Legume Research最新文献_第5页

Genetic Characterization and Diversity of Rhizobia Isolated from Root Nodules of Green Gram (Vigna radiata L.) found in Central Plateau of India 印度中部高原绿克根瘤中根瘤菌的遗传特征及多样性

IF 0.8 4区生物学

Legume Research Pub Date : 2020-08-24 DOI: 10.18805/LR-4258

M. Stella, Radheshyam Sharma, S. Nema, R. Ramakrishnan, Ashish Kumar

{"title":"Genetic Characterization and Diversity of Rhizobia Isolated from Root Nodules of Green Gram (Vigna radiata L.) found in Central Plateau of India","authors":"M. Stella, Radheshyam Sharma, S. Nema, R. Ramakrishnan, Ashish Kumar","doi":"10.18805/LR-4258","DOIUrl":"https://doi.org/10.18805/LR-4258","url":null,"abstract":"Background: Plant growth promoting rhizobia play an important in agricultural ecosystem through symbiotic association with a wide range of leguminous plants. Legume-rhizobia association is a host specific symbiosis hence the need to identify the strains and the diversity of rhizobia associated with specific type of legume for better bio-prospecting of the associated benefits. A wide range of variation is present among the distribution of rhizobia and greatly affected by geographical locality, edaphic factors and environmental variation with time and space. Molecular techniques have been developed to aid the traditional phenotypic and morpho-cultural techniques in distinguishing the different microbial genera, species and strains. The present study aimed at isolation and morpho-molecular characterization of rhizobia from root nodule of green gram cultivated in central part of India.Methods: In the field-laboratory investigation during 2018-2019, various locations of central India with contrasting agro climatic conditions were surveyed and rhizobia trapping done. A total of 40 rhizobia were retrieved from nodules of green gram and characterized in laboratory based on morphological, biochemical and molecular techniques and results validated for taxonomic identification.Result: In our investigation all forty isolated rhizobia were found phosphate solubilizers, 38 IAA producers and 37 ammonia excretors and grew well at 28oC and 37oC. In carbohydrate fermentation test 34 isolates changed the broth colour from red to yellow with gas formation in durum tubes. Twenty diverse rhizobia isolates were selected with respect to their multifunctional properties and studied for molecular characterization. Based on 16S rRNA gene sequencing, these bacterial strains were identified under two genera: Rhizobium and Brady Rhizobium. Phylogenetic analysis divided 20 rhizobia isolates into two clusters. Major group included 12 strains and minor group included 8 strains.","PeriodicalId":18181,"journal":{"name":"Legume Research","volume":"36 1","pages":"353-361"},"PeriodicalIF":0.8,"publicationDate":"2020-08-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82299894","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Evaluation of Some Morphological Criteria to Drought Tolerance on Seedling of Bambara Groundnut [Vigna subterranea (L.) Verdc.] using Polyethylene Glycol (PEG6000) 板栗(Vigna subterranea, L.)幼苗抗旱性的若干形态学指标评价Verdc。]使用聚乙二醇(PEG6000)

IF 0.8 4区生物学

Legume Research Pub Date : 2020-08-24 DOI: 10.18805/LR-554

Mohamed Milad Draweel, A. Soegianto, L. Soetopo, K. Kuswanto

{"title":"Evaluation of Some Morphological Criteria to Drought Tolerance on Seedling of Bambara Groundnut [Vigna subterranea (L.) Verdc.] using Polyethylene Glycol (PEG6000)","authors":"Mohamed Milad Draweel, A. Soegianto, L. Soetopo, K. Kuswanto","doi":"10.18805/LR-554","DOIUrl":"https://doi.org/10.18805/LR-554","url":null,"abstract":"Background: Bambara groundnut has the ability to drought tolerance and has high nutritional value and is one than Legume crops untapped, which need attention. Therefore in this research, it will look at the relationship between osmotic stress caused by a concentration of PEG and drought tolerance to get some genotypes has ability drought tolerance.Methods: The experiment was conducted in the Tissue culture Laboratory, Department of Agronomy, Faculty of Agriculture, the University of Brawijaya, Malang, Indonesia in January-March 2019. The objective of the experiment was an evaluation of some morphological criteria to drought tolerance on the seedling for thirty genotypes of Bambara groundnut using polyethylene glycol (PEG) 6000 by three concentrations (0%, 5% and 10%). A complete randomized design was used for this experiment with three replicates.Result: The 10% PEG was the most suitable concentration to select Bambara groundnut lines for drought tolerance. Genotypes give different responses to concentrations of PEG. In susceptible lines, there are germination constraints, such as Prevent and germination delayed, the formation of hypocotyl length, root length, fresh weight and dry matter, while drought-tolerant lines can germinate normally. Obtained six lines that were tolerant to drought, five local, namely BBL 1.1, PWBG 5.2.1, PWBG 6, SS 2.4.2, SS 3.4.2 and one from Thailand which is Tvsu 86.","PeriodicalId":18181,"journal":{"name":"Legume Research","volume":"488 1","pages":"175-184"},"PeriodicalIF":0.8,"publicationDate":"2020-08-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72457578","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Genetic Variability and Association Analysis for Various Agro Morphological Traits in Lentil (Lens culinaris M.) 小扁豆(Lens culinaris M.)各种农业形态性状的遗传变异及关联分析

IF 0.8 4区生物学

Legume Research Pub Date : 2020-08-18 DOI: 10.18805/LR-4326

Ashok Kumar, R. K. Gill, Mandeep Singh

{"title":"Genetic Variability and Association Analysis for Various Agro Morphological Traits in Lentil (Lens culinaris M.)","authors":"Ashok Kumar, R. K. Gill, Mandeep Singh","doi":"10.18805/LR-4326","DOIUrl":"https://doi.org/10.18805/LR-4326","url":null,"abstract":"Background: Lentil is an oldest domesticated grain legume in the world. It is cultivated during winter season in India which is a major source of protein (28%) for human consumption. The present study was undertaken to study association analysis of grain yield with other quantitative traits and to estimate direct and indirect effects of various traits on grain yield.Methods: The experiment was carried out using 53 advance breeding lines of lentil in a Randomized Complete Block Design and replicated thrice.Result: The investigation revealed that days to 50% flowering, days to 75% maturity, plant height, pods/plant, biological yield/plant and grain yield/plant have significant differences and wide range of variations during both the year.Pods/plant exhibited high genotypic coefficient of variation, heritability and genetic advance signifying the effects of additive gene effect. Pods/plant and biological yield/plant showed positive and significant correlations with grain yield /plant and also have positive direct effects on grain yield. Therefore, this investigation suggested that pods/plant and biological yield/plant may be focused during selection for genetic gain in lentil.","PeriodicalId":18181,"journal":{"name":"Legume Research","volume":"206 1","pages":"776-779"},"PeriodicalIF":0.8,"publicationDate":"2020-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80424880","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of Imidazolinones Alone and in Combination on Weed Dry Matter Accumulation in Pigeon Pea (Cajanus cajan L.) 咪唑啉酮单用及联用对豇豆杂草干物质积累的影响

IF 0.8 4区生物学

Legume Research Pub Date : 2020-07-15 DOI: 10.18805/LR-4250

Amit Kumar, A. Dhaka, Satish Kumar, Samunder Singh, Ishmadhu

{"title":"Effect of Imidazolinones Alone and in Combination on Weed Dry Matter Accumulation in Pigeon Pea (Cajanus cajan L.)","authors":"Amit Kumar, A. Dhaka, Satish Kumar, Samunder Singh, Ishmadhu","doi":"10.18805/LR-4250","DOIUrl":"https://doi.org/10.18805/LR-4250","url":null,"abstract":"Background: Among pulse crops, pigeon pea [Cajanus cajan (L.) Millsp] is one of the most important pulse crops of India after chick pea. Among various biotic factors limiting pigeon pea yield, weeds are of prime importance which can causes seed yield losses up to 80 per cent. Therefore, weed management is an important key factor for enhancing the productivity of pigeon pea. Imazethapyr, an herbicide of imidazolinones group has been registered for soybean, groundnut and other legumes. Various trials in different regions were conducted to study the effects of imazethapyr alone or in combination for controlling the weeds in different crops but in pigeon pea limited research were conducted. Therefore, current study aimed to study the effect of imidazolinones alone and in combination on weed dry matter accumulation in pigeon pea (Cajanus cajan L.).Methods: Pre and post emergence application of imidazolinones alone and in combination was done and dry matter accumulation by different types of weeds i.e. grassy, broad leaf and sedges in pigeon pea crop were worked out at different interval of crop span.Result: Among all weed controls treatments, two hoeing at 40 and 70 DAS found most effective to control all kind of weeds. Alone application of pendimethalin was not effective to control sedges. PRE application of imidazolinones was comparatively more effective than POE application. Application of imidazolinones in combination with pendimethalin improves its efficacy to control total weeds than alone application of it. Therefore, PRE application of pendimethalin + imazethapyr @ 1000 + 75 g ha-1 proved to be an effective and a profitable alternative to the existing recommendation (two hoeing at 25 and 45 DAS) of weed control in pigeon pea in Haryana locality.","PeriodicalId":18181,"journal":{"name":"Legume Research","volume":"52 1","pages":"826-831"},"PeriodicalIF":0.8,"publicationDate":"2020-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77217009","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Morphological and Ultra-Structural Characterization of Blackgram [Vigna mungo (L.) Hepper] Genotypes Grown under High Temperature Stress 芒豆(Vigna mungo, L.)的形态和超微结构表征高温胁迫下生长的Hepper基因型

IF 0.8 4区生物学

Legume Research Pub Date : 2020-07-15 DOI: 10.18805/LR-4273

C. Partheeban, H. Vijayaraghavan

{"title":"Morphological and Ultra-Structural Characterization of Blackgram [Vigna mungo (L.) Hepper] Genotypes Grown under High Temperature Stress","authors":"C. Partheeban, H. Vijayaraghavan","doi":"10.18805/LR-4273","DOIUrl":"https://doi.org/10.18805/LR-4273","url":null,"abstract":"Background: In this decade, increasing temperatures and adverse environmental factors are expected to influence the crop yield, seed quality and germination ability. Prolonged heat stress affects the pod setting, seed morphology and alters the starch granules.Methods: In this study, Scanning Electron Microscopy (SEM) and Light microscope was used to investigate the variations of morphological and ultra-structural characteristics in blackgram genotypes grown under high temperature (38+2°C) stress (HTS). Nineteen blackgram genotypes were evaluated under open top temperature controlled chamber (OTC) and the genotypes were classified into three categories such as heat tolerant, moderately heat tolerant and heat susceptible genotypes based on the Temperature Induction Response (TIR) and yield reduction percentage under HTS. Two genotypes were selected and examined the seed size, surface pattern, starch granule size and hilum from the each category.Result: Seed coat morphology was captured using SEM and light microscope showed that the tolerant genotype VBG-07-001 had a clear shiny surface, thin and reduced cotyledon fissures, compact surface devoid of surface deposits and pits whereas rest of the genotypes (VBG-06-001, VBN-6, CoBg-11-02, CoBg-11-03 and VBG-08-003) had a rough surface. A bold and well-structured starch granule was observed in heat tolerant lines whereas, unstructured and pleated granules were observed in moderately tolerant and heat stress lines. These morphological and ultra-structural studies revealed remarked diversity among different genotypes that can be regarded as useful to screen the genotypic tolerant characters for HTS.","PeriodicalId":18181,"journal":{"name":"Legume Research","volume":"21 1","pages":"757-763"},"PeriodicalIF":0.8,"publicationDate":"2020-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87483425","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

The Role of the Expansin Gene in the Process of Novel Subterranean Seed Development in Arachis duranensis 膨胀蛋白基因在花生新型地下种子发育过程中的作用

IF 0.8 4区生物学

Legume Research Pub Date : 2020-07-15 DOI: 10.18805/LR-525

Yongli Zhang, Lang Qian, Zhennan Wang, Qingping Zhang, Hui Song

{"title":"The Role of the Expansin Gene in the Process of Novel Subterranean Seed Development in Arachis duranensis","authors":"Yongli Zhang, Lang Qian, Zhennan Wang, Qingping Zhang, Hui Song","doi":"10.18805/LR-525","DOIUrl":"https://doi.org/10.18805/LR-525","url":null,"abstract":"Background: Plants in the genus Arachis produce flowers aerially that develop into gynophores that grow into the ground, where they develop into fruits. Although some aerial gynophores develop into pods aboveground, embryo or seed abortion occurs in these individuals. During pod development, the shell wall initially comprises the majority of the fruit volume, but then the seeds expand up to the total pod volume. Expansins are plant cell wall-loosening proteins involved in cell enlargement and a variety of other developmental processes in which cell-wall modification occurs.Methods: In this study, we analyzed the expansin genes during seed development using RNA-seq data by bioinformatic approaches.Results: We identified five expansin genes exhibited up-regulated expression, while four genes were down-regulated expression using Arachis duranensis RAN-seq data. Multiple transcription factors regulate AdEXPs throughout seed development. Genes co-expressed with AdEXPs are involved in metabolic processes throughout pod development into seed development, reframing the current understanding of the novel subterranean peanut fruits.","PeriodicalId":18181,"journal":{"name":"Legume Research","volume":"277 1","pages":"805-811"},"PeriodicalIF":0.8,"publicationDate":"2020-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72528061","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Reference Genes for Quantitative Real-Time PCR Analysis of Gene Expression in Mung Bean under Abiotic Stress and Cercospora canescens Infection 实时荧光定量PCR分析绿豆在非生物胁迫和黑尾孢感染下基因表达的参考基因

IF 0.8 4区生物学

Legume Research Pub Date : 2020-04-12 DOI: 10.18805/lr-507

X. Ke, L. Yin, J. Xu, W. Sun, X. Xu, Y. Guo, Y. Zuo

引用次数: 1

Enhancing the Water Stress Tolerance in Soybean (Glycine max L.) through Anti-Transpirants and Mulch 通过抗蒸腾剂和地膜提高大豆(Glycine max L.)的抗旱性

IF 0.8 4区生物学

Legume Research Pub Date : 2020-04-12 DOI: 10.18805/lr-4216

P. Revathi, M. Arun, K. Sukumar, S. Dhrua, G. S. M. Bindu

引用次数: 0

Protein Content and Seed Trait Analysis in a Subset of the USDA, ARS, PGRCU Cowpea [Vigna unguiculata (L.) Walp.] Core Collection 美国农业部(USDA)、美国农业部(ARS)、美国农业部(PGRCU)豇豆(Vigna unguiculata, L.)亚群蛋白质含量及种子性状分析Walp。[英语背诵文选

IF 0.8 4区生物学

Legume Research Pub Date : 2020-03-24 DOI: 10.18805/LR-529

J. Morris, B. Tonnis, M. Wang

{"title":"Protein Content and Seed Trait Analysis in a Subset of the USDA, ARS, PGRCU Cowpea [Vigna unguiculata (L.) Walp.] Core Collection","authors":"J. Morris, B. Tonnis, M. Wang","doi":"10.18805/LR-529","DOIUrl":"https://doi.org/10.18805/LR-529","url":null,"abstract":"Cowpea (Vigna unguiculata (L.) Walp is used as a vegetable and feedcrop worldwide, but knowledge is limited regarding the variability and correlations for protein and seed traits within a cowpea core collection. We analyzed protein content and seed trait data from the cowpea core collection representing 111 accessions. The accession PI 354729 produced the significantly highest protein content of 25.527%. Eight Indian cowpea accessions produced significantly high protein content averaging 24.423%. Protein content was significantly correlated with seed pattern (r2 = 0.274***), seed texture (r2 = 0.346***), seed coat color (r2 = 0.136*) and seed pattern significantly correlated with seed texture (r2 = 0.197**). However, protein content showed a significant negative correlation (r2 =-0.333***) with 100 seed weight. One hundred seed weight (gm) showed significantly negative correlations with seed pattern (r2 = -0.191**) and seed texture (r2 = -0.265***). Seed coat color showed a significant negative correlation with seed pattern (r2 = -0.14). Based on these results, potential cowpea cultivars could be developed with improved protein content and morphological seed traits.","PeriodicalId":18181,"journal":{"name":"Legume Research","volume":"249 1","pages":"495-500"},"PeriodicalIF":0.8,"publicationDate":"2020-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83725810","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Light Enrichment, Flowering Asynchrony and Reproduction Success in Two Field-Grown Soybeans in Northern China 中国北方两种大田大豆的光富集、开花不同步及繁殖成功

IF 0.8 4区生物学

Legume Research Pub Date : 2020-03-18 DOI: 10.18805/LR-510

Bing Liu, De-Ning Qu, Jianliang Liu

{"title":"Light Enrichment, Flowering Asynchrony and Reproduction Success in Two Field-Grown Soybeans in Northern China","authors":"Bing Liu, De-Ning Qu, Jianliang Liu","doi":"10.18805/LR-510","DOIUrl":"https://doi.org/10.18805/LR-510","url":null,"abstract":"The flowering process at single plant level in soybean is a continuous dynamic system. Whether a flower can survive to mature depends on where it is located and when it is initiated. A field experiment was conducted to analyse the effect of flowering asynchrony on single flower survival and response of flowers or pods distribution to light enrichment. Our data suggest that early flowers (number 1-5) definitely survive and develop into mature pod because they don’t encounter fierce assimilates competition from other flowers or pods. Later flowers are usually prone to abortion, especially when five or more flowers at the same node in two soybean cultivars (Glycine max L. Merr. namely, Heinong35 and Kennong18). The flowers on sub-raceme frequently fail to develop into pods. Compared with the flowers on main-raceme, that on sub-raceme maybe have weak competitive ability to assimilation. Most flowers on bottom branches successfully develop into pods due to the strong supply of assimilation from the leaves of branches. Light enrichment significantly increase the number of flowers or pods across the main axis. However, the rate of flower abortion is still relatively high by observing the distribution curves of flowers or pods under light enrichment. Soybean plant has the characteristic of excessive flower production and flower abscission is more likely to be expression of biological adaptability.","PeriodicalId":18181,"journal":{"name":"Legume Research","volume":"26 1","pages":"241-246"},"PeriodicalIF":0.8,"publicationDate":"2020-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86099538","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1