Mass spectrometry最新文献_第2页

Thermochemical Stabilities of Cluster Ions SiX₃ ⁺(SiX₄)_1,2 (X = H, F and Cl) and SiCl₄ ⁺(SiCl₄)_1,2 Studied by Pulsed-Electron Beam Mass Spectrometry. 脉冲电子束质谱法研究了SiX3 +(SiX4)1,2 （X = H， F和Cl）和SiCl4 +(SiCl4)1,2的热化学稳定性。

Mass spectrometry Pub Date : 2026-01-01 Epub Date: 2026-09-02 DOI: 10.5702/massspectrometry.A0199

Kenzo Hiraoka

{"title":"Thermochemical Stabilities of Cluster Ions SiX3 +(SiX4)1,2 (X = H, F and Cl) and SiCl4 +(SiCl4)1,2 Studied by Pulsed-Electron Beam Mass Spectrometry.","authors":"Kenzo Hiraoka","doi":"10.5702/massspectrometry.A0199","DOIUrl":"10.5702/massspectrometry.A0199","url":null,"abstract":"An investigation on the ion/molecule reactions in SiCl4 provides critical insights into the plasma chemistry of silicon-based materials. In this study, ion/molecule reactions in a gas mixture of SiCl4/N2 were investigated using a pulsed-electron beam mass spectrometer. The primary reaction products from the reactions of N2 + and N4 + with SiCl4 were found to be SiCl3 + and SiCl4 +, respectively. The thermochemical stabilities of SiCl3 +(SiCl4)1,2, SiCl3 +(N2), and SiCl4 +(SiCl4)1,2 were determined. The discontinuous decrease in the bond energies of SiCl3 +(SiCl4) n and SiCl4 +(SiCl4) n between n = 1 and 2 indicates the participation of a covalent bond (i.e., charge transfer) in SiCl3 +(SiCl4) and SiCl4 +(SiCl4). In contrast, the bond in SiCl3 +(N2) was found to be mainly electrostatic.","PeriodicalId":18243,"journal":{"name":"Mass spectrometry","volume":"15 1","pages":"A0199"},"PeriodicalIF":0.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13542414/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148897529","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Direct Detection of Volatile Organic Compounds through Cationization Using ICP-MS/MS. ICP-MS/MS直接检测挥发性有机化合物

Mass spectrometry Pub Date : 2026-01-01 Epub Date: 2026-02-03 DOI: 10.5702/massspectrometry.A0187

Takafumi Hirata, Chihaya Kinoshita, Maki Uezono, Hui Hsin Khoo

{"title":"Direct Detection of Volatile Organic Compounds through Cationization Using ICP-MS/MS.","authors":"Takafumi Hirata, Chihaya Kinoshita, Maki Uezono, Hui Hsin Khoo","doi":"10.5702/massspectrometry.A0187","DOIUrl":"10.5702/massspectrometry.A0187","url":null,"abstract":"We have developed a new analytical technique for the detection of volatile organic compounds (VOCs) using inductively coupled plasma tandem mass spectrometry (ICP-MS/MS). Eight VOCs of various polarities (acetic acid, 2-butanone, pyridine, 2-methylfuran, ethylene, benzene, toluene, and limonene) were introduced into the collision/reaction cell (CRC) of the ICP-MS/MS, bypassing the ICP ion source. This approach enabled a softer ionization than the typical ICP, allowing the detection of the molecules in their intact form. In this study, to explore the potential of cationization as a soft ionization approach, the interaction of the above VOCs with various elements (i.e., Li, Be, Na, Mg, V, Co, Ni, Cu, Zn, As, Rb, Sr, Y, Ru, Pd, Ag, Cd, Cs, Ba, Pt, and Hg) was investigated. The production ratio of cation adducts was evaluated using benzene as a model compound by monitoring [C6H6 + E]+/E+ values (E represents each element). The resulting [C6H6 + E]+/E+ showed a wide variation, covering ranges from 0.0001% to 1%, and the elements with ionization energies between 650 and 900 kJ mol-1 exhibited the highest [C6H6 + E]+/E+ values. The data obtained here revealed that several elements, including Co, Ni, Cu, Ru, Pd, and Ag, can be suitable elements for cationization. The reduction in fragmentation resulted in easier detection of specific compounds based on their m/z values. To demonstrate the practicality of the present technique, several VOCs released from coffee beans through laser-induced evaporation were monitored. The data obtained here envisage a possibility that the detection of VOCs through cationization achieved in ICP-MS/MS can become an effective choice as a rapid analytical tool for VOCs in solid samples.","PeriodicalId":18243,"journal":{"name":"Mass spectrometry","volume":"15 1","pages":"A0187"},"PeriodicalIF":0.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12877431/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146142990","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Instrumental Dispersion Evaluation on Supercritical Fluid Chromatography-Medium Vacuum Chemical Ionization Tandem Mass Spectrometry. 超临界流体色谱-介质真空化学电离串联质谱的仪器色散评价。

Mass spectrometry Pub Date : 2026-01-01 Epub Date: 2026-01-27 DOI: 10.5702/massspectrometry.A0186

Toshinobu Hondo, Yumi Miyake, Michisato Toyoda

{"title":"Instrumental Dispersion Evaluation on Supercritical Fluid Chromatography-Medium Vacuum Chemical Ionization Tandem Mass Spectrometry.","authors":"Toshinobu Hondo, Yumi Miyake, Michisato Toyoda","doi":"10.5702/massspectrometry.A0186","DOIUrl":"10.5702/massspectrometry.A0186","url":null,"abstract":"Instrumental dispersion in the ion source can severely distort fast chromatographic peaks in supercritical fluid chromatography (SFC)-mass spectrometry (MS). Despite this importance, the dispersion characteristics specific to medium-vacuum chemical ionization (MVCI) sources have not been quantitatively investigated. In this work, we combine targeted experiments with established computational tools-computational fluid dynamics (CFD) and electrostatic field simulation-to characterize ion transport in the MVCI flow tube. Arrival profiles of vitamin K1 (VK1) ions monitored by selected ion monitoring consistently showed a reproducible two-component structure consisting of an early narrow bandwidth ion packet followed by a delayed shoulder. CFD calculations reproduce this tailing peak profile, and electrostatic modeling further revealed that applying the same potential to the MVCI flow tube and inner cylinder generates lateral potential walls that inhibit long-residence-time ions from entering the skimmer. Introducing an appropriate potential difference between the MVCI inner cylinder and the skimmer orifice isolates the MVCI flow field from the ion guide region and suppresses long residence-time trajectories, which narrows the VK1 peak width by more than threefold and restores the intrinsic column efficiency of a sub-2-μm SFC column (from the theoretical plate (N) = 3120 to 12079). These results provide a practical diagnostic and mitigation framework for ion-source derived dispersion in MVCI, demonstrating that modest electrostatic confinement is effective in maintaining chromatographic fidelity in high-speed SFC-MVCI-MS. The present work also highlights the utility of CFD for evaluating proposed MVCI flow designs and their influence on peak dispersion.","PeriodicalId":18243,"journal":{"name":"Mass spectrometry","volume":"15 1","pages":"A0186"},"PeriodicalIF":0.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12851858/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146106117","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of Thermal Flash Heating/Rapid Cooling System Using a Heated Needle Coupled with an Alternating Current (AC) Corona Discharge Ion Source. 利用加热针与交流电晕放电离子源耦合的热闪热/快速冷却系统的研制。

Mass spectrometry Pub Date : 2026-01-01 Epub Date: 2026-06-17 DOI: 10.5702/massspectrometry.A0194

Ai Yamashita, Haruo Shimada, Takao Nishiguchi, Kazumasa Kinoshita, Kenzo Hiraoka

引用次数: 0

High Recovery Stop-and-Go Extraction Tips Using Polytetrafluoroethylene Disks Embedded with Poly(styrene-divinylbenzene) Particles for Proteomics. 利用聚四氟乙烯磁盘嵌入聚苯乙烯-二乙烯基苯颗粒进行蛋白质组学的高回收率的走走停停提取提示。

Mass spectrometry Pub Date : 2026-01-01 Epub Date: 2026-02-04 DOI: 10.5702/massspectrometry.A0189

Hiroto Kakiuchi, Eisuke Kanao, Kosuke Ogata, Yasushi Ishihama

{"title":"High Recovery Stop-and-Go Extraction Tips Using Polytetrafluoroethylene Disks Embedded with Poly(styrene-divinylbenzene) Particles for Proteomics.","authors":"Hiroto Kakiuchi, Eisuke Kanao, Kosuke Ogata, Yasushi Ishihama","doi":"10.5702/massspectrometry.A0189","DOIUrl":"10.5702/massspectrometry.A0189","url":null,"abstract":"The stop-and-go extraction tip (StageTip) is widely used for peptide purification in bottom-up proteomics, yet the original 3M Empore disk is no longer available, prompting the need to evaluate current alternatives. Two types of commercially available poly(styrene-divinylbenzene) (SDB)-containing polytetrafluoroethylene (PTFE) disks were used to fabricate StageTips. Desalting was performed on 500 and 20 ng of HeLa tryptic peptides, and the nanoscale liquid chromatography-tandem mass spectrometry results were compared. Both StageTips demonstrated equivalent performance for the 500 ng sample, but for the 20 ng sample, one StageTip identified 1.8 times more peptides, particularly longer and more hydrophobic peptides. Physical characterization and scanning electron microscope imaging of these disks revealed that the high-performing disk contains more PTFE fibers, partially covering the SDB particle surface. This likely prevents hydrophobic peptides from being trapped in small mesopores, improving recovery from low-input samples. These findings demonstrate that disk material properties critically influence performance in trace proteomics.","PeriodicalId":18243,"journal":{"name":"Mass spectrometry","volume":"15 1","pages":"A0189"},"PeriodicalIF":0.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12878562/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146143064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comprehensive Lipidomic Analysis of Recombinant Protein-Producing CHO Cell Cultures: Insights for Media and Process Development. 重组蛋白生产CHO细胞培养的综合脂质组学分析：对媒体和工艺发展的见解。

Mass spectrometry Pub Date : 2026-01-01 Epub Date: 2026-04-17 DOI: 10.5702/massspectrometry.A0192

Shuwen Chen, Esther Kai Lay Peh, Farouq Bin Mahfut, Yuan Sheng Yang, Yin Ying Ho, Ying Swan Ho

{"title":"Comprehensive Lipidomic Analysis of Recombinant Protein-Producing CHO Cell Cultures: Insights for Media and Process Development.","authors":"Shuwen Chen, Esther Kai Lay Peh, Farouq Bin Mahfut, Yuan Sheng Yang, Yin Ying Ho, Ying Swan Ho","doi":"10.5702/massspectrometry.A0192","DOIUrl":"10.5702/massspectrometry.A0192","url":null,"abstract":"Chinese hamster ovary (CHO) cell expression systems are widely utilized for biologics manufacturing due to their efficient recombinant protein expression and human-like post-translational modifications. With an increasing demand for bio-therapeutic proteins, the development of high-yielding CHO cell lines and refining bioprocess parameters are key to achieving reliable, efficient, and cost-effective therapeutic protein production. Many lipidomic studies on CHO cells have highlighted the crucial roles of lipids in supporting cell growth and specific productivity, offering valuable insights for optimizing culture media and metabolic pathways. Among these lipid classes, sterols such as cholesterol and its downstream intermediates have been investigated in CHO cells, though their functions remain less thoroughly explored compared to overall lipid metabolism. In this study, comprehensive lipidomic profiling, including cholesterol and esterified cholesterol, was performed on two distinct basal media and feed conditions, which identified lipid composition changes that offer mechanistic insights into how culture conditions potentially influence membrane dynamics. These findings provide a foundation for future development of culture media and bioprocess control strategies that can ultimately improve yield and process consistency.","PeriodicalId":18243,"journal":{"name":"Mass spectrometry","volume":"15 1","pages":"A0192"},"PeriodicalIF":0.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13093251/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147775559","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mass Spectrometry Imaging of Time-Dependently Photodegraded Light Stabilizers in Polyethylene Films Using Tapping-Mode Scanning Probe Electrospray Ionization. 利用轻敲模式扫描探针电喷雾电离质谱成像聚乙烯薄膜中随时间变化的光降解光稳定剂。

Mass spectrometry Pub Date : 2025-01-01 Epub Date: 2025-06-06 DOI: 10.5702/massspectrometry.A0173

Tsuyoshi Akiyama, Yoichi Otsuka, Mengze Sun, Shinichi Yamaguchi, Michisato Toyoda

{"title":"Mass Spectrometry Imaging of Time-Dependently Photodegraded Light Stabilizers in Polyethylene Films Using Tapping-Mode Scanning Probe Electrospray Ionization.","authors":"Tsuyoshi Akiyama, Yoichi Otsuka, Mengze Sun, Shinichi Yamaguchi, Michisato Toyoda","doi":"10.5702/massspectrometry.A0173","DOIUrl":"10.5702/massspectrometry.A0173","url":null,"abstract":"Light stabilizers are additives that are widely used to improve the lifespan and performance of polymer materials. To develop advanced polymer materials, analytical techniques investigate the degradation mechanisms and distribution of additives in polymers are crucial. Herein, two extraction-ionization methods were used: tapping-mode scanning probe electrospray ionization (t-SPESI) and liquid extraction surface analysis (LESA). The distribution and molecular structure of the photodegradation products were investigated using polyethylene films containing two types of oligomeric hindered amine light stabilizers (o-HALS). In addition, to study the relationship between light irradiation time and the relative amount of photodegradation products, we developed a method for preparing films with multiple photodegradation regions. Mass spectrometry imaging (MSI) using t-SPESI (t-SPESI-MSI) revealed that the signal intensities of HALS decreased with the time of light irradiation, and its degradation products progressively changed. Moreover, tandem mass spectrometry (MS/MS) using LESA (LESA-MS/MS) revealed that degradation products were generated by HALS fragmentation in the polymer film. By integrating these results, we propose multiple and stepwise reactions for the formation of the photodegradation products. Results indicate that the combined use of t-SPESI-MSI and LESA-MS/MS can directly analyze and understand the photodegradation mechanism of o-HALS in polymer materials.","PeriodicalId":18243,"journal":{"name":"Mass spectrometry","volume":"14 1","pages":"A0173"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12146835/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144258323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Stepwise Monitoring of Ligand Exchange on Gold Nanorods: From Cetyltrimethylammonium Bromide to Thiol-Functionalized Biocompatible Phosphorylcholine Using Matrix-Free LDI-TOF Mass Spectrometry. 利用无基质LDI-TOF质谱法逐步监测金纳米棒上的配体交换：从十六烷基三甲基溴化铵到巯基功能化生物相容性磷酸胆碱。

Mass spectrometry Pub Date : 2025-01-01 Epub Date: 2025-12-18 DOI: 10.5702/massspectrometry.A0184

Hideya Kawasaki, Yasuhiko Iwasaki, Ryuichi Arakawa

{"title":"Stepwise Monitoring of Ligand Exchange on Gold Nanorods: From Cetyltrimethylammonium Bromide to Thiol-Functionalized Biocompatible Phosphorylcholine Using Matrix-Free LDI-TOF Mass Spectrometry.","authors":"Hideya Kawasaki, Yasuhiko Iwasaki, Ryuichi Arakawa","doi":"10.5702/massspectrometry.A0184","DOIUrl":"10.5702/massspectrometry.A0184","url":null,"abstract":"Gold nanorods (AuNRs) possess anisotropic optical and electronic properties, primarily determined by their aspect ratio and surface ligands, which make them attractive for applications in sensing, catalysis, and nanomedicine. While these nanorods are typically stabilized using cetyltrimethylammonium bromide (CTAB) to ensure colloidal dispersion, the cytotoxicity and strong surface affinity of CTAB hinder further surface modification through ligand exchange. In this study, we employed matrix-free laser desorption/ionization time-of-flight mass spectrometry (LDI-TOF-MS) to directly monitor the ligand exchange process on AuNRs. This technique enables the detection of intact CTAB, transient intermediates, and final thiol-bound ligands without requiring chemical derivatization. By correlating mass spectral data with ultraviolet-visible-near-infrared absorption and zeta potential measurements, we elucidate a stepwise ligand exchange mechanism in which CTAB is gradually displaced by a thiol-functionalized phosphorylcholine ligand, facilitated by electrostatic interaction with poly(styrene sulfonate). These findings highlight the utility of matrix-free LDI-TOF-MS as a powerful analytical tool for gaining mechanistic insights into ligand exchange reactions at the nanoscale, particularly in aqueous environments.","PeriodicalId":18243,"journal":{"name":"Mass spectrometry","volume":"14 1","pages":"A0184"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12719553/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145819925","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Erratum: Mass Spectrometry as a First-Line Diagnostic Aid for Congenital Disorders of Glycosylation. 勘误：质谱法作为先天性糖基化疾病的一线诊断援助。

Mass spectrometry Pub Date : 2025-01-01 Epub Date: 2025-03-04 DOI: 10.5702/massspectrometry.X0002

Yoshinao Wada

引用次数: 0

Report on the 10th Asia-Oceania Mass Spectrometry Conference (AOMSC2025). 第十届亚洲-大洋洲质谱会议（AOMSC2025）报告。

Mass spectrometry Pub Date : 2025-01-01 Epub Date: 2025-12-18 DOI: 10.5702/massspectrometry.K0013

Yasushi Ishihama

引用次数: 0