Journal of Virus Eradication最新文献

{"title":"Development of a latency model for HIV-1 subtype C and the impact of long terminal repeat element genetic variation on latency reversal","authors":"Shreyal Maikoo ,&nbsp;Robert-Jan Palstra ,&nbsp;Krista L. Dong ,&nbsp;Tokameh Mahmoudi ,&nbsp;Thumbi Ndung'u ,&nbsp;Paradise Madlala","doi":"10.1016/j.jve.2024.100575","DOIUrl":"10.1016/j.jve.2024.100575","url":null,"abstract":"<div><div>Sub-Saharan Africa accounts for almost 70 % of people living with HIV (PLWH) worldwide, with the greatest numbers centred in South Africa where 98 % of infections are caused by subtype C (HIV-1C). However, HIV-1 subtype B (HIV-1B), prevalent in Europe and North America, has been the focus of most cure research and testing despite making up only 12 % of HIV-1 infections globally. Development of latency models for non-subtype B viruses is a necessary step to address this disproportionate focus. Furthermore, the impact of genetic variation between viral subtypes, specifically within the long terminal repeat (LTR) element of the viral transcriptional promoter on latency reversal, remains unclear. To address this scientific gap, we constructed a minimal genome retroviral vector expressing HIV-1C consensus transactivator of transcription protein (Tat) and green fluorescent protein (GFP) under the control of either HIV-1C consensus LTR (C731CC) or the transmitted/founder (T/F) LTRs derived from PLWH (C_T/F731CC), produced corresponding LTR pseudotyped viruses using a vesicular stomatitis virus (VSV-G) pseudotyped Envelope vector and the pCMVΔR8.91 packaging vector containing HIV-1 accessory and <em>rev</em> genes. Viruses produced in this way were used to infect Jurkat E6 and primary CD4⁺ T cells <em>in vitro</em>. By enriching for latently infected cells, and treating them with different latency reversing agents, we developed an HIV-1C latency model that demonstrated that the HIV-1C consensus LTR has lower reactivation potential compared to its HIV-1B counterpart. Furthermore, HIV-1C T/F LTR pseudotyped proviral genetic variants exhibited a heterogenous reactivation response which was modulated by host cell (genetic) variation. Our data suggests that genetic variation both within and between HIV-1 subtypes influences latency reversal. Future studies should investigate the specific role of variation in host cellular environment on reactivation differences.</div></div>","PeriodicalId":17552,"journal":{"name":"Journal of Virus Eradication","volume":"10 4","pages":"Article 100575"},"PeriodicalIF":3.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11730875/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142983723","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"6.3 – 00019 AAV Delivery of the CCR5-blocking monoclonal antibody Leronlimab yields long-term expression and ART-free remission from SHIV viremia","authors":"H.L. Wu,&nbsp;G.M. Webb,&nbsp;J. Zikos,&nbsp;D.M. Magnani,&nbsp;S.P. Fuchs,&nbsp;R.C. Desrosiers,&nbsp;J.B. Sacha","doi":"10.1016/j.jve.2024.100426","DOIUrl":"10.1016/j.jve.2024.100426","url":null,"abstract":"","PeriodicalId":17552,"journal":{"name":"Journal of Virus Eradication","volume":"10 ","pages":"Pages 21-22"},"PeriodicalIF":3.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143128837","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"6.4 – 00112 Overcoming immune responses directed toward AAV-delivered bNAbs","authors":"M. Kuipa,&nbsp;P. Koroma,&nbsp;I. Leguizamo,&nbsp;P. Dhole,&nbsp;M.R. Gardner","doi":"10.1016/j.jve.2024.100427","DOIUrl":"10.1016/j.jve.2024.100427","url":null,"abstract":"","PeriodicalId":17552,"journal":{"name":"Journal of Virus Eradication","volume":"10 ","pages":"Pages 22-23"},"PeriodicalIF":3.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143130657","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PP3.1 – 00160 Macrophage Training Reactivates Latent HIV-1 from HAARTsuppressed PBMCs of PLWH","authors":"S. John,&nbsp;H. James,&nbsp;C. Perritano,&nbsp;I. Fraser","doi":"10.1016/j.jve.2024.100482","DOIUrl":"10.1016/j.jve.2024.100482","url":null,"abstract":"","PeriodicalId":17552,"journal":{"name":"Journal of Virus Eradication","volume":"10 ","pages":"Pages 59-61"},"PeriodicalIF":3.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143134581","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PP3.2 – 00156 Endogenous Cytokine Reporter Macrophages Facilitate Identification of Novel Training Compounds that can Reactivate and Initiate Clearance of Latent HIV","authors":"S.P. John,&nbsp;D. Dabral,&nbsp;C.M. Perritano,&nbsp;H. James,&nbsp;J. Marugan,&nbsp;M. Henderson,&nbsp;I.D.C. Fraser","doi":"10.1016/j.jve.2024.100483","DOIUrl":"10.1016/j.jve.2024.100483","url":null,"abstract":"","PeriodicalId":17552,"journal":{"name":"Journal of Virus Eradication","volume":"10 ","pages":"Page 61"},"PeriodicalIF":3.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143134582","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"2.2 – 00058 Role of HIV integration site on clonal expansion of infected cells and maintenance of latency in vivo","authors":"V. Pal,&nbsp;M. Frauke,&nbsp;A. Danesh,&nbsp;M. Canis,&nbsp;T. Dilling,&nbsp;I. Miller,&nbsp;T. Huynh,&nbsp;T. Hatziioannou,&nbsp;R.B. Jones,&nbsp;G.Q. Lee,&nbsp;P.D. Bieniasz","doi":"10.1016/j.jve.2024.100401","DOIUrl":"10.1016/j.jve.2024.100401","url":null,"abstract":"","PeriodicalId":17552,"journal":{"name":"Journal of Virus Eradication","volume":"10 ","pages":"Page 5"},"PeriodicalIF":3.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143128930","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"8.4 – 00081 IL-15/IL-15RA Therapy Enhances Control of Viral Rebound in SIVInfected Macaques","authors":"S. Govindaraj,&nbsp;H. Babu,&nbsp;S. Ali,&nbsp;S.A. Rahman,&nbsp;S.P. Ribeiro,&nbsp;J. Tomalka,&nbsp;A. Sharma,&nbsp;R.P. Sekaly,&nbsp;F. Villinger,&nbsp;R.R. Amara,&nbsp;V. Velu","doi":"10.1016/j.jve.2024.100439","DOIUrl":"10.1016/j.jve.2024.100439","url":null,"abstract":"","PeriodicalId":17552,"journal":{"name":"Journal of Virus Eradication","volume":"10 ","pages":"Pages 30-31"},"PeriodicalIF":3.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143129007","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"8.3 – 00088 Epitope-Driven Effector Functions of Broadly Neutralizing Antibodies Across Diverse HIV Isolates: Insights for Next-Generation Therapeutics","authors":"C. Li,&nbsp;M. Phelps,&nbsp;J.M. Brady,&nbsp;A.D. Nitido,&nbsp;V. Okonkwo,&nbsp;D. Lingwood,&nbsp;A.B. Balazs","doi":"10.1016/j.jve.2024.100438","DOIUrl":"10.1016/j.jve.2024.100438","url":null,"abstract":"","PeriodicalId":17552,"journal":{"name":"Journal of Virus Eradication","volume":"10 ","pages":"Page 30"},"PeriodicalIF":3.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143129008","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"8.1 – 00022 Short-term Combination Immunotherapy with bNAbs and CCR5 Blockade Mediates ART-Free Viral Control in Infant Rhesus Macaques","authors":"N.L. Haigwood,&nbsp;T. Ordonez,&nbsp;S. Pandey,&nbsp;J. Reed,&nbsp;G.M. Webb,&nbsp;A.J. Hessell,&nbsp;K.K.A. Van Rompay,&nbsp;J.K. Watanabe,&nbsp;J.L. Usachenko,&nbsp;J. Sacha","doi":"10.1016/j.jve.2024.100436","DOIUrl":"10.1016/j.jve.2024.100436","url":null,"abstract":"","PeriodicalId":17552,"journal":{"name":"Journal of Virus Eradication","volume":"10 ","pages":"Pages 28-29"},"PeriodicalIF":3.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143130231","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PP2.2 – 00177 Late Increases in Intact Proviral DNA Reveal Variable HIV-1 Reservoir Dynamics","authors":"L. Brandt,&nbsp;S. Patro,&nbsp;W. Wang,&nbsp;N. Mckenna,&nbsp;E. Halvas,&nbsp;J. Cyktor,&nbsp;R.T. Gandhi,&nbsp;J. Eron,&nbsp;R. Bosch,&nbsp;M. Kearney,&nbsp;D. Mcmahon,&nbsp;J. Mellors","doi":"10.1016/j.jve.2024.100472","DOIUrl":"10.1016/j.jve.2024.100472","url":null,"abstract":"","PeriodicalId":17552,"journal":{"name":"Journal of Virus Eradication","volume":"10 ","pages":"Pages 54-55"},"PeriodicalIF":3.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143180588","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}