Genetic Characterization and Diversity of Rhizobia Isolated from Root Nodules of Green Gram (Vigna radiata L.) found in Central Plateau of India

IF 0.8 4区 生物学 Q3 AGRONOMY
Legume Research Pub Date : 2020-08-24 DOI:10.18805/LR-4258
M. Stella, Radheshyam Sharma, S. Nema, R. Ramakrishnan, Ashish Kumar
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引用次数: 1

Abstract

Background: Plant growth promoting rhizobia play an important in agricultural ecosystem through symbiotic association with a wide range of leguminous plants. Legume-rhizobia association is a host specific symbiosis hence the need to identify the strains and the diversity of rhizobia associated with specific type of legume for better bio-prospecting of the associated benefits. A wide range of variation is present among the distribution of rhizobia and greatly affected by geographical locality, edaphic factors and environmental variation with time and space. Molecular techniques have been developed to aid the traditional phenotypic and morpho-cultural techniques in distinguishing the different microbial genera, species and strains. The present study aimed at isolation and morpho-molecular characterization of rhizobia from root nodule of green gram cultivated in central part of India.Methods: In the field-laboratory investigation during 2018-2019, various locations of central India with contrasting agro climatic conditions were surveyed and rhizobia trapping done. A total of 40 rhizobia were retrieved from nodules of green gram and characterized in laboratory based on morphological, biochemical and molecular techniques and results validated for taxonomic identification.Result: In our investigation all forty isolated rhizobia were found phosphate solubilizers, 38 IAA producers and 37 ammonia excretors and grew well at 28oC and 37oC. In carbohydrate fermentation test 34 isolates changed the broth colour from red to yellow with gas formation in durum tubes. Twenty diverse rhizobia isolates were selected with respect to their multifunctional properties and studied for molecular characterization. Based on 16S rRNA gene sequencing, these bacterial strains were identified under two genera: Rhizobium and Brady Rhizobium. Phylogenetic analysis divided 20 rhizobia isolates into two clusters. Major group included 12 strains and minor group included 8 strains.
印度中部高原绿克根瘤中根瘤菌的遗传特征及多样性
背景:根瘤菌通过与多种豆科植物的共生关系,促进植物生长,在农业生态系统中发挥重要作用。豆科植物与根瘤菌的共生关系是一种宿主特有的共生关系,因此需要对特定类型豆科植物根瘤菌的菌株和多样性进行鉴定,以便更好地进行相关效益的生物勘探。根瘤菌的分布有很大的差异,受地理位置、土壤因素和环境时空变化的影响较大。分子技术已经发展起来,以帮助传统的表型和形态培养技术来区分不同的微生物属、种和菌株。本文对印度中部栽培的绿克根瘤中根瘤菌的分离及形态分子特征进行了研究。方法:在2018-2019年的野外实验室调查中,对印度中部不同农业气候条件的不同地点进行了调查,并进行了根瘤菌捕获。从绿克根瘤中提取了40株根瘤菌,通过形态学、生化和分子技术对其进行了室内鉴定,并对其进行了分类鉴定。结果:40株根瘤菌均为磷酸盐增溶菌,产生IAA菌38株,排出氨菌37株,在28℃和37℃条件下生长良好。在碳水化合物发酵试验中,34株菌株的肉汤颜色由红色变为黄色,并在硬膜管中形成气体。选取了20株不同的根瘤菌,对其多功能特性进行了分子表征。根据16S rRNA基因测序,将这些菌株鉴定为根瘤菌属和布雷迪根瘤菌属。系统发育分析将20株根瘤菌分离株分为两个群。主组12株,次组8株。
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来源期刊
Legume Research
Legume Research AGRONOMY-
CiteScore
1.30
自引率
37.50%
发文量
256
审稿时长
6-12 weeks
期刊介绍: Legume Research An International Journal. The Journal is an official publication of Agricultural Research Communication Centre. It is designed to bring out the original research articles on genetics, breeding, physiology, bacterial avtivity, production, quality, biochemistry and seeds of legumes crops. The objective of the journal is to serve as a forum for scientific community to publish their research findings on legumes ans to provide basis for new research. Journal is being scanned in the important indexing and abstracting services.
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