Laboratory Animal Research最新文献

{"title":"Comparison of immunophenotypes between Rag2 knockout mice derived from two different sources.","authors":"Yu Jeong Roh,&nbsp;Jeong Eun Gong,&nbsp;Ji Eun Kim,&nbsp;You Jeong Jin,&nbsp;Hee Jin Song,&nbsp;Ayun Seol,&nbsp;Jumin Park,&nbsp;Yong Lim,&nbsp;Dae Youn Hwang","doi":"10.1186/s42826-023-00153-8","DOIUrl":"https://doi.org/10.1186/s42826-023-00153-8","url":null,"abstract":"<p><strong>Background: </strong>Recombination activating gene2 (Rag2) knockout (KO) mice are used widely in various research fields, including vaccine development, transplantation studies, and hematopoiesis research, but few studies have compared their phenotypes. This study examined whether there were differences in the immunophenotypes between Rag2 KO mice derived from different sources. In particular, the changes in the organ weight, histological structure, and subpopulation of T and B cells were compared in the spleen and thymus of C57BL/6-Rag2^em1hwl/Korl (Rag2/Korl KO) and B6.Cg-Rag2^tm1.1Cgn/J (Rag2/J KO) mice.</p><p><strong>Results: </strong>The weight of the spleen and thymus similarly decreased in the Rag2/Korl and Rag2/J KO mice compared to their wild type (WT) mice, even though the other organs were kept at the same weight. A slight difference between the Rag2/Korl and Rag2/J KO group were detected in the number of white blood cells (WBC), lymphocytes (LYM), red cell distribution width (RDW), and platelets (PLT). In addition, the white pulp of the spleen and the cortex region of the thymus decreased in both Rag2 KO mice compared to WT mice. On the other hand, significant differences in the number of CD8⁺ T and B cell subpopulations between WT and Rag2 KO mice were observed between Rag2/Korl and Rag2/J KO group, while the CD4⁺ T subpopulation was maintained similarly in both groups.</p><p><strong>Conclusions: </strong>These results suggest that Rag2/Korl and Rag2/J KO mice exhibit similar immunophenotypes in the spleen and thymus except for the differences in the number of CD8⁺ T and B cell subpopulations.</p>","PeriodicalId":17993,"journal":{"name":"Laboratory Animal Research","volume":"39 1","pages":"2"},"PeriodicalIF":2.9,"publicationDate":"2023-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9832259/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10520537","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Improvement of the intestinal epithelial barrier during laxative effects of phlorotannin in loperamide-induced constipation of SD rats.","authors":"Ji Eun Kim,&nbsp;Hee Jin Song,&nbsp;Yun Ju Choi,&nbsp;You Jeong Jin,&nbsp;Yu Jeong Roh,&nbsp;Ayun Seol,&nbsp;So Hae Park,&nbsp;Ju Min Park,&nbsp;Hyun Gu Kang,&nbsp;Dae Youn Hwang","doi":"10.1186/s42826-022-00152-1","DOIUrl":"https://doi.org/10.1186/s42826-022-00152-1","url":null,"abstract":"<p><strong>Background: </strong>Disruptions of the intestinal epithelial barrier (IEB) are frequently observed in various digestive diseases, including irritable bowel syndrome (IBS) and inflammatory bowel disease (IBD). This study assessed the improvement in the IEB during the laxative activity of phlorotannin (Pt) harvested from Ecklonia cava in constipation by examining the changes in the expression of the regulatory proteins for the tight junction (TJ) and adherens junction (AJ), and inflammatory cytokines in Sprague Dawley (SD) rats with loperamide (Lm)-induced constipation after a Pt treatment.</p><p><strong>Results: </strong>The Pt treatment induced laxative activity, including the improvement of feces-related parameters, gastrointestinal transit rate, and histological structure of the mid colon in Lm-treated SD rats. In addition, significant recovery effects were detected in the histology of IEB, including the mucus layer, epithelial cells, and lamina propria in the mid colon of Lm + Pt treated SD rats. The expression levels of E-cadherin and p120-catenin for AJ and the ZO-1, occludin, and Claudin-1 genes for TJ in epithelial cells were improved remarkably after the Pt treatment, but the rate of increase was different. Furthermore, the Pt treatment increased the expression level of several inflammatory cytokines, such as TNF-α, IL-6, IL-1β, IL-13, and IL-4 in Lm + Pt treated SD rats.</p><p><strong>Conclusions: </strong>These results provide the first evidence that the laxative activity of Pt in SD rats with Lm-induced constipation phenotypes involve improvements in the IEB.</p>","PeriodicalId":17993,"journal":{"name":"Laboratory Animal Research","volume":"39 1","pages":"1"},"PeriodicalIF":2.9,"publicationDate":"2023-01-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9808941/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10545559","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chlorogenic acid modulates the ubiquitin-proteasome system in stroke animal model.","authors":"Murad-Ali Shah,&nbsp;Ju-Bin Kang,&nbsp;Phil-Ok Koh","doi":"10.1186/s42826-022-00151-2","DOIUrl":"https://doi.org/10.1186/s42826-022-00151-2","url":null,"abstract":"<p><strong>Background: </strong>Chlorogenic acid, a phenolic compound, has potent antioxidant and neuroprotective properties. The ubiquitin-proteasome system is an important regulators of neurodevelopment and modulators of neuronal function. This system is associated with neurodevelopment and neurotransmission through degradation and removal of damaged proteins. Activation of the ubiquitin-proteasome system is a critical factor in preventing cell death. We have previously reported a decrease in the activity of the ubiquitin-proteasome system during cerebral ischemia. This study investigated whether chlorogenic acid regulates the ubiquitin-proteasome system in an animal stroke model. In adult rats, middle cerebral artery occlusion (MCAO) surgery was performed to induce focal cerebral ischemia. Chlorogenic acid (30 mg/kg) or normal saline was injected into the abdominal cavity 2 h after MCAO surgery, and cerebral cortex tissues were collected 24 h after MCAO damage.</p><p><strong>Results: </strong>Chlorogenic acid attenuated neurobehavioral disorders and histopathological changes caused by MCAO damage. We identified the decreases in ubiquitin C-terminal hydrolase L1, ubiquitin thioesterase OTUB1, proteasome subunit α type 1, proteasome subunit α type 3, and proteasome subunit β type 4 expression using a proteomics approach in MCAO animals. The decrease in these proteins was alleviated by chlorogenic acid. In addition, the results of reverse transcription-polymerase chain reaction confirmed these changes. The identified proteins were markedly reduced in MCAO damage, while chlorogenic acid prevented these reductions induced by MCAO. The decrease of ubiquitin-proteasome system proteins in ischemic damage was associated with neuronal apoptosis.</p><p><strong>Conclusions: </strong>Our results showed that chlorogenic acid regulates ubiquitin-proteasome system proteins and protects cortical neurons from neuronal damage. These results provide evidence that chlorogenic acid has neuroprotective effects and maintains the ubiquitin-proteasome system in ischemic brain injury.</p>","PeriodicalId":17993,"journal":{"name":"Laboratory Animal Research","volume":"38 1","pages":"41"},"PeriodicalIF":2.9,"publicationDate":"2022-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9768937/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10419563","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anesthesia and analgesia for common research models of adult mice.","authors":"Siavash Ahmadi-Noorbakhsh, Mohammad Farajli Abbasi, Maedeh Ghasemi, Gholamreza Bayat, Nahid Davoodian, Ehsan Sharif-Paghaleh, Seyedeh Mahsa Poormoosavi, Melika Rafizadeh, Maryam Maleki, Hesamaddin Shirzad-Aski, Hossein Kargar Jahromi, Masoomeh Dadkhah, Bahman Khalvati, Tahereh Safari, Mohammad Amin Behmanesh, Seyed Esmaeil Khoshnam, Gholamreza Houshmand, Sayyed Alireza Talaei","doi":"10.1186/s42826-022-00150-3","DOIUrl":"10.1186/s42826-022-00150-3","url":null,"abstract":"<p><p>Anesthesia and analgesia are major components of many interventional studies on laboratory animals. However, various studies have shown improper reporting or use of anesthetics/analgesics in research proposals and published articles. In many cases, it seems \"anesthesia\" and \"analgesia\" are used interchangeably, while they are referring to two different concepts. Not only this is an unethical practice, but also it may be one of the reasons for the proven suboptimal quality of many animal researches. This is a widespread problem among investigations on various species of animals. However, it could be imagined that it may be more prevalent for the most common species of laboratory animals, such as the laboratory mice. In this review, proper anesthetic/analgesic methods for routine procedures on laboratory mice are discussed. We considered the available literature and critically reviewed their anesthetic/analgesic methods. Detailed dosing and pharmacological information for the relevant drugs are provided and some of the drugs' side effects are discussed. This paper provides the necessary data for an informed choice of anesthetic/analgesic methods in some routine procedures on laboratory mice.</p>","PeriodicalId":17993,"journal":{"name":"Laboratory Animal Research","volume":"38 1","pages":"40"},"PeriodicalIF":2.7,"publicationDate":"2022-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9746144/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10361177","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Resistance exercise training-induced skeletal muscle strength provides protective effects on high-fat-diet-induced metabolic stress in mice.","authors":"Hye Jin Kim,&nbsp;Youn Ju Kim,&nbsp;Il Yong Kim,&nbsp;Je Kyung Seong","doi":"10.1186/s42826-022-00145-0","DOIUrl":"https://doi.org/10.1186/s42826-022-00145-0","url":null,"abstract":"<p><strong>Background: </strong>Resistance exercise training is known to improve metabolic disorders, such as obesity and type2 diabetes. In this study, we investigated whether the beneficial effects of resistance exercise training persisted even after the discontinuation of training with high-fat diet (HFD)-induced metabolic stress. We further evaluated whether the improvement in skeletal muscle strength and endurance by training were correlated with improved metabolism. Eight-week-old male C57BL/6N mice were divided into groups that remained sedentary or had access to daily resistance exercise via ladder climbing for 8 weeks. Trained and untrained mice were fed an HFD for 1 week after the exercise training intervention (n = 5-8 per group).</p><p><strong>Results: </strong>Resistance exercise-trained mice had a lean phenotype and counteracted diet-induced obesity and glucose tolerance, even after exercise cessation. Grip strength was significantly inversely correlated with the body weight, fat mass, and glucose tolerance. However, hanging time was significantly inversely correlated with body weight only.</p><p><strong>Conclusions: </strong>These results have strong implications for the preventive effect of resistance exercise-induced metabolic improvement by enhancing skeletal muscle strength rather than endurance.</p>","PeriodicalId":17993,"journal":{"name":"Laboratory Animal Research","volume":"38 1","pages":"36"},"PeriodicalIF":2.9,"publicationDate":"2022-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9716768/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10327760","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Time progression and regional expression of brain oxidative stress induced by obstructive jaundice in rats.","authors":"Konstantinos Lilimpakis,&nbsp;Aidona Tsepelaki,&nbsp;Electra Kalaitzopoulou,&nbsp;Dimitrios Zisimopoulos,&nbsp;Polyxeni Papadea,&nbsp;Marianna Skipitari,&nbsp;Athina Varemmenou,&nbsp;Apostolos Aggelis,&nbsp;Constantine Vagianos,&nbsp;Constantine Constantoyannis,&nbsp;Christos D Georgiou","doi":"10.1186/s42826-022-00146-z","DOIUrl":"https://doi.org/10.1186/s42826-022-00146-z","url":null,"abstract":"<p><strong>Background: </strong>Obstructive jaundice induces oxidative changes in the brain parenchyma and plays significant role in clinical manifestations of hepatic encephalopathy. We aim to study the progression of the brain oxidative status over time and the differences of its pattern over the hemispheres, the brainstem and the cerebellum. We use an experimental model in rats and measuring the oxidative stress (OS) specific biomarkers protein malondialdehyde (PrMDA) and protein carbonyls (PrC = O).</p><p><strong>Results: </strong>Hyperbilirubinemia has been confirmed in all study groups as the result of common bile duct obstruction. We confirmed increase in both PrMDA and PrC = O biomarkers levels with different type of changes over time. We also confirmed that the oxidative process develops differently in each of the brain areas in study.</p><p><strong>Conclusions: </strong>The present study confirms the progressive increase in OS in all brain areas studied using markers indicative of cumulative protein modification.</p>","PeriodicalId":17993,"journal":{"name":"Laboratory Animal Research","volume":"38 1","pages":"35"},"PeriodicalIF":2.9,"publicationDate":"2022-11-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9701014/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10327944","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The current status and history of AFLAS.","authors":"Noriyuki Kasai","doi":"10.1186/s42826-022-00144-1","DOIUrl":"https://doi.org/10.1186/s42826-022-00144-1","url":null,"abstract":"<p><p>The Asian federation of laboratory animal science associations (AFLAS) was established on November 29, 2003, and will celebrate its 20th anniversary in 2023. During this time, the number of AFLAS member associations and societies increased from six founders to eleven, and eight AFLAS congresses and 19 council meetings were held. In addition, the education and training system of laboratory animal science and technology funding program to support the activities of AFLAS member associations or societies started in 2015. Unfortunately, the COVID-19 pandemic had a great impact on the activities of AFLAS, and the 10th Congress which was scheduled to be held in Thailand in 2021 had to be canceled. AFLAS must have its members work together to overcome this difficult situation and further develop.</p>","PeriodicalId":17993,"journal":{"name":"Laboratory Animal Research","volume":"38 1","pages":"34"},"PeriodicalIF":2.9,"publicationDate":"2022-11-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9700945/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10327947","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Establishment of a novel experimental model of infected anal fistula in rat","authors":"Meng Zhao, Aitong Wang, Leisheng Zhang, Hao Yu","doi":"10.1186/s42826-022-00125-4","DOIUrl":"https://doi.org/10.1186/s42826-022-00125-4","url":null,"abstract":"","PeriodicalId":17993,"journal":{"name":"Laboratory Animal Research","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2022-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46036432","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Application time and persistence of transcranial direct current stimulation (tDCS) against neuronal death resulting from transient cerebral ischemia.","authors":"Jong-Hun Lee,&nbsp;Bo Hyun Jung,&nbsp;Ki-Yeon Yoo","doi":"10.1186/s42826-022-00121-8","DOIUrl":"https://doi.org/10.1186/s42826-022-00121-8","url":null,"abstract":"<p><strong>Background: </strong>Transcranial direct current stimulation (tDCS) has been studied as a tool to stimulate the functional recovery of neurons after stroke. Although this device has recently begun to be utilized for providing neuroprotection in stroke, research on its application conditions is lacking. This study aimed to examine the effects of various tDCS application conditions on cerebral ischemia. Ischemia was induced for 5 min in a gerbil model. The application of tDCS comprised a 20 min stimulation-20 min rest-20 min stimulation protocol, which was implemented simultaneously with the induction of cerebral ischemia. Application time of the tDCS effect on ischemia was confirmed by sampling brain tissues after stimulation using 0.2 mA tDCS at 0, 5, 10 and 60 min after ischemia.</p><p><strong>Results: </strong>Persistence of the tDCS effect on ischemia was confirmed by sampling brain tissues 5, 7, and 10 days post stimulation, with 0.2 mA tDCS after ischemia. Furthermore, the tissues were stained with cresyl violet and Fluoro-Jade C so as to determine the reduction in neuronal death under all application conditions.</p><p><strong>Conclusions: </strong>The application of tDCS can be used as a useful intervention for acute phase stroke due to its sustained neuroprotective effect.</p>","PeriodicalId":17993,"journal":{"name":"Laboratory Animal Research","volume":"38 1","pages":"12"},"PeriodicalIF":2.9,"publicationDate":"2022-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9082879/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10250951","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficacy of instillation of MB49 cells and thermoreversible polymeric gel in urothelial bladder carcinoma immunization","authors":"Jhonne Pedro Pedotte Santana, P. Marcato, T. Massaro, Naiane Lima Godoy, F. F. Anibal, R. Borra","doi":"10.1186/s42826-022-00122-7","DOIUrl":"https://doi.org/10.1186/s42826-022-00122-7","url":null,"abstract":"","PeriodicalId":17993,"journal":{"name":"Laboratory Animal Research","volume":"38 1","pages":""},"PeriodicalIF":2.9,"publicationDate":"2022-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"65797535","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}