The bactericidal effect of green macroalgae Cladophora sp. from freshwater toward Gram-negative and Gram-positive bacteria

IF 0.4 Q4 MICROBIOLOGY

Malaysian Journal of Microbiology Pub Date : 2023-08-01 DOI:10.21161/mjm.220075

Amel Saoudi, M. Bouacha, S. Amri, H. Benbouzid, Khedidja Boufligha, S. Belhaoues, Karima Boutarfa, Meriem Abdelkrim, Iness Abdouni, Mourad Bensouilah

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引用次数: 0

Abstract

Aims: The development of new antimicrobial agents towards multidrug-resistant bacteria is one of the most significant challenges facing the healthcare system today. The continuous increase of antimicrobial resistance rates worldwide is a significant threat to public health. Therefore, this study aimed to investigate the antibacterial effect of filamentous macroalgae Cladophora sp. The sample was collected from an Algerian fountainhead of fresh water. A crude hydromethanolic extract (methanol-water) was tested against two standard Gram-negative bacteria: Escherichia coli ATCC25922 and Pseudomonas aeruginosa ATCC27853 , and two standard Gram-positive bacteria: Staphylococcus aureus ATCC25923 and Enterococcus faecalis ATCC 2921. Methodology and results: The antibacterial effect of the hydromethanolic extract of Cladophora sp. was investigated using the well diffusion method to determine the inhibitory diameters and the dilution methods to determine the minimum inhibitory concentration (MIC) and the bactericidal inhibitory concentration (MBC). The results indicated that the hydromethanolic extract of Cladophora sp. is more effective towards Gram-positive bacteria, with a significant effect on S. aureus ATCC 25923, where an inhibitory diameter of 35 mm was recorded. For the Gram-negative, E. coli ATCC25922 was more susceptible with inhibitory diameters of 46 mm, followed by P. aeruginosa ATCC 27853 with 28 mm. The MIC value of hydromethanolic extract of Cladophora sp. was 50 µg/mL for Gram-positive bacteria ( S. aureus ATCC25923 and E. faecalis ATCC29212). However, it was 100 µg/mL for Gram-negative bacteria ( E. coli ATCC25922 and P. aeruginosa ATCC 27853). The best bactericidal effect was observed with Gram-positive with an MBC of 100 µg/mL. The MBC for Gram-negative bacteria was 150 µg/mL. Conclusion, significance and impact of study: The Cladophora sp. macroalgae represent a potential source of bioactive compounds, which could be used in the management and treatment of various microbial infections.

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淡水大绿藻Cladophora sp.对革兰氏阴性和革兰氏阳性细菌的杀菌作用

目的:针对多药耐药细菌开发新的抗菌药物是当今卫生保健系统面临的最重大挑战之一。世界范围内抗菌素耐药率的持续上升是对公共卫生的重大威胁。因此，本研究旨在探讨丝状大藻Cladophora sp.的抗菌作用。样品采集自阿尔及利亚的一处淡水水源。采用甲醇-水粗提物对2种标准革兰氏阴性菌(大肠杆菌ATCC25922、铜绿假单胞菌ATCC27853)和2种标准革兰氏阳性菌(金黄色葡萄球菌ATCC25923、粪肠球菌ATCC 2921)进行抑菌试验。方法与结果:采用孔扩散法测定其抑菌直径，稀释法测定其最小抑菌浓度(MIC)和杀菌抑菌浓度(MBC)，考察其抑菌效果。结果表明，Cladophora sp. hydrome甲醇提取物对革兰氏阳性菌的抑菌效果更好，对金黄色葡萄球菌ATCC 25923的抑菌效果显著，抑菌直径为35 mm。对于革兰氏阴性菌，大肠杆菌ATCC25922最敏感，抑制直径为46 mm，铜绿假单胞菌ATCC 27853次之，抑制直径为28 mm。革兰氏阳性菌金黄色葡萄球菌(S. aureus) ATCC25923和粪肠球菌(E. faecalis) ATCC29212的MIC值为50µg/mL。而革兰氏阴性菌(大肠杆菌ATCC25922和铜绿假单胞菌atcc27853)为100µg/mL。革兰氏阳性菌的杀菌效果最好，MBC为100µg/mL。革兰氏阴性菌的MBC为150µg/mL。结论、研究意义及影响:Cladophora sp. macroalgae是一种潜在的生物活性化合物来源，可用于各种微生物感染的管理和治疗。

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来源期刊

Malaysian Journal of Microbiology MICROBIOLOGY-

CiteScore

0.70

自引率

0.00%

发文量

审稿时长

11 weeks

期刊介绍： The Malaysian Journal of Microbiology (MJM) publishes high quality microbiology research related to the tropics. These include infectious diseases and antimicrobials. In addition, the journal also publishes research works on the application of microbes for the betterment of human society and the environment. The journal welcomes papers on isolation, identification, characterization and application of microbes and microbial products. The MJM is published under the auspices of the Malaysian Society for Microbiology. It serves as a forum for scientific communication among scientists and academics who deal with microbes and microbial products. The journal publishes research articles, short communications and review articles on various novel aspects of microbiology, which include topics related to medical, pharmaceutical, food, agricultural, industry, plant pathology, biotechnology, microbial genetics, environment, soil, water and biodeterioration. The journal aspires to emphasize the important roles played by microbes in our daily life.