Life Science AlliancePub Date : 2024-10-01Print Date: 2024-12-01DOI: 10.26508/lsa.202402802
Ahmet L Tek, Kiyotaka Nagaki, Hümeyra Yıldız Akkamış, Keisuke Tanaka, Hisato Kobayashi
{"title":"Chromosome-specific barcode system with centromeric repeat in cultivated soybean and wild progenitor.","authors":"Ahmet L Tek, Kiyotaka Nagaki, Hümeyra Yıldız Akkamış, Keisuke Tanaka, Hisato Kobayashi","doi":"10.26508/lsa.202402802","DOIUrl":"10.26508/lsa.202402802","url":null,"abstract":"<p><p>Wild soybean <i>Glycine soja</i> is the progenitor of cultivated soybean <i>Glycine max</i> Information on soybean functional centromeres is limited despite extensive genome analysis. These species are an ideal model for studying centromere dynamics for domestication and breeding. We performed a detailed chromatin immunoprecipitation analysis using centromere-specific histone H3 protein to delineate two distinct centromeric DNA sequences with unusual repeating units with monomer sizes of 90-92 bp (CentGm-1) and 413-bp (CentGm-4) shorter and longer than standard nucleosomes. These two unrelated DNA sequences with no sequence similarity are part of functional centromeres in both species. Our results provide a comparison of centromere properties between a cultivated and a wild species under the effect of the same kinetochore protein. Possible sequence homogenization specific to each chromosome could highlight the mechanism for evolutionary conservation of centromeric properties independent of domestication and breeding. Moreover, a unique barcode system to track each chromosome is developed using CentGm-4 units. Our results with a unifying centromere composition model using CentGm-1 and CentGm-4 superfamilies could have far-reaching implications for comparative and evolutionary genome research.</p>","PeriodicalId":18081,"journal":{"name":"Life Science Alliance","volume":"7 12","pages":""},"PeriodicalIF":3.3,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11447526/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142365758","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"TMX5/TXNDC15, a natural trapping mutant of the PDI family is a client of the proteostatic factor ERp44.","authors":"Tatiana Soldà, Carmela Galli, Concetta Guerra, Carolin Hoefner, Maurizio Molinari","doi":"10.26508/lsa.202403047","DOIUrl":"10.26508/lsa.202403047","url":null,"abstract":"<p><p>The ER is the organelle of nucleated cells that produces lipids, sugars, and proteins. More than 20 ER-resident members of the protein disulfide isomerase (PDI) family regulate formation, isomerization, and disassembly of covalent bonds in newly synthesized polypeptides. The PDI family includes few membrane-bound members. Among these, TMX1, TMX2, TMX3, TMX4, and TMX5 belong to the thioredoxin-related transmembrane (TMX) protein family. TMX5 is the least-known member of the family. Here, we establish that TMX5 covalently engages via its active site cysteine residue at position 220 a subset of secretory proteins, mainly single- and multipass Golgi-resident polypeptides. TMX5 also interacts non-covalently, and covalently, via non-catalytic cysteine residues, with the PDI family members PDI, ERp57, and ERp44. The association between TMX5 and ERp44 requires formation of a mixed disulfide between the catalytic cysteine residue 29 of ERp44 and the non-catalytic cysteine residues 114 and/or 124 of TMX5 and controls the ER localization of TMX5 in pre-Golgi compartments. Thus, TMX5 belongs to the family of proteins including Ero1α, Ero1β, Prx4, ERAP1, and SUMF1 that operate in pre-Golgi compartments but lack localization sequences required to position themselves and rely on ERp44 engagement for proper intercompartmental distribution.</p>","PeriodicalId":18081,"journal":{"name":"Life Science Alliance","volume":"7 12","pages":""},"PeriodicalIF":3.3,"publicationDate":"2024-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11443168/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142349453","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Life Science AlliancePub Date : 2024-09-30Print Date: 2024-12-01DOI: 10.26508/lsa.202302083
Joey Llewellyn, Rithvik Baratam, Luka Culig, Isabel Beerman
{"title":"Cellular stress and epigenetic regulation in adult stem cells.","authors":"Joey Llewellyn, Rithvik Baratam, Luka Culig, Isabel Beerman","doi":"10.26508/lsa.202302083","DOIUrl":"10.26508/lsa.202302083","url":null,"abstract":"<p><p>Stem cells are a unique class of cells that possess the ability to differentiate and self-renew, enabling them to repair and replenish tissues. To protect and maintain the potential of stem cells, the cells and the environment surrounding these cells (stem cell niche) are highly responsive and tightly regulated. However, various stresses can affect the stem cells and their niches. These stresses are both systemic and cellular and can arise from intrinsic or extrinsic factors which would have strong implications on overall aging and certain disease states. Therefore, understanding the breadth of drivers, namely epigenetic alterations, involved in cellular stress is important for the development of interventions aimed at maintaining healthy stem cells and tissue homeostasis. In this review, we summarize published findings of epigenetic responses to replicative, oxidative, mechanical, and inflammatory stress on various types of adult stem cells.</p>","PeriodicalId":18081,"journal":{"name":"Life Science Alliance","volume":"7 12","pages":""},"PeriodicalIF":3.3,"publicationDate":"2024-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11443024/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142349450","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Life Science AlliancePub Date : 2024-09-30Print Date: 2024-12-01DOI: 10.26508/lsa.202402650
Nobuhisa Iwata, Satoshi Tsubuki, Misaki Sekiguchi, Kaori Watanabe-Iwata, Yukio Matsuba, Naoko Kamano, Ryo Fujioka, Risa Takamura, Naoto Watamura, Naomasa Kakiya, Naomi Mihira, Takahiro Morito, Keiro Shirotani, David Ma Mann, Andrew C Robinson, Shoko Hashimoto, Hiroki Sasaguri, Takashi Saito, Makoto Higuchi, Takaomi C Saido
{"title":"Metabolic resistance of Aβ3pE-42, a target epitope of the anti-Alzheimer therapeutic antibody, donanemab.","authors":"Nobuhisa Iwata, Satoshi Tsubuki, Misaki Sekiguchi, Kaori Watanabe-Iwata, Yukio Matsuba, Naoko Kamano, Ryo Fujioka, Risa Takamura, Naoto Watamura, Naomasa Kakiya, Naomi Mihira, Takahiro Morito, Keiro Shirotani, David Ma Mann, Andrew C Robinson, Shoko Hashimoto, Hiroki Sasaguri, Takashi Saito, Makoto Higuchi, Takaomi C Saido","doi":"10.26508/lsa.202402650","DOIUrl":"10.26508/lsa.202402650","url":null,"abstract":"<p><p>The amyloid β peptide (Aβ), starting with pyroglutamate (pE) at position 3 and ending at position 42 (Aβ3pE-42), predominantly accumulates in the brains of Alzheimer's disease. Consistently, donanemab, a therapeutic antibody raised against Aβ3pE-42, has been shown to be effective in recent clinical trials. Although the primary Aβ produced physiologically is Aβ1-40/42, an explanation for how and why this physiological Aβ is converted to the pathological form remains elusive. Here, we present experimental evidence that accounts for the aging-associated Aβ3pE-42 deposition: Aβ3pE-42 was metabolically more stable than other Aβx-42 variants; deficiency of neprilysin, the major Aβ-degrading enzyme, induced a relatively selective deposition of Aβ3pE-42 in both APP transgenic and <i>App</i> knock-in mouse brains; Aβ3pE-42 deposition always colocalized with Pittsburgh compound B-positive cored plaques in APP transgenic mouse brains; and under aberrant conditions, such as a significant reduction in neprilysin activity, aminopeptidases, dipeptidyl peptidases, and glutaminyl-peptide cyclotransferase-like were up-regulated in the progression of aging, and a proportion of Aβ1-42 may be processed to Aβ3pE-42. Our findings suggest that anti-Aβ therapies are more effective if given before Aβ3pE-42 deposition.</p>","PeriodicalId":18081,"journal":{"name":"Life Science Alliance","volume":"7 12","pages":""},"PeriodicalIF":3.3,"publicationDate":"2024-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11443169/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142349451","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Life Science AlliancePub Date : 2024-09-20Print Date: 2024-12-01DOI: 10.26508/lsa.202402719
Jore Van Wauwe, Alexia Mahy, Sander Craps, Samaneh Ekhteraei-Tousi, Pieter Vrancaert, Hannelore Kemps, Wouter Dheedene, Rosa Doñate Puertas, Sander Trenson, H Llewelyn Roderick, Manu Beerens, Aernout Luttun
{"title":"PRDM16 determines specification of ventricular cardiomyocytes by suppressing alternative cell fates.","authors":"Jore Van Wauwe, Alexia Mahy, Sander Craps, Samaneh Ekhteraei-Tousi, Pieter Vrancaert, Hannelore Kemps, Wouter Dheedene, Rosa Doñate Puertas, Sander Trenson, H Llewelyn Roderick, Manu Beerens, Aernout Luttun","doi":"10.26508/lsa.202402719","DOIUrl":"10.26508/lsa.202402719","url":null,"abstract":"<p><p>PRDM16 is a transcription factor with histone methyltransferase activity expressed at the earliest stages of cardiac development. Pathogenic mutations in humans lead to cardiomyopathy, conduction abnormalities, and heart failure. PRDM16 is specifically expressed in ventricular but not atrial cardiomyocytes, and its expression declines postnatally. Because in other tissues PRDM16 is best known for its role in binary cell fate decisions, we hypothesized a similar decision-making function in cardiomyocytes. Here, we demonstrated that cardiomyocyte-specific deletion of <i>Prdm16</i> during cardiac development results in contractile dysfunction and abnormal electrophysiology of the postnatal heart, resulting in premature death. By combined RNA+ATAC single-cell sequencing, we found that PRDM16 favors ventricular working cardiomyocyte identity, by opposing the activity of master regulators of ventricular conduction and atrial fate. Myocardial loss of PRDM16 during development resulted in hyperplasia of the (distal) ventricular conduction system. Hence, PRDM16 plays an indispensable role during cardiac development by driving ventricular working cardiomyocyte identity.</p>","PeriodicalId":18081,"journal":{"name":"Life Science Alliance","volume":"7 12","pages":""},"PeriodicalIF":3.3,"publicationDate":"2024-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11415600/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142290414","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Ccrk-Mak/Ick signaling is a ciliary transport regulator essential for retinal photoreceptor survival.","authors":"Taro Chaya, Yamato Maeda, Ryotaro Tsutsumi, Makoto Ando, Yujie Ma, Naoko Kajimura, Teruyuki Tanaka, Takahisa Furukawa","doi":"10.26508/lsa.202402880","DOIUrl":"10.26508/lsa.202402880","url":null,"abstract":"<p><p>Primary cilia are microtubule-based sensory organelles whose dysfunction causes ciliopathies in humans. The formation, function, and maintenance of primary cilia depend crucially on intraflagellar transport (IFT); however, the regulatory mechanisms of IFT at ciliary tips are poorly understood. Here, we identified that the ciliopathy kinase Mak is a ciliary tip-localized IFT regulator that cooperatively acts with the ciliopathy kinase Ick, an IFT regulator. Simultaneous disruption of <i>Mak</i> and <i>Ick</i> resulted in loss of photoreceptor ciliary axonemes and severe retinal degeneration. Gene delivery of <i>Ick</i> and pharmacological inhibition of FGF receptors, Ick negative regulators, ameliorated retinal degeneration in <i>Mak</i> <sup><i>-/-</i></sup> mice. We also identified that Ccrk kinase is an upstream activator of Mak and Ick in retinal photoreceptor cells. Furthermore, the overexpression of Mak, Ick, and Ccrk and pharmacological inhibition of FGF receptors suppressed ciliopathy-related phenotypes caused by cytoplasmic dynein inhibition in cultured cells. Collectively, our results show that the Ccrk-Mak/Ick axis is an IFT regulator essential for retinal photoreceptor maintenance and present activation of Ick as a potential therapeutic approach for retinitis pigmentosa caused by <i>MAK</i> mutations.</p>","PeriodicalId":18081,"journal":{"name":"Life Science Alliance","volume":"7 11","pages":""},"PeriodicalIF":3.3,"publicationDate":"2024-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11412320/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142290412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Shuhei Sakakibara,Yu-Chen Liu,Masakazu Ishikawa,Ryuya Edahiro,Yuya Shirai,Soichiro Haruna,Marwa Ali El Hussien,Zichang Xu,Songling Li,Yuta Yamaguchi,Teruaki Murakami,Takayoshi Morita,Yasuhiro Kato,Haruhiko Hirata,Yoshito Takeda,Fuminori Sugihara,Yoko Naito,Daisuke Motooka,Chao-Yuan Tsai,Chikako Ono,Yoshiharu Matsuura,James B Wing,Hisatake Matsumoto,Hiroshi Ogura,Masato Okada,Atsushi Kumanogoh,Yukinari Okada,Daron M Standley,Hitoshi Kikutani,Daisuke Okuzaki
{"title":"Clonal landscape of autoantibody-secreting plasmablasts in COVID-19 patients.","authors":"Shuhei Sakakibara,Yu-Chen Liu,Masakazu Ishikawa,Ryuya Edahiro,Yuya Shirai,Soichiro Haruna,Marwa Ali El Hussien,Zichang Xu,Songling Li,Yuta Yamaguchi,Teruaki Murakami,Takayoshi Morita,Yasuhiro Kato,Haruhiko Hirata,Yoshito Takeda,Fuminori Sugihara,Yoko Naito,Daisuke Motooka,Chao-Yuan Tsai,Chikako Ono,Yoshiharu Matsuura,James B Wing,Hisatake Matsumoto,Hiroshi Ogura,Masato Okada,Atsushi Kumanogoh,Yukinari Okada,Daron M Standley,Hitoshi Kikutani,Daisuke Okuzaki","doi":"10.26508/lsa.202402774","DOIUrl":"https://doi.org/10.26508/lsa.202402774","url":null,"abstract":"Whereas severe COVID-19 is often associated with elevated autoantibody titers, the underlying mechanism behind their generation has remained unclear. Here we report clonal composition and diversity of autoantibodies in humoral response to SARS-CoV-2. Immunoglobulin repertoire analysis and characterization of plasmablast-derived monoclonal antibodies uncovered clonal expansion of plasmablasts producing cardiolipin (CL)-reactive autoantibodies. Half of the expanded CL-reactive clones exhibited strong binding to SARS-CoV-2 antigens. One such clone, CoV1804, was reactive to both CL and viral nucleocapsid (N), and further showed anti-nucleolar activity in human cells. Notably, antibodies sharing genetic features with CoV1804 were identified in COVID-19 patient-derived immunoglobulins, thereby constituting a novel public antibody. These public autoantibodies had numerous mutations that unambiguously enhanced anti-N reactivity, when causing fluctuations in anti-CL reactivity along with the acquisition of additional self-reactivities, such as anti-nucleolar activity, in the progeny. Thus, potentially CL-reactive precursors may have developed multiple self-reactivities through clonal selection, expansion, and somatic hypermutation driven by viral antigens. Our results revealed the nature of autoantibody production during COVID-19 and provided novel insights into the origin of virus-induced autoantibodies.","PeriodicalId":18081,"journal":{"name":"Life Science Alliance","volume":"50 1","pages":""},"PeriodicalIF":4.4,"publicationDate":"2024-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142266498","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Christelle Guillermier,Naveen Vg Kumar,Ronan C Bracken,Diana Alvarez,John O'Keefe,Aditi Gurkar,Jonathan D Brown,Matthew L Steinhauser
{"title":"Nanoscale imaging of DNA-RNA identifies transcriptional plasticity at heterochromatin.","authors":"Christelle Guillermier,Naveen Vg Kumar,Ronan C Bracken,Diana Alvarez,John O'Keefe,Aditi Gurkar,Jonathan D Brown,Matthew L Steinhauser","doi":"10.26508/lsa.202402849","DOIUrl":"https://doi.org/10.26508/lsa.202402849","url":null,"abstract":"The three-dimensional structure of DNA is a biophysical determinant of transcription. The density of chromatin condensation is one determinant of transcriptional output. Chromatin condensation is generally viewed as enforcing transcriptional suppression, and therefore, transcriptional output should be inversely proportional to DNA compaction. We coupled stable isotope tracers with multi-isotope imaging mass spectrometry to quantify and image nanovolumetric relationships between DNA density and newly made RNA within individual nuclei. Proliferative cell lines and cycling cells in the murine small intestine unexpectedly demonstrated no consistent relationship between DNA density and newly made RNA, even though localized examples of this phenomenon were detected at nuclear-cytoplasmic transitions. In contrast, non-dividing hepatocytes demonstrated global reduction in newly made RNA and an inverse relationship between DNA density and transcription, driven by DNA condensates at the nuclear periphery devoid of newly made RNA. Collectively, these data support an evolving model of transcriptional plasticity that extends at least to a subset of chromatin at the extreme of condensation as expected of heterochromatin.","PeriodicalId":18081,"journal":{"name":"Life Science Alliance","volume":"26 1","pages":""},"PeriodicalIF":4.4,"publicationDate":"2024-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142266499","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Sun H Park,Shunsuke Tsuzuki,Kelly F Contino,Jenna Ollodart,Matthew R Eber,Yang Yu,Laiton R Steele,Hiroyuki Inaba,Yuko Kamata,Takahiro Kimura,Ilsa Coleman,Peter S Nelson,Enriqueta Muñoz-Islas,Juan Miguel Jiménez-Andrade,Thomas J Martin,Kimberly D Mackenzie,Jennifer R Stratton,Fang-Chi Hsu,Christopher M Peters,Yusuke Shiozawa
{"title":"Crosstalk between bone metastatic cancer cells and sensory nerves in bone metastatic progression.","authors":"Sun H Park,Shunsuke Tsuzuki,Kelly F Contino,Jenna Ollodart,Matthew R Eber,Yang Yu,Laiton R Steele,Hiroyuki Inaba,Yuko Kamata,Takahiro Kimura,Ilsa Coleman,Peter S Nelson,Enriqueta Muñoz-Islas,Juan Miguel Jiménez-Andrade,Thomas J Martin,Kimberly D Mackenzie,Jennifer R Stratton,Fang-Chi Hsu,Christopher M Peters,Yusuke Shiozawa","doi":"10.26508/lsa.202302041","DOIUrl":"https://doi.org/10.26508/lsa.202302041","url":null,"abstract":"Although the role of peripheral nerves in cancer progression has been appreciated, little is known regarding cancer/sensory nerve crosstalk and its contribution to bone metastasis and associated pain. In this study, we revealed that the cancer/sensory nerve crosstalk plays a crucial role in bone metastatic progression. We found that (i) periosteal sensory nerves expressing calcitonin gene-related peptide (CGRP) are enriched in mice with bone metastasis; (ii) cancer patients with bone metastasis have elevated CGRP serum levels; (iii) bone metastatic patient tumor samples express elevated calcitonin receptor-like receptor (CRLR, a CGRP receptor component); (iv) higher CRLR levels in cancer patients are negatively correlated with recurrence-free survival; (v) CGRP induces cancer cell proliferation through the CRLR/p38/HSP27 pathway; and (vi) blocking sensory neuron-derived CGRP reduces cancer cell proliferation in vitro and bone metastatic progression in vivo. This suggests that CGRP-expressing sensory nerves are involved in bone metastatic progression and that the CGRP/CRLR axis may serve as a potential therapeutic target for bone metastasis.","PeriodicalId":18081,"journal":{"name":"Life Science Alliance","volume":"197 1","pages":""},"PeriodicalIF":4.4,"publicationDate":"2024-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142266500","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Miriam Valera-Alberni,Pallas Yao,Silvia Romero-Sanz,Anne Lanjuin,William B Mair
{"title":"Novel imaging tools to study mitochondrial morphology in Caenorhabditis elegans.","authors":"Miriam Valera-Alberni,Pallas Yao,Silvia Romero-Sanz,Anne Lanjuin,William B Mair","doi":"10.26508/lsa.202402918","DOIUrl":"https://doi.org/10.26508/lsa.202402918","url":null,"abstract":"Mitochondria exhibit a close interplay between their structure and function. Understanding this intricate relationship requires advanced imaging techniques that can capture the dynamic nature of mitochondria and their impact on cellular processes. However, much of the work on mitochondrial dynamics has been performed in single celled organisms or in vitro cell culture. Here, we introduce novel genetic tools for live imaging of mitochondrial morphology in the nematode Caenorhabditis elegans, addressing a pressing need for advanced techniques in studying organelle dynamics within live intact multicellular organisms. Through a comprehensive analysis, we directly compare our tools with existing methods, demonstrating their advantages for visualizing mitochondrial morphology and contrasting their impact on organismal physiology. We reveal limitations of conventional techniques, whereas showcasing the utility and versatility of our approaches, including endogenous CRISPR tags and ectopic labeling. By providing a guide for selecting the most suitable tools based on experimental goals, our work advances mitochondrial research in C. elegans and enhances the strategic integration of diverse imaging modalities for a holistic understanding of organelle dynamics in living organisms.","PeriodicalId":18081,"journal":{"name":"Life Science Alliance","volume":"42 1","pages":""},"PeriodicalIF":4.4,"publicationDate":"2024-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142222655","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}