Journal of the Reticuloendothelial Society最新文献_第7页

The activation of tumoricidal properties in macrophages of endotoxin responder and nonresponder mice by liposome-encapsulated immunomodulators. 脂质体包封免疫调节剂对内毒素应答和无应答小鼠巨噬细胞杀瘤特性的激活作用。

Journal of the Reticuloendothelial Society Pub Date : 1983-03-01

W E Fogler, J E Talmadge, I J Fidler

{"title":"The activation of tumoricidal properties in macrophages of endotoxin responder and nonresponder mice by liposome-encapsulated immunomodulators.","authors":"W E Fogler, J E Talmadge, I J Fidler","doi":"","DOIUrl":"","url":null,"abstract":"The purpose of these studies was to determine whether various immunomodulators such as lipopolysaccharide (LPS), lymphokines with macrophage activation factor (MAF), or muramyl dipeptide (MDP) could activate the tumoricidal properties in LPS-responsive C3H/HeN and LPS-unresponsive C3H/HeJ mice. In all studies we examined the interaction of the different immunomodulators in a free form or encapsulated within liposomes (multilamellar vesicles) with alveolar macrophages (AM) and/or peritoneal exudate macrophages (PEM). In vivo infection with viable Mycobacterium bovis, strain BCG, induced the development of highly activated macrophages from C3H/HeN mice, yet only marginally activated macrophages from C3H/HeJ mice. In vitro incubation with MAF or LPS rendered AM and PEM from C3H/HeN, but not C3H/HeJ, mice tumoricidal. The failure of C3H/HeJ macrophages to respond to LPS stimulation was due to an intracellular defect. C3H/HeJ macrophages bound fluorescein-conjugated LPS to the same extent as that found for C3H/HeN macrophages. Furthermore, LPS encapsulated in liposomes activated C3H/HeN but not C3H/HeJ AM and/or PEM. Macrophages from both strains could be rendered highly tumoricidal following interaction with free MDP or following endocytosis of liposomes containing MDP or MAF. These results indicate that the inability of C3H/HeJ macrophages to respond to LPS stimulation is specific and that the activation of macrophages by different immunomodulators could occur by different pathways.","PeriodicalId":17481,"journal":{"name":"Journal of the Reticuloendothelial Society","volume":"33 3","pages":"165-74"},"PeriodicalIF":0.0,"publicationDate":"1983-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17886169","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of local and systemic macrophage activation in hamsters on infection with Treponema pertenue and Treponema pallidum Bosnia A. 仓鼠局部和全身巨噬细胞活化对梅毒螺旋体和梅毒螺旋体感染的影响。

Journal of the Reticuloendothelial Society Pub Date : 1983-03-01

R F Schell, A A Azadegan, S G Nitskansky, J L LeFrock

{"title":"Effect of local and systemic macrophage activation in hamsters on infection with Treponema pertenue and Treponema pallidum Bosnia A.","authors":"R F Schell, A A Azadegan, S G Nitskansky, J L LeFrock","doi":"","DOIUrl":"","url":null,"abstract":"The role of nonspecific macrophage activation in the destruction of treponemes needs to be defined. Studies have been hindered by an inability to confirm that macrophages have enhanced bactericidal activity at the site of treponemal infection. We show that subcutaneous and intravenous vaccination with BCG (Mycobacterium bovis) induces macrophage activation in hamsters, as determined by an enhanced ability to suppress the growth of Listeria monocytogenes in the livers, spleens, and inguinal lymph nodes. However, hamsters challenged in the inguinal region with Treponema pertenue during periods of enhanced microbial resistance (3 to 8 weeks after BCG vaccination) developed lesions faster and with more necrosis. Increased numbers of treponemes were recovered from the regional lymph nodes of BCG-vaccinated hamsters than from nonvaccinated controls, although the differences were not statistically significant. No pathological differences were detected in BCG-vaccinated and non-vaccinated hamsters challenged with Treponema pallidum Bosnia A. These studies demonstrate that BCG vaccination influences the pathogenesis of some treponemal diseases without inducing macrophage-mediated treponemicidal activity.","PeriodicalId":17481,"journal":{"name":"Journal of the Reticuloendothelial Society","volume":"33 3","pages":"231-8"},"PeriodicalIF":0.0,"publicationDate":"1983-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17886170","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Macrophage subpopulations and the murine F1 x parent mixed leukocyte reaction. 巨噬细胞亚群与小鼠F1 x亲本混合白细胞的反应。

Journal of the Reticuloendothelial Society Pub Date : 1983-03-01

J C Gural, W S Walker

{"title":"Macrophage subpopulations and the murine F1 x parent mixed leukocyte reaction.","authors":"J C Gural, W S Walker","doi":"","DOIUrl":"","url":null,"abstract":"The ability of subpopulations of murine spleen cells enriched for macrophages to function as stimulator cells in the F1 x parent-hybrid mixed leukocyte reaction (F1 x P MLR) was assessed. Suspensions of F1 splenic adherent cells--depleted of T cells, B cells, and dendritic cells--were separated into four density-dependent subpopulations on discontinuous gradients of Percoll, and the cells were examined for macrophage characteristics, the presence of la antigen, and their ability to stimulate in the F1 x P MLR. The least dense subpopulation was the most highly enriched with nonspecific esterase-positive (NSE+), phagocytic, and Fc and complement receptor-bearing cells, by comparison with the denser subpopulations. All subpopulations contained similar proportions of Ia+ cells. When the MLR stimulatory activity of the subpopulations was tested with suspensions of parental responder cells, the level of stimulation was directly proportional to the content of NSE+la+ cells in the subpopulations. The densest subpopulation contained no NSE+ cells but did contain la+ cells, which did not induce a MLR. Thus, although necessary for an MLR, la-bearing splenocytes were not by themselves adequate to stimulate the reaction. Rather, cells had to be both NSE+ and la+, indicating that a NSE+ cell-derived factor is involved in this in vitro correlate of cell-mediated immunity. For MLR stimulatory activity, the subpopulations of NSE+la+ cells appear to be functionally homogeneous.","PeriodicalId":17481,"journal":{"name":"Journal of the Reticuloendothelial Society","volume":"33 3","pages":"185-95"},"PeriodicalIF":0.0,"publicationDate":"1983-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17284023","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Macrophage-like cell lines: endogenous peroxidatic activity, cell surface antigens, and colony-stimulating factor production. 巨噬细胞样细胞系:内源性过氧化物活性、细胞表面抗原和集落刺激因子的产生。

Journal of the Reticuloendothelial Society Pub Date : 1983-03-01

H Van Loveren, J Hilgers, J M De Bakker, R A De Weger, P Brederoo, W Den Otter

引用次数: 0

On the origin of macrophage heterogeneity: a hypothesis. 巨噬细胞异质性的起源:一个假说。

Journal of the Reticuloendothelial Society Pub Date : 1983-03-01

I Bursuker, R Goldman

引用次数: 0

Differential effects of glutaraldehyde treatment of target cells on lectin-dependent macrophage-mediated tumor lysis. 戊二醛处理靶细胞对凝集素依赖性巨噬细胞介导的肿瘤溶解的不同影响。

Journal of the Reticuloendothelial Society Pub Date : 1983-03-01

N Iwata-Dohi, M Esumi-Kurisu, D Mizuno, M Yamazaki

引用次数: 0

The effect of macrophages on the metabolism of glomerular cells: preliminary studies. 巨噬细胞对肾小球细胞代谢的影响:初步研究。

Journal of the Reticuloendothelial Society Pub Date : 1983-02-01

C M Wagner, D O Lucas, R B Nagle

{"title":"The effect of macrophages on the metabolism of glomerular cells: preliminary studies.","authors":"C M Wagner, D O Lucas, R B Nagle","doi":"","DOIUrl":"","url":null,"abstract":"Human glomerular epithelial and mesangial cells were grown in vitro and shown to have distinctive morphologic and functional characteristics. Glomerular epithelial cells or mesangial cells cultured in wells of flat-bottom microtiter plates were treated for 4 hr with dialyzed macrophage supernatants obtained from cultures of mouse peritoneal macrophages or human peripheral monocytes. DNA, RNA, and protein synthesis were evaluated by incorporation of radioactive precursors. Macrophage supernatants stimulated RNA and protein synthesis in epithelial cells but failed to stimulate DNA synthesis. The macrophage factor(s) showed a dose-response activity, was nondialyzable, was destroyed by freezing and thawing, and did not seem to be species specific. In contrast to the results obtained with glomerular epithelial cells, mesangial cell DNA synthesis was stimulated by macrophage supernatants. The observed metabolic effects of macrophage products on glomerular cells in vitro are consistent with observations of in vivo glomerular response to injury in which epithelial cells may be activated to form new basement membrane while mesangial cells may respond by proliferating. These data further support the theory of macrophage involvement in the pathology of glomerulonephritis.","PeriodicalId":17481,"journal":{"name":"Journal of the Reticuloendothelial Society","volume":"33 2","pages":"93-107"},"PeriodicalIF":0.0,"publicationDate":"1983-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17284411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Separation of bronchoalveolar cells from the guinea pig on continuous gradients of Percoll: functional properties of fractionated lung macrophages. 支气管肺泡细胞在Percoll连续梯度下的分离:分离肺巨噬细胞的功能特性。

Journal of the Reticuloendothelial Society Pub Date : 1983-02-01

A Holian, J H Dauber, M S Diamond, R P Daniele

引用次数: 0

Mechanism of follicular trapping: similarities and differences in trapping of antibody-complexed antigens and carbon particles in the follicles of the spleen. 滤泡诱捕机制:脾脏滤泡中抗体复合抗原和碳颗粒诱捕的异同。

Journal of the Reticuloendothelial Society Pub Date : 1983-02-01

P H Groeneveld, P Eikelenboom, N van Rooijen

引用次数: 0

Separation of bronchoalveolar cells from the guinea pig on continuous density gradients of Percoll: morphology and cytochemical properties of fractionated lung macrophages. 连续Percoll密度梯度下豚鼠支气管肺泡细胞的分离:肺巨噬细胞的形态学和细胞化学性质。

Journal of the Reticuloendothelial Society Pub Date : 1983-02-01

J H Dauber, A Holian, M E Rosemiller, R P Daniele

{"title":"Separation of bronchoalveolar cells from the guinea pig on continuous density gradients of Percoll: morphology and cytochemical properties of fractionated lung macrophages.","authors":"J H Dauber, A Holian, M E Rosemiller, R P Daniele","doi":"","DOIUrl":"","url":null,"abstract":"Cells recovered by lavage from lungs of normal guinea pigs were centrifuged on continuous density gradients of colloidal silica (Percoll). The gradient was divided into six fractions based on the banding pattern of cells. This pattern was highly reproducible from animal to animal. Cell types in the fractions were identified by morphological and cytochemical criteria and the volume of the cells was determined by measuring their diameter and tritiated water space. More than 70% of the cells put on the gradient were recovered in the six fractions and there was no selective loss of cell types. Macrophages comprised more than 95% of the cells in fractions 3, 4, and 5. These fractions were of intermediate density (1.037-1.078 gm/ml) and together contained more than 85% of the recovered macrophages. Fraction 6 (density 1.078-1.130 gm/ml) was enriched for lymphocytes and granulocytes. Macrophages in fraction 5 were smaller, had more densely staining cytoplasm, and exhibited more nonspecific cytoplasmic esterase activity than macrophages in other fractions (5 greater than 4 greater than 3 greater than 2). These results indicate that density-gradient centrifugation on Percoll is an efficient method for purifying guinea pig alveolar macrophages and demonstrate that macrophages that differ in bouyant density also differ in morphologic and cytochemical properties. In a companion paper we report that macrophages in fractions 3, 4, and 5 differ functionally as well [9].","PeriodicalId":17481,"journal":{"name":"Journal of the Reticuloendothelial Society","volume":"33 2","pages":"119-26"},"PeriodicalIF":0.0,"publicationDate":"1983-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17359417","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0