Immunology & Cell Biology最新文献_第3页

Unraveling the origins of pathogenic CXCL13+ helper T cells in systemic lupus erythematosus 揭示系统性红斑狼疮中致病性 CXCL13+ 辅助 T 细胞的起源。

IF 3.2 4区医学

Immunology & Cell Biology Pub Date : 2024-09-01 DOI: 10.1111/imcb.12818

Sam Nettelfield, Zhian Chen

引用次数: 0

Improving undergraduate education in immunology through assessment of interdisciplinary scientific knowledge. 通过评估跨学科科学知识改进免疫学本科教育。

IF 3.2 4区医学

Immunology & Cell Biology Pub Date : 2024-08-20 DOI: 10.1111/imcb.12815

Joshua J Baty, Isha Patel, Jared P Taylor, Connor Graben, Jill Deaver, Louis B Justement, Heather A Bruns

{"title":"Improving undergraduate education in immunology through assessment of interdisciplinary scientific knowledge.","authors":"Joshua J Baty, Isha Patel, Jared P Taylor, Connor Graben, Jill Deaver, Louis B Justement, Heather A Bruns","doi":"10.1111/imcb.12815","DOIUrl":"https://doi.org/10.1111/imcb.12815","url":null,"abstract":"The interdisciplinary nature of immunology can make studying not only engaging but also challenging, as understanding immunologic processes and immune system components requires foundational knowledge from several science disciplines. The University of Alabama at Birmingham has a unique, 4-year, Undergraduate Immunology Program (UIP) that provides a comprehensive curriculum in immunology that includes five core courses starting in the second year, at which point, students are in the process of completing basic science sequences. For this study, students in courses across the UIP curriculum were asked to identify basic science topics that relate to four immunology concepts. In addition, students were surveyed on their confidence in understanding each of the basic science topics and were asked to identify the course in which they felt that they had fully learned the topic. Data from this study did not demonstrate a change in students' interdisciplinary science competency from the second to fourth year. Importantly, students reported that they fully understood 11 out of 12 basic science concepts in courses offered in their first and second years, with confidence in basic science topics significantly improving from the second to third year. The lack of demonstrated improvement in interdisciplinary understanding across the curriculum may be attributed to the fact that students are able to integrate basic science topics with foundational immunologic concepts as early as their second year. Importantly, these findings suggest that the integration or review of basic science topics in an immunology course may improve students' comprehension of foundational immunology concepts and interdisciplinary science competency.","PeriodicalId":179,"journal":{"name":"Immunology & Cell Biology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142007951","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Myddosomes in Toll-like receptor signaling—one to bind and rule them all Toll样受体信号中的多糖体--结合并统治一切。

IF 3.2 4区医学

Immunology & Cell Biology Pub Date : 2024-08-19 DOI: 10.1111/imcb.12816

Carmen D Mathmann, Thomas E Schultz, Leslie C Domínguez Cadena, Antje Blumenthal

{"title":"Myddosomes in Toll-like receptor signaling—one to bind and rule them all","authors":"Carmen D Mathmann, Thomas E Schultz, Leslie C Domínguez Cadena, Antje Blumenthal","doi":"10.1111/imcb.12816","DOIUrl":"10.1111/imcb.12816","url":null,"abstract":"Toll-like receptors (TLRs) are innate immune sensors for the presence of pathogens and endogenous danger signals. TLR activation results in conserved intracellular signaling events that orchestrate inflammation and antimicrobial defense. While the identity and interplay of key TLR signaling components are well established, how these largely cytosolic proteins are physically connected is not well understood. For the activation of conserved intracellular signaling events, most TLRs engage the adapter MyD88 (myeloid differentiation primary response 88), which assembles into higher-order protein complexes, myddosomes. In their recent publication, Fisch et al. present evidence that oligomeric myddosomes detach from initiating TLRs and evolve into larger scaffolds that dynamically assemble not only proximal but also distal cytosolic elements required to execute the entire cascade of the TLR–MyD88 signaling pathway. Coinciding with decline in TLR signaling over time, myddosomes progressively recruit autophagy machinery that mediates myddosome clearance. These findings expand the current understanding of TLR signaling by positioning myddosomes as the central structural element that physically assembles the key executors and regulators of TLR–MyD88-dependent intracellular signaling cascades.","PeriodicalId":179,"journal":{"name":"Immunology & Cell Biology","volume":"102 9","pages":"752-756"},"PeriodicalIF":3.2,"publicationDate":"2024-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/imcb.12816","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141999068","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Immunology in Practice: a modular framework to support Master of Science students conference attendance and engagement. 免疫学实践：支持理科硕士生参加和参与会议的模块框架。

IF 3.2 4区医学

Immunology & Cell Biology Pub Date : 2024-08-14 DOI: 10.1111/imcb.12814

Malgorzata Trela, Sophie Rutschmann

{"title":"Immunology in Practice: a modular framework to support Master of Science students conference attendance and engagement.","authors":"Malgorzata Trela, Sophie Rutschmann","doi":"10.1111/imcb.12814","DOIUrl":"https://doi.org/10.1111/imcb.12814","url":null,"abstract":"Sharing a passion for the advancement of the discipline, the scientific community provides an authentic environment for new members to acquire the knowledge and develop the professional identity needed for their future careers. Supporting opportunities for higher education students to participate in this community can complement their classroom-based education and be extremely beneficial to their learning. Situated in the authentic environment of the scientific community, conferences are organized events where professionals meet to advance their discipline, and which have been shown to provide unique learning opportunities for university students. Here we present a modular framework created to support Imperial College London's Master of Science in Immunology students' attendance at the British Society for Immunology Annual Congress. The module's evaluation indicates an overall students' satisfaction with the content, organization, teaching, assessment, feedback and community aspects of the framework and draws attention to areas of potential improvements. Furthermore, the data emphasize the importance of preconference preparation, of academic mentoring and discusses the role of peer support. Finally, the data highlight the benefits for students of discovering the true breadth and depth of their discipline, of interacting with members of the community and how these contribute to the development of their professional identity.","PeriodicalId":179,"journal":{"name":"Immunology & Cell Biology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141981340","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A combined interactive online simulation and face-to-face laboratory enable undergraduate student proficiency in hemocytometer use, cell density and viability calculations. 互动式在线模拟和面对面实验室相结合，使本科生能够熟练掌握血细胞计数器的使用、细胞密度和存活率的计算。

IF 3.2 4区医学

Immunology & Cell Biology Pub Date : 2024-07-30 DOI: 10.1111/imcb.12813

Maurizio Costabile, Sheree Bailey, Gareth Denyer

{"title":"A combined interactive online simulation and face-to-face laboratory enable undergraduate student proficiency in hemocytometer use, cell density and viability calculations.","authors":"Maurizio Costabile, Sheree Bailey, Gareth Denyer","doi":"10.1111/imcb.12813","DOIUrl":"https://doi.org/10.1111/imcb.12813","url":null,"abstract":"A hemocytometer is a key piece of laboratory equipment typically used in diagnostic and immunology research laboratories to enumerate white blood cells. The accurate quantification of cell density is essential to ensure accurate numbers of cells are added to assays to generate valid data. Hence, learning to correctly use a hemocytometer is a critical skill for all undergraduate immunology students. However, this skill can be challenging to learn because of students' unfamiliarity with correct cell identification, differentiating viable versus dead cells and mathematical proficiency in calculating cell density and viability. To address these issues, we developed an interactive computer simulation that replicated all aspects of a Neubauer-style hemocytometer. This simulation was used to teach second-year undergraduate immunology students before a face-to-face (F-2-F) laboratory exercise where these skills were applied. Using a mixed methods approach, student performance and feedback were collected on broad aspects of the intervention and its benefits to the F-2-F setting. The approach was found to be extremely successful with all measures indicating a significant impact of the virtual hemocytometer on student learning, understanding and confidence. We suggest that integrating an online simulation to teach students the fundamentals of hemocytometer use and calculations is a valuable educational aid for learning this important skill.","PeriodicalId":179,"journal":{"name":"Immunology & Cell Biology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141791389","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Spatial resolution of the head and neck cancer tumor microenvironment to identify tumor and stromal features associated with therapy response 头颈癌肿瘤微环境的空间分辨率，以确定与治疗反应相关的肿瘤和基质特征。

IF 3.2 4区医学

Immunology & Cell Biology Pub Date : 2024-07-24 DOI: 10.1111/imcb.12811

Naomi Berrell, James Monkman, Meg Donovan, Tony Blick, Ken O'Byrne, Rahul Ladwa, Chin Wee Tan, Arutha Kulasinghe

{"title":"Spatial resolution of the head and neck cancer tumor microenvironment to identify tumor and stromal features associated with therapy response","authors":"Naomi Berrell, James Monkman, Meg Donovan, Tony Blick, Ken O'Byrne, Rahul Ladwa, Chin Wee Tan, Arutha Kulasinghe","doi":"10.1111/imcb.12811","DOIUrl":"10.1111/imcb.12811","url":null,"abstract":"Head and neck cancer (HNC) is the seventh most common cancer globally, resulting in 440 000 deaths per year. While there have been advancements in chemoradiotherapy and surgery, relapse occurs in more than half of HNCs, and these patients have a median survival of 10 months and a 2-year survival of < 20%. Only a subset of patients displays durable benefits from immunotherapies in metastatic and recurrent HNC, making it critical to understand the tumor microenvironment (TME) underpinning therapy responses in HNC. To recognize biological differences within the TME that may be predictive of immunotherapy response, we applied cutting-edge geospatial whole-transcriptome profiling (NanoString GeoMx Digital Spatial Profiler) and spatial proteomics profiling (Akoya PhenoCycler-Fusion) on a tumor microarray consisting of 25 cores from 12 patients that included 4 immunotherapy-unresponsive (8 cores) and 2 immunotherapy-responsive patients (5 cores), as well as 6 immunotherapy naïve patients (12 cores). Through high-plex, regional-based transcriptomic mapping of the tumor and TME, pathways involved with the complement system and hypoxia were identified to be differentially expressed in patients who went on to experience a poor immunotherapy response. Single-cell, targeted proteomic analysis found that immune cell infiltration of the cancer cell mass and interactions of CD8 T cells with tumor and other immune cells were associated with positive immunotherapy response. The relative abundance of specific tumor phenotypes and their interactions with various immune cells was identified to be different between response groups. This study demonstrates how spatial transcriptomics and proteomics can resolve novel alterations in the TME of HNC that may contribute to therapy sensitivity and resistance.","PeriodicalId":179,"journal":{"name":"Immunology & Cell Biology","volume":"102 9","pages":"830-846"},"PeriodicalIF":3.2,"publicationDate":"2024-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/imcb.12811","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141756012","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The up-regulation of PD-L1 during boningmycin-induced senescence in human cancer cells depends on the activation of the JAK/STAT signaling pathway mediated by SASP 在骨化霉素诱导的人类癌细胞衰老过程中，PD-L1的上调取决于SASP介导的JAK/STAT信号通路的激活。

IF 3.2 4区医学

Immunology & Cell Biology Pub Date : 2024-07-23 DOI: 10.1111/imcb.12812

Yang Chen, Jiajia Shen, Xiaoli Zhao, Qiyang He, Juan Zhang

{"title":"The up-regulation of PD-L1 during boningmycin-induced senescence in human cancer cells depends on the activation of the JAK/STAT signaling pathway mediated by SASP","authors":"Yang Chen, Jiajia Shen, Xiaoli Zhao, Qiyang He, Juan Zhang","doi":"10.1111/imcb.12812","DOIUrl":"10.1111/imcb.12812","url":null,"abstract":"Therapy-induced senescence can regulate both the innate and adaptive immune systems, thereby affecting therapeutic efficacy. Bleomycin is a major component of combined chemotherapy regimens, utilized for the treatment of multiple tumors, whereas pulmonary toxicity severely restricts its clinical benefits. As a member of the bleomycin family, boningmycin (BON) exhibits potent anticancer activity with minimal pulmonary toxicity, making it a potential alternative to bleomycin. Low concentrations of BON can induce senescence, but the impact of BON-induced senescence on anticancer immunity remains unclear. This study investigates the effects of BON-induced senescence on PD-L1 expression and the underlying mechanisms in human cancer cells. Firstly, the elevation of PD-L1 protein during BON-induced senescence was confirmed by a senescence β-galactosidase staining assay, detection of the senescence-associated secretory phenotype (SASP), western blot and flow cytometry in human lung cancer NCI-H460 cells and breast cancer MDA-MB-231 cells. Subsequently, it was shown that the increase in PD-L1 protein is mediated by SASP, as evidenced by the use of conditional media, knockdown of cyclic GMP-AMP synthase and inhibition of stimulator of interferon genes. Ultimately, it was demonstrated that SASP-mediated PD-L1 up-regulation is dependent on the activation of the JAK/STAT pathway through the use of specific inhibitors and siRNAs. These findings clarify the impact of BON-induced senescence on PD-L1 expression and may contribute to the optimization of the therapeutic efficacy of bleomycin-related compounds and the clinical transformation of BON.","PeriodicalId":179,"journal":{"name":"Immunology & Cell Biology","volume":"102 9","pages":"847-859"},"PeriodicalIF":3.2,"publicationDate":"2024-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141750658","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Adipokine receptor expression in mast cells is altered by specific ligands and proinflammatory cytokines 特定配体和促炎细胞因子会改变肥大细胞中脂肪因子受体的表达。

IF 3.2 4区医学

Immunology & Cell Biology Pub Date : 2024-07-16 DOI: 10.1111/imcb.12809

Paulina Żelechowska, Magdalena Wiktorska, Elżbieta Kozłowska, Justyna Agier

{"title":"Adipokine receptor expression in mast cells is altered by specific ligands and proinflammatory cytokines","authors":"Paulina Żelechowska, Magdalena Wiktorska, Elżbieta Kozłowska, Justyna Agier","doi":"10.1111/imcb.12809","DOIUrl":"10.1111/imcb.12809","url":null,"abstract":"Adipokines play essential roles in regulating a range of biological processes, but growing evidence indicates that they are also fundamental in immunological mechanisms and, primarily, inflammatory responses. Adipokines mediate their actions through specific receptors. However, although adipokine receptors are widely distributed in many cell and tissue types, limited data are available on their expression in mast cells (MCs) and, consequently, adipokine's significance in the modulation of MC activity within the tissues. In this study, we demonstrate that rat peritoneal MCs constitutively express the leptin receptor (i.e. LEPR), adiponectin receptors (i.e. ADIPOR1 and ADIPOR2) and the chemerin receptor (i.e. CMKLR1). We also found that LEPR, ADIPOR1, ADIPOR2 and CMKLR1 expression in MCs changes in response to stimulation by their specific ligands and some cytokines with potent proinflammatory properties. Furthermore, the involvement of intracellular signaling molecules in leptin-, adiponectin- and chemerin-induced MC response was analyzed. Overall, our findings suggest that adipokines leptin, adiponectin and chemerin can significantly affect the activity of MCs in various processes, especially during inflammation. These observations may contribute significantly to understanding the relationship between adipokines, immune mechanisms and diseases or conditions with an inflammatory component.","PeriodicalId":179,"journal":{"name":"Immunology & Cell Biology","volume":"102 9","pages":"817-829"},"PeriodicalIF":3.2,"publicationDate":"2024-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141625494","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Computational estimation of clonal diversity in autoimmunity 自身免疫中克隆多样性的计算估算。

IF 3.2 4区医学

Immunology & Cell Biology Pub Date : 2024-07-15 DOI: 10.1111/imcb.12801

Zewen Kelvin Tuong, Rohan van der Merwe, Pablo F Canete, Jonathan A Roco

引用次数: 0

Type I interferon induced during chronic viral infection favors B-cell development in the thymus 慢性病毒感染期间诱导的 I 型干扰素有利于胸腺中 B 细胞的发育。

IF 3.2 4区医学

Immunology & Cell Biology Pub Date : 2024-07-15 DOI: 10.1111/imcb.12808

Stefanie F Valbon, Marie-Eve Lebel, H Alex Feldman, Stephanie A Condotta, Mengqi Dong, Daniela Giordano, Stephen N Waggoner, Heather J Melichar, Martin J Richer

{"title":"Type I interferon induced during chronic viral infection favors B-cell development in the thymus","authors":"Stefanie F Valbon, Marie-Eve Lebel, H Alex Feldman, Stephanie A Condotta, Mengqi Dong, Daniela Giordano, Stephen N Waggoner, Heather J Melichar, Martin J Richer","doi":"10.1111/imcb.12808","DOIUrl":"10.1111/imcb.12808","url":null,"abstract":"Chronic viral infections cause thymic involution yet the potential for broader, longer-term impact on thymic composition remains unexplored. Here we show that chronic, but not acute, lymphocytic choriomeningitis virus infection promotes a unique population of immature B cells in the thymus. We show that chronic viral infection promotes signals within the thymus, including the expression of B-cell activating factor (BAFF), that favor the maturation of this population as these cells acquire expression of CD19 and immunoglobulin M. Mechanistically, type I interferon (IFN-I), predominantly IFNβ, signals to thymic hematopoietic cells, strongly delaying T-cell development at the earliest precursor stage. Furthermore, IFN-I signaling to the nonhematopoietic compartment provides a second signal essential to favor B-cell differentiation and maturation within the thymus. Importantly, chronic infection yields changes in the B-cell population for at least 50 days following infection, long after thymic atrophy has subsided. Thus, the inflammatory milieu induced by chronic viral infection has a profound, and long-lasting, effect on thymic composition leading to the generation of a novel population of thymic B cells.","PeriodicalId":179,"journal":{"name":"Immunology & Cell Biology","volume":"102 9","pages":"801-816"},"PeriodicalIF":3.2,"publicationDate":"2024-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11444890/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141618791","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0