Mangala K A Sudarshani, T Eswaramohan, A Murugananthan, H C E Wegiriya, V N H de Silva, P L A N Liyanage, S N Surendran
{"title":"Distribution changes of cutaneous leishmaniasis: A postulated risk of disease transmission in Hambantota, Sri Lanka.","authors":"Mangala K A Sudarshani, T Eswaramohan, A Murugananthan, H C E Wegiriya, V N H de Silva, P L A N Liyanage, S N Surendran","doi":"10.4103/jvbd.jvbd_117_23","DOIUrl":"10.4103/jvbd.jvbd_117_23","url":null,"abstract":"<p><strong>Background objectives: </strong>Leishmaniasis is caused by various species of parasite Leishmania. Approximately twenty of them are pathogenic to mammals. In Sri Lanka, cutaneous leishmaniasis (CL) is an established vector-borne disease. CL originates and spreads mainly through sandfly bite in many endemic countries. The aim of the present study was to compare the geographical distribution and demographic features of CL cases in Hambantota district, Sri Lanka in 2014 and 2016.</p><p><strong>Methods: </strong>The patients who were presented to the Tangalle Base Hospital from June to December in 2014 and 2016 were examined and a descriptive study was carried out using a structured-questionnaire. Slit-skin smears were collected from each patient, Giemsa-stained and examined under the light microscope to identify Leishmania amastigotes.</p><p><strong>Results: </strong>Out of 256 and 314 suspected CL patients, 156 and 155 were identified positive for the year 2014 and 2016, respectively. Out of 12 District Secretary Divisions (DSD) in Hambantota district, the highest number of CL cases, 85 and 86 was reported from Tangalle DSD in 2014 and 2016 respectively. Number of identified CL patients in Beliatta DSD had increased from 50 to 67 during the study period. In both years, majority of CL patients were ≥50 years old with males more infected than females. Although CL association with occupations were insignificant, housewives were the highly (23%) infected occupants in this area.</p><p><strong>Interpretation conclusion: </strong>Based on the present findings, geographical distribution within DSDs in Hambantota district had changed. This emphasizes the importance of CL as a health problem in Hambantota district.</p>","PeriodicalId":17660,"journal":{"name":"Journal of Vector Borne Diseases","volume":"61 2","pages":"275-280"},"PeriodicalIF":0.8,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141450780","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Nisa Fauziah, Fedri Ruluwedrata Rinawan, Naufal Fakhri Nugraha, Lia Faridah, Karomahul Malaya Jati, Angelina Dakosta, Mahatyanta Kalya Santika, Muhammad Yusuf Zakiyyudin, Ahmad Muhsin, Kiky Furbani Rizkillah, Miftahul Nurun Nisa, Ryan Bayusantika Ristandi
{"title":"Malaria elimination in West Java, Indonesia: A descriptive-and-qualitative study.","authors":"Nisa Fauziah, Fedri Ruluwedrata Rinawan, Naufal Fakhri Nugraha, Lia Faridah, Karomahul Malaya Jati, Angelina Dakosta, Mahatyanta Kalya Santika, Muhammad Yusuf Zakiyyudin, Ahmad Muhsin, Kiky Furbani Rizkillah, Miftahul Nurun Nisa, Ryan Bayusantika Ristandi","doi":"10.4103/jvbd.jvbd_113_23","DOIUrl":"10.4103/jvbd.jvbd_113_23","url":null,"abstract":"<p><strong>Background objectives: </strong>Following World Health Organization (WHO) plans for thirty-five malaria-endemic countries, Indonesia will eliminate malaria by 2030. As one of the Indonesian provinces, West Java targeted subnational malaria elimination in 2022. This article aims to describe malaria surveillance data and elimination programs, including weaknesses in sustaining the program.</p><p><strong>Methods: </strong>This study used secondary data from malaria surveillance information system regencies/cities' case reports for 2019-2022 and achievement data of sub-national malaria elimination certification from each regency/city from 2014-2022. The data was confirmed from the evaluation study document, analysis of reported cases, and interviews.</p><p><strong>Results: </strong>Most cases were confirmed by microscopic examination (84.1% in 2021 and 94.4% in 2022) and rapid diagnostic tests (57% in 2019 and 58.1% in 2020). Malaria is more prevalent among men (93% in 2019, 95% in 2020, 96% in 2021, and 95.9% in 2022) and productive ages of 15-64 years (98.8% in 2019, 100% in 2020, 99.2% in 2021, and 98.8% in 2022), frequently occurs in the military (56.3% in 2019, 75.7% in 2020, 45.2% in 2021) and police (40.5% in 2022), often uses passive case detection for identifying cases (97.9% in 2019 and 2020, 95.2% in 2021, and 97.6% in 2022), and the majority undergo inpatient treatment (86.4% in 2019, 81.7% in 2021, and 82.6% in 2022). Most positive cases originated from imported cases, and last indigenous cases were still found in 2019. Plasmodium vivax dominated malaria cases and and relapses were high (55.0% in 2020, and 47.3% in 2022).</p><p><strong>Interpretation conclusion: </strong>All regencies/cities have obtained sub-national malaria elimination certification in 2022. West Java has the potential to be verified for Java-Bali sub-national malaria elimination targeted in 2023, albeit cases of imported malaria still occur. It is imperative to address the issue of imported cases transitioning into locally transmitted cases (introduced) by effective coordination across all regencies/cities and inter-provincial efforts.</p>","PeriodicalId":17660,"journal":{"name":"Journal of Vector Borne Diseases","volume":"61 2","pages":"183-194"},"PeriodicalIF":0.8,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141450823","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"In silico screening of potential plant peptides against the non-structural proteins of dengue virus.","authors":"G Reena, R Ranjani, D Goutham, K Sangeetha","doi":"10.4103/jvbd.jvbd_47_23","DOIUrl":"10.4103/jvbd.jvbd_47_23","url":null,"abstract":"<p><strong>Background objectives: </strong>Peptides isolated from different sources of plants have the advantages of specificity, lower toxicity, and increased therapeutic effects; hence, it is necessary to search for newer antivirals from plant sources for the treatment of dengue viral infections.</p><p><strong>Methods: </strong>In silico screening of selected plant peptides against the non-structural protein 1, NS3 protease domain (NS2B-NS3Pro) with the cofactor and ATPase/helicase domain (NS3 helicase domain/NS3hel) of dengue virus was performed. The physicochemical characteristics of the peptides were calculated using Protparam tools, and the allergenicity and toxicity profiles were assessed using allergenFP and ToxinPred, respectively.</p><p><strong>Results: </strong>Among the tested compounds, Ginkbilobin demonstrated higher binding energy against three tested nonstructural protein targets. Kalata B8 demonstrated maximum binding energy against NSP-1 and NSP-2, whereas Circulin A acted against the NSP3 protein of dengue virus.</p><p><strong>Interpretation conclusion: </strong>The three compounds identified by in silico screening can be tested in vitro, which could act as potential leads as they are involved in hampering the replication of the dengue virus by interacting with the three prime non-structural proteins.</p>","PeriodicalId":17660,"journal":{"name":"Journal of Vector Borne Diseases","volume":"61 2","pages":"211-219"},"PeriodicalIF":0.8,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141450782","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
U M Kurmi, N Nanvyat, M P Lapang, M J Mafuyai, I Luka, O Akwashiki, G I Yina, E O Otakpa, R L Simse, G S Mwansat
{"title":"Vectors, knowledge, attitudes, and practices in relation to malaria transmission in Bauchi State, Nigeria.","authors":"U M Kurmi, N Nanvyat, M P Lapang, M J Mafuyai, I Luka, O Akwashiki, G I Yina, E O Otakpa, R L Simse, G S Mwansat","doi":"10.4103/jvbd.jvbd_66_23","DOIUrl":"10.4103/jvbd.jvbd_66_23","url":null,"abstract":"<p><strong>Background objectives: </strong>Malaria remains a major public health concern in Nigeria and the vector (Anopheles species) can only be controlled effectively by having good knowledge of its composition and potential for disease transmission. This work aimed at surveying indoor malaria vectors and, Knowledge, Attitudes, and Practices (KAP) in relation to disease transmission in Toro LGA, Bauchi State, Nigeria.</p><p><strong>Methods: </strong>Mosquitoes were collected by Pyrethrum Spray Catch (PSC) between October and December 2019 and were morphologically identified using standard keys. Blood samples were collected from individuals who slept in the rooms where PSC was conducted. Thick and thin blood smears were made for malaria parasite examination. Questionnaires were administered to 120 participants for the KAP studies.</p><p><strong>Results: </strong>Ninety-seven Anopheles mosquitoes were collected and identified as Anopheles gambiae 76 (78.35%), An. funestus 20 (20.62%) and An. coustani 1(1.03%). The overall malaria prevalence was 15.8%. KAP studies revealed that measures and treatment-seeking behaviours against malaria varied significantly (P<0.05) among the respondents. Sleeping under a net 55 (45.8%) and use of insecticides 24 (20.0%) were some of the preventive measures highlighted while the treatment-seeking behaviours included visit to a pharmacy 74 (61.7%) and use of local herbs 11 (9.2%).</p><p><strong>Interpretation conclusion: </strong>This work revealed that An. gambiae and An. funestus are predominant malaria vectors in the area. The KAP information demonstrated fair knowledge about the disease by the respondents. Therefore, public enlightenment about malaria prevention, control and treatment is recommended to address the few but highly negative impact knowledge gaps about malaria.</p>","PeriodicalId":17660,"journal":{"name":"Journal of Vector Borne Diseases","volume":"61 2","pages":"176-182"},"PeriodicalIF":0.8,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141450829","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Unveiling malaria's silent threat: A path to effective prevention in India.","authors":"Ume Aiman, Zainab Azad, Abdul Raffay Awais","doi":"10.4103/jvbd.jvbd_165_23","DOIUrl":"10.4103/jvbd.jvbd_165_23","url":null,"abstract":"","PeriodicalId":17660,"journal":{"name":"Journal of Vector Borne Diseases","volume":"61 2","pages":"311"},"PeriodicalIF":0.8,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141450828","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Wan Siti Maryam Wan Nazri, Lau Yee Ling, Cheong Fei Wen
{"title":"Detection of Plasmodium knowlesi in whole blood samples with sandwich enzyme-linked immunosorbent assay (ELISA) using rhoptry-associated protein 1 specific polyclonal antibodies.","authors":"Wan Siti Maryam Wan Nazri, Lau Yee Ling, Cheong Fei Wen","doi":"10.4103/jvbd.jvbd_55_23","DOIUrl":"10.4103/jvbd.jvbd_55_23","url":null,"abstract":"<p><strong>Background objectives: </strong>Plasmodium knowlesi, a simian malaria species, is now known to infect humans. Due to disadvantages in the current diagnosis methods, many efforts have been placed into developing new methods to diagnose the disease. This study assessed the ability of the PkRAP-1 sandwich enzyme-linked immunosorbent (ELISA) to detect P knowlesi antigens in whole blood specimens.</p><p><strong>Methods: </strong>Western blot assay was conducted to evaluate the ability of raised mouse and rabbit anti-PkRAP-1 polyclonal antibodies to bind to the native proteins in P. knowlesi lysate. The polyclonal antibodies were then used in sandwich ELISA to detect P. knowlesi. In the sandwich ELISA, mouse and rabbit polyclonal antibodies were used as the capture and detection antibodies, respectively. The limit of detection (LOD) of the assay was determined using P. knowlesi A1H1 culture and purified recombinant PkRAP-1.</p><p><strong>Results: </strong>Western blot results showed positive reactions towards the proteins in P. knowlesi lysate. The LOD of the assay from three technical replicates was 0.068% parasitaemia. The assay performance in detecting P. knowlesi was 83% sensitivity and 70% specificity with positive and negative predictive values of 74% and 80%, respectively. The anti-PkRAP-1 polyclonal antibodies did not cross-react with P. falciparum and healthy samples, but P. vivax by detecting all 12 samples.</p><p><strong>Interpretation conclusion: </strong>PkRAP-1 has the potential as a biomarker for the development of a new diagnostic tool for P. knowlesi detection. Further studies need to be conducted to establish the full potential of the usage of anti-PkRAP-1 antibodies for P. knowlesi detection.</p>","PeriodicalId":17660,"journal":{"name":"Journal of Vector Borne Diseases","volume":"61 2","pages":"203-210"},"PeriodicalIF":0.8,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141450779","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Sandra Enríquez, Jazzmín Arrivillaga-Henríquez, Paul Duque, Vanessa Herrera, Franklin Vaca, Marco Sánchez, Mateo Roldán, Lenin Ron-Garrido, C Alfonso Molina
{"title":"Indicators sandflies and environment associated to spatial landscaping change in Chocó Biosphere Reserve UNESCO.","authors":"Sandra Enríquez, Jazzmín Arrivillaga-Henríquez, Paul Duque, Vanessa Herrera, Franklin Vaca, Marco Sánchez, Mateo Roldán, Lenin Ron-Garrido, C Alfonso Molina","doi":"10.4103/jvbd.jvbd_52_23","DOIUrl":"10.4103/jvbd.jvbd_52_23","url":null,"abstract":"<p><strong>Background objectives: </strong>Sandflies are vector insects associated with terrestrial forest ecosystems; in the Ecuadorian Andes, they participate in the transmission of human cutaneous leishmaniasis. This geographical area represents an opportunity to evaluate the role of sandflies as bioindicators of the degree of intervention of tropical humid forest ecosystems (THF) associated with changes in the ecology of the local landscape.</p><p><strong>Methods: </strong>CDC-light traps were used for collecting adult sandflies in February 2020 in a humid tropical forest within the Chocó Biosphere Reserve. All species were identified using morphological keys. Analysis data about abundance, richness, species accumulation, diversity index, species composition communities, species sex proportion, spatial sandflies environmental, Renyi's Diversity Profile were performed to compare six spatial habitats in Mashpi locality, Ecuador.</p><p><strong>Results: </strong>Sandflies were collected (n-1435); the main species are represented by Trichophoromyia reburra, Nyssomyia trapidoi, Psathyromyia aclydifera, Psychodopygus panamensis and Lutzomyia hartmanni. Only Th. reburra is associated with not intervened forest, while the other three species are associated with intervened forest within Mashpi in the Choco Biosphere Reserve. The secondary forest has major sandflies' richness, while the primary forest exhibits major abundance.</p><p><strong>Interpretation conclusion: </strong>Th. reburra is a sandfly restricted to the Andean Forest and is a bioindicator of the high environmental health quality of the forest, while Ny. trapidoi and Pa. aclydifera are bioindicators of environmental disturbances in the forest. Additionally, Ps. panamensis, Lu. hartmanni and Ny. trapidoi are bioindicators of human impact and the risk of leishmaniasis.</p>","PeriodicalId":17660,"journal":{"name":"Journal of Vector Borne Diseases","volume":"61 2","pages":"236-242"},"PeriodicalIF":0.8,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141450783","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The effectiveness of training mothers with regard to pediculosis preventive behavior among school students: A Protection Motivation Theory study.","authors":"Roghayeh Mohammadpour, Siamak Mohebi, Abolfazl Mohammadbeigi, Zeynab Gholamrezaee-Sarvelat, Roghaye Ahangari, Alireza Omidi Oskouei","doi":"10.4103/jvbd.jvbd_146_22","DOIUrl":"10.4103/jvbd.jvbd_146_22","url":null,"abstract":"<p><strong>Background objectives: </strong>Lice infestation is considered as a reemerging problem as well as a health challenge. Based on Protection Motivation Theory, the present study was conducted to investigate the effectiveness of training mothers in regard to pediculosis preventive behavior among school students.</p><p><strong>Methods: </strong>In a critical trial, 180 mothers of primary school daughters were assessed in two study groups in the Iranian city of Qom in 2021. They received training intervention in a course of four ninety-minute sessions during four weeks. After six months, the post-test questionnaire and the checklist were completed by them. The data were gathered using a valid and reliable questionnaire. Finally, data analysis was performed using SPSS 20.</p><p><strong>Results: </strong>After the training intervention, a significant difference was observed within the intervention group in scores of the prevention motivation constructs (P<0.001). Besides, there was a significant difference between the mean scores of the preventive behavior of the mothers and daughters in the intervention group before the training intervention and six months after it (P<0.001). However, no significant difference was seen before and after the intervention in the control group (P>0.05). Additionally, no significant differences were observed between the intervention and control groups (P<0.001) in terms of comparing the mean score differences of all the constructs of protection motivation theory, the mothers' behavior and the daughters' behavior checklist.</p><p><strong>Interpretation conclusion: </strong>The results indicate the effectiveness of training mothers based on Protection Motivation Theory in regard to pediculosis. Therefore, it seems that in developing training programs, utilizing this theory could prove useful to bring about changes in the individuals' behavior regarding the prevention of pediculosis.</p>","PeriodicalId":17660,"journal":{"name":"Journal of Vector Borne Diseases","volume":"61 2","pages":"195-202"},"PeriodicalIF":0.8,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141450826","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Structure-based virtual screening study for identification of potent insecticides against Anopheles gambiae to combat the malaria.","authors":"Nawal Helmi","doi":"10.4103/jvbd.jvbd_158_23","DOIUrl":"10.4103/jvbd.jvbd_158_23","url":null,"abstract":"<p><strong>Background objectives: </strong>Vector-borne infectious diseases contribute significantly to global mortality, with over 700,000 annual deaths, and malaria alone accounts for more than 400,000 of these fatalities. Anopheles gambiae, a prominent mosquito species, serves as a primary vector for transmitting malaria to humans. To address this issue, researchers have identified the D1-like dopamine receptor (DAR), specifically DOP2, as a promising target for developing new insecticides.</p><p><strong>Methods: </strong>The three-dimensional structure of DOP2 from A. gambiae was unavailable; in-silico approach was used to model and validate DOP2 structure. The Discovery Studio 2021 program was used to identify potential binding sites on DOP2. Virtual screening of 235 anti-parasitic compounds was performed against DOP2 using PyRx 0.8.</p><p><strong>Results: </strong>The screening demonstrated strong binding and interactions with active site residues of DOP2 for five compounds: Diclazuril, Kaempferol, Deracoxib, Clindamycin, and Diaveridine. These compounds exhibited higher binding affinity values compared to the control (Asenapine). In addition, the predicted physiochemical properties for these compounds were within acceptable ranges and there were no violations in drug-likeness properties.</p><p><strong>Interpretation conclusion: </strong>These compounds show promise as potential new insecticides targeting A. gambiae mosquito by inhibiting the DOP2 protein. However, additional experimental validation is required to optimize their efficacy as DOP2 inhibitors.</p>","PeriodicalId":17660,"journal":{"name":"Journal of Vector Borne Diseases","volume":"61 2","pages":"253-258"},"PeriodicalIF":0.8,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141450825","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Dhyanjyoti Sarma, Bhupamani Das, Niral Patel, Arun Patel, Abhinav Suthar, Ankit Prajapati, R M Patel
{"title":"Microscopic and molecular investigation of vector borne haemoprotozoan diseases in dromedary camel of North Gujarat, India.","authors":"Dhyanjyoti Sarma, Bhupamani Das, Niral Patel, Arun Patel, Abhinav Suthar, Ankit Prajapati, R M Patel","doi":"10.4103/jvbd.jvbd_105_23","DOIUrl":"10.4103/jvbd.jvbd_105_23","url":null,"abstract":"<p><strong>Background objectives: </strong>Vector-borne haemoprotozoan diseases comprise diverse group of single celled organism transmitted by haematophagus invertebrates. The current study was aimed at the identification of major haemoprotozoan (Babesia, Theileria and Trypanosoma) in dromedary camel of North Gujarat region in India using microscopy and Polymerase Chain Reaction (PCR).</p><p><strong>Methods: </strong>A total of 234 blood samples were screened by the microscopic and molecular detection assays. Molecular prevalence studies of Theileria, Trypanosoma spp and Babesia was undertaken using 18s ribosomal DNA, RoTat 1.2 and SS rRNA gene respectively. The data relating to microscopic and molecular prevalence along with associated risk factors were analysed by statistical methods.</p><p><strong>Results: </strong>The overall prevalence of hamoprotozoan disease based on microscopic and molecular investigation was 23.50%. The sensitivity and specificity (95% Confidence Interval) of PCR assay was 100% in comparison to microscopy (45.45 % sensitive and 100 % specific). The kappa coefficient between PCR and microscopy indicated good level of agreement with a value of 0.704 and SE of 0.159.</p><p><strong>Interpretation conclusion: </strong>Despite holding much significance to the animal sector, little work has been undertaken in regional parts of India regarding camel parasites. The present study offers first preliminary research data investigating haemoprotozoan disease using parasitological and molecular methods in camels in the region.</p>","PeriodicalId":17660,"journal":{"name":"Journal of Vector Borne Diseases","volume":"61 2","pages":"259-266"},"PeriodicalIF":0.8,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141450824","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}