Malaysian Journal of Microbiology最新文献

{"title":"Effect of Salmonella enterica ser. Typhi on the gut population of Lactobacillus spp. among typhoid patients in Ondo State, Nigeria","authors":"Enabulele Onaiwu, Fadare Olalekan Shadrach","doi":"10.21161/mjm.220165","DOIUrl":"https://doi.org/10.21161/mjm.220165","url":null,"abstract":"Aims: Aberrant gut microbiota has been linked to frequent exposure to enteric pathogens, a condition capable of causing various adverse effects on human health. In this study, we looked into how the typhoid fever condition might affect the lactobacillus population in the human gut. Methodology and results: A total of 424 faecal samples were collected from consented participants, who included 191 patients and 233 apparently healthy individuals. Lactobacilli and Salmonella enterica ser. Typhi ( S . Typhi) was isolated from samples cultured on de Man Rogosa and Sharpe agar (MRS) and xylose lysine deoxycholate agar (XLD), respectively. The overall prevalence of S. Typhi was 104(24.5%), of which 83(43.5%) were patients and 21(9.0%) were healthy controls. Lactobacillus spp. load in patients was significantly lower among the febrile, S . Typhi-positive patients with an average population of 5.5 ± 0.96 log 10 cfu/g compared with the febrile, but S . Typhi-negative patients with 6.41 ± log 10 cfu/g and the apparently healthy respondents with 7.34 ± 1.1 log 10 cfu/g. All the randomly selected S . Typhi strains obtained from both groups of respondents were sensitive to tetracycline and trimethoprim but resistant to chloramphenicol. Resistance to ciprofloxacin (18.2%) and ofloxacin (9.1%) was observed among the strains isolated from the febrile typhoid patients. Conclusion, significance and impact of study: This study has demonstrated an association between the population of Lactobacillus spp. and the presence of S . Typhi in the human gut. In order to ensure the recovery of beneficial bacteria during and after the treatment of infections, it is crucial to promote critical research into new treatment methods.","PeriodicalId":18178,"journal":{"name":"Malaysian Journal of Microbiology","volume":"17 1","pages":""},"PeriodicalIF":0.3,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78257110","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The bactericidal effect of green macroalgae Cladophora sp. from freshwater toward Gram-negative and Gram-positive bacteria","authors":"Amel Saoudi, M. Bouacha, S. Amri, H. Benbouzid, Khedidja Boufligha, S. Belhaoues, Karima Boutarfa, Meriem Abdelkrim, Iness Abdouni, Mourad Bensouilah","doi":"10.21161/mjm.220075","DOIUrl":"https://doi.org/10.21161/mjm.220075","url":null,"abstract":"Aims: The development of new antimicrobial agents towards multidrug-resistant bacteria is one of the most significant challenges facing the healthcare system today. The continuous increase of antimicrobial resistance rates worldwide is a significant threat to public health. Therefore, this study aimed to investigate the antibacterial effect of filamentous macroalgae Cladophora sp. The sample was collected from an Algerian fountainhead of fresh water. A crude hydromethanolic extract (methanol-water) was tested against two standard Gram-negative bacteria: Escherichia coli ATCC25922 and Pseudomonas aeruginosa ATCC27853 , and two standard Gram-positive bacteria: Staphylococcus aureus ATCC25923 and Enterococcus faecalis ATCC 2921. Methodology and results: The antibacterial effect of the hydromethanolic extract of Cladophora sp. was investigated using the well diffusion method to determine the inhibitory diameters and the dilution methods to determine the minimum inhibitory concentration (MIC) and the bactericidal inhibitory concentration (MBC). The results indicated that the hydromethanolic extract of Cladophora sp. is more effective towards Gram-positive bacteria, with a significant effect on S. aureus ATCC 25923, where an inhibitory diameter of 35 mm was recorded. For the Gram-negative, E. coli ATCC25922 was more susceptible with inhibitory diameters of 46 mm, followed by P. aeruginosa ATCC 27853 with 28 mm. The MIC value of hydromethanolic extract of Cladophora sp. was 50 µg/mL for Gram-positive bacteria ( S. aureus ATCC25923 and E. faecalis ATCC29212). However, it was 100 µg/mL for Gram-negative bacteria ( E. coli ATCC25922 and P. aeruginosa ATCC 27853). The best bactericidal effect was observed with Gram-positive with an MBC of 100 µg/mL. The MBC for Gram-negative bacteria was 150 µg/mL. Conclusion, significance and impact of study: The Cladophora sp. macroalgae represent a potential source of bioactive compounds, which could be used in the management and treatment of various microbial infections.","PeriodicalId":18178,"journal":{"name":"Malaysian Journal of Microbiology","volume":"170 1","pages":""},"PeriodicalIF":0.3,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80668081","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Colonization and antagonistic activity of endophytic fungi in seagrasses: Understanding endophyte interaction","authors":"V. Kinamot, Bantoto, Alvin Monotilla, Puspus","doi":"10.21161/mjm.220097","DOIUrl":"https://doi.org/10.21161/mjm.220097","url":null,"abstract":"Endophytic fungal colonization in plants is governed by complex interactions with the defense mechanism of the host and antagonistic effects of other endophytes. In this study, endophytic fungal interaction was assessed by histological examination and co-culture methods. Results showed fungal colonization in the intercellular space of the epidermis and both intercellular and intracellular spaces of the cortical cells suggesting close interaction with their seagrass hosts. Dense colonization, hyphal branching, coiling and formation of networks were observed in the cortical cells. Less competition for space and reliable source of nutrition in the cortex may favor fungal growth. No fungal hyphae were detected in the vascular tissues of seagrasses. All the endophytic fungi isolated from seagrasses showed antagonistic activity. Aspergillus tamarii, A. ochraceopetaliformis, Penicillium citrinum, Beauveria bassiana, Eutypella sp. and Xylaria sp were the most active antagonists. Antagonistic interaction involved deadlock and replacement. Deadlock was associated with physical blocking of the antagonist’s colony by hyphal aggregation and production of inhibitory metabolites. Demarcation line and colony pigmentation in Xylaria sp. during co-culture assay indicated the production of high quantities of inhibitory molecules. Endophytic fungi in seagrasses also produced volatile organic compounds (VOC) which resulted to deadlock at mycelial distance. Thus, endophyte colonization and distribution in seagrass tissues are in�uenced by their interaction with the hosts and other endophytes. But interestingly, cyclical intransitivity of multispecies interaction manifested by these fungal species suggested possible co-existence in seagrass tissues.","PeriodicalId":18178,"journal":{"name":"Malaysian Journal of Microbiology","volume":"55 1","pages":""},"PeriodicalIF":0.3,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88249255","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of bacterial communities in prebiotics and probiotics treated shrimp farms from Kuantan","authors":"Shing Wei Siew, Ira Nadira Kosnin, Nurul Fadzlina Zulkifli, Hajar Fauzan, Normaiza Zamri, Muhammad Fitri Yusof, Ahmad M. Gazali","doi":"10.21161/mjm.220048","DOIUrl":"https://doi.org/10.21161/mjm.220048","url":null,"abstract":"Aims: Prebiotics and probiotics profoundly enhance water quality and shrimp development to tackle infectious disease in shrimp farming. This study evaluated the impact of prebiotics and probiotics treatments in water by assessing the physicochemical properties and bacterial communities in local shrimp ponds. Methodology and results: Water was collected from shrimp pond 1 (SP1), treated with prebiotics and probiotics, and shrimp pond 2 (SP2), treated with only prebiotics. The physicochemical parameters of water from two shrimp ponds were measured, including pH, dissolved oxygen (DO), ammonia concentration and temperature. The total environmental DNA (eDNA) was extracted from the water samples and sequenced using amplicon sequencing targeting the full length of the 16S rRNA gene region via the Oxford Nanopore Technology Flongle. The water quality analysis indicated that SP1 had better water quality than SP2 for shrimp aquaculture. The dominant phyla in both shrimp ponds were Proteobacteria and Bacteroidota . SP1 samples had unique microbiota at the phylum level, including Bdellovibrionota , Firmicutes A , Patescibacteria and unclassified Rhizobiales , Saprospiraceae , Vulcanococcus and HIMB114 at the genus level. The alpha-and beta-diversity showed insignificant differences in microbiota composition between SP1 and SP2 (p-value>0.05). Conclusion, significance and impact of study: Research findings demonstrated that the probiotic-treated shrimp pond (SP1) had better water quality and more diverse microbial communities than the shrimp pond that was not treated with probiotics (SP2).","PeriodicalId":18178,"journal":{"name":"Malaysian Journal of Microbiology","volume":"13 1","pages":""},"PeriodicalIF":0.3,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90242032","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antimicrobial activity of novel Cobalt(II) complexes with Schiff base derived from L-cysteine and 2-substituted benzaldehyde","authors":"M. Pazalja, I. Mahmutović-Dizdarević, Sabina Begić, Alisa Smajović, A. Jerković-Mujkić, M. Avdic, M. Salihović","doi":"10.21161/mjm.220135","DOIUrl":"https://doi.org/10.21161/mjm.220135","url":null,"abstract":"Aims: The aim of this study was to conduct antimicrobial analysis on novel Schiff base-derived cobalt(II) complexes (Co(L1) 2 and Co(L2) 2 )","PeriodicalId":18178,"journal":{"name":"Malaysian Journal of Microbiology","volume":"10 1","pages":""},"PeriodicalIF":0.3,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74314613","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Oncolytic effect of Newcastle disease virus (NDV) strain AF2240 and V4-UPM on hypoxic and normoxic osteosarcoma cells","authors":"Nurneqman Nashreq Kosni, Noraini Abd-Aziz, Abdul Rahman Omar, Saila Ismail, Suet Lin","doi":"10.21161/mjm.230191","DOIUrl":"https://doi.org/10.21161/mjm.230191","url":null,"abstract":"Aims: Hypoxia is believed to be one of the key components contributing to the clinical resistance of cancer therapies. Alternative strategies are under investigation to overcome this resistance and the oncolytic virus stands amongst the others. Newcastle disease virus (NDV) has been demonstrated to possess oncolytic activity against cancer cells. The present study investigated the effects of oncolytic NDV strain AF2240 and V4-UPM on osteosarcoma cells (Saos-2) under normoxic and hypoxic conditions","PeriodicalId":18178,"journal":{"name":"Malaysian Journal of Microbiology","volume":"29 1","pages":""},"PeriodicalIF":0.3,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73302287","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Detection of rpoB gene mutations in clinical isolates of Rifampicin-resistant Mycobacterium tuberculosis in Makassar City, South Sulawesi, Indonesia","authors":"Muttaqin, Z., Massi, M. N., Sjahril, R., Halik, H., Junaedi, M. A., Rifqiani, N., Safitri, N. I., Pratika, M., Islam, I. C., Hamid, F., Natzir, R., Ahmad, A.","doi":"10.21161/mjm.220133","DOIUrl":"https://doi.org/10.21161/mjm.220133","url":null,"abstract":"","PeriodicalId":18178,"journal":{"name":"Malaysian Journal of Microbiology","volume":"88 1","pages":""},"PeriodicalIF":0.3,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82380609","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Viral disease control in strawberry plants: A potential impact of green nanotechnology","authors":"Shafie, R. M., Abdelkader, H. S.","doi":"10.21161/mjm.220063","DOIUrl":"https://doi.org/10.21161/mjm.220063","url":null,"abstract":"","PeriodicalId":18178,"journal":{"name":"Malaysian Journal of Microbiology","volume":"4 1","pages":""},"PeriodicalIF":0.3,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74572105","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A review of current trends of antibacterial Schiff base complexes: Lower and higher transition metal complexes","authors":"Aziza Sarwar, H. Bahron, Bibi Sherino, Anila Ali, Sajjad Bhangwar, Y. Alias","doi":"10.21161/mjm.220103","DOIUrl":"https://doi.org/10.21161/mjm.220103","url":null,"abstract":"The development of metal complexes has inspired researchers to progress in this domain due to their extensive applications in the biological field. Regarding the application, binuclear metal complexes are less explored than their mononuclear counterparts. Recent development in transition metal Schiff base complexes was outlined and presented in detail with their respective vast applications, especially antibacterial. The relationship of their structure, functions, properties","PeriodicalId":18178,"journal":{"name":"Malaysian Journal of Microbiology","volume":"32 1","pages":""},"PeriodicalIF":0.3,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89077569","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of culture conditions on pyocyanin production by recombinant pyocyanin-producing strain Pseudomonas aeruginosa PS39-phzMS","authors":"Nguyen, V. Q., Nguyen, U. H., Nguyen, T. C., Dao, A. T. N., Nguyen, L. T. T.","doi":"10.21161/mjm.220077","DOIUrl":"https://doi.org/10.21161/mjm.220077","url":null,"abstract":"","PeriodicalId":18178,"journal":{"name":"Malaysian Journal of Microbiology","volume":"12 1","pages":""},"PeriodicalIF":0.3,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79260072","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}