Journal of Vascular Research最新文献_第3页

Autophagy Induced by Low Shear Stress Leads to Endothelial Glycocalyx Disruption. 低剪切应力诱导的自噬导致内皮细胞糖萼破坏

IF 1.7 4区医学

Journal of Vascular Research Pub Date : 2024-01-01 Epub Date: 2024-03-19 DOI: 10.1159/000537772

Lina Lin, Wei Gao, Linya Feng, Chundong Wang, Ruiqi Yang, Weijian Wang, Qiaolin Wu

{"title":"Autophagy Induced by Low Shear Stress Leads to Endothelial Glycocalyx Disruption.","authors":"Lina Lin, Wei Gao, Linya Feng, Chundong Wang, Ruiqi Yang, Weijian Wang, Qiaolin Wu","doi":"10.1159/000537772","DOIUrl":"10.1159/000537772","url":null,"abstract":"Introduction: Previous studies have confirmed that low shear stress (LSS) induces glycocalyx disruption, leading to endothelial dysfunction. However, the role of autophagy in LSS-induced glycocalyx disruption and relevant mechanism are not clear. In this study, we hypothesized that LSS may promote autophagy, disrupting the endothelium glycocalyx.Methods: Human umbilical vein endothelial cells were subjected to physiological shear stress and LSS treatments, followed by the application of autophagy inducers and inhibitors. Additionally, cells were treated with specific matrix metalloproteinase-2 (MMP-2) and matrix metalloproteinase-9 (MMP-9) inhibitor. The expression of autophagic markers, glycocalyx, MMP-2, and MMP-9 was measured.Results: LSS impacted the expression of endothelium autophagy markers, increasing the expression of LC3II.LC3I-1 and Beclin-1, and decreasing the levels of p62, accompanied by glycocalyx disturbance. Moreover, LSS upregulated the expression of MMP-2 and MMP-9 and downregulated the levels of syndecan-1 and heparan sulfate (HS). Additionally, expression of MMP-2 and MMP-9 was increased by an autophagy promoter but was decreased by autophagy inhibitor treatment under LSS. Autophagy and MMP-2 and MMP-9 further caused glycocalyx disruption.Conclusion: LSS promotes autophagy, leading to glycocalyx disruption. Autophagy increases the expression of MMP-2 and MMP-9, which are correlated with the glycocalyx destruction induced by LSS.","PeriodicalId":17530,"journal":{"name":"Journal of Vascular Research","volume":" ","pages":"77-88"},"PeriodicalIF":1.7,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140175185","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Preserving Endothelial Integrity in Human Saphenous Veins during Preparation for Coronary Bypass Surgery. 在准备冠状动脉搭桥手术期间保持人体无隐静脉内皮的完整性。

IF 1.7 4区医学

Journal of Vascular Research Pub Date : 2024-01-01 Epub Date: 2024-01-12 DOI: 10.1159/000535843

Meghan W Sedovy, Xinyan Leng, Farwah Iqbal, Mark C Renton, Melissa Leaf, Kailynn Roberts, Arya Malek, W Scott Arnold, David A Wyatt, Cynthia W Choate, Joseph F Rowe, Joseph W Baker, Scott R Johnstone, Mark Joseph

{"title":"Preserving Endothelial Integrity in Human Saphenous Veins during Preparation for Coronary Bypass Surgery.","authors":"Meghan W Sedovy, Xinyan Leng, Farwah Iqbal, Mark C Renton, Melissa Leaf, Kailynn Roberts, Arya Malek, W Scott Arnold, David A Wyatt, Cynthia W Choate, Joseph F Rowe, Joseph W Baker, Scott R Johnstone, Mark Joseph","doi":"10.1159/000535843","DOIUrl":"10.1159/000535843","url":null,"abstract":"Introduction: While multiple factors influence coronary artery bypass graft (CABG) success rates, preserving saphenous vein endothelium during surgery may improve patency. Standard preparations include saphenous vein preparation in heparinized saline (saline) which can result in endothelial loss and damage. Here, we investigated the impact of preparing saphenous graft vessels in heparinized patient blood (blood) versus saline.Methods: Saphenous vein tissues from a total of 23 patients undergoing CABG were split into 2 groups (1) saline and (2) heparinized patient blood. Excess tissue was fixed for analysis immediately following surgery. Level of endothelial coverage, oxidative stress marker 4-hydroxynonenal (4HNE), and oxidative stress protective marker nuclear factor erythroid 2-related factor 2 (NRF2) were evaluated.Results: In saline patient veins, histological analysis revealed a limited luminal layer, suggesting a loss of endothelial cells (ECs). Immunofluorescent staining of EC markers vascular endothelial cadherin (VE-cadherin) and endothelial nitric oxide identified a significant improvement in EC coverage in the blood versus saline groups. Although both treatment groups expressed 4HNE to similar levels, EC blood samples expressed higher levels of NRF2.Conclusion: Our data indicate that use of heparinized patient blood helps preserve the endothelium and promotes vein graft health. This has the potential to improve long-term outcomes in patients.","PeriodicalId":17530,"journal":{"name":"Journal of Vascular Research","volume":" ","pages":"68-76"},"PeriodicalIF":1.7,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139466636","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nitric Oxide/Glucose Transporter Type 4 Pathway Mediates Cardioprotection against Ischemia/Reperfusion Injury under Hyperglycemic and Diabetic Conditions in Rats. 一氧化氮/葡萄糖转运体 4 型通路介导大鼠在高血糖和糖尿病条件下对缺血/再灌注损伤的心脏保护作用

IF 1.8 4区医学

Journal of Vascular Research Pub Date : 2024-01-01 Epub Date: 2024-06-28 DOI: 10.1159/000539461

Aisha Al-Kouh, Fawzi Babiker

{"title":"Nitric Oxide/Glucose Transporter Type 4 Pathway Mediates Cardioprotection against Ischemia/Reperfusion Injury under Hyperglycemic and Diabetic Conditions in Rats.","authors":"Aisha Al-Kouh, Fawzi Babiker","doi":"10.1159/000539461","DOIUrl":"10.1159/000539461","url":null,"abstract":"Introduction: The comorbidities of ischemic heart disease (IHD) and diabetes mellitus (DM) compromise the protection of the diabetic heart from ischemia/reperfusion (I/R) injury. We hypothesized that manipulation of reperfusion injury salvage kinase (RISK) and survivor activating factor enhancement (SAFE) pathways might protect the diabetic heart, and intervention of these pathways could be a new avenue for potentially protecting the diabetic heart.Methods: All hearts were subjected to 30-min ischemia and 30-min reperfusion. During reperfusion, hearts were exposed to molecules proven to protect the heart from I/R injury. The hemodynamic data were collected using suitable software. The infarct size, troponin T levels, and protein levels in hearts were evaluated.Results: Both cyclosporine-A and nitric oxide donor (SNAP) infusion at reperfusion protected 4-week diabetic hearts from I/R injury. However, 6-week diabetic hearts were protected only by SNAP, but not cyclosporin-A. These treatments significantly (p < 0.05) improved cardiac hemodynamics and decreased infarct size.Conclusions: The administration of SNAP to diabetic hearts protected both 4- and 6-week diabetic hearts; however, cyclosporine-A protected only the 4-week diabetic hearts. The eNOS/GLUT-4 pathway executed the SNAP-mediated cardioprotection.","PeriodicalId":17530,"journal":{"name":"Journal of Vascular Research","volume":" ","pages":"179-196"},"PeriodicalIF":1.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141476895","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Regulation of Skeletal Muscle Resistance Arteriolar Tone: Temporal Variability in Vascular Responses. 骨骼肌阻力动脉张力的调节：血管反应的时空变异性。

IF 1.8 4区医学

Journal of Vascular Research Pub Date : 2024-01-01 Epub Date: 2024-10-03 DOI: 10.1159/000541169

Brayden D Halvorson, Aaron D Ward, Donna Murrell, James C Lacefield, Robert W Wiseman, Daniel Goldman, Jefferson C Frisbee

{"title":"Regulation of Skeletal Muscle Resistance Arteriolar Tone: Temporal Variability in Vascular Responses.","authors":"Brayden D Halvorson, Aaron D Ward, Donna Murrell, James C Lacefield, Robert W Wiseman, Daniel Goldman, Jefferson C Frisbee","doi":"10.1159/000541169","DOIUrl":"10.1159/000541169","url":null,"abstract":"Introduction: A full understanding of the integration of the mechanisms of vascular tone regulation requires an interrogation of the temporal behavior of arterioles across vasoactive challenges. Building on previous work, the purpose of the present study was to start to interrogate the temporal nature of arteriolar tone regulation with physiological stimuli.Methods: We determined the response rate of ex vivo proximal and in situ distal resistance arterioles when challenged by one-, two-, and three-parameter combinations of five major physiological stimuli (norepinephrine, intravascular pressure, oxygen, adenosine [metabolism], and intralumenal flow). Predictive machine learning models determined which factors were most influential in controlling the rate of arteriolar responses.Results: Results indicate that vascular response rate is dependent on the intensity of the stimulus used and can be severely hindered by altered environments, caused by application of secondary or tertiary stimuli. Advanced analytics suggest that adrenergic influences were dominant in predicting proximal arteriolar response rate compared to metabolic influences in distal arterioles.Conclusion: These data suggest that the vascular response rate to physiologic stimuli can be strongly influenced by the local environment. Translating how these effects impact vascular networks is imperative for understanding how the microcirculation appropriately perfuses tissue across conditions.","PeriodicalId":17530,"journal":{"name":"Journal of Vascular Research","volume":" ","pages":"269-297"},"PeriodicalIF":1.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11652243/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142372206","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cerebral Cortical Vasodilation via Nicotinic Receptors by Heated Tobacco Product Aerosol Extract in Rats. 加热烟草制品气溶胶提取物通过尼古丁受体对大鼠大脑皮层血管的扩张作用

IF 1.8 4区医学

Journal of Vascular Research Pub Date : 2024-01-01 Epub Date: 2024-11-05 DOI: 10.1159/000541726

Sae Uchida, Jura Moriya, Daichi Morihara, Mayura Shimura, Fusako Kagitani

{"title":"Cerebral Cortical Vasodilation via Nicotinic Receptors by Heated Tobacco Product Aerosol Extract in Rats.","authors":"Sae Uchida, Jura Moriya, Daichi Morihara, Mayura Shimura, Fusako Kagitani","doi":"10.1159/000541726","DOIUrl":"10.1159/000541726","url":null,"abstract":"Introduction: Smoking increases the risk of lung cancer due to a number of components of smoke. The use of novel heated tobacco products (HTPs), alternative to conventional combustion cigarettes, has increased in recent years. However, the in vivo biological effects of HTPs are poorly understood. This study aimed to clarify the acute effects of injecting aerosol extract prepared from an HTP on regional cerebral blood flow (rCBF) in rat cortex by comparing them to the effects of injecting smoke extract prepared from conventional combustible cigarettes.Methods: In urethane anesthetized rats, rCBF was measured using laser speckle contrast imaging simultaneously with arterial pressure.Results: Both cigarette smoke extract and HTP aerosol extract, at a dose equivalent to 30 μg nicotine/kg, injected intravenously, increased cortical rCBF without changing arterial pressure. The magnitude and time course of the increased rCBF response to both extracts were similar throughout the cortical area, and the rCBF increases were all abolished by dihydro-β-erythroidine, an α4β2-preferring nicotinic acetylcholine receptor (nAChR) antagonist.Conclusion: In conclusion, our study demonstrated that the effect of injecting aerosol extract prepared from an HTP, an acute increase in cortical rCBF, is mediated via activation of α4β2-like neuronal nAChRs in the brain.","PeriodicalId":17530,"journal":{"name":"Journal of Vascular Research","volume":" ","pages":"318-326"},"PeriodicalIF":1.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11651226/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142583763","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bone Morphogenetic Protein-4 Promotes Phenotypic Modulation via SMAD-4/MCT-4 Axis in Vascular Smooth Muscle Cells. 骨形态发生蛋白-4 通过 SMAD-4/MCT-4 轴促进血管平滑肌细胞的表型调节

IF 1.8 4区医学

Journal of Vascular Research Pub Date : 2024-01-01 Epub Date: 2023-12-27 DOI: 10.1159/000532029

Qi Li, Zhongsha Li, Jingyu Li, Xiaoling Qin, Fengjiao Wu, Chang Chen

{"title":"Bone Morphogenetic Protein-4 Promotes Phenotypic Modulation via SMAD-4/MCT-4 Axis in Vascular Smooth Muscle Cells.","authors":"Qi Li, Zhongsha Li, Jingyu Li, Xiaoling Qin, Fengjiao Wu, Chang Chen","doi":"10.1159/000532029","DOIUrl":"10.1159/000532029","url":null,"abstract":"Introduction: This study aimed to determine whether bone morphogenetic protein-4 (BMP-4), which increases in response to intimal hyperplasia, promotes phenotype transition in vascular smooth muscle cells (VSMCs).Methods: Balloon injury was used to induce intimal hyperplasia in rats. Hematoxylin-eosin staining was used to detect the alteration of vascular structure. Serum levels of BMP-4 and lactate were detected by ELISA. Human aortic smooth muscle cells (HA-SMCs) were cultured. Protein and mRNA expression levels were detected through Western blot and real-time PCR. Cell migration was measured by transwell assay.Results: Our data showed that serum concentration of BMP-4 was upregulated after balloon injury. Treatment with BMP-4 inhibitor DMH1 (4-(6-(4-isopropoxyphenyl)pyrazolo(1,5-a)pyrimidin-3-yl)quinoline) suppressed the abnormal expression of BMP-4 and inhibited the intimal hyperplasia induced by balloon injury. Compared to BMP-4-negative medium, BMP-4-positive medium was associated with higher synthetic VSMC marker expression levels and lower in contractile gene markers in cultured HA-SMCs. Transfection of monocarboxylic acid transporters-4 (MCT-4) siRNA inhibited the excretion of lactate induced by BMP-4.Conclusion: Our analyses provided evidence that BMP-4 and its regulator Smad-4 are key regulators in MCT-4-mediated lactate excretion. This indicates that BMP-4 stimulates the phenotypic transition of VSMCs via SMAD-4/MCT-4 signaling pathway.","PeriodicalId":17530,"journal":{"name":"Journal of Vascular Research","volume":" ","pages":"99-108"},"PeriodicalIF":1.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139048992","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Preoperative Congestive Heart Failure Is Associated with Higher 30-Day Myocardial Infarction and Pneumonia after Endovascular Repair of Abdominal Aortic Aneurysm. 腹主动脉瘤血管内修复术后 30 天心肌梗死和肺炎发生率较高与术前充血性心力衰竭有关。

IF 1.8 4区医学

Journal of Vascular Research Pub Date : 2024-01-01 Epub Date: 2024-09-19 DOI: 10.1159/000540918

Renxi Li, Anton Sidawy, Bao-Ngoc Nguyen

{"title":"Preoperative Congestive Heart Failure Is Associated with Higher 30-Day Myocardial Infarction and Pneumonia after Endovascular Repair of Abdominal Aortic Aneurysm.","authors":"Renxi Li, Anton Sidawy, Bao-Ngoc Nguyen","doi":"10.1159/000540918","DOIUrl":"10.1159/000540918","url":null,"abstract":"Introduction: Preoperative congestive heart failure (CHF) is associated with higher postoperative mortality and complications in noncardiac surgery. However, postoperative outcomes for patients with preoperative CHF undergoing endovascular aneurysm repair (EVAR) have not been thoroughly established. This study evaluated the effect of preoperative CHF on 30-day outcomes following nonemergent intact EVAR using a large-scale national registry.Methods: Patients who had infrarenal EVAR were identified in the ACS-NSQIP database from 2012 to 2022. A 1:5 propensity-score matching was used to match demographics, baseline characteristics, aneurysm diameter, distant aneurysm extent, anesthesia, and concomitant procedures between patients with and without preoperative CHF. Thirty-day postoperative outcomes were examined.Results: 467 (2.84%) CHF patients underwent intact EVAR. Meanwhile, 15,996 non-CHF patients underwent EVAR, where 2,248 of them were matched to all CHF patients. Patients with and without preoperative CHF had comparable 30-day mortality (3.02% vs. 2.62%, p = 0.64). However, CHF patients had higher myocardial infarction (3.02% vs. 1.47%, p = 0.03), pneumonia (3.23% vs. 1.73%, p = 0.04), 30-day readmission (p = 0.01), and longer length of stay (p < 0.01).Conclusion: While patients with and without preoperative CHF had comparable 30-day mortality rates, those with CHF faced higher risks of cardiopulmonary complications. Effective management of preoperative CHF may help prevent postoperative complications in these patients.","PeriodicalId":17530,"journal":{"name":"Journal of Vascular Research","volume":" ","pages":"225-232"},"PeriodicalIF":1.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142290067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Relationship of Warfarin and Apixaban with Vascular Function in Patients with Atrial Fibrillation. 华法林和阿哌沙班与心房颤动患者血管功能的关系

IF 1.7 4区医学

Journal of Vascular Research Pub Date : 2024-01-01 Epub Date: 2024-03-06 DOI: 10.1159/000535618

Rehan T Junejo, Dhiraj Gupta, Richard L Snowdon, Gregory Y H Lip, James P Fisher

{"title":"Relationship of Warfarin and Apixaban with Vascular Function in Patients with Atrial Fibrillation.","authors":"Rehan T Junejo, Dhiraj Gupta, Richard L Snowdon, Gregory Y H Lip, James P Fisher","doi":"10.1159/000535618","DOIUrl":"10.1159/000535618","url":null,"abstract":"Introduction: Atrial fibrillation (AF) is associated with endothelial damage/dysfunction. Herein, we tested the hypothesis that brachial artery flow-mediated dilation (FMD) is superior in AF patients taking apixaban compared to warfarin.Methods: AF patients on apixaban (n = 46; 67 [7] years; mean [standard deviation]; 15 women) and warfarin (n = 27; 73 [9] years (p < 0.01); 11 women) were recruited. Duplex Doppler ultrasound imaging was undertaken during baseline (2 min), cuff inflation (5 min), and following cuff deflation (3 min). FMD was defined as peak increase in brachial artery diameter following cuff deflation and analysed as percentage change in diameter, as a ratio of FMD, shear rate area under the curve (SRAUC; FMD-to-SRAUC), and using SRAUC as a covariate (FMDSR).Results: Baseline artery diameter (4.96 [1.14] vs. 4.89 [0.88] mm), peak diameter (5.12 [1.17] vs. 5.14 [0.93] mm), and FMDSR (3.89 [3.62] vs. 4.80 [3.60] %) were not different between warfarin and apixaban (p > 0.05; analysis of covariance with age, CHA2DS2-VASc, years since AF diagnosis, number of diabetics, alcohol drinkers, and units of alcohol consumed per week as covariates). Stepwise multiple regression identified independent association of fibrillation, hypertension, and increased age with FMD.Conclusion: AF patients on warfarin and apixaban exhibit similar endothelium-dependent vasodilation. Increased blood pressure negatively impacts vasodilator capacity in AF patients.","PeriodicalId":17530,"journal":{"name":"Journal of Vascular Research","volume":" ","pages":"59-67"},"PeriodicalIF":1.7,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10997243/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140049792","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Perivascular Adipose Tissue Remodels Only after Elevation of Blood Pressure in the Dahl SS Rat Fed a High-Fat Diet. 以高脂肪饮食喂养的 Dahl SS 大鼠只有在血压升高后血管周围脂肪组织才会发生重塑。

IF 1.7 4区医学

Journal of Vascular Research Pub Date : 2024-01-01 Epub Date: 2023-12-19 DOI: 10.1159/000535513

Caitlin Wilson, Janice M Thompson, Leah Terrian, Adam D Lauver, Emma D Flood, Gregory D Fink, Lisa Sather, Sudin Bhattacharya, G Andres Contreras, Stephanie W Watts

{"title":"Perivascular Adipose Tissue Remodels Only after Elevation of Blood Pressure in the Dahl SS Rat Fed a High-Fat Diet.","authors":"Caitlin Wilson, Janice M Thompson, Leah Terrian, Adam D Lauver, Emma D Flood, Gregory D Fink, Lisa Sather, Sudin Bhattacharya, G Andres Contreras, Stephanie W Watts","doi":"10.1159/000535513","DOIUrl":"10.1159/000535513","url":null,"abstract":"Introduction: Tunica media extracellular matrix (ECM) remodeling is well understood to occur in response to elevated blood pressure, unlike the remodeling of other tunicas. We hypothesize that perivascular adipose tissue (PVAT) is responsive to hypertension and remodels as a protective measure.Methods: The adventitia and PVAT of the thoracic aorta were used in measuring ECM genes from 5 pairs of Dahl SS male rats on 8 or 24 weeks of feeding from weaning on a control (10% Kcal fat) or high-fat (HF; 60%) diet. A PCR array of ECM genes was performed with cDNA from adventitia and PVAT after 8 and 24 weeks. A gene regulatory network of the differentially expressed genes (DEGs) (HF 2-fold > con) was created using Cytoscape.Results: After 8 weeks, 29 adventitia but 0 PVAT DEGs were found. By contrast, at 24 weeks, PVAT possessed 47 DEGs while adventitia had 3. Top DEGs at 8 weeks in adventitia were thrombospondin 1 and collagen 8a1. At 24 weeks, thrombospondin 1 was also a top DEG in PVAT. The transcription factor Adarb1 was identified as a regulator of DEGs in 8-week adventitia and 24-week PVAT.Conclusion: These data support that PVAT responds biologically once blood pressure is elevated.","PeriodicalId":17530,"journal":{"name":"Journal of Vascular Research","volume":" ","pages":"26-37"},"PeriodicalIF":1.7,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10836923/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138805883","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Retraction Statement. 撤回声明。

IF 1.8 4区医学

Journal of Vascular Research Pub Date : 2024-01-01 Epub Date: 2024-07-19 DOI: 10.1159/000540333

引用次数: 0