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Malaysian Journal of Microbiology最新文献

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Endophytic Colletotrichum siamense from neem leaves: A novel source of antibacterial compounds against Staphylococcus aureus 印楝叶内生炭疽杆菌：抗金黄色葡萄球菌抗菌化合物的新来源
Malaysian Journal of Microbiology Pub Date : 2025-09-08 DOI: 10.21161/mjm.251021
Siti Aisyah Daud, Y. Simone, Latiffah Zakaria, Nurul Farizah Azuddin, Nor Hawani Salikin, Mohd Taufiq Mat Jalil, Syarifah Ab Rashid, Wai-Kit Tung
{"title":"Endophytic Colletotrichum siamense from neem leaves: A novel source of antibacterial compounds against Staphylococcus aureus","authors":"Siti Aisyah Daud, Y. Simone, Latiffah Zakaria, Nurul Farizah Azuddin, Nor Hawani Salikin, Mohd Taufiq Mat Jalil, Syarifah Ab Rashid, Wai-Kit Tung","doi":"10.21161/mjm.251021","DOIUrl":"https://doi.org/10.21161/mjm.251021","url":null,"abstract":"Aims: Multidrug-resistant (MDR) pathogens pose an escalating threat to global health due to their association with hospital-acquired infections and resistance to standard treatments.Hence, this study focused on isolating endophytic fungi from Azadirachta indica (neem) leaves and evaluating their antibacterial potential against MDR bacteria.Methodology and results: An endophytic fungus isolated from neem leaves, identified as Colletotrichum siamense, was subjected to well diffusion, and broth microdilution assays to assess its inhibitory effects.The compounds responsible for its antibacterial activity were further analyzed using gas chromatography-mass spectrometry (GC-MS).The results demonstrated significant inhibition against Staphylococcus aureus and Bacillus cereus, with inhibition zones measuring 18.0 0.6 mm and 12.7 0.6 mm, respectively.The extract exhibited greater potency against S. aureus, with MIC and MBC values of 6.25 mg/mL and 12.5 mg/mL, respectively, compared to B. cereus (MIC = 6.25 mg/mL; MBC = 25 mg/mL).Scanning Electron Microscopy (SEM) of S. aureus cells exposed to the fungal extract revealed cell lysis and structural collapse, further confirming its antibacterial activity.GC-MS analysis of the ethyl acetate fungal extract identified four major compounds: phenylethyl alcohol, indole, 3-methyl-, benzeneacetic acid, and dimethylsulfoxonium formylmethylide. Conclusion, significance and impact of study:The findings highlight neem-derived endophytic fungi as a promising reservoir of antibacterial agents with the potential to combat MDR pathogens.","PeriodicalId":18178,"journal":{"name":"Malaysian Journal of Microbiology","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://mjm.usm.my/uploads/issues/2098/MJM2-25-1021- EV Formatted.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147916929","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
GSK-3β inhibition with antiplasmodial activities from the extracts of actinomycetes isolated from Malaysian forest soil 马来西亚森林土壤中分离出的放线菌提取物的 GSK-3β 抑制作用与抗疟活性
IF 0.3
Malaysian Journal of Microbiology Pub Date : 2023-12-01 DOI: 10.21161/mjm.230011
Mahmud, F., Ali, A. H., Aziz, D. N. A. F. A., Maili, S., Jembun, M., Sudi, S., Dahari, N. D. E., Lai, N. S., How, S. E., Gansau, J. A., Sidek, H. M., Embi, N., Lee, P. C.
{"title":"GSK-3β inhibition with antiplasmodial activities from the extracts of actinomycetes isolated from Malaysian forest soil","authors":"Mahmud, F., Ali, A. H., Aziz, D. N. A. F. A., Maili, S., Jembun, M., Sudi, S., Dahari, N. D. E., Lai, N. S., How, S. E., Gansau, J. A., Sidek, H. M., Embi, N., Lee, P. C.","doi":"10.21161/mjm.230011","DOIUrl":"https://doi.org/10.21161/mjm.230011","url":null,"abstract":"","PeriodicalId":18178,"journal":{"name":"Malaysian Journal of Microbiology","volume":"15 4","pages":""},"PeriodicalIF":0.3,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138988600","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Scaling up the production of myco-coagulant using solid-state fermentation for water treatment 利用固态发酵法扩大生产用于水处理的菌凝剂
IF 0.3
Malaysian Journal of Microbiology Pub Date : 2023-12-01 DOI: 10.21161/mjm.230025
Mamun, A. A., Nedjai, R., Alam, M. Z.
{"title":"Scaling up the production of myco-coagulant using solid-state fermentation for water treatment","authors":"Mamun, A. A., Nedjai, R., Alam, M. Z.","doi":"10.21161/mjm.230025","DOIUrl":"https://doi.org/10.21161/mjm.230025","url":null,"abstract":"","PeriodicalId":18178,"journal":{"name":"Malaysian Journal of Microbiology","volume":"484 ","pages":""},"PeriodicalIF":0.3,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139023259","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Heterologous expression and purification of calmodulin from Plasmodium knowlesi using codon-optimized synthetic gene 利用密码子优化合成基因异源表达和纯化知更鸟疟原虫钙调蛋白
IF 0.3
Malaysian Journal of Microbiology Pub Date : 2023-12-01 DOI: 10.21161/mjm.230020
Robert, A. R., Aladin, S., Budiman, C., Chin, L. P., Kamaruzzaman, K. A., Leow, T. C., Padzil, A. M.
{"title":"Heterologous expression and purification of calmodulin from Plasmodium knowlesi using codon-optimized synthetic gene","authors":"Robert, A. R., Aladin, S., Budiman, C., Chin, L. P., Kamaruzzaman, K. A., Leow, T. C., Padzil, A. M.","doi":"10.21161/mjm.230020","DOIUrl":"https://doi.org/10.21161/mjm.230020","url":null,"abstract":"","PeriodicalId":18178,"journal":{"name":"Malaysian Journal of Microbiology","volume":"39 35","pages":""},"PeriodicalIF":0.3,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139018483","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Bacterial contamination from selected catfish pond farm and its susceptibility to antimicrobial agents 选定鲶鱼池塘养殖场的细菌污染及其对抗菌剂的敏感性
IF 0.3
Malaysian Journal of Microbiology Pub Date : 2023-12-01 DOI: 10.21161/mjm.230018
Zulfakar, S. S., Zarkasi, K. Z., Azman, S. A. A., Shapie, S. A. M., Ariffin, F. D., Bakar, N. F. A.
{"title":"Bacterial contamination from selected catfish pond farm and its susceptibility to antimicrobial agents","authors":"Zulfakar, S. S., Zarkasi, K. Z., Azman, S. A. A., Shapie, S. A. M., Ariffin, F. D., Bakar, N. F. A.","doi":"10.21161/mjm.230018","DOIUrl":"https://doi.org/10.21161/mjm.230018","url":null,"abstract":"","PeriodicalId":18178,"journal":{"name":"Malaysian Journal of Microbiology","volume":"45 59","pages":""},"PeriodicalIF":0.3,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138989040","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Investigation of genetic diversity of Plasmodium knowlesi kelch13-propeller region in Sabah, Malaysia 马来西亚沙巴克雷西疟原虫凯尔奇13-螺旋桨区域遗传多样性调查
IF 0.3
Malaysian Journal of Microbiology Pub Date : 2023-12-01 DOI: 10.21161/mjm.230004
Chee, J. K. L., Chong, E. T. J., Lee, P. C.
{"title":"Investigation of genetic diversity of Plasmodium knowlesi kelch13-propeller region in Sabah, Malaysia","authors":"Chee, J. K. L., Chong, E. T. J., Lee, P. C.","doi":"10.21161/mjm.230004","DOIUrl":"https://doi.org/10.21161/mjm.230004","url":null,"abstract":"","PeriodicalId":18178,"journal":{"name":"Malaysian Journal of Microbiology","volume":"207 ","pages":""},"PeriodicalIF":0.3,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139021359","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Characterization of persistent marine bacterial community profiles isolated from long-term Kappaphycus alvarezii cultures in a closed cultivation system using 16S rDNA analysis 利用 16S rDNA 分析从封闭培养系统中的长期 Kappaphycus alvarezii 培养物中分离的持久性海洋细菌群落特征
IF 0.3
Malaysian Journal of Microbiology Pub Date : 2023-12-01 DOI: 10.21161/mjm.230015
Rupert, R., Rodrigues, K. F., Chong, H. L. H., Yusof, N. A., Yong, W. T. L.
{"title":"Characterization of persistent marine bacterial community profiles isolated from long-term Kappaphycus alvarezii cultures in a closed cultivation system using 16S rDNA analysis","authors":"Rupert, R., Rodrigues, K. F., Chong, H. L. H., Yusof, N. A., Yong, W. T. L.","doi":"10.21161/mjm.230015","DOIUrl":"https://doi.org/10.21161/mjm.230015","url":null,"abstract":"","PeriodicalId":18178,"journal":{"name":"Malaysian Journal of Microbiology","volume":"144 1","pages":""},"PeriodicalIF":0.3,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139012759","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Enterococcal species distribution, antibiotic susceptibility and Van gene frequency among patients at a tertiary hospital in Sabah 沙巴州一家三级医院患者中的肠球菌种类分布、抗生素敏感性和 Van 基因频率
IF 0.3
Malaysian Journal of Microbiology Pub Date : 2023-12-01 DOI: 10.21161/mjm.230007
Mastor, N. N. I., Subbiah, V. K., Bakar, W. N. W. A., Begum, K., Alam, M. J., Hoque, M. Z.
{"title":"Enterococcal species distribution, antibiotic susceptibility and Van gene frequency among patients at a tertiary hospital in Sabah","authors":"Mastor, N. N. I., Subbiah, V. K., Bakar, W. N. W. A., Begum, K., Alam, M. J., Hoque, M. Z.","doi":"10.21161/mjm.230007","DOIUrl":"https://doi.org/10.21161/mjm.230007","url":null,"abstract":"","PeriodicalId":18178,"journal":{"name":"Malaysian Journal of Microbiology","volume":"455 1","pages":""},"PeriodicalIF":0.3,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139013034","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Enhancing stability and activity of β-galactosidase from Kluyveromyces lactis through immobilization on polymethacrylate monolith and optimisation using response surface methodology 通过固定在聚甲基丙烯酸酯整体石上并利用响应面方法进行优化，提高乳酸克鲁瓦酵母β-半乳糖苷酶的稳定性和活性
IF 0.3
Malaysian Journal of Microbiology Pub Date : 2023-12-01 DOI: 10.21161/mjm.230023
Dahari, R. F., Misson, M., Ongkudon, C. M., Saallah, S., Asran, A. M., Dailin, D. J., Chuah, L. F., Enshasy, H. E.
{"title":"Enhancing stability and activity of β-galactosidase from Kluyveromyces lactis through immobilization on polymethacrylate monolith and optimisation using response surface methodology","authors":"Dahari, R. F., Misson, M., Ongkudon, C. M., Saallah, S., Asran, A. M., Dailin, D. J., Chuah, L. F., Enshasy, H. E.","doi":"10.21161/mjm.230023","DOIUrl":"https://doi.org/10.21161/mjm.230023","url":null,"abstract":"","PeriodicalId":18178,"journal":{"name":"Malaysian Journal of Microbiology","volume":"29 4","pages":""},"PeriodicalIF":0.3,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139016454","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The effect of simulated copper stress on genes associated with the metabolism of polyphosphates and polyhydroxyalkanoates in Bacillus thuringiensis Y1 模拟铜胁迫对苏云金芽孢杆菌 Y1 中多磷酸盐和多羟基烷酸代谢相关基因的影响
IF 0.3
Malaysian Journal of Microbiology Pub Date : 2023-12-01 DOI: 10.21161/mjm.230003
Yik, L. Y., Chin, G. J. W. L., Joseph, C. G., Rodrigues, K. F.
{"title":"The effect of simulated copper stress on genes associated with the metabolism of polyphosphates and polyhydroxyalkanoates in Bacillus thuringiensis Y1","authors":"Yik, L. Y., Chin, G. J. W. L., Joseph, C. G., Rodrigues, K. F.","doi":"10.21161/mjm.230003","DOIUrl":"https://doi.org/10.21161/mjm.230003","url":null,"abstract":"","PeriodicalId":18178,"journal":{"name":"Malaysian Journal of Microbiology","volume":"100 12","pages":""},"PeriodicalIF":0.3,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139020868","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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