Journal of Vector Borne Diseases最新文献

{"title":"Malaria and dengue outbreaks: a double health threat in southeastern Iran.","authors":"Jalil Nejati, Rubén Bueno-Marí","doi":"10.4103/JVBD.JVBD_186_23","DOIUrl":"https://doi.org/10.4103/JVBD.JVBD_186_23","url":null,"abstract":"","PeriodicalId":17660,"journal":{"name":"Journal of Vector Borne Diseases","volume":null,"pages":null},"PeriodicalIF":0.5,"publicationDate":"2024-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140864439","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Computational design, docking, and molecular dynamics simulation study of RNA helicase inhibitors of dengue virus.","authors":"Raghunath Satpathy, Sonali Acharya, Rashmiranjan Behera","doi":"10.4103/JVBD.JVBD_188_23","DOIUrl":"https://doi.org/10.4103/JVBD.JVBD_188_23","url":null,"abstract":"<p><strong>Background and objectives: </strong>RNA viruses are complex pathogens in terms of their genetic makeup, mutation frequency, and transmission modes. They contain the RNA helicase enzyme, which plays a crucial role in the viral genome replication process. This work aims to develop and screen a potential molecule that could function as a dengue virus (DENV) RNA helicase inhibitor.</p><p><strong>Methods: </strong>The present study was performed by taking 26 potential derivatives of gedunin phytochemicals from the PubChem database as ligands. The binding study of the compounds were analyzed by in silico docking method considering DENV RNA helicase enzyme as the receptor.</p><p><strong>Results: </strong>After a thorough analysis of the docking scores, toxicity, and physicochemical properties, compound tetrahydrogedunin was obtained as the best. Based on tetrahydrogedunin molecular structure, 100 drug-like molecules were designed using the Data Warrior tool. After the screening process for drug-likeness and ADMET properties, the derivative number 42 was considered as the promising. Further comparative docking of derivative 42 and a standard inhibitor molecule ST-610 with DENV RNA helicase enzyme showed binding affinity as -10.0 kcal/mol and -9.6 kcal/mol, respectively. The favorable interaction between DENV RNA helicase and derivative 42 was further validated by 50 nanoseconds molecular dynamics simulation and MM-GBSA analysis.</p><p><strong>Interpretation conclusion: </strong>Since the antiviral activity of derivative 42 has not been reported till date, the compound was predicted as a novel therapeutic molecule that can act against the dengue virus (DENV) RNA helicase enzyme.</p>","PeriodicalId":17660,"journal":{"name":"Journal of Vector Borne Diseases","volume":null,"pages":null},"PeriodicalIF":0.5,"publicationDate":"2024-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140849091","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of radiation-induced sterility, longevity, and reproductive characteristics of the Aedes aegypti (Diptera: Culicidae) strain of Swat: A step towards the implementation of SIT in Pakistan.","authors":"Inamullah Khan, Gul Zamin Khan, Jehangir Khan, Muhammad Amin","doi":"10.4103/JVBD.JVBD_131_23","DOIUrl":"https://doi.org/10.4103/JVBD.JVBD_131_23","url":null,"abstract":"<p><strong>Background objectives: </strong>Biological approaches for vector mosquito control such as sterile insect technique (SIT) requires sterilization of male mosquitoes through specific radiation doses for sterility induction in males. Under the SIT program, the males used must be compatible with the wild males in vigor, flight and selection of mate. Much of this potential is determined by the diet provided in the larval stages and optimizing the irradiation doses that cause complete sterility but have very minimal effect on the lives of irradiated males. The current study is designed to evaluate gamma radiation doses for inducing sterility in male Aedes aegypti mosquitoes with a genomic background from Pakistan (Swat district) and to assess the effects of radiation exposure on the competency of irradiated males and other life traits of irradiated mosquitoes.</p><p><strong>Methods: </strong>Sterilization of 17-hour-old male pupae (groups of 50 pupa/cup in three replicates) of the Ae. aegypti Swat strain was conducted using radiation doses of 30, 60, 70, 90, and 105 gray (Gy) from (Co60) at the Nuclear Institute for Food and Agriculture (NIFA) in Peshawar, Pakistan. Post-irradiated pupae were released in adult cages. Virgin females of the same cohort were released (1:1) to mate with the irradiated males. Mortality, longevity, mating competitiveness of males, and female fecundity were recorded.</p><p><strong>Results: </strong>An average of 71 eggs per female was recorded in control with 86% hatch rate. Individual females mated with a 60 Gy treated males produced 60 ± 0.6 eggs per female with 17% hatch rate, and those mated with 70 Gy males produced 42 ± 0.01 eggs with a nil hatch rate, whereas females mated with males treated with ≥70 Gy doses did not reproduce to next generation. Females in groups of 50 mated with 60, 70 Gy treated males (equal pairs), produced 369±1.3 and 98±0.01 eggs with 15% and zero hatch rate. Significant dose dependent reduction in longevity was observed for >30 Gy doses. The matting competence of irradiated males was about half that of un-irradiated males.</p><p><strong>Interpretation conclusion: </strong>Aedes aegypti with a Pakistani genomic background treated with a ≥70 Gy dose of gamma radiation induced complete sterility in males and provided the first- step foundation for SIT application in Pakistan. Further extensive studies are required to optimize the SIT techniques so that fully sterile males with very minor quality changes can be produced on large scales for field trials.</p>","PeriodicalId":17660,"journal":{"name":"Journal of Vector Borne Diseases","volume":null,"pages":null},"PeriodicalIF":0.5,"publicationDate":"2024-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140852236","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigating public understanding and actions regarding Aedes mosquitoes and dengue prevention across altitudinal range in central Nepal.","authors":"Punya Ram Sukupayo, Ram Chandra Poudel, Tirth Raj Ghimire","doi":"10.4103/JVBD.JVBD_24_24","DOIUrl":"https://doi.org/10.4103/JVBD.JVBD_24_24","url":null,"abstract":"<p><strong>Backgrounds objectives: </strong>Dengue fever, transmitted by Aedes mosquitoes, is a significant public health concern in Nepal. An extensive Knowledge, Attitude, and Practice (KAP) survey related to Aedes mosquitoes and the prevention of dengue was conducted along the route from Matihani (62 m asl) to Kalinchok (3840 m asl) in central Nepal. This research examined residents' awareness of Aedes mosquitoes and the diseases they transmit, aiming to support the World Health Organization's national and global fight against mosquito-borne diseases.</p><p><strong>Methods: </strong>The survey included 550 participants aged 18-75 years (249 males and 201 females). Door-to-door campaigns were conducted to gather data on knowledge scores, practices, and attitudes about Aedes mosquitoes using a standard structured questionnaire.</p><p><strong>Results: </strong>Findings of this study revealed a moderate level of knowledge across the study area, with a mean knowledge score of 5.525±2.58 (correct answer rate of 46.05%). While 99.6% of respondents were aware of dengue transmission by mosquitoes, only 64.5% were able to report Aedes mosquitoes as the key transmitter. Less than one-third of the participants (27.8%) were familiar with black-and-white stripes in Aedes. Additionally, only 36.7% were knowledgeable about the day-biting behavior of Aedes mosquitoes.</p><p><strong>Interpretation conclusion: </strong>This KAP survey provides valuable insights into community understanding of Aedes mosquitoes and dengue prevention practices associated with various Nepalese communities residing in low to high altitudes of Central Nepal. The findings of this study can be adopted to address the increasing health burden faced by the countries with low- to-high altitude regions within the Hindu Kush Himalaya.</p>","PeriodicalId":17660,"journal":{"name":"Journal of Vector Borne Diseases","volume":null,"pages":null},"PeriodicalIF":0.5,"publicationDate":"2024-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140851462","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dengue dynamics: Prognostic and disease monitoring through molecular and serological profiling of clinical isolates.","authors":"Vikas Tiwari, Mohd Afzal, Abhishek Sharma, Jaishree Tiwari","doi":"10.4103/JVBD.JVBD_202_23","DOIUrl":"https://doi.org/10.4103/JVBD.JVBD_202_23","url":null,"abstract":"BACKGROUND OBJECTIVES\u0000Dengue fever is a mosquito-borne illness that affects millions of people worldwide every year. With no vaccination available, early detection and treatment is critical. One-hundred-twelve countries in the world pose a risk to travelers, particularly in metropolitan areas. Laboratory diagnoses vary according to objectives, resources, and schedule, with sensitivity and specificity must be balanced for effective testing.\u0000\u0000\u0000METHODS\u0000The current work is a cross-sectional diagnostic study and samples from suspected patients of dengue was collected from May 15 to November 15 2023 and transported to laboratory, and RT-PCR and Dengue Duo Rapid test diagnosis techniques were used on 48 clinical samples included in this study.\u0000\u0000\u0000RESULTS\u0000Blood was collected from suspected cases of dengue and subjected further to different molecular and serological parameters. Serum was separated from all 48 blood samples. RNA was isolated by silica column extraction method which is further utilized as a template for amplification and detection of dengue serotyping. Master Mix was prepared for the amplification and detection of dengue virus by Rotor-Gene Q Real-Time PCR Machine and further serological profiling of positive dengue cases was studied by conventional PCR.\u0000\u0000\u0000INTERPRETATION CONCLUSION\u0000Our laboratory effectively standardized an RT-PCR-based approach for molecular identification of dengue virus in clinical specimens. This adaptive technique which used numerous primer sets displayed good specificity and sensitivity in serotype detection. The technology provides for quick and reliable identification of dengue virus infections, allowing for targeted treatment and preventative actions for successful disease management in highly populated regions.","PeriodicalId":17660,"journal":{"name":"Journal of Vector Borne Diseases","volume":null,"pages":null},"PeriodicalIF":0.5,"publicationDate":"2024-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140687639","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dengue encephalitis: A rare manifestation of dengue fever.","authors":"Hewa Babarandage Chathurika Harshani, D. Ruwan, G. Chathuranga, Dedunu C U Dias Weligamagea, Janaki Abeynayake","doi":"10.4103/JVBD.JVBD_189_23","DOIUrl":"https://doi.org/10.4103/JVBD.JVBD_189_23","url":null,"abstract":"Dengue fever, caused by the dengue virus transmitted by mosquitoes, usually manifests as flu-like symptoms and is a prevalent tropical illness. However, there are rare cases where the infection takes an unusual course, resulting in severe complications like dengue encephalitis. This case report delineates an occurrence of dengue encephalitis in a patient from Sri Lanka. This work provides insights into the clinical presentation, diagnostic difficulties, and treatment approaches linked to this uncommon manifestation of dengue fever.","PeriodicalId":17660,"journal":{"name":"Journal of Vector Borne Diseases","volume":null,"pages":null},"PeriodicalIF":0.5,"publicationDate":"2024-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140688697","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Social, behavioral and environmental determinants of vector-borne diseases: A narrative review of evidence and implications for integrated control approaches.","authors":"Ahmad Y Alqassim","doi":"10.4103/JVBD.JVBD_34_24","DOIUrl":"https://doi.org/10.4103/JVBD.JVBD_34_24","url":null,"abstract":"Vector-borne diseases exert immense health burdens worldwide. Malaria alone causes over 200 million cases and 600,000 deaths annually. Transmission involves complex drivers requiring examination beyond entomological factors. A systematic literature search across databases identified relevant studies on vector-borne disease determinants published in the last two decades, with priority given to rigorous designs like longitudinal analyses, reviews, and meta-analyses from diverse epidemic regions, allowing narrative synthesis of key determinants, relationships, and gaps. This narrative review synthesizes evidence on social, behavioral, and environmental determinants of major vector-borne disease risks over the past decade. Lower education, poverty, unplanned urbanization, gender inequities, inadequate water and waste management, climate variations, and land-use changes stood out as key determinants. However, significant knowledge gaps remain around quantifying precise threshold effects and impacts of tailored interventions across contexts. A social-ecological perspective recognizing interdependencies between determinants should frame integrated control programs. Multicomponent strategies addressing environmental modifications, protective measures, behavioral motivators, and infrastructure alongside governance and community engagement require implementation research and impact evaluation. Overall, this review highlights priority areas for advancing evidence-based vector control through contextualized, determinant-targeted policies and interventions. Further research incorporating modeling, trials, and cost-effectiveness analyses is critical to validate approaches that address influential drivers, leverage motivators, and reduce the global burden.","PeriodicalId":17660,"journal":{"name":"Journal of Vector Borne Diseases","volume":null,"pages":null},"PeriodicalIF":0.5,"publicationDate":"2024-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140686648","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Shifting spatial, temporal and demographic patterns of dengue incidence and associated meteorological factors in Jazan Region of Saudi Arabia from 2015-2020.","authors":"Ahmad Y Alqassim, M. Badedi, Mohammed A. Muaddi, Abdullah A. Alharbi, Mohammad A Jareebi, Anwar M Makeen, M. El-Setouhy, Osama B Albasheer, Abdullah Sabai, Ahmed Sahly","doi":"10.4103/JVBD.JVBD_15_24","DOIUrl":"https://doi.org/10.4103/JVBD.JVBD_15_24","url":null,"abstract":"BACKGROUND\u0000Dengue poses a considerable public health threat in Saudi Arabia, with escalating outbreaks in Jazan, where seasonal rains create ideal mosquito breeding conditions. Elucidating local epidemiological dynamics is imperative to strengthen evidence-based prevention policies. This study analyzed the spatiotemporal, demographic, and meteorological patterns of dengue in Jazan from 2015-2020.\u0000\u0000\u0000METHODS\u0000This retrospective cross-sectional study utilized surveillance records for 3427 confirmed dengue cases. Descriptive analyses characterized geographic, seasonal, age, sex, and nationality distributions. Forecasting models project expected epidemics through 2025. Regression analysis identified climate factors associated with monthly case counts.\u0000\u0000\u0000RESULTS\u0000Dengue exhibited shifting seasonal peaks, transitioning into year-round transmission by 2019, indicating endemic establishment. Cases clustered in different high-burden sectors annually, requiring localized vector control. The majority of affected individuals were young male adults, with gender gaps narrowing over time. Saudi nationals had an escalating incidence, but non-citizens showed a higher risk, signaling importation threats. Seasonal outbreaks were associated with temperature, wind speed, and direction.\u0000\u0000\u0000CONCLUSION\u0000Enhanced surveillance, outbreak forecasting, targeted control activities, and integrated prevention policies grounded in continuous evidence assessment can effectively address endemic dengue transmission in Jazan. This study provides key insights to optimize data-driven decision-making for dengue control in Saudi Arabia.","PeriodicalId":17660,"journal":{"name":"Journal of Vector Borne Diseases","volume":null,"pages":null},"PeriodicalIF":0.5,"publicationDate":"2024-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140687665","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A comparative study on vector competence of Anopheles stephensi from geographically distinct malarious and a non-malarious urban area to Plasmodium vivax.","authors":"J. Ravindran, Alex Eapen","doi":"10.4103/JVBD.JVBD_7_24","DOIUrl":"https://doi.org/10.4103/JVBD.JVBD_7_24","url":null,"abstract":"BACKGROUND OBJECTIVES\u0000Anopheles stephensi is responsible for the transmission of malaria in urban areas. Vector competence of An. stephensi from a non-malarious (Coimbatore) and highly malarious (Chennai) urban areas in Tamil Nadu state of India, was investigated to find the reason for non-transmission of malaria in Coimbatore.\u0000\u0000\u0000METHODS\u0000Vector competence (susceptibility/refractoriness) of An. stephensi mosquitoes from Chennai (malarious) and Coimbatore (non-malarious) to Plasmodium vivax (Chennai) was investigated. Bioassays were carried out concurrently in both these strains by artificial membrane feeding technique using the same malaria-infected blood. An. stephensi were dissected to observe infection in midgut and salivary gland. The parasite infection, oocyst and sporozoite positivity rate, the oocyst load, correlation between male-female gametocyte ratio and infection, and Survival Analysis of parasitic stages during sporogony were analyzed and compared.\u0000\u0000\u0000RESULTS\u0000The overall infection rate was 45.8 and 41.2 percent in Chennai and Coimbatore, respectively. Oocyst count ranged from 1-80 and 1-208 respectively and not statistically significant. Oocyst positivity was high from Day 8-21 in both strains. The Mean Survival Day (MSD) for oocyst was Day 14 in both strains. Sporozoite was observed in four experiments in each of the strains and the MSD for sporozoites was Day 20 in Chennai and Day 17 in Coimbatore.\u0000\u0000\u0000INTERPRETATION CONCLUSION\u0000An. stephensi of Chennai and Coimbatore are equally susceptible to P. vivax infection and non-transmission of malaria in Coimbatore can be attributed to external factors such as the presence of preferential breeding habitat, vector density, vector survival, and weather. The only difference observed was the comparatively shortened oocyst maturation time in the Coimbatore strain which requires further investigation.","PeriodicalId":17660,"journal":{"name":"Journal of Vector Borne Diseases","volume":null,"pages":null},"PeriodicalIF":0.5,"publicationDate":"2024-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140712053","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioacoustics in population control of insects of medical importance: A review.","authors":"Udita Mandal, J. Dutta","doi":"10.4103/JVBD.JVBD_204_23","DOIUrl":"https://doi.org/10.4103/JVBD.JVBD_204_23","url":null,"abstract":"Vector transmitted diseases are accountable for more than 17% of all infectious disease cases worldwide according to World Health Organization. Insect vectors play a key role in transmitting diseases and loss of lives. Modified and advanced vector control strategies with chemical insecticides are needed as vectors are resistant to a particular insecticide. Moreover, chemical control is cost-inductive and may give rise to health issues. In this review, bioacoustics have been narrated as a novel technology for eco-friendly and cost-effective control of insect vectors. Many insects that rely on sounds for communication and copulation can be trapped, killed, and repelled through the mimicked sounds of conspecific males or females, generating disturbing noises. Sound can also be harnessed to prevent the mating success of insects. There is need for future studies on rejection calls and harmonic convergences in insect vectors. In-depth investigations on the higher harmonics of insect calls, along with artificial intelligence, will be beneficial for the development of successful sound- evoked control of insects of medical importance.","PeriodicalId":17660,"journal":{"name":"Journal of Vector Borne Diseases","volume":null,"pages":null},"PeriodicalIF":0.5,"publicationDate":"2024-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140712093","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}