Journal of virological methods最新文献

{"title":"Genomic characterization of the Coxsackievirus A24 variant in the Acute Hemorrhagic Conjunctivitis outbreak (2023) in Islamabad, Pakistan through metagenomic next generation sequencing","authors":"Syed Adnan Haider ,&nbsp;Zunera Jamal ,&nbsp;Muhammad Ammar ,&nbsp;Rabia Hakim ,&nbsp;Babak Afrough ,&nbsp;Anila Kreku ,&nbsp;Leena Inamdar ,&nbsp;Muhammad Salman ,&nbsp;Massab Umair","doi":"10.1016/j.jviromet.2025.115213","DOIUrl":"10.1016/j.jviromet.2025.115213","url":null,"abstract":"<div><div>Pakistan experienced a significant outbreak of Acute Hemorrhagic Conjunctivitis (AHC) in 2023. To identify the cause, in the absence of targeted diagnostic tests, the National Institute of Health, Islamabad, studied 15 conjunctivitis patients from Islamabad in September 2023. Metagenomic Next Generation Sequencing (mNGS) was performed on 10 samples collected within 48 h of symptom onset. The human Coxsackievirus A24 variant (CV-A24v), genotype IV, was detected in three samples. Phylogenetic analysis showed ∼99 % similarity with recent strains from China and 94 % similarity with the 2015 France outbreak. Mutation analysis revealed mostly non-synonymous substitutions, particularly in the VP1 region (n = 14), and differences in 2 C and 3D regions of nonstructural proteins. Comparison with 2005 Pakistan outbreak sequences showed divergence in the VP1 region, with two distinct mutations (\"L16I\" and \"L25H\"), with L16I being a rare mutation observed only in strain from China and India in 2023. Structural modeling of VP1 proteins indicated conformational differences between the 2023 and 2005 strains, suggesting potential impacts on viral infectivity and immune escape. These findings indicate the reemergence of CV-A24v in Pakistan and highlight the importance of adaptable diagnostic strategies to respond to emerging infectious threats.</div></div>","PeriodicalId":17663,"journal":{"name":"Journal of virological methods","volume":"338 ","pages":"Article 115213"},"PeriodicalIF":2.2,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144501755","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of a novel plate reader-based antibody-dependent enhancement (ADE) assay for orthoflavivirus infections","authors":"Yannis Aklil ,&nbsp;Hiroto Takeuchi ,&nbsp;Gabriel Gonzalez ,&nbsp;Atsuko Inoue ,&nbsp;Kaito Maeda ,&nbsp;Shintaro Kobayashi ,&nbsp;Yukari Itakura ,&nbsp;Shinji Saito ,&nbsp;Anavaj Sakuntabhai ,&nbsp;Michihito Sasaki ,&nbsp;William W. Hall ,&nbsp;Hirofumi Sawa ,&nbsp;Yasuko Orba ,&nbsp;Koshiro Tabata","doi":"10.1016/j.jviromet.2025.115210","DOIUrl":"10.1016/j.jviromet.2025.115210","url":null,"abstract":"<div><div>Antibody-dependent enhancement (ADE) is one of the mechanisms associated with severe clinical outcomes in infections caused by certain viruses, including dengue virus (DENV). Several ADE assay systems have been established, including flow cytometric assays using live viruses, enzyme-linked immuno-sorbent assay (ELISA) for the detection of viral NS1, and luciferase reporter gene assays. Among these, the flow cytometric assay is the most commonly used to evaluate ADE activity; however, it has limitations such as high operational costs due to fixation and immunostaining procedures, as well as a long analysis time. Fluorescent protein-expressing single-round infectious particles (SRIPs) enables label-free detection of ADE activity, but the flow cytometric procedure still requires a long analysis time. In this study, to simplify and expedite the ADE assay using enhanced green fluorescent protein (EGFP)-expressing SRIPs, we developed a plate reader-based ADE assay as an alternative to the conventional flow cytometry-based method. To evaluate effectiveness of this assay, we measured ADE activities in K562 cells induced by pan-orthoflavivirus 4G2 and pan-dengue 4E11 monoclonal antibodies (mAbs) using both flow cytometric assays using live viruses and plate-reader-based EGFP-expressing SRIPs assays. The results showed strong correlations between the two different ADE assays with R² values of 0.92 for 4G2 mAb and 0.94 for 4E11 mAb (Pearson correlation coefficients). In summary, this newly established assay offers a high-throughput and cost-effective method for comprehensive characterization of the relationship between vaccine- or infection-induced antibodies and ADE in orthoflavivirus infections.</div></div>","PeriodicalId":17663,"journal":{"name":"Journal of virological methods","volume":"338 ","pages":"Article 115210"},"PeriodicalIF":2.2,"publicationDate":"2025-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144371303","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of the flinders technology associates cards for storage and molecular detection of Infectious hypodermal and hematopoietic necrosis virus (IHHNV) DNA","authors":"Xin-Yu Lian ,&nbsp;Xiu-Hua Wang ,&nbsp;Chen Li ,&nbsp;Qing-Li Zhang ,&nbsp;Ruo-Xuan Lu ,&nbsp;Zi-Yue Gou ,&nbsp;Hua Xu ,&nbsp;Mei-Feng Wang ,&nbsp;Peng Jia ,&nbsp;Bing Yang","doi":"10.1016/j.jviromet.2025.115209","DOIUrl":"10.1016/j.jviromet.2025.115209","url":null,"abstract":"<div><div>Finders Technology Associates (FTA) cards offer a streamlined solution for the storage, transportation, and extraction of samples. In this study, we assessed the diagnostic efficacy of IHHNV DNA sample storage on FTA cards following simulated transportation under varying conditions (-20°C, 4°C, 25°C, 37°C) over a 60d period. By comparing two elution methods, we determined that optimal results were achieved by performing three elutions with FTA purification reagent at room temperature, followed by two elutions with TE buffer, each lasting 5 min, with a 200 μL elution volume. After the final elution, the membrane was air-dried at room temperature for 1 h before real-time PCR analysis. Our findings revealed that there was no statistically significant difference in nucleic acid preservation with respect to storage temperature and time (<em>P</em> &gt; 0.05). This suggests that nucleic acids stored on FTA cards remained stable under all tested temperature conditions for a minimum of 2 months. For on-site IHHNV monitoring, we analyzed shrimp homogenate samples. Our results demonstrated that eluting and extracting nucleic acid directly from shrimp tissue using FTA cards yielded results comparable in magnitude to conventional reagent kits when analyzed using real-time PCR. This underscores the equivalence of these two methods for nucleic acid extraction. FTA cards prove to be an effective tool for storing and detecting IHHNV DNA samples, offering a convenient and environmentally friendly solution. This method can be routinely employed and easily transported via standard postal systems, making it particularly advantageous for cross-border transportation.</div></div>","PeriodicalId":17663,"journal":{"name":"Journal of virological methods","volume":"338 ","pages":"Article 115209"},"PeriodicalIF":2.2,"publicationDate":"2025-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144501754","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of virus analytical methods on the estimation of virus reductions by ultrafiltration","authors":"Kaitlyn J. Chung ,&nbsp;Abigail G. Albright ,&nbsp;Dave W. Goad ,&nbsp;Andrew E. Page ,&nbsp;Bianca M. Souza-Chaves ,&nbsp;Andrea Achilli ,&nbsp;Michael J. Hegetschweiler ,&nbsp;Lester M. Shulman ,&nbsp;Walter Q. Betancourt","doi":"10.1016/j.jviromet.2025.115208","DOIUrl":"10.1016/j.jviromet.2025.115208","url":null,"abstract":"<div><div>This study evaluated the influence of analytical methods on the quantitative estimation of viruses in recycled waters and their reductions by an ultrafiltration (UF) engineering-scale system. Adenoviruses, crAssphage, <em>Pepper mild mottle virus</em> (PMMoV), culturable male-specific and somatic coliphages selected through a comparative quantitative analysis were evaluated in UF feed and UF permeate using a combination of analytical methods for virus concentration and absolute quantification of virus genomes and infectious coliphages by digital PCR and plaque assays, respectively. Both methods of virus concentration, centrifugal ultrafiltration and the InnovaPrep CP Select™ Concentrating Pipette (CP), demonstrated similar performance for the recovery of viruses from UF feed and UF permeate, however the CP approach allowed more rapid sample filtration than centrifugal ultrafiltration. Procedures commonly applied for the detection of viruses in water generated different quantitative outcomes that were influenced by the type of virus and other natural sources of variation associated with UF feed and UF permeate water. These quantitative outcomes in virus measurements led to highly variable estimations of virus reductions ranging from no apparent reduction to a maximum of 2-log₁₀. These results indicate that more accurate estimations of virus levels and reductions in water purification processes require adjustments in analytical procedures under the wide variety of water characteristics associated with each stage of treatment. A thorough understanding of the interactions between structural features of natural occurring viruses in recycled waters and water quality characteristics is crucial for improving virus recoveries and for the assessment of membrane-based treatment systems as barriers against microbial targets of environmental and public health concern.</div></div>","PeriodicalId":17663,"journal":{"name":"Journal of virological methods","volume":"338 ","pages":"Article 115208"},"PeriodicalIF":2.2,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144330333","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Construction and application of infectious cDNA clone, subgenomic replicon and packaging system for Zika virus and Dengue virus","authors":"Yu He ,&nbsp;Yibin Tang ,&nbsp;Xiaoli Wang ,&nbsp;Zhen Wu ,&nbsp;Tao Wang ,&nbsp;Mingshu Wang ,&nbsp;Renyong Jia ,&nbsp;Dekang Zhu ,&nbsp;Mafeng Liu ,&nbsp;Xinxin Zhao ,&nbsp;Qiao Yang ,&nbsp;Ying Wu ,&nbsp;Shaqiu Zhang ,&nbsp;Juan Huang ,&nbsp;Bing Tian ,&nbsp;Xumin Ou ,&nbsp;Di Sun ,&nbsp;Anchun Cheng ,&nbsp;Shun Chen","doi":"10.1016/j.jviromet.2025.115207","DOIUrl":"10.1016/j.jviromet.2025.115207","url":null,"abstract":"<div><div>Flaviviruses pose a significant global health threat due to their rapid spread and potential to cause severe clinical manifestations. Comprehending the mechanisms of replication of these pathogens and developing effective antiviral strategies are essential for combating these pathogens. In the present study, full-length infectious cDNA clones were generated for Zika virus (ZIKV) and Dengue virus (DENV), respectively. Recombinant viruses were successfully produced by transfecting cDNA clone plasmids. Using the infectious clone, ZIKV and DENV subgenomic replicons were also generated, which lack the prM-E gene and instead expressing a luciferase or fluorescent marker (OXGFP or mCherry). The replicons exhibited efficient replication in BHK-21 cells. Through the utilization of ZIKV and DENV-2 replicons that express luciferase, three potential antiviral agents were identified. These agents demonstrated activity against DENV-2 and ZIKV, while not inducing significant cytotoxic effects. This demonstrates the significance of these replicons in the screening of antiviral agents. Moreover, DENV-2 and ZIKV single-round infectious particles (SRIPs) were produced by co-transfecting packaging plasmids and replicons into BHK-21 cells. The packaging assay demonstrated that flaviviruses can utilize the prM-E protein from other species to generate SRIPs. The specific binding of the nucleocapsid (NC) to the prM-E protein varies among different flaviviruses. The reverse genetics tools established in this study will facilitate research on DENV-2 and ZIKV virus replication and the development of antiviral medications targeting these two arboviruses.</div></div>","PeriodicalId":17663,"journal":{"name":"Journal of virological methods","volume":"338 ","pages":"Article 115207"},"PeriodicalIF":2.2,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144330332","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quantification of Zika virus using a colloidal gold nanoparticle-based immunosensor and Fourier-transform infrared spectroscopy","authors":"Mario Alberto Cuapa-González ,&nbsp;Gerardo Santos-López ,&nbsp;Hugo Martínez-Gutiérrez ,&nbsp;Zeus Saldaña-Ahuactzi ,&nbsp;Francisco Severiano-Carrillo ,&nbsp;Orlando Zaca-Morán ,&nbsp;Abdu Orduña-Díaz ,&nbsp;Marlon Rojas-López","doi":"10.1016/j.jviromet.2025.115206","DOIUrl":"10.1016/j.jviromet.2025.115206","url":null,"abstract":"<div><div>Zika virus (ZIKV) is an arthropod-borne virus that has spread worldwide and is the etiological agent of Zika fever, a disease of global concern distinguished by mild symptoms and recently linked to a congenital syndrome in neonates. Microcephaly, hydrocephalus, ocular deformations, and meningoencephalitis characterize this disease. The plaque assay is the gold standard used in scientific research laboratories to quantify the virus titer on a sample and assess their relationship with the infectious dosage. However, it requires costly infrastructure and specialized technical training. In this study, a nano-immunosensor based on gold nanoparticles was developed to detect Zika virus using FTIR spectroscopy. Three calibration curves were established to estimate ZIKV titers by measuring the intensity of absorption bands associated with functional groups in viral samples at different concentrations. The detection sensitivity ranged from 5 × 10⁴ to 5 × 10⁷ PFU/mL for the lipid group (C<img>O, 1750 cm⁻¹), 5 × 10 ³ to 5 × 10⁷ PFU/mL for the protein group (amide I, 1680 cm⁻¹), and 5 × 10⁵ to 2.2 × 10⁸ PFU/mL for the carbohydrate group (C-O, 860 cm⁻¹). The proposed method highlights the potential of the colloidal immunosensor for the quantification of the Zika virus.</div></div>","PeriodicalId":17663,"journal":{"name":"Journal of virological methods","volume":"338 ","pages":"Article 115206"},"PeriodicalIF":2.2,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144330464","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Considerations and limitations for establishing an Intact Proviral DNA Assay (IPDA) on a chip-based digital PCR system for HIV-1 reservoir quantification","authors":"Jasmin Tschumi ,&nbsp;Kathrin Neumann ,&nbsp;Dominique L. Braun ,&nbsp;Huldrych F. Günthard ,&nbsp;Karin J. Metzner ,&nbsp;the Swiss HIV Cohort Study","doi":"10.1016/j.jviromet.2025.115205","DOIUrl":"10.1016/j.jviromet.2025.115205","url":null,"abstract":"<div><div>The intact proviral DNA assay (IPDA) has become a gold standard for HIV-1 reservoir quantification in HIV-1 latency research, as well as in the evaluation of HIV-1 cure strategies. In this work, we adjusted the IPDA assay to a chip-based digital PCR (dPCR) system, established the use of CCR5 as a different reference gene, and evaluated the performance on cell lines, clinical samples from people with HIV (PWH) off and on antiretroviral therapy (ART), and different HIV-1 subtypes. Our adapted IPDA performs well on the chip-based dPCR system with no false positive intact HIV-1 in negative controls and with less hands-on time compared to droplet-based dPCR systems. Undetectable intact HIV-1 DNA is common in clinical samples on ART and correlated with total HIV-1 reservoir size and sample input concentration for HIV-1 subtype B samples. Performance on non-B subtypes varies depending on the subtype and should be improved with subtype specific primer/probe combinations.</div></div>","PeriodicalId":17663,"journal":{"name":"Journal of virological methods","volume":"338 ","pages":"Article 115205"},"PeriodicalIF":2.2,"publicationDate":"2025-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144330465","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"IC-ELISAs for the quantitative detection of monkeypox virus cross-immunogenic modified vaccinia virus Ankara antigens L1 and A33","authors":"Mingchan Liang ,&nbsp;Feixia Gao ,&nbsp;Min Liu ,&nbsp;Linya Zhang ,&nbsp;Yongshan Zheng ,&nbsp;Jinhui Miao ,&nbsp;Cheng He ,&nbsp;Zhewen Chen","doi":"10.1016/j.jviromet.2025.115204","DOIUrl":"10.1016/j.jviromet.2025.115204","url":null,"abstract":"<div><div>Vaccination is always the most effective approach to control and prevent monkeypox epidemics. The efficacy testing of vaccines is an important component of vaccine quality control. Based on the 3Rs principles, measuring the content of effective proteins in vaccines to evaluate vaccine efficacy is an excellent alternative to traditional <em>in vivo</em> assays. In this study, we reported two indirect competitive ELISA (IC-ELISA) methods based on the use of M1- and A35-specific antibodies that recognize the L1 and A33 proteins of the modified vaccinia virus Ankara (MVA) vaccine, which are cross-immunogenic to monkeypox virus (MPXV), respectively. M1-IC-ELISA was shown to be linear over the range of 31.25–2000 ng/mL with an LOD value of 48.36 ng/mL. A35-IC-ELISA was shown to be linear over the range of 3.90–1000 ng/mL with an LOD value of 3.74 ng/mL. These two methods were found to be specific, precise, accurate and robust in the quantification of cross-immunogenic L1 and A33 in final MVA vaccine and showed a strong correlation with viral titer, MPXV cross-antibody titer and MVA neutralizing antibody titer. The developed methods may be an alternative to the <em>in vivo</em> assay in quality control testing of MVA vaccine.</div></div>","PeriodicalId":17663,"journal":{"name":"Journal of virological methods","volume":"338 ","pages":"Article 115204"},"PeriodicalIF":2.2,"publicationDate":"2025-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144371302","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Design and feasibility of a novel personal protection device against airborne pathogens for everyday use","authors":"Gary Leschinsky ,&nbsp;W. Andrew Dexter ,&nbsp;Barbara Leschinsky ,&nbsp;Jeffery Trolinger","doi":"10.1016/j.jviromet.2025.115203","DOIUrl":"10.1016/j.jviromet.2025.115203","url":null,"abstract":"<div><div>Airborne viruses, such as SARS-CoV-2, influenza, and potentially emerging avian influenza, are a major cause of disease spread. Airborne disease outbreaks have increased by 35 times in the past 20 years. These viruses are particularly a concern for immunocompromised individuals worldwide. ActivAir is a patented novel reusable personal protection device designed to provide a pathogen-free personal air supply. Ambient air is passed under UVC light inside a small, enclosed chamber before being released as a gentle breeze over the face of the user. Feasibility tests with a prototype disinfection chamber of the device showed a 99.99 % efficiency in a single-pass deactivation of aerosolized Phi X 174 bacteriophage, and over 96 % efficiency in deactivating aerosolized MS2 bacteriophage, both are known surrogates for SARS-COV-2, influenza, and other airborne viruses. High-efficiency UVC LEDs consume low enough energy to make this battery-powered device wearable and easy to use, lasting several hours on a single charge. This device holds a promise of providing efficient everyday protection for immunocompromised patients today, as well as a new line of defense for everyone in case of a future pandemic.</div></div>","PeriodicalId":17663,"journal":{"name":"Journal of virological methods","volume":"338 ","pages":"Article 115203"},"PeriodicalIF":2.2,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144313605","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Illumina MiSeq and iSeq platforms yield comparable results for viral genomic sequencing","authors":"Darlene D. Wagner ,&nbsp;Grace Nabakooza ,&nbsp;Nehalraza Momin ,&nbsp;Rachel L. Marine","doi":"10.1016/j.jviromet.2025.115202","DOIUrl":"10.1016/j.jviromet.2025.115202","url":null,"abstract":"<div><h3>Summary</h3><div>Illumina MiSeq and iSeq are widely used short-read next-generation sequencing (NGS) platforms. The MiSeq instrument is specialized for mid-range throughput, while the iSeq instrument has lower throughput but is less expensive and simpler to operate. Several studies have compared Illumina platforms for sequencing of a variety of specimen types, but very few have quantified differences in sequencing quality, particularly for viruses. This study compared read quality, single nucleotide polymorphism (SNP) calling, and assembly metrics for SARS-CoV-2, norovirus, and poliovirus samples sequenced on both platforms. MiSeq and iSeq trimmed reads exhibited equivalent percentage of bases ≥ Q30 (% ≥ Q30) and equivalent reference mapping percentages. SNP concordance rates between the two platforms were 41 out of 43 (95.3 %) for SARS-CoV-2, 1628 out of 1633 (99.7 %) for norovirus, and 9 out of 11 (81.8 %) for poliovirus. Within each of the three virus groups, MiSeq and iSeq assemblies had equivalent N50 and maximum contig lengths. Despite platform-specific differences in the sequencing chemistry and flow cell design, MiSeq and iSeq offered comparable data quality, indicating that using either platform should produce concordant results for viral genomic sequencing.</div></div>","PeriodicalId":17663,"journal":{"name":"Journal of virological methods","volume":"338 ","pages":"Article 115202"},"PeriodicalIF":2.2,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144302403","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}