Limnology and Oceanography: Methods最新文献_第2页

Real-time quality assessment for Biogeochemical Argo radiometric profiles 生物地球化学Argo辐射剖面的实时质量评价

IF 1.9 3区地球科学

Limnology and Oceanography: Methods Pub Date : 2025-06-21 DOI: 10.1002/lom3.10701

Giovanni La Forgia, Emanuele Organelli

{"title":"Real-time quality assessment for Biogeochemical Argo radiometric profiles","authors":"Giovanni La Forgia, Emanuele Organelli","doi":"10.1002/lom3.10701","DOIUrl":"10.1002/lom3.10701","url":null,"abstract":"An ever-increasing number of Biogeochemical (BGC) Argo floats equipped with radiometric sensors have been deployed across the World Ocean. To date, more than 50,000 vertical profiles from 0 to at least 250 dbar of photosynthetically available radiation and downwelling irradiance at 3, narrow wavelengths have been acquired. For scientific use of radiometric data, corrections for temperature effects and sensor drift are necessary. However, these adjustments are only partially provided in delayed mode, almost a year after acquisition and distribution. This makes automatic, real-time quality control (RT-QC) data processing of BGC-Argo radiometry critically important. Nevertheless, only a range test has been applied to real-time radiometric profiles, so far. By leveraging the full dataset of multispectral radiometric measurements from various BGC-Argo platform types, we have developed a robust RT-QC protocol for processing radiometric data and profiles, aimed at identifying potential sensor malfunctions, particularly those related to temperature effects. Data quality flags are attributed to each data point by considering the expected shape of the radiometric profile associated with the solar elevation during data acquisition. For both daytime and nighttime profiles, the new protocols automatically unveil data potentially dominated by temperature effects. The proposed methodology remains resilient to sensor drift and unstable sea conditions, and it also holds promise for adaptation to data from cutting-edge hyper-spectral sensors mounted on BGC-Argo floats.","PeriodicalId":18145,"journal":{"name":"Limnology and Oceanography: Methods","volume":"23 8","pages":"526-542"},"PeriodicalIF":1.9,"publicationDate":"2025-06-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://aslopubs.onlinelibrary.wiley.com/doi/epdf/10.1002/lom3.10701","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144833351","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"地球科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prediction of red tide outbreaks in inter-connected coastal environments using time-series hyperspectral data and transformer-based graph convolution network 利用时间序列高光谱数据和基于变压器的图卷积网络预测互联海岸环境中赤潮爆发

IF 1.9 3区地球科学

Limnology and Oceanography: Methods Pub Date : 2025-06-19 DOI: 10.1002/lom3.10704

Ming Xie, Ying Li, Zhichen Liu, Tao Gou

{"title":"Prediction of red tide outbreaks in inter-connected coastal environments using time-series hyperspectral data and transformer-based graph convolution network","authors":"Ming Xie, Ying Li, Zhichen Liu, Tao Gou","doi":"10.1002/lom3.10704","DOIUrl":"10.1002/lom3.10704","url":null,"abstract":"The accurate predictions on the red tide outbreaks in coastal regions can reduce their negative impacts on the marine environment and human life. Currently, the red tide prediction is generally accomplished by monitoring some related key factors, which are difficult to obtain on large spatial scales. Combining a transformer encoder with a graph convolution network (GCN), this study proposed an integrated model for red tide prediction that makes comprehensive use of the time-series hyperspectral data obtained through remote sensing methods. The topological graphs are constructed based on the multi-band spectral indices in the interconnected observation points, which are further analyzed using a GCN to obtain the topological features. After that, the temporal features of such topological graphs are extracted based on a transformer encoder, which are used for red tide prediction. The results show that the proposed model achieves reasonable predictions using the input period of 3 d before the date of red tide outbreaks, and the accuracy can reach about 92% with the input period of 5 d. The ablation experiments indicate that both the topological features obtained by the GCN and the temporal features obtained by the transformer encoder play significant roles in the prediction task of red tide outbreaks. The proposed model achieves the red tide prediction in interconnected coastal environments through the fusion of spectral-, topological-, and temporal features, and is expected to provide early alarms on red tide outbreaks for maritime and oceanic agencies.","PeriodicalId":18145,"journal":{"name":"Limnology and Oceanography: Methods","volume":"23 9","pages":"612-623"},"PeriodicalIF":1.9,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145110980","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"地球科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Extending monitoring with sediment archive approaches: Comparison of biomonitoring, metabarcoding, and biomarkers to assess past phytoplankton dynamics 利用沉积物档案方法扩展监测：生物监测、元条形码和生物标记物评估过去浮游植物动态的比较

IF 1.9 3区地球科学

Limnology and Oceanography: Methods Pub Date : 2025-06-17 DOI: 10.1002/lom3.10694

Juliane Romahn, Alexandra Schmidt, Jérôme Kaiser, Damian Baranski, Helge W. Arz, Laura S. Epp, Anke Kremp, Miklós Bálint

{"title":"Extending monitoring with sediment archive approaches: Comparison of biomonitoring, metabarcoding, and biomarkers to assess past phytoplankton dynamics","authors":"Juliane Romahn, Alexandra Schmidt, Jérôme Kaiser, Damian Baranski, Helge W. Arz, Laura S. Epp, Anke Kremp, Miklós Bálint","doi":"10.1002/lom3.10694","DOIUrl":"10.1002/lom3.10694","url":null,"abstract":"Sedimentary archives can provide valuable insights into the study of anthropogenic impacts on marine and limnic ecosystems over centennial and millennial timescales, potentially extending the temporal breadth of observation-based biomonitoring. Sedimentary archives allow for the tracking of biodiversity changes over long time periods, potentially including periods before human-induced changes. However, evaluations of biodiversity reconstructions using sedimentary approaches through comparisons with existing observation-based biomonitoring data are limited. Here we compared sedimentary ancient DNA metabarcoding and several biomarkers with >50 years of phytoplankton biomonitoring data from the Baltic Sea. Our findings indicated that both sedimentary ancient DNA metabarcoding and biomarkers reveal historical trends in phytoplankton communities. Sedimentary ancient DNA data was strongly correlated with biomonitoring data, while biomarkers showed weaker correlations, particularly for dinoflagellates. In addition, the sedimentary ancient DNA data indicated the past prevalence of ecological communities with no present-day analogs, highlighting the challenges of using modern observational data to infer historical biodiversity trends. The study underscores the importance of validating sedimentary approaches against observation-based data and calls for further research to improve the taxonomic resolution of metabarcoding and the specificity of biomarkers. These advancements could significantly enhance our ability to reconstruct historical biodiversity trends and inform future conservation strategies.","PeriodicalId":18145,"journal":{"name":"Limnology and Oceanography: Methods","volume":"23 8","pages":"578-593"},"PeriodicalIF":1.9,"publicationDate":"2025-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://aslopubs.onlinelibrary.wiley.com/doi/epdf/10.1002/lom3.10694","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144833024","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"地球科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An aquatic mesocosm array to regulate spatial and temporal water temperature variability 调节水温时空变化的水生中生态阵列

IF 1.9 3区地球科学

Limnology and Oceanography: Methods Pub Date : 2025-06-09 DOI: 10.1002/lom3.10698

Daniel Gibson, Jay Ewing, Samuel B. Fey

{"title":"An aquatic mesocosm array to regulate spatial and temporal water temperature variability","authors":"Daniel Gibson, Jay Ewing, Samuel B. Fey","doi":"10.1002/lom3.10698","DOIUrl":"10.1002/lom3.10698","url":null,"abstract":"Temporal and spatial variability in temperature are key habitat features that will determine individual, population, and community-level responses to global climate warming. Lakes and ponds exhibit thermal heterogeneity in the form of diel and seasonal fluctuations as well as vertical stratification; however, experimental studies of warming in these ecosystems have largely focused on elevated mean temperatures within thermally homogeneous settings. Thus, new tools are needed to incorporate temperature variability into aquatic mesocosm experiments. We present a design for a mesocosm array to simulate lentic environments with different magnitudes of spatial and temporal temperature variability. Each mesocosm consists of a 77-L plastic container heated by two independent band heaters, where water temperature is regulated via a programmable logic controller that receives feedback from thermocouples. We find that the controller is capable of producing high temperatures (> 35°C) and substantial thermal stratification, with low variability between replicate mesocosms. We generated a vertical temperature gradient up to 12.4°C across 54 cm of water depth and diel fluctuations up to 12.2°C in the surface layer (top 14 cm). We additionally demonstrate the utility of the mesocosm array for other common applications, including temperature ramps and real-time transformations of ambient temperature time series. The control system's ability to simultaneously regulate temporal and spatial thermal variability, while being cost-effective and requiring relatively little technical knowledge to assemble, demonstrates the utility of the array for ecological experiments that seek to investigate the impacts of climate warming on thermally variable aquatic ecosystems.","PeriodicalId":18145,"journal":{"name":"Limnology and Oceanography: Methods","volume":"23 7","pages":"467-481"},"PeriodicalIF":1.9,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/lom3.10698","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144635218","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"地球科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Refining resolution settings for analysis of dissolved organic matter in varied natural environments by Fourier-transform ion cyclotron resonance mass spectrometry 改进傅里叶变换离子回旋共振质谱法分析不同自然环境中溶解有机物的分辨率设置

IF 1.9 3区地球科学

Limnology and Oceanography: Methods Pub Date : 2025-06-06 DOI: 10.1002/lom3.10696

Shuchai Gan, Shiting Huang, Pengran Guo, Ying Wu, Faming Wang, Bin Jiang, Yumei Song, Jiachuan Pan, Zheng Gong

{"title":"Refining resolution settings for analysis of dissolved organic matter in varied natural environments by Fourier-transform ion cyclotron resonance mass spectrometry","authors":"Shuchai Gan, Shiting Huang, Pengran Guo, Ying Wu, Faming Wang, Bin Jiang, Yumei Song, Jiachuan Pan, Zheng Gong","doi":"10.1002/lom3.10696","DOIUrl":"10.1002/lom3.10696","url":null,"abstract":"Dissolved organic matter (DOM) is a major carbon reservoir and exhibits high chemo-diversity and similarity. Fourier-transform ion cyclotron resonance mass spectrometry (FT-ICR MS) enables analysis of DOM due to its ultrahigh resolution at various field strengths. Capturing distinguishing features of DOM is especially challenging for lower resolution instruments. Here, we aim to refine resolution settings for various types of DOM. With a low-field 7 Tesla (T) FT-ICR MS, two strategies for tuning resolution were compared with free induction decay (FID) of 1–4 s: the initial mass to charge (m/z) ratio (A) and data size (B). Peak number rises then falls with data size; 16 M leads to loss of low-mass compounds (< 220 m/z). In further, the comparability of intensity-weighted average parameters was evaluated, revealing that m/z, carbon number, H/C, O/C, aromatic index, and double bond equivalent have a coefficient of variation (CV) of < 3%; in contrast, the average number of heteroatoms—P (45%), N (21%), and S (22%)—shows considerable CV (%) with resolution, varying across samples. Furthermore, the minimum required value of resolution varies across samples, ranging from > 300,000 to > 500,000: it increases from riverine water to porewater, and then to seawater DOM, typically exhibiting abundant CHO, CHOS, and CHOP, respectively. For a 7T FT-ICR MS, we propose tailored FID strategies: a 2-s medium FID (resolution > 300,000) for regular DOM, a shorter FID (~ 1 s) for small metabolites with low initial m/z (~ 50–100), and a longer FID (resolution > 500,000) for heteroatom-enriched DOM.","PeriodicalId":18145,"journal":{"name":"Limnology and Oceanography: Methods","volume":"23 7","pages":"509-521"},"PeriodicalIF":1.9,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144635004","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"地球科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Calibration of the transparency tube for estimating turbidity in estuarine systems 河口系统浊度估算用透明管的校正

IF 1.9 3区地球科学

Limnology and Oceanography: Methods Pub Date : 2025-05-29 DOI: 10.1002/lom3.10695

Makenna Thompson, Erik Smith, James L. Pinckney

{"title":"Calibration of the transparency tube for estimating turbidity in estuarine systems","authors":"Makenna Thompson, Erik Smith, James L. Pinckney","doi":"10.1002/lom3.10695","DOIUrl":"10.1002/lom3.10695","url":null,"abstract":"Turbidity, quantified in turbidity units (nephelometric or formazin), is a common and valid measure of water quality related to transparency. A transparency tube (TT) is an economical tool developed to estimate water clarity as an alternative to the Secchi disk, but it is also frequently used to estimate turbidity. Although the relationship between TT measures and turbidity is well characterized for freshwater river and lake systems, this relationship has not been tested for estuarine waters. The objective of the current study was to empirically determine the TT–turbidity relationship for estuarine waters in coastal South Carolina (SC) and compare these results with the traditional freshwater system conversions. We obtained 107 measurements of TT depth, turbidity, total suspended solids, colored dissolved organic matter absorbance, Secchi depth, and chlorophyll a at 22 estuarine locations in SC over a 1-year period. Linear regressions provide conversion equations that can be applied to SC estuarine waters. The TT–turbidity relationship for estuaries was compared with freshwater systems. Our results suggest that the slope of the relationship differs between systems (−1.11 vs. −1.41), resulting in different turbidity estimates for TT measurements for estuarine vs. inland waters. We propose a combined conversion table incorporating estuarine and freshwater (riverine and lacustrine) systems. A TT–turbidity conversion for coastal SC and similar estuarine waters significantly benefits current water quality programs and citizen science groups by producing more accurate turbidity estimates for screening and routine monitoring efforts.","PeriodicalId":18145,"journal":{"name":"Limnology and Oceanography: Methods","volume":"23 7","pages":"500-508"},"PeriodicalIF":1.9,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/lom3.10695","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144635666","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"地球科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A novel, self-contained benthic chamber design for conducting freshwater ecosystem experiments 一种新颖的、独立的底栖生物室设计，用于进行淡水生态系统实验

IF 1.9 3区地球科学

Limnology and Oceanography: Methods Pub Date : 2025-05-20 DOI: 10.1002/lom3.10692

Jonathan W. Lopez, Matthew B. Lodato, Taylor C. Michael, Lauren M. Morris, Carla L. Atkinson

{"title":"A novel, self-contained benthic chamber design for conducting freshwater ecosystem experiments","authors":"Jonathan W. Lopez, Matthew B. Lodato, Taylor C. Michael, Lauren M. Morris, Carla L. Atkinson","doi":"10.1002/lom3.10692","DOIUrl":"10.1002/lom3.10692","url":null,"abstract":"We present a newly developed design for a self-contained benthic chamber for conducting in situ ecosystem experiments in streams, with a focus on biogeochemical processes such as ecosystem metabolism and nutrient cycling. Our design expands upon smaller, portable chamber designs and is meant to answer questions at larger scales. These new chambers allow for a high level of experimental control in the field and can be used to generate spatially explicit data regarding ecosystem processes and to test mechanistic hypotheses. They are built to be deployed within the stream over periods of weeks to months and to withstand natural hydraulic forces of the benthic zone. First, we describe the materials and steps that are needed to construct these chambers in detail. Then, we report the methods and results of a multi-part, diagnostic field study meant to demonstrate the performance and utility of the design. We quantified solute dynamics using a conservative tracer injection, then we estimated ecosystem metabolism across the study site and performed nutrient additions. We detected asymptotic declines in tracer concentrations, calculated nutrient removal rates, and mapped hotspots of ecosystem metabolism. Flow velocity and water depth imposed limitations, but with appropriate methodological forethought these limitations can be minimized. The capacity of our design to accommodate complex, three-dimensional habitats and macrofauna, along with the capability to generate spatially explicit data, are the main advances we present. These advances provide a novel method whereby motivated users can connect mechanistic hypothesis testing with natural ecological processes through ecosystem-level field experiments.","PeriodicalId":18145,"journal":{"name":"Limnology and Oceanography: Methods","volume":"23 7","pages":"451-466"},"PeriodicalIF":1.9,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144635259","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"地球科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Trace analysis of volatile fatty acids in marine waters using modern high-pressure ion chromatography 现代高压离子色谱法对海水中挥发性脂肪酸的痕量分析

IF 1.9 3区地球科学

Limnology and Oceanography: Methods Pub Date : 2025-05-19 DOI: 10.1002/lom3.10686

Samuel I. Pereira, Bo Emilsson, Eoghan P. Reeves

{"title":"Trace analysis of volatile fatty acids in marine waters using modern high-pressure ion chromatography","authors":"Samuel I. Pereira, Bo Emilsson, Eoghan P. Reeves","doi":"10.1002/lom3.10686","DOIUrl":"10.1002/lom3.10686","url":null,"abstract":"Volatile fatty acids (VFAs) are key intermediates in carbon transformation in marine environments and feature widely in models for a hydrothermal origin of life. Quantifying VFAs in hydrothermal fluids is challenging due to their trace concentrations and the high inorganic ion loads of these matrices. Previous methods often rely on manual sample pre-treatment or complex instrumentation (e.g., mass spectrometry), increasing contamination risks, consumable use, and costs. To circumvent such challenges, we developed a simplified quantification method for trace VFAs in seawater-like matrices using a modern high-pressure ion chromatography (HPIC) system. This approach utilizes single-dimension ion exchange chromatography with conductivity detection alone, and a choice of two analytical column options to separate formate, acetate, propionate, butyrate, valerate, pyruvate, and lactate (measured as ∑anion) from inorganic anions. Modern HPIC systems, in addition to being versatile for other analytes (e.g., cations, nutrients), enable higher peak resolution and increased ion exchange capacity, and our tests show this allows for significantly greater trace VFA sensitivity than previous techniques. With careful sample handling and contamination control, our method achieves better absolute limits of detection for smaller sample requirements (≤ 0.3 mL), statistically determined to be below 10 ppb (~ 0.05 μmol/kg for ∑formate, ~ 0.03 μmol/kg for ∑acetate). Additionally, our study provides detailed insights into limiting VFA contamination sources, as well as their stability in storage. Initial analysis of hydrothermal fluids from the Arctic Mid-Ocean Ridges reveals formate (0.6–7.3 μmol/kg) controlled by metastable CO2–H2–HCOOH equilibrium, and unexpectedly low acetate (2.6–5.8 μmol/kg), likely reflecting competition between thermogenic formation and stability.","PeriodicalId":18145,"journal":{"name":"Limnology and Oceanography: Methods","volume":"23 6","pages":"389-412"},"PeriodicalIF":1.9,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144245021","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"地球科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Monitoring Euplotes species dynamics in mixed experiments using genetic markers for quantitative polymerase chain reaction 利用遗传标记进行定量聚合酶链反应，监测混合实验中的常体物种动态

IF 1.9 3区地球科学

Limnology and Oceanography: Methods Pub Date : 2025-05-10 DOI: 10.1002/lom3.10691

Verena Bamberger, Ralph Tollrian, Linda C. Weiss

{"title":"Monitoring Euplotes species dynamics in mixed experiments using genetic markers for quantitative polymerase chain reaction","authors":"Verena Bamberger, Ralph Tollrian, Linda C. Weiss","doi":"10.1002/lom3.10691","DOIUrl":"10.1002/lom3.10691","url":null,"abstract":"Ciliates are widespread and play a major role in ecosystems as they form an important link between primary producers and higher trophic levels. They have been used as a classic model to study predator–prey interactions of co-evolutionary processes. In our experimental system, predators and prey interact dynamically, with prey exhibiting predator-induced defenses and predators potentially adapting through offensive strategies, both of which influence population dynamics. When analyzing population dynamics in similar ciliate species, individuals must be accurately identified and counted. Morphologically, the genus Euplotes is generally identified by the arrangement of cilia and cirri and the position of the macronucleus and micronucleus. This requires expensive and laborious cell counters and time-consuming staining methods. Furthermore, staining methods are not ideal for determining cell numbers, as individual cells may be lost during staining processes. As ciliates are unicellular organisms, we used DNA quantity to determine the number of individuals. We identified unique sequences of three Euplotes species: Euplotes octocarinatus, Euplotes daidaleos, and Euplotes aediculatus using random amplified polymorphic DNA (RAPD) fingerprinting. Using these sequences, we designed species-specific primers for quantitative polymerase chain reaction and generated corresponding standard curves based on microscopic cell counts. Using this method, we are now able to determine cell counts in unknown samples of different Euplotes species within a single experimental system and monitor population growth rates of one or even several species simultaneously. Additionally, using RAPD fingerprinting enables the identification of unique genetic sequences, allowing differentiation between clones of the same species and facilitating measurement of their population growth rates in mixed experiments.","PeriodicalId":18145,"journal":{"name":"Limnology and Oceanography: Methods","volume":"23 7","pages":"442-450"},"PeriodicalIF":1.9,"publicationDate":"2025-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/lom3.10691","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144635461","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"地球科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Meiofauna investigation and taxonomic identification through imaging—a game of compromise 通过影像调查和分类鉴定——一个妥协的游戏

IF 1.9 3区地球科学

Limnology and Oceanography: Methods Pub Date : 2025-04-29 DOI: 10.1002/lom3.10690

Valentin Foulon, Abdesslam Benzinou, Kamal Nasreddine, Abdul Qayyum, Edwin Daché, Valentine Foulquier, Catherine Borremans, Marine Malloci, Colomban De Vargas, Daniela Zeppilli

{"title":"Meiofauna investigation and taxonomic identification through imaging—a game of compromise","authors":"Valentin Foulon, Abdesslam Benzinou, Kamal Nasreddine, Abdul Qayyum, Edwin Daché, Valentine Foulquier, Catherine Borremans, Marine Malloci, Colomban De Vargas, Daniela Zeppilli","doi":"10.1002/lom3.10690","DOIUrl":"10.1002/lom3.10690","url":null,"abstract":"Imaging methods have developed rapidly in recent decades, opening new opportunities for taxonomy and biodiversity studies of marine organisms. In particular, the microscopic size range, which used to be challenging to study due to time-consuming preparation and observation steps, now benefits from high-throughput quantitative imaging methods and the development of fast high-resolution microscopy approaches. Meiofauna, interstitial sediment animals ranging from 20 μm to 1 mm, are important components of ecosystems. These organisms can serve as bioindicators, and the group as a whole holds immense potential for the discovery of new species. However, protocols for studying meiobenthos are highly time-consuming, which helps explain why this group is understudied. We tested five imaging techniques, ranging from low to high resolution, that could accelerate hard-bodied meiofauna studies, both for ecology and species description, and address the gap in our understanding of this part of marine life. Thus, two flow imaging modalities (in line holographic microscopy and classic optic microscopy), a semi-automated microscopy acquisition procedure, and two three-dimensional (3D) fluorescence microscopy protocols were used. We examined the classic compromises of imaging, including resolution, throughput, and data volume, to evaluate the potential benefits of using such techniques for meiofaunal studies. For ecological surveys, flow imaging could benefit meiobenthos studies, but resolution remains a limiting factor. For taxonomic description, 3D fluorescent imaging added relevant information, considering the time required for preparation and acquisition. The semi-automated motorized microscopy procedure could be used for both purposes according to the versatility of the system.","PeriodicalId":18145,"journal":{"name":"Limnology and Oceanography: Methods","volume":"23 7","pages":"482-499"},"PeriodicalIF":1.9,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144635662","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"地球科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0