Journal of Traditional Chinese Medicine最新文献

{"title":"Possible mechanism underlying analgesic effect of Tuina in rats may involve piezo mechanosensitive channels within dorsal root ganglia axon","authors":"Song Pengfei ,&nbsp;Lin Zhigang ,&nbsp;Zhu Qingguang ,&nbsp;Jiang Shichao ,&nbsp;Zhang Hao ,&nbsp;Cheng Yanbin ,&nbsp;Sun Wuquan ,&nbsp;Wu Zhiwei ,&nbsp;Zhou Xin ,&nbsp;Lü Zhizhen ,&nbsp;Fang Min","doi":"10.1016/S0254-6272(18)30982-8","DOIUrl":"10.1016/S0254-6272(18)30982-8","url":null,"abstract":"<div><h3>OBJECTIVE</h3><p>To demonstrate the analgesic effect of Tuina mainly from mechanically sensitive ion channels in peripheral myelinated nerve fibers.</p></div><div><h3>METHODS</h3><p>A total of 40 healthy and pathogen-free adult male Sprague-Dawley rats were used in the study [weight: (220.0 ± 1.4) g, Shanghai Slac Laboratory Animal Co., Ltd., Shanghai, China; license No. Shanghai ICP 05033115]. The rats were housed in cages with free access to water and food in a temperature-controlled room [(22 ± 1) °C] and 12-h/12-h light-dark cycle. Thirty-two rats were randomly divided into five groups: naive, sham, chronic compression of dorsal root ganglion (CCD), Tuina (7 d) and Tuina (21 d). CCD rat model was established <em>via</em> unilateral DRG compression by “L” liked steel bar. Chinese Tuina treatment was accepted once per day. Behavior monitoring of paw withdrawal threshold (PWT) and paw withdrawal latency (PWL) were tested. The expression of Piezo1 and Piezo2 in myelinated nerve fiber were analyzed by immunohistochemistry and Western-blotting.</p></div><div><h3>RESULTS</h3><p>There was a high expression of Piezo2 and a low expression of Piezo1 in the naive and CCD groups. In contrast, the expression of Piezo2 was down regulated and Piezo1 was increased after a period of Tuina. There was significant difference (<em>P</em> ≤ 0.05) between the groups.</p></div><div><h3>CONCLUSION</h3><p>Our findings suggest that Tuina therapy can increase the expression of Piezo2 and decrease the expression of Piezo1 in the test rats. The different changes in the expressions of Piezo1 and Piezo2 may play an important role in alleviating CCD-induced allodynia and hyperalgesia.</p></div>","PeriodicalId":17513,"journal":{"name":"Journal of Traditional Chinese Medicine","volume":"38 6","pages":"Pages 834-841"},"PeriodicalIF":2.6,"publicationDate":"2018-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0254-6272(18)30982-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43339975","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Involvement of heme oxygenase-1 induction in anti-vascular inflammation effects of Xanthoceras sorbifolia in human umbilical vein endothelial cells","authors":"Jung Joo Yoon ,&nbsp;Byung Hyuk Han ,&nbsp;Eun Sik Choi ,&nbsp;Seung Namgung ,&nbsp;Da Hye Jeong ,&nbsp;Song Nan Jin ,&nbsp;Yun Jung Lee ,&nbsp;Dae Gill Kang ,&nbsp;Ho Sub Lee","doi":"10.1016/S0254-6272(18)30979-8","DOIUrl":"10.1016/S0254-6272(18)30979-8","url":null,"abstract":"<div><h3>OBJECTIVE</h3><p>To define the effects of Xanthoceras sorbifolia (EXS) on vascular inflammation and the mechanisms in endothelial cells.</p></div><div><h3>METHODS</h3><p>Vascular protective effects of an ethanol extract of seeds from EXS (1-50 μg/mL) against tumor necrosis factor-α (TNF-α)-induced vascular inflammation were examined in human umbilical vein endothelial cells (HUVECs).</p></div><div><h3>RESULTS</h3><p>EXS significantly decreased TNF-α-induced expression of cell adhesion molecules, such as intracellular adhesion molecule-1, vascular cell adhesion molecule-1, and endothelial cell selectin, in a dose-dependent manner. Pre-treatment with EXS significantly inhibited translocation and transcriptional activity of nuclear factor-κB (NF-κB) increased by TNF-α. EXS also significantly inhibited formation of intracellular reactive oxygen species (ROS). Moreover, the vascular protective effects of EXS were linked to up-regulation of heme oxygenase-1 (HO-1) and nuclear factor E2-related factor-2 (Nrf-2) expression. EXS-induced HO-1 expression was significantly decreased in SnPP (HO-1 inhibitor)- and HO-1 siRNA-treated cells, whereas an increase was found in cobalt protoporphyrin IX (CoPP) (HO-1 inducer)-treated cells. In addition, pretreatment with EXS increased HO-1 and Nrf-2 expression under TNF-α stimulation with or without N-acetyl-L-cysteine. Furthermore, the inhibitory effects of EXS on TNF-α-induced vascular inflammation were partially reversed in SnPP- and of HO-1 siRNA-treated cells but increased by CoPP.</p></div><div><h3>CONCLUSION</h3><p>These results suggest that EXS may have important implications for prevention of vascular complications associated with vascular inflammation by inhibition of the NF-κB/ROS pathway and activation of the Nrf-2/HO-1 pathway.</p></div>","PeriodicalId":17513,"journal":{"name":"Journal of Traditional Chinese Medicine","volume":"38 6","pages":"Pages 803-814"},"PeriodicalIF":2.6,"publicationDate":"2018-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0254-6272(18)30979-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42460618","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Inhibitive effect of loureirin B plus capsaicin on tetrodotoxin-resistant sodium channel","authors":"Chen Su ,&nbsp;Wan Ying ,&nbsp;Liu Xiangming ,&nbsp;Pan Xinxin","doi":"10.1016/S0254-6272(18)30983-X","DOIUrl":"10.1016/S0254-6272(18)30983-X","url":null,"abstract":"<div><h3>OBJECTIVE</h3><p>To investigate whether the effect of loureirin B plus capsaicin on tetrodotoxin-resistant (TTX-R) sodium channel.</p></div><div><h3>METHODS</h3><p>By using whole-cell patch-clamp recordings, in acutely isolated dorsal root ganglion (DRG) neurons, the combined effects of loureirin B and capsaicin on TTX-R sodium channel were observed. Based on the data, the interaction between loureirin B and capsaicin in their modulation on TTX-R sodium channel was assessed.</p></div><div><h3>RESULTS</h3><p>Loureirin B could not induce transient inward TRPV1 current. Capsazepine, a transient receptor potential vanilloid l (TRPV1) antagonist, could not attenuate the block of 0.64 mmol/L loureirin B on TTX-R sodium channel. There was no significant difference (<em>P</em> &gt; 0.05) between IC₅₀ of loureirin B (0.37 mmol/L) on TTX-R sodium channel in capsaicin-sensitive DRG neurons and that (0.38 mmol/L) in capsaicin-insensitive DRG neurons. However, there was a significant difference (<em>P</em> &lt; 0.05) between the IC₅₀ of capsaicin (0.28 μmol/L) on TTX-R sodium channel in capsaicin-sensitive DRG neurons and that (52.24 μmol/L) in capsaicin-insensitive DRG neurons. Four combinations composed of various concentrations of loureirin B and capsaicin could all inhibit TTX-R sodium currents but have different interactions between loureirin B and capsaicin.</p></div><div><h3>CONCLUSION</h3><p>Loureirin B plus capsaicin could produce double blockage on TRPV1 and modulation on TTX-R sodium channel. The action of loureirin B on TTX-R sodium channel was independent of TRPV1 but similar with that of capsaicin on TTX-R sodium channel in capsaicin-insensitive DRG neurons.</p></div>","PeriodicalId":17513,"journal":{"name":"Journal of Traditional Chinese Medicine","volume":"38 6","pages":"Pages 842-852"},"PeriodicalIF":2.6,"publicationDate":"2018-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0254-6272(18)30983-X","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47259914","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protein losing enteropathy caused by eosinophilic gastroenteritis: A case report","authors":"Zhu Jiajie ,&nbsp;Liu Shan ,&nbsp;Su Xiaolan ,&nbsp;Guo Yu ,&nbsp;Li Yijie ,&nbsp;Chen Jiande ,&nbsp;Wang Qingguo ,&nbsp;Wei Wei","doi":"10.1016/S0254-6272(18)30996-8","DOIUrl":"10.1016/S0254-6272(18)30996-8","url":null,"abstract":"<div><p>Protein losing enteropathy (PLE), a very rare disease with hypoproteinemia and edema as its characteristics, is caused by various diseases resulting in protein depletion from the gut. The diagnosis is relatively difficult due to its complex pathogeneses. The present paper reported a case whose symptom started with acute diarrhea and hypoproteinemia. Gastrointestinal endoscopies showed digestive ulcers and colon polyp. The treatments contained albumin infusion, Chinese herbal decoction and other symptomatic therapies. The hypoproteinemia become even worse and edema occurred after 4 days' treatment. A larger dose of albumin infusion (40-60 g/d) and modified herbal decoctions were prescribed. A final diagnosis of eosinophilic gastroenteritis (EG) complicated with PLE was confirmed by histopathological examination of a repeated gastroscopy. After three weeks' treatment, the serum albumin level was raised and the edema subsided gradually. In conclusion, herbs may have an effect on PLE patients, but PLE resulting from EG is very complex and easy to misdiagnose, especially in atypical conditions. Further studies are required to find the exact mechanisms.</p></div>","PeriodicalId":17513,"journal":{"name":"Journal of Traditional Chinese Medicine","volume":"38 6","pages":"Pages 953-957"},"PeriodicalIF":2.6,"publicationDate":"2018-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0254-6272(18)30996-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87090064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of esculetin on bone metabolism in ovariectomized rats","authors":"Liu Meijie ,&nbsp;Wang Ruihai ,&nbsp;Li Yan ,&nbsp;Bai Dong ,&nbsp;Pan Jinghua ,&nbsp;Liu Hong ,&nbsp;Wang Shaojun ,&nbsp;Wu Jiaying ,&nbsp;Sun Gang ,&nbsp;Miao Qing ,&nbsp;Ju Dahong ,&nbsp;Liu Limei","doi":"10.1016/S0254-6272(18)30989-0","DOIUrl":"10.1016/S0254-6272(18)30989-0","url":null,"abstract":"<div><h3>OBJECTIVE</h3><p>To determine the effect of an esculetin formulation (at 97.4% purity) on osteoporosis, and to investigate the potential underlying molecular mechanism(s).</p></div><div><h3>METHODS</h3><p>Sixty specific pathogen free-grade female Wistar rats were randomly assigned to three groups: blank control (<em>n =</em> 12), sham (<em>n =</em> 12), and model (<em>n =</em> 36). The model group were bilaterally ovariectomized. The sham group had the tissue surrounding the ovaries removed, while the ovaries were retained. After 3 months, the model group was randomly divided into three subgroups: OVX (<em>n =</em> 12), positive control (<em>n =</em> 12), and esculetin (<em>n =</em> 12). The positive control group and the esculetin group were intragastrically administered diethylstilbestrol (0.046 mg • kg⁻¹ • d⁻¹) or esculetin (384 mg • kg⁻¹ • d⁻¹), respectively, once per day for 6 consecutive days; medication administration was then stopped for 1 d, before being administered for another 6 consecutive days. All rats were treated for 3 months. Samples were collected at the end of the treatment period. An Osteocore3 Digital 2D bone densitometer was used to test the bone mineral density, and histomorphometric analysis was performed to measure bone mass, bone formation, and bone resorption. Enzyme-linked immunosorbent assay analysis was used to measure the serum concentrations of interleukin-6 (IL-6), osteoprotegerin (OPG), and receptor activator of nuclear factor-kappa B ligand (RANKL). Immunohistochemistry and in situ hybridization were performed to detect the protein and mRNA expressions of OPG and RANKL in osteoblasts and bone marrow stromal cells.</p></div><div><h3>RESULTS</h3><p>Compared with the OVX group, the esculetin group had significantly greater femoral bone mineral density and tibial trabecular bone volume, and significantly smaller trabecular resorption surface. The percentage of trabecular formation surface, average osteoid width, trabecular bone mineralization rate, and cortical bone mineralization rate did not significantly differ between groups. Compared with the sham group, the esculetin group had significantly decreased serum levels of IL-6 and RANKL, and significant downregulation of RANKL protein and mRNA expression levels in osteoblasts and bone marrow stromal cells; however, there was no significant difference between groups in OPG.</p></div><div><h3>CONCLUSION</h3><p>Esculetin can increase bone mass by upregulating RANKL expression in osteoblasts and bone marrow stromal cells, and decreasing serum IL-6 concentration. This indicates that the therapeutic effect of esculetin on osteoporosis occurs <em>via</em> decreased bone resorption.</p></div>","PeriodicalId":17513,"journal":{"name":"Journal of Traditional Chinese Medicine","volume":"38 6","pages":"Pages 896-903"},"PeriodicalIF":2.6,"publicationDate":"2018-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0254-6272(18)30989-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47969891","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Yupingfeng granules on the skin barrier in atopic dermatitis mice models","authors":"Sun Zhanxue ,&nbsp;Wang Jingjun ,&nbsp;Kong Yuhong ,&nbsp;Li Nan ,&nbsp;Jiang Xiaoyuan ,&nbsp;Cao Ting ,&nbsp;Jia Yuanning ,&nbsp;Zhang Yueyue ,&nbsp;Zhang Yisheng ,&nbsp;Cheng Jing","doi":"10.1016/S0254-6272(18)30986-5","DOIUrl":"10.1016/S0254-6272(18)30986-5","url":null,"abstract":"<div><h3>OBJECTIVE</h3><p>To investigate effect of Yupingfeng granules, prepared with Chinese Medicines, on the wound healing and on the expression of aquaporin 3 (AQP3) and the skin barrier in the animal models of atopic dermatitis (AD).</p></div><div><h3>METHODS</h3><p>Acute skin lesions of AD models were prepared using 2,4-dinitrochlorobenzo (DNCB) in mice and animals were treated with either Yupingfeng granules or placebo for two weeks. Skin wound healing outcome was assessed by measuring skin thickness, weight (quality) of the skin, and trans-epidermal water loss (TEWL). Expression of AQP3 mRNA and protein was assessed by reverse transcriotion polymerase chain reaction (RT-PCR) and immunoblotting, respectively.</p></div><div><h3>RESULTS</h3><p>Yupingfeng granule treatment resulted in significant acceleration of wound healing with 63.64% efficiency, which was significantly higher than that of placebo granule treatment (31.82%, <em>P</em> &lt; 0.01 by Wilcoxon Rank-sum test). Skin thickness, weight of the wounded skin, and TEWL were significantly higher in the AD models compared to that of normal animals. Treatment with Yupingfeng granules resulted in significant decrease in skin thickness [(937 ±31) <em>vs</em> (360 ±21) urn, <em>P</em> &lt; 0.01], weight of the wounded skin [(42 ± 4) <em>vs</em> (24 ± 5) mg, <em>P</em> &lt; 0.01], and TEWL [(30 ±4) <em>vs</em> (13 ± 4) g • h⁻¹ • m⁻², <em>P</em> &lt; 0.01]. Yupingfeng granules also significantly down-regulated mRNA and protein expression of AQP3 in the animal models.</p></div><div><h3>CONCLUSION</h3><p>Our findings suggested that Yupingfeng granules could be used in AD treatment.</p></div>","PeriodicalId":17513,"journal":{"name":"Journal of Traditional Chinese Medicine","volume":"38 6","pages":"Pages 872-878"},"PeriodicalIF":2.6,"publicationDate":"2018-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0254-6272(18)30986-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46735362","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Shenqi Yangxin decoction on high mobility group box 1 and inflammatory signal pathway in a rat model of dilated cardiomyopathy","authors":"Shen Lijuan ,&nbsp;Lu Shu ,&nbsp;Zhou Yonghua ,&nbsp;Xing Qingmin ,&nbsp;Zhou Chungang ,&nbsp;Li Lan","doi":"10.1016/S0254-6272(18)30985-3","DOIUrl":"10.1016/S0254-6272(18)30985-3","url":null,"abstract":"<div><h3>OBJECTIVE</h3><p>To investigate the effects of Shenqi Yangxin decoction (SQYXD) on heart function in a rat model of dilated cardiomyopathy (DCM) and its potential mechanisms.</p></div><div><h3>METHODS</h3><p>Sprague-Dawley rats were randomly divided into normal (10 rats) and DCM (150 rats) groups. DCM was induced by an intraperitoneal injection of adriamycin. Then, DCM baseline group was randomly selected sixteen DCM rats. The remaining DCM rats were randomly divided into DCM control, perindopril, metoprolol, and SQYXD groups. Cardiac function and histological analysis plus biochemical measurement of serum levels of brain natriuretic peptide (BNP), and inflammatory factors were measured. The mRNA and protein expression levels of high-mobility group box 1 (HMGB1), Toll-like receptor 4 (TLR-4), receptor for advanced glycation end products (RAGE), and nuclear factor-κB (NF-κB) were determined. Myocardial metabolism imaging was performed on the normal, SQYXD and DCM control groups to evaluate the effectiveness of treatments.</p></div><div><h3>RESULTS</h3><p>Rats in the DCM control group exhibited dilated left ventricular diameter, impaired cardiac function, disorganized sarcomere, impaired glucose metabolism, increased heart weight index, and increased levels of BNP, which were improved by treatment with SQYXD. In addition, hearts from rats in the DCM baseline group exhibited significantly higher levels of HMGB1, TLR-4, RAGE, NF-κB, tumor necrosis factor-α, interleukin-1, interleukin-6, interleukin-10, compared with the normal group. Interestingly, the mRNA level of HMGB1 in the DCM baseline group was positively correlated with that of TLR-4, RAGE, NF-κB, BNP, and LVEDD, but negatively correlated with LVEF. SQYXD inhibited the upregulation of HMGB1 expression and its downstream inflammatory factors.</p></div><div><h3>CONCLUSION</h3><p>Shenqi Yangxin decoction effectively reduced the dilated left ventricular diameter and improved heart function in dilated cardiomyopathy. The mechanisms underlying the action on DCM involve regulating the gene and protein expression of HMGB1 and its inflammatory signal pathways in the DCM rat model.</p></div>","PeriodicalId":17513,"journal":{"name":"Journal of Traditional Chinese Medicine","volume":"38 6","pages":"Pages 862-871"},"PeriodicalIF":2.6,"publicationDate":"2018-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0254-6272(18)30985-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41434540","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A model for anticancer surveillance was pharmacologically developed to evaluate vitality principle in breast cancer rats","authors":"Liu Jinyu ,&nbsp;Zhang Mengyang ,&nbsp;Zhao Xin ,&nbsp;Ge Shasha ,&nbsp;Li Shuang ,&nbsp;Peng Lin ,&nbsp;Mu Yuxue ,&nbsp;Chen Chen ,&nbsp;Li Xiaoya ,&nbsp;Zhang Rui ,&nbsp;Feng Xuanye ,&nbsp;Deng Bo ,&nbsp;Jia Liqun ,&nbsp;Lin Yulin ,&nbsp;Wang Yueqi ,&nbsp;Cheng Zhiqiang ,&nbsp;TaeHoo Yi ,&nbsp;Cai Dayong","doi":"10.1016/S0254-6272(18)30981-6","DOIUrl":"https://doi.org/10.1016/S0254-6272(18)30981-6","url":null,"abstract":"<div><h3>OBJECTIVE</h3><p>To evaluate vitality principle in breast cancer rats by pharmacologically developing a model for anticancer surveillance.</p></div><div><h3>METHODS</h3><p>The breast cancer in rats was replicated with 7,12-Dimethylbenz[a]anthracene (DMBA, i.g., 100 mg/kg) at d₀₀₁. The anticancer surveillance was defined as the intervals between the primary sensitization and the first challenge stirred with complete Freund's adjuvant (CFA), the various intervals (k = 0.80) were dominated from d₀₂₅ (600.00 h) to d₀₉₅ (2288.82 h). The optimal surveillant status was confirmed with the median effective interval (EI₅₀) from tumor volume regressive curve, for developing the pharmacodynamic model. The tumor and tumor infiltrating lymphocyte histopathology was used to confirm the immune surveillance being affected with CFA in breast cancer tumorigenesis. The availability of this model was confirmed with Shugan Liangxue prescription (SLP), from the vitality principle, and assured further from interleukin-12 levels.</p></div><div><h3>RESULTS</h3><p>The regressive curve was set up between the intervals and tumor volumes, the EI₅₀ in SLP-treated rats (1475.00 h, Y_SLP = 0.1026 + 0.8780/[1 + 10^{(27.1425-8.565×)}]) was postponed, which was 1.87 multiple of the EI₅₀ in CFA rats (791.40 h, y = −0.0525 + 0.9452/[1 + 10^{(30.4870-10.52×)}], so did prepone the curve between the intervals and the immunological biomarker, serum inter-leukin-12 levels, the EI₅₀ in SLP-treated rats (744.90 h, Y_SLP = −0.0145 + 0.7455/[1 + 10^{(52.09636-18.13×)}]) be 0.78 multiple of the EI₅₀ in CFA rats (960.10 h, Y_CFA = 0.2460 + 0.7270/[1 + 10^{(− 67.1546} ⁺ ^22.52×)]), this immunological action being mediated the anticancer prognosis. Tumor histology was confirmed the more tumor infiltrating lymphocytes activated in SLP rats with CFA stirred immunity than rats only received CFA.</p></div><div><h3>CONCLUSION</h3><p>The model for anticancer surveillance was pharmacologically established as the optimal interval (791.40 h) between the primary sensitization and the first challenge stirred with complete Freund's adjuvant. This available model was confirmed with SLP, from the vitality principle, for evaluating immunological effects against breast cancer.</p></div>","PeriodicalId":17513,"journal":{"name":"Journal of Traditional Chinese Medicine","volume":"38 6","pages":"Pages 823-833"},"PeriodicalIF":2.6,"publicationDate":"2018-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0254-6272(18)30981-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72261251","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Risk assessment model for endpoints of ischemic stroke: A study protocol for a registry study","authors":"Zhang Yin ,&nbsp;Liu Yue ,&nbsp;Wang Guiqian ,&nbsp;Sun Shuailing ,&nbsp;Gao Yang ,&nbsp;Xie Yanming","doi":"10.1016/S0254-6272(18)30994-4","DOIUrl":"10.1016/S0254-6272(18)30994-4","url":null,"abstract":"<div><h3>OBJECTIVE</h3><p>To evaluate the risk of ischemic stroke endpoints by establishing risk assessment models that combine Traditional Chinese Medicine (TCM) and modern medicine indicators.</p></div><div><h3>METHODS/DESIGN</h3><p>The proposed study is a registry-based participant survey conducted in seven hospitals nationwide in China. After obtaining informed consent, 3000 patients diagnosed with ischemic stroke will be recruited. One-year follow-ups will be performed on-site in hospitals and by telephone to track endpoint events. Comparative analysis of the prevalence of endpoint events and other TCM or modern medicine features in different groups will be conducted using frequency analysis and χ<span>²</span> tests, and the results will be expressed as composition ratios. Comparative analysis of quantitative scores and related patterns or symptoms will be conducted using a rank-sum test. Correlation analysis of endpoint events and TCM or modern medicine factors will be performed using a multivariate Cox proportional hazard model.</p></div><div><h3>DISCUSSION</h3><p>Previous reports have described modern medicine indicator-based risk assessment models for ischemic stroke endpoint events, but no such studies have included TCM features. Our new risk assessment model combines TCM and modern medicine indicators and thus has the potential to facilitate early warning, early intervention, and early control of ischemic stroke endpoint events.</p></div>","PeriodicalId":17513,"journal":{"name":"Journal of Traditional Chinese Medicine","volume":"38 6","pages":"Pages 936-942"},"PeriodicalIF":2.6,"publicationDate":"2018-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0254-6272(18)30994-4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48554689","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Jianpi Bushen formula on aromatase-inhibitor-associated bone loss after menopause","authors":"Dai Yan ,&nbsp;Chen Qingqing ,&nbsp;Guan Ruodan ,&nbsp;Xu Rui ,&nbsp;Qiu Chang ,&nbsp;Song Xue ,&nbsp;Guo Qianqian ,&nbsp;Wang Zhiyu ,&nbsp;Chen Qianjun","doi":"10.1016/S0254-6272(18)30987-7","DOIUrl":"10.1016/S0254-6272(18)30987-7","url":null,"abstract":"<div><h3>OBJECTIVE</h3><p>To investigate the effect of Jianpi Bushen (JPBS) formula on aromatase inhibitor (AI)-associated bone loss after menopause.</p></div><div><h3>METHODS</h3><p>Six-month-old female rats were randomly divided into 6 groups: a sham group, an ovariectomized (OVX) group, an OVX treated with exemestane and 3 OVX groups each treated with a different dose of JPBS formula. Bone mineral density (BMD) at the lumbar vertebrae, histology, bone markers and serum levels of estrogen were assessed. Furthermore, a cohort study was conducted in 130 postmenopausal women with breast cancer that had undergone treatment with Als. The subjects were given JPBS + caltrate D or caltrate D only, administered orally. BMD at the lumbar vertebrae and femoral neck and bone markers were evaluated in both control and herbal treatment groups at baseline and 12 months.</p></div><div><h3>RESULTS</h3><p>Experimental results indicated that a high dose of JPBS significantly increased the trabecular bone area percentage (Tb.Ar %) and broadened the trabecular thickness (Tb.Th). The JPBS formula enriched the carboxyterrninal propeptide of type ipmcollagen and increased serum estrogen level significantly. The clinical investigation revealed that bone loss was decreased in the group treated with JPBS <em>vs</em> control (BMD T score at lumbar vertebrae, 3.9% increased <em>vs</em> 14.58% decreased, respectively, <em>P</em> = 0.004 and BMD T score on femoral neck, 1.8% decreased <em>vs</em> 22.45% decreased, respectively, <em>P</em> = 0.008). Besides, JPBS formula elevated N-middle osteocalcin and decreased type I collagen cross-linked C-terminal telopeptide.</p></div><div><h3>CONCLUSION</h3><p>JPBS formula prevented aromatase-inhibitor-associated bone loss after menopause by inhibiting bone resorption and promoting bone formation.</p></div>","PeriodicalId":17513,"journal":{"name":"Journal of Traditional Chinese Medicine","volume":"38 6","pages":"Pages 879-889"},"PeriodicalIF":2.6,"publicationDate":"2018-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0254-6272(18)30987-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45725411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}