Journal of Traditional Chinese Medicine最新文献

{"title":"Exploring pathogenesis in subjects with subjective Tinnitus having kidney deficiency pattern in terms of Traditional Chinese Medicine based on serum metabolic profiles","authors":"Tan Yong ,&nbsp;Shen Shanshan ,&nbsp;Guan Fulan ,&nbsp;He Bing ,&nbsp;Lu Cheng ,&nbsp;Xiao Cheng ,&nbsp;Jiang Miao ,&nbsp;Zhao Ning ,&nbsp;Li Li ,&nbsp;Cheng Shiping ,&nbsp;Zu Xianpeng ,&nbsp;Zhang Weidong ,&nbsp;Liu Xinru ,&nbsp;Lü Aiping","doi":"10.1016/S0254-6272(18)30918-X","DOIUrl":"10.1016/S0254-6272(18)30918-X","url":null,"abstract":"<div><h3>OBJECTIVE</h3><p>To investigate the metabolic pathogenesis in subjects with subjective tinnitus (ST) having kidney deficiency pattern (KDP) (ST/KDP) in terms of Traditional Chinese Medicine.</p></div><div><h3>METHODS</h3><p>Three groups of subjects, including healthy individuals, subjects with ST/KDP, and subjects who were healthy initially and then developed ST/KDP one year later (healthy → ST/KDP), were recruited for this study. Serum metabolic profiles of all subjects were analyzed using ultra-performance liquid chromatography coupled with quadruple-time-of-flight mass spectrometry. The metabolic characteristics of the ST/KDP subjects were determined, and the corresponding biomarkers were predicted. The metabolomics data from the healthy → ST/KDP subjects were collected for further verification.</p></div><div><h3>RESULTS</h3><p>Twelve metabolites in the ST/KDP subjects were different from those of the healthy control subjects. Of these metabolites, according to the prediction, except for octanoic acid, other metabolites might characterize ST/KDP. Ten metabolites at the outcome ST/KDP stage were different from those at the initial (control) stage. Through the comparison of these metabolites with the predicted metabolites, five common metabolites, including upregulated glutamate, serotonin, orotic acid and 8-oxoguanine, as well as downregulated taurine, were found. These common metabolites were significantly associated with canonical pathways including calcium signaling, γ-aminobutyric acid (GABA) receptor signaling, purine and pyrimidine biosynthesis, taurine biosynthesis, and serotonin receptor signaling.</p></div><div><h3>CONCLUSION</h3><p>The metabolic pathogenesis in ST/KDP subjects was characterized by upregulated glutamate, serotonin, orotic acid and 8-oxoguanine, as well as downregulated taurine, additionally, perturbations of calcium signaling, GABA receptor signaling, purine and pyrimidine biosynthesis, taurine biosynthesis, and serotonin receptor signaling.</p></div>","PeriodicalId":17513,"journal":{"name":"Journal of Traditional Chinese Medicine","volume":"38 5","pages":"Pages 773-780"},"PeriodicalIF":2.6,"publicationDate":"2018-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0254-6272(18)30918-X","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85539195","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Weipixiao ameliorates gastric precancerous lesions in a rat's model by regulating GSK3β and C-myc","authors":"Zeng Jinhao ,&nbsp;Pan Huafeng ,&nbsp;Guo Jing ,&nbsp;Gong Daoyin ,&nbsp;Cai Tiantian ,&nbsp;Chen Xiaodong ,&nbsp;Zhang Yi ,&nbsp;You Fengming ,&nbsp;Chen Longhui ,&nbsp;Zhao Ziming ,&nbsp;Liang Chao","doi":"10.1016/S0254-6272(18)30909-9","DOIUrl":"10.1016/S0254-6272(18)30909-9","url":null,"abstract":"<div><h3>OBJECTIVE</h3><p>To investigate the mechanism underlying the action of Weipixiao (WPX) in a rat's model with ameliorating gastric precancerous lesions (GPL).</p></div><div><h3>METHODS</h3><p>HPLC analysis was performed to identify the chemical constituents of WPX preparation. Sprague- Dawley rats were randomly assigned into control group, model group, vitacoenzyme group, high-dose WPX group (H-WPX), medium-dose WPX group (M-WPX) and low-dose WPX group (L-WPX). After modeling, the treated rats were administrated WPX or vitacoenzyme intragastrically for consecutive 10 weeks. Gene and protein expressions of GSK3β, C-myc, Cylin E were evaluated by quantitative real-time reverse transcription-polymerase chain reaction (RT-qPCR) and immunohistochemistry, respectively.</p></div><div><h3>RESULTS</h3><p>WPX could efficiently attenuate the pathological alterations of “non-progressive GPL” in rats. As expected, mRNA and protein levels of C-myc and Cylin E were up-regulated in model rats, while GSK3β expression down-regulated (<em>P</em> &lt; 0.01). WPX treatment, especially at low dose, could significantly down-regulate the mRNA as well as protein levels of C-myc, and could lead to remarkable up-regulation of mRNA and protein levels of GSK3β in GPL rats (<em>P</em> &lt; 0.05). However, no significant changes were observed in WPX-treated rats.</p></div><div><h3>CONCLUSION</h3><p>Our findings suggested that WPX-mediated attenuation of GPL pathological alterations might be due to its regulatory effect on the expressions of GSK3β and C-myc, and on the dysregulation of Wnt/GSK3β pathway.</p></div>","PeriodicalId":17513,"journal":{"name":"Journal of Traditional Chinese Medicine","volume":"38 5","pages":"Pages 705-713"},"PeriodicalIF":2.6,"publicationDate":"2018-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0254-6272(18)30909-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41318371","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of the herbal medicine Danggui Sini plus Wuzhuyu Shengjiang Tang on erythrocyte deformability in normal subjects: a cross-over trial","authors":"JiEun Lee ,&nbsp;Seung-Yeon Cho ,&nbsp;Seong-Uk Park ,&nbsp;Woo-Sang Jung ,&nbsp;Sang-Kwan Moon ,&nbsp;Jung-Mi Park ,&nbsp;Chang-Nam Ko ,&nbsp;Ki-Ho Cho ,&nbsp;Seungwon Kwon","doi":"10.1016/S0254-6272(18)30917-8","DOIUrl":"10.1016/S0254-6272(18)30917-8","url":null,"abstract":"<div><h3>OBJECTIVE</h3><p>To show whether Danggui Sini plus Wuzhuyu Shengjiang Tang (DSWST) has any transient effect on erythrocyte deformability in normal subjects.</p></div><div><h3>METHODS</h3><p>A total of 25 subjects [mean age (27.8 ± 1.8) years] was enrolled in this study. The study was designed as a cross-over trial in which the subjects took part for 2 d. On the first day, blood samples were collected at baseline and 1-2 h after administration of water, whereas, on the second day, instead of water, the subjects were administered DSWST after the baseline blood sampling. The blood samples collected at baseline and after the administration water or DSWST, were examined for erythrocyte deformability.</p></div><div><h3>RESULTS</h3><p>The elongation index increased significantly after 2 h (<em>P</em> = 0.009) compared to the baseline after DSWST intake. However, after water intake, there was no significant difference observed. When comparing the percent change of erythrocyte deformability between DSWST and water, we found that after 2 h of administration, DSWST improved erythrocyte deformability significantly compared to water (<em>P</em> &lt; 0.001).</p></div><div><h3>CONCLUSION</h3><p>DSWST has a transient effect on erythrocyte deformability in normal subjects.</p></div>","PeriodicalId":17513,"journal":{"name":"Journal of Traditional Chinese Medicine","volume":"38 5","pages":"Pages 769-772"},"PeriodicalIF":2.6,"publicationDate":"2018-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0254-6272(18)30917-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47814723","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Ginkgo biloba leaf extract on cerebral cortex amino acid levels in cerebral ischemia model rats","authors":"Cui Yiran ,&nbsp;Wu Hongwei ,&nbsp;Liu Mengting ,&nbsp;Qin Haijiao ,&nbsp;Liu Xin ,&nbsp;Yang Hongjun","doi":"10.1016/S0254-6272(18)30906-3","DOIUrl":"10.1016/S0254-6272(18)30906-3","url":null,"abstract":"<div><h3>OBJECTIVE</h3><p>To investigate the effect of Ginkgo biloba leaf extract on amino acid levels in the cerebral cortex of cerebral ischemia model rats induced by middle cerebral artery occlusion (MCAO).</p></div><div><h3>METHODS</h3><p>A rat model of cerebral ischemia was established by MCAO. Male rats were divided into a negative control group (Control), a sham-operated group (Sham), an ischemic group (MCAO), and an ischemic group treated with Ginkgo biloba leaf extract (MCAO_D). All groups were divided into two subgroups with occlusion times of 12 and 24 h, respectively. The levels of 18 endogenous amino acids in the cerebral cortex were quantified by triple quadrupole-liquid chromatography-mass spectrometry.</p></div><div><h3>RESULTS</h3><p>Compared with the MCAO group, behavioral performance, neurological deficit score, and cerebral infarct volume were significantly improved in the MCAO_D group (<em>P</em> &lt; 0.05, <em>P</em> &lt; 0.01). Compared with the sham group, the levels of 17 amino acids in the cerebral cortex were markedly changed in the MCAO group. The levels of Alanine (Ala), Isoleucine (Ile), Glutamic acid (Glu), Serine (Ser), Valine (Val), Phenylalanine (Phe), Proline (Pro), Threonine (Thr), Lysine (Lys), Tyrosine (Tyr), Hydroxyproline (Hyp), Arginine (Arg), Leucine (Leu), Tryptophan (Trp), and Glycine (Gly) were increased (<em>P</em> &lt; 0.001, <em>P</em> &lt; 0.05), while levels of Gln and Tau were decreased (<em>P</em> &lt; 0.001, <em>P</em> &lt; 0.05). Compared with the MCAO group, Ginkgo biloba extract treatment in the MCAO_D group significantly down-regulated the levels of 11 amino acids, especially those of Arg, Thr, and Ser in 12 or 24 h.</p></div><div><h3>CONCLUSION</h3><p>Injection of Ginkgo biloba leaf extract has a therapeutic effect on model rats with MCAO-induced cerebral ischemia by acting on amino acids in the cerebral cortex. This effect might be associated with the regulation of amino acid metabolism in the cerebral cortex.</p></div>","PeriodicalId":17513,"journal":{"name":"Journal of Traditional Chinese Medicine","volume":"38 5","pages":"Pages 676-684"},"PeriodicalIF":2.6,"publicationDate":"2018-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0254-6272(18)30906-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41620552","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Ziyin Jianghuo Ningxin decoction plus dehydroepiandrosterone and femoston in treatment of patients with menopausal symptoms","authors":"Lin Jing ,&nbsp;Zhu Jun ,&nbsp;Tian Fubo ,&nbsp;Chen Lanting ,&nbsp;Zhang Yang ,&nbsp;Wang Yan ,&nbsp;Wang Mingyan ,&nbsp;Hans-Jürgen Gober ,&nbsp;Li Dajin ,&nbsp;Wang Ling","doi":"10.1016/S0254-6272(18)30920-8","DOIUrl":"10.1016/S0254-6272(18)30920-8","url":null,"abstract":"<div><h3>OBJECTIVE</h3><p>To determine the therapeutic effect of Ziyin Jianghuo Ningxin Decoction (ZYJHNXD) plus dehydroepiandrosterone (DHEA) and menopausal hormone therapy (MHT) in patients suffering from menopausal symptoms identified as, in terms of Traditional Chinese Medicine, symptom pattern of <em>Yin</em> deficiency with hyperactive fire.</p></div><div><h3>METHODS</h3><p>Totally 180 postmenopausal women aged 40 to 60 years were assigned into four groups and accepted femoston, femoston with ZYJHNXD, femoston with DHEA, femoston with ZYJHNXD and DHEA therapies, respectively, for three months. Common questionnaire-based measure instruments included modified Kupperman index (MKI), Hamilton Rating Scale for Anxiety (HAMA), and Hamilton Rating Scale for Depression (HAMD). Follicle-stimulating hormone (FSH), luteinizing hormone (LH), estradiol (E2), 5-hydroxyindole-3-acetic acid (5-HIAA), norepinephrine (NE), dopamine (DA), bone mineral density (BMD), and sleep quality were evaluated before and three months after the treatments.</p></div><div><h3>RESULTS</h3><p>In all four groups, the scores of MKI, HAMA, HAMD and the levels of FSH, LH decreased significantly (<em>P</em> &lt; 0.05) after the treatment, while the levels of E2, 5-HIAA, NE, and DA showed obvious elevation (<em>P</em> &lt; 0.05). The group receiving ZYJHNXD and DHEA combined with femoston had superiority in the preservation of bone mineral density and improvement of total sleep time and nighttime sleep time over the other three groups.</p></div><div><h3>CONCLUSION</h3><p>ZYJHNXD and DHEA combined with MHT therapy have a favorable outcome in managing menopausal symptoms, restoring hormone levels, preventing skeletal rarefaction or osteoporosis, and improving sleep quality for postmenopausal women.</p></div>","PeriodicalId":17513,"journal":{"name":"Journal of Traditional Chinese Medicine","volume":"38 5","pages":"Pages 787-796"},"PeriodicalIF":2.6,"publicationDate":"2018-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0254-6272(18)30920-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41914128","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protective effects of effective ingredients of Danshen (Radix Salviae Miltiorrhizae) and Honghua (Flos Carthami) compatibility after rat hippocampal neurons induced by hypoxia injury","authors":"Yu Li ,&nbsp;Wan Haofang ,&nbsp;Jin Weifeng ,&nbsp;Yang Jiehong ,&nbsp;Li Chang ,&nbsp;Dai Liuling ,&nbsp;Ge Lijun ,&nbsp;Zhou Huifen ,&nbsp;Wan Haitong ,&nbsp;He Yu","doi":"10.1016/S0254-6272(18)30907-5","DOIUrl":"10.1016/S0254-6272(18)30907-5","url":null,"abstract":"<div><h3>OBJECTIVE</h3><p>To investigate the effective ingredients of Danshen (<em>Radix Salviae Miltiorrhizae</em>) and Honghua (<em>Flos Carthami</em>) (Danhong) on protective properties towards neonatal rat hippocampal neurons under hypoxia condition.</p></div><div><h3>METHODS</h3><p>Primary culture of neonatal rat hippocampal neurons was used to model hypoxia damage on the hippocampus. Methyl thiazolyl tetrazolium (MTT) assay and preliminary experiments were conducted to identify the four effective ingredients of Danhong, along with the injection of positive drug onto hippocampal neurons at a non-toxic dosage level. The cultured cells were randomly divided into 12 groups: the normal group, model group, positive drug control group and nine compatibility groups of the four effective ingredients. Different test methods were applied to determine lactate dehydrogenasein (LDH), total superoxide dismutase (T-SOD), malondialdehyde (MDA), 6-keto prostaglandin F_la (6-keto-PGF_1a), thromboxane B₂ (TXB₂), mitochondrial membrane potential (MMP), free calcium ions concentration ([Ca²⁺]i) and early onset cell apoptosis.</p></div><div><h3>RESULTS</h3><p>Different compatibility groups could inhibit the content of LDH and intracellular calcium overload, increase activity in T-SOD, decrease level of MDA and TXB₂, improve level of 6-keto-PGF_1a and MMP, and prevent the early onset cell apoptosis.</p></div><div><h3>CONCLUSION</h3><p>The compatibility of four effective ingredients of Danhong had protective effect toward hippocampi hypoxia. The mechanism might be related to inhibit oxidative stress damage and cell apoptosis, resist thrombosis, and reduce the intracellular calcium ion of overload.</p></div>","PeriodicalId":17513,"journal":{"name":"Journal of Traditional Chinese Medicine","volume":"38 5","pages":"Pages 685-697"},"PeriodicalIF":2.6,"publicationDate":"2018-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0254-6272(18)30907-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91448411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mixture prepared with Chinese medicines in terms of releasing exterior cold and clearing interior heat: its efficacy and safety in patients with seasonal influenza-a randomized controlled trial","authors":"Wu Zhisong ,&nbsp;Li Guodong ,&nbsp;Wei Wan ,&nbsp;Liu Zhaoheng ,&nbsp;Wang Wei ,&nbsp;Ma Ruihong ,&nbsp;Liu Wenhong ,&nbsp;Ma Jiaju ,&nbsp;Wang Yuguang ,&nbsp;Jiao Yang","doi":"10.1016/S0254-6272(18)30914-2","DOIUrl":"10.1016/S0254-6272(18)30914-2","url":null,"abstract":"<div><h3>OBJECTIVE</h3><p>To assess the efficacy and safety in 105 patients with seasonal Influenza in Beijing, the mixture prepared with Chinese medicines follows the treatment regimen of releasing exterior cold and clearing interior heat.</p></div><div><h3>METHODS</h3><p>Total 330 patients with seasonal influenza were enrolled and randomly and averagely divided into the Chinese herbal medicine, the western medicine and the Chinese patent medicine group. They were treated with Chinese medicine Oseltamivir Phosphate Capsules and the Scattering Wind and Resolving Toxins Capsules. The main efficacy indicators were the antifebrile onset time and recovery time of body temperature. The efficacy and safety of the mixture was scientifically evaluated. Comparisons of several variables were analyzed.</p></div><div><h3>RESULTS</h3><p>Median antifebrile onset time of the Chinese herbal medicine group was significantly shorter than the western medicine group (<em>P</em> &lt; 0.05) and the Chinese patent medicine group (<em>P</em> &lt; 0.05). The median antifebrile recovery time of the Chinese herbal medicine group was significantly shorter than the Chinese patent medicine group (<em>P</em> &lt; 0.05). The groups evaluated by TCM symptom pattern effect, both the Chinese herbal medicine group and Western Medicine group were better than the Chinese patent medicine group (<em>P</em> &lt; 0.05). The disappearance rate of main symptoms and some minor symptom patterns of the Chinese herbal medicine group were higher than the other 2 groups.</p></div><div><h3>CONCLUSION</h3><p>The mixture of releasing exterior cold and clearing interior heat could significantly shorten the fever time with safety.</p></div>","PeriodicalId":17513,"journal":{"name":"Journal of Traditional Chinese Medicine","volume":"38 5","pages":"Pages 746-753"},"PeriodicalIF":2.6,"publicationDate":"2018-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0254-6272(18)30914-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85192569","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effectiveness of ZiYin Xiehuo granules and Zishen Qinggan granules on partial precocious puberty in girls: a multicenter, randomized, single-blind, controlled trial","authors":"Sun Wen ,&nbsp;Han Xinghui ,&nbsp;Wang Yonghong ,&nbsp;Yu Jian ,&nbsp;Yan Weili ,&nbsp;Zhao Jun ,&nbsp;Chen Weibin ,&nbsp;Xue Zheng","doi":"10.1016/S0254-6272(18)30913-0","DOIUrl":"10.1016/S0254-6272(18)30913-0","url":null,"abstract":"<div><h3>OBJECTIVE</h3><p>To evaluate the effect of ZiYin Xiehuo granules (ZYXH) and Zishen Qinggan granules (ZSQG) on partial precocious puberty (PPP).</p></div><div><h3>METHODS</h3><p>This was a multicenter, randomized, single-blind, positive-controlled trial. A total of 143 patients were assigned to either the ZYXH group or the ZSQG group using a random number table. The ZYXH group received ZYXH three times daily for 6 months and the ZSQG group received ZSQG three times daily for 6 months. Mammary nucleus diameter; the results of uterus, ovarian, and maximum follicle measures; and Chinese medicine symptom pattern scores were compared at baseline and after 3 months and 6 months of treatment.</p></div><div><h3>RESULTS</h3><p>After 3 months’ treatment, there were no significant differences between the two groups in mammary nucleus index changes (left 3.4 ± 3.1 <em>vs</em> 3.5 ± 3.1, <em>P</em> = 0.790; right 3.0 ± 2.9 <em>vs</em> 3.6 ± 3.0, <em>P</em> = 0.719). The uterine volume in the ZYXH group was smaller than that in the ZSQG group (2.1 ± 1.6 <em>vs</em> 2.6 ± 2.2, <em>P</em> = 0.006). There were no significant between-group differences in ovarian volume and maximum follicular diameter on either side (ovarian volume: left 1.2 ± 0.7 <em>vs</em> 1.3 ± 0.6, <em>P</em> = 0.8; right 1.2 ± 0.7 <em>vs</em> 1.4 ± 1.1, <em>P</em> = 0.984; maximum follicular diameter: left 3.9 ± 1.7 <em>vs</em> 3.5 ± 2.2, <em>P</em> = 0.158; right 3.5 ± 1.7 <em>vs</em> 3.9 ± 2.1, <em>P</em> = 0.314).</p></div><div><h3>CONCLUSION</h3><p>ZYXH granules and ZSQG granules both affected the size of the mammary nucleus in girls with PPP, and improved Chinese medicine symptom patterns. ZYXH granules showed slight advantages over ZSQG granules in terms of the decrease in the size of the uterus, ovaries, and ovarian follicles.</p></div>","PeriodicalId":17513,"journal":{"name":"Journal of Traditional Chinese Medicine","volume":"38 5","pages":"Pages 740-745"},"PeriodicalIF":2.6,"publicationDate":"2018-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0254-6272(18)30913-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49601723","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pricking and penetrating moxibustion therapy in patients with refractory insomnia: a randomized and controlled clinical trial","authors":"Chen Yan ,&nbsp;Gao Xiyan ,&nbsp;Sun Cuiying","doi":"10.1016/S0254-6272(18)30915-4","DOIUrl":"10.1016/S0254-6272(18)30915-4","url":null,"abstract":"<div><h3>OBJECTIVE</h3><p>To observe the clinical effect of pricking and penetrating moxibustion therapy on refractory insomnia.</p></div><div><h3>METHODS</h3><p>Totally 60 subjects were randomly divided into treatment group and control group, 30 in each group. The treatment group was treated with pricking and penetrating moxibustion therapy, the control group with penetrating moxibustion therapy, the treatment was given once every day, two groups of patients were treated 20 times, compared the efficacy, pinsburgh sleep quality index (PSQI) and Traditional Chinese Medicine symptom, safety evaluation between the two groups.</p></div><div><h3>RESULTS</h3><p>Compared with 10, 20 times after the treatment, the effective rate of the treatment group (93.3%) higher than that of the control group (80.0%) (<em>P</em> &lt; 0.05); Compared with before treatment, 20 times after treatment, the PSQI and TCM symptom pattern scores of the two groups were significantly decreased (<em>P</em> &lt; 0.01); Compared with 10, 20 times after the treatment, the treatment group are better than the control group in improving the total score and the integral of PSQI (<em>P</em> &lt; 0.05 or <em>P</em> &lt; 0.01); The treatment group are better than the control group in improving the TCM symptom pattern score, difficulty in going to sleep, palpitation, amnesia, fidget, sweating, impaired concentration (<em>P</em> &lt; 0.01 or <em>P</em> &lt; 0.05).</p></div><div><h3>CONCLUSION</h3><p>The pricking and penetrating moxibustion therapy is safe and effective in the treatment of refractory insomnia and the treatment can improve the curative effect of the therapy that uses penetrating moxibustion only.</p></div>","PeriodicalId":17513,"journal":{"name":"Journal of Traditional Chinese Medicine","volume":"38 5","pages":"Pages 754-762"},"PeriodicalIF":2.6,"publicationDate":"2018-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0254-6272(18)30915-4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45732269","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ethyl acetate fraction in ethanol extract from root of “Dai-Bai-Jie” (Marsdenia tenacissima): anti-tumor activity in A549 cancer cells","authors":"Li Xiaohua ,&nbsp;Li Haitao ,&nbsp;Jin Lingyu ,&nbsp;Chen Leixi ,&nbsp;Niu Yingfeng ,&nbsp;Guan Yanhong ,&nbsp;Ma Xiaojun ,&nbsp;Zhang Lixia","doi":"10.1016/S0254-6272(18)30905-1","DOIUrl":"10.1016/S0254-6272(18)30905-1","url":null,"abstract":"<div><h3>OBJECTIVE</h3><p>To evaluate the anti-tumor activity of ethyl acetate fraction (EFA), extracted with ethanol from the root of “Dai-Bai-Jie” in A549 cancer cells and its underlying mechanism.</p></div><div><h3>METHODS</h3><p>“Dai-Bai-Jie” was extracted with 95% ethanol-aqueous (DBJ-1), 50% ethanol-aqueous (DBJ-2), and water (DBJ-3) by reflux method. 95% ethanol-aqueous extract was separated byethyl acetate (EFA) and n-butyl alcohol (DBJ-5), consecutively. The SRB method was used to evaluate the cytotoxic activity. Annexin V-FITC staining was applied to observe the apoptosis and analyze the cell cycle activated by EFA in A549 tumor cell. Western blot was used to detect the apoptosis/related proteins expressions. A549 tumor cellsbearing nude mice model was employed to measure the tumor volume, mice weight, and tumor inhibition ratio in order to verify the antitumor activity <em>in vivo.</em></p></div><div><h3>RESULTS</h3><p>DBJ-1 and EFA showed better cytotoxic activity on A549 tumor cells with IC₅₀ 25 and 3.5 µg/mL, respectively. EFA can exhibit the proliferation, arrest cell cycle at G0/G1 phase, and induce apoptosis in A549 tumor cells <em>in vitro.</em> The mechanisms of apoptosis induced by EFA may be associated with decreasing Bcl-2 protein expression and increasing p53, Bax, Caspase-3, and Caspase-8 proteins expression. EFA also possessed significant anti-tumor efficacy in nude mice, and little toxicity was observed in the host.</p></div><div><h3>CONCLUSION</h3><p>EAF could induce A549 tumor cells apoptosis and G0/G1 cell cycle arrest. A549 tumor cells apoptosis induced by EAF may be associated with the decrease in the ratio of Bcl-2 and Bax mRNA levels, and increase in the expression of p53, Caspase-3, and Caspase-8 proteins.</p></div>","PeriodicalId":17513,"journal":{"name":"Journal of Traditional Chinese Medicine","volume":"38 5","pages":"Pages 668-675"},"PeriodicalIF":2.6,"publicationDate":"2018-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0254-6272(18)30905-1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44573953","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}