International Journal of Biology and Life Sciences最新文献

{"title":"Progress in companion diagnosis of colorectal cancer","authors":"Q. Su","doi":"10.54097/ijbls.v2i2.7172","DOIUrl":"https://doi.org/10.54097/ijbls.v2i2.7172","url":null,"abstract":"The incidence of colorectal cancer has been increasing year by year in China in recent years. This article mainly introduces the current common companion diagnostic techniques for colorectal cancer and the monitoring of minimal residual disease and other relevant research progress in the field of colorectal cancer in vitro diagnosis, and compares them with similar detection techniques. The aim is to provide a reference for summarizing the current research status of molecular diagnosis of colorectal cancer.","PeriodicalId":182292,"journal":{"name":"International Journal of Biology and Life Sciences","volume":"54 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122130323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Simultaneous Determination of Zearalenone and Aflatoxin B1 in Maize by High Performance Liquid Chromatography","authors":"Mingjun He, Lipeng Fang, Dawei Li, Yongqi Xiao, Yu Liang, Yang Liu","doi":"10.54097/ijbls.v2i2.7071","DOIUrl":"https://doi.org/10.54097/ijbls.v2i2.7071","url":null,"abstract":"This experiment established a method for the simultaneous detection of Zearalenone (ZEN) and Aflatoxin B1 (AFB1) in corn using High-Performance Liquid Chromatography with fluorescence detector. The sample was extracted with acetonitrile: 0.2% formic acid water (volume ratio: 80:20) and passed through a Prime HLB 3cc purification column. Liquid phase detection conditions: The chromatographic column is Agilent C18 (150mm×4.6mm,5μm) The mobile phase is acetonitrile: water (volume ratio: 80:20). The retention time is qualitative and the external standard method is quantitative. The results showed that the correlation coefficients for ZEN 20～1000ng/mL and AFB1 1～50ng/mL were 0.9989 and 0.9976, respectively, and the detection limits were 5μg/kg and 0.8 μg/kg, with an average spiked recovery of 92.7% to 106.2%. This study combines the detection methods in the current two national standards into one, which can simultaneously achieve rapid and synchronous detection of ZEN and AFB1 in corn. The required extraction and detection time is reduced from 60 minutes to 30 minutes, which is 50% less than the original time. The detection results have high accuracy and good stability.","PeriodicalId":182292,"journal":{"name":"International Journal of Biology and Life Sciences","volume":"8 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131779764","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Study on Immunity-enhancing Function of Golden Globular Immune Protein Factor","authors":"Wen-Jing Bao","doi":"10.54097/ijbls.v2i2.7069","DOIUrl":"https://doi.org/10.54097/ijbls.v2i2.7069","url":null,"abstract":"Golden globular immune protein factor is a mixture of peptides that can enhance personal immunity. The golden globular immune protein factor is mainly composed of cod peptide, milk fat globular membrane, immunoglobulin, lactoferrin and bovine thymosin peptide raw materials. This article mainly reviews the structure and components of the golden globular immune protein factor, and the application of the main components in the middle-aged and elderly people.","PeriodicalId":182292,"journal":{"name":"International Journal of Biology and Life Sciences","volume":"16 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134315051","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficacy of high flexion total knee prosthesis replacement in the treatment of osteoarthrosis of the middle-aged and elderly knee","authors":"Guorui Ding","doi":"10.54097/ijbls.v2i2.7074","DOIUrl":"https://doi.org/10.54097/ijbls.v2i2.7074","url":null,"abstract":"To study the clinical efficacy of high-flexion total knee prosthesis replacement in the treatment of middle-aged and elderly knee osteoarthropathy. Methods: Sixty middle-aged and elderly patients with osteoarthrosis of the knee admitted to the hospital between January 2020 and December 2022 were selected and randomly grouped to compare the various treatment effects of the two groups of patients. Results: The various treatment effects of patients in the observation group were better than those in the control group (P < 0.05). Conclusion: Treatment of middle-aged and elderly patients with knee osteoarthropathy by high-flexion total knee prosthesis can improve the clinical effect and is worth promoting.","PeriodicalId":182292,"journal":{"name":"International Journal of Biology and Life Sciences","volume":"15 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125855075","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of endogenous peptides in maize","authors":"Haoqiang Yin, Yuzheng Li, Jiaqi Zhao, Shanshan Liu, Anran Li, Liuqi Yang, T. Liu, Mingchun Tian, Xinyang Li, Shunxi Wang, Lei Tian","doi":"10.54097/ijbls.v2i2.7070","DOIUrl":"https://doi.org/10.54097/ijbls.v2i2.7070","url":null,"abstract":"The extraction and identification of endogenous peptides from plants are relatively unexplored because of the degradation of peptides after extraction from plant tissues. In this study, we developed an optimized sample preparation protocol that combined constant water heating with plant protease inhibitors and TCA-acetone precipitation, resulting in the effective extraction of endogenous peptide from plants, such as maize, while diminishing unspecific protease activity. The results showed that a total of 2867 endogenous peptides were identified in three maize samples using this method, of which 2119 (73.9%) peptides were commonly identified in all three samples. The length and molecular weight of these peptides ranged from 8 to 25 amino acids and from 729.44 to 2980.62 Da, respectively, with 96.4% of the peptide scores greater than 40. These results indicated that our extraction method is highly reproducible, precise, and wide-ranging, providing optimized information for large-scale identification of plant endogenous peptides.","PeriodicalId":182292,"journal":{"name":"International Journal of Biology and Life Sciences","volume":"50 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115712966","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Application of PROTACs in the Pharmaceutical Direction","authors":"Kechao Zhou, Li Zhang, Yi Liu","doi":"10.54097/ijbls.v2i2.6889","DOIUrl":"https://doi.org/10.54097/ijbls.v2i2.6889","url":null,"abstract":"Proteolysis targeting chimeras (PROTACs) technology is a novel drug development strategy that can treat diseases by selectively degrading targeted proteins. PROTACs consist of two molecules, one of which binds to the target protein while the other binds to the protein degradation enzyme, thus promoting the degradation of the target protein. Compared to the mechanism of action of traditional drugs, PROTAC technology has many advantages, such as high selectivity, reversibility, and low dosage effects. PROTAC technology has been widely applied in multiple fields, especially in cancer research. PROTACs can target cancer-related proteins for degradation, thereby inhibiting tumor growth and metastasis. For instance, PROTACs targeting proteins such as BCL-2 and BRD4 have been extensively researched and applied in cancer treatment. In addition, PROTAC technology can also be applied to multiple fields such as neurodegenerative diseases, immunotherapy, metabolic diseases, and infectious diseases. Although PROTAC technology has broad application prospects, there are still some challenges, such as how to improve the stability and pharmacokinetics of PROTACs, and how to prepare large-scale PROTACs. Therefore, further research and optimization are still needed to further improve PROTAC technology and promote its application in clinical treatment.","PeriodicalId":182292,"journal":{"name":"International Journal of Biology and Life Sciences","volume":"6 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128664937","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pharmaceutical distribution path optimization based on K-nearest neighbor algorithm","authors":"Qiu Yang","doi":"10.54097/ijbls.v2i2.6793","DOIUrl":"https://doi.org/10.54097/ijbls.v2i2.6793","url":null,"abstract":"With the changing situation of the prevention and control of the new crown epidemic, the demand for various types of pharmaceutical supplies is rising, which puts forward very high requirements on the timeliness of distribution. In view of this, this paper establishes a pharmaceutical logistics distribution path optimization model by comprehensively considering constraints such as vehicle loading and latest delivery time in pharmaceutical distribution. In order to solve this type of problem, a nearest neighbor algorithm is proposed, which introduces correlation coefficients to realize the improvement of the performance of the original algorithm. At the end of the paper, the effectiveness of the model and the algorithm is verified by applying a comparative analysis with the existing correlation algorithm with real data from a pharmaceutical company. The experimental results show that the method effectively reduces the delivery mileage and improves the pharmaceutical delivery timeliness.","PeriodicalId":182292,"journal":{"name":"International Journal of Biology and Life Sciences","volume":"16 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115764842","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Zero-Velocity Detection Method Based on Ultrasonic Ranging Assistance","authors":"Juyang Liang, Dasen Luo, Jiawei Hui, Bolong Zhao","doi":"10.54097/ijbls.v2i2.6795","DOIUrl":"https://doi.org/10.54097/ijbls.v2i2.6795","url":null,"abstract":"The accumulated error in inertial pedestrian navigation can be transplanted through the zero-velocity correction algorithm, and the zero-velocity detection algorithm at this stage has the problems of single threshold, easy to miss detection and false detection, large calculation amount and large calculation delay. In order to solve these problems, an ultrasonic ranging auxiliary zero-velocity detection method is proposed. The foot spacing is measured in real time by an ultrasonic sensor placed on both feet, and then a time constraint is added to the Generalized Likelihood Ratio (GLRT) method using the time relationship between the shortest foot spacing and the zero-velocity interval. Experiments show that the accuracy of the proposed algorithm is better than that of the single threshold method under different gait states, and the average accuracy is above 95%. And due to the characteristics of high ultrasonic short-distance accuracy and small size, this method has simple calculation and small delay, which has practical application value.","PeriodicalId":182292,"journal":{"name":"International Journal of Biology and Life Sciences","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128065808","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of microbial transglutaminase on thermal stability of milk protein","authors":"Yun-qing Dai, Hongchen Li","doi":"10.54097/ijbls.v2i2.6545","DOIUrl":"https://doi.org/10.54097/ijbls.v2i2.6545","url":null,"abstract":"At present, Transglutaminase (abbreviated as TG, EC2.3.2.13) has been used in meat products, dairy products and baked goods in Japan and other countries. The stability of MP (Milk protein) colloid system is very important to the quality of dairy products. The effect of MTG (Microbial TG) on the thermal stability of MP was studied in this experiment. The change of HCT (Heat Coagulation Time) of MP emulsion before and after adding MTG was measured. The results show that the effect of MTG on MP leads to the decrease of its solubility in the region of pH < 4.0 or pH > 7.0, but it increases in the range near the isoelectric point of MP (pH 4.0 ~ 6.0). In the range of acidic pH value with low stability, the high calcium ion activity, low Zeta potential and micelle hydration make the milk have poor thermal stability and will solidify rapidly at 150℃. The hydrophobic interaction between MTG and MP changes the conformation of MP, which leads to the change of thermal stability.","PeriodicalId":182292,"journal":{"name":"International Journal of Biology and Life Sciences","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130354595","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"3D Model Construction System for Personalized Rehabilitation Prosthetics Based on Machine Vision","authors":"Yujie Zhang, Kui Cheng, Luyao Zhang","doi":"10.54097/ijbls.v2i2.6140","DOIUrl":"https://doi.org/10.54097/ijbls.v2i2.6140","url":null,"abstract":"At present, the molding process of domestic prosthetic sockets is still mainly made by hand. According to statistics, the number of various types of disabilities in my country has reached 85.02 million, of which more than 6 million are disabled in the lower limbs. Therefore, the installation of prosthetics has become an important means of compensating for missing limbs. , but the old process has many problems and is complicated and backward, heavy, poor performance, long production process cycle and not environmentally friendly, and the appearance is not beautiful. These problems can no longer meet the needs of patients in the current society. At the same time, during the \"13th Five-Year Plan\" period, rehabilitation medicine has become a key industry promoted by the country, and the rehabilitation appliance industry has also developed rapidly. With the promotion of policies and technologies, the market value and space of rehabilitation appliances have shown a good form. Therefore, the research of this project In line with the development trend of the times. This work proposes a personalized rehabilitation prosthetic 3D model construction system based on machine vision. In the era of artificial intelligence, the XTOM 3D scanner under machine vision scans the surface contour of the patient's stump to obtain its point cloud information. The first starting point is innovation, providing a data basis for socket design to improve scanning accuracy and find the most suitable socket structure. Finally, according to the individual needs of the user, the neural network is used to extract video features, analyze the most reasonable movement mode of the patient, and achieve the research on the combination of user behavior and product design, so as to analyze the force of the socket in the later stage. The part of the force analysis is innovated again. By adjusting the gravity distribution of the prosthesis, the angle of force, and the angle of the metal connector, the prosthesis printed by the 3D model for the subsequent industrial-grade 3D printer meets the patient's requirements.","PeriodicalId":182292,"journal":{"name":"International Journal of Biology and Life Sciences","volume":"107 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131622372","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}