Limnology and Oceanography: Methods最新文献

Characterizing the response time of unpumped oxygen optodes for profiling applications 表征非泵送氧光电器件的响应时间为分析应用

IF 1.9 3区地球科学

Limnology and Oceanography: Methods Pub Date : 2025-07-26 DOI: 10.1002/lom3.10711

Ellen Park, David Nicholson, Mathieu Dever, Dariia Atamanchuk, Clark Richards

引用次数: 0

Pressurized plankton observatory offers a new window into deep-sea larval behavior 加压浮游生物观测站为研究深海幼虫的行为提供了一个新的窗口

IF 1.9 3区地球科学

Limnology and Oceanography: Methods Pub Date : 2025-07-23 DOI: 10.1002/lom3.10708

Rodrigo Zúñiga Mouret, Stéphane Hourdez, Molly Curran, Michelle H. DiBenedetto, Susan W. Mills, Costantino Vetriani, Shawn M. Arellano, Johanna N. J. Weston, Lauren N. Dykman, Ayinde C. Best, Anthony Pires, Lauren S. Mullineaux

{"title":"Pressurized plankton observatory offers a new window into deep-sea larval behavior","authors":"Rodrigo Zúñiga Mouret, Stéphane Hourdez, Molly Curran, Michelle H. DiBenedetto, Susan W. Mills, Costantino Vetriani, Shawn M. Arellano, Johanna N. J. Weston, Lauren N. Dykman, Ayinde C. Best, Anthony Pires, Lauren S. Mullineaux","doi":"10.1002/lom3.10708","DOIUrl":"10.1002/lom3.10708","url":null,"abstract":"The High-Pressure Plankton Observatory (HiPPO) is designed to quantify motions of zooplankton for behavioral study, including swimming and metabolic responses to environmental perturbations. It builds on prior chamber designs while filling gaps in capability for resolving orientation of small (< 1 mm) plankton, tracking their movements over ecologically relevant spatial scales, and recording in flow-through conditions on a vessel at sea. The HiPPO chamber has a direct light path for silhouette imaging of zooplankton as they move vertically and horizontally across a 3.56 cm diameter viewing area. Seawater forced by a high-performance liquid chromatography pump is exchanged continuously through the chamber, but flushing of zooplankton is prevented by fine mesh at the ports. A high-resolution camera/computer setup enables sustained imaging of plankton motions for quantitative analysis. Application of HiPPO to an investigation of larval behavior of deep-sea hydrothermal vent species revealed swimming behaviors similar to those of shallow-water species, including upward and downward helices, meandering, and short hovers. In conditions with microbial biofilm (a potential settlement cue) on a 2024 expedition, vent larvae unexpectedly swam rapidly upward in tight helices at velocities (0.15 cm s−1) higher than those observed in prior experiments with no biofilm (0.03 cm s−1). Many factors varied between the 2024 and earlier trials, so the difference cannot be attributed with certainty to a cue response. This study describes key new features of HiPPO and demonstrates the system's ability to document novel zooplankton behavior.","PeriodicalId":18145,"journal":{"name":"Limnology and Oceanography: Methods","volume":"23 9","pages":"638-651"},"PeriodicalIF":1.9,"publicationDate":"2025-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://aslopubs.onlinelibrary.wiley.com/doi/epdf/10.1002/lom3.10708","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145111253","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"地球科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Embedding a multichannel ion-sensitive field-effect transistor-pH sensor array in marine sediments: a new approach for continuous in situ pH monitoring 在海洋沉积物中嵌入多通道离子敏感场效应晶体管-pH传感器阵列：一种连续原位pH监测的新方法

IF 1.9 3区地球科学

Limnology and Oceanography: Methods Pub Date : 2025-07-19 DOI: 10.1002/lom3.10709

Yoshita Ogawa, Shoji Yamamoto, Kenta Suzuki, Kazuhiro Morioka, Akihide Hemmi, Hajime Kayanne, Hizuru Nakajima

{"title":"Embedding a multichannel ion-sensitive field-effect transistor-pH sensor array in marine sediments: a new approach for continuous in situ pH monitoring","authors":"Yoshita Ogawa, Shoji Yamamoto, Kenta Suzuki, Kazuhiro Morioka, Akihide Hemmi, Hajime Kayanne, Hizuru Nakajima","doi":"10.1002/lom3.10709","DOIUrl":"10.1002/lom3.10709","url":null,"abstract":"Human activities have significantly increased carbon dioxide emissions, leading to global warming and ocean acidification, which threaten marine ecosystems, including coral reefs with high biodiversity. Coral reef maintenance relies on a balance between calcium carbonate formation and dissolution. Among the processes, sandy sediments, covering vast areas and highly sensitive to ocean acidification, require urgent investigations to elucidate their dissolution mechanisms. However, conventional glass electrodes have limitations in continuous monitoring of the spatiotemporal distribution of pH within sediment. To address this, we developed a multichannel ion-sensitive field-effect transistor (ISFET)-pH sensor array with a tantalum oxide sensing membrane, which was embedded in the sediment to enable high-resolution and continuous pH monitoring. A 24-h pH monitoring experiment was conducted in coral reef sediments to validate the method. The performance of the sensor was evaluated through both laboratory and field observations, and a comparison with a conventional glass electrode confirmed that the ISFET-pH sensor provided stable pH measurements within the uncertainty range of the glass electrode. The developed sensor array is a low-cost and durable automatic measurement system, offering an alternative to conventional glass electrodes, which are expensive and fragile. However, optimizing sputtering conditions, annealing processes, and data processing techniques is necessary to reduce environmental influences and enhance measurement accuracy. The proposed array-based observation method enables the acquisition of high-resolution vertical pH profiles and is expected to contribute to the quantitative evaluation of the chemical role of sandy sediments and the elucidation of carbon cycling in coral reef ecosystems.","PeriodicalId":18145,"journal":{"name":"Limnology and Oceanography: Methods","volume":"23 9","pages":"624-637"},"PeriodicalIF":1.9,"publicationDate":"2025-07-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://aslopubs.onlinelibrary.wiley.com/doi/epdf/10.1002/lom3.10709","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145110979","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"地球科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identifying conditions associated with outliers produced by three different chlorophyll fluorometers: A comparison of instrumentation and development of correction formulae 确定与三种不同叶绿素荧光计产生的异常值相关的条件：仪器的比较和校正公式的发展

IF 1.9 3区地球科学

Limnology and Oceanography: Methods Pub Date : 2025-07-10 DOI: 10.1002/lom3.10705

Emily T. Richardson, Tamara E. C. Kraus, Crystal L. Sturgeon, Katy O'Donnell, Brian A. Bergamaschi

{"title":"Identifying conditions associated with outliers produced by three different chlorophyll fluorometers: A comparison of instrumentation and development of correction formulae","authors":"Emily T. Richardson, Tamara E. C. Kraus, Crystal L. Sturgeon, Katy O'Donnell, Brian A. Bergamaschi","doi":"10.1002/lom3.10705","DOIUrl":"10.1002/lom3.10705","url":null,"abstract":"Measurements of chlorophyll concentration reported by fluorometers (fChl) are used in environmental research and monitoring, as inputs to models, and in the interpretation of remote sensing data. Researchers and managers benefit from understanding how to interpret and ensure the accuracy of fChl data collected by in situ fluorometers. Although fChl values produced by different manufacturers are often in agreement with discrete laboratory-derived Chlorophyll a (Chl a) concentration measurements, there are instances in which results significantly differ. Further, when measuring fChl side by side, different fluorometers may report values that differ significantly from each other, despite passing calibration checks prior to deployment. We compared environmental conditions and phytoplankton species composition associated with instances in which fChl measurements from three different fluorometers (EXO2 Total Algae Smart Sensor, YSI Inc./Xylem Inc., Yellow Springs, Ohio; FluoroProbe III, bbe Moldaenke GmbH, Kiel, Germany; WETStar, Sea-Bird Scientific, Bellevue, Washington) were significantly different from laboratory-derived Chl a concentrations. Results indicated that elevated primary productivity, as indicated by high pH, dissolved oxygen, and the ratio of Chl a to phaeophytin, were correlated with underestimated fChl values recorded by each sensor. After removing outliers, we determined unique correction guidance for each of the three sensors and demonstrated that after applying correction formulae, fChl measurements produced by each sensor became directly comparable.","PeriodicalId":18145,"journal":{"name":"Limnology and Oceanography: Methods","volume":"23 9","pages":"673-687"},"PeriodicalIF":1.9,"publicationDate":"2025-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://aslopubs.onlinelibrary.wiley.com/doi/epdf/10.1002/lom3.10705","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145110758","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"地球科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction to “Underwater Zooplankton Enhancement Light Array (UZELA): A technology solution to enhance zooplankton abundance and coral feeding in bleached and non-bleached corals” 修正“水下浮游动物增强光阵列（UZELA）：一种技术解决方案，以提高浮游动物的丰度和珊瑚在白化和非白化珊瑚中的摄食”

IF 1.9 3区地球科学

Limnology and Oceanography: Methods Pub Date : 2025-07-03 DOI: 10.1002/lom3.10707

引用次数: 0

Thermodynamics and kinetics of the isotopic equilibration of nitrogen gas (N2) in water: Implications for biological N2 fixation experiments 水中氮气（N2）同位素平衡的热力学和动力学：生物固氮实验的意义

IF 1.9 3区地球科学

Limnology and Oceanography: Methods Pub Date : 2025-06-30 DOI: 10.1002/lom3.10700

Nicolas Cassar, Hugo Berthelot, Weiyi Tang

{"title":"Thermodynamics and kinetics of the isotopic equilibration of nitrogen gas (N2) in water: Implications for biological N2 fixation experiments","authors":"Nicolas Cassar, Hugo Berthelot, Weiyi Tang","doi":"10.1002/lom3.10700","DOIUrl":"10.1002/lom3.10700","url":null,"abstract":"Better characterization of biological N2 fixation along with its controlling factors is needed for improved projections of the feedbacks between nitrogen cycling, ecosystems productivity, and climate dynamics. Building on an ongoing community effort to refine estimates of biological N2 fixation, we outline several considerations aimed at improving 15N2 incubation measurements. We first show based on a theoretical analysis that the bias associated with equilibrium isotopic fractionation is within the uncertainty of 15N2 incubation experiments, even under conditions with a large headspace to aqueous ratio, such as in soil or sediment incubations. Second, we empirically determine the effects of temperature and agitation on the equilibration kinetics. Shaking intensity seems to be a dominant control on the kinetics of equilibration. Our results show that nearly complete equilibration of dissolved 15N2 is achieved within 4 min of vigorous shaking at 20°C at atmospheric pressure, but significantly slower at lower temperatures. The equations presented in our study are adaptable to varying 15N2 incubation conditions and other trace gas isotope addition experiments.","PeriodicalId":18145,"journal":{"name":"Limnology and Oceanography: Methods","volume":"23 8","pages":"601-607"},"PeriodicalIF":1.9,"publicationDate":"2025-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144832955","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"地球科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Is acidification of common estuarine macroinvertebrates in stable isotope approaches necessary to analyze aquatic food webs? 用稳定同位素方法分析水生食物网是否需要常见河口大型无脊椎动物的酸化？

IF 1.9 3区地球科学

Limnology and Oceanography: Methods Pub Date : 2025-06-27 DOI: 10.1002/lom3.10706

Elena Hauten, Amami Perera, Peter Grønkjær, Christian Möllmann

{"title":"Is acidification of common estuarine macroinvertebrates in stable isotope approaches necessary to analyze aquatic food webs?","authors":"Elena Hauten, Amami Perera, Peter Grønkjær, Christian Möllmann","doi":"10.1002/lom3.10706","DOIUrl":"10.1002/lom3.10706","url":null,"abstract":"We evaluated the effect of in situ acidification of common estuarine macroinvertebrates from the Elbe estuary on stable isotope ratios, as the non-removal of non-dietary carbon can significantly influence aquatic food web analyses. A 10% HCl solution was used to remove inorganic carbon from crustaceans, which potentially biases the true ratio of assimilated dietary carbon. We detected significant differences in the δ13C values of all investigated crustaceans except for the mysid shrimp Mesopodopsis slabberi after acid treatment. On the contrary, acidification impacts on δ15N were only observed in Gammarus spp. samples. A carbonate proxy was additionally computed to evaluate the necessity of acidification because high values indicate high inorganic carbon in the tissue that may alter true δ13C values. Our results indicate that the necessity of acid treatment of common estuarine macroinvertebrates before stable isotope analysis depends on the species-specific carbonate content. Acid treatment is therefore not required for all species when analyzing aquatic food webs.","PeriodicalId":18145,"journal":{"name":"Limnology and Oceanography: Methods","volume":"23 9","pages":"688-696"},"PeriodicalIF":1.9,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://aslopubs.onlinelibrary.wiley.com/doi/epdf/10.1002/lom3.10706","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145111421","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"地球科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Initial evaluation of high-pressure ceramic nanofiltration for dissolved organic carbon isolation from surface waters across the land-to-ocean continuum 高压陶瓷纳滤从陆地到海洋连续体的地表水中分离溶解有机碳的初步评价

IF 1.9 3区地球科学

Limnology and Oceanography: Methods Pub Date : 2025-06-27 DOI: 10.1002/lom3.10702

Sarah A. Messenger, Kali M. Pate, Cody S. Quiroz, Krista Longnecker, Sean P. Sylva, Collin P. Ward

{"title":"Initial evaluation of high-pressure ceramic nanofiltration for dissolved organic carbon isolation from surface waters across the land-to-ocean continuum","authors":"Sarah A. Messenger, Kali M. Pate, Cody S. Quiroz, Krista Longnecker, Sean P. Sylva, Collin P. Ward","doi":"10.1002/lom3.10702","DOIUrl":"10.1002/lom3.10702","url":null,"abstract":"Marine dissolved organic carbon (DOC) is one of the largest reservoirs of fixed carbon on Earth, and its cycling contributes to ocean productivity and carbon storage. Despite its central role, efforts to characterize DOC reactivity and cycling in aquatic systems have been hampered by low recovery during isolation. The most widely applied recovery methods, solid-phase extraction and ultrafiltration, independently capture less than half of seawater DOC. Here we investigate ceramic nanofiltration as a novel method to isolate DOC from surface waters across the land-to-ocean continuum. A bench-scale prototype system employing a 200 Da pore size ceramic nanomembrane consistently retained > 82% of organic probe molecules (181–376 Da) and > 88% of bulk DOC from diverse surface waters. Salt permeation of the nanomembrane was variable (34–70%) across all surface waters, but highest in seawater (63–70%). Coastal surface seawater was size fractionated using a set of ceramic nanomembranes with pore sizes ranging from 200 to 2000 Da. Radiocarbon analysis of the size fractions revealed that an intermediate size class (i.e., 200–450 Da) is notably older than both smaller and larger size classes and bulk DOC, thereby challenging the size-reactivity continuum paradigm within low molecular weight coastal DOC (i.e., < 2000 Da). Together, these results suggest that ceramic nanofiltration may have the potential to effectively isolate DOC and remove salts, thus enabling new experimental insights into the cycling of DOC. If scaled, this technology could be applied to greatly expand our understanding of the role of DOC as a key intermediate in the ocean carbon cycle.","PeriodicalId":18145,"journal":{"name":"Limnology and Oceanography: Methods","volume":"23 8","pages":"562-577"},"PeriodicalIF":1.9,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://aslopubs.onlinelibrary.wiley.com/doi/epdf/10.1002/lom3.10702","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144833361","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"地球科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A correction model for quenching effects on chlorophyll a fluorescence measurements in lakes 湖泊中叶绿素A荧光测量猝灭效应的校正模型

IF 1.9 3区地球科学

Limnology and Oceanography: Methods Pub Date : 2025-06-25 DOI: 10.1002/lom3.10697

Samantha L. Sharp, Alexander L. Forrest, Derek C. Roberts, Alicia Cortés, S. Geoffrey Schladow

{"title":"A correction model for quenching effects on chlorophyll a fluorescence measurements in lakes","authors":"Samantha L. Sharp, Alexander L. Forrest, Derek C. Roberts, Alicia Cortés, S. Geoffrey Schladow","doi":"10.1002/lom3.10697","DOIUrl":"10.1002/lom3.10697","url":null,"abstract":"Characterizing the vertical structure of phytoplankton biomass is key to understanding the light, nutrient, and mixing dynamics driving lake ecosystems. In situ fluorometry is widely used in limnology to obtain chlorophyll a (Chl a) measurements as proxies for phytoplankton biomass. Unfortunately, daytime fluorometry signals are biased by non-photochemical quenching, limiting the value of these measurements. Phytoplankton utilize this quenching process to dissipate excess light energy as heat, which contaminates daytime fluorometry measurements with reductions in measured Chl a. Despite the ubiquitous impacts of non-photochemical quenching on fluorometer measurements, there is no universal correction method for inland waters. We propose a novel model for correcting non-photochemical quenching impacts in lake systems as a simple exponential function of available light in the water column. This model was developed from data collected from two lakes representing the endmembers in terms of lake productivity and clarity, thus producing a model with possible application to other systems. The study sites are ultraoligotrophic Lake Tahoe, CA-NV, and hypereutrophic Clear Lake, CA. Our proposed non-photochemical quenching correction model demonstrates good performance (R2 = 0.74) when tested on an independent dataset from Lake George, NY. We applied the model to vertical data profiles from Lake Tahoe and Clear Lake to more accurately evaluate the vertical distribution of Chl a in these lakes. The results of this research have wide-reaching benefits by enabling more accurate interpretation and application of Chl a fluorescence measurements in lakes with a range of conditions.","PeriodicalId":18145,"journal":{"name":"Limnology and Oceanography: Methods","volume":"23 8","pages":"543-561"},"PeriodicalIF":1.9,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://aslopubs.onlinelibrary.wiley.com/doi/epdf/10.1002/lom3.10697","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144833051","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"地球科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

On the use of high-density polyethylene bottles for long-term storage of total alkalinity samples 关于使用高密度聚乙烯瓶长期保存总碱度样品

IF 1.9 3区地球科学

Limnology and Oceanography: Methods Pub Date : 2025-06-25 DOI: 10.1002/lom3.10703

Ryan J. Woosley, Daina Neithardt, Jessica A. Bruno, Lou Lahn

{"title":"On the use of high-density polyethylene bottles for long-term storage of total alkalinity samples","authors":"Ryan J. Woosley, Daina Neithardt, Jessica A. Bruno, Lou Lahn","doi":"10.1002/lom3.10703","DOIUrl":"10.1002/lom3.10703","url":null,"abstract":"Total alkalinity (TA) plays an important role in buffering seawater and determining how much anthropogenic carbon dioxide the oceans can absorb and mitigate the rise in atmospheric concentrations. Total alkalinity varies with location, depth, and time making it an important variable needed to quantify and monitor ocean acidification, and potentially for ocean alkalinity enhancement interventions. Currently, best practices are to use expensive high-quality borosilicate glass bottles for collecting and storing these samples. However, unlike other carbon system variables, TA is not affected by gas exchange meaning plastic bottles may be suitable for TA sample storage. Plastic bottles are lighter, cheaper, and less prone to breakage making them easier to handle and ship. Here, we test the suitability of high-density polyethylene (HDPE) for collection and long-term storage of TA samples. In two sets of experiments, it was determined that HDPE is not suitable for long-term storage of TA samples as there were large changes in TA over time and precision of duplicate samples was very poor. We hypothesize that HDPE plastic is slightly porous leading to leaching of alkalinity either into or out of the bottle over time impacting the value of the sample. Use of HDPE bottles for TA samples is not recommended for long term sample storage.","PeriodicalId":18145,"journal":{"name":"Limnology and Oceanography: Methods","volume":"23 8","pages":"594-600"},"PeriodicalIF":1.9,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://aslopubs.onlinelibrary.wiley.com/doi/epdf/10.1002/lom3.10703","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144833050","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"地球科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0