Journal of Virus Eradication最新文献

{"title":"PP1.16 – 00059 Identification of solo-LTR HIV provirus in vitro","authors":"F. Li, G. Li, R. Gorelick, L. Kelly, F. Maldarelli","doi":"10.1016/j.jve.2024.100465","DOIUrl":"10.1016/j.jve.2024.100465","url":null,"abstract":"","PeriodicalId":17552,"journal":{"name":"Journal of Virus Eradication","volume":"10 ","pages":"Page 50"},"PeriodicalIF":3.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143181383","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of a latency model for HIV-1 subtype C and the impact of long terminal repeat element genetic variation on latency reversal","authors":"Shreyal Maikoo ,&nbsp;Robert-Jan Palstra ,&nbsp;Krista L. Dong ,&nbsp;Tokameh Mahmoudi ,&nbsp;Thumbi Ndung'u ,&nbsp;Paradise Madlala","doi":"10.1016/j.jve.2024.100575","DOIUrl":"10.1016/j.jve.2024.100575","url":null,"abstract":"<div><div>Sub-Saharan Africa accounts for almost 70 % of people living with HIV (PLWH) worldwide, with the greatest numbers centred in South Africa where 98 % of infections are caused by subtype C (HIV-1C). However, HIV-1 subtype B (HIV-1B), prevalent in Europe and North America, has been the focus of most cure research and testing despite making up only 12 % of HIV-1 infections globally. Development of latency models for non-subtype B viruses is a necessary step to address this disproportionate focus. Furthermore, the impact of genetic variation between viral subtypes, specifically within the long terminal repeat (LTR) element of the viral transcriptional promoter on latency reversal, remains unclear. To address this scientific gap, we constructed a minimal genome retroviral vector expressing HIV-1C consensus transactivator of transcription protein (Tat) and green fluorescent protein (GFP) under the control of either HIV-1C consensus LTR (C731CC) or the transmitted/founder (T/F) LTRs derived from PLWH (C_T/F731CC), produced corresponding LTR pseudotyped viruses using a vesicular stomatitis virus (VSV-G) pseudotyped Envelope vector and the pCMVΔR8.91 packaging vector containing HIV-1 accessory and <em>rev</em> genes. Viruses produced in this way were used to infect Jurkat E6 and primary CD4⁺ T cells <em>in vitro</em>. By enriching for latently infected cells, and treating them with different latency reversing agents, we developed an HIV-1C latency model that demonstrated that the HIV-1C consensus LTR has lower reactivation potential compared to its HIV-1B counterpart. Furthermore, HIV-1C T/F LTR pseudotyped proviral genetic variants exhibited a heterogenous reactivation response which was modulated by host cell (genetic) variation. Our data suggests that genetic variation both within and between HIV-1 subtypes influences latency reversal. Future studies should investigate the specific role of variation in host cellular environment on reactivation differences.</div></div>","PeriodicalId":17552,"journal":{"name":"Journal of Virus Eradication","volume":"10 4","pages":"Article 100575"},"PeriodicalIF":3.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11730875/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142983723","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PP3.1 – 00160 Macrophage Training Reactivates Latent HIV-1 from HAARTsuppressed PBMCs of PLWH","authors":"S. John,&nbsp;H. James,&nbsp;C. Perritano,&nbsp;I. Fraser","doi":"10.1016/j.jve.2024.100482","DOIUrl":"10.1016/j.jve.2024.100482","url":null,"abstract":"","PeriodicalId":17552,"journal":{"name":"Journal of Virus Eradication","volume":"10 ","pages":"Pages 59-61"},"PeriodicalIF":3.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143134581","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PP3.2 – 00156 Endogenous Cytokine Reporter Macrophages Facilitate Identification of Novel Training Compounds that can Reactivate and Initiate Clearance of Latent HIV","authors":"S.P. John,&nbsp;D. Dabral,&nbsp;C.M. Perritano,&nbsp;H. James,&nbsp;J. Marugan,&nbsp;M. Henderson,&nbsp;I.D.C. Fraser","doi":"10.1016/j.jve.2024.100483","DOIUrl":"10.1016/j.jve.2024.100483","url":null,"abstract":"","PeriodicalId":17552,"journal":{"name":"Journal of Virus Eradication","volume":"10 ","pages":"Page 61"},"PeriodicalIF":3.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143134582","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"6.3 – 00019 AAV Delivery of the CCR5-blocking monoclonal antibody Leronlimab yields long-term expression and ART-free remission from SHIV viremia","authors":"H.L. Wu,&nbsp;G.M. Webb,&nbsp;J. Zikos,&nbsp;D.M. Magnani,&nbsp;S.P. Fuchs,&nbsp;R.C. Desrosiers,&nbsp;J.B. Sacha","doi":"10.1016/j.jve.2024.100426","DOIUrl":"10.1016/j.jve.2024.100426","url":null,"abstract":"","PeriodicalId":17552,"journal":{"name":"Journal of Virus Eradication","volume":"10 ","pages":"Pages 21-22"},"PeriodicalIF":3.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143128837","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"6.4 – 00112 Overcoming immune responses directed toward AAV-delivered bNAbs","authors":"M. Kuipa,&nbsp;P. Koroma,&nbsp;I. Leguizamo,&nbsp;P. Dhole,&nbsp;M.R. Gardner","doi":"10.1016/j.jve.2024.100427","DOIUrl":"10.1016/j.jve.2024.100427","url":null,"abstract":"","PeriodicalId":17552,"journal":{"name":"Journal of Virus Eradication","volume":"10 ","pages":"Pages 22-23"},"PeriodicalIF":3.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143130657","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"5.3 – 00106 Macrophage-tropic TF SHIV D infected NHP model of reservoir persistence, decay and pathogenesis on suppressive anti-retroviral therapy","authors":"S. Mallick,&nbsp;R. Krause,&nbsp;A.G. Mcfarland,&nbsp;H. Schrader,&nbsp;G.D. Whitehill,&nbsp;F.E. Marino,&nbsp;R. Podgorski,&nbsp;E. Lewis,&nbsp;G.M. Shaw,&nbsp;T. Burdo,&nbsp;Katharine J. Bar","doi":"10.1016/j.jve.2024.100420","DOIUrl":"10.1016/j.jve.2024.100420","url":null,"abstract":"","PeriodicalId":17552,"journal":{"name":"Journal of Virus Eradication","volume":"10 ","pages":"Pages 17-18"},"PeriodicalIF":3.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143180806","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PP1.5 – 00132 Tunneling Nanotubes are essential for the propagation of HIV infection at early stages of infection and reactivation","authors":"S. Valdebenito-Silva,&nbsp;E. Eugenin,&nbsp;A. Ono","doi":"10.1016/j.jve.2024.100454","DOIUrl":"10.1016/j.jve.2024.100454","url":null,"abstract":"","PeriodicalId":17552,"journal":{"name":"Journal of Virus Eradication","volume":"10 ","pages":"Pages 42-43"},"PeriodicalIF":3.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143181425","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PP1.17 – 00054 Optimizing Detection of HIV-1 Infected Cells: A Novel Bioinformatics Pipeline Leveraging Kraken for Single-Cell Multiomics Datasets","authors":"L. Garrido-Sanz,&nbsp;L.B. Soriaga,&nbsp;E. Wong,&nbsp;M.C. Puertas,&nbsp;J. Dalmau,&nbsp;P. Coll,&nbsp;B. Mothe,&nbsp;B. Clotet,&nbsp;A. Telenti,&nbsp;J. Martinez-Picado,&nbsp;S. Morón-López","doi":"10.1016/j.jve.2024.100466","DOIUrl":"10.1016/j.jve.2024.100466","url":null,"abstract":"","PeriodicalId":17552,"journal":{"name":"Journal of Virus Eradication","volume":"10 ","pages":"Page 51"},"PeriodicalIF":3.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143181384","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PP2.2 – 00177 Late Increases in Intact Proviral DNA Reveal Variable HIV-1 Reservoir Dynamics","authors":"L. Brandt,&nbsp;S. Patro,&nbsp;W. Wang,&nbsp;N. Mckenna,&nbsp;E. Halvas,&nbsp;J. Cyktor,&nbsp;R.T. Gandhi,&nbsp;J. Eron,&nbsp;R. Bosch,&nbsp;M. Kearney,&nbsp;D. Mcmahon,&nbsp;J. Mellors","doi":"10.1016/j.jve.2024.100472","DOIUrl":"10.1016/j.jve.2024.100472","url":null,"abstract":"","PeriodicalId":17552,"journal":{"name":"Journal of Virus Eradication","volume":"10 ","pages":"Pages 54-55"},"PeriodicalIF":3.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143180588","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}