Malaysian Journal of Microbiology最新文献

{"title":"Profiling of coastal silicon solubilizing rhizobacteria with plant-growth promoting properties for enhancing rice seedling early growth performance","authors":"Wan Alia Amiera, Wan Zaidi, Lee Chuen, Ng, Aziz Ahmad","doi":"10.21161/mjm.230277","DOIUrl":"https://doi.org/10.21161/mjm.230277","url":null,"abstract":"Aims: The application of silicon solubilizing rhizobacteria (SSB) with various plant growth-promoting properties to improve the uptake of nutrients by plants is becoming the global trend in sustaining rice production systems. However, the identification and profiling of SSB from the coastal halophyte are still limited. This study was aimed to identify and profile the SSB with plant growth-promoting properties isolated from the coastal halophyte rhizosphere soil. Methodology and results: The rhizosphere soil of halophyte (Poaceae) was sampled and screened for SSB. The SSB obtained was further in vitro screened for lignocellulolytic activities and phosphate solubilization properties. The most potential SSB were clustered and selected using principal component analysis (PCA) and further evaluated for in vitro siderophore and IAA production, followed by rice seed germination and seedling growth performance. A total of 46 SSB were isolated from the coastal halophyte with the silicon solubilizing index ranging 1.01-4.6. Eight SSB isolates (SSB34, SSB37, SSB41, SSB24, SSB45, SSB46, SSB25 and SSB29) were identified and selected from the clustering analysis. Further analysis revealed that rice seedlings inoculated with the isolates SSB24, SSB29 and SSB46 exhibited significant sprouting rates compared with control. All these three SSB isolates were identified using 16S rRNA sequencing as Pantoea stewartii subsp. Indologenes (SSB24), Burkholderia lata strain 383 (SSB29) and Ralstonia syzygii strain ATCC 49543. Conclusion, significance and impact of study: The selected SSB isolates can potentially be suggested as a sustainable approach to promote rice seedling growth. Moreover, exploring SSB under field conditions to improve rice seedling growth through increasing nutrient availability for plant uptake needed to be focused.","PeriodicalId":18178,"journal":{"name":"Malaysian Journal of Microbiology","volume":null,"pages":null},"PeriodicalIF":0.3,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141231339","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synergistic effects of Bacillus salmalaya strain 139SI with fertilizer on nutrient uptake and fertilizer use efficiency of oil palm seedlings","authors":"Md Hoirul Azri, S. Ismail, Rosazlin Abdullah","doi":"10.21161/mjm.230278","DOIUrl":"https://doi.org/10.21161/mjm.230278","url":null,"abstract":"Aims: Plant-microbe interaction in the rhizosphere significantly influences nutrient uptake efficiency. Thus, this research was aimed to investigate the potential of Bacillus salmalaya strain 139SI in increasing nutrient use efficiency through its synergistic effects with fertilizer application. Methodology and results: This research analyzed the effects of B. salmalaya strain 139SI inoculant, fertilizer and a combination of both on soil nutrients, vegetative growth, chlorophyll level, photosynthetic activities, nutrient uptake and nutrient use efficiency in oil palm seedlings for four months in a nursery setting. At the end of the research, the inoculation of B. salmalaya strain 139SI resulted in a significant increase in palm growth, chlorophyll level, photosynthetic activities, nutrient uptake and nutrient use efficiency compared to the untreated group. Soil nutrient analysis demonstrated that the inoculation of B. salmalaya strain 139SI led to a notable increase in available nitrogen within the rhizosphere soil. The findings of this research also indicated a noteworthy synergistic effect between the B. salmalaya strain 139SI inoculant and fertilizer. The most promising outcomes for plant growth performance and nutrient uptake were observed when the B. salmalaya strain 139SI inoculant was added to the fertilized palm. Conclusion, significance and impact of study: This research shows that B. salmalaya strain 139SI may work synergistically with fertilizer to enhance nutrient absorption and increase fertilizer usage efficiency. Integrating B. salmalaya into the nutrient management of oil palm seedlings can potentially reduce reliance on synthetic fertilizers, offering advantages to both farmers and the ecosystem.","PeriodicalId":18178,"journal":{"name":"Malaysian Journal of Microbiology","volume":null,"pages":null},"PeriodicalIF":0.3,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141233071","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Physicochemical properties of Eri pupae flakes and their antibacterial activity against Salmonella sp., Escherichia coli and Staphylococcus aureus","authors":"Y. Endrawati, A. Apriantini, Noviyan Darmawan, Rini Madyastuti Purwono","doi":"10.21161/mjm.220156","DOIUrl":"https://doi.org/10.21161/mjm.220156","url":null,"abstract":"Aims: The objective of this study was to evaluate the physicochemical properties of Eri silkworm ( Samia cynthia ricini ) pupae flour, specifically the pH value, water activity, water content, fat value, ash value and protein value. The study was also aimed to determine the antibacterial activity of Eri silkworm pupae flour against Salmonella sp., Escherichia coli and Staphylococcus aureus . Methodology and results: Pupae meal is a flour made from the extract of the Eri silkworm pupae used as a flour substitute in producing pupae flakes. Pupae meal had two particle size treatments, 60 and 80 mesh, whereas pupae flake had three pupae meal substitution treatments of 0%, 4% and 8%. The physicochemical properties, antioxidant activity and microbiology of pupae meal and flake were analyzed. The results showed that Eri pupae flour met the Indonesia National Standard for Cereals (SNI 01-4270-1996) requirements for food ingredients regarding pH value, water activity, water content, fat value and protein value. The flour also exhibited antibacterial activity against all three bacterial species tested, with S. aureus being the most susceptible. Antioxidant activity and capacity increased as the percentage of pupae meal in the pupae flakes increased. The IC 50 value of pupae meal, 68 µg/mL, was included in the strong category. Salmonella sp. were not found in the pupae meal and flake. Total plate count, E. coli and S. aureus numbers remained within food tolerance limits based on the Indonesia National Standard in both pupae flour and pupae flakes. Conclusion, significance and impact of study: The particle size of pupae flour did not affect its physicochemical properties or antioxidant activity, and microbiologically, it met the Indonesia National Standard for flour. Moreover, The flakes product with pupae flour substitution influenced its physicochemical properties, with the best level at 8% pupae flour for the highest protein content and increased the flakes product's activity and antioxidant capacity. Based on the microbiological analysis of pupa flakes products, The microbial content in the pupa was still within safe limits for human consumption. Research findings suggest that pupae flakes are in accordance with the Indonesia National Standard for Cereal and are a good alternative as a functional food.","PeriodicalId":18178,"journal":{"name":"Malaysian Journal of Microbiology","volume":null,"pages":null},"PeriodicalIF":0.3,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141229935","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Forecasting the impact of global warming on soil bacterial communities using simulated systems","authors":"Grace Lau, Xin Hui, Fan Hui Yin, Mardani Abdul Halim, C. Y. Kqueen, Clemente Michael Wong, Vui Ling","doi":"10.21161/mjm.230323","DOIUrl":"https://doi.org/10.21161/mjm.230323","url":null,"abstract":"Recently, global warming has become more visible, and the entire world is experiencing its effects. Given that global temperature is rising by 0.2 °C (± 0.1 °C) per decade, human-induced warming reached 1 °C above pre-industrial levels around 2017 and is expected to reach 1.5 °C around 2040. However, there is a lack of comprehensive data and long-term monitoring studies on how global warming might impact the diversity of bacteria in terrestrial ecosystems. Since bacteria have a specific range of temperatures for optimal growth and metabolic activity, changes in surrounding temperature may induce a change in soil temperature, leading to alterations in the diversity and composition of soil bacterial communities. Considering the vital ecological functions performed by terrestrial soil bacteria, it is crucial to understand how terrestrial bacteria respond to elevated environmental temperatures. This knowledge will facilitate the development of appropriate intervention strategies to address the anticipated depletion of beneficial bacteria and the potential increase in pathogenic soil bacteria in the upcoming years. This paper explores researchers' efforts over many years to document bacterial diversity and to forecast the impact of global warming on soil bacterial communities using various simulation systems. It also discusses potential mitigation strategies for preserving the pre-warming healthy soil bacteria communities.","PeriodicalId":18178,"journal":{"name":"Malaysian Journal of Microbiology","volume":null,"pages":null},"PeriodicalIF":0.3,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141231297","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Survey of immunological and bacteriological parameters of septicemia in patients hospitalized in ICU departments of Mosul hospitals","authors":"Mahmood Yaseen, Hiyam Adel Altaii","doi":"10.21161/mjm.230254","DOIUrl":"https://doi.org/10.21161/mjm.230254","url":null,"abstract":"Aims: The aim of this study was to identify septicemia in intensive care unit (ICU) patients, determine the antibiotic resistance profiles and minimum inhibitory concentrations (MICs) of all isolates by using the Vitek-2 compact system and study some immunological parameters (IgM, IgG and hs-CRP) in ICU patients, with and without septicemia . Methodology and results: Ninety blood samples from both sexes and different age groups (ranging from 21-90 years) were collected from different hospitals in Mosul city. Twenty-four blood samples were collected from healthy people and used as a control group for the period between July and December of 2022. Samples were subjected to bacterial diagnosis, antibiotic sensitivity test using Vitek2 and measurement of some immunological parameters. The results showed that 24/90 (26.67%) of the blood samples exhibited positive cultures, with Gram-positive bacteria predominating, 14/24 (58.34%), while Gram-negative bacteria represented 10/24 (41.66%) of the positive samples. Antimicrobial susceptibility testing showed that vancomycin, linezolid, imipenem and rifampicin were mostly effective against most Gram-positive septicemia isolates, whereas meropenem, imipenem and colistin were mostly effective against most Gram-negative bacterial isolates. The ELISA results revealed elevated levels of IgM, IgG and hs-CRP in the serum of septicemia patients compared to the healthy control group, with a significant difference in P-value (0.001). In addition, the mean levels of IgM, IgG and hs-CRP in the serum of non-septicemia patients compared to the healthy control group indicated a highly significant difference in P-value (0.001). Conclusion, significance and impact of study: IgM, IgG and hs-CRP levels were significantly different in sepsis patients infected with Gram-positive and Gram-negative bacteria compared to the healthy control group.","PeriodicalId":18178,"journal":{"name":"Malaysian Journal of Microbiology","volume":null,"pages":null},"PeriodicalIF":0.3,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141235345","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of lemon peel essential oil on fecal microbial diversity of tropical Holstein dairy cows","authors":"Novita Dewi Kristanti, Iman Aji, Sri Rahayu, Siska Aditya, Dewi Ratih, Ayu Daning","doi":"10.21161/mjm.230310","DOIUrl":"https://doi.org/10.21161/mjm.230310","url":null,"abstract":"Aims: This study was aimed to determine the effects of adding lemon peel essential oil (EO) as a feed additive to change fecal microbial diversity in tropical Holstein dairy cows. Methodology and results: This study used 12 mid-lactation Friesian Holstein tropical dairy cows with an average milk production of 6.97 ± 0.77 kg/head/day and a body weight of 426.23 ± 11.83 kg, coefficient of variation (CV=6.5%). The doses of lemon peel EO used for each head/day were as follows: S1=0 mL, S2=2.50 mL and S3=5.00 mL. Parameters consisted of an abundance of fecal microbes in phylum and genus levels. Then, the data were analyzed using descriptive quantitative analysis. Lemon peel essential oil with the main component limonene increased the relative population of the Firmicutes phylum at a dose of 5 mL/head/day but decreased the population of the Bacteriodetes phylum. The 2.5 mL/head/day dose has a relatively similar population with controls in Firmicutes and Bacteriodetes phyla. Adding lemon peel essential oil at doses of 2.5 and 5 mL/head/day reduced the population of Prevotellaceae compared to the control. Conclusion, significance and impact of study: This study concluded that a lemon peel EO dose of 5 mL/head/day affects the microbial abundance of Prevotella in the feces of tropical Holstein dairy cows with proteolytic activities. This research impacts increasing milk production due to the inhibition of Prevotella , a proteolytic bacterium.","PeriodicalId":18178,"journal":{"name":"Malaysian Journal of Microbiology","volume":null,"pages":null},"PeriodicalIF":0.3,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141229834","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Detection methods for Legionella pneumophila in diverse environmental conditions: A comparative study of FISH, seminested PCR and conventional culture","authors":"I. Alver, Ayten Kimiran","doi":"10.21161/mjm.230236","DOIUrl":"https://doi.org/10.21161/mjm.230236","url":null,"abstract":"Aims: Legionella bacteria cause Legionnaires' disease and Pontiac fever. It is commonly found in natural water resources and manmade water systems. Environmental conditions such as nutrient deficiency, temperature, pH, disinfectant and the presence of other bacteria can cause Legionella bacteria to pass into the viable but not-culturable (VBNC) phase. This study was aimed to determine appropriate methods to detect Legionella pneumophila bacteria living in water systems with wide temperature and pH ranges threatening human health. Methodology and results: In this study, water samples containing L. pneumophila at a concentration of 10 8 cell/L were exposed to different temperatures (5 °C, 50 °C, 55 °C and 60 °C) and pH (2.2, 5.8, 7.0 and 8.2) values. Conventional culture, FISH and seminested PCR methods were used to detect L. pneumophila . A comparison was made between the methods used in the study to determine the most appropriate method for detecting L. pneumophila bacteria. The results showed that the highest detection rates of L. pneumophila were at 5 °C for 24 h (100%) and at pH 2.2 for 0 th min (100%) by using FISH method. All the samples could be determined by the seminested PCR method. The results of our study showed that the highest detection rates of L. pneumophila were at 5 °C for 24 h (100%) and at pH 2.2 for 0 min (100%) by FISH method. All of the samples could be determined by the seminested PCR method. It was determined that the detection rate was the lowest in the FISH method at 3 min at 60 °C and the highest was 24 h at 5 °C. The lowest detection rate was also observed by using FISH method in the samples exposed to 60 °C for 3 min. Results show that the FISH and seminested PCR methods are the most suitable for detecting L. pneumophila bacteria from water systems exposed to different environmental conditions. Conclusion, significance and impact of study: Different methods (conventional culture, FISH, seminested PCR) used to detect L. pneumophila bacteria were compared in this study. It was concluded that Legionella bacteria passed into the VBNC phase, and compared to molecular methods, the conventional culture method provides a low detection rate of these bacteria. Research findings suggest that it is insufficient to use the conventional culture method alone for the detection of Legionella bacteria from man-made water systems or human samples. This study is important as it is decisive for the determination of the most appropriate method for detecting the human pathogen L. pneumophila bacteria from water samples and the choice for a fast and effective method for the elimination of the infectious agent.","PeriodicalId":18178,"journal":{"name":"Malaysian Journal of Microbiology","volume":null,"pages":null},"PeriodicalIF":0.3,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141229129","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Urinary tract infections due to multi-drug-resistant bacteria at Mohammed VI University Hospital Center of Oujda-Morocco","authors":"Ali Azghar, M. Bensalah, Adnane Naili, Mohammed Lahmer, Imad Mahi, Soufiane Beyyoud, Mohammed Amine Moumna, Hicham Zaidi, E. Benaissa, Y. Lahlou, Mostafa Elouennass, Adil Maleb","doi":"10.21161/mjm.220071","DOIUrl":"https://doi.org/10.21161/mjm.220071","url":null,"abstract":"Aims: Multidrug-resistant bacteria urinary tract infections (MDR-UTI) are a global concern, leading to increased morbidity, mortality and financial costs. The aim of this study was to determine the prevalence of MDR-UTI and establish the epidemiological profile of UTI due to different phenotypes of MDR-UTI. Methodology and results: This is a retrospective study of MDR-UTI assessed at the microbiology laboratory of Mohammed VI University Hospital Center of Oujda over 37 months from March 2016 to April 2019. The study included all requests for cytobacteriological examination of urine (CBEU) according to the instructions of the medical microbiology guidelines. An agar medium was utilized for bacterial culture experimentation. Antibiotic susceptibility testing was performed according to the European Committee on Antimicrobial Susceptibility Testing guidelines. CBEU in UTIs allowed the isolation of 1078 isolates. They were dominated by Gram-negative bacteria (87.65%; n=945), followed by Gram-positive bacteria for 12.34% (n=133). This included 181 (16.80%) multidrug-resistant strains, which were dominated by extended-spectrum beta-lactamase-producing Enterobacteriaceae ( ESBL-E ) (n=144; 79.55%). Young male adults exhibited a higher occurrence of isolated ESBL-E and carbapenem-resistant Acinetobacter baumannii ( CRAB ). As for the distribution by department, there was a near equality between the MDR isolates isolated from outpatients (n= 88; 49%) versus the inpatients (n= 93; 51%). Exactly 152 (84%) isolated MDR cases were observed in patients who did not have a urinary catheter, 157 (87%) and 141 (78%) cases of MDR of UTI were not associated with care and occurred in non-immunocompromised patients, respectively. Prior antibiotic exposure was observed in only 58 (32%) cases. Conclusion, significance and impact of study: This study confirmed the increasing prevalence of multidrug-resistant urinary tract infections in the community and hospitals. The fight against this problem will require all stakeholders' involvement to achieve the goals from one health perspective in line with international agenda.","PeriodicalId":18178,"journal":{"name":"Malaysian Journal of Microbiology","volume":null,"pages":null},"PeriodicalIF":0.3,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141234699","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lignocellulolytic degradation using secondary metabolites of Trichoderma reesei UMK04","authors":"Laila Naher, Mahfuzah Mahmud, Nur Fatihah, Norhafizah, H. Ch’ng, Shafiquzzam Siddic","doi":"10.21161/mjm.220109","DOIUrl":"https://doi.org/10.21161/mjm.220109","url":null,"abstract":"Aims: Fungi species received much attention due to their numerical ability to manufacture various enzymes that can be used to break down cellulose, starch and lipids. However, the conventional way of mycelial incorporation in lignocellulolytic agriculture materials shows less quantity for the degradation of organic compounds. Therefore, fungal metabolites extract received much attention for large amounts of degradation. On the other hand, the effectiveness of fungal metabolite extracts depends on the solvent reaction process. Thus, this study compared degrading organic compounds such as lignin and cellulose in a plate assay experiment using Trichoderma reesei UMK04 secondary metabolites extract using ethyl acetate and hexene solvents. Methodology and results: The T. reesei UMK04 was cultured in potato dextrose broth (PDB) media. Secondary metabolites from the broth culture of T. reseei were extracted using two different solvents of ethyl acetate and hexene separately. The degradation of organic compounds was determined in a plate assay experiment using Jensen media and Tannic acid media. Metabolite extract of T. reesei was used in concentrations of 5 mg/mL, 10 mg/mL, 15 mg/mL and 20 mg/mL. The hollow zone method was used to determine the degradation range. The ethyl acetate solvent extract showed the highest cellulose degradation-like pigmentation formation, which appeared at 6.6 cm on the plate, compared to lignin, which was 3.6 cm. Conclusion, significance and impact of study: The secondary metabolites of T. reesei using ethyl acetate extracts showed higher cellulose degradation, which showed that solvents play the role of more effective compound extraction.","PeriodicalId":18178,"journal":{"name":"Malaysian Journal of Microbiology","volume":null,"pages":null},"PeriodicalIF":0.3,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141228750","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Zinc oxide nanoparticles as a bio elicitor in the growth of Raphanus sativus L. plant tissues","authors":"Rana Tariq","doi":"10.21161/mjm.230214","DOIUrl":"https://doi.org/10.21161/mjm.230214","url":null,"abstract":"Aims: The aim of this study was to investigate the efficiency of zinc oxide nanoparticles at their different concentrations as positive stimuli for the germination of radish seeds and seedling growth by measuring various growth indicators and quantifying their protein content. Methodology and results: Radish seeds were surface sterilized by soaking them in an ethyl alcohol solution, followed by a sodium hypochlorite solution. Seeds were then washed with sterile distilled water and cultured on Murashige and Skoog (MS) solid medium supplemented with 20, 40, 60, 80 and 100 µg/mL of zinc oxide nanoparticles (ZnO-NPs) and compared to control plates. The study examined the positive effect of ZnO-NPs in germinating radish ( Raphanus sativus L.) seeds with healthy seedlings production. The results reflected the role of nanoparticles in increasing cellular components, which were positively remarkable. The effect of treatment with ZnO-NPs showed the enhancement of early germination, in addition to some morphological and physiological changes accompanying seedling growth. Results also revealed that the protein content of seedlings growing on the MS solid medium supplied with 40 µg/mL ZnO-NPs was 1.82 µg/g, much higher than the protein content of seedlings grown on the control medium MS free from nanoparticles. Conclusion, significance and impact of study: Research findings suggest that the ZnO-NPs are able to promote the germination rate of radish seeds","PeriodicalId":18178,"journal":{"name":"Malaysian Journal of Microbiology","volume":null,"pages":null},"PeriodicalIF":0.3,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141229922","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}