抗人乳头瘤病毒IgY的制备及评价。

IF 2.2 4区医学 Q3 BIOCHEMICAL RESEARCH METHODS

Journal of virological methods Pub Date : 2025-02-06 DOI:10.1016/j.jviromet.2025.115115

Weiguang Chen , Huanxin Xiao , Mingxia Lin , Jiqing Zhou , Qiancheng Xuan , Xiping Cui , Suqing Zhao

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引用次数: 0

摘要

人乳头瘤病毒（HPV）感染是一项重大的全球健康挑战，与宫颈癌等疾病的发生密切相关。不幸的是，仍然缺乏有效的治疗方法。鉴于抗体药物的优势，抗体靶向治疗可能成为治疗和预防HPV感染的手段之一。本研究探讨了用HPV九价疫苗免疫来港鸡的抗体靶向治疗的潜力。用苦味-硫酸铵法从鸡蛋中提取卵黄抗体（IgY），并通过SDS-PAGE分析确认。假病毒中和抗体实验测定中和效价，可达1:2000 （18.2μg/mL）。这种成功制备的抗HPV 6/11/16/18/31/33/45/52/58-L1蛋白显示了其作为治疗剂的潜力，特别是在hpv16感染后。这项工作为hpv特异性IgY的制备奠定了基础，并有助于推进宫颈癌的靶向治疗，促进hpv相关治疗方法的进一步研究。

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Preparation and evaluation of IgY against human papillomavirus

Human papillomavirus (HPV) infection is a major global health challenge and is closely related to the occurrence of diseases such as cervical cancer. Unfortunately, effective treatments are still lacking. In view of the advantages of antibody drugs, antibody-targeted therapy may become one of the means of treatment and prevention of HPV infection. This study explores the potential of antibody-targeted therapy using immunization with HPV nine-valent vaccine in Leghorn chickens. The resulting egg yolk antibodies (IgY) was extracted from eggs using the bitter-ammonium sulfate method and confirmed through SDS-PAGE analysis. The neutralizing titer was performed by pseudovirus-neutralizing antibody experiments, which could reach 1:2000 (18.2 μg/mL). This successful preparation of IgY against HPV 6/11/16/18/31/33/45/52/58-L1 protein showed its potential as a therapeutic agent, particularly post-HPV16 infection. This work lays the groundwork for HPV-specific IgY preparation and contributes to advancing targeted therapies for cervical cancer, prompting further research in HPV-related therapeutic approaches.

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来源期刊

Journal of virological methods 医学-病毒学

CiteScore

5.80

自引率

0.00%

发文量

209

审稿时长

41 days

期刊介绍： The Journal of Virological Methods focuses on original, high quality research papers that describe novel and comprehensively tested methods which enhance human, animal, plant, bacterial or environmental virology and prions research and discovery. The methods may include, but not limited to, the study of: Viral components and morphology- Virus isolation, propagation and development of viral vectors- Viral pathogenesis, oncogenesis, vaccines and antivirals- Virus replication, host-pathogen interactions and responses- Virus transmission, prevention, control and treatment- Viral metagenomics and virome- Virus ecology, adaption and evolution- Applied virology such as nanotechnology- Viral diagnosis with novelty and comprehensive evaluation. We seek articles, systematic reviews, meta-analyses and laboratory protocols that include comprehensive technical details with statistical confirmations that provide validations against current best practice, international standards or quality assurance programs and which advance knowledge in virology leading to improved medical, veterinary or agricultural practices and management.