Mass Spectrometry Letters最新文献_第10页

The Use of Electrostatic Repulsion-Hydrophilic Interaction Chromatography (ERLIC) for Proteomics Research 静电排斥-亲水性相互作用色谱(ERLIC)在蛋白质组学研究中的应用

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Mass Spectrometry Letters Pub Date : 2014-12-30 DOI: 10.5478/MSL.2014.5.4.95

J. T. Ng, P. Hao, S. Sze

{"title":"The Use of Electrostatic Repulsion-Hydrophilic Interaction Chromatography (ERLIC) for Proteomics Research","authors":"J. T. Ng, P. Hao, S. Sze","doi":"10.5478/MSL.2014.5.4.95","DOIUrl":"https://doi.org/10.5478/MSL.2014.5.4.95","url":null,"abstract":"Characterization and studies of proteome are challenging because biological samples are complex, with a wide dynamic range of abundance. At present the proteins are identified by digestion into peptides, with subsequent identification of the peptides by mass spectrometry (MS). MS is a powerful technique for the purpose, but it cannot identify every peptide in such complex mixtures simultaneously. For accurate analysis and quantification it is important to separate the peptides first by chro- matography into fractions of a size that MS can handle. With these less complex fractions, the probability is increased of identi- fying peptides of low abundance that would otherwise experience ion suppression effects due to the presence of peptides of high abundance. Enrichment for peptides with certain post-translational modifications helps to increase their detection rates as well. Electrostatic repulsion-hydrophilic interaction chromatography (ERLIC) is a mixed-mode chromatographic technique which combines the use of electrostatic repulsion and hydrophilic interaction. This review provides an overview of ERLIC and its var- ious proteomics applications. ERLIC has been demonstrated to have good orthogonality to reverse phase liquid chromatography (RPLC), making it useful as a first dimension in multidimensional liquid chromatography (MDLC) and fractionation of digests in general. Peptides elute in order of their isoelectric points and polarity. ERLIC has also been successfully utilized for the enrichment for phosphopeptides and glycopeptides, facilitating their identification. In addition, it is promising for the study of peptide deamidation. ERLIC performs comparably well or better than established methods for these various applications, and serves as a viable and efficient workflow alternative.","PeriodicalId":18238,"journal":{"name":"Mass Spectrometry Letters","volume":null,"pages":null},"PeriodicalIF":0.5,"publicationDate":"2014-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72786648","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Degradation Efficiency and Characterization of Lincomycin by Electron Beam Irradiation 电子束辐照降解林可霉素的效率及表征

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Mass Spectrometry Letters Pub Date : 2014-09-30 DOI: 10.5478/MSL.2014.5.3.89

Hyun-Sun Ham, Hyun-Woo Cho, S. Myung

{"title":"Degradation Efficiency and Characterization of Lincomycin by Electron Beam Irradiation","authors":"Hyun-Sun Ham, Hyun-Woo Cho, S. Myung","doi":"10.5478/MSL.2014.5.3.89","DOIUrl":"https://doi.org/10.5478/MSL.2014.5.3.89","url":null,"abstract":"Lincomycin is one of the major species among the Pharmaceuticals and Personal Care Products (PPCPs) detected from the four major rivers in Korea. The structure characterization was performed of six degradation products of lincomycin formed under the irradiation of electron beam, and the degradation efficiency as a function of the various irradiation dose and sample concentration was investigated. Electron beam (10 MeV, 0.5 mA and 5 kW) experiments for the structural characteriza- tion of degradation products that are fortified with lincomycin, were performed at the dose of 10 kGy. The separation of degrada- tion products and lincomycin was carried out using a C18 column (2.1×100 mm, 3.5 µm), using gradient elution with 20 mM ammonium acetate and acetonitrile. The structures of six degradation products of lincomycin were proposed by interpretation of mass spectra and chromatograms by LC-MS/MS. The mass fragmentation pathways of mass spectra in tandem mass spectrome- try were also proposed. Experiments were performed of the degradation efficiency as a function of the irradiation dose intensity and the initial concentration of lincomycin in an aqueous environment. In addition, increased degradation efficiency was observed with a higher dose of electron beam and lower concentration.","PeriodicalId":18238,"journal":{"name":"Mass Spectrometry Letters","volume":null,"pages":null},"PeriodicalIF":0.5,"publicationDate":"2014-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81170089","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

A Method to Monitor Dutasteride in Rat Plasma Using Liquid-Liquid Extraction and Multiple Reaction Monitoring: Comparisons and Validation 液-液萃取和多重反应监测大鼠血浆中杜他雄胺的方法:比较与验证

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Mass Spectrometry Letters Pub Date : 2014-09-30 DOI: 10.5478/MSL.2014.5.3.79

M. Kang, H. R. Cho, Dong Hoon Lee, D. Yeom, Y. Choi, Y. S. Choi

引用次数: 4

In Vitro Inhibitory Effect of Licoricidin on Human Cytochrome P450s 甘草苷对人细胞色素p450的体外抑制作用

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Mass Spectrometry Letters Pub Date : 2014-09-30 DOI: 10.5478/MSL.2014.5.3.84

Sun Ju Kim, O. Heungchan, Jeong Ah Kim, Seung Ho Lee, Sangkyu Lee

{"title":"In Vitro Inhibitory Effect of Licoricidin on Human Cytochrome P450s","authors":"Sun Ju Kim, O. Heungchan, Jeong Ah Kim, Seung Ho Lee, Sangkyu Lee","doi":"10.5478/MSL.2014.5.3.84","DOIUrl":"https://doi.org/10.5478/MSL.2014.5.3.84","url":null,"abstract":"Licoricidin isolated from Glycyrrhiza uralensis is known to have anticancer, anti-nephritic, anti-Helicobacter pylori, and antibacterial effects. In this study, a cocktail probe assay and liquid chromatography-tandem mass spectrometry (LC-MS/MS) were used to investigate the modulating effect of licoricidin on cytochrome P450 (CYP) enzymes in human liver microsomes. When licoricidin was incubated at with CYP probes for 60 min at , it showed potent inhibitory effects on CYP2B6-catalyzed bupropion hydroxylation and CYP2C9-catalyzed diclofenac 4`-hydroxylation with half maximal inhibitory concentration () values of 3.4 and , respectively. The inhibition mode of licoricidin was revealed as competitive, dose-dependent, and non-time-dependent, and following the pattern of Lineweaver-Burk plots. The inhibitory effect of licoricidin has been confirmed in human recombinant cDNA-expressed CYP2B6 and 2C9 with values of 4.5 and , respectively. In conclusion, this study has shown the potent inhibitory effect of licoricidin on CYP2B6 and CYP2C9 activity could be important for predicting potential herb-drug interactions with substrates that mainly undergo CYP2B- and CYP2C9-mediated metabolism.","PeriodicalId":18238,"journal":{"name":"Mass Spectrometry Letters","volume":null,"pages":null},"PeriodicalIF":0.5,"publicationDate":"2014-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87562940","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Investigation on Lipopolysaccharide Activated Microglia by Phosphoproteomics and Phosphoinositide Lipidomics 磷脂蛋白组学和磷脂肌醇组学研究脂多糖活化的小胶质细胞

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Mass Spectrometry Letters Pub Date : 2014-09-30 DOI: 10.5478/MSL.2014.5.3.70

Young Jun Kim, Hackyoung Kim, Kwangmo Noh

{"title":"Investigation on Lipopolysaccharide Activated Microglia by Phosphoproteomics and Phosphoinositide Lipidomics","authors":"Young Jun Kim, Hackyoung Kim, Kwangmo Noh","doi":"10.5478/MSL.2014.5.3.70","DOIUrl":"https://doi.org/10.5478/MSL.2014.5.3.70","url":null,"abstract":"Abstract: Microglia are the confined immune cells of the central nervous system (CNS). In response to injury or infection,microglia readily become activated and release proinflammatory mediators that are believed to contribute to microglia-mediatedneurodegeneration. In the present study, inflammation was induced in the immortalized murine microglial cell line BV-2 bylipopolysaccharide (LPS) treatment. We firstly performed phosphoproteomics analysis and phosphoinositide lipidomics analysiswith LPS activated microglia in order to compare phosphorylation patterns in active and inactive microglia and to detect the pa t-tern of changes in phosphoinositide regulation upon activation of microglia. Mass spectrometry analysis of the phosphopro-teome of the LPS treatment group compared to that of the untreated control group revealed a notable increase in the diversity ofcellular phosphorylation upon LPS treatment. Additionally, a lipidomics analysis detected significant increases in the amounts ofphosphoinositide species in the LPS treatment. This investigation could provide an insight for understanding molecular mecha-nisms underlying microglia-mediated neurodegenerative diseases.Key words: Microglia, Lipopolysaccharide (LPS), Neuroinflammation, Phosphoproteomics, Lipidomics, Phosphoinositide","PeriodicalId":18238,"journal":{"name":"Mass Spectrometry Letters","volume":null,"pages":null},"PeriodicalIF":0.5,"publicationDate":"2014-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82171246","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Simple Carbamidomethylation-Based Isotope Labeling Method for Quantitative Shotgun Proteomics 一种基于碳酰胺甲基化的同位素标记方法用于定量霰弹枪蛋白质组学

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Mass Spectrometry Letters Pub Date : 2014-09-01 DOI: 10.5478/MSL.2014.5.3.63

Donggeun Oh, Sunyoung Lee, Meehyang Kwon, Sook‐Kyung Kim, M. Moon, Dukjin Kang

{"title":"A Simple Carbamidomethylation-Based Isotope Labeling Method for Quantitative Shotgun Proteomics","authors":"Donggeun Oh, Sunyoung Lee, Meehyang Kwon, Sook‐Kyung Kim, M. Moon, Dukjin Kang","doi":"10.5478/MSL.2014.5.3.63","DOIUrl":"https://doi.org/10.5478/MSL.2014.5.3.63","url":null,"abstract":"In this study, we present a new isotope-coded carbamidomethylation (iCCM)-based quantitative proteomics, as a complementary strategy for conventional isotope labeling strategies, with providing the simplicity, ease of use, and robustness. In iCCM-based quantification, two proteome samples can be separately isotope-labeled by means of covalently reaction of all cysteinyl residues in proteins with iodoacetamide (IAA) and its isotope (IAA- 13 C2, D2), denoted as CM and iCCM, respectively, leading to a mass shift of all cysteinyl residues to be + 4 Da. To evaluate iCCM-based isotope labeling in proteomic quantifica- tion, 6 protein standards (i.e., bovine serum albumin, serotransferrin, lysozyme, beta-lactoglobulin, beta-galactosidase, and alpha-lactalbumin) isotopically labeled with IAA and its isotope, mixed equally, and followed by proteolytic digestion. The resulting CM-/iCCM-labeled peptide mixtures were analyzed using a nLC-ESI-FT orbitrap-MS/MS. From our experimental results, we found that the efficiency of iCCM-based quantification is more superior to that of mTRAQ, as a conventional noniso- baric labeling method, in which both of a number of identified peptides from 6 protein standards and the less quantitative varia- tions in the relative abundance ratios of heavy-/light-labeled corresponding peptide pairs. Finally, we applied the developed iCCM-based quantitative method to lung cancer serum proteome in order to evaluate the potential in biomarker discovery study","PeriodicalId":18238,"journal":{"name":"Mass Spectrometry Letters","volume":null,"pages":null},"PeriodicalIF":0.5,"publicationDate":"2014-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86833010","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Simultaneous Determination of Five Porphyrins in Human Urine and Plasma Using High Performance Liquid Chromatography-Tandem Mass Spectrometry 高效液相色谱-串联质谱法同时测定人体尿液和血浆中的5种卟啉类化合物

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Mass Spectrometry Letters Pub Date : 2014-06-30 DOI: 10.5478/MSL.2014.5.2.42

Yeoun Hur, Sookil Tae, Yun-Joo Koh, S. Hong, Y. H. Yoon, H. Jang, Sooji Kim, K. Kim, S. Kang, Young-Shin Lee, S. Han

{"title":"Simultaneous Determination of Five Porphyrins in Human Urine and Plasma Using High Performance Liquid Chromatography-Tandem Mass Spectrometry","authors":"Yeoun Hur, Sookil Tae, Yun-Joo Koh, S. Hong, Y. H. Yoon, H. Jang, Sooji Kim, K. Kim, S. Kang, Young-Shin Lee, S. Han","doi":"10.5478/MSL.2014.5.2.42","DOIUrl":"https://doi.org/10.5478/MSL.2014.5.2.42","url":null,"abstract":"A specific and sensitive liquid chromatography-electrospray ionization tandem mass spectrometry method (LC-ESI- MS/MS) was developed and validated for the simultaneous quantification of porphyrins (coproporphyrin, pentacarboxylporphy- rin, hexacarboxylporphyrin, heptacarboxylporphyrin, and uroporphyrin) in human plasma and urine. Acidified plasma samples and urine samples were prepared by using liquid-liquid extraction using ethyl acetate and protein precipitation with acetonitrile, respectively. The separation was achieved onto a Synergi Fusion RP column (150 mm × 2.0 mm, 4 µm) with a gradient elution of mobile phase A (0.1% formic acid in 2 mmol/L ammonium acetate, v/v) and mobile phase B (20% methanol in acetonitrile, v/ v) at a flow rate of 450 µL/min. Porphyrins and the internal standard (IS), coproporphyrin I- 15 N4, were detected by a tandem mass spectrometer equipped with an electrospray ion source operating in positive ion mode. Multiple reaction monitoring (MRM) transitions of the protonated precursor ions and the related product ions were optimized to increase selectivity and sensi- tivity. The proposed method was validated by assessing selectivity, linearity, limit of quantification (LOQ), precision, accuracy, recovery, and stability. The calibration curves were obtained in the range of 0.1-100 nmol/L and the LOQs were estimated as 0.1 nmol/L for all porphyrins. Results obtained from the validation study of porphyrins showed good accuracy, precision, recov- ery, and stability. Finally, the proposed method was successfully applied to clinical studies on the autism spectrum disorder (ASD) diagnosis of 203 Korean children.","PeriodicalId":18238,"journal":{"name":"Mass Spectrometry Letters","volume":null,"pages":null},"PeriodicalIF":0.5,"publicationDate":"2014-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88110955","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Diagnostic Evaluation of Enzyme Activity Related to Steroid Metabolism by Mass Spectrometry-Based Steroid Profiling 基于质谱的类固醇谱分析对类固醇代谢相关酶活性的诊断评价

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Mass Spectrometry Letters Pub Date : 2014-06-30 DOI: 10.5478/MSL.2014.5.2.35

Man-Ho Choi, B. Chung

引用次数: 1

Optimization of Enzyme Digestion Conditions for Quantification of Glycated Hemoglobin Using Isotope Dilution Liquid Chromatography-Tandem Mass Spectrometry 同位素稀释液相色谱-串联质谱法测定糖化血红蛋白酶切条件的优化

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Mass Spectrometry Letters Pub Date : 2014-06-30 DOI: 10.5478/MSL.2014.5.2.52

Ji-Seon Jeong

{"title":"Optimization of Enzyme Digestion Conditions for Quantification of Glycated Hemoglobin Using Isotope Dilution Liquid Chromatography-Tandem Mass Spectrometry","authors":"Ji-Seon Jeong","doi":"10.5478/MSL.2014.5.2.52","DOIUrl":"https://doi.org/10.5478/MSL.2014.5.2.52","url":null,"abstract":"Glycated hemoglobin (HbA1c) is used as an index of mean glycemia over prolonged periods. This study describes an optimization of enzyme digestion conditions for quantification of non-glycated hemoglobin (HbA0) and HbA1c as diagnostic markers of diabetes mellitus. Both HbA0 and HbA1c were quantitatively determined followed by enzyme digestion using iso- tope dilution liquid chromatography-tandem mass spectrometry (ID-LC-MS/MS) with synthesized N-terminal hexapeptides as standards and synthesized isotope labeled hexapeptides as internal standards. Prior to quantification, each peptide was addition- ally quantified by amino acid composition analysis using ID-LC-MS/MS via acid hydrolysis. Each parameter was considered strictly as a means to improve digestion efficiency and repeatability. Digestion of hemoglobin was optimized when using 100 mM ammonium acetate (pH 4.2) and a Glu-C-to-HbA1c ratio of 1:50 at 37 o C for 20 h. Quantification was satisfactorily reproducible with a 2.6% relative standard deviation. These conditions were recommended for a primary reference method of HbA1c quantification and for the certification of HbA1c reference material.","PeriodicalId":18238,"journal":{"name":"Mass Spectrometry Letters","volume":null,"pages":null},"PeriodicalIF":0.5,"publicationDate":"2014-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84040908","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Tertiary Matrices for the Analysis of Polyethylene Glycols Using MALDI-TOF MS 利用MALDI-TOF质谱分析聚乙二醇的三级基质

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Mass Spectrometry Letters Pub Date : 2014-06-30 DOI: 10.5478/MSL.2014.5.2.49

Jangmi Hong, Taehee Kim, Jeongkwon Kim

引用次数: 3