实时荧光定量PCR分析绿豆在非生物胁迫和黑尾孢感染下基因表达的参考基因

IF 0.8 4区生物学 Q3 AGRONOMY

Legume Research Pub Date : 2020-04-12 DOI:10.18805/lr-507

X. Ke, L. Yin, J. Xu, W. Sun, X. Xu, Y. Guo, Y. Zuo

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引用次数: 1

摘要

本研究的目的是寻找适合于绿豆(Vigna radiata)实时荧光定量PCR (qPCR)分析的内参基因。结果表明，在干旱和病原菌侵染胁迫下，泛素偶联酶适宜作为内参基因;涝渍条件下伸长因子1-a最稳定;肌动蛋白在生理盐水胁迫下表现最好。这些内参基因通过内涝条件下过氧化氢酶和过氧化物酶的表达谱分析得到进一步证实。本研究结果将有助于提高绿豆基因表达评价的准确性。

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Reference Genes for Quantitative Real-Time PCR Analysis of Gene Expression in Mung Bean under Abiotic Stress and Cercospora canescens Infection

The objective of the study was to identify suitable reference genes that can be used for quantitative real-time PCR (qPCR) analysis in mung bean (Vigna radiata). Therefore, 10 potential reference genes were selected and the results showed that ubiquitin-conjugating enzyme was suitable as reference under drought and pathogen infection stress; elongation factor 1-a was the most stable gene under waterlogging; and actin performed the best under saline stress. These selected reference genes were further confirmed by analysis of the expression profiles of catalase and peroxidase under waterlogging. Our results will contribute to the improvement of the accuracy of gene expression evaluation in mung bean.

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来源期刊

Legume Research AGRONOMY-

CiteScore

1.30

自引率

37.50%

发文量

256

审稿时长

6-12 weeks

期刊介绍： Legume Research An International Journal. The Journal is an official publication of Agricultural Research Communication Centre. It is designed to bring out the original research articles on genetics, breeding, physiology, bacterial avtivity, production, quality, biochemistry and seeds of legumes crops. The objective of the journal is to serve as a forum for scientific community to publish their research findings on legumes ans to provide basis for new research. Journal is being scanned in the important indexing and abstracting services.