GelCam:通过聚丙烯酰胺凝胶沉淀物捕集器观察下沉的颗粒通量

IF 1.9 3区 地球科学 Q2 LIMNOLOGY
Yixuan Song, Melissa Omand, Colleen A. Durkin, Margaret L. Estapa, Ken O. Buesseler
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引用次数: 0

摘要

沉降颗粒在生物碳泵中起着关键作用。虽然以前的研究已经在几天到几年的时间尺度上分析了颗粒碳通量,但很少有研究能够在更短的时间尺度上同时解决多个深度的通量变化。这项研究使用了一组向上的摄像头,这些摄像头由现成的组件制成,每个售价不到500美元,用于在2018年北太平洋EXPORTS活动期间可视化多个深度的粒子通量。这篇手稿是对GelCam工具的第一次全面描述,GelCam以20分钟的间隔捕获沉降到位于沉积物捕集管底部的聚丙烯酰胺凝胶层中的颗粒的延时图像序列。介绍了管道设计和后处理的方法,以及估算颗粒有机碳总通量的两种代理方法。gelcam从单个粒子图像中获得的通量模型与从同步阱测量中获得的真实数据非常吻合。这种方法有助于解决对可获得的开源工具的需求,以便更广泛地观察和量化全球海洋中偶发粒子通量事件的作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

GelCam: Visualizing sinking particle flux via a polyacrylamide gel-based sediment trap

GelCam: Visualizing sinking particle flux via a polyacrylamide gel-based sediment trap

Sinking particles play a key role in the biological carbon pump. While previous studies have analyzed particulate carbon flux over timescales of days to years, few have been able to resolve flux variability on shorter, hourly scales at multiple depths simultaneously. This study uses an array of upward-facing cameras, built from off-the-shelf components for under $500 each, to visualize particle fluxes at multiple depths during the EXPORTS campaign in 2018 in the North Pacific. This manuscript is the first comprehensive description of this tool, called GelCam, which captures a time-lapse image sequence at 20-min intervals of particles that settle into a polyacrylamide gel layer located at the base of a sediment trap tube. Methods are described for the design and post-processing pipeline, in addition to two proxy methods for estimating the total particulate organic carbon flux. The GelCam-derived fluxes modeled from individual particle images show strong agreement with the ground-truth data obtained from coincident trap measurements. This approach helps address the need for accessible, open-source tools to more broadly observe and quantify the role of episodic particle flux events across the global oceans.

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来源期刊
CiteScore
4.80
自引率
3.70%
发文量
56
审稿时长
3 months
期刊介绍: Limnology and Oceanography: Methods (ISSN 1541-5856) is a companion to ASLO''s top-rated journal Limnology and Oceanography, and articles are held to the same high standards. In order to provide the most rapid publication consistent with high standards, Limnology and Oceanography: Methods appears in electronic format only, and the entire submission and review system is online. Articles are posted as soon as they are accepted and formatted for publication. Limnology and Oceanography: Methods will consider manuscripts whose primary focus is methodological, and that deal with problems in the aquatic sciences. Manuscripts may present new measurement equipment, techniques for analyzing observations or samples, methods for understanding and interpreting information, analyses of metadata to examine the effectiveness of approaches, invited and contributed reviews and syntheses, and techniques for communicating and teaching in the aquatic sciences.
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