Ulyanovsk Medico-biological Journal最新文献

{"title":"VARIANTS OF VENOUS SINUS STRUCTURE IN PATIENTS WITH CEREBRAL VENOUS THROMBOSIS","authors":"A. I. Sitnikova, L. A. Belova, V. V. Mashin, L. Matveeva, Dmitriy Vyacheslavovich Belov","doi":"10.34014/2227-1848-2022-4-19-29","DOIUrl":"https://doi.org/10.34014/2227-1848-2022-4-19-29","url":null,"abstract":"The aim of the paper is to study venous sinus structure in patients with cerebral venous thrombosis (CVT) depending on constitutional venous insufficiency (CVI). \u0000Materials and methods. The study included 50 CVT patients aged 25–77 years (mean age 52.5±14): 15 men (25.6 %) aged 46–75 years (mean age 54±12) and 35 women (74.4 %) aged 25–77 years (mean age 53.5±14). \u0000The authors assessed patients’ complaints, anamnesis, neurological status and assigned CVI clinical criteria. All patients underwent magnetic resonance venography. \u0000Results. In the study, transverse sinus CVT (24 cases (48.0 %)) and a combination of thrombosis of several localizations (19 cases (38.0 %)) were observed more often. Cavernous, superior sagittal and sigmoid sinus thromboses were observed in 2 (4.0 %), 2 (4.0 %) and 3 (6.0 %) cases, respectively. Left-sided CVT was observed more often compared with right-sided CVT (36 cases (72.0 %)) (p<0.05). \u0000Brain venous system varies greatly. Sinuses can be symmetrical or have anatomical structure in the form of hypoplasia. CVT patients demonstrated sinus hypoplasia more often (40 cases (71.4 %)) (p<0.05). At the same time, it was detected only in transverse and sigmoid sinuses, left-dominated (29 cases (72.5 %)). Cavernous sinuses had a symmetrical structure (p<0.05). \u0000CVI, manifested by clinical signs of impaired venous outflow, was more often in CVT patients with sinus hypoplasia (30 cases (75.0 %), compared with CVT patients without sinus hypoplasia (p<0.05).","PeriodicalId":177722,"journal":{"name":"Ulyanovsk Medico-biological Journal","volume":"70 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128514403","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"QUALITY OF LIFE IN PATIENTS WITH CORONARY HEART DISEASE AND BRONCHIAL ASTHMA","authors":"Elena Vladimirovna Noskova, Z. G. Simonova","doi":"10.34014/2227-1848-2022-4-30-37","DOIUrl":"https://doi.org/10.34014/2227-1848-2022-4-30-37","url":null,"abstract":"The purpose of the paper is to estimate the quality of life in patients with coronary heart disease (CHD) and bronchial asthma, taking gender into account. \u0000Materials and methods. An open prospective clinical study included 60 patients with stable angina (Grade I-III) and bronchial asthma. The patients were divided into two groups according to their gender: group I consisted of 32 men, group II included 28 women. CHD was detected by diagnostic search algorithms (recommendations of the Russian Society of Cardiology for stable coronary heart disease, 2020). The results of non-invasive and invasive tests and the clinical features of a disease were also taken into account. Patients’ quality of life was estimated by SF-36 questionnaire. The authors used statistical analysis to process the obtained data. \u0000Results. Psychological and mental components of health were calculated by SF-36 questionnaire. Psychological distress was observed in both study groups equifrequently. Women’s physical activity was almost two times lower than that in men. Thus, health problems limited women’s daily activities more often. \u0000Conclusion. Physical health in patients with stable angina and bronchial asthma, assessed by SF-36 questionnaire, is significantly lower in women compared to men. At the same time, psychological distress, manifested by anxiety or depression, is observed in men and women equifrequently.","PeriodicalId":177722,"journal":{"name":"Ulyanovsk Medico-biological Journal","volume":"88 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123330244","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"EXPRESSION OF H/ACA snoRNA IN CELL LINES WITH CHROMOSOMAL ABNORMALITIES AFTER IRRADIATION","authors":"E. Rastorgueva, E. Pogodina, E. Yurova, E. Beloborodov, D. Sugak, Y. Saenko, A. N. Fomin","doi":"10.34014/2227-1848-2022-4-149-159","DOIUrl":"https://doi.org/10.34014/2227-1848-2022-4-149-159","url":null,"abstract":"The H/ACA snoRNA family is involved in pseudouridine biogenesis. It prevents genetic changes in cells and makes them more stable due to ribosomal RNA characteristics. Therefore, the study of H/ACA snoRNA expression in cell lines with chromosomal disorders after irradiation is of particular interest. \u0000The purpose of the study is to analyze the effect of chromosomal disorders on H/ACA snoRNA expression in radioresistant K562 and radiosensitive HL-60 cell lines after radiation exposure. \u0000Materials and Methods. K562 and HL-60 cell lines were exposed to radiation (4 Gy). H/ACA snoRNA expression was analyzed by NGS sequencing (1, 4, and 24 hours after irradiation). \u0000Results. The authors revealed differences in H/ACA snoRNA expression by chromosomes in the studied cell lines, as well as the impact of chromosomal abnormalities on H/ACA snoRNA expression after radiation exposure. Changes in the copy number of normal chromosomes lead to minor changes in H/ACA snoRNA expression. Marker chromosomes disrupt H/ACA snoRNA expression. Thus, is becomes impossible to use H/ACA snoRNAs located in abnormal chromosomes as radioresistance markers. Moreover, marker chromosomes decrease the number of H/ACA snoRNAs expressed in K562, despite the greater amount of genetic material.","PeriodicalId":177722,"journal":{"name":"Ulyanovsk Medico-biological Journal","volume":"7 7 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121187457","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ACUTE KIDNEY INJURY IN COVID-19 PATIENTS","authors":"E. Sakaeva, A. М. Shutov, E. V. Efremova, Irina Olegovna Popondopolo","doi":"10.34014/2227-1848-2022-4-49-57","DOIUrl":"https://doi.org/10.34014/2227-1848-2022-4-49-57","url":null,"abstract":"Acute kidney injury (AKI) often complicates the progression of COVID-19 and increases in-hospital mortality. \u0000The aim of the study is to analyze AKI frequency, the time of its development and the possibility of using the ratio blood urea nitrogen/blood creatinine (BUN/Cr) as a biomarker for AKI progression in COVID-19 patients. \u0000Materials and methods. The authors examined 329 patients hospitalized with COVID-19 (157 women (47.7 %) and 172 men (52.3 %), mean age 58.0±14.3 years). The follow-up period was 12 months. COVID-19 was confirmed by a PCR test. AKI frequency, severity and time of development were studied in all patients. Moreover, the authors calculated the ratio blood urea nitrogen/blood creatinine (BUN/Cr, mg/dl:mg/dl). \u0000Results. AKI was diagnosed in 70 patients (21.3 %), including 12 patients (17.1 %) with an increase in creatinine level after hospitalization (in-hospital AKI) and 58 patients (82.9 %) with a high creatinine level (pre-hospital AKI). AKI stage 1 was observed in 55 patients (78.6 %), stage 2 – in 11 patients (15.7 %), stage 3 – in 4 patients (5.7 %). In-hospital mortality in COVID-19 patients with AKI was 10 %, the relative mortality risk in COVID-19 patients with AKI was 5.3 (95 %, CI 1.7–16.1; p=0.01). In patients hospitalized with AKI, AUB/Cr>20 was observed on hospitalization in 16 patients (27.6 %). In patients with in-hospital AKI, AUB/Cr>20 was detected only in 1 person (8 %). \u0000Conclusion. One in four patients hospitalized with COVID-19 develop AKI, predominantly stage 1. AKI increases in-hospital mortality. In most patients, AKI develops before hospitalization. In 27.6 % of patients with pre-hospital AKI, AUB/Cr>20 on hospitalization, which indicates the prerenal nature of AKI and the importance of dehydration (hypovolemia) as a risk factor for AKI progression in COVID-19 patients.","PeriodicalId":177722,"journal":{"name":"Ulyanovsk Medico-biological Journal","volume":"8 1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128837894","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"LASER ADJUVANTS: KEY FEATURES AND SPECIFICITY","authors":"R. S. Zayneeva, A.K. Gil'mutdinova, I. Zolotovskiy, A. Khokhlova, V. A. Ribenek, T. Gening","doi":"10.34014/2227-1848-2022-4-93-108","DOIUrl":"https://doi.org/10.34014/2227-1848-2022-4-93-108","url":null,"abstract":"Increasing the vaccine effectiveness and the search for new adjuvants that directly influence immunocompetent cells and stimulate the development of a pronounced adaptive immune response remain significant problems for modern medicine. Currently, aluminum salts and other chemicals with certain side effects are used as adjuvants. Therefore, it is relevant to search for other methods to increase vaccine effectiveness while reducing its toxic effect on the patients. One of such methods is laser irradiation of the injection sites, which, among other things, makes it possible to reduce vaccine amount. \u0000The purpose of this review is to analyze publications on the use of laser to stimulate the immune response. Four different classes of lasers are known to systemically enhance the immune response to intradermal vaccination: pulsed lasers, continuous mode lasers, non-ablative fractional lasers, and ablative fractional lasers. Each laser vaccine adjuvant is characterized by radiation parameters, modes of action, and immunological adjuvant effects that differ significantly. The authors consider main classes of lasers used as immunological adjuvants. The specificity of each laser will help to choose the most effective option to achieve the clinical goal when using a particular vaccine.","PeriodicalId":177722,"journal":{"name":"Ulyanovsk Medico-biological Journal","volume":"411 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133384771","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"EFFECTIVENESS OF ADDITIONAL RESPIRATORY RESISTANCE FOR PHYSICAL FITNESS OF JUDO ATHLETES","authors":"Y. Byalovskiy, I. Rakitina","doi":"10.34014/2227-1848-2022-4-128-138","DOIUrl":"https://doi.org/10.34014/2227-1848-2022-4-128-138","url":null,"abstract":"The development and implementation of scientifically based methods for athletes’ functional status improvement is rather relevant. One of such methods implies additional respiratory resistance (ARR). \u0000The aim of the study is to determine how additional respiratory resistance influences physical fitness of judo athletes. \u0000Materials and Methods. The trial subjects (n=42) were divided into two groups. The main group (n=25) trained under ARR equal to 20 % Pmmax, where Pmmax is the maximum intraoral pressure, evaluated under complete mouth and nose overlap. Athletes trained under ARR once a day for 6 weeks. The training session lasted 25 minutes. The control group (n=27) trained without ARR. \u0000Results. By the 6th week of physical training under ARR, the athletes of the treatment group demonstrated significant increase in tidal volume (p<0.05), maximum intraoral pressure (p<0.05), and physical performance (p<0.05) compared to the control. Maximum oxygen consumption significantly increased (p<0.05) in the athletes of the treatment group. The most significant changes in speed-strength readiness (compared to the control) were noted in barbell squats (p<0.05), 60-meter and 100-meter dash (p<0.01). \u0000Conclusion. 6-week training session with ARR equal to 20% Pmmax has a positive effect on the physical fitness of judo athletes.","PeriodicalId":177722,"journal":{"name":"Ulyanovsk Medico-biological Journal","volume":"23 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115120388","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"IMPACT OF THE GENE EXPRESSION LEVEL AND INTERMOLECULAR INTERACTION NETWORKS ON RADIORESISTANCE OF TUMOR CELLS","authors":"E. Pogodina, E. Rastorgueva, E. Yurova, E. Beloborodov, D. Sugak, Y. Saenko, A. N. Fomin, Maksim Anatol'evich Volkov, B. M. Kostishko","doi":"10.34014/2227-1848-2022-3-142-155","DOIUrl":"https://doi.org/10.34014/2227-1848-2022-3-142-155","url":null,"abstract":"Despite its efficacy, radiation therapy faces the challenges connected with accelerated reproduction of tumor cells and radioresistance of malignant neoplasms. \u0000The aim of the study was to analyze the impact of the gene expression level and intermolecular interaction networks on the development of tumor cell radioresistance. \u0000Materials and Methods. The authors used 4 tumor cell lines: (K562, HCT-116p53 (+/+), HCT-116p53 (–/–), and Me45. To study the cell line transcriptome. Affymetrix high-density hybridization DNA chips (HGU133A series) were used. Bioinformatic analysis of gene expression dynamics was performed using the original Gene Selector program. Intermolecular interaction networks were studied using the STRING online system. \u0000Results. After exposure to ionizing radiation at a dose of 4 Gy, the expression level of DAAM1, IFNAR2, PALLD, and STK17A genes increases in K562 cell line and decreases in HCT-116p53 (+/+), HCT-116p53 (–/–) and Me45. Numerous protein complexes of the studied genes were found with STRING online system. Thus, DAAM1, IFNAR2, PALLD, and STK17A genes influence the activity of some particles in the network of intermolecular interactions. Selected DAAM1, IFNAR2, PALLD and STK17A genes and protein-protein complexes encoded by DAAM1, TNK2, PTBP2 and DVL2; IFNAR2, STAT2, IRF9, JAK1, GNB2L1 and IFNAR1; PALLD, LPP and ACTN2 genes can be used as potential targets. Their modulation can increase the response of malignant neoplasm cells to ionizing radiation.","PeriodicalId":177722,"journal":{"name":"Ulyanovsk Medico-biological Journal","volume":"258 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122673241","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"LEVELS OF CERTAIN HORMONES AND RECEPTORS IN TUMOR AND PERITUMORAL AREA IN BALB/C NUDE FEMALE MICE WITH MULTIPLE PRIMARY MALIGNANT PROCESS DEVELOPING ON THE BACKGROUND OF PRIMARY IMMUNODEFICIENCY","authors":"E. Frantsiyants, V. Bandovkina, I. Kaplieva, E. Surikova, S. Shlyk, Irina Valer'evna Neskubina, Y. Pogorelova, L. Trepitaki, I. Kotieva, K. Shumarin","doi":"10.34014/2227-1848-2022-3-129-141","DOIUrl":"https://doi.org/10.34014/2227-1848-2022-3-129-141","url":null,"abstract":"Primary immunodeficiency is one of the reasons for the development of primary multiple malignant tumors. \u0000The aim of the study is to examine the levels of certain hormones and their receptors in the tissue and peritumoral area of B16/F10 melanoma and Lewis lung carcinoma (LLC) in case of isolated and combined subcutaneous growth in female mice with T-cell immunodeficiency. \u0000Materials and Methods. BALB/c Nude mice were divided into groups: intact group; 2 control groups (Group 1 with B16/F10 subcutaneous inoculation, Group 2 with LLC subcutaneous inoculation); main group (animals with LLC+B16/F10 inoculation). The ELISA method was used to determine the levels of free testosterone (Tf.), estrone (E1), prolactin (PRL), estrogen receptors (REα and REβ), androgen receptors (RA) and progesterone receptors (RP4) (Cassabio, China). Statistical processing of the obtained results was carried out on a personal computer using STATISTICA 10.0, parametric Student's test and nonparametric Wilcoxon-Mann-Whitney test. \u0000Results. In the main group, life expectancy reduced due to melanoma growth by 1.8 times and LLC decrease by 2.3 times. In animals with LLC+B16/F10, compared to those with an only one tumor growth variant, estrone level in tumors decreased, but free testosterone level increased. Melanoma growth in animals with LLC+B16/F10 was accompanied by an increase in prolactin level and some sex steroid receptors in the tumor tissue, its peritumoral area, and skin not affected by the malignant process. A decrease of Lewis carcinoma in females of the main group was accompanied by a decrease of all the studied receptors and hormones both in the tumor and its peritumoral area. \u0000Conclusions. Melanoma growth is probably associated with a high prolactin level and sex steroid receptors in unaffected skin, being a source of melanocytes, the cells from which this tumor develops.","PeriodicalId":177722,"journal":{"name":"Ulyanovsk Medico-biological Journal","volume":"84 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131381867","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"RECONSTRUCTION OF SCHIZOPHRENIA GENE NETWORK IN SEARCH FOR TARGET GENES","authors":"Anastasiya Yur'evna Dokhoyan, Maksim Vital'evich Glushchenko, Y. L. Orlov","doi":"10.34014/2227-1848-2022-3-6-22","DOIUrl":"https://doi.org/10.34014/2227-1848-2022-3-6-22","url":null,"abstract":"Nowadays, schizophrenia is a poorly understood disease with a variety of symptoms attributed to other malconditions, and controversial diagnosis without well-defined treatment. Target therapy implies disease gene network reconstruction, gene clustering, identification of gene ontology categories and genes with the largest number of network contacts. \u0000The aim of the study is to analyze schizophrenia-associated genes, determine their position in the gene network, establish their correlation, identify key genes related to the disease, and evaluate them as target genes for drug therapy. \u0000Materials and Methods. The authors analyzed currently relevant data on schizophrenia using such online databases as OMIM, PANTHER, DAVID, GeneMANIA, STRING-DB, and GeneCards. They calculated categories of gene ontologies for 200 genes, such as biological processes, molecular functions and cellular compartments that reflect schizophrenia impact on the transmission of neuronal impulses. The authors also visualized and built gene networks containing the identified key objects and their interaction, identified the most relevant schizophrenia genes (COMT, DISC1, HTR2A, NRXN1) and a strongly connected cluster, including such genes as BDNF, SLC6A4, HTR2A, HTR2C, CHRM1, SRC, AKT, YWHAE, DISC1, DRD2, COMT, NDEL1, NOS1, CAMK28, etc. \u0000Results. The biological interpretation of the results obtained is still a great challenge, since schizophrenia is a genetically complex disease with numerous causes and triggering events. Analysis of schizophrenia-associated genes, and identification of their position in the gene network (connectivity) makes it possible to find out their interaction, determine the key genes of the disease, and evaluate their prospects as target genes for drug therapy.","PeriodicalId":177722,"journal":{"name":"Ulyanovsk Medico-biological Journal","volume":"62 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125702030","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"EFFECTIVENESS OF EVALUATION OF APC, GSTP1 AND RASSF1A METHYLATION LEVEL AS A PROSTATE CANCER MARKER","authors":"I. Aboyan, E. N. Fedotova, A. Maksimov, E. F. Komarova","doi":"10.34014/2227-1848-2022-3-73-85","DOIUrl":"https://doi.org/10.34014/2227-1848-2022-3-73-85","url":null,"abstract":"Prostate cancer (PC) is one of the most common oncological diseases, ranking fourth in the global mortality structure. Due to the absence of clinical manifestations in the early stages, and poor methods of differential laboratory diagnostics, the search for sensitive minimally invasive prostate cancer (PC) markers remains relevant. \u0000The aim of the study was to analyze APC, GSTP1 and RASSF1A methylation levels in biological material in prostate pathologies and their effectiveness in PC detection. \u0000Materials and Methods. For molecular genetic study of APC, GSTP1 and RASFF1A methylation levels by molecular-specific PCR test, the authors used genomic DNA isolated from samples of post-massage urine, blood plasma and biopsy material from patients with PC (n=34) and benign prostatic hyperplasia (BPH) (n=27). The control group consisted of 20 men without any identified pathology. Analysis of molecular-specific PCR products was carried out by 2 % agarose gel electrophoresis. \u0000Results. The average APC, GSTP1, and RASFF1A methylation level was mainly noted in all types of biological material. There were statistically significant differences between groups with pancreatic pathologies, taking into account biological material. The evaluation of the odds ratio of PC detection showed that the hypermethylated APC in post-massage urine, GSTP1 in blood plasma, and RASFF1A in biopsy material increased the probability of PC detection by 2.5, 12.1, and 4.1 times, respectively. Low sensitivity (55.3 %) and high specificity (87 %) of PC diagnostics in terms of APC methylation in post-massage urine, GSTP1 in blood plasma, and RASFF1A in biopsy material were shown. With the combined use of the methylation gene status, the sensitivity was 65.2 %, and the specificity was 82.4 %. When the total prostate-specific antigen (PSA) value was added to the panel, the indices were 79.1 % and 82.9 %, respectively. \u0000Conclusion. APC methylation levels in post-massage urine, GSTP1 in blood plasma, and RASSF1A in biopsy can be considered as highly specific diagnostic PC markers. The combined use of these indicators increases the specificity of diagnosis in comparison with the initial PSA level. When included in the panel, the latter also increases the panel sensitivity for PC detection.","PeriodicalId":177722,"journal":{"name":"Ulyanovsk Medico-biological Journal","volume":"11 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122943420","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}