Yuki Matsumoto, Koichi Makimura, Yukihiro Kaneko, Tomoya Kawaguchi

{"title":"A Culture Supernatant from an Actinomycete sp. Affects Biofilm Formation and Virulence Expression of Candida auris.","authors":"Kenshi Yamane, Mamiko Niki, Taishi Tsubouchi, Tetsuya Watanabe, Kazuhisa Asai, Ken-Ichi Oinuma, Arata Sakiyama, Chaogetu Saren, Yuki Matsumoto, Koichi Makimura, Yukihiro Kaneko, Tomoya Kawaguchi","doi":"10.3314/mmj.22-00026","DOIUrl":"https://doi.org/10.3314/mmj.22-00026","url":null,"abstract":"The multidrug-resistant pathogen Candida auris is characterized by its aggregation under certain conditions, which affects its biofilm formation, drug susceptibility, and pathogenicity. Although the innate tendency to aggregate depends on the strain, the mechanism regulating C. auris aggregation remains unclear. We found that the culture supernatant from one of the 95 Actinomyces strains isolated from a deep-sea environment (IMAs2016D-66) inhibited C. auris aggregation. The cells grown in the presence of IMAs2016D-66 exhibited reduced hydrophobicity, biofilm formation, and enhanced proteolytic activity. In addition, the efflux pump activity of the fluconazole-resistant C. auris strain LSEM 3673 was stimulated by IMAs2016D-66, whereas no significant change was observed in the fluconazole-susceptible strain LSEM 0643. As the relationship between aggregative tendency and virulence in C. auris is still unclear, IMAs2016D-66 can serve as a tool for investigating regulatory mechanisms of phenotype switching and virulence expression of C. auris. Understanding of phenotype switching may help us not only to understand the pathogenicity of C. auris, but also to design new drugs that target the molecules regulating virulence factors.","PeriodicalId":18325,"journal":{"name":"Medical mycology journal","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10829218","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[2021 Epidemiological Survey of Dermatomycoses in Japan]. [2021年日本皮肤菌病流行病学调查]。

IF 1

Medical mycology journal Pub Date : 2023-01-01 DOI: 10.3314/mmj.23-00008

Kaori Nakamura, Tomoo Fukuda

{"title":"[2021 Epidemiological Survey of Dermatomycoses in Japan].","authors":"Kaori Nakamura, Tomoo Fukuda","doi":"10.3314/mmj.23-00008","DOIUrl":"https://doi.org/10.3314/mmj.23-00008","url":null,"abstract":"This is a report of the results of the epidemiological survey on dermatomycoses conducted in 2021. A total of 9,442 patients with dermatomycosis were reported for one year. They include 8,151 (86.3%) with dermatophytosis, 796 (8.4%) with candidiasis, 484 (5.1%) with Malassezia infection, and 11 (0.1%) with deep cutaneous mycosis. In order, the most common types of dermatophytoses were tinea pedis (4,195 cases, 2,341 males and 1,854 females), tinea unguium (2,711 cases, 1,509 males and 1,202 females), tinea corporis (674 cases, 445 males and 229 females), tinea cruris (399 cases, 305 males and 94 females), tinea manus (125 cases, 78 males and 47 females), and tinea capitis (47 cases, 25 males and 22 females). The number of cases of tinea pedis and tinea unguium increased during the summer. A higher percentage of patients were aged 80 or older than in previous surveys. These findings may reflect the increasing percentage of elderly patients seen and the superannuation of the population. As in previous surveys, Trichophyton rubrum and Trichophyton interdigitale were the two most frequently isolated species of fungi causing dermatophytoses. Microsporum canis and Trichophyton tonsurans were the two species most often causing tinea capitis.Regarding cutaneous candidiasis, while candidal intertrigo was the most common in previous surveys, diaper candidiasis in the elderly was the most common in this survey. A background check revealed that this was because a facility included a semi-prophylactic approach to address diaper candidiasis occurring within the ward.Malassezia infections by Malassezia folliculitis clearly increased with each survey. The tendency of certain facilities with many reports of Malassezia folliculitis suggests that it is greatly affected by the presence of physicians familiar with the disease.","PeriodicalId":18325,"journal":{"name":"Medical mycology journal","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138460785","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Importance of Accurate Identification and Antifungal Susceptibility Testing of Aspergillus Species in Clinical Settings. 曲霉种类准确鉴定及药敏试验在临床中的重要性。

IF 1

Medical mycology journal Pub Date : 2023-01-01 DOI: 10.3314/mmj.23-004

Keita Takeda, Junko Suzuki, Yuka Sasaki, Akira Watanabe, Katsuhiko Kamei

引用次数: 0

In vitro Azole Susceptibility Testing of Japanese Isolates of Itraconazole-Resistant Dermatophytes. 日本伊曲康唑耐药皮肤菌的体外药敏试验。

IF 1

Medical mycology journal Pub Date : 2023-01-01 DOI: 10.3314/mmj.23-00005

Honoka Nojo, Junichiro Hiruma, Hiromitsu Noguchi, Masataro Hiruma, Kazutoshi Harada, Koichi Makimura, Rui Kano

引用次数: 0

Isolation of Aspergillus udagawae in Canaliculitis: A Case Report. 小管炎中乌达加曲霉的分离1例。

IF 1

Medical mycology journal Pub Date : 2023-01-01 DOI: 10.3314/mmj.23-00004

Yuji Nishihara, Takahito Nakamura, Yuki Sakai, Yumiko Ban, Masato Tashiro, Koichi Izumikawa, Kei Kasahara

引用次数: 0

Interaction of Host Proteins with Cell Surface Molecules of the Pathogenic Yeast Trichosporon asahii. 病原酵母朝日毛丝虫病宿主蛋白与细胞表面分子的相互作用。

IF 1

Medical mycology journal Pub Date : 2023-01-01 DOI: 10.3314/mmj.22-00020

Tomoe Ichikawa, Misato Okumura, Tomoya Mizumura, Minami Hayashi, Momoe Watanabe, Reiko Ikeda, Yoshio Ishibashi

{"title":"Interaction of Host Proteins with Cell Surface Molecules of the Pathogenic Yeast Trichosporon asahii.","authors":"Tomoe Ichikawa, Misato Okumura, Tomoya Mizumura, Minami Hayashi, Momoe Watanabe, Reiko Ikeda, Yoshio Ishibashi","doi":"10.3314/mmj.22-00020","DOIUrl":"https://doi.org/10.3314/mmj.22-00020","url":null,"abstract":"Trichosporon asahii is an invasive pathogenic yeast that infects immunocompromised hosts. Several virulence factors contribute to the fungal infection; however, the factors that contribute to the occurrence of T. asahii infections remain unclear. Since adhesins are typical virulence factors reported for pathogenic fungi, we looked for host proteins that interact with the T. asahii cell surface. T. asahii and Candida albicans were used for screening using a pull-down assay with fetal bovine serum. Serum albumin and elongation factor 2 were identified as the yeast-binding serum proteins. Additionally, we investigated the interactions of the cell surface-associated molecules (CSM) of T. asahii with vitronectin (VTN), fibronectin, fetuin-A, and alpha-1antitrypsin (AAT). The surface plasmon resonance (SPR) method was used to examine the interaction between CSM and human proteins. On the other hand, the pull-down assay was used to examine the interaction between human proteins and the T. asahii cell surface. Serum albumin, AAT, and VTN were found to interact with T. asahii in both SPR and pull-down assays. This study identified several proteins that interact with T. asahii, suggesting that these proteins play a role in infection mechanisms.","PeriodicalId":18325,"journal":{"name":"Medical mycology journal","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9561185","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Kerion Celsi due to Microsporum canis in an Adult Woman, Treated Successfully with Fosravuconazole. 成年女性犬小孢子虫引起的角状细胞瘤用福司伐康唑治疗成功。

IF 1

Medical mycology journal Pub Date : 2023-01-01 DOI: 10.3314/mmj.22-00025

Harunari Shimoyama, Haruka Taira, Kazuo Satoh, Takashi Tamura, Ayaka Yo, Yoshihiro Sei, Koichi Makimura, Yoshihiro Kuwano

{"title":"Kerion Celsi due to Microsporum canis in an Adult Woman, Treated Successfully with Fosravuconazole.","authors":"Harunari Shimoyama, Haruka Taira, Kazuo Satoh, Takashi Tamura, Ayaka Yo, Yoshihiro Sei, Koichi Makimura, Yoshihiro Kuwano","doi":"10.3314/mmj.22-00025","DOIUrl":"https://doi.org/10.3314/mmj.22-00025","url":null,"abstract":"A 64-year-old woman presented with a fist-sized, severely painful lesion with scales, crusts, pustules, erythema with subcutaneous abscess, and hair loss on the left temporal region. Direct microscopic examination revealed a large number of spores around the hair, which indicated ectothrix hair invasion, and some hyphae were also found. Histopathological examination showed significant inflammatory cell infiltration from the dermis to the subcutaneous tissues and into the hair follicles, destruction of the hair follicles with granulomatous reactions, and fungal masses along the hair within the hair follicles. Microsporum canis was identified based on morphological features via culture method and molecular biological analysis of the internal transcribed spacer region DNA sequence. The patient was diagnosed with kerion celsi caused by M. canis. For treatment of kerion celsi, we chose an oral antifungal agent, fosravuconazole (FRVCZ), which has been available since 2018 only in Japan. Clinical symptoms were cured in 12 weeks without scarring. No side effects were observed during oral administration of FRVCZ. The results of our case and several previous reports suggest that FRVCZ is effective in treating various types of dermatomycoses.","PeriodicalId":18325,"journal":{"name":"Medical mycology journal","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9615222","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0