Journal of virological methods最新文献

Development of a rapid RPA-CRISPR/Cas12b assay for dual detection of Hantaan virus and Seoul virus. 双重检测汉滩病毒和首尔病毒的RPA-CRISPR/Cas12b快速检测方法的建立

IF 1.7 4区医学

Journal of virological methods Pub Date : 2026-10-17 DOI: 10.1016/j.jviromet.2026.115483

Guo Ruowen, Tang Haitao, Fu Hongkun, Wang Weilin, Yu Baosheng, Xiong Mengya, Xu Jia, Hu Chaojie, Chen Xiaoyu

{"title":"Development of a rapid RPA-CRISPR/Cas12b assay for dual detection of Hantaan virus and Seoul virus.","authors":"Guo Ruowen, Tang Haitao, Fu Hongkun, Wang Weilin, Yu Baosheng, Xiong Mengya, Xu Jia, Hu Chaojie, Chen Xiaoyu","doi":"10.1016/j.jviromet.2026.115483","DOIUrl":"10.1016/j.jviromet.2026.115483","url":null,"abstract":"<p><p>Hantaan virus (HTNV) and Seoul virus (SEOV) are the primary causative agents of hemorrhagic fever with renal syndrome (HFRS), presenting a persistent challenge to global public health. Prompt and precise diagnostic tools are essential for clinical investigation and the containment of HFRS outbreaks. Leveraging the programmable precision of CRISPR/Cas12b system, which has significantly advanced the field of molecular diagnostics, we developed a rapid, high-sensitivity assay for the dual detection of HTNV and SEOV. Each virus was detected in a separate, target-specific single-tube reaction. The assay combines recombinase polymerase amplification (RPA) with CRISPR/Cas12b-mediated fluorescence detection and uses virus-specific sgRNAs and RPA primers targeting conserved genomic regions. Using recombinant plasmid standards, both targets were detected at 1 × 10<sup>1</sup> copies/test in three technical replicates; this is a preliminary analytical detection threshold and does not incorporate extraction losses or matrix-associated inhibition. No cross-reactivity was observed within the evaluated four-organism non-target panel. In a preliminary feasibility assessment, all nine RT-qPCR-positive rodent samples (seven HTNV and two SEOV) were positive by the RPA-CRISPR/Cas12b assay, indicating complete positive agreement in this all-positive sample set. Because no negative samples were included, negative agreement, diagnostic specificity, false-positive rate, and overall clinical diagnostic accuracy could not be assessed. These results support further development of the assay as a potential screening tool; larger studies using well-characterized positive and negative clinical or field specimens are required.</p>","PeriodicalId":17663,"journal":{"name":"Journal of virological methods","volume":" ","pages":"115483"},"PeriodicalIF":1.7,"publicationDate":"2026-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149216375","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Corrigendum to "Development of a hamster kidney cell line expressing stably T7 RNA polymerase using retroviral gene transfer technology for efficient rescue of infectious foot-and-mouth disease virus" [J. Virol. Methods 156 (2009) 129-137]. “利用逆转录病毒基因转移技术开发稳定表达T7 RNA聚合酶的仓鼠肾细胞系有效拯救传染性口蹄疫病毒”的勘误[J]。性研究。方法[156(2009)129-137]。

IF 1.7 4区医学

Journal of virological methods Pub Date : 2026-10-02 DOI: 10.1016/j.jviromet.2026.115474

Haixue Zheng, Hong Tian, Ye Jin, Jinyan Wu, Youjun Shang, Shuanghui Yin, Xiangtao Liu, QingGe Xie

引用次数: 0

Exploring the potential therapeutic effects of ultradiluted Crotalus horridus on antibody-dependent enhancement in dengue virus pathogenesis 探讨超稀释牛角蒿对登革热病毒发病机制中抗体依赖性增强的潜在治疗作用

IF 1.6 4区医学

Journal of virological methods Pub Date : 2026-06-01 Epub Date: 2026-02-11 DOI: 10.1016/j.jviromet.2026.115365

Tanusree Ghorai , Avipsha Sarkar , Anirban Roy , Debadatta Nayak , Satadal Das

{"title":"Exploring the potential therapeutic effects of ultradiluted Crotalus horridus on antibody-dependent enhancement in dengue virus pathogenesis","authors":"Tanusree Ghorai , Avipsha Sarkar , Anirban Roy , Debadatta Nayak , Satadal Das","doi":"10.1016/j.jviromet.2026.115365","DOIUrl":"10.1016/j.jviromet.2026.115365","url":null,"abstract":"<div><div>DENV remains a global health concern, with ADE playing a major role in severe disease progression. ADE leads to excessive cytokine release, nitric oxide overproduction, and elevated levels of non-structural-1 antigen, contributing to higher vascular dysfunction, thrombocytopenia, and hemorrhagic manifestations. The present study investigated the therapeutic effect of ultradiluted <em>Crotalus horridus</em> on ADE-mediated DENV infection using <em>in vitro</em> system. MTT, colony formation, cytopathic effect observation, ELISA, and qRT-PCR, were performed to assess cytotoxicity, viral pathogenesis, and immunomodulatory effects. Ultradiluted <em>Crotalus horridus</em> showed no cytotoxicity at lower concentrations and preserved cell viability. Therapeutic treatment altered the expression of pro-inflammatory and anti-inflammatory mediators in HepG2 cells infected with DENV under ADE conditions. Furthermore, nitric oxide levels and non-structural 1 antigen secretion were significantly decreased, indicating a reduction in viral replication and associated pathogenic immunomodulation. Our study also revealed that the expression of TLRs were significantly downregulated during ADE of DENV infection. Following treatment with ultradiluted <em>Crotalus horridus</em>, TLR expression was enhanced, which might have aggravated the immune response during ADE in DENV infection. In conclusion, these findings demonstrate that ultradiluted <em>Crotalus horridus</em> may act as a potential therapeutic drug to alleviate the severity associated with ADE-mediated infection.</div></div>","PeriodicalId":17663,"journal":{"name":"Journal of virological methods","volume":"343 ","pages":"Article 115365"},"PeriodicalIF":1.6,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146172831","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Distribution of Lily symptomless virus in tissues of Lanzhou lily (Lilium davidii var. unicolor) by fluorescence in situ hybridisation 利用荧光原位杂交技术研究兰州百合组织中百合无症状病毒的分布。

IF 1.6 4区医学

Journal of virological methods Pub Date : 2026-06-01 Epub Date: 2026-02-15 DOI: 10.1016/j.jviromet.2026.115367

Panpan Yang , Xuefei Xu , Jingfang Wei , Wenliang Zhang , Guangya Bian , Leifeng Xu , Guodong Zhou , Jun Ming

{"title":"Distribution of Lily symptomless virus in tissues of Lanzhou lily (Lilium davidii var. unicolor) by fluorescence in situ hybridisation","authors":"Panpan Yang , Xuefei Xu , Jingfang Wei , Wenliang Zhang , Guangya Bian , Leifeng Xu , Guodong Zhou , Jun Ming","doi":"10.1016/j.jviromet.2026.115367","DOIUrl":"10.1016/j.jviromet.2026.115367","url":null,"abstract":"<div><div><em>Lily symptomless virus</em> (LSV) is one of the main viruses that endanger <em>Lilium davidii</em> var. <em>unicolor</em>, causing billions of losses to the lily planting industry every year. The distribution of viruses in plants is uneven and identifying the virus-free tissue regions in lily is crucial for obtaining virus-free resources through in vitro culture. In this study, <em>Lilium davidii</em> var. <em>unicolor</em> carrying LSV was used as the experimental material. RNA fluorescent probes were designed and prepared based on the genomic sequence of LSV. RNA in situ hybridization experiments were conducted on the meristematic tissues (root tips and stem tips), mature tissues (leaves, stem segments, pedicel), and flower organs (ovaries) to observe the distribution of LSV in different tissues. It was found that no fluorescence signal was detected in the apical meristematic tissue (0.65–0.8 mm × 0.2–0.25 mm) and pith cells of stem. Strong LSV signal expression was observed in the root tips, epidermis, cortex and vascular bundles of stem and pedicel, epidermis, palisade tissue, spongy tissue and vein vascular bundles of leaves, ovary and ovule. Due to the difficulty in the regeneration and differentiation of pith cells in stem segments, the apical meristem of <em>Lilium davidii</em> var. <em>unicolor</em> can be used as the starting material for obtaining virus-free resources through in vitro culture.</div></div>","PeriodicalId":17663,"journal":{"name":"Journal of virological methods","volume":"343 ","pages":"Article 115367"},"PeriodicalIF":1.6,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146213783","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cytokine-based rapid process control of live virus production: MCP-1 as a cross-family biomarker in Vero cells 基于细胞因子的活病毒生产快速过程控制：MCP-1在Vero细胞中作为跨家族生物标志物

IF 1.6 4区医学

Journal of virological methods Pub Date : 2026-06-01 Epub Date: 2026-02-24 DOI: 10.1016/j.jviromet.2026.115380

Johanna Bacher , Jana Barbero Calzado , Mario Nebenführ , Robert Schlegl , Alois Jungbauer

{"title":"Cytokine-based rapid process control of live virus production: MCP-1 as a cross-family biomarker in Vero cells","authors":"Johanna Bacher , Jana Barbero Calzado , Mario Nebenführ , Robert Schlegl , Alois Jungbauer","doi":"10.1016/j.jviromet.2026.115380","DOIUrl":"10.1016/j.jviromet.2026.115380","url":null,"abstract":"<div><div>Rapid determination of infectious virus titers is essential for efficient vaccine process development, yet conventional assays such as TCID50 and plaque assays require several days and limit throughput. Cytokine secretion represents a potential surrogate marker, enabling rapid detection of productive infection. Here, we evaluated cytokine secretion in Vero cells infected with live vaccine candidates from the <em>Flaviviridae</em> (Japanese encephalitis virus [JEV], yellow fever virus [YFV], Zika virus [ZIKV]) and <em>Togaviridae</em> (Chikungunya virus [CHIKV]) families. Cytokine profiling revealed consistent induction of MCP-1 across flavivirus infections, with RANTES detectable at lower intensity, while CHIKV infection suppressed cytokine release. MCP-1 secretion showed a strong quantitative correlation with infectious titers for JEV (R² = 0.86) and YFV (R² = 0.88), best described by logarithmic models at 18 and 24 h post infection. Additionally, MCP-1 dynamics reflected infection conditions, distinguishing medium and MOI effects in roller bottle production. These results establish MCP-1 as a rapid, cross-family biomarker of infectious virus replication and highlight its potential as a Process Analytical Technology (PAT) tool for automatable in-process monitoring and accelerated vaccine manufacturing.</div></div>","PeriodicalId":17663,"journal":{"name":"Journal of virological methods","volume":"343 ","pages":"Article 115380"},"PeriodicalIF":1.6,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147307297","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparison of whole-viral genome sequencing and PCR-based assay for anal HPV detection to inform clinical implementation 全病毒基因组测序与pcr检测肛门HPV的比较，为临床实施提供依据。

IF 1.6 4区医学

Journal of virological methods Pub Date : 2026-06-01 Epub Date: 2026-02-13 DOI: 10.1016/j.jviromet.2026.115366

Omar Harfouch , Derek MacMath , Poorani Subramanian , Rahwa Eyasu , Habib O. Ramadhani , Peiying Ye , Ashley Davis , Meredith Zoltick , Emade Ebah , Amay Shah , Amelia Cover , Phyllis Bijole , Rachel Silk , David Sternberg , Tina Liu , Grace Garrett , Miriam Jones , Randy Kier , Henry Masur , Shyamasundaran Kottilil , Andrea Lisco

{"title":"Comparison of whole-viral genome sequencing and PCR-based assay for anal HPV detection to inform clinical implementation","authors":"Omar Harfouch , Derek MacMath , Poorani Subramanian , Rahwa Eyasu , Habib O. Ramadhani , Peiying Ye , Ashley Davis , Meredith Zoltick , Emade Ebah , Amay Shah , Amelia Cover , Phyllis Bijole , Rachel Silk , David Sternberg , Tina Liu , Grace Garrett , Miriam Jones , Randy Kier , Henry Masur , Shyamasundaran Kottilil , Andrea Lisco","doi":"10.1016/j.jviromet.2026.115366","DOIUrl":"10.1016/j.jviromet.2026.115366","url":null,"abstract":"<div><div>To optimize anal cancer screening strategies, we compared two molecular platforms for anal high-risk Human Papillomavirus (HR-HPV) detection: whole-viral genome sequencing (WGS) and Cobas HPV-test-PCR (RC). Anal swabs from participants at high-risk for HPV acquisition were evaluated for cytological abnormalities and HR-HPV prevalence. Compared to WGS, RC had 51 % sensitivity and 98 % specificity for HPV16, 29 % and 92 % for HPV18, and 74 % and 93 % for non-16/18 HR-HPV. In a population with high prevalence of anal HR-HPV, RC had lower sensitivity in the detection of anal HR-HPV but was more robustly associated with abnormal anal cytology compared to WGS. In addition, WGS-based identification of HPV16 sub-lineages demonstrated that the composite A1, A2, A4, and C1 sub-lineages were associated with a higher risk of abnormal cytology compared to other HPV16 sub-lineages. WGS can further delineate the natural history of anal HR-HPV and their sub-lineages in populations at high-risk for HPV acquisition.</div></div>","PeriodicalId":17663,"journal":{"name":"Journal of virological methods","volume":"343 ","pages":"Article 115366"},"PeriodicalIF":1.6,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146201863","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Detection of cell impurities and NF-κB activation using SEAP reporter cell lines in vaccines 用SEAP报告细胞系检测疫苗细胞杂质和NF-κB活化。

IF 1.6 4区医学

Journal of virological methods Pub Date : 2026-06-01 Epub Date: 2026-02-18 DOI: 10.1016/j.jviromet.2026.115375

Sarah K. Mercier , Georgia Gilmayer , Jackson Williams , Jodie Latta , Pearl Bamford , Laura McMahon , Vidyani Manatunga , Anita Carscadden , Lisa Kerr

{"title":"Detection of cell impurities and NF-κB activation using SEAP reporter cell lines in vaccines","authors":"Sarah K. Mercier , Georgia Gilmayer , Jackson Williams , Jodie Latta , Pearl Bamford , Laura McMahon , Vidyani Manatunga , Anita Carscadden , Lisa Kerr","doi":"10.1016/j.jviromet.2026.115375","DOIUrl":"10.1016/j.jviromet.2026.115375","url":null,"abstract":"<div><h3>Introduction</h3><div>During the 2010 influenza season, the Therapeutic Goods Administration (TGA) reviewed adverse event (AE) signals of febrile convulsions in children following the administration of the seasonal Fluvax® trivalent vaccine. These AEs were attributed to nuclear factor kappa-beta (NF-κB) activation. In response, the TGA developed a method to detect NF-κB activation by vaccine samples.</div></div><div><h3>Methods</h3><div>Complementary Toll-Like Receptor (TLR)-expressing reporter cell lines THP1-Blue and Ramos-Blue enabled detection of TLR1–9 activation using the QUANTI-Blue™ system following incubation with a panel of TLR agonists, tumour necrosis factor-alpha (TNF-α), or vaccine sample.</div></div><div><h3>Results</h3><div>Using TNF-α as a positive control and specific Toll-like receptor (TLR) agonists, specificity was confirmed for each cell line based on extracellular (THP1-Blue) or endosomal (Ramos-Blue) TLR expression. Agonists for each cell line demonstrated linearity within the working range, met precision criteria for repeatability and precision. The assay tested viral protein, messenger RNA (mRNA), and bacterial protein vaccines, met accuracy parameter of 80–120 % recovery of spiked controls.</div></div><div><h3>Discussion</h3><div>We established and validated a robust, sensitive and reliable analytical approach for detecting possible NF-κB activation by bioactive components in vaccines.</div></div><div><h3>Interpretation</h3><div>This assay enables manufacturers and regulators to meet the growing demand for reliable impurity screening assays that support the characterization of vaccines activating the NF-κB pathway. Future studies must further characterise NF-κB activation by individual TLRs across a broader range of vaccines.</div></div>","PeriodicalId":17663,"journal":{"name":"Journal of virological methods","volume":"343 ","pages":"Article 115375"},"PeriodicalIF":1.6,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146258588","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development and application of truncated cap protein-based indirect ELISA for screening antibodies against porcine circovirus 3 基于截断帽蛋白间接ELISA法筛选猪圆环病毒3型抗体的建立与应用

IF 1.6 4区医学

Journal of virological methods Pub Date : 2026-06-01 Epub Date: 2026-02-18 DOI: 10.1016/j.jviromet.2026.115376

D. Dinesh , Sonalika Mahajan , P. Madhankumar , Monalisa Sahoo , Karikalan Mathesh , Gaurav Kumar Sharma , Amit Kumar , Triveni Dutt

{"title":"Development and application of truncated cap protein-based indirect ELISA for screening antibodies against porcine circovirus 3","authors":"D. Dinesh , Sonalika Mahajan , P. Madhankumar , Monalisa Sahoo , Karikalan Mathesh , Gaurav Kumar Sharma , Amit Kumar , Triveni Dutt","doi":"10.1016/j.jviromet.2026.115376","DOIUrl":"10.1016/j.jviromet.2026.115376","url":null,"abstract":"<div><div>Porcine circovirus type 3 (PCV3) is an emerging pathogen linked with reproductive failure, respiratory disease, dermatitis, nephropathy, and multisystemic inflammation, posing growing threats to the swine industry worldwide. Although molecular evidence of PCV3 circulation has been reported in India, there is no published information on its seroprevalence, leaving the extent of exposure and population immunity largely unknown. Serological tools are critical for epidemiological surveillance and monitoring vaccine responses; however, validated assays for PCV3 remain scarce. In the present study, we developed and evaluated an indirect enzyme-linked immunosorbent assay (ELISA) for the detection of PCV3-specific antibodies, based on a recombinant truncated Cap protein expressed in <em>Escherichia coli</em>. The antigenic fragment of the Cap gene, excluding the N-terminal nuclear localization signal, was used as coating antigen and assay parameters like optimal antigen concentration, serum dilution, and conjugate conditions, were standardized. The developed ELISA exhibited high specificity, with no cross-reactivity to antisera against other common porcine viruses, and the relative diagnostic sensitivity and specificity were estimated to be 97.0 % and 94.5 %, respectively. The assay also demonstrated strong repeatability and reproducibility and was validated at four different laboratories with κ- values indicating perfect agreement Application of the assay to field sera revealed widespread seropositivity (64.25 %), underscoring its utility for epidemiological surveillance and sero-monitoring of PCV3. This study provides the first indigenous ELISA with potential application in India, and is the first report indicating widespread seropositivity of PCV3 in India.</div></div>","PeriodicalId":17663,"journal":{"name":"Journal of virological methods","volume":"343 ","pages":"Article 115376"},"PeriodicalIF":1.6,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146258586","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Presentation of multiple copies of a non-dominant surface epitope by circular RNA effectively induce an immune response against SARS-CoV-2 环状RNA呈递非显性表面抗原表位的多个拷贝，可有效诱导针对SARS-CoV-2的免疫应答。

IF 1.6 4区医学

Journal of virological methods Pub Date : 2026-06-01 Epub Date: 2026-02-20 DOI: 10.1016/j.jviromet.2026.115378

Mehrasa Asghari , Masoud Golalipour , Ali Memarian , Touraj Farazmandfar

{"title":"Presentation of multiple copies of a non-dominant surface epitope by circular RNA effectively induce an immune response against SARS-CoV-2","authors":"Mehrasa Asghari , Masoud Golalipour , Ali Memarian , Touraj Farazmandfar","doi":"10.1016/j.jviromet.2026.115378","DOIUrl":"10.1016/j.jviromet.2026.115378","url":null,"abstract":"<div><h3>Background</h3><div>There is a pressing need for vaccine development platforms that are rapid, effective, and adaptable to emerging pathogens such as SARS-CoV-2. Circular RNA (circRNA) offers advantages in stability and non-immunogenicity over traditional mRNA approaches. We aimed to investigate the effect of increasing the amplification of a non-dominant surface epitope in the circRNA structure on the induction of an immune response to that epitope in a mouse model.</div></div><div><h3>Methods</h3><div>Two circRNA constructs containing one and ten copies of an epitope were designed using immunoinformatic methods, and their impact on immune responses was investigated. Immunogenicity in mice was assessed by antibody assays, virus neutralization assays, and cytokine analysis.</div></div><div><h3>Results</h3><div>The titer of anti-SARS-CoV-2 RBD-specific IgG antibody in mice treated with circRNA containing ten copies of the epitope was significantly higher than that in mice treated with circRNA containing a single copy of the epitope. The level of neutralizing antibodies in the circRNA containing ten copies of the epitope was also significantly higher than that in the single-copy group. In addition, the levels of cytokines IFN-γ, TNF-α, and IL-2 were significantly higher in the circRNA containing ten copies of the epitope than in the single copy group.</div></div><div><h3>Conclusion</h3><div>Combining copy number amplification of a candidate epitope with a circRNA platform could lead to a powerful construct for enhancing immune responses. This approach may offer significant potential for controlling emerging infectious diseases in the future.</div></div>","PeriodicalId":17663,"journal":{"name":"Journal of virological methods","volume":"343 ","pages":"Article 115378"},"PeriodicalIF":1.6,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147271582","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A lyophilized stabilizer formulation enhances thermostability and preserves the immunogenicity of Senecavirus A post thermal challenge 一种冻干稳定剂配方提高了赛尼卡病毒A热激后的热稳定性和免疫原性。

IF 1.6 4区医学

Journal of virological methods Pub Date : 2026-06-01 Epub Date: 2026-02-24 DOI: 10.1016/j.jviromet.2026.115379

Jiankun Huang, Huiying Zhou, Zhenru Hu, Fengshi Xie, Xiaobo Wen, Xuhua Ran

{"title":"A lyophilized stabilizer formulation enhances thermostability and preserves the immunogenicity of Senecavirus A post thermal challenge","authors":"Jiankun Huang, Huiying Zhou, Zhenru Hu, Fengshi Xie, Xiaobo Wen, Xuhua Ran","doi":"10.1016/j.jviromet.2026.115379","DOIUrl":"10.1016/j.jviromet.2026.115379","url":null,"abstract":"<div><div>Senecavirus A (SVA) is a pathogenic virus that causes vesicular lesions, locomotor disorders, and neonatal mortality in pigs. Its increasing global prevalence poses a persistent threat to the swine industry. Vaccination remains the primary control strategy; however, SVA exhibits pronounced thermal instability, and exposure to unsuitable temperatures during storage or transport can impair vaccine immunogenicity. We developed a heat-stable lyoprotectant formulation for live SVA vaccines to overcome this limitation. The formulation, composed of 10% trehalose, 2% inulin, 2% dextran 40, 2% mannitol, 2% polyethylene glycol, 5% tryptone, 1% glycine, and 1% gelatin, demonstrated superior preservation during lyophilization and significantly greater thermal stability than conventional skim milk protectants. It exhibited no cytotoxicity at a 10% concentration and induced strong neutralizing antibody responses in mice, even after heat exposure. Collectively, this study establishes an effective lyoprotectant formulation that enhances the thermal stability and immunogenic integrity of live SVA, offering a practical advancement for improving vaccine quality and field efficacy.</div></div>","PeriodicalId":17663,"journal":{"name":"Journal of virological methods","volume":"343 ","pages":"Article 115379"},"PeriodicalIF":1.6,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147307321","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0