Preparation and evaluation of IgY against human papillomavirus

IF 2.2 4区医学 Q3 BIOCHEMICAL RESEARCH METHODS

Journal of virological methods Pub Date : 2025-02-06 DOI:10.1016/j.jviromet.2025.115115

Weiguang Chen , Huanxin Xiao , Mingxia Lin , Jiqing Zhou , Qiancheng Xuan , Xiping Cui , Suqing Zhao

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引用次数: 0

Abstract

Human papillomavirus (HPV) infection is a major global health challenge and is closely related to the occurrence of diseases such as cervical cancer. Unfortunately, effective treatments are still lacking. In view of the advantages of antibody drugs, antibody-targeted therapy may become one of the means of treatment and prevention of HPV infection. This study explores the potential of antibody-targeted therapy using immunization with HPV nine-valent vaccine in Leghorn chickens. The resulting egg yolk antibodies (IgY) was extracted from eggs using the bitter-ammonium sulfate method and confirmed through SDS-PAGE analysis. The neutralizing titer was performed by pseudovirus-neutralizing antibody experiments, which could reach 1:2000 (18.2 μg/mL). This successful preparation of IgY against HPV 6/11/16/18/31/33/45/52/58-L1 protein showed its potential as a therapeutic agent, particularly post-HPV16 infection. This work lays the groundwork for HPV-specific IgY preparation and contributes to advancing targeted therapies for cervical cancer, prompting further research in HPV-related therapeutic approaches.

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来源期刊

Journal of virological methods 医学-病毒学

CiteScore

5.80

自引率

0.00%

发文量

209

审稿时长

41 days

期刊介绍： The Journal of Virological Methods focuses on original, high quality research papers that describe novel and comprehensively tested methods which enhance human, animal, plant, bacterial or environmental virology and prions research and discovery. The methods may include, but not limited to, the study of: Viral components and morphology- Virus isolation, propagation and development of viral vectors- Viral pathogenesis, oncogenesis, vaccines and antivirals- Virus replication, host-pathogen interactions and responses- Virus transmission, prevention, control and treatment- Viral metagenomics and virome- Virus ecology, adaption and evolution- Applied virology such as nanotechnology- Viral diagnosis with novelty and comprehensive evaluation. We seek articles, systematic reviews, meta-analyses and laboratory protocols that include comprehensive technical details with statistical confirmations that provide validations against current best practice, international standards or quality assurance programs and which advance knowledge in virology leading to improved medical, veterinary or agricultural practices and management.