Journal of virological methods最新文献_第3页

Development and analytical validation of a quantitative PCR assay for the detection of Magellanic penguin herpesvirus 1 麦哲伦企鹅疱疹病毒1型定量PCR检测方法的建立及分析验证

IF 1.6 4区医学

Journal of virological methods Pub Date : 2025-08-28 DOI: 10.1016/j.jviromet.2025.115255

Maris J. Daleo , Laura A. Adamovicz , Karisa N. Tang , Matthew C. Allender

{"title":"Development and analytical validation of a quantitative PCR assay for the detection of Magellanic penguin herpesvirus 1","authors":"Maris J. Daleo , Laura A. Adamovicz , Karisa N. Tang , Matthew C. Allender","doi":"10.1016/j.jviromet.2025.115255","DOIUrl":"10.1016/j.jviromet.2025.115255","url":null,"abstract":"<div><div>Herpesviruses are associated with disease in several aquatic bird species, including penguins. Magellanic penguin herpesvirus 1 (MagHV1) was initially detected in 58.3 % of oiled Magellanic penguins (<em>Spheniscus magellanicus</em>) in South America presenting with respiratory distress characterized by a combination of necrohemorrhagic tracheitis, fibrinous air sacculitis, pneumonia, and death. Additional exploration is needed to understand how herpesviruses affect penguin health; however, there is currently a lack of rapid, sensitive, and specific methods for detecting and quantifying herpesvirus infections in this taxon. To address this problem, we developed a real-time quantitative PCR (qPCR) assay for the detection of MagHV1 in penguins. Using a commercial program, TaqMan-MGB primer-probes targeting the DNA polymerase gene were designed <em>in silico</em>. Inter- and intra-assay variability, dynamic range, limit of detection, and analytical specificity were assessed to validate the assay per MIQE guidelines. The resulting assay was highly specific for MagHV1, failing to amplify fifteen closely related avian herpesviruses. It performed with high efficiency (slope =-3.336, R<sup>2</sup> = 0.999, efficiency 99.40 %) and low inter- and intra-assay variability (coefficient of variation <u><</u> 1.67 % at all dilutions). Reaction efficiency was not impacted by the presence of penguin DNA from known-negative tracheal swabs. This qPCR assay has a linear range of detection from 10<sup>7</sup> to 10<sup>1</sup> viral copies per reaction and provides a valuable tool in the surveillance and characterization of MagHV1 epidemiology in penguins. This assay can further be used to detect asymptomatic birds and as an effective tool to monitor infectious individuals.</div></div>","PeriodicalId":17663,"journal":{"name":"Journal of virological methods","volume":"339 ","pages":"Article 115255"},"PeriodicalIF":1.6,"publicationDate":"2025-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144933144","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Detection and characterization of neonatal cytomegalovirus through nanopore sequencing using flongle flow cells: Pilot study in Philadelphia, Pennsylvania 利用flongle流式细胞通过纳米孔测序检测和表征新生儿巨细胞病毒：在宾夕法尼亚州费城的初步研究

IF 1.6 4区医学

Journal of virological methods Pub Date : 2025-08-24 DOI: 10.1016/j.jviromet.2025.115245

Alvaro Proaño , Joe Chan , Gabrielle C. Galchen , Mian Umair Ahsan , Robert H. Gilman , Kenneth P. Smith , Kai Wang , Dustin D. Flannery

{"title":"Detection and characterization of neonatal cytomegalovirus through nanopore sequencing using flongle flow cells: Pilot study in Philadelphia, Pennsylvania","authors":"Alvaro Proaño , Joe Chan , Gabrielle C. Galchen , Mian Umair Ahsan , Robert H. Gilman , Kenneth P. Smith , Kai Wang , Dustin D. Flannery","doi":"10.1016/j.jviromet.2025.115245","DOIUrl":"10.1016/j.jviromet.2025.115245","url":null,"abstract":"<div><h3>Background</h3><div>Cytomegalovirus (CMV) remains a significant infection in neonates and its early detection can aid with further treatment (antiviral, audiology). However, current diagnostics do not provide genetic information.</div></div><div><h3>Objective</h3><div>We explored the use of the portable and comprehensive sequencing method from Oxford Nanopore Technologies, utilizing low-cost Flongle flow cells to detect and perform sequence-level characterization of neonatal urine samples that tested positive for CMV by PCR.</div></div><div><h3>Study design</h3><div>We performed a pilot study based on a retrospective cohort study of neonates who were positive for CMV by PCR, who were admitted at two birth hospitals in Philadelphia, PA. We leveraged deep and long-read sequencing results to analyze the reads in two forms: by comparing them against a reference-based strain and by reconstructing the genome through de novo assembly with phylogenetic tree analysis.</div></div><div><h3>Results</h3><div>We assayed seven clinical samples, including a positive and negative control sample, from newborns ranging from 23 weeks' gestation to term, with testing performed for microcephaly, hearing test results, small gestational age, and thrombocytopenia. Each sample showed multiple differences compared to the reference strain, and the phylogenetic tree analysis of the de novo assembly depicted the genetic diversity of the samples.</div></div><div><h3>Conclusion</h3><div>This pilot study shows that nanopore sequencing with low-cost Flongle flow cells can detect and characterize CMV strains from clinical neonatal urine samples. This, coupled with current screening and diagnostic criteria, could further our genomic understanding of neonatal CMV, such as viral genome diversity, genotype-phenotype associations, and spread of strains.</div></div>","PeriodicalId":17663,"journal":{"name":"Journal of virological methods","volume":"339 ","pages":"Article 115245"},"PeriodicalIF":1.6,"publicationDate":"2025-08-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144903345","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative effects of three SARS-CoV-2 inactivation methods on cytokine detection using LEGENDplex™ bead-based immunoassays 三种SARS-CoV-2灭活方法对LEGENDplex™免疫检测细胞因子的比较效果

IF 1.6 4区医学

Journal of virological methods Pub Date : 2025-08-24 DOI: 10.1016/j.jviromet.2025.115244

Yifan Chen , Ting Zhang , Jie Zhang , Xixuan Dong , Lixiang Xue , Zhongnan Yin

{"title":"Comparative effects of three SARS-CoV-2 inactivation methods on cytokine detection using LEGENDplex™ bead-based immunoassays","authors":"Yifan Chen , Ting Zhang , Jie Zhang , Xixuan Dong , Lixiang Xue , Zhongnan Yin","doi":"10.1016/j.jviromet.2025.115244","DOIUrl":"10.1016/j.jviromet.2025.115244","url":null,"abstract":"<div><div>In virology-related studies, including severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), it is routine to inactivate body fluid samples carrying the virus to reduce the spread of the virus and guarantee the safety of biobankers and researchers. However, inactivation treatments may affect the molecular structure of proteins in biological samples, and it is necessary to select an inactivation method that has the least impact on the target molecule associated with protein detection techniques. Cytometric Bead Array (CBA), a novel and powerful technology, allows the simultaneous quantification of up to 10–30 different soluble proteins from one sample, with a particular focus on various cytokines and chemokines in human body fluids. But only a few studies have investigated the effect of inactivation methods on relevant assays. Therefore, this study aims to investigate various viral inactivation methods and evaluate their impact on LEGENDplex™ bead-based immunoassays. By detecting eight plasma samples and eight ascites samples, we assessed the impacts of heat denaturation, γ-irradiation, and paraformaldehyde (PFA) inactivation methods on certain protein components in plasma and ascites by LEGENDplex™ bead-based immunoassays. The results showed that heat treatment and γ-irradiation treatment had little effect on LEGENDplex™ bead-based immunoassays in both blood and ascites, while PFA treatment resulted in changes in multiple cytokines and chemokines. Among twenty-six cytokines and chemokines, perforin, I-TAC (CXCL11), Eotaxin (CCL11), and MIP-3α (CCL20) were more vulnerable to heat denaturation, γ-irradiation, and PFA treatments. To sum up, our findings provide evidence to inform the selection of optimal inactivation methods for reliable cytokine profiling in SARS-CoV-2-infected samples.</div></div>","PeriodicalId":17663,"journal":{"name":"Journal of virological methods","volume":"339 ","pages":"Article 115244"},"PeriodicalIF":1.6,"publicationDate":"2025-08-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144895297","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of optimized ELISA to detect anti-cytomegalovirus antibodies against immunogenic proteins 免疫原性蛋白抗巨细胞病毒抗体ELISA检测优化后的评价

IF 1.6 4区医学

Journal of virological methods Pub Date : 2025-08-23 DOI: 10.1016/j.jviromet.2025.115246

Abdulrahim R. Hakami , Fatimah A. Alshehri , Irfan Ahmad , Essa N. Shamakhi , Ahmed R. Gosady , Hussain M. Qusadi , Ali Y. Mujayridi , Alexander W. Tarr , Gasim Dobie

{"title":"Evaluation of optimized ELISA to detect anti-cytomegalovirus antibodies against immunogenic proteins","authors":"Abdulrahim R. Hakami , Fatimah A. Alshehri , Irfan Ahmad , Essa N. Shamakhi , Ahmed R. Gosady , Hussain M. Qusadi , Ali Y. Mujayridi , Alexander W. Tarr , Gasim Dobie","doi":"10.1016/j.jviromet.2025.115246","DOIUrl":"10.1016/j.jviromet.2025.115246","url":null,"abstract":"<div><div>Herpesviruses are common viruses that have infected the vast majority of people around the globe. Accurate diagnosis of these viruses using cost-effective methods is important, especially for asymptomatic cases. Diagnostic differentiation between naturally occurring antibodies and other antibodies is an area that requires investigation. The aim of this study was to optimize an immunoassay to detect anti-herpesvirus antibodies directed against immunodominant proteins. A total of 120 sera were collected at two stages (62 samples for the pilot experiment during coronavirus pandemic, and 58 samples were collected recently). An ELISA test was developed using cytomegalovirus phosphoprotein 150 (pp150) and herpes simplex virus 2 glycoprotein D (HSV-2 gD) to test the specificity of the test. The cutoff value was 0.218, which indicates low background noise. There was competitive binding of the CMVpp150 as competitor compared to HSV-2 gD. The test can be reliably used to specifically determine anti-viral immunodominant proteins. Future studies should explore the binding of antibodies to other specific viral antigens to further expand the scale of testing. Furthermore, studies should be performed to reproduce these results with comparability using commercial kits.</div></div>","PeriodicalId":17663,"journal":{"name":"Journal of virological methods","volume":"338 ","pages":"Article 115246"},"PeriodicalIF":1.6,"publicationDate":"2025-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144893579","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development and optimisation of influenza C and influenza D pseudotyped viruses 流感C型和流感D型假病毒的研制和优化

IF 1.6 4区医学

Journal of virological methods Pub Date : 2025-08-23 DOI: 10.1016/j.jviromet.2025.115243

Maria Giovanna Marotta , Martin Mayora Neto , Janet Daly , Meshach Maina , Pauline van Diemen , Helen Everett , Maria Stella Lucente , Michele Camero , Emanuele Montomoli , Claudia Maria Trombetta , Kelly da Costa , Nigel J. Temperton

{"title":"Development and optimisation of influenza C and influenza D pseudotyped viruses","authors":"Maria Giovanna Marotta , Martin Mayora Neto , Janet Daly , Meshach Maina , Pauline van Diemen , Helen Everett , Maria Stella Lucente , Michele Camero , Emanuele Montomoli , Claudia Maria Trombetta , Kelly da Costa , Nigel J. Temperton","doi":"10.1016/j.jviromet.2025.115243","DOIUrl":"10.1016/j.jviromet.2025.115243","url":null,"abstract":"<div><div>To facilitate the study of influenza C (ICV) and influenza D (IDV) viruses, we generated lentiviral pseudotyped viruses (PVs) expressing the hemagglutinin-esterase fusion (HEF) glycoprotein from ICV (C/Minnesota/33/2015) and IDV (D/Swine/Italy/199724–3/2015, D/Bovine/France/5920/2014, and D/Bovine/Ibaraki/7768/2016). The production of these PVs was optimised using different amount of human airway trypsin-like (HAT) protease to enhance HEF maturation, and the transduction efficiency was evaluated in multiple cell lines. Using these PVs, we established a pseudovirus-based microneutralisation (pMN) assay to measure neutralising antibody responses and adapted an esterase activity assay to evaluate PV. Specific antisera neutralised PVs but failed to inhibit esterase activity. These findings confirm that ICV and IDV PVs provide a scalable, sensitive, and safe tool for antiviral screening, and sero-epidemiological research.</div></div>","PeriodicalId":17663,"journal":{"name":"Journal of virological methods","volume":"339 ","pages":"Article 115243"},"PeriodicalIF":1.6,"publicationDate":"2025-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144916780","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of novel detection primers and screening for apple rubbery wood virus 1 and 2 in Germany 德国苹果胶木病毒1号和2号新检测引物的研制及筛选。

IF 1.6 4区医学

Journal of virological methods Pub Date : 2025-08-23 DOI: 10.1016/j.jviromet.2025.115241

Christine Seinsche, Constanze Berwarth, Marlen Brodbeck, Kerstin Zikeli, Andrej Vondran, Lennard Kleemann, Stefanie Thomas, Wilhelm Jelkmann

{"title":"Development of novel detection primers and screening for apple rubbery wood virus 1 and 2 in Germany","authors":"Christine Seinsche, Constanze Berwarth, Marlen Brodbeck, Kerstin Zikeli, Andrej Vondran, Lennard Kleemann, Stefanie Thomas, Wilhelm Jelkmann","doi":"10.1016/j.jviromet.2025.115241","DOIUrl":"10.1016/j.jviromet.2025.115241","url":null,"abstract":"<div><div>Apple rubbery wood disease is globally distributed and affects primarily apples and pears. A characteristic symptom of the disease, which is caused by the two viruses Apple rubbery wood virus (ARWV) 1 and 2 respectively, is enhanced branch and twig flexibility leading to reduced fruit yields. In this study, a highly sensitive RT-PCR detection system was established for reliable and specific detection of all ARWV-1 and ARWV-2 genomic segments, S, M and L, and their variants. Phloem was identified as convenient tissue for reliable ARWV detection. The detection system was applied to 1059 apple samples from German commercial orchards, experimental plots, and variety collections to investigate ARWV distribution across Germany. Overall, ARWV-1, ARWV-2, or mixed infections were identified in 14.2 % of the samples. Commercial orchard samples from thirteen federal states were tested. In eleven federal states ARWV-1 infections ranging from 2.1 % to 16.7 % were detected, in five federal states the ARWV-2 infection status varied from 1.6 % to 12.4 % and in one federal state ARWV-1 and -2 mixed infections were observed. Within the commercial orchard sample set, 47 different apple varieties were tested. Several varieties, highly relevant for apple growers, show ARWV infection rates between 5 % and 10 %. This study provides the first comprehensive overview of ARWV occurrence in German apple orchards and underscores the importance of sensitive diagnostic tools for effective disease monitoring, analysis of planting material and future management strategies.</div></div>","PeriodicalId":17663,"journal":{"name":"Journal of virological methods","volume":"339 ","pages":"Article 115241"},"PeriodicalIF":1.6,"publicationDate":"2025-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144959158","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effective RNA extraction with readily available reagents: A comparative analysis of alternative protocols 有效的RNA提取与现成的试剂：替代方案的比较分析

IF 1.6 4区医学

Journal of virological methods Pub Date : 2025-08-19 DOI: 10.1016/j.jviromet.2025.115242

Sandra González G , Ana Dias , Rodrigo Reis , Shirley Villalba , Cynthia Vazquez , Marilda Mendonça Siqueira , Fernando do Couto Motta

引用次数: 0

The dynamics of common respiratory pathogens in southern china under the context of COVID-19 pandemic and prediction of mycoplasma pneumonia (2018–2023) 2019冠状病毒病大流行背景下华南地区常见呼吸道病原体动态及肺炎支原体预测（2018-2023）

IF 1.6 4区医学

Journal of virological methods Pub Date : 2025-08-16 DOI: 10.1016/j.jviromet.2025.115240

LinXiu Zeng , YangQianXi Wang , Bo Xiang , JinQiong Lin , WenChuang Zeng , JingYi Liang

{"title":"The dynamics of common respiratory pathogens in southern china under the context of COVID-19 pandemic and prediction of mycoplasma pneumonia (2018–2023)","authors":"LinXiu Zeng , YangQianXi Wang , Bo Xiang , JinQiong Lin , WenChuang Zeng , JingYi Liang","doi":"10.1016/j.jviromet.2025.115240","DOIUrl":"10.1016/j.jviromet.2025.115240","url":null,"abstract":"<div><div>This study investigates the epidemiological characteristics of common respiratory pathogens across three distinct periods: before, during and after the COVID-19 pandemic, We aimed to determine whether new trends in the transmission of respiratory pathogens would emerge as non-pharmaceutical intervention (NPIs) were gradually lifted. We analyzed the differences observed between the pre-epidemic, epidemic and post-epidemic periods. An autoregressive integrated moving average (ARIMA) time series model was employed to predict future trends of Mycoplasma pneumoniae (MP) infections. MP infections were most prevalent among adolescents, exhibiting a positive rate of 46.73 %, whereas RSV infections were observed primarily in neonates with a rate of 24.30 %, followed by infants at 14.74 %. Before the COVID-19 pandemic, MP was identified as the predominant pathogen. However, during the pandemic, there was a marked decline in the positive detection rates of both MP and Influenza A( IAV), while the positive rate of respiratory syncytial virus (RSV)significantly increased. In period three, the trend of RSV remained relatively stable, and the levels of IAVwere consistent with those observed prior to the period one. Conversely, the proportion of MP rose substantially, with its detection rate rebounding to 29.00 %. The positive detection rates for Influenza B (IBV) and adenovirus (ADV) remained largely stable throughout the three periods. During the three periods, a shift in the age distribution of MP infections was noted, with the majority of cases occurring in the 3–17 year age group following the epidemic. Furthermore, according to the predictions of the Autoregressive Integrated Moving Average (ARIMA) model, a decline in the trend of MP epidemic is anticipated in the forthcoming few months.</div></div>","PeriodicalId":17663,"journal":{"name":"Journal of virological methods","volume":"338 ","pages":"Article 115240"},"PeriodicalIF":1.6,"publicationDate":"2025-08-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144873859","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of Nano-qPCR assay for the diagnosis of Infectious Bovine Rhinotracheitis Virus 牛传染性鼻气管炎病毒纳米qpcr检测方法的建立。

IF 1.6 4区医学

Journal of virological methods Pub Date : 2025-08-14 DOI: 10.1016/j.jviromet.2025.115239

Lin Wang, Peili Hou, Hongmei Wang, Hongbin He

引用次数: 0

Development of a lateral flow immunoassay for rapid detection of piper yellow mottle and cucumber mosaic viruses in black pepper 建立快速检测黑胡椒黄斑驳病毒和黄瓜花叶病毒的横向流动免疫分析法。

IF 1.6 4区医学

Journal of virological methods Pub Date : 2025-08-07 DOI: 10.1016/j.jviromet.2025.115238

M. Greeshma , A.I. Bhat , A. Jeevalatha , P. Malavika

{"title":"Development of a lateral flow immunoassay for rapid detection of piper yellow mottle and cucumber mosaic viruses in black pepper","authors":"M. Greeshma , A.I. Bhat , A. Jeevalatha , P. Malavika","doi":"10.1016/j.jviromet.2025.115238","DOIUrl":"10.1016/j.jviromet.2025.115238","url":null,"abstract":"<div><div>Piper yellow mottle virus (PYMoV) and cucumber mosaic virus (CMV) are major viral pathogens causing stunt disease in black pepper and related Piper species. As no chemical control is available, early and accurate detection is essential to prevent virus spread through vegetative propagation. Existing PCR-based assays are effective but often time-consuming, expensive, and reliant on specialized equipment. This study reports the development of a simple, rapid, and cost-effective lateral flow immunoassay (LFIA) for on-site detection of PYMoV and CMV infecting black pepper. Polyclonal antiserum was produced by immunizing rabbits with <em>in vitro</em> expressed coat proteins of both viruses, and its specificity was confirmed by western blotting. Purified immunoglobulin G (IgG) was used to prepare gold nanoparticle (GNP)-IgG conjugates. LFIA strips were assembled by optimizing sample pads, conjugate release pads, nitrocellulose membranes (10 µm pore size), test lines (0.75 mg/ml anti-PYMoV and anti-CMV IgG), control line (1 mg/ml goat anti-rabbit IgG), and absorbent pads. The optimized LFIA accurately detected PYMoV and CMV in virus-infected black pepper plants. The limits of detection for anti-PYMoV and anti-CMV LFIA were 100 ng and 50 ng of their respective homologous antigens. The assays were further validated using field samples from multiple black pepper varieties and other <em>Piper</em> species, including <em>P. betle</em> and <em>P. longum</em>. This LFIA offers a practical tool for rapid, on-site diagnosis to support the management of viral diseases in black pepper cultivation.</div></div>","PeriodicalId":17663,"journal":{"name":"Journal of virological methods","volume":"338 ","pages":"Article 115238"},"PeriodicalIF":1.6,"publicationDate":"2025-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144812202","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0