International Journal of Biology and Life Sciences最新文献

{"title":"Research on Probiotics and D-allulose","authors":"K. Xu","doi":"10.54097/ijbls.v2i2.6427","DOIUrl":"https://doi.org/10.54097/ijbls.v2i2.6427","url":null,"abstract":"D-allulose has attracted much attention because of its special taste and unique physiological functions, and it can be used as an ideal substitute for sucrose. At present, probiotics have been proven to have a variety of excellent physiological functions and are widely used in various fields.","PeriodicalId":182292,"journal":{"name":"International Journal of Biology and Life Sciences","volume":"747 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133520998","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correlation between serum parathyroid hormone-associated protein concentration and acute ischemic stroke","authors":"Bo Long, Z. Tian, M. Xiong, Yuhan Dong, Youdong Wei","doi":"10.54097/ijbls.v2i2.6143","DOIUrl":"https://doi.org/10.54097/ijbls.v2i2.6143","url":null,"abstract":"Objective To investigate the correlation between serum Parathyroid hormone-related protein (PTHrP) and acute ischemic stroke (AIS). Methods from October 2020 to March 2021, 134 patients with AIS and 34 healthy individuals were enrolled. Serum PTHrP concentration was measured by enzyme-linked immunosorbent assay (ELISA) and subgroup analysis was performed according to Trial of Org 10 172 in acute stroke treatment (TOAST) classification. The severity of AIS was assessed by the National Institutes of Health stroke scale (NIHSS) score and the volume of cerebral infarction was assessed based on head magnetic resonance imaging. The correlation of serum PTHrP concentration with various indicators was analyzed. The 90-day functional prognosis of patients was assessed by the modified Rankin scale. Patients were divided into favorable outcomes group and unfavorable outcomes group and the difference in serum PTHrP concentration between the two groups was compared. Whether serum PTHrP was associated with AIS prognosis was analyzed by Logistic regression.Results The serum PTHrP concentration in AIS group was higher than that in the control group (P=0.000), and the serum PTHrP concentrations of the three common etiological subtypes, LAA, CE and SAO, were higher than those in the control group (all P<0.01). The AUC for distinguishing AIS patients and healthy control by serum PTHrP concentration was 0.751(P<0.0001). The serum PTHrP concentration in the AIS group was associated with the cumulative smoking volume (r=0.201, P=0.021) and APTT (r=-0.188, p=0.034). Serum PTHrP was not associated with TOAST classification (P=0.091), NIHSS score and cerebral infarction volume (all P>0.05), and there was no significant difference in serum PTHrP concentration between the favorable outcomes group and unfavorable outcomes group (P> 0.05). Univariate logistic regression showed that serum PTHrP concentration was not a prognostic factor of AIS [OR=1.000 95% CI (0.999, 1.002) P=0.678]. Conclusion Serum PTHrP concentration was significantly increased in AIS patients. Serum PTHrP might be ancillary for early identification of AIS. Serum PTHrP concentration correlated with cumulative smoking volume and APTT in AIS patients, and could possibly be used to assess the true smoking volume of patients and analyze coagulation function. However, serum PTHrP concentration was not associated with TOAST classification, NIHSS score, cerebral infarction volume and 90-day prognosis, which meant it cannot be used to evaluated the etiology, severity and prognosis of AIS patients.","PeriodicalId":182292,"journal":{"name":"International Journal of Biology and Life Sciences","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127956426","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ophiopogon Japonicus Polysaccharide Plays an Anti-esophageal Cancer Role Through Antioxidant Activity","authors":"Linsong Yang, Meijun Shi, Beiyang Jin, Jia Xu, Yukun Ding, Yucheng Li","doi":"10.54097/ijbls.v2i2.6426","DOIUrl":"https://doi.org/10.54097/ijbls.v2i2.6426","url":null,"abstract":"As a natural product with a variety of biological activities, plant polysaccharide has become a research highlight in the field of antioxidant activity and anti-tumor due to its high efficiency and low toxicity. In this study, the antioxidant and anti-tumor activities of Ophiopogon japonicus polysaccharides (OJP) in vitro and in vivo were investigated. The results showed that OJP had obvious scavenging ability on hydroxyl radical and superoxide radical in a dose-dependent manner. Moreover, the reducing power of OJP also increased and appeared to be dose-dependent. In vivo, it reduced the accumulation of lipofuscin and induced cell apoptosis of Caenorhabditis elegans. In addition, it significantly inhibits the viability of esophageal cancer cell lines Eca109 and TE-1 in vitro and promoted their apoptosis. In conclusion, OJP avoids oxidative damage of organisms and plays an anti-tumor role via its antioxidation. This study aims to promote the in-depth study of plant polysaccharides and provide theoretical reference for the development of antioxidant foods and anti-tumor drugs.","PeriodicalId":182292,"journal":{"name":"International Journal of Biology and Life Sciences","volume":"26 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115138239","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Serum levels of p-tau181 in patients with Parkinson’s disease","authors":"Z. Tian, Long Bo, Xiong Mi, Yuhan Dong, Youdong Wei","doi":"10.54097/ijbls.v2i2.6144","DOIUrl":"https://doi.org/10.54097/ijbls.v2i2.6144","url":null,"abstract":"Background: Parkinson’s disease (PD) is the second most common neurodegenerative disease. Evidence has shown that phosphorylated tau-181 (p-tau181) is involved in the pathological process of PD. The goal of this study was to investigate the changes of serum phosphorylated Microtubule-associated protein tau at threonine-181 in patients with Parkinson's disease and it's correlation with disease severity, cognitive impairment and prognosis. Methods: A total of 40 patients with primary Parkinson's disease who were hospitalized or outpatient in the First Affiliated Hospital of Chongqing Medical University from July 2021 to February 2022 were selected as the study subjects. Patients with secondary Parkinson's disease, Parkinson's syndrome, stroke, Alzheimer's disease, craniocerebral surgery or trauma, severe systemic or infectious diseases, local or systemic infectious diseases, motor neurone disease or other central nervous system diseases were excluded. In addition, 35 healthy subjects with similar age and gender matching were selected as the healthy control group. Age, gender, course of disease, Hoehn-Yahr (H-Y) scale, Unified Parkinson's Disease Scale (UPDRS), and MoCA score were recorded in the Parkinson's disease group. According to the H-Y scale, PD group was divided into PD patients in the advanced stage (H-Y≤2.5, n=16) and PD patients in the advanced stage (H-Y>2.5, n=24). Six months after blood sample collection, we assessed the H-Y rating and UPDRS score in the Parkinson's group again by telephone follow-up. Those with decreased or unchanged H-Y rating or total UPDRS score were divided into good prognosis group (n=25), and those with increased H-Y rating or total UPDRS score were divided into poor prognosis group (n=14). The serum p-tau181 concentration of all subjects was detected and compared by double-antibody sandwich enzyme-linked immunosorbent assay (ELISA), and the correlation between serum p-tau181 level and UPDRS score, MoCA score and prognosis of Parkinson's disease patients was analyzed. Results: Compared with HC, serum p-tau181 concentration in PD patients were higher, but not statistically significant (1.01[0.28-2.63]vs 0.53[0.04-3.72]ug/mL, P=0.55, P>0.05). There was no significant difference in p-tau181 concentration between PD patients in early stage and PD patients in advanced stage (P=0.80 P>0.05), and no significant difference in p-tau181 level between PD patients with cognitive impairment, PD patients with normal cognition and HC patients (P=0.63, p>0.05). P-tau181 was not significantly correlated with disease duration (r=-0.14, P=0.37, P>0.05), UPDRS score (r=0.02, P=0.89, P>0.05), and MoCA score (r= 0.16, P=0.32, P > 0.05). There was no significant difference in serum P-tau181 expression between good prognosis group and poor prognosis group (P=0.74, P > 0.05). Conclusions: Serum expression of p-tau181 increased in PD patients, but no statistical difference was observed, and no clear correlation was found between p-tau18","PeriodicalId":182292,"journal":{"name":"International Journal of Biology and Life Sciences","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130878057","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Heterologous expression of glutamine transaminase in Escherichia coli","authors":"Wenhui Yan, Zengmei Li, Yu Wang","doi":"10.54097/ijbls.v2i2.6142","DOIUrl":"https://doi.org/10.54097/ijbls.v2i2.6142","url":null,"abstract":"Transglutaminase (TGase) has been widely used in food, textile, leather, medicine and other industries. It is currently a research hotspot of industrial production of mTG enzyme to express the gene sequence encoding mTG enzyme in engineering strains by means of genetic engineering. This paper successfully expresses the gene sequence of mTG enzyme in Escherichia coli, providing a reference for industrial production of mTG enzyme.","PeriodicalId":182292,"journal":{"name":"International Journal of Biology and Life Sciences","volume":"95 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128473739","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Status of TG enzyme modified fish gelatin","authors":"Jintao Wu","doi":"10.54097/ijbls.v2i2.6424","DOIUrl":"https://doi.org/10.54097/ijbls.v2i2.6424","url":null,"abstract":"TG enzyme can catalyze the ligation reaction (glycosylation reaction), crosslinking reaction and deamination reaction between or within proteins, and has great application potential in the food field. TG enzyme was used as coagulant to modify fish gelatin, and the effects of crosslinking temperature, pH, TG enzyme dosage, gelatin concentration and crosslinking time on the characteristics of fish gelatin gel were discussed.","PeriodicalId":182292,"journal":{"name":"International Journal of Biology and Life Sciences","volume":"35 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121673236","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Removal of migrated intrauterine device embedded in the urinary bladder wall complicated with cystitis glandularis by a combination of laparoscopy and cystoscopic assistance and literature review","authors":"Jincheng Hua, H. Pan, Shaoping Cheng, H. Deng, Zhen-dong Shi, Jin Dai, Z. Huang, Yu Sang","doi":"10.54097/ijbls.v2i1.5640","DOIUrl":"https://doi.org/10.54097/ijbls.v2i1.5640","url":null,"abstract":"Objective: To explore the mechanism and prevention measures of calculi and adenular cystitis because of IUCD bladder ectopic. Method: Clinical data of one patient with IUCD bladder ectopic cystitis were collected, and the main history, examination data, intraoperative surgical methods and postoperative follow-up were also retrospectively analyzed. Result: The patient recovered well after surgery, without recurrent urinary tract infection after 1 year of follow-up, and recovered from sexual life. Conclusion: IUD allotopic and adenandular cystitis requires complete resection of the lesion, and laparoscopic combination with cystoscopy has obvious results.","PeriodicalId":182292,"journal":{"name":"International Journal of Biology and Life Sciences","volume":"18 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127667109","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Extract natural pigments from Trichoderma","authors":"Hanxin Wang","doi":"10.54097/ijbls.v2i1.5621","DOIUrl":"https://doi.org/10.54097/ijbls.v2i1.5621","url":null,"abstract":"Due to the progress of society, people pay more and more attention to health problems, so more and more people like natural pigments. Therefore, the efficient and safe extraction of natural pigments from microorganisms has become a problem we need to solve. This paper discusses how to extract natural pigments from microorganisms from the perspectives of optimizing media and extraction methods.","PeriodicalId":182292,"journal":{"name":"International Journal of Biology and Life Sciences","volume":"96 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132749317","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Beneficial microorganisms in agriculture","authors":"Guangxu Zhao","doi":"10.54097/ijbls.v2i1.5620","DOIUrl":"https://doi.org/10.54097/ijbls.v2i1.5620","url":null,"abstract":"Beneficial microorganisms are used more and more frequently in the field of agriculture. Beneficial microorganisms can be divided into growth promoting bacteria and biocontrol bacteria. Growth promoting bacteria include improving the nutritional state of plants (such as nitrogen fixing bacteria, phosphorus solubilizing bacteria, potassium solubilizing bacteria, etc.) and improving the state of plants themselves (producing plant hormones, ACC deaminase, ferricarrier, etc.). Beneficial microorganisms are often used as biofertilizers in agricultural production and multifunctional biofertilizers are preferred. In this paper, the classification of beneficial microorganisms and their application in agriculture were reviewed in order to provide reference for the study of beneficial microorganisms.","PeriodicalId":182292,"journal":{"name":"International Journal of Biology and Life Sciences","volume":"105 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125599018","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Progress of research on prodigiosin","authors":"Xin Tan, X. Wang","doi":"10.54097/ijbls.v2i1.5426","DOIUrl":"https://doi.org/10.54097/ijbls.v2i1.5426","url":null,"abstract":"Prodigiosin is a natural red pigment derived primarily from secondary metabolites of microorganisms, especially Serratia marcescens. Prodigiosin has been proven to have antitumor, antibacterial, antimalaria, anti‐insect, antialgae, and immunosuppressive activities, and is gaining increasing important in the global market because of its great potential application value in clinical medicine development, environmental treatment, preparation of food additives, and so on. This paper reviews the progress of the research on prodigiosin.","PeriodicalId":182292,"journal":{"name":"International Journal of Biology and Life Sciences","volume":"9 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132762696","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}