Mass spectrometry最新文献_第2页

Pulsed Nano-Electrospray Ionization Applied to Solutions of Angiotensin II, Bradykinin, and Gramicidin S in Water/Acetonitrile (1/1) with the Addition of 1% Acetic Acid and 10 mM Ammonium Acetate. 将脉冲纳米电喷雾离子化技术应用于水/乙腈（1/1）中的血管紧张素 II、缓激肽和苎麻素 S 溶液，并添加 1% 乙酸和 10 mM 乙酸铵。

Mass spectrometry Pub Date : 2024-01-01 Epub Date: 2024-11-12 DOI: 10.5702/massspectrometry.A0157

Kenzo Hiraoka, Dilshadbek T Usmanov, Satoshi Ninomiya, Stephanie Rankin-Turner, Satoko Akashi

引用次数: 0

Imaging Lipidomics and Metallomics of Brown Rice Cultivars Used for Sake Production. 用于清酒生产的糙米栽培品种的脂质组学和金属组学成像。

Mass spectrometry Pub Date : 2024-01-01 Epub Date: 2024-12-17 DOI: 10.5702/massspectrometry.A0164

Eliza Farestiani, Yoshihiro Tamada, Koji Okuda, Eiichiro Fukusaki, Shuichi Shimma

{"title":"Imaging Lipidomics and Metallomics of Brown Rice Cultivars Used for Sake Production.","authors":"Eliza Farestiani, Yoshihiro Tamada, Koji Okuda, Eiichiro Fukusaki, Shuichi Shimma","doi":"10.5702/massspectrometry.A0164","DOIUrl":"10.5702/massspectrometry.A0164","url":null,"abstract":"Many previous studies have reported various phospholipids and elements that affect sake production; however, it seems to be challenging to investigate individual types in each rice variety due to their high diversity, not to mention their distribution patterns. Since its introduction, mass spectrometry imaging (MSI) has gained attention in various fields as a simple compound visualization technique. The current study highlights the progress of powerful MSI in comprehensively analyzing phospholipids and minerals in brown rice for sake production. Multivariate analysis suggested phospholipids relating to each rice group based on regions of interest. Phospholipid classes connected with embryo and endosperm included fatty acylcarnitine, diacylglycerol, phosphatidylcholine, phosphatidylglycerol, and phosphatidylethanolamine. Meanwhile, the studied rice groups showed the same distribution of the investigated 12 minerals. This is the first study that reports a comprehensive imaging analysis of phospholipids and elements in brown rice for several cultivars for sake production.","PeriodicalId":18243,"journal":{"name":"Mass spectrometry","volume":"13 1","pages":"A0164"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11664313/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142882449","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Determination of Choline-Containing Compounds in Rice Bran Fermented with Aspergillus oryzae Using Liquid Chromatography/Tandem Mass Spectrometry. 利用液相色谱/串联质谱法测定用黑曲霉发酵的米糠中的含胆碱化合物

Mass spectrometry Pub Date : 2024-01-01 Epub Date: 2024-08-08 DOI: 10.5702/massspectrometry.A0151

Masamitsu Maekawa, Anna Iwahori, Masaki Kumondai, Yu Sato, Toshihiro Sato, Nariyasu Mano

{"title":"Determination of Choline-Containing Compounds in Rice Bran Fermented with Aspergillus oryzae Using Liquid Chromatography/Tandem Mass Spectrometry.","authors":"Masamitsu Maekawa, Anna Iwahori, Masaki Kumondai, Yu Sato, Toshihiro Sato, Nariyasu Mano","doi":"10.5702/massspectrometry.A0151","DOIUrl":"10.5702/massspectrometry.A0151","url":null,"abstract":"Choline-containing compounds are essential nutrients for human activity, as they are involved in many biological processes, including cell membrane organization, methyl group donation, neurotransmission, signal transduction, lipid transport, and metabolism. These compounds are normally obtained from food. Fermented brown rice and rice bran with Aspergillus oryzae (FBRA) is a fermented food product derived from rice and rice ingredients. FBRA exhibits a multitude of functional properties with respect to the health sciences. This study has a particular focus on choline-containing compounds. We first developed a simultaneous liquid chromatography/tandem mass spectrometry (LC/MS/MS) analysis method for seven choline-containing compounds. The method was subsequently applied to FBRA and its ingredients. Hydrophilic interaction chromatography (HILIC) and selected reaction monitoring were employed for the simultaneous analysis of seven choline-containing compounds. MS ion source conditions were optimized in positive ion mode, and the product ions derived from the choline group were obtained through MS/MS optimization. Under optimized HILIC conditions, the peaks exhibited good shape without peak tailing. Calibration curves demonstrated high linearity across a 300- to 10,000-fold concentration range. The application of the method to FBRA and other ingredients revealed significant differences between food with and without fermentation. In particular, betaine and α-glycerophosphocholine were found to be highest in FBRA and brown rice malt, respectively. The results indicated that the fermentation processing of rice ingredients results in alterations to the choline-containing compounds present in foods. The developed HILIC/MS/MS method proved to be a valuable tool for elucidating the composition of choline-containing compounds in foods.","PeriodicalId":18243,"journal":{"name":"Mass spectrometry","volume":"13 1","pages":"A0151"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11331278/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142004578","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Method for High Throughput Free Fatty Acids Determination in a Small Section of Bovine Liver Tissue Using Supercritical Fluid Extraction Combined with Supercritical Fluid Chromatography-Medium Vacuum Chemical Ionization Mass Spectrometry. 一种利用超临界流体萃取结合超临界流体色谱-中真空化学电离质谱法高通量测定小块牛肝组织中游离脂肪酸的方法。

Mass spectrometry Pub Date : 2024-01-01 Epub Date: 2024-01-13 DOI: 10.5702/massspectrometry.A0141

Toshinobu Hondo, Yumi Miyake, Michisato Toyoda

{"title":"A Method for High Throughput Free Fatty Acids Determination in a Small Section of Bovine Liver Tissue Using Supercritical Fluid Extraction Combined with Supercritical Fluid Chromatography-Medium Vacuum Chemical Ionization Mass Spectrometry.","authors":"Toshinobu Hondo, Yumi Miyake, Michisato Toyoda","doi":"10.5702/massspectrometry.A0141","DOIUrl":"10.5702/massspectrometry.A0141","url":null,"abstract":"A novel ionization technique named medium vacuum chemical ionization (MVCI) mass spectrometry (MS), which is a chemical ionization using oxonium (H3O+) and hydroxide (OH-) formed from water, has excellent compatibility with the supercritical fluid extraction (SFE)/supercritical fluid chromatography (SFC). We have studied a method to determine free fatty acids (FFAs) in a small section of bovine liver tissue using SFE/SFC-MVCI MS analysis without further sample preparation. A series of FFA molecules interact with the C18 stationary phase, exhibiting broad chromatographic peaks when using a non-modified CO2 as the mobile phase. It can be optimized by adding a small content of methanol to the mobile phase as a modifier; however, it may dampen the ionization efficiency of MVCI since the proton affinity of methanol is slightly higher than water. We have carefully evaluated the modifier content on the ion detection and column efficiencies. The obtained result showed that an optimized performance was in the range of 1 to 2% methanol-modified CO2 mobile phase for both column efficiency and peak intensity. Higher methanol content than 2% degrades both peak intensity and column efficiency. Using optimized SFC conditions, a section of bovine liver tissue sliced for 14 µm thickness by 1 mm square, which is roughly estimated as about 3300 hepatocytes, was applied to determine 18 FFAs amounts for carbon chains of C12-C24. An amount of each tested FFA was estimated as in the range of 0.07 to 2.6 fmol per cell.","PeriodicalId":18243,"journal":{"name":"Mass spectrometry","volume":"13 1","pages":"A0141"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10806282/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139564293","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recent Developments and Application of Mass Spectrometry Imaging in N-Glycosylation Studies: An Overview. 质谱成像在 N-糖基化研究中的最新发展和应用：综述。

Mass spectrometry Pub Date : 2024-01-01 Epub Date: 2024-02-27 DOI: 10.5702/massspectrometry.A0142

Bharath S Kumar

{"title":"Recent Developments and Application of Mass Spectrometry Imaging in N-Glycosylation Studies: An Overview.","authors":"Bharath S Kumar","doi":"10.5702/massspectrometry.A0142","DOIUrl":"10.5702/massspectrometry.A0142","url":null,"abstract":"Among the most typical posttranslational modifications is glycosylation, which often involves the covalent binding of an oligosaccharide (glycan) to either an asparagine (N-linked) or a serine/threonine (O-linked) residue. Studies imply that the N-glycan portion of a glycoprotein could serve as a particular disease biomarker rather than the protein itself because N-linked glycans have been widely recognized to evolve with the advancement of tumors and other diseases. N-glycans found on protein asparagine sites have been especially significant. Since N-glycans play clearly defined functions in the folding of proteins, cellular transport, and transmission of signals, modifications to them have been linked to several illnesses. However, because these N-glycans' production is not template driven, they have a substantial morphological range, rendering it difficult to distinguish the species that are most relevant to biology and medicine using standard techniques. Mass spectrometry (MS) techniques have emerged as effective analytical tools for investigating the role of glycosylation in health and illness. This is due to developments in MS equipment, data collection, and sample handling techniques. By recording the spatial dimension of a glycan's distribution in situ, mass spectrometry imaging (MSI) builds atop existing methods while offering added knowledge concerning the structure and functionality of biomolecules. In this review article, we address the current development of glycan MSI, starting with the most used tissue imaging techniques and ionization sources before proceeding on to a discussion on applications and concluding with implications for clinical research.","PeriodicalId":18243,"journal":{"name":"Mass spectrometry","volume":"13 1","pages":"A0142"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10904931/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140022127","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Simple and Rapid HPLC-ICP-MS Method for the Simultaneous Determination of Cr(III) and Cr(VI) by Combining a 2,6-Pyridinedicarboxylic Acid Pre-Complexation Treatment. 2,6-吡啶二羧酸预络合快速HPLC-ICP-MS同时测定Cr（III）和Cr（VI）的方法

Mass spectrometry Pub Date : 2024-01-01 Epub Date: 2024-12-18 DOI: 10.5702/massspectrometry.A0161

Akane Ito, Kazuto Isamoto, Yuhei Morishita, Masaharu Tanimizu

{"title":"Simple and Rapid HPLC-ICP-MS Method for the Simultaneous Determination of Cr(III) and Cr(VI) by Combining a 2,6-Pyridinedicarboxylic Acid Pre-Complexation Treatment.","authors":"Akane Ito, Kazuto Isamoto, Yuhei Morishita, Masaharu Tanimizu","doi":"10.5702/massspectrometry.A0161","DOIUrl":"10.5702/massspectrometry.A0161","url":null,"abstract":"A simple and rapid analytical method was developed for the simultaneous determination of two chromium species, Cr(III) and Cr(VI), in the environmental waters by high-performance liquid chromatography-inductively coupled plasma-mass spectrometry (HPLC-ICP-MS). This study incorporated a chelating pretreatment with 2,6-pyridinedicarboxylic acid (PDCA) to convert Cr(III) species into a stable Cr(III)-PDCA anion complex, which was then separated from Cr(VI) oxyanion using an anion exchange column. Building on the fundamental analytical approach proposed by Shigeta et al. (doi: 10.2116/analsci.18P012), the mobile phase was optimized to ensure stability for ICP-MS detection, avoiding nonvolatile salts. Chromium species and chloride ions were effectively separated within 6 minutes at a flow rate of 0.6 mL min-1 with the optimized mobile phase, which consisted of 50 mmol L-1 ammonium acetate (pH 6.80) and 2 mmol L-1 PDCA. The detection limits were 0.18 μg L-1 and 0.09 μg L-1 for Cr(III) and Cr(VI), respectively, at m/z 52 under He collision mode.","PeriodicalId":18243,"journal":{"name":"Mass spectrometry","volume":"13 1","pages":"A0161"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11664310/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142882460","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fragmentation Considerations Using Amidoamine Oxide Homologs. 胺胺氧化物同源物的裂解考虑。

Mass spectrometry Pub Date : 2024-01-01 Epub Date: 2024-12-05 DOI: 10.5702/massspectrometry.A0158

Atsushi Yamamoto, Naoji Tokai, Rie Kakehashi, Daisuke Saigusa

{"title":"Fragmentation Considerations Using Amidoamine Oxide Homologs.","authors":"Atsushi Yamamoto, Naoji Tokai, Rie Kakehashi, Daisuke Saigusa","doi":"10.5702/massspectrometry.A0158","DOIUrl":"10.5702/massspectrometry.A0158","url":null,"abstract":"This study investigates the mass spectrometric analysis of 10 novel amidoamine oxide compounds, which are innovative hydrogelators for polar solvents. This research aims to identify characteristic fragment patterns for these amide compounds using high-resolution mass spectrometry. Methanol solutions of the compounds were analyzed in positive and negative ion modes, and MS1 and MS2 spectra at 6 collision energy levels were obtained via electrospray ionization and hybrid tandem mass spectrometry. The importance of low-intensity peaks in structure elucidation was emphasized because low-intensity fragments could provide crucial structural information, especially for compounds with similar structures. Chain-length-dependent fragmentation patterns were observed, which could aid in predicting the structures of related compounds. This research highlights the challenges of balancing informative low-intensity peaks with accurate spectral matching in databases. Based on our results, combining mass spectrometry with separation techniques, such as liquid chromatography, could enhance structural elucidation for unknown compounds. This study contributes to the broader field of mass spectrometry and structural chemistry, particularly in the analysis of amide compounds, and future directions are proposed for developing robust algorithms for selecting and interpreting low-intensity peaks to improve compound identification in complex mixtures.","PeriodicalId":18243,"journal":{"name":"Mass spectrometry","volume":"13 1","pages":"A0158"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11626507/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142801470","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Direct Additive Detection in Polymer Films via Platinum-Assisted SALDI Mass Spectrometry Imaging. 通过铂辅助 SALDI 质谱成像直接检测聚合物薄膜中的添加剂。

Mass spectrometry Pub Date : 2024-01-01 Epub Date: 2024-12-17 DOI: 10.5702/massspectrometry.A0162

Hideya Kawasaki, Issey Osaka, Ryuichi Arakawa

引用次数: 0

Effect of the Surface Morphology of a Metal Film on Ion Yields in a Platinum-Film Surface-Assisted Laser Desorption/Ionization Mass Spectrometry. 金属膜表面形态对铂膜表面辅助激光解吸/电离质谱仪离子产量的影响

Mass spectrometry Pub Date : 2024-01-01 Epub Date: 2024-11-12 DOI: 10.5702/massspectrometry.A0154

Kotaro Hashimoto, Kyosuke Kaneda, Taichi Shimazaki, Chouma Kurihashi, Shuhei Yamamoto, Riko Takata, Shota Nakanishi, Issey Osaka

{"title":"Effect of the Surface Morphology of a Metal Film on Ion Yields in a Platinum-Film Surface-Assisted Laser Desorption/Ionization Mass Spectrometry.","authors":"Kotaro Hashimoto, Kyosuke Kaneda, Taichi Shimazaki, Chouma Kurihashi, Shuhei Yamamoto, Riko Takata, Shota Nakanishi, Issey Osaka","doi":"10.5702/massspectrometry.A0154","DOIUrl":"10.5702/massspectrometry.A0154","url":null,"abstract":"Matrix-assisted laser desorption ionization (MALDI) and surface-assisted laser desorption ionization (SALDI) mass spectrometry (MS), which can detect biomolecules and polymers, are widely used in biochemistry and material science. Some compounds that are difficult to ionize using MALDI can be ionized using SALDI. However, it is difficult to obtain high ion yields using SALDI/MS. In this study, a fabricated platinum (Pt) film with nanostructures on the sample surface using a sputtering method was evaluated to determine the optimal metal film for ion yield in SALDI. The surface morphology of the Pt film was analyzed using scanning electron microscopy (SEM), atomic force microscopy (AFM), transmission electron microscopy (TEM), X-ray reflectivity (XRR), and ultraviolet-visible-near-infrared (UV-Vis-NIR) reflectance spectroscopy. The SEM, AFM, and TEM images of the Pt film showed the deposited metal film giving high ion yields of polyethylene glycol (PEG) in SALDI/MS with a Pt film (Pt-SALDI) that had a rough surface. The densities and reflectivity of films were analyzed by XRR and UV-Vis-NIR. The higher ion yields of PEG were obtained by Pt-SALDI with the Pt films with lower densities and reflectivity. This indicates that the deposition conditions for the Pt films significantly improved the ion yield in Pt-SALDI/MS. The Pt-SALDI has ionization capabilities different from those of MALDI. Therefore, optimization of Pt film for SALDI/MS and the MS imaging allows more compounds to be detected with higher sensitivity.","PeriodicalId":18243,"journal":{"name":"Mass spectrometry","volume":"13 1","pages":"A0154"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11565274/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142648620","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Intermolecular Interactions between Aromatic Amino Acids Investigated by Ultraviolet Photodissociation Spectroscopy of Hydrogen-Bonded Clusters of Histidine and Tryptophan Enantiomers. 组氨酸和色氨酸对映体氢键簇的紫外光解光谱研究芳香氨基酸分子间相互作用。

Mass spectrometry Pub Date : 2024-01-01 Epub Date: 2024-11-26 DOI: 10.5702/massspectrometry.A0160

Keitaro Kitahashi, Akimasa Fujihara

{"title":"Intermolecular Interactions between Aromatic Amino Acids Investigated by Ultraviolet Photodissociation Spectroscopy of Hydrogen-Bonded Clusters of Histidine and Tryptophan Enantiomers.","authors":"Keitaro Kitahashi, Akimasa Fujihara","doi":"10.5702/massspectrometry.A0160","DOIUrl":"https://doi.org/10.5702/massspectrometry.A0160","url":null,"abstract":"Intermolecular interactions between aromatic amino acids were investigated by ultraviolet photodissociation spectroscopy of hydrogen-bonded protonated clusters of histidine (His) and tryptophan (Trp) enantiomers in the gas phase. Product ion spectra and photodissociation spectra in the wavelength range of the S1-S0 transition of Trp at several temperatures (8-100 K) were obtained using a tandem mass spectrometer equipped with an electrospray ionization source and a cold ion trap. l-Trp detachment forming protonated His was the main pathway. Two bands observed at 288 and 285 nm in the photodissociation spectra of heterochiral H+(d-His)(l-Trp) indicated the coexistence of two types of conformers. The bands at 288 and 285 nm were attributed to the conformers formed from stronger and weaker intermolecular interactions, respectively. In the spectra of homochiral H+(l-His)(l-Trp), only the band due to the stronger interactions was observed at 288 nm. The intermolecular interactions of l-His with l-Trp were stronger than those of d-His with l-Trp.","PeriodicalId":18243,"journal":{"name":"Mass spectrometry","volume":"13 1","pages":"A0160"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11606909/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142770395","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0