Journal of virological methods最新文献

{"title":"Molecular evolutionary insights into the host-virus relationship between Marburg Virus and its bat reservoir","authors":"Nikita Kar ,&nbsp;Srinjay Kumar Bharadaj ,&nbsp;Stella Bharadaj ,&nbsp;Supriyo Chakraborty","doi":"10.1016/j.jviromet.2026.115377","DOIUrl":"10.1016/j.jviromet.2026.115377","url":null,"abstract":"<div><div>Bats, often silent carriers of lethal pathogens, serve as natural reservoirs for numerous viruses capable of crossing species barriers with devastating effects. Among them, <em>Rousettus aegyptiacus</em> (<em>R. aegyptiacus</em>) has emerged as a pivotal species in understanding viral spillovers. This fruit bat hosts the Marburg virus (MARV), supporting its replication and occasional transmission to humans, where it causes fatal hemorrhagic fever. However, a striking paradox remains while MARV infection is deadly in humans, <em>R. aegyptiacus</em> remains asymptomatic. Driven by this contrast, our study explores the codon usage patterns to reveal evolutionary mechanisms underlying viral adaptation and host tolerance. Far from being a passive carrier, <em>R. aegyptiacus</em> represents an insightful model for antiviral research. Our analysis revealed distinct codon usage patterns in MARV, indicating fine-tuned evolutionary alignment with the host’s codon preferences. MARV exhibited low codon bias, suggesting broad compatibility with the host’s translational machinery. Further, COA and PR2 analyses suggested that ecological factors shape codon selection in both virus and host. RNA modification analysis revealed frequent cytosine-to-thymine transitions, implying conserved mutational pressures or host-driven editing. Variations in RCDI values indicated deoptimized codon usage.</div></div>","PeriodicalId":17663,"journal":{"name":"Journal of virological methods","volume":"343 ","pages":"Article 115377"},"PeriodicalIF":1.6,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146776501","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrated validation of internally controlled one-step multiplex real-time RT-PCR for dengue virus serotyping and monoplex assay for quantification","authors":"Le Thi Phuong Thuy ,&nbsp;Vi Thuy Tran ,&nbsp;Huynh Le Anh Huy ,&nbsp;Tran Thai Hung ,&nbsp;Nguyet Nguyen Minh ,&nbsp;Ho Quang Chanh ,&nbsp;Tran Bang Huyen ,&nbsp;Nguyen Lam Vuong ,&nbsp;Cameron P. Simmons ,&nbsp;Duong Thi Hue Kien ,&nbsp;Sophie Yacoub","doi":"10.1016/j.jviromet.2026.115381","DOIUrl":"10.1016/j.jviromet.2026.115381","url":null,"abstract":"<div><div>Dengue is a major global health challenge, yet no commercially validated molecular kits currently provide both serotyping and viral load quantification in a single integrated format. To address this gap, we developed and internally validated a one-step multiplex real-time RT-PCR assay that simultaneously detects all four dengue virus (DENV) serotypes in a single reaction (5-PLEX), together with a complementary monoplex system for quantitative viral load measurement. The 5-PLEX assay demonstrated high specificity and reliable serotyping performance during acute and early critical phases of dengue infection (day of illness ≤4). Monoplex assays for each serotype showed strong linearity, precision, and low analytical limits of detection (3–10 copies/reaction), enabling accurate quantification. Based on these findings, we propose a diagnostic framework: 5-PLEX for samples collected within the first four days of illness, the previously validated 3-PLEX assay for samples collected from day 5 of illness onward, and monoplex assays for serotype-specific viral load measurement. Internal and external process controls were incorporated to support accuracy and reproducibility within the validated framework and to facilitate future multi-laboratory evaluation. This harmonized is designed to facilitate standardized implementation across laboratories and supports improved inter-laboratory consistency in clinical diagnostics, genomic surveillance, therapeutic trials, and pathogenesis research.</div></div>","PeriodicalId":17663,"journal":{"name":"Journal of virological methods","volume":"343 ","pages":"Article 115381"},"PeriodicalIF":1.6,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147355066","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of a native-antigen Western blot using ZZ-R 127–derived FMDV non-structural proteins for qualitative serology to inform DIVA workflows","authors":"Fuat Özyörük ,&nbsp;Ünal Parlak ,&nbsp;Can Çokçalışkan ,&nbsp;Santina Grazioli ,&nbsp;Yılmaz Akça","doi":"10.1016/j.jviromet.2026.115362","DOIUrl":"10.1016/j.jviromet.2026.115362","url":null,"abstract":"<div><div>This study describes the development of a Western blot (WB) assay employing native non-structural proteins (NSPs) from foot-and-mouth disease virus (FMDV)–infected ZZ-R 127 cell lysates to support confirmatory serodiagnosis within DIVA (differentiation of infected from vaccinated animals) frameworks. Native antigens were generated from six FMDV isolates representing serotypes A, O, and Asia1 and were separated by denaturing SDS–PAGE. Antigen identity was verified by co-migration with recombinant counterparts and detection with monoclonal antibodies (MAbs). Using one NSP-positive and one NSP-negative polyclonal bovine serum, the assay demonstrated consistent identification of 2C, 3D, and an ∼25.8 kDa 3AB-consistent precursor as the most reliable diagnostic targets. While MAb profiling confirmed the presence of 3C in the lysates, it showed no reactivity with the polyclonal serum, indicating limited utility for serology. Pixel-based quantification revealed predictable dilution-dependent signal behavior within gels (<em>R</em>^<em>2</em> &gt; 0.91 on log-log plots), though inter-gel variability was substantial, favoring qualitative over quantitative application. Absence of signals in mock-infected cell lysates and in the negative serum supported specificity. By preserving the native processing context of immunodominant NSPs, this platform is well-suited as a second-tier tool to adjudicate equivocal ELISA results in vaccinated populations.</div></div>","PeriodicalId":17663,"journal":{"name":"Journal of virological methods","volume":"342 ","pages":"Article 115362"},"PeriodicalIF":1.6,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146142872","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Inactivation of infectious virus samples through UV irradiation for downstream analysis","authors":"Hugues Graf,&nbsp;Sébastien Faure,&nbsp;Maelle Mellano ,&nbsp;Stéphanie Baron,&nbsp;Cynthia Chapel,&nbsp;Fanny Savoye,&nbsp;Eric Richier","doi":"10.1016/j.jviromet.2026.115354","DOIUrl":"10.1016/j.jviromet.2026.115354","url":null,"abstract":"<div><div>Downstream analysis of infectious samples often needs to be performed outside a containment area, requiring prior inactivation before transfer. The inactivation of adenovirus, reovirus, and rabies virus by ultraviolet radiation at 254 nm (UV-C) was investigated with the objective of preserving the bacterial endotoxin test (BET) performance in irradiated samples. Virus samples containing endotoxins were exposed to UV-C doses ranging from 1 to 9 J/cm². A treatment of 5 J/cm² (2 × 2.5 J/cm²) applied to 1 mL volumes resulted in complete viral inactivation and acceptable endotoxin recovery. Samples containing cell culture media required 10-fold dilution prior to UV irradiation to overcome UV light attenuation and achieve complete virus inactivation. UV radiation had minimal impact on endotoxins, with recovery rates around 100 % compared to non-irradiated controls, regardless of UV dose and sample volume. The development of optimized UV-C irradiation conditions enabled the identification of a dose interval that ensures effective viral inactivation while maintaining BET specificity and applicability. The irradiation process is intended for industrial implementation on rabies vaccine samples, allowing testing outside containment areas and ensuring that biological samples are safely inactivated, without residual biological risk.</div></div>","PeriodicalId":17663,"journal":{"name":"Journal of virological methods","volume":"342 ","pages":"Article 115354"},"PeriodicalIF":1.6,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146046887","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence and ribavirin-mediated elimination of Morchella importuna endornavirus 1 (MiEV1) in cultivated Morchella species in China","authors":"Zhifeng Zhang ,&nbsp;Yingjun Zhang ,&nbsp;Zengqiang Shang ,&nbsp;Xiaoli Wang ,&nbsp;Yankui Li ,&nbsp;Zhaoyu Wang ,&nbsp;Longbo Zhao ,&nbsp;Ziyan Peng","doi":"10.1016/j.jviromet.2026.115349","DOIUrl":"10.1016/j.jviromet.2026.115349","url":null,"abstract":"<div><div>Fungal viruses compromise the cultivation of <em>Morchella</em>. We surveyed five mycoviruses (Morchella importuna endornavirus 1 [MiEV1], Morchella importuna endornavirus 2 [MiEV2], Morchella importuna endornavirus 3 [MiEV3], Morchella esculenta fusarivirus 1 [MeFV1], and Morchella esculenta fusarivirus 2 [MeFV2]) in 24 cultivated strains (8 <em>M. importuna</em>, 5 <em>M. septimelata</em>, and 11 <em>M. sextelata</em>) collected across 11 provinces in China and evaluated candidate antiviral agents. Only MiEV1 was detected, with a high incidence of 79.2 % (19/24) across all three <em>Morchella</em> species. Among seven tested chemicals (ampicillin, azithromycin, kanamycin, rifampicin, roxithromycin, acyclovir hydrochloride, and ribavirin), only ribavirin significantly inhibited MiEV1 replication. Successive ribavirin treatments eliminated MiEV1 from a representative <em>M. sextelata</em> strain (M11), with the virus-free status remaining stable after multiple subcultures on drug-free medium. Elimination of MiEV1 also significantly enhanced mycelial growth rate and biomass, and led to enhanced mycelial pigmentation, suggesting a potential negative impact of MiEV1 on vegetative growth and pigmentation in <em>M. sextelata</em>. These findings demonstrate that MiEV1 is prevalent in cultivated <em>Morchella</em> and that ribavirin provides an effective strategy for generating stable virus-free strains, offering opportunities for mechanistic studies and industrial cultivation.</div></div>","PeriodicalId":17663,"journal":{"name":"Journal of virological methods","volume":"342 ","pages":"Article 115349"},"PeriodicalIF":1.6,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145980344","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First report of laboratory-confirmed Zika virus infection in human from South Karnataka, India: A case study","authors":"Mallikarjun Koppad ,&nbsp;Santhosh Kogaluru Shivakumaraswamy ,&nbsp;Puneeth Thaduru Goolappa ,&nbsp;Maithili Thotadur Mahabaleswara ,&nbsp;Kasabi Gudadappa Siddappa ,&nbsp;Premalatha Dindare Eswarappa ,&nbsp;Siddesh Kaalavvala Channappa","doi":"10.1016/j.jviromet.2026.115357","DOIUrl":"10.1016/j.jviromet.2026.115357","url":null,"abstract":"<div><h3>Background</h3><div>Zika virus is a member of flaviviruses group and is primarily transmitted by infected mosquito. Most of the Zika virus infections are subclinical or self limiting illness like dengue fever, but certain cases are manifested with Guillain-Barre syndrome in adults and microcephaly in neonates from infected mothers. Since 2016, India has considerably reported the Zika virus infection from different states (Gujarat, Tamil Nadu, Madhya Pradesh, Rajasthan, Kerala, Maharashtra and Uttar Pradesh). Similarly there was a case suspecting arboviral infection in Shivamogga city in Mid June, 2024 and found positive for Zika virus.</div></div><div><h3>Method-</h3><div>A serum sample of patient with hemorrhagic fever syndrome from private super speciality hospital suspecting for Zika virus infection was collected and sent to our laboratory for diagnosis of Zika virus. The sample was screened by real time reverse transcription polymerase chain reaction (RT-PCR) and confirmed by nucleotide sequencing. Further, the sample was examined for presence of anti-Zika IgM antibody. Finding- The real time RT-PCR result revealed the presence Zika virus RNA in the sample (CT value- 27) and it was further confirmed by sequencing the partial envelope (E) gene. Additionally, the subsequent serum sample was positive for anti-Zika IgM antibody. The phylogenetic analysis evidenced that Zika virus identified in this study belongs to the Asian lineage and was homogenous to the previously reported strains from India.</div></div><div><h3>Interpretation</h3><div>The patient without any travel history admitted for hemorrhagic fever was found positive with Zika virus. This is the first case of laboratory-confirmed Zika virus infection in human from Shivamogga, South Karnataka. It was further confirmed by phylogenetic analysis of envelope coding region and detecting anti-Zika virus antibody. However further studies have to be transacted to know the source of infection and systematic community based surveillance is need of the hour for early detection of Zika virus transmission.</div></div>","PeriodicalId":17663,"journal":{"name":"Journal of virological methods","volume":"342 ","pages":"Article 115357"},"PeriodicalIF":1.6,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146105963","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Animal models illuminate dengue immunopathogenesis to guide vaccine and therapeutic developments","authors":"Muhammad Nadir Shabbir ,&nbsp;Thuy Linh Duong","doi":"10.1016/j.jviromet.2026.115350","DOIUrl":"10.1016/j.jviromet.2026.115350","url":null,"abstract":"<div><div>Dengue virus (DENV) infection affects over 390 million people annually, and its severe forms, including dengue hemorrhagic fever (DHF) and dengue shock syndrome (DSS), occur as a result of antibody-dependent enhancement (ADE), T-cell dysregulation, and a cytokine storm. This review integrates the results of human immunopathology with those reported by animal models, consolidating the findings in a systematic manner to validate these mechanisms. Research on AG129 interferon receptor-deficient mice consistently demonstrates ADE-mediated viremia and vascular leakage. However, humanized NSG-BLT mice recapitulate the classic antigenic sin of human T cells after heterotypic challenge with DENV. Cynomolgus macaque models are physiologically relevant for testing tetravalent vaccines under near-human circulatory conditions, and diet-induced obese mice are used to phenotype the effects of metabolic comorbidities on IL-1β-driven immunopathology. Data using standardized methods, including Evans blue extravasation assays, multiplex cytokine panels, and endpoints compliant with the ARRIVE 2.0 guidelines for animal research reporting, were associated with human clinical results. ARRIVE 2.0 is a set of guidelines to improve the transparency and reproducibility of animal research. It is worth noting that early experimental studies on the effectiveness and safety of Dengvaxia reported breakthrough viremia in macaque models. The protection provided by the monoclonal antibody C10 was shown to mitigate antibody-dependent enhancement (ADE) in murine models, though it is not associated with the Dengvaxia vaccine. The further development of these animal models will be necessary to expedite the safe development of next-generation dengue vaccines (TAK-003, TV003/TV005), antivirals (favipiravir, sofosbuvir), and immune modulators (IL-6 and TNF-α blockers) for use in endemic areas.</div></div>","PeriodicalId":17663,"journal":{"name":"Journal of virological methods","volume":"342 ","pages":"Article 115350"},"PeriodicalIF":1.6,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145980345","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of a rapid and portable detection method for canine distemper virus based on CRISPR-Cas13a","authors":"Yuxuan Jiang ,&nbsp;Zaixing Yang ,&nbsp;Jiamei Yang ,&nbsp;Yifan Li ,&nbsp;Jiaqi Liu ,&nbsp;Lili Zhao ,&nbsp;Junwei Ge","doi":"10.1016/j.jviromet.2026.115355","DOIUrl":"10.1016/j.jviromet.2026.115355","url":null,"abstract":"<div><div>Canine distemper virus (CDV) is a pathogenic microorganism that severely affects the respiratory, digestive, and nervous systems, causing multi-systemic symptoms. It infects nearly all terrestrial carnivores worldwide, particularly the <em>Canidae</em> and <em>Mustelidae</em> families, posing a serious threat to global socio-economic and public health security. Given the importance of etiological treatment and early diagnosis, developing novel detection methods with improved accuracy, rapidity, and user-friendliness is necessary for effective prevention and control of CDV infection. In this study, we established a novel testing method using recombinase-aid amplification (RAA) coupled with CRISPR-Cas13a and optimized the working concentration of CRISPR RNA (crRNA) and Cas13a for the lateral flow detection (LFD) of CDV. The RAA-CRISPR-Cas13a-LFD for CDV did not cross-react against other prevalent canine pathogens and the sensitivity can detect as little as 10² copies/μL of CDV cDNA plasmids. Additionally, combined with HUDSON this RAA-CRISPR-Cas13a-LFD method could be used to detect clinical samples within 1.5 h, with performance comparable to that of RT-PCR. The results for the RAA-CRISPR-Cas13a detection could be visualized using either fluorescence or lateral flow strips for in field-deployable viral diagnosis. Overall, our developed method showed good potential in point-of-care testing (POCT) to control and reduce the losses by CDV infection.</div></div>","PeriodicalId":17663,"journal":{"name":"Journal of virological methods","volume":"342 ","pages":"Article 115355"},"PeriodicalIF":1.6,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146078779","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of an ELISA using recombinant Dengue NS1 constructs to measure antibody titers and predict dengue disease outcome in a pediatric population in India","authors":"Bharti Pathak ,&nbsp;Aparna Chakravarty ,&nbsp;N.Veena Rani ,&nbsp;Anuja Krishnan","doi":"10.1016/j.jviromet.2026.115351","DOIUrl":"10.1016/j.jviromet.2026.115351","url":null,"abstract":"<div><div>The majority of the antibody response in dengue infection targets the structural envelope (E) and membrane (M) proteins of the virus. However, vaccine development is challenging because antibodies against the E protein often show cross-reactivity, leading to antibody-dependent enhancement (ADE) and worsening of disease severity. Non-structural protein 1 (NS1), secreted during the acute phase of infection, has been implicated as a pathogenic factor that contributes to vascular permeability and haemorrhage in severe dengue. In this study, we aimed to investigate the role of NS1 antibodies in disease manifestation. Full-length NS1, along with its N-terminal (NS1A) and C-terminal (NS1B) regions, was expressed and purified to delineate the antibody response against different domains. Serum samples from 102 dengue-positive paediatric patients were analysed for antibody titres against these NS1 constructs using ELISA. Overall higher antibody titres against all three NS1 recombinant proteins were observed in mild cases compared to severe ones with most significant towards NS1A.These findings suggest that antibodies directed against NS1, particularly its N-terminal region, may have a protective role and could serve as a potential correlate of protection in dengue infection.</div></div>","PeriodicalId":17663,"journal":{"name":"Journal of virological methods","volume":"342 ","pages":"Article 115351"},"PeriodicalIF":1.6,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146003247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of a rapid detection kit for chicken infectious anemia virus nucleic acid (RPA-LFD method)","authors":"Mei Tang ,&nbsp;Lun Shu ,&nbsp;Yifei Xiong ,&nbsp;Qinmin Zhu ,&nbsp;Yiwei Liu ,&nbsp;Minfan Huang ,&nbsp;Qiaoyang Teng ,&nbsp;Chunxiu Yuan ,&nbsp;Qinfang Liu ,&nbsp;Xue Pan ,&nbsp;Zhifei Zhang ,&nbsp;Bangfeng Xu ,&nbsp;Xiaona Shi ,&nbsp;Minghao Yan ,&nbsp;Dawei Yan ,&nbsp;Zejun Li","doi":"10.1016/j.jviromet.2026.115363","DOIUrl":"10.1016/j.jviromet.2026.115363","url":null,"abstract":"<div><div>Chicken infectious anemia virus (CIAV) is a major immunosuppressive pathogen that causes huge economic losses in the poultry industry. The virus persists in the host for an extended period, making it difficult to eliminate and highlighting the need for rapid and accurate detection methods. In this study, we developed a recombinase polymerase amplification-lateral flow dipstick (RPA-LFD) kit for on-site detection of CIAV. Optimal primers and probes were designed and screened based on conserved regions of the full-gene sequences of 251 CIAV strains, ensuring high specificity, with no cross-reactions with 12 other common avian viruses. It demonstrated high sensitivity with a minimum detection limit of 10 copies of CIAV DNA, achieving efficient detection in just 15 min. The kit also showed good repeatability and stability, with 100 % concordance with qPCR in detecting 50 field samples. The developed CIAV RPA-LFD kit provides a practical tool for the rapid detection of CIAV, suitable for both on-site and laboratory use.</div></div>","PeriodicalId":17663,"journal":{"name":"Journal of virological methods","volume":"342 ","pages":"Article 115363"},"PeriodicalIF":1.6,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146137571","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}